84 results about "Metabolic waste" patented technology

The invention relates to an automatic peritoneal dialysis machine auxiliary system capable of preventing peritoneal protein from being lost, and belongs to the technical field of peritoneal dialysis. By arranging the auxiliary system comprising a hollow fiber membrane filter, peritoneal drainage liquid is guided into the hollow fiber membrane filter in the drainage stage; the protein is intercepted by utilizing the semi-permeable membrane characteristic, and small molecule metabolic wastes penetrate through the semi-permeable membrane characteristic to form a filtrate rich in the protein; and then the feedback pump speed is dynamically adjusted through a control system based on a signal of a flow sensor, so that the feedback process of the protein liquid is stably synchronized with drainage, liquid injection and staying stages of the automatic peritoneal dialysis machine, online recovery and safe feedback of protein are realized, and the problem that the protein liquid flows back to the treatment cycle of the automatic peritoneal dialysis machine is solved on the premise that the treatment cycle is coordinated with the treatment cycle of the automatic peritoneal dialysis machine. Protein in peritoneal drainage liquid is continuously and selectively recovered and safely returned, so that the problem of protein trophism loss of long-term peritoneal dialysis patients is directly reduced.

The invention belongs to the field of medical equipment, and provides an organ perfusion device, which comprises a liquid storage bin, a perfusion device and a control device, the organ bin is used for storing isolated organs; the pump is used for driving perfusate; the perfusion pipeline is used for perfusion of an isolated organ, is provided with two or more flow division pipelines and is used for perfusion and flow division; the temperature maintaining system is used for maintaining the perfusate and the isolated organ at different temperatures; the oxygenator is used for oxygenating the perfusate; the central control system is used for system control; the perfusion pipeline is driven by the pump to extract perfusion liquid from the liquid storage bin to perfuse the isolated organ in the organ bin. Metabolic waste can be prevented from entering circulation again through one-way perfusion, low-temperature and normal-temperature double-phase automatic switching is achieved, one-way tidal perfusion is achieved, the device structure is obviously simplified, and the overall performance such as safety, stability and portability is improved.

The invention provides a dynamic regulation and control method for optimizing CAR-T cell amplification conditions, which comprises the following steps: acquiring high-frequency cell activity monitoring data from a cell culture system, and extracting metabolic marker fluctuation and proliferation rate mutation from the high-frequency cell activity monitoring data to obtain real-time cell state parameters; comparing the real-time cell state parameters with a T cell subset proportion and a cell factor release mode in historical immune data of the patient to obtain a cell state deviation value, and determining an abnormal fluctuation condition of proliferation rate mutation according to the cell state deviation value; if the abnormal fluctuation of the proliferation rate mutation exceeds a preset fluctuation threshold value, capturing proliferation signal abnormity by analyzing metabolic marker fluctuation and metabolic waste accumulation according to the cell state deviation value; according to the targeted culture parameter set, adjusting the oxygen supply quantity and the culture solution microenvironment of an oxygen concentration control system to obtain a culture environment for inhibiting cytokine storm and proliferation signal abnormity.

This invention discloses a composition with whitening and freckle-removing effects, its preparation method, and its application, belonging to the field of composition technology. The composition provided by this invention reduces the body's oxidative stress level, providing a suitable internal microenvironment for the efficacy of various drugs and the repair of damage; it provides a sufficient, diverse, and easily absorbed material basis for skin damage repair; it significantly improves the whitening and freckle-removing effect by increasing the activity, purity, and content of the components that directly have whitening and freckle-removing effects; and it promotes the excretion of metabolic waste by improving gastrointestinal health. It is extremely safe, requires minimal equipment, and is environmentally friendly, making it suitable for daily skin repair and whitening / freckle-removing edible use.

The invention discloses a whitening composition as well as a preparation method and application thereof. The whitening composition is prepared from the following components: a tea leaf extract, a lavender flower extract and water, wherein the tea leaf extract, the lavender flower extract and the water are added into the whitening composition. The whitening composition can interact and synergistically improve the autophagy ability of cells, synergistically improve the phagocytosis of melanin in keratinocytes and the removal ability of skin metabolic wastes, can synergistically inhibit the generation of melanin and synergistically improve the expression of miR-137 genes in melanocytes, has a relatively high development prospect of cosmetics with whitening effects, and has a wide application prospect. Moreover, the composition is mild in property, easy to absorb and non-irritant, and can be widely applied to skin care cosmetics.

