65 results about "Chaetoceros" patented technology

The invention provides an automatic chaetoceros and non-chaetoceros sorting method based on microscopic images. The automatic chaetoceros and the non-chaetoceros sorting method based on the microscopic images includes the steps: (1) extracting binarization images of target cells of the algae from the microscopic images of the algae; (2) setting the binarization image height, namely the line number as three hundred pixels regularly, and zooming the rows according to the original proportion; (3) performing closing operation for the images by utilizing a round structure element with a radius of five pixel width; (4) extracting skeleton from the binarization images by utilizing a distance transforming refinement algorithm; (5) extracting node number and endpoint number: extracting node assembly and endpoint assembly of the skeleton, and then acquiring the node number and the endpoint number; (6) judging whether the skeleton is the chaetoceros or not according to the criterion whether the requirements that the node number is not less than 5 and the endpoint number is not less than 5 are met or not. The automatic chaetoceros and the non-chaetoceros sorting method based on the microscopic images is high in accuracy, clear in development, very obvious in economic benefit and social benefit, lays a base for further algae sorting recognition, improves further recognition ratio for the automatic sorting of the algae, and can provide effective instructions and assistance for various algae monitoring personnel and researchers in the front line.

The invention relates to a high-density culture process of autotrophic oil-producing microalgae. A fermentation method combining a partition method and a two-step method is adopted, CO2 waste gas discharged from a thermal power plant is used as a carbon source, municipal sewage after living contaminant or miscellaneous algae filtering removal is used as a culture medium, the autotrophic oil-producing microalgae is cultured in a high-density way for growth, perfectly, sea salt is added into the municipal sewage for simulating sea water so that the permeation pressure is increased, the living contaminant or miscellaneous algae pollution is prevented, and the autotrophic oil-producing microalgae is selected from one kind or several kinds of materials from autotrophic chlorella, nannochloropsis salina, botryococcus braunii, chaetoceros gracile, marine green algae, isochrysis galbana, nitzschia closterium, phaeodactylum tricornutum, dunaliella and prism-shaped algae. When the high-density culture process is adopted, high removal efficiency of nitrogen, phosphorus and CO2 can be realized, in addition, the algae concentration, the grease content and the protein content are high, the double effects of environment protection and biological resources production are reached, the operation is simple, convenient and fast, the cost is low, no pollution is caused, social and economic integrated benefits are high, and the high-density culture process is suitable for large-scale popularization and application.

The invention discloses an environment-friendly and high-yield breeding method for penaeus vannamei boone. The method is characterized by comprising the steps of previous-breeding preparation, culture of chaetoceros, adding of a carbon source, feeding and management, water quality monitoring and the like; before breeding, favorable chaetoceros is added to occupy an ecological niche; during breeding, the input ratio of feed to glucose is regulated, heterotrophic bacteria can be stimulated to absorb nitrogen and phosphorus metabolic waste for mass propagation, and then the function of water purification is achieved; by the adoption of a special siphon dirt collection device with an excellent dirt discharge effect, the method enables the water change frequency to be reduced to one time every seven days, and the water change volume is only 5%. The method has the advantages of being simple and controllable, remarkably reducing the feed conversion rate and water change cost and greatly increasing the yield and survival rate of the penaeus vannamei boone, thereby having good biological and economic benefits.

A method for artificial oxytocin and fertilization of Mytilus coruscus comprises the following steps of a parent cultivating pool: building an outdoor cultivating cement pool with the dimension of 10cmx1.5mx1m, stocking inflatable stones, covering canvas above the cement pool to prevent rain and sunlight, and shading the periphery of the cement pool by dark cloth to regulate strength of light; selecting Mytilus coruscus which has new and integral shell surfaces, full gonads, complete gill, light red ovaries and white testes, and is free of damage and powerful in closure of double shells; preparing baits, wherein chlamydomonas live baits including cultured Dunaliella viridis, latymonas subcordiformis and Chaetoceros serve as primary baits, and artificial compound feed including spirulina powder, chlorella powder and yeast tablets serves as secondary baits; drying parent Mytilus coruscus with developed gonads in the shade for 6-8 hours, stimulating the parent parent Mytilus coruscus for 1 hour through running water, and feeding the parent parent Mytilus coruscus into prepared warming seawater.

