32results about How to "Cultivate stable" patented technology

The invention discloses a wall adherence/suspension type cell culture unit as well as a device, a system and a method thereof and relates to the technical field of cell culture devices. The culture unit comprises a shell, wherein a plurality of cell growth plates are arranged inside the shell; the adjacent cell growth plates are alternately connected to the left side wall or the right side wall of the shell; the front side surfaces of the cell growth plates are fixed to a front side plate of the shell, and the rear side surfaces of the cell growth plates are fixed to a rear side plate of the shell; a gap is retained between the adjacent cell growth plates; a roundabout one-way flow channel is formed among the cell growth plates and the inner wall of the shell; a liquid inlet is formed in a starting position of the one-way flow channel; a liquid outlet is formed in an ending position of the one-way flow channel. The unit is simple in structure and convenient to use; the culture process is easy to monitor; the large-scale animal cell wall adherence and suspension culture can be met.

The invention relates to a special method for testing the high throughput acute toxicity of an early life stage of fish, and belongs to the technical field of environment detection and assessment. According to the method, the sensibility of the fish at different life stages on toxic pollutants as well as the influence of conditions such as different quantities of test creatures, different exposed containers and the like on test results are discussed through developing a recombinant dilute solution for toxicity test, thus realizing high throughput on a larval fish acute toxicity test. The method is simple, convenient, economical, rapid and sensitive, has low requirements on experiment operation technical level, and is convenient for popularization and application.

The invention belongs to the field of biological medicine, and particularly relates to application of menstrual blood stem cells in preparation of drugs for treating intrauterine adhesion. A preparation method of the menstrual blood stem cells comprises the following steps: diluting an extracted menstrual blood sample by using PBS, slowly adding to the upper layer of a nuclear red blood cells membrane, centrifuging and separating a mononuclear cell layer, removing supernate, resuspending a complete culture solution for cell precipitation, inoculating in a cell culture bottle, and culturing in a culture box with 5% of CO2 at the temperature of 37 DEG C; when cells grow all over to 80-90%, carrying out digestion passage with 0.25% of trypsin, and after cytoplasm retracts and gaps among cells are increased, adding the complete culture solution to terminate digestion; repeatedly blowing and beating a bottle wall to form cell suspension; sucking the cell suspension into a centrifuge tube, centrifuging and removing supernate; adjusting cell concentration by the complete culture solution for cell precipitation, and inoculating in a culture bottle; and culturing in a culture box with 5% of CO2 at the temperature of 37 DEG C, wherein culture passage numbers are 2-3. The cells are high in survival rate, stable in character, easy to obtain and easy to amplify, and has remarkable treatment effect on intrauterine adhesion.

The invention discloses a medical bacterial culture device convenient in regulating a culture environment. The medical bacterial culture device comprises an incubator, a humidity control structure, anelectric control structure and moving structures; the bottom end of the incubator is provided with universal wheels; a culture bin is arranged in the incubator; the humidity control structure is installed at the rear end of the incubator; the electric control structure is installed at the left side end of the incubator; and the moving structures are arranged on the surfaces of a common culture box, a low-temperature culture box and a high-temperature culture box respectively. For the medical bacterial culture device provided by the invention, the common culture box, the low-temperature culture box and the high-temperature culture box which are arranged in the culture bin can be used for culturing bacteria living at different temperatures, so that the bacteria with different temperature requirements can be ensured to be stably cultured, the influences of temperature changes on other bacterial cultures can be reduced, and the bacteria with different temperature requirements can be simultaneously cultured; and a temperature and a humidity in the culture bin of the device can be measured through arranged temperature and humidity sensors.

The invention discloses a special culture medium for converting human pluripotent stem cells into extended pluripotent stem cells, and application of the special culture medium. According to the special culture medium, knockout DMEM/F12 and Neurobaal are mixed in a ratio of 1:1 as a basic culture medium, and the special culture medium further comprises the following components: B27, N2, a serum substitute, glutamate, non-essential amino acid, a double antibody, beta-mercaptoethanol, LCDM, a WNT pathway inhibitor and a ROCK inhibitor. According to the invention, the culture medium is combined with a matrix colloid system to culture human pluripotent stem cells, so that the human pluripotent stem cells can be successfully converted into adult extended pluripotent stem cells; and the culturemedium has definite chemical components, can reverse the hit of human pluripotent stem cells, converts the human pluripotent stem cells into extended pluripotent stem cells with bidirectional chimericability, is simple to operate, and has good application prospects.

The invention relates to the technical field of cell culture devices, and discloses a cell low-oxygen high-pressure culture device which comprises a culture box, a water tray is fixedly mounted on the inner bottom wall of the culture box, a supporting plate is fixedly mounted on the inner side wall of the culture box, and a cell culture dish is arranged at the top of the supporting plate; a fan and a sensor group are fixedly mounted on the inner top wall of the incubator, and an oxygen pipe, a nitrogen pipe and a carbon dioxide pipe are fixedly mounted on the outer wall of the incubator. By regulating and controlling parameters such as oxygen, pressure, temperature, carbon dioxide concentration and the like, combining with a culture medium meeting physiological needs, providing personalized culture conditions and simulating a microenvironment of the cells in vivo, the cells are stably cultured, and the characteristics of the cells in vivo are kept, so the cells and organoids show better in-vivo correlation in subsequent researches. The device is novel in design, and has the advantages that the microenvironment for cell growth can be accurately regulated and controlled, and the physiological environments of different parts in the body can be comprehensively simulated.