The utility model discloses a hemodialysis filtration treatment device, which relates to the technical field of hemodialysis filtration and comprises a base, a rotating wheel is fixedly mounted at the bottom of the base, a dialysate box is fixedly mounted on the upper surface of the base, a dialysis machine main body is fixedly mounted on the upper surface of the base, and a dialysis pump is rotatably mounted on one side of the dialysis machine main body. A control screen is fixedly installed at the top of the dialysis machine body, a liquid outlet pipe is fixedly installed on the dialysis machine body, a supporting rod is fixedly installed on the upper surface of the base, a liquid storage box is arranged on the upper surface of the base, a handle is fixedly installed on the liquid storage box, and a filtering mechanism is arranged at the other end of the supporting rod. Through the structural arrangement of the filtering mechanism and the fixing and adjusting mechanism, filtering holes in the filtering box can be prevented from being blocked, meanwhile, metabolic waste and waste liquid can be treated respectively, the treatment efficiency is improved, the dialyzer can be fixed, the height of the dialyzer can be adjusted, and the dialyzer can be prevented from falling off and can be adjusted to the height suitable for the dialyzer.

Disclosed in the present invention are a peripheral nerve catheter and a preparation method therefor. The peripheral nerve catheter comprises: an outer sheath, which is a hollow braided tube that has micron-sized pores and is non-wound; at least one inner core tube, which is placed in the outer sheath and is coaxially arranged with the outer sheath, each inner core tube being a hollow fiber tube that has micro / nano-sized pores and is non-wound; and filling gel, which is filled between the outer sheath and the inner core tube to fix the inner core tube. The bionic nerve catheter having a structure composed of the inner core and the outer sheath according to the present invention can achieve effective nerve regeneration, the inner core tube, which axially runs through the outer sheath, has a topological structure that is conducive to the adhesion and directional growth of nerve cells, and also has a micro / nano-sized pore structure that is conducive to the penetration of nutrients for growth and the formation of nerve connections and neurovascular network. In addition, the filling and curing of the filling gel effectively prevents the infection of damaged tissues, and is conducive to the penetration of nutrients and the transfer of cellular metabolic waste.

The invention relates to a brightening composition for improving skin transparency and glossiness and application of the brightening composition, and belongs to the technical field of cosmetics. The brightening composition comprises oxyresveratrol, a loquat leaf extract, a burdock seed extract and a verbascos extract, the composition can effectively resist oxidation and inflammation through the oxyresveratrol so as to relieve skin darkness, and the loquat leaf extract can promote cell autophagy so as to remove metabolic waste and improve skin permeability. The burdock seed extract improves the epidermis structure so as to enhance the skin light transmission, and the verbascos extract plays a role in instant brightening through a quantum transition effect. According to the composition, through the synergistic effect of all the components, the skin transparency and glossiness are remarkably improved, and the composition is mild, free of irritation and suitable for being used by people with sensitive skin.

Serum albumin is an important molecule in human and animal blood. It provides oncotic pressure and acts as a transportation agent, delivering nutrients from the blood to the tissue and removing waste products from the tissue to the blood. This invention describes an organ preservation and / or perfusion solution which comprises acetylated serum albumin, as well as method of making and using such a solution, and uses of acetylated serum albumin itself. The acetylated serum albumin utilised in the present invention can protect the vascular endothelium during perfusion of an isolated organ or tissue.

The invention relates to the technical field of cell culture, in particular to a large-scale cell culture device. Comprising a box body; the box door is rotationally connected to the side face of the box body; the controller is mounted on the side surface of the box body; the placing frame is placed in the box body; the plurality of culture boxes are arranged, and the plurality of culture boxes are placed on the placing rack in a vertically stacked manner; the liquid inlet pipe is connected to the bottom of the placement frame, and the end, away from the placement frame, of the liquid inlet pipe penetrates out of the side face of the box body; the liquid outlet pipe is connected to the side, away from the liquid inlet pipe, of the bottom of the containing frame. According to the invention, the public liquid inlet pipeline and the public liquid outlet pipeline which are respectively formed by connecting the first vertical pipes and the second vertical pipes of the plurality of culture boxes in series are arranged and are matched with the on-off assembly and the liquid level detector, so that automatic and quantitative addition of culture solutions in the culture boxes and centralized discharge of metabolic wastes can be realized; according to the design, the complexity of traditional manual one-by-one operation can be avoided, manpower is greatly saved, and the pollution risk can be effectively reduced by reducing the number of uncovering times.

