55 results about "Joint cartilage" patented technology

The application provides a composite gel product which is designed according to the main components of articular cartilage based on the bionics principle and is made of biological cellulose (BC), polyvinyl alcohol (PVA), an anionic surfactant and chitosan, has mechanical properties, friction characteristics close to articular cartilage and good biocompatibility and degradability and can be used for preparing a cartilage joint injury repair material.

A drill-less method of stabilizing a sacroiliac joint using a multimodal abrading device to create a rich environment for bony fusion. The unique decortication instrument includes an integral slap hammer assembly joined with a rasp-box osteotome tooling head to simultaneously remove cartilage from the joint while preparing the sacrum and ilium down to bleeding bone. Subsequent to joint preparation, an implant with a large bone graft window may be implanted into the prepared SI joint through a specially designed access cannula.

Devices and methods for treating joint degeneration (e.g., intervertebral disc degeneration) are provided. An example method may comprise: (1) advancing a tip of an introducer needle into a nucleus pulpous of an intervertebral disc; (2) with the tip of the introducer needle in the nucleus pulpous, maneuvering, out of the tip of the introducer needle, a tip of a flexible needle into a region of a cartilaginous endplate (CEP) of the intervertebral disc; and (3) delivering, from an aperture of the flexible needle, therapeutic to the region of the CEP.

The application discloses a functional composition for improving bone and joint health, a conditioner and application. The functional composition comprises a first active component and a second active component; the first active component comprises elastin peptide, haematococcus pluvialis and ergothioneine; and the second active component comprises one or more of houttuynia cordata and non-denatured type II collagen. The application realizes the protection effect on joint, cartilage and bone health by scientific matching of the components and synergistically improving the glycosaminoglycan level in chondrocytes and the osteocalcin level in osteoblasts. Meanwhile, the components in the functional composition for improving bone and joint health are natural and have good biocompatibility, and have no obvious side effects, so that the application provides a kind of drug or a substitute for the treatment and prevention of bone diseases.

The application discloses a joint cartilage stress dynamic monitoring system based on flexible sensing and AI and belongs to the technical field of medical health monitoring.The application solves the problem that the prior art can only measure single-point pressure or strain and cannot obtain full-field three-dimensional stress distribution of a cartilage contact surface, and the system function stops at monitoring and does not form a monitoring-to-warning closed loop, quantifies uncertainty through a Bayesian neural network, provides a reliable basis for clinical decision-making, combines multidimensional biomechanical characteristics and an attention mechanism, accurately focuses on a stress key area, significantly improves the precision and individualization level of joint cartilage health evaluation, helps early detection and prevention of injury, classifies stress abnormalities, implements accurate early warning, and formulates an individualized treatment scheme according to uncertainty results and cartilage health indexes, and customizes a rehabilitation training plan according to user conditions, and improves the scientific nature of joint injury prevention and rehabilitation.

The invention discloses a functional composition capable of improving bone and joint health, a conditioner and application. The functional composition comprises a first active component and a second active component, the first active component is prepared from elastin peptide, haematococcus pluvialis and ergothioneine; the second active component is prepared from one or more of herba houttuyniae and non-denatured type II collagen. Through scientific compatibility of all the components, the levels of glycosaminoglycan in chondrocytes and osteocalcin in osteoblasts are synergistically improved, and the protection effect on joint, cartilage and skeleton health is achieved. Meanwhile, a plurality of components in the functional composition capable of improving the health of the bones and the joints are natural in source, good in biocompatibility and free of obvious side effects, and a medicine or an alternative scheme thereof is provided for treatment and prevention of the bone diseases.

