87results about How to "Promote cell adhesion" patented technology

The present invention relates to a biocompatible, three-dimensional scaffold useful to grow cells and to regenerate or repair tissue in predetermined orientations. The scaffold is particularly useful for regeneration and repair of cardiac tissue. The scaffold contains layers of alternating A-strips and S-strips, wherein the A-strips within each layer are aligned parallel to each other and preferentially promote cellular attachment over attachment to the S-strips. Methods of producing and implanting the scaffold are also provided.

A product exhibiting an improved cell growth surface and cell attachment. According to the present invention, a stream of plasma is comprised of activated gaseous species generated by a microwave source. This stream is directed at the surface of a polymer substrate in a controlled fashion such that the surface is imparted with attributes for cell adhesion far superior to that of untreated polymer or polymer treated by other methods.

The present invention claims and discloses a novel apparatus and method for efficiently cultivating cells with minimal mortality in order to harvest a maximum amount of cellular products generated by the cultivated cells. More particularly, the present invention teaches a method and a device for plating cells and causing maximum adherence of cells of interest. Furthermore, the present invention also teaches a growth substrate means that is capable of providing the largest surface area for cell adhesion and functions as an oxygenator, a depth filter and a static mixer to maximize the production of cellular products by intermittently and periodically provide sufficient oxygen and nutrients to the cells without causing cell death. The device of the present invention is economical and can be disposable thus eliminating complications caused by sterlization and is capable of periodically and intermittently provide oxygen and nutrients to cells, through controlling the amount of culture medium that comes into contact with the growth substrate means.

Engineered peptides that bind with high affinity (low equilibrium dissociation constant (Kd)) to the cell surface receptors of fibronectin (α5β1) or vitronectin (αvβ3 and αvβ5 integrins) are disclosed. These peptides are based on a molecular scaffold into which a subsequence containing the RGD integrin-binding motif has been inserted. The subsequence (RGD mimic) comprises about 9-13 amino acids, and the RGD contained within the subsequence can be flanked by a variety of amino acids, the sequence of which was determined by sequential rounds of selection (in vitro evolution). The molecular scaffold is preferably based on a knottin, e.g., EETI (Trypsin inhibitor 2 (Trypsin inhibitor II) (EETI-II) [Ecballium elaterium (Jumping cucumber)], AgRP (Agouti-related protein), and Agatoxin IVB, which peptides have a rigidly defined three-dimensional conformation. It is demonstrated that EETI tolerates mutations in other loops and that the present peptides may be used as imaging agents.

The present invention relates to a biocompatible, three-dimensional scaffold useful to grow cells and to regenerate or repair tissue in predetermined orientations. The scaffold is particularly useful for regeneration and repair of cardiac tissue. The scaffold contains layers of alternating A-strips and S-strips, wherein the A-strips within each layer are aligned parallel to each other and preferentially promote cellular attachment over attachment to the S-strips. Methods of producing and implanting the scaffold are also provided.

The present invention relates to a polymerization-curable composition for the preparation of biodegradable, biocompatible, cross-linked polymers on the basis of polyvinyl alcohol comprising: 5 to 100% by weight of (a) vinyl ester monomer(s) of one of the general formulas (I) to (III):wherein X is oxygen, sulfur, nitrogen, or phosphorus; n is 1 to 1000, at least 20% of the n being ≧2; the R1 are selected from hydrogen; straight, branched or cyclic, saturated or unsaturated, n-valent hydrocarbon groups having 1 to 30 carbon atoms, which optionally have heteroatoms and are optionally substituted with one or more substituents selected from —OH, —COON, —CN, —CHO, and ═O, and n-valent radicals of biodegradable, biocompatible oligomers and polymers; m is an integer from 1 to 5; the R2 are selected from hydrogen, —OH, ═O, and the options listed for R1; and the R3 are selected from hydrogen, —OH, and the options listed for R1; 0 to 50% by weight of ethylenically unsaturated co-monomers; 0 to 10% by weight of (a) polymerization initiator(s); and 0 to 95% by weight of solvent(s).

