33results about How to "Cultivate stable" patented technology

The invention discloses a wall adherence / suspension type cell culture unit as well as a device, a system and a method thereof and relates to the technical field of cell culture devices. The culture unit comprises a shell, wherein a plurality of cell growth plates are arranged inside the shell; the adjacent cell growth plates are alternately connected to the left side wall or the right side wall of the shell; the front side surfaces of the cell growth plates are fixed to a front side plate of the shell, and the rear side surfaces of the cell growth plates are fixed to a rear side plate of the shell; a gap is retained between the adjacent cell growth plates; a roundabout one-way flow channel is formed among the cell growth plates and the inner wall of the shell; a liquid inlet is formed in a starting position of the one-way flow channel; a liquid outlet is formed in an ending position of the one-way flow channel. The unit is simple in structure and convenient to use; the culture process is easy to monitor; the large-scale animal cell wall adherence and suspension culture can be met.

The invention relates to a special method for testing the high throughput acute toxicity of an early life stage of fish, and belongs to the technical field of environment detection and assessment. According to the method, the sensibility of the fish at different life stages on toxic pollutants as well as the influence of conditions such as different quantities of test creatures, different exposed containers and the like on test results are discussed through developing a recombinant dilute solution for toxicity test, thus realizing high throughput on a larval fish acute toxicity test. The method is simple, convenient, economical, rapid and sensitive, has low requirements on experiment operation technical level, and is convenient for popularization and application.

The invention relates to a culturing method for a dominant nitrosation bacterial community. The culturing method comprises the following three culturing stages: stage 1, enriching a mixed bacterial community of nitrosomas and nitromonas, wherein a microbial growth promoter A is used in the process of enrichment; stage 2, carrying out nitromonas elutriation by using a microbial growth promoter D and a high-temperature culture and normal-temperature culture alternate method so as to gradually improve the dominance of nitrosomas and beginning third-stage culture when a nitrosation rate is greater than 50%; and stage 3, reducing the content of dissolved oxygen or increasing a pH value, carrying out further nitromonas elutriation and stability domestication of nitrosomas by using the microbial growth promoter D and terminating culture when the nitrosation rate is stabilized and is 65% or more so as to obtain the dominant nitrosation bacterial community. The culturing method provided by the invention overcomes the problems of instability of partial nitrification in practical application, can shorten the starting time of nitrifying process, rapidly changes the process of a nitrifying reaction, broadens the application scope of partial nitrification and provides guarantee for actual application of a partial nitrification process to real projects.

The invention belongs to the field of biological medicine, and particularly relates to application of menstrual blood stem cells in preparation of drugs for treating intrauterine adhesion. A preparation method of the menstrual blood stem cells comprises the following steps: diluting an extracted menstrual blood sample by using PBS, slowly adding to the upper layer of a nuclear red blood cells membrane, centrifuging and separating a mononuclear cell layer, removing supernate, resuspending a complete culture solution for cell precipitation, inoculating in a cell culture bottle, and culturing in a culture box with 5% of CO2 at the temperature of 37 DEG C; when cells grow all over to 80-90%, carrying out digestion passage with 0.25% of trypsin, and after cytoplasm retracts and gaps among cells are increased, adding the complete culture solution to terminate digestion; repeatedly blowing and beating a bottle wall to form cell suspension; sucking the cell suspension into a centrifuge tube, centrifuging and removing supernate; adjusting cell concentration by the complete culture solution for cell precipitation, and inoculating in a culture bottle; and culturing in a culture box with 5% of CO2 at the temperature of 37 DEG C, wherein culture passage numbers are 2-3. The cells are high in survival rate, stable in character, easy to obtain and easy to amplify, and has remarkable treatment effect on intrauterine adhesion.

