846 results about "Growth cell" patented technology

The invention relates to the technical field of biological information, in particular to a stem cell culture information management method and system. The method comprises the following steps: collecting culture process basic data in a stem cell culture process, wherein the culture process basic data comprises cell counting data, culture condition parameter data and pollution condition data; performing growth kinetic analysis on the basic data in the culture process to obtain cell growth characteristic data; establishing a cell growth prediction model based on the cell growth characteristic data, and generating cell growth early warning data; and monitoring the culture process in real time according to the cell growth early warning data, triggering an early warning signal when abnormal fluctuation is detected, and generating a culture abnormality processing scheme. The growth prediction model is established based on the cell growth characteristic data, the culture dynamic state can be monitored in real time, an early warning signal is provided, the detection and response speed of abnormal fluctuation is remarkably improved, and culture failure is avoided.

The invention provides a virtual-real fusion method for digital twinning operation of a cell drug factory in combination with the Internet of Things, which comprises the following steps: constructing geometric models of a culture medium storage tank and a bioreactor through three-dimensional modeling software, mapping and associating historical process parameters of a culture medium supply system and temperature, pH value and dissolved oxygen concentration monitored in real time with the geometric models, and obtaining a virtual-real fusion model of the culture medium storage tank and the bioreactor; constructing a digital twinborn model of the reactor; analyzing historical production data through a machine learning algorithm, extracting a quantitative relationship among key parameters of a cell metabolism rate, a glucose concentration in a culture medium, a pH value and a reactor temperature, establishing a cell growth prediction model, and predicting a growth trend and a metabolism demand of cells in a target time period; a virtual-real mapping mechanism of a culture medium supply system is integrated with an intelligent manufacturing execution system, so that the culture medium supply process and the whole pharmaceutical production process are subjected to collaborative optimization control, and the working procedures of the culture medium production process are automatically dispatched according to a production plan and cell growth requirements.

The invention relates to the technical field of microbial fermentation, and discloses a genetically engineered bacterium of high-yield D-pantothenic acid as well as a preparation method and application of the genetically engineered bacterium. The D-PA yield of the engineering bacteria is greatly improved through the following effects that acetolactate synthase IlvB and IlvN shunted by pyruvic acid are subjected to overexpression, meanwhile, a transcription attenuation region is deleted, and valine feedback inhibition sites are mutated; the method comprises the following steps: overexpressing keto-alcohol acid reductoisomerase IlvC and dihydroxy acid dehydratase IlvD, and converting the IlvC coenzyme preference from NADPH into NADH, so as to break through the upstream of D-PA synthesis; overexpression of PanB is carried out, overexpression of a serine-glycine transport system is carried out, and synthesis of 5, 10-methylenetetrahydrofolic acid is promoted; meanwhile, the expression of ketopantoic acid reductase PanE is enhanced; and finally, over-expressing pantothenic acid synthetase PanC, and increasing the content of a cofactor ATP required by the PanC. According to the engineering bacteria provided by the invention, through overall reinforcement of the multiple modules, cell growth is improved due to balance of cofactors and increase of D-PA, and the yield of D-PA reaches an unprecedented height.

A method and system to make a modular microfluidic device in a miniaturized form for cell culture, co-culture, and organ-on-a-microfluidic device as in-vitro model of human organs within compartmentalized 3D structures is described. The modular microfluidic device enables co-culturing of heterogeneous cell assemblies that are adjacent or sequentially connected to promote enhanced cell-cell interaction. In vivo-like flow into and / or from one cell growth chamber to adjacent cell growth chamber is maintained by micro-engineered porous barriers i.e., porous walls and membranes. These porous barriers provide a tool for mimicking the paracrine exchange between cells in the human body. The system comprises of a set of microelectrodes for real-time and long-term monitoring and quantitative measurement of the impact of stimuli on the epithelial tissue permeability. The modular microfluidic system provides a platform technology for bio-fabrication of variety of miniaturized in-vitro models for mimicking the structure and function of single and multi-organ models.