A liquid for culturing diatom comprising nitrogen, silicon and phosphorus, wherein a value of silicon/nitrogen, representing the ratio of concentration by mass of silicon to nitrogen, is set to be a value of 0.06 or more, and a value of phosphorus/nitrogen, representing the ratio of concentration by mass of phosphorus to nitrogen, is set to be a value of 0.18 or more, and the pH of the culture liquid is set in the range of 6.4 to 8.4. The culture liquid allows the culture of Chaetoceros at a cell concentration greater than the maximum value (3 X 10<13> cells/m<3>) achieved by the conventional culture methods and with stability.

The invention relates to the technical field of aquaculture, in particular to an environment-friendly method for rearing prawn by utilizing chaetoceros. The method comprises the following steps of: rearing prawn larvae in a rearing pool; and adding nutrients and chaetoceros needed by the larvae at each rearing stage in the whole larva rearing process until the larvae grow up, wherein 0.2-1.2 million/ml of chaetoceros are kept in the rearing pool. On the basis of rearing the prawn larvae by effectively utilizing an ecological water environment mainly containing the chaetoceros, the invention realizes the zero pollution of the ecological environment by only adding water without draining, does not use any antibiotic and truly realizes environment-friendly and healthy production of the prawn larvae.

The invention belongs to the field of microalgae cultivation, and relates to a culture medium formula and a full-artificial quick cultural method of Chaetoceros mulleri. The culture medium formula comprises 0.1-0.3g/L of urea, 0.05-0.1g/L of sodium hydrogen carbonate, 0.1-0.5g/L of sodium silicate hydrate crystals, 0.01-0.02g/L of potassium dihydrogen phosphate, 3-5g/L of EDTA (Ethylene Diamine Tetraacetic Acid), 1-5mg/L of VB1 and 1.0-3 microgram/L of VB12, wherein the solvent of the formula is seawater. The cultural method comprises the following steps: after disinfecting a culture tank and seawater, preparing a culture medium according to the formula by using seawater as the solvent; inoculating the algae to the culture tank filled by the culture medium; cultivating after sealing; illuminating by light with three primary colors and inflating air which is filtered by an air filter element with the aperture which is less than 0.1 micron during cultivation; and cultivating for 3-5 days and collecting. The culture medium formula provided by the invention is balanced in nutrition and low in cost; according to the cultural method, the chaetoceros mulleri grows quickly and the method is simple in process operation.

The invention discloses a breeding method of a glyptocidaris crenularis offspring seed, comprising the following steps of: cultivating chaetoceros in a high temperature season, centrifugally concentrating and then storing at low temperature; beginning to cultivate benthic diatoms 10-20 days before collecting seedling of glyptocidaris crenularis, and selecting an individual with a shell diameter of at least 8 cm, health and no damage and favorable gonadal development at 2-3 ages as an affinity choline; inducing the affinity choline to lay eggs by using a KCl solution stimulus method, artificially inseminating, then hatching the germ cells in ponds according to the density of 3-5 per ml, micro-aerating in the hatching period and timing-stirring, wherein the hatching water temperature is 15-18 DEG C; selecting healthy larvae at the upper layer, breeding according to the density of 0.4-0.6 per ml, feeding the chaetoceros stored at low temperature in the breeding period; placing the grown infantile choline into a seedling-collecting pond internally provided with a corrugated plate where the benthic diatoms grow, and breeding the choline to reach the diameter of 3-5 mm; and peeling the infantile choline from the corrugated plate to be cultivated, and cultivating the infantile choline to grow into a young choline with the diameter of more than 1 cm, at this moment, the young choline can be used for artificial culture as the offspring seed.

The invention discloses a standardized breeding method for young holothuria leucospilota. Standardized management is carried out on each key step in a breeding process of young holothuria leucospilota, including water quality control, pest and disease organism prevention and control, unicellular algae bait cultivation, artificial spawning induction, youngholothuria leucospilota feeding and the like, such that stability and high yield of young holothuria leucospilota are ensured. Water for breeding is filtered by a microporous filter bag, such that tiny particles such as eggs in seawater can be further removed and outbreak of harmful organisms is completely eradicated from the source. A full-artificial environment is used for culturing unicellular algae. Chaetoceros in the culture method does not depend on natural illumination, can continuously and rapidly grow all day long, can be prevented from being damaged by wind, rain, high temperature and the like in outdoor culture, can also be prevented from being harmed by protozoa and other enemies, can stably provide fresh and live unicellular algae baits for young holothuria leucospilota, and does not introduce diseases/enemies even if being directly fed.