The invention provides a culture medium, a culture method and a detection method of a thyroid cancer organoid, the culture medium comprises a basic culture medium, B-27, N-acetylcysteine, nicotinamide, R-spondin 1 recombinant protein and other components, the culture medium has high thyroid cancer organoid culture success rate, high passage frequency, and good passage stability after repeated cryopreservation and resuscitation; the thyroid cancer organoid obtained by adopting the culture medium and the culture method is high in reducibility; and the detection method is comprehensive and accurate.

The invention discloses a medical bacterial culture device convenient in regulating a culture environment. The medical bacterial culture device comprises an incubator, a humidity control structure, anelectric control structure and moving structures; the bottom end of the incubator is provided with universal wheels; a culture bin is arranged in the incubator; the humidity control structure is installed at the rear end of the incubator; the electric control structure is installed at the left side end of the incubator; and the moving structures are arranged on the surfaces of a common culture box, a low-temperature culture box and a high-temperature culture box respectively. For the medical bacterial culture device provided by the invention, the common culture box, the low-temperature culture box and the high-temperature culture box which are arranged in the culture bin can be used for culturing bacteria living at different temperatures, so that the bacteria with different temperature requirements can be ensured to be stably cultured, the influences of temperature changes on other bacterial cultures can be reduced, and the bacteria with different temperature requirements can be simultaneously cultured; and a temperature and a humidity in the culture bin of the device can be measured through arranged temperature and humidity sensors.

The invention discloses a special culture medium for converting human pluripotent stem cells into extended pluripotent stem cells, and application of the special culture medium. According to the special culture medium, knockout DMEM / F12 and Neurobaal are mixed in a ratio of 1:1 as a basic culture medium, and the special culture medium further comprises the following components: B27, N2, a serum substitute, glutamate, non-essential amino acid, a double antibody, beta-mercaptoethanol, LCDM, a WNT pathway inhibitor and a ROCK inhibitor. According to the invention, the culture medium is combined with a matrix colloid system to culture human pluripotent stem cells, so that the human pluripotent stem cells can be successfully converted into adult extended pluripotent stem cells; and the culturemedium has definite chemical components, can reverse the hit of human pluripotent stem cells, converts the human pluripotent stem cells into extended pluripotent stem cells with bidirectional chimericability, is simple to operate, and has good application prospects.

The invention relates to the technical field of cell culture devices, and discloses a cell low-oxygen high-pressure culture device which comprises a culture box, a water tray is fixedly mounted on the inner bottom wall of the culture box, a supporting plate is fixedly mounted on the inner side wall of the culture box, and a cell culture dish is arranged at the top of the supporting plate; a fan and a sensor group are fixedly mounted on the inner top wall of the incubator, and an oxygen pipe, a nitrogen pipe and a carbon dioxide pipe are fixedly mounted on the outer wall of the incubator. By regulating and controlling parameters such as oxygen, pressure, temperature, carbon dioxide concentration and the like, combining with a culture medium meeting physiological needs, providing personalized culture conditions and simulating a microenvironment of the cells in vivo, the cells are stably cultured, and the characteristics of the cells in vivo are kept, so the cells and organoids show better in-vivo correlation in subsequent researches. The device is novel in design, and has the advantages that the microenvironment for cell growth can be accurately regulated and controlled, and the physiological environments of different parts in the body can be comprehensively simulated.

The invention discloses a method for cultivating rotifers. The method disclosed by the invention comprises the steps: arranging a cultivating tank according to the rotifer growing environment; evenlymixing yeast, fish meal, starch, yeast cream, saccharose, magnesium sulfate, monopotassium phosphate and water according to a certain proportion; fermenting about 12h under a certain temperature; after basic bait is fermented, filling a bolting silk mesh bag with the basic bait and hanging in cultivating tank water; adding photosynthetic bacteria into the tank; cultivating for 5 to 8 days from aninoculation day; harvesting when the rotifer density reaches 500 per milliliter or more; in the period, timely adding the bait according to a rotifer breeding speed and a residual bait amount in the mesh bag. The hanging bag cultivation mode avoids the phenomenon that the bait feeding amount is too much or too little; the raw materials are prepared into soft lumps by a method that the yeast and the fish meal are fermented, so that rotifer nutrition enrichment caused by single yeast nutrition is avoided; nutritional ingredients of the basic bait fermented by the yeast can be converted into nutrient substances of micromolecular protein peptide and the like; thus, the basic bait is easy for rotifers to absorb, bait nutrition is comprehensive, a utilization rate is high, and the cultivated rotifers have the advantages of strong physique, high survival rate and stable yield.

