358 results about "Growth cell" patented technology

The invention provides a cell growth dynamics prediction method and device based on a space-time image sequence, and belongs to the technical field of computer vision and deep learning. The method comprises the following steps: acquiring a multi-focal-plane space-time image sequence containing a cell complete growth process; preprocessing and normalizing images in the image sequence to obtain a final test image sequence; and inputting the test image sequence into a cell growth dynamics prediction model consisting of a multi-scale feature extraction module and a time sequence modeling module which are connected in sequence to obtain a cell growth stage identification result corresponding to each time point image in the sequence so as to realize cell growth dynamics prediction. The method can effectively solve the problems of poor adaptability, insufficient stability, high labeling cost and the like when a traditional model is used for processing the dynamic medical image sequence, and has remarkable advantages and application prospects in the aspect of dynamic prediction of the cell growth space-time image sequence.

A device and method for assembling objects by generating an axial acoustic radiation force in order to position the objects on pressure nodes and / or antinodes and generating a transverse acoustic radiation force in order to move the thus positioned objects on a porous membrane contact structure.

Disclosed in the present invention are a gel having a regulatable free water content and morphology, a preparation method therefor, and the use thereof. The method comprises: S1, preparing a solution A from a natural polymer material; S2, mixing a small molecule material containing a positively charged polar group with the solution A to obtain a composite solution B; S3, mixing the composite solution B with a regulator to obtain a composite solution C, stirring same, then adding a condensing agent to the reaction system, and carrying out a curing crosslinking reaction; and S4, removing the regulator and the condensing agent to obtain the gel. By adjusting the concentrations of the natural polymer material and the regulator, gels having different morphologies can be prepared; the gel undergoes a physical process to remove hydrogen bond interaction between the gel and free water, so that the content of free water attached to the surface of the gel can be regulated, thereby regulating the physical and chemical properties of the gel. The gel of the present invention has regulatable morphology and controllable elastic modulus, and has many advantages such as good lubrication effect, good biocompatibility and biodegradability, capability of being biologically active, and capabilities of stimulating cell growth and accelerating wound repair.

The invention discloses an anisotropic electrostatic spinning patch with ultrasonic response and a preparation method thereof, a fiber membrane with a controllable anisotropic structure is constructed by a biodegradable polymer, antibacterial nanoparticles and a photoinitiator system through a step-by-step directional electrostatic spinning process, so that the mechanical matching property of the fiber membrane is improved; cell growth can be directionally guided and tissue regeneration can be accelerated by combining the piezoelectric material characteristics of the introduced biodegradable polymer with piezoelectric characteristics and electric field stimulation induced by the fiber topological structure; meanwhile, a zwitterionic coating is generated on the surface of the fiber membrane in situ by utilizing a single-sided photocuring technology, and an anti-adhesion function is realized on the single side of the fiber membrane. Furthermore, the soft tissue repair patch which has multiple functions of adjustable mechanical property, infection resistance, adhesion resistance and the like and has better structural mechanical suitability, function integration and tissue regeneration guiding capability is prepared by adopting the anisotropic electrostatic spinning patch with ultrasonic response.

Methods, compositions and uses for inhibiting the growth in blood cancer cells in a patient with one or more of a caffeic acid phenpropyl ester (GL8) analogue selected from the group consisting of As26, J229, J91, LL27, LL23, HM7, As25, MT26, and J205. The blood cancer cells can be myeloma, lymphoma and leukemia cells. The methods, compositions and uses can be in conjunction with the use of an IMiD to treat a patient. The compositions can include a pharmaceutically acceptable carrier, adjuvant or vehicle, a pharmaceutically acceptable salt or dietary supplement.

The invention discloses application of beta-nicotinamide mononucleotide in regulation and control of gene editing efficiency, belongs to the field of gene editing treatment, and finds that the beta-nicotinamide mononucleotide (NMN) can efficiently inhibit the activity of CRISPR-Cas9, CRISPR-Cas12 and CRISPR-Cas13 systems in a broad-spectrum manner for the first time. The application comprises emergency blocking of off-target effect in gene editing clinical treatment, biological safety prevention and control of a virus vector gene editing system, and CRISPR activity regulation and control of in-vitro non-diagnostic purpose. Experiments show that NMN can inhibit CRISPR-mediated gene damage and cell death in a cell model, the inhibition efficiency in an in-vitro enzyme digestion system reaches 68.7%, and cell growth or transfection efficiency is not affected. The invention provides an innovative solution for safe application of CRISPR (clustered regularly interspaced short palindromic repeats) technology, and the NMN is approved to be taken orally as a health care product, so that the NMN has extremely strong clinical application potential. Compared with the existing CRISPR (clustered regularly interspaced short palindromic repeats)-resistant protein or synthetic small-molecule inhibitor, the NMN has the advantages of endogenous property, high biocompatibility, good oral safety and the like.