The invention discloses a bostrychus sinensis feeding method and an acoustic domestication feeding system thereof. The method is characterized in that excellent diatom is inoculated after water changing, chaetoceros is selected as the diatom, the feed feeding is decreased progressively according to the levels of 8%, 7%, 6%, 5% and 4% of the total weight of fish, each level is fed for 3 days, 15 days serve as a period, after 15 days, 100% water change is carried out, a fishing pond is cleaned, weighing is carried out, the above operation is repeated, an acoustic domestication feeding system isadopted for feeding during feeding, the acoustic domestication feeding system comprises a support frame, and a hydrophone and a camera which are positioned in a culture pond, four feeding machines, aloudspeaker and a controller are arranged on the support frame, and the feeding machines, the loudspeaker, the hydrophone and the camera are all electrically connected with the controller. The methodhas the functions of improving the feeding efficiency, reducing the feed loss, adjusting the pH value of the water body and inhibiting the growth of bacteria, so that the fatality rate of the bostrychus sinensis is reduced, and the survival rate of the bostrychus sinensis is increased.

The invention discloses a macro-element nutrient salt culture medium for large-scale culture of Chaetoceros mulleri and a preparation method thereof. The macro-element formula culture medium is prepared by using mother liquor A and mother liquor B through mixing according to volume ratio 1:1, wherein the mother liquor A is mother liquor A (1000x) consisting of 20 to 25 parts of ammonium hydrogen carbonate by weight, 1 to 2 parts of sodium dihydrogen phosphate by weight, 0.33 part of ferric trichloride by weight, 1.64 parts of EDTA-2Na and balance of water; the mother liquor B consists of 15 to 20 parts of sodium silicate by weight and 1000 parts of water by weight. By adopting the macro-element nutrient salt formula provided by the invention to culture the Chaetoceros mulleri, the growth of the Chaetoceros mulleri can be promoted, the culture time is prolonged, the high-density culture of the Chaetoceros mulleri is realized and the culture cost is reduced.

The invention discloses a method for industrially and ecologically breeding litopenaeus vannamei shrimps in indoor space. The method includes an industrially and ecologically indoor breeding process and a management method of the litopenaeus vannamei shrimps. According to the method, a traditional shrimp breeding technique is innovated, healthiest young litopenaeus vannamei is selected, the shrimps are stocked by the aid of the most reasonable density and fed by the aid of diatoms such as micro-thalassiosira rotula and chaetoceros mulleri and high-quality natural organism baits such as fairy shrimp nauplii, part of artificial feed and baits are replaced, nutrition can be enhanced, immunity can be improved, water quality change of a shrimp pond can be effectively controlled, self-cleaning capacity of water is enhanced, growth of harmful bacteria is restrained by the aid of beneficial bacteria such as lactic acid bacteria, bacillus and pseudomonades, residual feed, shrimp remains and thelike are decomposed, and a stable ecological system of the shrimp breeding pond is built. According to the method, healthy, rapidly-grown, high-stress-resistant and high-quality shrimps can be bred,shrimp breeding survive rate can be increased, shrimp breeding cost is saved, and the shrimp quantity requirements of clients are met, so that economic benefits are improved.

the invention discloses a penaeus vannamei breeding method. The method comprises the following steps: firstly, injecting seawater into a disinfected pond, wherein the pond communicates with a water circulation device; before throwing young penaeus vannamei, performing inoculation by using 10-15 million/mL of chaetoceros and 10-15 million/mL of dunaliella salina, wherein the chaetoceros and the dunaliella salina are cultivated by using a nutrient solution, and the nutrient solution comprises the following raw materials: ferric chloride hexahydrate, ethylenediamine tetraacetic acid disodium salt, copper sulfate pentahydrate, sodium molybdate dihydrate, zinc sulfate heptahydrate, cobalt chloride hexahydrate, manganese chloride tetrahydrate, vitamin B1, vitamin H, vitamin B12, vitamin B6 and sea salt; after the water quality is stable, throwing 450-550/m<2> of shrimp fry into the pond; feeding 50-500 grams of feed for every ten thousand of shrimps, wherein 15-20% of the shrimp feed is directly eaten by the shrimps, and the balance of the feed is decomposed by a water treatment device to serve as the nutrition of the chaetoceros and dunaliella salina; meanwhile, keeping the density of the chaetoceros and the dunaliella salina in the pond to be 10-15 million/mL; and performing harvesting in 40-70 days after the shrimp fry are thrown. The penaeus vannamei breeding method provided by the invention is high in breeding survival rate, large in prawn specification and large in yield.