The present invention relates to magnetic, catalytically active, and radiosensitizing nanoparticles (NanoXcats) and the method for their production. A method is disclosed for the production of functionalized greigite nanoparticles with a narrowband size distribution (10-30 nm) for use in multimodal cancer therapy. NanoXcats are superparamagnetic Fe3S4 nanoparticles whose surfaces are equipped with polar functional groups. The surface structures of NanoXcats are suitable for conjugation with, or for covalent or electrostatic binding of, active substances, which may be cancer drugs, cell growth inhibitors, or NO donors. Due to the EPR effect, NanoXcats are preferentially internalized by tumor cells. The targeted accumulation of NanoXcats in tumor tissue is achieved by an external inhomogeneous magnetic field. NanoXcats exhibit enhanced catalytic activity in tumor cells.The catalytic activity of intracellular NanoXcats can be triggered, controlled, and enhanced by low-dose X-rays. Surface-bound drugs are additionally activated in tumor cells by the catalytic activity of NanoXcats. NanoXcats act as superoxide dismutase, nitric oxide synthase, and Fenton catalyst. They catalyze, for example, reactions of the superoxide anion radical to hydrogen peroxide, of L-arginine to NO, and of hydrogen peroxide to the hydroxyl radical.

The invention provides a micromolecular multifunctional information peptide and application thereof, and belongs to the technical field of active peptide raw material development. The sequence of the small-molecule multifunctional information peptide is VHPYVPR, the small-molecule multifunctional information peptide can be effectively combined with a downstream receptor of EGF, the EGF function is simulated, and meanwhile the situation that use of existing cell growth factor raw materials in the field of cosmetics is limited due to the molecular weight problem is avoided. Experiments prove that the micromolecular multifunctional information peptide provided by the invention can promote the repair of skin damage phenomena such as scratches, inhibit the release of inflammatory factors, inhibit the activity of beta-galactosidase and promote the generation of I-type, III-type and XVII-type collagen, shows excellent repair, soothing and anti-aging functions, is high in permeability and has a wide application prospect.

The invention discloses a light-driven synthetic flora and application thereof. The light-driven synthetic flora comprises phototrophic bacteria and heterotrophic bacteria, the phototrophic bacteria are genetically modified synechococcus slender, and the gene modification comprises construction and expression of heterologous sucrose permease, glyceraldehyde dehydrogenase and sucrose synthase in the synechococcus slender; and the heterotrophic bacteria are the P.adami. According to the light-driven synthetic flora disclosed by the invention, carbon dioxide is converted into cane sugar through photosynthesis of phototrophic bacteria, and the cane sugar is secreted out of cells to provide a carbon source for heterotrophic bacteria; carrying out cell growth and polyhydroxyalkanoate synthesis on the heterotrophic bacteria by using cane sugar; therefore, the light-driven synthetic flora provided by the invention can realize direct conversion of carbon dioxide into polyhydroxyalkanoate without additional carbon sources, has the advantage of environmental friendliness, and provides a new economical and feasible scheme and approach for production of polyhydroxyalkanoate.

The invention provides a cell growth state detection method based on image segmentation, and belongs to the technical field of cell image processing and segmentation. Collecting a cell image in real time, and inputting the collected cell image into a hierarchical division feature encoder to obtain a cell block coarse-grained feature and a cell boundary fine-grained feature; inputting the cell block coarse-grained features and the cell boundary fine-grained features into a cascade feature fusion network to obtain deep fusion features; inputting the deep fusion feature into a decoder to obtain a cell image instance segmentation result; and calculating the number and survival rate of the target cells according to the cell image instance segmentation result. The newly designed image extraction and segmentation network is combined with a double-task cooperative training strategy, a cell image instance segmentation task and a cell image semantic segmentation task are combined, real-time detection of the cell growth state is achieved, time consumption and low efficiency of manual observation in a traditional method are avoided, and the method is suitable for real-time detection of the cell growth state. And pollution and damage possibly caused by manual sampling are avoided.

The current invention provides a biocompatible energy recharge system to normalize body functions for a mammal. Both stationary and circulatory scalar wave energy was produced and utilized to enable the entire body of a mammal to increase the energy potential of any cell in the body, to promote cell growth, to facilitate cell repair, and to reduce cell destruction, hence the cells, organs, systems and the entire body of a mammal become healthy, and to treat a disease or disorder. The invention is a combination of the generators of the three-dimensional bio-waves in the stationary and in the circulationary formats for empowering the cells in all parts of a mammal, and a method of using the 3D bio-waves uniformly to empower each organ of a mammal to restore and maintain the normal body health and function.

