Low-oxygen high-pressure cell culture device

Abstract

The invention relates to the technical field of cell culture devices, and discloses a cell low-oxygen high-pressure culture device which comprises a culture box, a water tray is fixedly mounted on the inner bottom wall of the culture box, a supporting plate is fixedly mounted on the inner side wall of the culture box, and a cell culture dish is arranged at the top of the supporting plate; a fan and a sensor group are fixedly mounted on the inner top wall of the incubator, and an oxygen pipe, a nitrogen pipe and a carbon dioxide pipe are fixedly mounted on the outer wall of the incubator. By regulating and controlling parameters such as oxygen, pressure, temperature, carbon dioxide concentration and the like, combining with a culture medium meeting physiological needs, providing personalized culture conditions and simulating a microenvironment of the cells in vivo, the cells are stably cultured, and the characteristics of the cells in vivo are kept, so the cells and organoids show better in-vivo correlation in subsequent researches. The device is novel in design, and has the advantages that the microenvironment for cell growth can be accurately regulated and controlled, and the physiological environments of different parts in the body can be comprehensively simulated.

Description

technical field [0001] The invention relates to the technical field of cell culture devices, in particular to a cell culture device with low oxygen and high pressure. Background technique [0002] Long-term studies have shown that cells in various tissue parts of the human body are generally in a microenvironment of low oxygen and high pressure. At the same time, under the regulation of different oxygen and pressure, cells will have different changes in metabolism, gene and protein levels, which will affect the performance of cells in shape and behavior. The regulation of microenvironment is an effective means to control cells. [0003] For example, when there is a wound on the surface of the skin, blood will flow out. The reason is that the pressure in the human body is greater than the atmospheric pressure. Because of this, we know that the cells in the human body are under pressure all the time, and when the cells leave the body, The original pressure then disappears, a...

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Problems solved by technology

[0004] However, the ordinary cell incubator cannot adjust the pressure on the cells, and cannot accurately simulate the microenvironment of the cells in the body, so it cannot make the organoid cells derived from the primary tumor grow normally and reflect its in vivo stability in subsequent experiments. Physiological activity, which is very unfavorable for cell culture

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