The invention provides an artificial breeding method of giant trevally. The artificial breeding method of giant trevally comprises the following steps that healthy female and male giant trevally withthe age more than three are selected as parents; net cage breeding is selected, and the female and male giant trevally are fed with fodder A and fodder B every day; when sexual maturity of parent fishis achieved, natural spawning and fertilization are carried out, fertilized ovum is fished out and placed into a breeding pool for hatching, pool water is inflated slightly, and larva fish is obtained; 10000-12000 thousand/mL dunaliella salina, 3000-6000 thousand/mL chaetoceros and 7000-9000 thousand/mL chlorella are inoculated, and the larva fish is bred; and the female and male giant trevally are fed with fodder C every day five days later, and fish fry is obtained after breeding for 20-30 days. By adopting the artificial breeding method of giant trevally, the oviposition quantity of the parent fish is high, the fertilization rate can reach more than 98%, the hatching rate of the fertilized ovum reaches more than 94%, the quality of the fertilized ovum is high, the survival rate of breeding the larva fish is high, the fish fry yield of the giant trevally is large, the breeding efficiency is high, and the breeding quality is good.

The invention relates to a PCR (Polymerase Chain Reaction) primer group for distinguishing five types of shellfish bait microalgae, and the PCR primer group is used for identifying Chaetoceros of Bacillariophyta, Isochrysis galbana and Pavlova viridis of Chrysophyta and Platymonas helgolandica and Nannochloris oculata of Chlorophyta, and nucleotide sequences of primers are respectively SEQ ID NO: 1-10. According to the invention, specificity primers of five types of bait microalgae can be provided, a PCR technology is combined to carry out in vitro amplification for 18S r DNA (Deoxyribonucleic Acid) of microalgae, and an amplification result can be analyzed through an AGE (Agarose Gel Electrophoresis) technology. Through the amplification result, the primers and templates can be better combined, and an amplification reaction can be triggered, so that whether the five types of shellfish bait microalgae exist in a sample or not can be quickly sensitively distinguished, detected and identified.

The invention belongs to the field of aquaculture, and particularly relates to a cultivation method for tripnenstes gratilla larvae. Incubation equipment is manually installed, and incubation conditions are controlled, and living concentrated chaetoceros is taken as delivered feed, and meanwhile, the water quality of an incubation water body is kept by delivering bacillus licheniformis and effective microorganisms (EM), so that the success rate of incubation of the tripnenstes gratilla larvae can be obviously improved, and the incubation rate can reach 0.4% or more. According to the cultivation method for tripnenstes gratilla larvae disclosed by the invention, a new superior variety is provided for development of tropical sea urchin culture in China, and development of tropical sea urchinculture industry in China can be effectively promoted; abundant cultured larvae are provided for ocean pastures of coastal sea areas in Hainan province and Sansha sea areas, and new employment post and ways are provided for fishermen to transfer jobs; and the cultivation method has very important economic value and social value.

The invention discloses a compound feed for breeding larvae of tropical sea cucumbers in a planktonic period and a preparation method of the compound feed. The preparation method of the compound feed comprises the following steps of mixing 8-12 g of gulfweed powder, 150-250 mL of chilled gulfweed liquid, 1-3 g of stable diatom species, 1-3 g of chlorella powder and 1-3 g of yeast powder in 5 L of clean seawater, putting a mixture into a 500-mesh net bag, filtering, and taking a filtrate, thereby obtaining the compound feed for breeding the larvae of the tropical sea cucumbers in the planktonic period. The compound feed for breeding the larvae of the tropical sea cucumbers in the planktonic period can completely replace natural bait chaetoceros, and the purposes of saving manpower and material resource cost and guaranteeing stable sea cucumber breeding work are achieved.