The present invention provides a novel substrate for use in growing cells and for the study of mechanobiology. The membrane of the present invention comprises appropriate microtopography and surface chemical modifications to facilitate the production of adherent and oriented cells that phenotypically resemble cells in vivo.

Novel compounds having activity as non-covalent inhibitors of urokinase and having activity in reducing or inhibiting blood vessel formation are provided. These compounds have Pi a group having an amidino or guanidino moiety or derivative thereof. These compounds are useful in vitro for monitoring plasminogen activator levels and in vivo in treatment of conditions which are ameliorated by inhibition of or decreased activity of urokinase and in treating pathologic conditions wherein blood vessel formation is related to a pathologic condition.

The present invention provides bionanocomposite materials comprising at least two coaxial layers of bionanocomposites, and methods for producing and using the same. The bionanocomposite materials of the present invention comprise a core structure and a shell structure encapsulating the core structure, where one of the core structure or the shell structure comprises a porous biocompatible natural-derived material and the other comprises a biocompatible biomimetic nanostructure.

A high strength surgical material with improved tie down characteristics and tissue compliance formed of ultrahigh molecular weight polyethylene (UHMWPE) yarns, the suture being coated with arginine-glycine-aspartate (RGD) peptide. The suture has exceptional strength, is ideally suited for most orthopedic procedures, and can be attached to a suture anchor or a curved needle.

A UV-cross-linkable PVA-based polymer coating for cell culture that provides support for cell adhesion. The polymer coating may also contain bioaffecting molecules reversibly entrapped within the polymer coating that provides necessary nutrients to cell culture. Preferably, the UV-cross-linkable PVA-based polymer is PVA-SbQ.

The invention relates to the preparation of a hydroxyapatite-grafted polylactide / polylactic acid-copolymerized glycolic acid electrospun nano-fiber-based guided bone regeneration membrane. The membrane is prepared from a mixture of HA-g-PLLA (hydroxyapatite-grafted poly-L-lactic acid) nanoparticles and PLGA (poly(L-co-glycolic acid) by an electrospinning method, i.e., the novel biodegradable guided bone regeneration membrane is constructed. Compared with PLGA and HAP / PLA fiber membranes, the prepared HA-g-PLLA / PLGA composite fiber membrane has the advantages that high mechanical performance is achieved, the adhesion and ductility of osteoblasts on the surface of the membrane are superior to those of the other composite membranes, and the HA-g-PLLA / PLGA composite fiber membrane has a broad application prospect in the treatment of guided bone regeneration.

An object of the present invention is to provide a cell culture support making the detachment of a cell sheet easy as well as enabling the formation of a uniform cell sheet. The present invention relates to a method for manufacturing a cell culture support having a temperature responsive polymer immobilized onto the surface thereof via covalent bonding, the method including a coating step in which a composition including a monomer that can form the polymer by polymerization by radiation irradiation, an organic solvent and, in some cases, a prepolymer formed by polymerization of the monomer is coated onto the substrate having a surface containing a material which can be covalently bonded to the temperature responsive polymer by radiation irradiation to form a film on the surface of the substrate, a radiation irradiation step in which a polymerization reaction and a binding reaction between the substrate surface and the temperature responsive polymer are allowed to proceed by irradiating radiation to the film, and a drying step to dry the film.

Substrates for influencing the organization, spreading or adhesion of a selected cell to induce or stimulate growth, differentiation or regeneration of the cell or of tissue constituting the cells are provided as well as methods of making such substrates and methods of using such substrates.

The invention discloses a recombinant I-type humanized collagen polypeptide as well as a preparation method and application thereof. The recombinant I-type humanized collagen polypeptide provided by the invention comprises n repeats of a sequence shown in SEQ ID No. 1, wherein n is an integer greater than or equal to 1, and when n is an integer greater than or equal to 2, the repeat sequences are directly connected; and optionally, the N tail end of the recombinant I-type humanized collagen polypeptide comprises an amino acid sequence which can be excised by TEV protease. The recombinant I-type humanized collagen polypeptide provided by the invention has the activity of promoting cell adhesion, the amino acid sequence of the recombinant protein is selected from a natural collagen amino acid sequence, and the recombinant protein does not generate an immune response when being applied to a human body; and moreover, the preparation method is simple, and high-yield collagen can be obtained at low cost.