The invention belongs to the technical field of cell culture, and particularly relates to an umbilical cord blood NK cell culture dish and a use method thereof. The culture dish is reasonable in design, the bone marrow microenvironment is simulated through the three-dimensional micro-scaffold, and the adhesion and proliferation efficiency of cord blood NK precursor cells is remarkably improved by combining biocompatible nanofibers (PLGA) and extracellular matrix proteins (fibronectin and heparin sulfate), so that gt; the amplification multiple is 1 * 10 < 4 >. The dynamic microfluidic gradient channel continuously provides cell factors (such as IL-15 and IL-12) and nutrition, maintains stable glucose concentration (gt, 3mM) and low lactic acid level (lt, 15mM), and prevents metabolic waste from inhibiting amplification. According to the invention, an anti-CD16 antibody and an NKG2D ligand (ULBP2) are fixed on the surface, ADCC effect and tumor targeted killing ability of NK cells are enhanced through a costimulatory signal (CD137L), and cytotoxicity (K562 killing rate) gt is enhanced; 80%.

The application discloses a brain hemorrhage prognosis improvement method based on intestinal flora regulation and relates to the technical field of intestinal microecology.The application comprises the following steps: detecting feces, starting intervention if the enterococcus abundance and neuroinflammatory metabolic markers exceed the standard, stopping the use of conventional nutrient solution, perfusing a special component containing sugar analogs, blocking the energy uptake of enterococcus, simultaneously providing a carbon source for beneficial bacteria, synchronously perfusing a microsphere suspension containing a molecular capture function, adsorbing and fixing proinflammatory metabolic waste in the intestinal tract, blocking the blood entry of the proinflammatory metabolic waste, perfusing live bacteria with different functions three times in time sequence, sequentially completing the acidification of the environment, constructing a bacterial membrane barrier and repairing the intestinal epithelium, and re-detecting after each round of intervention, selecting the maintenance period or adjusting the intensity and then recycling according to the decrease of enterococcus and markers.The application can limit the competitive advantage of enterococcus in the utilization of carbon sources after brain hemorrhage by sequentially implementing competitive inhibition of the phosphotransferase system and multi-species ecological niche occupation.

The present application provides a kind of composite hydrogel for scar repair and its preparation method, including photo-crosslinking hyaluronic acid methacrylate and layered silicate nanocrosslinking agent dispersed in photo-crosslinking hyaluronic acid methacrylate, wherein photo-crosslinking hyaluronic acid methacrylate is physically adsorbed with layered silicate nanocrosslinking agent to form a double crosslinking network structure.The present application can be firmly attached to the wound to prevent bacterial invasion, and can also release the long-acting, controllable slow release of load factor, extend the local treatment window, improve bioavailability, and through the double crosslinking structure, it can also simulate extracellular matrix, facilitate the transport of oxygen, nutrients and cell metabolic waste, greatly improve the scar repair effect.

The invention discloses application of plasmalogen in assisting in gaining muscle and reducing fat in aerobic exercise and relieving skeletal muscle and kidney injury caused by exercise, and belongs to the technical field of biological medicine. According to the invention, a model of mouse skeletal muscle and kidney injury caused by exhaustion movement is established in vivo, and skeletal muscle and kidney injury is relieved by intervention of a sea squirt phospholipid extract and sea squirt plasmalogen respectively. Skeletal muscle injury and metabolic waste entering blood of a C57BL / 6J mouse caused by continuous exhaustion exercise for 7 days greatly increase the kidney burden. The sea squirt phospholipid extract and the sea squirt plasmalogen relieve muscle and kidney injury caused by exhaustive exercise and improve exercise performance.

The invention discloses a nephrology department pressurization ultrafiltration device which comprises a blood filter press used for conducting pressurization ultrafiltration on blood, two peristaltic pumps are arranged on the outer surface of the blood filter press, a semi-permeable membrane filter is arranged in the blood filter press, and an anti-blocking assembly is arranged in the semi-permeable membrane filter. Through the synergistic effect of the trapezoidal structure of the tubular semi-permeable membrane, the spiral flow guide design and flexible stirring, the blood is kept at a stable flow speed in the filtering process, the blood backflow capacity difference caused by blockage is avoided, the problem that concentrated metabolic waste is sucked back to a blood vessel again after ultrafiltration is completed is solved, and the filtering efficiency is improved. The residual quantity of metabolic wastes in the blood after ultrafiltration is reduced, and the filtering effect is improved.