The invention discloses a strain culture device for edible fungus production. The device comprises an incubator, a side support frame and a tray, a side support frame is fixedly mounted on the inner side wall of the incubator, a top bracket is mounted at the top of the inner side of the incubator, a hose is installed at the top of the first sliding block in a penetrating mode, a sprinkler head isfixedly installed on the water pipe, a reset spring is installed at the front end of the first sliding block, a clamping and placing mechanism is installed on the tray, a cam is arranged under the clamping and placing mechanism, a rotating shaft is installed in the middle of the cam in a penetrating mode, and the rotating shaft penetrates through the tray. According to the strain culture device for edible fungus production, a strain culture medium needing to be cultured can be isolated from the outside, the strain culture process is more stable, the culture medium can be fixed and clamped, theculture medium is always in a stable state in the moving process of the tray at the bottom of the culture medium, the culture medium in the incubator can be comprehensively irrigated through the hoseand the water pipe, and the strain culture state is better.

A yarn for a cell culture support is provided. A yarn for a cell culture support according to an embodiment of the present invention comprises a slit yarn in which a compressed nanofiber web is cut toa predetermined width. Accordingly, a microenvironment suitable for attachment, migration, proliferation, and differentiation of cultured cells is realized, thereby improving the survival rate of cells and proliferating the cells three-dimensionally. In addition, a support according to the present invention can stably proliferate the cells because the support exhibits sufficient mechanical strength to prevent a collapse, etc. thereof during the cell culture process. Further, the support according to the present invention can realize various sizes of pores by using the slit yarn formed from acompressed nanofiber web as yarn, such that an environment similar to an extracellular matrix is realized, thereby improving cell proliferation and the survival rate thereof.

The invention relates to the technical field of hair follicle stem cell culture, and specifically relates to an injection device for hair follicle stem cell culture and a using method. The device comprises a shell body, a culture dish, a liquid-injection structure, an injection structure, an auxiliary structure, a protective structure and a fixed structure. The injection structure arranged on theshell body facilitates the injection of stem cells into the interior of the culture dish without contacting with air, and the culture dish is stably installed inside the shell body through the cooperation use of the protective structure, which is conducive to the protection of stem cell culture while injecting stem cells, and preventing stem cells from contacting with air. Through the linkage of the injection structure and the liquid-injection structure, after the stem cells are injected into the culture dish by the injection structure, nutrition solution is injected by the liquid-injection structure from the injection structure into the culture dish for the growth of stem cells, the interior of the injection structure is washed, which makes the interior of the injection structure clean while some attached stem cells are flushed into the interior of the culture dish, and at the same time, the nutrition solution enters the interior of the culture dish easily and smoothly from the injection structure.

The invention discloses an NK cell culture kit and a usage method thereof and an NK cell obtained therefrom. The kit comprises an NK kit set and an NK consumable kit set, wherein the consumable kit set consists of a mononuclear lymphocyte cell separation centrifugal tube, a portable cell sample adding pre-filling stand, a cell culture bag tray and a cell culture bag; and the reagent box set consists of a pretreatment factor NK-A, a pretreatment factor NK-B, an amplification factor NK-C, an amplification factor NK-D, an amplification factor NK-E and a serum-free lymphocytes cell culture medium. More than 2*10<9> lymphocyte cells can be amplified within short time by matching the NK culture kit with basic experimental consumables, wherein the ratio of the NK cells (CD3-CD56+) can achieve more than 60%, thus providing convenience for amplifying NK cells in vitro on a large scale and conducting related studies and clinical trials.