The invention relates to the technical field of biological pharmacy, and particularly discloses targeting drug-loaded nanoparticles for treating rheumatoid arthritis (RA) as well as a preparation method and application thereof, and the technical key points are as follows: on the basis that a GLP-1R agonist (such as liraglutide) is proved to be capable of effectively relieving rheumatoid arthritis symptoms, the targeting drug-loaded nanoparticles can be used for treating rheumatoid arthritis; gLP-1RA is wrapped by the silk fibroin nanoparticles to prepare the drug-loaded nanoparticles, and the drug-loaded nanoparticles can effectively improve the stability of GLP-1RA, prolong retention time in vivo and improve the curative effect of the drug; the drug-loaded nanoparticles and the type II collagen targeting peptide (WYRGRL) are crosslinked, so that the drug-loaded nanoparticles target the articular cartilage, the drug is gathered on the articular cartilage to be attached, the local concentration of the drug and the availability of the drug are further improved, and the curative effect of inhibiting the progress of RA is further enhanced; for lesion facet joints in RA, the treatment scheme of micro-needle administration can realize slow release of drugs, and the micro-needle is convenient to use and is suitable for patients to use at home.

The application provides a kind of joint microenvironment regulating hydrogel and application, belong to medical and biomedical material technical field, raw material includes methacrylated aldehyde-based hyaluronic acid, RGDS grafted aldehyde-based hyaluronic acid, epsilon-polylysine and GW1100, the above raw materials are added into buffer solution and mixed to obtain gel precursor solution, the gel precursor solution is photocured to obtain the joint microenvironment regulating hydrogel.The raw material of the joint microenvironment regulating hydrogel is widely sourced, green and environmentally friendly, low cost, the preparation method of the hydrogel is simple, and there is no organic solvent residue.The experimental results prove that the joint microenvironment regulating hydrogel has good biocompatibility and biological activity, and has good application prospect in joint cartilage repair and tissue regeneration.

The invention relates to the technical field of cell preparations, in particular to a method for rapidly evaluating the repairing function of cartilage cells on cartilage injury at low cost, which comprises the following steps: constructing a cartilage injury model by adopting pig knee and femoral joint cartilage tissues, inoculating cell spheres formed by the to-be-evaluated cartilage cells to an injury part of the cartilage injury model, and evaluating the cartilage injury part of the cartilage injury model. And transplanting the cartilage cells into an experimental animal body, and evaluating the repair function of the cartilage cells according to the repair condition of the cartilage injury model. The method provides a systematic scheme for evaluating the repairing function of the cartilage cells, has the advantages of simplicity in operation, short time consumption, low cost, high stability and the like, and provides an effective method for evaluating the functions of the cartilage cells and screening and evaluating the functions of cells such as induced pluripotent stem cells with cartilage injury repairing potential; the method has a good application prospect in development of cell preparations for cartilage injury repair.

The utility model relates to the technical field of medical instruments, in particular to a shoulder arthroscopy thread catcher which comprises a handle, a sliding rod, a metal tube and an elastic thread, an axis hole is axially formed in a body of the handle, a groove is formed in the side face of the body, and the groove is communicated with the axis hole; the sliding rod is inserted into the axis hole in a sliding manner; the metal tube comprises a straight tube section and a bent tube section, a tube cavity of the straight tube section and a tube cavity of the bent tube section are communicated, a puncture tip is arranged at the end of the bent tube section, a wire outlet communicated with the tube cavity is formed in the side face close to the puncture tip, the metal tube is connected with the end face of the handle through the straight tube section, and the tube cavity of the straight tube section is communicated with the axis hole; one end of the elastic silk thread is close to the puncture tip and connected to the bent pipe section, and the other end of the elastic silk thread penetrates through the thread outlet and a pipe cavity of the metal pipe and then is connected with one end of the sliding rod. The utility model has the advantages of simple structure, low cost and more accurate and quicker line grasping, can reduce the frequency of replacing an observation approach, and is beneficial to shortening the operation time and avoiding articular cartilage injury.

