33results about How to "Cultivate stable" patented technology

A yarn for a cell culture support is provided. A yarn for a cell culture support according to an embodiment of the present invention comprises a slit yarn in which a compressed nanofiber web is cut toa predetermined width. Accordingly, a microenvironment suitable for attachment, migration, proliferation, and differentiation of cultured cells is realized, thereby improving the survival rate of cells and proliferating the cells three-dimensionally. In addition, a support according to the present invention can stably proliferate the cells because the support exhibits sufficient mechanical strength to prevent a collapse, etc. thereof during the cell culture process. Further, the support according to the present invention can realize various sizes of pores by using the slit yarn formed from acompressed nanofiber web as yarn, such that an environment similar to an extracellular matrix is realized, thereby improving cell proliferation and the survival rate thereof.

The invention provides a micro-fluidic chip for in-situ contrast culture of animal tissues. The micro-fluidic chip is provided with a micro-fluidic network structure and a plurality of fluid inlets, wherein the micro-fluidic network structure adopts an array type and is used for culturing the animal tissues; the flow inlets are connected with a tissue culture array; by utilizing the laminar state of fluid from the inlets in a micro-fluidic passage and the gathering function of a tissue suspension from the middle inlet, the tissues are distributed to each row of culture units by multiple levels of Y-shaped flow separating areas, and each culture unit is used for fixing, culturing and observing the tissues; the different types of culture liquids are inputted by different inlets, the flow rate is adjusted, the tissues fixed in different rows are cultured in different types of culture liquids, and then the in-situ contrast culture of tissues on the same micro-fluidic chip is realized. The micro-fluidic chip has the advantage that by utilizing transparent material, the real-time monitoring under a microscope and the in-situ analysis of dynamic growth and differentiation process of tissues can be performed, so as to study the dynamics parameters of structure and function of animal tissues under the condition of stimulation by different culture environments.

The invention belongs to the field of stem cells, and discloses a primary separation method of skin mesenchymal stem cells. The skin tissue is pretreated, digested with Dispase II, washed and blown repeatedly to separate the cells from the tissue block, filtered and centrifuged. The obtained cell pellet was resuspended with Lonza complete medium and inoculated in a culture dish for 60-90 minutes; the non-adhered cell suspension was inoculated into a fibronectin-coated culture plate and cultured until the cell confluency reached 80%~ Passage after 90%. The cell membrane of skin mesenchymal stem cells isolated and cultured by the primary separation method of the present invention will not be damaged, the cell culture is stable, and mycoplasma pollution will not be introduced, a large number of skin mesenchymal stem cells can be separated, and the cell viability is good, which can Subsequent stable cultivation.

The invention provides a medical electric heating incubator, including a temperature sensor; the culture tank is a circular groove, arrayed on the top surface of the pumping plate, and nine culture tanks are arranged on each pumping plate; the height of the culture dish is The culture tank is twice as deep as the culture tank, and placed in the culture tank; the bottom of the left and right ends of the upper cover is provided with a rectangular strip, which is fixed on the top surface of the pumping plate through the cooperation of the strip and the rectangular grooves at the left and right ends of the top surface of the pumping plate; The temperature sensor is fixed on the top side of the inside of the box and is electrically connected to the microcomputer controller; the invention is easy to operate, has high internal temperature stability, and can maintain the temperature within a certain range when opening and closing the box door or when the power is accidentally cut off. Stability, to prevent large changes in temperature from affecting the collection data of experimental samples. At the same time, the petri dish in the box is placed in the culture tank, far away from the heating source, so that the heat generated will not be concentrated, and the experimental temperature at each position in the petri dish remains uniform, improving Experimental Accuracy.

The invention relates to an Eriocheir hepatopancreas cell separation and primary culture method. The method comprises the following steps: (1) cleaning Eriocheir pancreatic tissues with cell separation liquid, cutting the cleaned Eriocheir pancreatic tissues to tissue blocks of 1mm<2> with a shear, adding a proper quantity of tissue blocks into the separation liquid, blowing and filtering with a suction tube, and centrifuging the filtrate to obtain yellow precipitate; (2) adding the yellow precipitate into a culture medium containing 100UI / ml penicillin and 100mug / ml streptomycin, blowing uniformly, inoculating into a culture plate, and culturing in a CO2 cell incubator. By adopting the method, Eriocheir hepatopancreas cells can be stably cultured till the survival rate with 72 hours is higher than 50 percent, a theoretical basis is laid for building of a subsequent cell line, and a cell tool is provided for researches in relevant fields of cell physiology, gene engineering and the like.