The invention discloses a method for cultivating rotifers. The method disclosed by the invention comprises the steps: arranging a cultivating tank according to the rotifer growing environment; evenlymixing yeast, fish meal, starch, yeast cream, saccharose, magnesium sulfate, monopotassium phosphate and water according to a certain proportion; fermenting about 12h under a certain temperature; after basic bait is fermented, filling a bolting silk mesh bag with the basic bait and hanging in cultivating tank water; adding photosynthetic bacteria into the tank; cultivating for 5 to 8 days from aninoculation day; harvesting when the rotifer density reaches 500 per milliliter or more; in the period, timely adding the bait according to a rotifer breeding speed and a residual bait amount in the mesh bag. The hanging bag cultivation mode avoids the phenomenon that the bait feeding amount is too much or too little; the raw materials are prepared into soft lumps by a method that the yeast and the fish meal are fermented, so that rotifer nutrition enrichment caused by single yeast nutrition is avoided; nutritional ingredients of the basic bait fermented by the yeast can be converted into nutrient substances of micromolecular protein peptide and the like; thus, the basic bait is easy for rotifers to absorb, bait nutrition is comprehensive, a utilization rate is high, and the cultivated rotifers have the advantages of strong physique, high survival rate and stable yield.

The invention discloses a bacterial culture and storage device applied to a high-humidity environment, and belongs to the technical field of bacterial culture.According to the scheme, in the bacterial culture process, a humidity monitoring mechanism monitors the humidity change in a sterile culture box through a humidity sensor, and if the humidity is too high, the sterile culture box is started; the air-drying moisturizing mechanism pulls a moving plate and a sealing plug to be far away from an air outlet through an electric telescopic rod, so that hot air accumulated in a heat conduction ball is released, discharged upwards under pulling of the moving plate and flows through the inner side and the outer side of the sterile incubator, the humidity in the sterile incubator is reduced, and humidity balance is kept; the liquid cooling humidity supply mechanism sprays potassium nitrate powder through an electric spray head, the potassium nitrate powder is dissolved in an aqueous solution, the temperature is reduced, water vapor in the monitoring cabin is liquefied when cooled, air is promoted to keep moist, under pushing of an extrusion push block, the moist air is extruded oppositely to flow, the dryness of the sterile incubator is reduced, and a constant-humidity environment is created. Growth and reproduction of bacteria are promoted, and the culture effect is enhanced.

The invention belongs to the field of stem cells, and discloses a primary isolation method for hair follicle stem cells. According to the method, scalp tissues are pretreated and neutral protease is added to digest the scalp tissues; after cleaning, complete hair follicles are pulled out in the hair follicle growth direction and inoculated into a culture bottle coated by L-polylysine; culture solution for stem cells are added to carry out culture; passage is carried out after the cell convergence degree is up to 80-90%. Experiments prove that the primary isolation method is capable of isolating a plenty of hair follicle stem cells, is good in cell activity and is capable of carrying out stable subsequent culture.

The invention relates to an Eriocheir hepatopancreas cell separation and primary culture method. The method comprises the following steps: (1) cleaning Eriocheir pancreatic tissues with cell separation liquid, cutting the cleaned Eriocheir pancreatic tissues to tissue blocks of 1mm<2> with a shear, adding a proper quantity of tissue blocks into the separation liquid, blowing and filtering with a suction tube, and centrifuging the filtrate to obtain yellow precipitate; (2) adding the yellow precipitate into a culture medium containing 100UI/ml penicillin and 100mug/ml streptomycin, blowing uniformly, inoculating into a culture plate, and culturing in a CO2 cell incubator. By adopting the method, Eriocheir hepatopancreas cells can be stably cultured till the survival rate with 72 hours is higher than 50 percent, a theoretical basis is laid for building of a subsequent cell line, and a cell tool is provided for researches in relevant fields of cell physiology, gene engineering and the like.

The invention relates to a tissue culture rapid propagation method for mini core-fragrant sweet potatoes. The tissue culture rapid propagation method specifically includes: picking the young and tender stem, with buds, of the mini core-fragrant sweet potatoes, shearing the stem with buds, shearing the top buds, and cleaning and sterilizing to obtain an explant for standby; sterilizing the explant, and performing primary culture on the explant to obtain a sterile culture system; cutting the stem, with one leaf and one bud, of the mini core-fragrant sweet potato after the primary culture, inoculating the stem to a subculture medium for multiplication culture, wherein the culture temperature of the primary culture and subculture is 25-28 DEG C, and culture time is 30 days; the light intensity of the first 15 days is 1600-1800lx, and the illumination time is 12 hours every day; the light intensity of the later 15 days is 3100-3800lx, and the illumination time is 15 hours every day. The tissue culture rapid propagation method has the advantages that the sterile culture system is obtained by using the primary culture, fast multiplication of the cut stem is achieved through the subculture, multiplication times can reach 13 times, and a stable and efficient tissue culture rapid propagation technological system for the mini core-fragrant sweet potatoes is built.

The invention relates to the technical field of bioengineering, and discloses an anti-pollution bioengineering cell culture dish cover which comprises a cover body, a transmission wheel is fixedly connected to the interior of the cover body, the outer side of the transmission wheel is sleeved with a rotating wheel, a rotating shaft is inserted into the outer side of the rotating wheel, a bevel gear is fixedly connected to the side, close to the transmission wheel, of the rotating shaft; a top block is fixedly connected to the outer side of the transmission wheel; and a travel switch is slidably connected to the inner wall of the rotating wheel. According to the anti-pollution bioengineering cell culture dish cover, a connector descends to drive a gear to rotate; the gear rotates to drive a worm gear to rotate; the worm gear rotates to drive a worm to rotate; the worm drives a lantern ring to rotate; the lantern ring drives a driving wheel to rotate through spike teeth and spike grooves; the driving wheel rotates to drive a pull rod to rotate; and the pull rod drives a valve to swing; and the valve and the suction pipe are matched for use, so that the effects of convenient sampling and stable culture are achieved.