The invention discloses a preparation method of a novel composite carbon source. The preparation method comprises the following steps: S1, preparing a solution; s2, preparing a culture medium; s3, extracting a genome; s4, extraction of plasmid DNA (deoxyribonucleic acid); s5, preparation of agarose gel electrophoresis; s6, constructing a linearized vector plasmid; s7, preparing an electrotransfection competent cell; and S8, electric conversion treatment. According to the preparation method of the novel composite carbon source, pfkA and pfkB can be respectively, independently and simultaneously knocked out when the carbon source is prepared by utilizing a gene editing technology, the consumption of a glucosamine precursor 6-fructose phosphate in an EMP pathway can be reduced, more glucose is used for synthesizing a target product, and by optimizing the preparation process of the composite carbon source, the yield of the target product is improved. The shake-flask fermentation yield of the glucosamine can be obviously improved, the conversion rate of the composite carbon source is also improved, and cell growth and synthesis of the target product glucosamine can be reasonably balanced, so that the yield of the glucosamine is improved.

The application discloses a kind of live cell dynamic monitoring method, comprising the following steps: obtaining the cell scanning picture group that live cell grown in culture vessel is photographed under specified condition, and the picture is preprocessed;Cell scanning picture group after pre-processing is input into trained cell identification model, and output cell growth state chart group;Cell growth state chart group is input into trained survival rate calculation model, and the survival rate of cell in each cell growth state chart is output;The cell survival rate numerical value obtained is drawn into cell survival rate-time curve chart;The cell survival rate-time curve chart generated is combined with nonlinear regression statistical method, and the half inhibitory concentration value of the efficacy of specified parameter calculation is selected.The growth state of cell under specified condition is monitored, and the survival rate of cell and the half inhibitory concentration value of the efficacy are calculated based on these data;It can significantly improve the efficiency and accuracy in the field of drug research and development, drug screening, treatment scheme effect, etc.

To provide a cell proliferation system, proliferation method, control method, and culture medium bag capable of proliferating various adherent cells and suspension cells. [Solution] Cells are grown in a bioreactor and activated by an activator (e.g., a soluble activator complex). The nutrient supply and gas exchange functions of an automated closed cell proliferation system make it possible to seed at, for example, a low seeding density. By manipulating the parameters of the cell growth environment, cells are placed in specific locations within the bioreactor for efficient exchange of nutrients and gases. System parameters are adjusted to shear any cell colonies that may form during the proliferation phase. Metabolic concentrations are controlled to improve cell growth and viability. Cell retention within the bioreactor is controlled. In some embodiments, the cells include T cells. In other embodiments, the cells include a T cell subpopulation, for example, regulatory T cells (Tregs).

This invention relates to the fields of bioengineering and fermentation engineering, and discloses a genetically engineered *Streptomyces albopictus* strain that produces high levels of ε-polylysine and its fermentation production process. This genetically engineered strain uses *Streptomyces albopictus* strains acclimated to ε-polylysine tolerance as a chassis, and its genome integrates... ppc and dapf Through expression box, heterogeneous asd Expression cassettes, containing secretory signal peptides lysp Fusion expression cassettes and D404A / K499A point mutations pls Expression cassette. The fermentation process employs a two-stage pH control strategy, maintaining a neutral environment during the cell growth phase and adjusting to an acidic environment during product synthesis. This invention improves ε-polylysine yield through a synergistic strategy of enhancing precursor supply, constructing efflux detoxification channels, and increasing synthase activity; combined with an acidic fermentation process, it effectively inhibits product degradation, yielding a high-molecular-weight and highly uniform target product suitable for industrial production.

This invention relates to the fields of microbial genetic engineering and fermentation biomanufacturing technology, specifically disclosing a recombinant Corynebacterium glutamicum with high 5-aminolevulinic acid (ALA) production and its applications. Addressing the problems of existing strains such as crude enzyme activity regulation, inhibited cell growth, the need for external inhibitors, and low yield, this invention uses Corynebacterium glutamicum as a host and removes the gdhA gene without scarring to reduce precursor competitive metabolism. Site-directed mutations of the endogenous hemB gene (S288A, D128E, R231K) are performed to moderately reduce dehydratase activity. A high-expression plasmid pXMJ19-hemA containing the hemA gene from Rhodopsinus capsulatum is constructed and introduced into the modified strain to obtain a recombinant strain with optimized triple metabolism. This strain reduces product degradation without the need for external dehydratase inhibitors, does not affect cell growth, and achieves a yield up to 4.30 times higher than the control after 42 hours of fermentation, with ALA accumulation exceeding 8.9 g / L. The strain exhibits stable characteristics, the process is simple, and it has good industrial application value.