The invention belongs to the technical field of aquatic product transportation. More specifically, the invention relates to a product and a method for improving aquatic product intensive transportation survival rate and reducing stress response. According to the composition for aquatic product transportation provided by the invention, the composition is added into a transportation water body, and a pretreatment measure is matched, so that the transportation effect is remarkably improved. Tests prove that the stress behaviors of the fishes transported for a long distance by adopting the method are greatly reduced, and the vigor of survival individuals is high. And preferably, after an adsorbent zeolite powder is introduced, the accumulation of metabolic wastes such as ammonia nitrogen can be effectively delayed, and the survival rate of the fish is further improved. The transportation method is easy and convenient to operate and environmentally friendly, can be seamlessly matched with existing aquatic product transportation equipment, does not need additional transformation or midway water change, has good universality and generalizability, is suitable for various aquatic products and different transportation distances and scenes, and has good application prospects and value.

The application discloses a safe, efficient and low-cost cleaning solution for suspended cells and application thereof, which comprises multiple kinds of glucose, amino acid supplements, vitamin supplements, anti-shear protective agents and sodium bicarbonate, is designed for the harvesting and washing process of suspended cells, can effectively remove residual culture medium, cell fragments, metabolic waste and process-related impurities while effectively maintaining cell viability, viable cell density and normal morphology, so as to realize the harvesting target of high-purity, high-activity and low-risk material of suspended cells, and is especially suitable for the production process of high-demand cell products such as cell culture meat. The cleaning solution system can be adapted to the continuous harvesting process of a large-scale bioreactor, can be integrated with automatic harvesting equipment, realizes stable, efficient and repeatable cell collection and washing operation, and meets the full-scene requirements from laboratory research and development to industrial production.

The invention discloses an intelligent bioreactor system based on multi-parameter real-time monitoring and feedback control, and belongs to the technical field of bioengineering. The system comprises a bioreactor main body, an integrated biochemical analyzer and a feedback control system, and real-time sampling of a culture solution is realized through a bypass circulating system. The integrated biochemical analyzer comprises an optical detection module, an electrochemical sensor module and an overflow pool biochemical detection module, and can detect the OD value, pH, NH4 < + >, K < + > plasma concentration and the concentration of at least three metabolites such as glucose, lactic acid and glutamine in parallel. And the feedback control system dynamically adjusts the feeding strategy, the pH and the gas flow based on a metabolite concentration change rate prediction model and a multi-level control algorithm. The system can accurately maintain the culture environment, inhibit the accumulation of metabolic wastes, improve the nutrient utilization efficiency, obviously enhance the process stability and the production efficiency, and provide an intelligent accurate regulation and control solution for cell culture and microbial fermentation.

Improved methods for large scale production of proteins and / or polypeptides in cell culture is provided. In accordance with the present invention, the method provides for culturing cells that have metabolically shifted. The use of such a method or system allows high levels of protein or polypeptide production and reduces accumulation of unwanted metabolic waste such as lactate. Proteins and polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, immunogenic, or other commercial biologic compositions, such as antibodies.

The invention discloses a lotus leaf, carbon and poria cocos synergistic medicinal and edible chewable pill and a preparation process thereof. In the invention, the synergism of the lotus leaf charcoal and the poria cocos is the core advantage of the product. After the lotus leaf charcoal is calcined and carbonized, the porous structure of the lotus leaf charcoal enhances the adsorption capacity and can assist in adsorbing excessive moisture and metabolic wastes in the body; poria cocos has the effects of tonifying spleen and excreting dampness, and normal delivery of water is promoted by regulating spleen and stomach functions. The adsorbability of the lotus leaf charcoal helps to reduce retention of water-dampness, and the spleen-tonifying effect of the poria cocos can fundamentally improve unbalance of water metabolism and jointly strengthen the dampness-clearing effect. The synergism not only improves the efficacy of a single component, but also avoids the problem of impairment of healthy qi possibly caused by pure diuresis, so that the damp clearing process is milder and durable, the compatibility of multiple medicinal and edible materials in the whole formula realizes multi-dimensional complementation of functions, and the rose flowers are matched for soothing the liver and regulating qi, so that unsmooth mood caused by encumberment of spleen due to water dampness can be improved. The liquorice can relieve urgency and regulate the middle warmer, so that the palatability and the safety of taking are further improved.