The application discloses a kind of for repairing cartilage decellularized cartilage matrix support and apoptotic corpuscle composition and its preparation method, composition includes: microporous support, support includes decellularized cartilage outer matrix and crosslinked organic polymer;Apoptotic corpuscle, apoptotic corpuscle exists in microporous support;Wherein, based on the volume 3.14mm 3 Microporous support, 0.01-0.02ug of apoptotic corpuscle is included.The combined support of the application can improve the activity of apoptotic corpuscle, prolong the action time of apoptotic corpuscle, achieve the effect of slow release, also can regulate joint cavity microenvironment, effectively promote joint cartilage repair, improve the repair effect of loss cartilage, avoid the security risks brought by traditional exogenous cell transplantation.

The invention relates to the technical field of machine learning, in particular to a knee osteoarthritis assessment method based on machine learning, which comprises the following steps: acquiring knee joint T1 and T2 weighted images of a patient with knee osteoarthritis; manually segmenting the knee joint cartilage in the T1 weighted image and measuring the volume of the knee joint cartilage; cutting the T2 weighted image to obtain a meniscus image, manually segmenting the meniscus image to obtain annotation data, and constructing an image data set; training a meniscus automatic segmentation model, and segmenting the meniscus in the meniscus image; carrying out three-classification on meniscus pixels of the middle five layers of the meniscus image, and calculating a meniscus space specificity signal index; the performance of the meniscus space specific signal index in diagnosis of knee osteoarthritis is systematically evaluated. According to the knee joint meniscus damage diagnosis method, the knee joint T1 and T2 weighted images of a patient with knee osteoarthritis are collected, the meniscus automatic segmentation model and a meniscus space specificity signal index calculation method are applied, the damage condition of the knee joint meniscus is accurately evaluated, and knee osteoarthritis diagnosis is achieved. The process can provide a reliable diagnosis basis for orthopedists, so that clinical diagnosis of knee osteoarthritis is effectively assisted.

This invention relates to a temperature-induced self-assembly cartilage repair gel, its preparation method, and its applications, belonging to the field of biomedical materials technology. The gel is prepared by dissolving recombinant type II humanized collagen and sodium hyaluronate in a mass ratio of 0.7:1 to 7:1 in 0.01M phosphate buffer and allowing it to stand at 4±2℃ for at least 3 days to complete self-assembly. Under specific ionic strength and pH conditions, the low temperature of 4±2℃ drives the recombinant type II humanized collagen and sodium hyaluronate to form an ordered interpenetrating network structure through non-covalent supramolecular interactions, endowing the gel with excellent mechanical properties and anti-enzymatic stability, while significantly promoting the proliferation, migration, and chondrogenic differentiation of bone marrow mesenchymal stem cells. A rat cartilage defect model confirms the gel's excellent in vivo repair effect. The gel can be easily extruded through a 22G-25G injection needle and is suitable for minimally invasive injection treatment of joint cartilage defects.

The invention discloses an articular cartilage stress dynamic monitoring system based on flexible sensing and AI, and belongs to the technical field of medical health monitoring. According to the invention, the problems that only single-point pressure or strain can be measured and full-field and three-dimensional stress distribution of a cartilage contact surface cannot be obtained in the prior art are solved; according to the method, the problems that the system functions stop monitoring and a closed loop from monitoring to early warning is not formed are solved, the uncertainty is quantified through the Bayesian neural network, and a reliable basis is provided for clinical decision making; by combining multi-dimensional biomechanical characteristics and an attention mechanism, a stress key area is accurately focused, the accuracy and individuation level of articular cartilage health assessment are remarkably improved, and early discovery and prevention of injuries are assisted; meanwhile, stress abnormity is graded, accurate early warning is implemented, and a personalized treatment scheme is formulated according to an uncertainty result and cartilage health indexes; a rehabilitation training plan is customized according to user conditions, and the scientificity of joint injury prevention and rehabilitation is improved.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to dexamethasone single-phase zero-order release sustained-release microspheres as well as a preparation method and application thereof. The dexamethasone sustained-release microspheres are prepared by an emulsification-solvent evaporation method, the release platform phase of the microspheres is improved by adding a release accelerator, and finally the dexamethasone sustained-release microspheres with a single-phase zero-order release behavior are obtained. Experimental results show that various representations of the sustained-release microspheres meet the requirements of preparations, and in-vivo pharmacodynamic studies of rats also show that the sustained-release microspheres can protect articular cartilage and maintain a normal cartilage structure, and have the same curative effect as commercially available dexamethasone acetate injection. Therefore, the dexamethasone sustained-release microsphere which is used for articular cavity injection and has a good release behavior is successfully developed, the advantages of articular cavity injection drugs can be fully played, the drugs can be continuously and stably released, long-acting treatment of arthritis is achieved, and the dexamethasone sustained-release microsphere has important clinical application value and wide market prospects.