Systems and methods for encapsulating cells in hydrogel tubes for cell growth within hydrogel tubes are described. In aspects, a system includes a plurality of peristaltic pumps and a plurality of pulse dampeners to provide oscillatory-controlled flow rates of each of a cell solution, a hydrogel solution, and a cross-linking solution to an extruder configured to form hollow hydrogel tubes having suspended biological cells in an interior

The subject matter of this specification can be implemented in, among other things, methods, systems, computer-readable storage medium. A method can include receiving cell growth data with a current cell culture of a cell growth system. The cell growth data includes growth input parameter values indicative of a growth rate of the current cell culture. The method can further include identifying, using a first machine learning model, a prescriptive action to alter a yield of a target product of the current cell culture based on the cell growth data. The prescriptive action modifies a metabolism rate of the current cell culture. The method further includes performing at least one of displaying the identified prescriptive on a graphical user interface (GUI) and / or causing the cell growth system to perform the identified prescriptive action.

The invention provides a cell reaction bag with a double-gas-path ventilation device, the cell reaction bag comprises a reaction bag body, a ventilation device and a stirring device, the ventilation device comprises a ventilation disc, the ventilation disc is provided with a first hole group and a second hole group, and the ventilation disc is internally provided with a first channel and a second channel which are respectively communicated with the first hole group and the second hole group; the bottom of the ventilation disc is provided with a first air inlet and a second air inlet which are communicated with the first channel and the second channel respectively. According to the ventilation device disclosed by the invention, two groups of holes with different sizes and corresponding channels are arranged, so that double-gas-path ventilation is formed, different gases can be respectively introduced, large bubbles are generated on the outer side of the ventilation disc, and micro bubbles are generated on the inner side of the ventilation disc; the gas dissolving efficiency is improved, uniform gas distribution is ensured, and uniform growth of cells is facilitated; different requirements on gases such as oxygen and carbon dioxide in the cell culture process can be accurately controlled, and a more suitable gas environment is provided for cell growth.

The utility model discloses an angle-adjustable rotary tank type mesenchymal stem cell balling device, which relates to the technical field of mesenchymal stem cell balling, is arranged in a temperature control incubator for culturing mesenchymal stem cell balling, and comprises a bottle body for containing mesenchymal stem cells and a container for containing ultrasonic buffer water, the container is fixedly arranged on a horizontal bearing surface in the temperature control incubator, the bottle body is obliquely arranged, the bottom of the bottle body is positioned in the ultrasonic buffer water liquid of the container, the bottle body is driven to move by a driving device fixed on the horizontal bearing surface, and the driving device is configured to enable the bottle body to vibrate back and forth in the container along the horizontal direction and simultaneously drive the bottle body to move back and forth. And the bottle body also rotates around the axial direction of the bottle body. According to the utility model, the culture bottle simultaneously performs compound motion of horizontal oscillation and self rotation, so that the cell growth can be more uniformly stimulated, and the problem of poor balling effect caused by single rotation in the traditional method is solved. Through the movement mode, the cells are distributed more uniformly in the culture solution, and the formed spheres are more consistent in size and higher in quality.

The invention provides a chip system which comprises a cell growth area, the cell growth area comprises a cell layer and a plurality of first scaffolds, the cell layer is used for generating viruses, and each first scaffold comprises a plurality of hydrogel layers; the multiple hydrogel layers are stacked to form a groove, and pores of the multiple hydrogel layers are sequentially reduced in the direction from the interior to the exterior of the groove; the groove is used for accommodating the cell layer, so that viruses generated by the cell layer penetrate through the multi-layer hydrogel layer. The chip system is helpful for reducing the phenomenon of cell shedding in the virus transfection process, and can improve the efficiency of transfecting viruses by cells.