The invention provides a method and device for determining the fishery breeding density of a wind power plant area and a storage medium. The method comprises the steps that planning parameters of a wind turbine generator, planning parameters of fishery breeding, historical tide data and the fishery breeding variety of a sea area where a wind power plant construction area is located are obtained; according to the planning parameters of the wind turbine generator, the planning parameters of fishery breeding and historical tidal current data, a three-dimensional fluid dynamic model is constructed, and the seawater flow velocity and direction of the fishery breeding area are determined; the method comprises the following steps: constructing a seawater dissolved oxygen diffusion model and a fish metabolic waste concentration model according to a fishery breeding variety and seawater flow velocity and direction, and determining the distribution of dissolved oxygen in seawater and the concentration of fish metabolic waste; and according to the distribution of dissolved oxygen in seawater and the concentration of fish metabolic wastes, determining the breeding density of the fishery breeding varieties in the fishery breeding area.

The invention provides a costal cartilage stent and a manufacturing method, and the costal cartilage stent comprises a defect part which is the same as the defect part of the costal cartilage in shape; the connecting part is connected with the defect part and located at the end of the defect part, the connecting part is provided with a connecting hole, and the connecting hole is used for being connected with a non-defect part of the thorax; wherein the defect part is composed of continuously-arranged hole-shaped structures, and each hole-shaped structure is formed by a plurality of beam rods in a surrounding mode; the porosity of the porous structure is adjusted by adjusting the diameter of the beam rod and / or adjusting the distribution of holes of the porous structure, the porosity is A, and A is equal to 30%-70%. Therefore, the mechanical strength of different areas of the stent can be close to the mechanical strength of different areas of costal cartilage of a human body, and the stent has better tissue growth environment, nutrient substance and metabolic waste exchange capacity after being implanted into the human body, so that the stent is more suitable for cell retention.

The invention provides a real-time detection and dynamic feeding system and method for an NK cell suspension culture nutrient solution. The system comprises a multi-parameter sensing module used for monitoring the concentration of multiple components in a culture solution in real time; the intelligent decision control module is used for dynamically predicting nutritional requirements and generating a feeding strategy according to the multi-parameter data monitored in real time and based on related models and algorithms; the precise feed supplement execution module is used for injecting a nutrient solution and a pH adjusting solution as required through a multi-channel peristaltic pump according to the generated feed supplement strategy, supplementing growth factors at regular time, and is provided with a metabolic waste removal unit for removing lactic acid and ammonia metabolic waste; and the feedback optimization database is used for recording culture process data, optimizing a feeding strategy through a machine learning algorithm, and adapting to individual requirements of different cell strains. The technical problems that in the prior art, a static feeding strategy cannot respond to nutrition dynamic changes in real time, metabolic byproducts lack a real-time removal mechanism, and the cell quality is unstable due to nutrition imbalance are solved.

The invention discloses a construction method of an in-vitro full-thickness skin model based on a dynamic skin chip, belongs to the technical field of biology, and particularly relates to the construction method of the in-vitro full-thickness skin model based on the dynamic skin chip. The construction method comprises the following steps: injecting a fibroblast suspension and a 3D epithelial cell culture medium into a dynamic skin chip for culturing, and constructing to obtain a corium layer; and inoculating keratinocytes above the corium layer for differentiation culture, and carrying out dynamic differentiation treatment to obtain the in-vitro full-thickness skin model. On the basis of the dynamic skin chip, a nutrient perfusion and metabolic waste removal approach is provided by simulating the structure and the function of an in-vivo blood circulation system, the complete in-vitro full-thickness skin model with the physiological function is constructed and formed, and the model can simulate the permeation process and the metabolic process of a medicine in the human skin, so that the in-vitro full-thickness skin model has a good application prospect. Therefore, an effective platform is provided for accurately evaluating the curative effect and the safety of the medicine.

The application discloses a method for designing a bionic femur support based on lattice structure and TPMS structure fusion, and belongs to the technical field of bionic bone support. The application respectively creates a CAD model of a Diamond unit homogeneous porous structure and a CAD model of an Iso Truss unit homogeneous porous structure; a Ramp fusion function is created to fuse the Diamond unit homogeneous porous structure and the Iso Truss unit homogeneous porous structure to generate a preliminary ITD fusion CAD model; then the preliminary ITD fusion CAD model and a newly established cylinder III are fused by using a Boolean intersection instruction to form a final ITD fusion CAD model, finally, the final ITD fusion CAD model is divided into a finite element simulation grid model and an inp format simulation model file is output; the inp format simulation model file is imported into HyperMesh software to repair and divide a grid and an inp file format model is exported. The bionic bone support designed by the application has higher bearing capacity and stability, forms a mechanical property gradient, and is suitable for complex mechanical environments. Meanwhile, the bionic bone support can promote cell adhesion, proliferation and differentiation, transport nutrients, discharge metabolic waste, and improve cell survival rate.