The invention provides a cell growth dynamics prediction method and device based on a space-time image sequence, and belongs to the technical field of computer vision and deep learning. The method comprises the following steps: acquiring a multi-focal-plane space-time image sequence containing a cell complete growth process; preprocessing and normalizing images in the image sequence to obtain a final test image sequence; and inputting the test image sequence into a cell growth dynamics prediction model consisting of a multi-scale feature extraction module and a time sequence modeling module which are connected in sequence to obtain a cell growth stage identification result corresponding to each time point image in the sequence so as to realize cell growth dynamics prediction. The method can effectively solve the problems of poor adaptability, insufficient stability, high labeling cost and the like when a traditional model is used for processing the dynamic medical image sequence, and has remarkable advantages and application prospects in the aspect of dynamic prediction of the cell growth space-time image sequence.

The invention discloses a verification method for regulating and controlling proliferation and differentiation of bovine adipocytes by gene interference, which comprises the following steps: cutting bovine adipocyte tissues into small blocks, directly pasting the small blocks in a culture dish for culture, and promoting cell growth and proliferation by using nutrition and growth factors of the tissues; a ZNF423 gene is targeted by adopting an siRNA technology, siRNA is transfected into bovine fat cells by utilizing a transfection reagent, and the siRNA is an interference fragment containing si-ZNF423; the method comprises the following steps: respectively extracting total RNA (Ribonucleic Acid) and total protein of bovine adipocytes, and detecting by utilizing qPCR (Quantitative Polymerase Chain Reaction) quantitative analysis, a WB (White Batch) technology, EdU dyeing, flow cytometry detection, oil red O, BODIPY (Boron Dipyrromethene), Nile Red dyeing, TG (Triglyceride) content determination and the like. According to the invention, ZNF423 gene expression is interfered by the interference fragment containing si-ZNF423, and the effect of inhibiting proliferation and differentiation of bovine adipocytes is detected by various methods, so that the effect of the interference fragment in the bovine adipocytes is known conveniently, and regulation and control of proliferation and differentiation of the bovine adipocytes are realized by interfering gene expression by the interference fragment. The beef quality can be improved; the beef cattle breeding can be optimized.

The invention relates to a triple-hole paper-based three-dimensional cell culture chip as well as a preparation method and application thereof. The chip comprises a paraffin hydrophobic region and a paper-based hydrophilic region. The preparation method mainly comprises the following steps: 1) designing a mask plate; 2) cutting the mask plate by laser; and 3) preparing the triple-hole paper-based chip by hot pressing. The thickness of the chip is 100-200 [mu] m, the porous fiber structure of the paper-based material allows transportation of oxygen and waste, and a three-dimensional microenvironment for in-vivo cell growth can be simulated. Meanwhile, a transparent and liquid-tight supporting layer is fused on the lower layer of the paper-based chip, so that leakage of cells inoculated on the upper layer of the paper-based chip is avoided. The paper-based chip is embedded into a 6-pore plate, and bladder cancer cells (5637), mouse astrocytoma (U87) and human breast cancer cells (MCF-7) are inoculated in a paper-based hydrophilic region respectively. The cell compatibility of the paper-based chip and the growth behaviors of three different types of cells in three-dimensional paper fibers are investigated. It is proved that the chip can be used for researching three-dimensional construction and growth of human and mammalian cells, and the difference between three-dimensional culture and two-dimensional culture is revealed by detecting the proliferation capacity and survival rate of different cells.

The invention discloses a pharmaceutical composition for promoting aTreg cell differentiation and application thereof, and relates to the field of biological medicine, and the pharmaceutical composition comprises zinc salt, a basic cell culture medium, fetal calf serum, antibiotic, L-glutamine and a pH buffer agent. According to the application, the basic cell culture medium is used for providing basic nutrition required by cell growth for lymphocytes, the fetal calf serum is used for providing growth factors, hormones, adhesion proteins and nutrient substances required by cell growth for the lymphocytes, and the antibiotics are used for preventing bacterial infection; l-glutamine provides key amino acid for cell energy metabolism and participates in synthesis of protein and nucleic acid, zinc salt provides zinc ions, and under the comprehensive action, the proportion of Treg cells and aTreg cells differentiated from lymphocytes in peripheral blood mononuclear cells is increased.