The invention provides a preparation method of a polydopamine / hyaluronic acid modified coating polylactic acid membrane. The method comprises the following steps: with polylactic acid (PLA) as a base material, coating the PLA surface with a layer of polydopamine coating for modification to obtain PDA / PLA based on the characteristic that the dopamine (DA) can be adhered to the surfaces of multiple materials; and performing further surface modification on the modified PDA / PLA base material through using DA grafted hyaluronic acid (HA-DA) prepared in advance to obtain a HA-DA / PDA / PLA composite material. The composite material prepared by the method provided by the invention has good degradability, cell adhesion and biocompatibility, and has a broad application prospect in the fields such as bone repair and packaging.

The present invention relates to a polypeptide, a production method and uses thereof, wherein the polypeptide comprises an N-terminal region and a C-terminal region, and has remarkable cell adhesion effect.

A method for preparing a sericin hydrogel, the method including: 1) weighing a cocoon of a fibroin-deficient mutant silkworm, Bombyx mori, extracting the cocoon by an aqueous solution of LiBr or LiCl, dialyzing an extracted solution to yield a sericin solution having a concentration of a non-degraded sericin of between 0.1 and 4 wt. %; and 2) concentrating the sericin solution to a concentration of between 1.5 and 10 wt. %, adding a crosslinking agent to the concentrated sericin solution at a ratio of between 2 and 500 μL of the crosslinking agent per each milliliter of the sericin solution, fully blending the crosslinking agent with the concentrated sericin solution, and keeping a resulting mixture at the temperature of between 4 and 45° C. for between 5 s and 36 hrs to yield a hydrogel.

Novel inhibitors of urokinase are provided which have an arginine or arginine mimic aldehyde or an arginine ketoamide group at P1. These compounds are useful in vitro for monitoring plasminogen activator levels and in vivo in treatment of conditions which are ameliorated by inhibition of or decreased activity of urokinase.

The invention provides a preparation method of a DA-based gradient functional material. DA serves as a functional group, and the chemical reactivity, oxidation auto-polymerization and adhesion of DA are utilized to prepare the functional material with a multistage pore channel and a gradient structure. The method includes the steps that firstly, Alg is subjected to graft modification with DA to prepare biologically-modified macromolecular Alg-DA with excellent adhesion performance; then, DA is subjected to oxidation auto-polymerization assembly in a slightly alkaline buffer solution to form PDA particles of a uniform particle size; then, Alg-DA of different concentrations and the PDA particles act on each other to form a first-stage cross-linked structure, and then the gradient functional material is prepared with a lamination freeze-drying method; then, the gradient functional material is cross-linked again with calcium ions, and the cross-linking degree and porosity of the material are further adjusted. The DA-based gradient functional material provides a new way for preparing composites with an ideal texture. The gradient functional material prepared through the method has high mechanical performance and excellent biocompatibility and biodegradability, can effectively improve the absorption separation performance and the regeneration performance of soft / hard tissue and has broad application prospects in the fields of multifunctional separation and absorption films and biomedicine.

The invention discloses a preparation method of an intra-fibrous biomimetic mineralized collagen membrane capable of promoting osteogenic differentiation of cells. According to the invention, the preparation method comprises the following steps: preparing a collagen solution; pouring the collagen solution into a prefabricated mold, putting the mold into a closed container containing ammonia water,carrying out neutralizing, moving the neutralized solution out and then putting the solution into a constant-temperature box to carry out culturing continuously, and completing self-assembling and forming a gel mode; carrying out cross-linking on the gel to obtain pure collagen gel; and putting the pure collagen gel into a biomimetic mineralization solution and carrying out culturing for a plurality of days to obtain collagen gel with different biomimetic mineralization degrees; and cooling the collagen gel and carrying out freeze-drying on the cooled gel in a freeze dryer to obtain the finalintra-fibrous biomimetic mineralized collagen membrane. According to the invention, intra-fibrous mineralization of the collagen fiber can be formed by biomimetic mineralization; the fiber network structure of the collagen membrane is reserved and utilized while the mechanical property and the biological property of the hydroxyapatite crystal are utilized.