The invention discloses a bacterial culture and storage device applied to a high-humidity environment, and belongs to the technical field of bacterial culture.According to the scheme, in the bacterial culture process, a humidity monitoring mechanism monitors the humidity change in a sterile culture box through a humidity sensor, and if the humidity is too high, the sterile culture box is started; the air-drying moisturizing mechanism pulls a moving plate and a sealing plug to be far away from an air outlet through an electric telescopic rod, so that hot air accumulated in a heat conduction ball is released, discharged upwards under pulling of the moving plate and flows through the inner side and the outer side of the sterile incubator, the humidity in the sterile incubator is reduced, and humidity balance is kept; the liquid cooling humidity supply mechanism sprays potassium nitrate powder through an electric spray head, the potassium nitrate powder is dissolved in an aqueous solution, the temperature is reduced, water vapor in the monitoring cabin is liquefied when cooled, air is promoted to keep moist, under pushing of an extrusion push block, the moist air is extruded oppositely to flow, the dryness of the sterile incubator is reduced, and a constant-humidity environment is created. Growth and reproduction of bacteria are promoted, and the culture effect is enhanced.

The invention discloses a self-stabilization oxygenation type cell culture box. The self-stabilization oxygenation type cell culture box comprises a shell, wherein a box body is fixedly connected to the inner wall of the shell, an air bag is fixedly connected to the lower end of the box body, the lower end of the air bag is elastically connected with the inner bottom of the shell through springs,and a sliding plug cavity is formed in the lower end of the box body. Two attracted magnetic sliding plugs are symmetrically connected to the inner wall of the sliding plug cavity in a sealed and sliding mode, an air suction bag is jointly and fixedly connected to the side walls close to each other, of the two magnetic sliding plugs, and the inner wall of the air bag is fixedly connected with theinner wall of the air suction bag through a one-way exhaust pipe. Air in the air bag enters the air suction bag through vibration of the shell, and then the two magnetic sliding plugs are pushed to slide in the direction away from each other, that is, the two magnetic sliding plugs are driven to slide in the direction away from each other through the vertical impact force of the vibration of the shell on the box body. The work of the shell on the box body is converted into magnetic potential energy of the magnetic sliding plugs along the parallel direction of the lower end of the box body, sothat the cell activity is ensured.

The invention belongs to the field of stem cells, and discloses a primary isolation method for hair follicle stem cells. According to the method, scalp tissues are pretreated and neutral protease is added to digest the scalp tissues; after cleaning, complete hair follicles are pulled out in the hair follicle growth direction and inoculated into a culture bottle coated by L-polylysine; culture solution for stem cells are added to carry out culture; passage is carried out after the cell convergence degree is up to 80-90%. Experiments prove that the primary isolation method is capable of isolating a plenty of hair follicle stem cells, is good in cell activity and is capable of carrying out stable subsequent culture.

The invention relates to an Eriocheir hepatopancreas cell separation and primary culture method. The method comprises the following steps: (1) cleaning Eriocheir pancreatic tissues with cell separation liquid, cutting the cleaned Eriocheir pancreatic tissues to tissue blocks of 1mm<2> with a shear, adding a proper quantity of tissue blocks into the separation liquid, blowing and filtering with a suction tube, and centrifuging the filtrate to obtain yellow precipitate; (2) adding the yellow precipitate into a culture medium containing 100UI / ml penicillin and 100mug / ml streptomycin, blowing uniformly, inoculating into a culture plate, and culturing in a CO2 cell incubator. By adopting the method, Eriocheir hepatopancreas cells can be stably cultured till the survival rate with 72 hours is higher than 50 percent, a theoretical basis is laid for building of a subsequent cell line, and a cell tool is provided for researches in relevant fields of cell physiology, gene engineering and the like.