The present invention relates to the technical field of biopharmaceutical apparatuses, in particular to a sterile culture method and a sterile culture system. The sterile culture system comprises a cell preparation device, an incubator, and a cell culture device; the cell preparation device comprises a preparation chamber, wherein a first sealing valve is arranged on a side wall of the preparation chamber; the incubator has a culture cavity, wherein a second sealing valve is arranged on a side wall of the culture cavity, and the second sealing valve is configured to work with the first sealing valve such that the incubator is hermetically docked with or separated from the cell preparation device; and the cell culture device is used for accommodating the incubator, supplying power and a gas to the incubator, and controlling a cell growth environment in the incubator. Because cells grow in a sterile environment all the time and the pollution risk is relatively low, no frequent sterilization is required. Normal growth of cells is avoided from being affected by frequent sterilization, and the culture time is saved. Therefore, the cell preparation and culture efficiency is relatively high.

The invention relates to the technical field of cell culture media, in particular to a marine invertebrate cell culture medium. Comprising the following components in parts by weight: 0.5 to 1 part of a compound supplement, 1 to 4 parts of glucose, 0.3 to 0.5 part of L-glutamine, 0.01 to 0.05 part of vitamin, 10 to 15 parts of fetal calf serum, 0.02 to 0.04 part of penicillin, 0.01 to 0.03 part of trehalose, 0.3 to 0.7 part of a sodium chloride osmotic pressure regulator, 0.5 to 1 part of inorganic salt and 10 to 25 parts of a sodium bicarbonate buffer agent, wherein the compound supplement is prepared by mixing meson, diatom and disodium cyclohexane diamine tetraacetate according to the mass ratio of 1: (0.2-0.6): (0.1-0.5), the application range of the culture medium is widened by adopting the compound supplement, the meson and the diatom are added into the cell culture medium after enzymolysis, and compared with a chemically synthesized culture medium, the culture medium better meets the requirements of marine invertebrate cells; therefore, cell growth is effectively promoted, and the purposes of rapid growth and stable passage are achieved.

The invention discloses a biological composite material as well as a preparation method and application thereof. The biological composite material comprises a decellularized biological amnion, mesenchymal stem cells and polypeptide hydrogel. In the polypeptide hydrogel, the sequence of the polypeptide is IGSRVGDRGDS. The biological composite material can effectively promote cell growth at a thin endometrial injury position, endometrial cell proliferation activity is higher, new vessel production related gene regulation activity is enhanced, fibrosis caused by the endometrial injury is well inhibited, re-adhesion after an intrauterine adhesion separation operation is prevented, and a good application prospect is achieved. The fertility is obviously improved, and the pregnancy rate is increased.

The invention discloses a heterocyclic compound and application thereof. The invention provides a compound as shown in a formula (I), a tautomer thereof, a pharmaceutically acceptable salt thereof, a solvate thereof or a solvate of the pharmaceutically acceptable salt thereof. The heterocyclic compound provided by the invention shows a good cell growth inhibition effect on tumor cell strains of human breast cancer cells HCC1569 and human ovarian cancer cells OVCAR3, and the compound provided by the invention can be used as a PKMYT1 inhibitor. # imgabs0 #

The invention relates to the field of microorganisms, and discloses lactobacillus rhamnosus and application thereof. The lactobacillus rhamnosus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.33153, provided by the invention, has relatively strong reproductive capacity, can produce lactic acid and hydrogen peroxide at high yield, and has relatively strong antibacterial performance; the polypeptide has the potential of being applied to organisms; the composition can regulate and control inflammation and apoptosis related genes, so that the generation amount of inflammatory factors is reduced, cell growth and repair are promoted, and gene expression of aquaporin and collagen can be up-regulated, so that skin repair is promoted.

The invention discloses a preparation method of an in-situ gelation collagen-based biological patch, which comprises the following steps: generating hydrogel in situ on the surface of an acellular matrix material and among internal fiber gaps by introducing various chemical reactivity of dopamine / polydopamine, and combining a hydrogel system and the acellular matrix material into one to construct the in-situ gelation biological patch. The material effectively improves the mechanical support effect, drug loading and cell growth promotion of defective tissues, also has the tissue repair functions of healing promotion, adhesion prevention, infection resistance and the like, and can be widely used for functional modification of acellular matrix materials.

A device and method for assembling objects by generating an axial acoustic radiation force in order to position the objects on pressure nodes and / or antinodes and generating a transverse acoustic radiation force in order to move the thus positioned objects on a porous membrane contact structure.