This invention discloses a *Bacillus laterosporus* strain for the stable production of nonribosomal cyclic lipolipeptide and its construction method. The M1A gene in the Bogorol biosynthetic gene cluster is knocked out using CRISPR-Cas9 technology to block the Bogorol pathway, which competes with Brevicidine for substrates. Subsequently, the pMCCas9-S48 plasmid, which integrates S8 and S41 sgRNAs and their homologous arms as repair templates, is used to simultaneously knock out S8 and S41 family peptidase genes, preventing the expression of extracellular degradation enzymes. This invention also provides the application of the above strain in the preparation of nonribosomal cyclic lipolipeptide of Brevicidine and a method for preparing the nonribosomal cyclic lipolipeptide. This strain, through reducing metabolic competition and preventing product degradation, exhibits rapid cell growth, stable production of Brevicidine, and significantly reduced degradation rate, resulting in a 66.13% increase in fermentation yield compared to the unmodified strain. This invention solves the problems of degradation and unstable yield in the production of Brevicidine, provides an effective technical approach for the industrial production of Brevicidine, and provides important technical support for the development and application of novel antimicrobial peptide drugs.

The present invention relates to a peptide binding to OTUB1 and a use thereof. A peptide of a specific sequence having the property of binding to OTUB1 is observed to exhibit an anticancer effect on cancer cells without affecting the cell growth of normal cells, and thus can be usefully used as a composition for preventing, treating, or alleviating cancer diseases.

A device for safely and effectively disinfecting a catheter while it remains implanted in a patient's body is provided. [Solution] Methods and devices for providing therapeutic electromagnetic radiation (EMR) to inactivate infectious agents and / or enhance healthy cell growth in, on, or around a catheter (10) placed in a patient's body cavity. Non-ultraviolet therapeutic EMR is transmitted axially along the lumen of the catheter body and / or along an optical element (14) within the catheter body. Delivery of the therapeutic EMR to specific infected areas and / or areas requiring tissue healing results in inactivation of primary sources of infection in, on, and around the catheter and / or enhancement of healthy cell growth around the catheter through the use of controlled relative intensity and treatment-site-specific dose projection of the therapeutic EMR radially emitted from the optical element. Specific embodiments for urinary catheters and peritoneal dialysis catheters are also disclosed.

The use of an LtPCL6 protein and a gene encoding same in regulating pathogenicity, mycelial growth, and stress resistance of Lasiodiplodia theobromae. The gene encoding LtPCL6 protein is derived from Lasiodiplodia theobromae, and a knockout mutant is obtained by constructing a knockout fragment of a Lasiodiplodia theobromae LtPCL6 gene, and introducing same into a Lasiodiplodia theobromae protoplast. Experiments indicate that the LtPCL6 gene has an effect in the growth, cell wall and cell membrane integrity, and stress resistance of Lasiodiplodia theobromae, and significantly reduces the pathogenicity of the Lasiodiplodia theobromae with regard to a host. Therefore, the LtPCL6, serving as a fungicide target and a key protein of a pathogenic mechanism of diseases, can be used for developing agents for diseases caused by Lasiodiplodia theobromae infection and breeding new resistant varieties, and has broad application prospects in the control of plant pathogenic fungal diseases.

A transcatheter growth device for treating congenital heart disease in infants and other pediatric heart patients, and methods of making and using the same. The growth device includes an elongated device frame with alternating annular growth cell members and spacing members axially spanning the proximal and distal end regions of the device frame. The growth cell members and spacing members define the radial periphery of the growth device. Each growth cell member may include a scaffolded growth cell member having a first annular strut array and a second annular strut array connected via growth cell junctions to provide flexibility to the device frame. A retention member for engaging a selected lumen within the patient's body and a covering member for sealing the growth device upon deployment are disposed on the device periphery. The growth device can be advantageously deployed for the treatment of heart disease alone or in combination with one or more additional growth devices.

The invention discloses an improved culture medium containing egg yolk protein and a chicken embryo extract and a preparation method of the improved culture medium. The culture medium is mainly composed of egg yolk protein, a chicken embryo extract and a basic culture medium component according to a specific proportion. The improved culture medium is rich in various nutritional ingredients such as proteins, amino acids, vitamins and cell growth factors, can effectively promote the growth and proliferation of various cell lines and improve the efficiency and quality of cell culture, can be widely applied to the field of in-vitro culture of animal cells, provides a choice with higher quality and better cost effectiveness for cell culture, and has a wide application prospect. The problems that a traditional culture medium is insufficient in nutritional ingredients, poor in cell culture effect and the like are solved.

A method of inhibiting a cancer cell growth includes contacting the cancer cell with a Cu2(OH)3NO3 / CaSiO3@g-C3N4 nanocomposite material containing graphitic carbon nitride (g-C3N4), copper hydroxide nitrate (Cu2(OH)3NO3) and calcium silicate (CaSiO3), achieving an inhibition efficiency on human breast carcinoma (MCF-7) and human hepatocellular carcinoma (HepG-2) cell growth of greater than 95% in an in-vitro cellular viability assay.