The invention provides a medical electric heating culture box, which comprises a temperature sensor, wherein culture grooves are round grooves and are arrayed on the top surfaces of pulling plates; nine culture grooves are totally arranged on each pulling plate; the height of culture vessels is twice of the groove depth of the culture grooves; the culture vessels are put in the culture grooves; rectangular strips are arranged at the bottoms of the left and right ends of an upper cover; the upper cover is fixed on the top surface of the pulling plates through the matching of the strips with therectangular grooves formed in the left and right ends of the top surfaces of the pulling plates; the temperature sensor is fixed on the top side surface inside a box body, and is electrically connected with a microcomputer controller. The medical electric heating culture box has the advantages that the operation is simple and convenient; the inside temperature stability is high; the temperature stability can be maintained in a certain range during box door opening or closing or unexpected power failure; the experiment sample data collection influence caused by great temperature change is prevented; meanwhile, the culture vessels in the box are put into the culture grooves and are far away from a heating source, so that the heat concentration cannot be generated; the experiment temperaturein each position of each culture vessel maintains uniform; the experiment accuracy is improved.

The invention provides a micro-fluidic chip for in-situ contrast culture of animal tissues. The micro-fluidic chip is provided with a micro-fluidic network structure and a plurality of fluid inlets, wherein the micro-fluidic network structure adopts an array type and is used for culturing the animal tissues; the flow inlets are connected with a tissue culture array; by utilizing the laminar state of fluid from the inlets in a micro-fluidic passage and the gathering function of a tissue suspension from the middle inlet, the tissues are distributed to each row of culture units by multiple levels of Y-shaped flow separating areas, and each culture unit is used for fixing, culturing and observing the tissues; the different types of culture liquids are inputted by different inlets, the flow rate is adjusted, the tissues fixed in different rows are cultured in different types of culture liquids, and then the in-situ contrast culture of tissues on the same micro-fluidic chip is realized. The micro-fluidic chip has the advantage that by utilizing transparent material, the real-time monitoring under a microscope and the in-situ analysis of dynamic growth and differentiation process of tissues can be performed, so as to study the dynamics parameters of structure and function of animal tissues under the condition of stimulation by different culture environments.

The invention belongs to the field of stem cells, and discloses a primary separation method of skin mesenchymal stem cells. The skin tissue is pretreated, digested with Dispase II, washed and blown repeatedly to separate the cells from the tissue block, filtered and centrifuged. The obtained cell pellet was resuspended with Lonza complete medium and inoculated in a culture dish for 60-90 minutes; the non-adhered cell suspension was inoculated into a fibronectin-coated culture plate and cultured until the cell confluency reached 80%~ Passage after 90%. The cell membrane of skin mesenchymal stem cells isolated and cultured by the primary separation method of the present invention will not be damaged, the cell culture is stable, and mycoplasma pollution will not be introduced, a large number of skin mesenchymal stem cells can be separated, and the cell viability is good, which can Subsequent stable cultivation.

The invention relates to an Eriocheir hepatopancreas cell separation and primary culture method. The method comprises the following steps: (1) cleaning Eriocheir pancreatic tissues with cell separation liquid, cutting the cleaned Eriocheir pancreatic tissues to tissue blocks of 1mm<2> with a shear, adding a proper quantity of tissue blocks into the separation liquid, blowing and filtering with a suction tube, and centrifuging the filtrate to obtain yellow precipitate; (2) adding the yellow precipitate into a culture medium containing 100UI / ml penicillin and 100mug / ml streptomycin, blowing uniformly, inoculating into a culture plate, and culturing in a CO2 cell incubator. By adopting the method, Eriocheir hepatopancreas cells can be stably cultured till the survival rate with 72 hours is higher than 50 percent, a theoretical basis is laid for building of a subsequent cell line, and a cell tool is provided for researches in relevant fields of cell physiology, gene engineering and the like.