The invention discloses a breeding method for Pincushion Urchin. Compared with a traditional sea urchin breeding method, a large number of Pincushion Urchin larvae can be bred under the quite low energy consumption and culture wastewater discharge conditions so as to achieve the purposes of reducing natural resource fishing and catching pressure, and the problems that the transportation cost is high and the survival rate is low are solved. The method comprises the steps that firstly, a greenhouse is adopted as the breeding environment, under the effect of the greenhouse, water temperature of culture water is kept within the large range, no artificial heating seawater is adopted for breeding, and under the condition of saving the cost, pollution caused by a boiler to the environment is avoided; meanwhile, in the cultivation process, illumination within the range of 1,500-2,500 lx is adopted in the breeding process for culturing larvae, Chaetoceros mulleri and seawater photosynthetic bacteria are put, the purpose of purifying water can be achieved, and the problem that the cost is caused when a large amount of water is changed frequently is avoided.

The invention relates to a stichopus japonicus artificial breeding technology and relates to the field of trepang breeding. At present, researches and studies on artificial trepang breeding are few in China and even all over the world and systematized data on the artificial seedling technology are lacked. According to the stichopus japonicus artificial breeding technology, problems worth notification of all procedures are introduced in a systematized mode in the aspects of seedling facilities, water quality conditions, algae sort selection, bait cultivation and the like. Especially, caught and selected parent trepang should be scatheless trepang with the length of more than 20cm, the weight of more than 250g and the gonad index of more than 10%; larvas are bred, a shade-drying running water stimulation method is usually adopted in a breeding plant for inducing spawning, the cast bait is mainly cultivated algae includes chaetoceros mulleri, phaeodactylum, nitzschia closterium and the like. All procedures of spawning, incubation and larva breeding are well executed by controlling the cultivation density and the good effect is achieved.

The invention provides a culture method for rapid propagation of bait microalgae. The culture method comprises the following steps: adding an organic carbon source into an f/2 culture medium to culture the bait microalgae; wherein the bait microalgae are pseudominiature sea chain algae, chlorella or chaetoceros respectively; preferably, the organic carbon source added into the f/2 culture medium is glycerol, acetic acid or glucose, wherein the addition concentration of the organic carbon source is 0.5-5.0 g/L. According to the method, the nutritional value of the bait microalgae can be effectively improved, the propagation speed of the bait microalgae can be increased, the production cost of the bait microalgae is reduced, the economy of shellfish culture is improved, and technical supportis provided for wide and low-cost application of microalgae shellfish baits.

The invention relates to an artificial culture method for Onchidium struma larvae. The method comprises the following steps of: disinfecting a larva incubator and a culture box; disinfecting sponge bars; preparing disinfecting seawater; pouring the disinfecting seawater into the larva incubator and the culture box, aerating the disinfecting seawater in the larva incubator and the culture box by using an aerator, and controlling the water temperature and illumination intensity in the larva incubator and the culture box; putting fertilizer eggs of Onchidium struma into the larva incubator, and when checking to discover that Onchidium struma larvae are out of membranes in the larva incubator, putting the disinfected sponge bars; and squeezing the sponge bars to make water squeezed out of thesponge bars flow into water in the culture box together with the Onchidium struma larvae, and feeding the Onchidium struma larvae with chlorella vulgaris, isochrysis galbana and chaetoceros mulleri after the Onchidium struma larvae are transferred from the larva incubator into the culture box. The survival rate of the artificially cultured Onchidium struma larvae is 77.0-86.5 percent, and a requirement for large-scale production is met.

The invention discloses a feed for artificial breeding of bullacta exarata. The feed is prepared from, by weight, 21 to 25 parts of flour, 10 to 13 parts of soybean meal, 7 to 9 parts of corn flour, 5to 10 parts of squid powder, 8 to 12 parts of silkworm cocoon powder, 4 to 6 parts of shrimp shell powder, 3 to 8 liver powder, 25 to 28 isochrysis galbana, 17 to 22 parts of chaetoceros, 15 to 19 parts of euphorbia thymifolia, 2 to 9 parts of artemisia argyi, 1 to 6 parts of bidens pilosa, 11 to 15 parts of calcium dihydrogen phosphate, 7 to 13 parts of zeolite powder, 1 to 4 parts of montmorillonite, 1 to 4 parts of bentonite, 3 to 6 parts of choline chloride, 5 to 7 parts of betaine, 16-22 parts of percarbonamide, 6-10 parts of methyl cellulose and 7-9 parts of complex minerals. The feed can improve the survival rate of the artificial breeding of the bullacta exarata, shorten the breeding time of the bullacta exarata, and increase the yield of the bullacta exarata.