The utility model relates to the technical field of semen storage, in particular to a boar semen constant-temperature storage device which comprises a constant-temperature box body, a box cover is rotatably connected to the top of the constant-temperature box body, a control panel is arranged on the side wall of the constant-temperature box body, sliding grooves are formed in the two sides of the inner wall of the constant-temperature box body, and sliding plates are slidably connected into the sliding grooves. A supporting plate is fixedly connected between the opposite faces of the sliding plates, a shaking mechanism used for shaking a test tube storing boar semen is arranged at the top of the supporting plate, and the shaking mechanism comprises supporting frames fixedly connected to the two sides of the top of the supporting plate. According to the method, accumulation of metabolic waste and gathering and adhesion of sperms in a standing state are effectively reduced, so that sperm decline is delayed, a more stable and more efficient condition is provided for long-term preservation of the boar semen, long-term high-quality semen preservation is facilitated, and the utilization rate and effectiveness of the semen are improved.

The utility model discloses a stem cell in-vitro dynamic scale amplification device which comprises a box body, the front end of the box body is rotatably connected with a box door, the top of the front end of the box door is fixedly connected with a control screen, the bottom of the rear end of the box body is fixedly connected with two fans, the bottom end of the interior of the box body is fixedly connected with a waste liquid collecting box, and the waste liquid collecting box is fixedly connected with a liquid outlet. A culture environment control system is installed in the box body and comprises a gas environment control module and a temperature environment control module, a plurality of placing plates are fixedly installed on the left side and the right side of the inner wall of the box body, a material placing cavity is formed between the placing plates, and a plurality of layers of culture plates are slidably connected into the material placing cavity; according to the utility model, the stability of a culture environment, the dynamic nature of a culture solution and the initiative of gas exchange are realized, so that cells can obtain better gas exchange, nutrient supplementation and timely discharge of metabolic wastes in the whole culture process, and the cell culture device can quickly adapt to a culture process based on GMP (Good Manufacturing Practice) requirements; and more and healthier culture results can be obtained in a short time.

The invention relates to the technical field of health food, and particularly discloses a tea leaf theanine composition with an anti-fatigue effect and a preparation method thereof, and the tea leaf theanine composition comprises the following components: tea leaf theanine, gamma-aminobutyric acid, a plant extract, coenzyme Q10, taurine, compound B vitamins, a natural antioxidant and a functional carrier. The preparation method of the tea leaf theanine composition comprises the following steps: (1) preparing the composite plant extract; (2) preparing embedding powder; and (3) mixing the compound plant extract, the embedding powder, the tea leaf theanine, gamma-aminobutyric acid, taurine, compound B vitamins and a natural antioxidant, adding an adhesive, carrying out wet granulation, drying, granulating, and screening to obtain the tea leaf theanine composition with the anti-fatigue effect. The tea leaf theanine composition prepared by the invention shows remarkable anti-fatigue activity, and can realize an anti-fatigue effect by increasing energy reserve, reducing metabolic waste accumulation and enhancing antioxidant capacity.

The invention relates to a sorbent for removing metabolic waste products from a dialysis liquid, the sorbent comprising a soluble source of sodium ions. The sorbent comprises an ion exchange system which converts urea to ammonium ions and which is configured to exchange ammonium ions for predominantly hydrogen ions and to exchange Ca, Mg, and K for predominantly sodium ions. The soluble source of sodium ions overcomes an initial drop in sodium concentration in regenerated dialysate. When used in conjunction with an infusion system configured to utilise exchange of Ca, Mg and K for sodium during dialysate regeneration a desired sodium ion concentration can be maintained.

The application discloses an in-vitro culture and separation method of NK cell exosome loaded with PD-1 antibody, relates to the field of biomedical technology, and specifically comprises the following steps: (1) NK cell culture; (2) PD-1 antibody loading; (3) exosome extraction; (4) exosome purification; efficient loading of the PD-1 antibody is realized through electroporation, high expression of the antibody in the exosome is ensured, optimized NK cell culture conditions and exosome extraction procedures guarantee large-scale production of the exosome; by adding inorganic particles as microcarriers in cell culture, more attachment points are provided for cells, transfer of nutrients and removal of metabolic waste are optimized, the cell proliferation fold is improved, the cell survival rate is maintained, the overall loading efficiency and growth amount of the microcarriers are improved, thereby the growth and proliferation of the NK cells are promoted, and large-scale culture of the cells is realized.