The invention provides a copepoda indoor circulating culture tank. The copepoda indoor circulating culture tank comprises a culture tank and an air inflation pump, wherein a constant-temperature bar, a salinity meter and the air inflation pump are arranged in the culture tank; the constant-temperature bar is connected with the top part of the culture tank through a connecting plate, and is arranged in the culture tank; the salinity meter is connected with the top part of the culture tank through a connecting plate, and is arranged in the culture tank; the air inflation pump is arranged at the side surface of the culture tank; a slope platform is arranged at the bottom part of the culture tank; a filter net is arranged above the slope platform; a feeding device is arranged at the top part of the culture tank; a lamp tube is arranged at the back surface of the culture tank; a water drainage and collection pipe is arranged at the bottom part of the side surface of the culture tank; the culture tank is a light transmitting rubber glass culture tank, the top part of the culture tank is connected with an anti-escape plate, and the hole diameter of the filter net is 100 to 155mu m; an included angle between the slope platform and the bottom surface of the culture tank is 10 to 45 degrees. The copepoda indoor circulating culture tank has the advantage that by precisely regulating culture conditions of copepoda, adults, larvae and eggs are separated, so that the copepoda can be stably, efficiently and healthily cultured.

The invention relates to a tissue culture rapid propagation method for mini core-fragrant sweet potatoes. The tissue culture rapid propagation method specifically includes: picking the young and tender stem, with buds, of the mini core-fragrant sweet potatoes, shearing the stem with buds, shearing the top buds, and cleaning and sterilizing to obtain an explant for standby; sterilizing the explant, and performing primary culture on the explant to obtain a sterile culture system; cutting the stem, with one leaf and one bud, of the mini core-fragrant sweet potato after the primary culture, inoculating the stem to a subculture medium for multiplication culture, wherein the culture temperature of the primary culture and subculture is 25-28 DEG C, and culture time is 30 days; the light intensity of the first 15 days is 1600-1800lx, and the illumination time is 12 hours every day; the light intensity of the later 15 days is 3100-3800lx, and the illumination time is 15 hours every day. The tissue culture rapid propagation method has the advantages that the sterile culture system is obtained by using the primary culture, fast multiplication of the cut stem is achieved through the subculture, multiplication times can reach 13 times, and a stable and efficient tissue culture rapid propagation technological system for the mini core-fragrant sweet potatoes is built.

The invention provides a yarn for a cell culture support. The yarn for a cell culture scaffold according to an embodiment of the present invention includes a slit yarn obtained by cutting a compressed nanofiber web into a predetermined width. Thus, by realizing a microenvironment suitable for attachment, migration, proliferation, and differentiation of cultured cells, the survival rate of cells can be improved and cells can be cultured three-dimensionally. Also, the scaffold according to the present invention has sufficient mechanical strength to prevent the scaffold from collapsing or the like during the cultivation of cells, thereby allowing cells to proliferate stably. Further, the scaffold according to the present invention can realize air pores of various sizes by using slitting yarns formed of compressed nanofibrous nets as yarns, thereby improving cell proliferation and survival rate.

The invention relates to the technical field of bioengineering, and discloses an anti-pollution bioengineering cell culture dish cover which comprises a cover body, a transmission wheel is fixedly connected to the interior of the cover body, the outer side of the transmission wheel is sleeved with a rotating wheel, a rotating shaft is inserted into the outer side of the rotating wheel, a bevel gear is fixedly connected to the side, close to the transmission wheel, of the rotating shaft; a top block is fixedly connected to the outer side of the transmission wheel; and a travel switch is slidably connected to the inner wall of the rotating wheel. According to the anti-pollution bioengineering cell culture dish cover, a connector descends to drive a gear to rotate; the gear rotates to drive a worm gear to rotate; the worm gear rotates to drive a worm to rotate; the worm drives a lantern ring to rotate; the lantern ring drives a driving wheel to rotate through spike teeth and spike grooves; the driving wheel rotates to drive a pull rod to rotate; and the pull rod drives a valve to swing; and the valve and the suction pipe are matched for use, so that the effects of convenient sampling and stable culture are achieved.