The invention provides a copepoda indoor circulating culture tank. The copepoda indoor circulating culture tank comprises a culture tank and an air inflation pump, wherein a constant-temperature bar, a salinity meter and the air inflation pump are arranged in the culture tank; the constant-temperature bar is connected with the top part of the culture tank through a connecting plate, and is arranged in the culture tank; the salinity meter is connected with the top part of the culture tank through a connecting plate, and is arranged in the culture tank; the air inflation pump is arranged at the side surface of the culture tank; a slope platform is arranged at the bottom part of the culture tank; a filter net is arranged above the slope platform; a feeding device is arranged at the top part of the culture tank; a lamp tube is arranged at the back surface of the culture tank; a water drainage and collection pipe is arranged at the bottom part of the side surface of the culture tank; the culture tank is a light transmitting rubber glass culture tank, the top part of the culture tank is connected with an anti-escape plate, and the hole diameter of the filter net is 100 to 155mu m; an included angle between the slope platform and the bottom surface of the culture tank is 10 to 45 degrees. The copepoda indoor circulating culture tank has the advantage that by precisely regulating culture conditions of copepoda, adults, larvae and eggs are separated, so that the copepoda can be stably, efficiently and healthily cultured.

The invention discloses a wall adherence / suspension type cell culture unit as well as a device, a system and a method thereof and relates to the technical field of cell culture devices. The culture unit comprises a shell, wherein a plurality of cell growth plates are arranged inside the shell; the adjacent cell growth plates are alternately connected to the left side wall or the right side wall of the shell; the front side surfaces of the cell growth plates are fixed to a front side plate of the shell, and the rear side surfaces of the cell growth plates are fixed to a rear side plate of the shell; a gap is retained between the adjacent cell growth plates; a roundabout one-way flow channel is formed among the cell growth plates and the inner wall of the shell; a liquid inlet is formed in a starting position of the one-way flow channel; a liquid outlet is formed in an ending position of the one-way flow channel. The unit is simple in structure and convenient to use; the culture process is easy to monitor; the large-scale animal cell wall adherence and suspension culture can be met.

The invention belongs to the field of biotechnology, and in particular relates to the application of a compound containing a single pentasaccharide structural unit and a salt thereof in cell culture. The compound has a structure as shown in formula I, wherein the substituents have the meanings given in the specification, which can better control serum-free medium and compound-defined medium, and make the composition more definite, better in quality control, and better in quality. Low infection, more stable culture. The present invention also provides a medium comprising the compound shown in formula I, the medium has the advantages of clear chemical composition, small molecular weight, low cost, convenient quality control, efficient promotion of cell proliferation and maintenance of stem cell pluripotency.

The invention belongs to the field of stem cells, and discloses a primary separation method of hair follicle stem cells. The scalp tissue is pretreated and then digested with neutral protease. After cleaning, the complete hair follicles are pulled out along the hair follicle growth direction and inoculated with L-polylysine. Add special culture medium for stem cells to the coated culture flask, and culture when the cell confluency reaches 80‑90%. Experiments show that the primary separation method of the present invention can separate a large number of hair follicle stem cells, and the cells have good vigor and can be stably cultured subsequently.

The invention discloses a special culture medium for transforming human pluripotent stem cells into expanded pluripotent stem cells and its application. The medium is based on a 1:1 mixture of knockout DMEM / F12 and Neurobasal, and also includes the following components: B27, N2, serum replacement, glutamate, non-essential amino acids, double antibodies, β-mercaptoethanol , LCDM, WNT pathway inhibitors and ROCK inhibitors. Human pluripotent stem cells can be successfully transformed into human expanded pluripotent stem cells by using the culture medium combined with Matrigel system. The chemical composition of the culture medium is clear, it can reverse the fate of human pluripotent stem cells, and transform human pluripotent stem cells into expanded pluripotent stem cells with bidirectional chimerism, the operation is simple, and it has good application prospects.