Disclosed in the present invention are a gel having a regulatable free water content and morphology, a preparation method therefor, and the use thereof. The method comprises: S1, preparing a solution A from a natural polymer material; S2, mixing a small molecule material containing a positively charged polar group with the solution A to obtain a composite solution B; S3, mixing the composite solution B with a regulator to obtain a composite solution C, stirring same, then adding a condensing agent to the reaction system, and carrying out a curing crosslinking reaction; and S4, removing the regulator and the condensing agent to obtain the gel. By adjusting the concentrations of the natural polymer material and the regulator, gels having different morphologies can be prepared; the gel undergoes a physical process to remove hydrogen bond interaction between the gel and free water, so that the content of free water attached to the surface of the gel can be regulated, thereby regulating the physical and chemical properties of the gel. The gel of the present invention has regulatable morphology and controllable elastic modulus, and has many advantages such as good lubrication effect, good biocompatibility and biodegradability, capability of being biologically active, and capabilities of stimulating cell growth and accelerating wound repair.

The present invention discloses a personalized customization of cell-cultured meat based on a porous scaffold material and a production method thereof. The method adopts a material suitable for cell growth and safe and edible, prepares a porous scaffold material with a suitable pore structure based on freeze-drying, and realizes personalized customization of cell-cultured meat by adjusting the cell inoculation parameters. The porous scaffold material constructed by the present invention has good biocompatibility and mechanical properties. Its high porosity, connectivity and suitable pore size structure are suitable for the intercellular information transmission and sufficient exchange of nutrients during cell culture, which is beneficial to the fate processes such as cell growth, proliferation, differentiation and maturation. In addition, the edible qualities such as texture, taste and nutritional value of cell-cultured meat can be improved by inoculating different types of cells and changing the cell inoculation ratio, thereby realizing personalized customization of cell-cultured meat. The porous scaffold material provided by the present invention has important guiding significance for the personalized development of the cell-cultured meat production industry.

The present invention relates to the field of bioengineering technology and provides a sucC mutant and its application in the fermentative production of L-valine. By performing point mutations on the sucC gene, the present invention reduces the enzyme expression activity, balances the metabolic flux problem between the TCA cycle for cell growth and product accumulation, and thus improves the valine yield. Further, through experimental screening, a recombinant strain capable of significantly increasing the valine yield is obtained. From the fermentation results, it can be seen that this method can significantly increase the valine yield, from the original 1.3 g / L to 1.61 g / L, with a 23.8% increase in yield, providing strong strain support for further industrialization.

Porous-based bone implants with the integration of Triply Periodic Minimal Surface, TPMS, porous architectures are designed to support the growth and proliferation of bone tissue, bone marrow, and capillaries. This disclosure intends to reduce the adverse effect of conventional implants such as bone resorption over time, which is called the “stress shielding effect”. The “stress shielding effect” is caused by the mismatch between the implant and natural bone stiffness. Triply Periodic Minimal Surface, TPMS, porous architectures exhibit interconnected pore features. The interconnection of the porous network allows the TPMS to have a higher permeability than that of other porous structures, leading to more favorable nutrient transport. In addition, many physical characteristics of the TPMS structures including surface-to-volume ratio, pore size, elastic modulus, and fluid behaviors can be controlled precisely through mathematical manipulation. As a result, TPMS-based implants could be physical features, which are vaned based on different bone regions. In other words, the medical implants may exhibit non-uniform or gradient physical features, which can match the characteristic of trabecular and cortical bones. Therefore, TPMS-based implants could adjust the features to mimic neighboring bone regions. As a result, we can achieve medical implants, which have superior mechanical and biological responses, resulting in optimal cell growth and better medical treatment.

The invention relates to the technical field of medical gel, in particular to gynecological vaginal mucosa repairing gel and a preparation method thereof. Comprising the following raw materials: carbomer, glycerol, sodium hyaluronate, vitamin E, potassium sorbate, a modified nano polylactic acid-glycolic acid copolymer, modified nano collagen, albumin, tea tree essential oil, aloe, astragalus membranaceus, dandelion, stigma croci and ginseng. The modified nano polylactic acid-glycolic acid copolymer, the modified nano collagen and the albumin play a role through a synergistic effect, and the modified nano polylactic acid-glycolic acid is used as a skeleton structure for cell growth and is firmly attached to the active pharmaceutical ingredient-albumin compound; the polyacrylic acid chain on the surface controls the drug release rate by sensing the change of the pH value, the albumin initiates endocytosis through specific binding, and the albumin and the modified nano collagen jointly improve the anti-inflammatory performance, promote cell proliferation and differentiation and promote repair and regeneration of vaginal mucosa tissues.