The invention discloses a multilayer adherent cell culture reactor and a use method. The culture reactor comprises a shell, a plurality of layers of cell culture plates are arranged in the shell in an array manner; and the liquid inlet is used as an inlet of a cell suspension. The liquid outlet is an outlet of the culture medium; the air holes are formed in the upper surface and the lower surface of the shell. And the quality control chamber is communicated with the inner cavity of the shell. The use method comprises the following steps: uniformly mixed cells and a culture medium enter the inner cavity of the shell through the liquid inlet for positive culture; after front culture is completed, the multi-layer adherent cell culture device is inverted; uniformly mixed cells and a culture medium enter the inner cavity of the shell through the liquid inlet for reverse culture. By arranging the multiple layers of cell culture plates with double surfaces capable of culturing, the cell culture area is greatly increased, the space utilization rate is improved, the use amount of a culture medium is reduced, the prepared quality control chamber is communicated with the inner cavity of the shell, and the cell growth state is conveniently, rapidly and accurately observed on the premise of not polluting the internal environment and not damaging the culture stability through isolation of the partition opening.

A microcavity bioreactor is provided that allows cell growth and cell differentiation in the same vessel, such that higher order three-dimensional cell cultures such as organoids can be generated in a singular vessel. The microcavity bioreactor may be part of a microcavity bioreactor system that allows for perfusion based cell culture and perfusion based cell harvesting. The microcavity bioreactor includes at least one, and preferably at least two, fluid distributor structures in the housing vessel of the microcavity bioreactor.

The present invention generally pertains to methods of cultivating cells and producing a recombinant protein of interest, such as dupilumab. In particular, the present invention pertains to the use of a fixed seed schedule to determine the expansion time of seed train unit operations for improved cell growth and protein titer. The protein produced by the fixed seed schedule, such as dupilumab, can be later subjected to lipase activity reduction methods.

Provided herein are modified gelatins, cross-linked modified gelatins and hydrogels, and processes for making the same. Also provided herein is the use of such materials as matrices for cell growth.

The utility model provides a cell culture bottle, which relates to the technical field of cell culture and comprises a square bottle body, the inclined neck end of the bottle body is in threaded connection with a bottle cap, an adjusting structure is mounted on the outer side of the bottle body far away from the short edge of the bottle cap, and the height of the short edge of the bottle body is adjusted through the adjusting structure; the threaded adjusting groove and the sliding groove are formed in the two sides of the adjusting base, the threaded adjusting groove penetrates through the top end and the bottom, the sliding groove is internally provided with the penetrating bottom, the adjusting knob is in threaded connection with the interior of the threaded adjusting groove, the bottom of the adjusting knob is fixedly connected with the connecting base, and the movable groove is formed in the top end of the adjusting plate and corresponds to the connecting base. The connecting base is movably connected into the movable groove, the sliding rod is installed at the top end of the adjusting plate, and the upper end of the sliding rod is slidably connected into the sliding groove, through the arranged adjusting structure, the balance of the cell culture bottle on different inclined planes can be adjusted, cell sap is prevented from deviating to one side to be stacked, the stability of the cell culture environment is guaranteed, and cell growth is facilitated.

The invention discloses a construction method of a synovial sarcoma tumor ball vascular induction model, the model and application of the synovial sarcoma tumor ball vascular induction model. The construction method of the synovial sarcoma tumor sphere vascular induction model comprises the following steps: S1, suspension culture of synovial sarcoma organs; and S2, constructing a synovial sarcoma tumor ball vascular induction model based on the culture chip. When the synovial sarcoma organ is cultured, the synovial sarcoma primary tissue is cultured in a suspension culture mode, and the method not only can improve the proliferation speed of the synovial sarcoma organ, the cell growth state is better, the activity is higher, but also has the advantage of simplicity in operation. Besides, the synovial sarcoma tumor sphere vascular induction model is constructed based on the culture chip, and the model can simulate tumor microenvironment more truly, has tumor-related fibroblast and vascular components, and provides more clinical drug sensitivity test results.

An automated system for signaling cell growth events using continuous sensor data includes at least one sensor associated with a vessel configured for use in growth of cells, the sensor providing continuous sensor data associated with cell growth, a reader in operative communication with the sensor to collect the continuous sensor data, and a computational unit operatively connected to the reader, wherein the computational unit is configured to receive as input the continuous sensor data and calibration data associated with process conditions, the computational unit further configured to perform signal processing on the continuous sensor data in order to predict at least one of future critical events and generate insights about unexpected behavior.