The invention discloses an agricultural microbial agent microcapsule based on cell membrane mimicking and a preparation method thereof. The effect of regulating the osmotic pressure of cells is realized by regulating the moisture content of microbial cells and the flux of oxygen; and the wall material of the microcapsule is selected from gamma-polyglutamic acid (gamma-PGA), a polysaccharide material and dopamine. The gamma-PGA can achieve a synergistic effect with a microbial agent so as to enhance the antioxidant ability of plants, alleviate damage to plants under abiotic stress and promote plant growth, and is a typical microorganism-derived biostimulant; and a compact film formed by auto-polymerization of the dopamine on the surfaces of bacteria can effectively relieve the influence ofexternal osmotic pressure on the bacteria in the microcapsule, and polymerization reaction conditions are mild and have small damage to the bacteria. The preparation method disclosed by the inventionis based on a cell surface polymerization technology, and can realize single-cell coating of the microbial agent and inhibit proliferation of the microbial agent.

The invention belongs to the technical field of wound dressings, in particular to a core-shell drug-loaded nano-fiber dressing and a preparation method thereof. The invention provides the core-shell drug-loaded nano-fiber dressing, and the core-shell drug-loaded nano-fiber dressing is obtained through coaxial electrostatic spinning, and sustained release of drugs can be realized; a core layer is constituted by mel and polyvinyl alcohol, and a shell layer is constituted by epsilon-polylysine and polycaprolactone; due to a core-shell structure, the mel as a natural active substance can be effectively protected, an influence of an organic solvent on the active substance is avoided; through wrapping with polycaprolactone as a hydrophobic shell layer, the phenomenon that glutaraldehyde as a toxic and organic solvent is crosslinked with the polyvinyl alcohol can be avoided; the epsilon-polylysine is loaded on the shell layer, so that a good bacteria inhibition effect is achieved; and moreover, the epsilon-polylysine also can enhance interaction between cells and materials, is beneficial for adhesion and proliferation of the cells, and is high in biocompatibility.

The invention discloses a dual-phase magnetic nano-composite scaffold material. The dual-phase magnetic nano-composite scaffold material is prepared by compositing a cartilago phase with a bone phase,wherein the cartilago phase contains polylactic acid and a natural polymer compound; and the bone phase contains polylactic acid, nano-hydroxyapatite and magnetic nanoparticles. The invention furtherdiscloses a preparation method of the dual-phase magnetic nano-composite scaffold material. The three-dimensional dual-phase magnetic nano-composite scaffold material is prepared from polylactic acid, the natural polymer compound, nano-hydroxyapatite and the magnetic nano particles by virtue of a low-temperature rapid forming technique and is integrated with the advantages of the four materials,is capable of promoting the adhesion and proliferation of cells, reducing the toxicity of degradation products and improving the biomechanical properties based on good osteoconduction and biocompatibility and is relatively beneficial to the adhesion growth and vascularization of solid cells, and the speed and effect of the coalescence between artificial cartilages transplanted at bone defect partsof a joint cartilage and a subchondral bone and the bones are greatly increased and improved.

Substrates for cell culture and tissue engineering bioreactors consisting of polymers that change their shape over time under stimulation by temperature change, hydration, degradation, or other means. A method of controlling cell culture using a biodegradable shape memory polymer, wherein shape changes can transfer stresses, strains, or both to adherent or otherwise connected cells such that the mechanical stimulus impacts cell development and the resulting properties of tissues.