The invention belongs to the technical field of microbial engineering and genetic engineering, and discloses escherichia coli for producing histidine as well as a construction method and application thereof. The escherichia coli contains a growth dependent promoter PprpB of a methyl isocitrate lyase gene prpB and / or a stable phase dependent promoter PydgI of an ornithine reverse transport protein / lysine permease gene ydgI. Escherichia coli for producing histidine uses growth dependent promoter PprpB of methylisocitrate lyase gene prpB to regulate expression of glutamine phosphoribose pyrophosphate transamidase gene purF and uses ornithine reverse transport protein / lysine permease gene (ydgI) The stable-phase dependent promoter PydgI regulates the expression of the ATP phosphoribosyltransferase gene hisG, so that the energy supply of thalli in early-stage cell growth and the metabolic flux of L-histidine in later-stage thalli production can be realized, and the balanced distribution of the metabolic flux between the cell growth of a microbial cell factory and the production of L-histidine is effectively balanced.

The invention discloses an anisotropic electrostatic spinning patch with ultrasonic response and a preparation method thereof, a fiber membrane with a controllable anisotropic structure is constructed by a biodegradable polymer, antibacterial nanoparticles and a photoinitiator system through a step-by-step directional electrostatic spinning process, so that the mechanical matching property of the fiber membrane is improved; cell growth can be directionally guided and tissue regeneration can be accelerated by combining the piezoelectric material characteristics of the introduced biodegradable polymer with piezoelectric characteristics and electric field stimulation induced by the fiber topological structure; meanwhile, a zwitterionic coating is generated on the surface of the fiber membrane in situ by utilizing a single-sided photocuring technology, and an anti-adhesion function is realized on the single side of the fiber membrane. Furthermore, the soft tissue repair patch which has multiple functions of adjustable mechanical property, infection resistance, adhesion resistance and the like and has better structural mechanical suitability, function integration and tissue regeneration guiding capability is prepared by adopting the anisotropic electrostatic spinning patch with ultrasonic response.

The invention relates to a preparation method and application of a functional gradient layered bone-imitating GBR (Guarantee Boron Reactor) membrane, the GBR membrane is a novel GBR membrane obtained by taking decellularized fish scales as a base material, constructing an EGCG-fish scale complex through EGCG cross-linking modification and then combining biomimetic mineralization, and the GBR membrane has certain mechanical properties and can maintain a space required by bone regeneration; meanwhile, mineralized hydroxyapatite wraps fish scale collagen, so that the degradation speed of fish scales is reduced, the action time is prolonged, and sufficient time is provided for bone repair; in addition, the mineralized fish scales are similar to natural cortical bones in structure and composition, a cell growth microenvironment is simulated, good biocompatibility is achieved, adhesion and differentiation of osteoblasts are promoted, and the healing speed is increased.

The invention discloses a method for designing a bionic bone scaffold based on wall thickness mixed gradient change, and belongs to the technical field of bionic bone scaffolds. By designing the wall thickness mixed gradient function, the lattice unit can have wall thickness gradient change in the radial direction and the axial direction at the same time, so that the porosity also has gradient change in three directions at the same time, and the defect that a bone scaffold is designed by using a lattice material (beam unit) unit and a TPMS unit in the prior art can be well overcome; and the stent better conforms to the natural bone structure and mechanical property requirements. The designed bionic bone scaffold is excellent in mechanical property, high in compression resistance and deformation resistance and capable of promoting cell growth and tissue regeneration.

The invention discloses a method for establishing a cell line by serum-free culture of PGCs (Primordial Germ Cells) of breeding poultry, the cell line is prepared by blending calcium-free DMEM (Dulbecco Modified Eagle Medium), water and Knockout-DMEM, the cell line is more suitable for osmotic pressure and calcium ion concentration required by proliferation of the PGCs of the breeding poultry, various components of serum-free culture are perfected by using fresh egg white, and the cell line is more suitable for proliferation of the PGCs of the breeding poultry. The combination of growth factors required by primordial germ cells is optimized by adding the FGF-1, so that 100% of cell line establishment efficiency and rapid growth of cells from reproduction crescent, blood and gonad tissues of chicken embryos are realized, and a technical basis is provided for poultry frozen breed conservation, stem cell propagation and gene editing operation.