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition, a traditional Chinese medicine extracting solution and a related preparation method and application. The traditional Chinese medicine composition is prepared from folium cortex eucommiae, fructus corni, herba cistanche, radix astragali, semen coicis, radix angelicae sinensis, radix codonopsis, rhizoma polygonati, rhizoma dioscoreae and radix glycyrrhizae. Compared with the prior art, the traditional Chinese medicine composition provided by the embodiment of the invention has the advantages that the folium cortex eucommiae has the effects of tonifying liver and kidney and strengthening muscles and bones, the radix astragali has the effects of tonifying qi and relieving arthralgia, the semen coicis has the effects of eliminating dampness and relieving arthralgia, the fructus corni and the herba cistanche have the effects of tonifying kidney yang, the rhizoma polygonati and the Chinese yam have the effects of promoting kidney yin, the angelica sinensis has the effects of replenishing blood, the codonopsis pilosula has the effects of tonifying qi and the liquorice has the effects of harmonizing qi; osteoarthritis related symptoms are improved, and side effects are small.

This invention relates to a method for constructing a humanized pig model of type II collagen and its application, belonging to the field of gene modification technology. The invention first provides a CRISPR-Cas9 system for targeted editing of the porcine type II collagen α1 gene. Using CRISPR / Cas9-mediated homologous recombination, the porcine COL2A1 gene is orally replaced with the human COL2A1 gene. Then, using somatic cell cloning technology, a humanized pig model of type II collagen is constructed, allowing the pig to produce cartilage tissue more similar to that of humans, reducing post-transplant immune rejection. This is expected to fundamentally solve the problem of human cartilage tissue shortage and has significant implications for the treatment of articular cartilage damage, providing a better and more suitable transplant material for the treatment of articular cartilage damage.

The present invention relates to a biomaterial comprising: —a membrane wound patch (a), made of a nanofibrous polymeric scaffold, —a hydrogel (b) including autologous or allogenic bone marrow-derived mesenchymal stem cells, and —a bone wound patch (c) being a nanofibrous scaffold made of polymers, wherein said scaffold has a surface coated with an interrupted coating made of multilayered droplets, said multilayered droplets being droplets composed of at least one layer pair consisting of a layer of polyanions and a layer of polycations, and wherein the bone wound patch (c) further comprises a bone growth factor; wherein the hydrogel (b) is included between the membrane wound patch (a) and the bone wound patch (c).

The application discloses a functional composition, a conditioner and a use for treating or preventing bone and joint diseases. The composition of the functional composition comprises a first active component and a second active component, wherein the first active component comprises elastin peptide 1-500 mu g / mL, haematococcus pluvialis 40-20 mu g / mL, phyllanthus emblica 8-4 mu g / mL and mussel 1-500 mu g / mL in terms of concentration in the functional composition; and the second active component comprises broccoli water extract and / or boric acid substances. The application realizes the synergistic improvement of glycosaminoglycan and osteocalcin levels by scientific compounding, plays a protective role on joint, cartilage and bone health, and has natural, safe, good biocompatibility and no obvious side effects, and provides a kind of drug or a replacement scheme for bone disease treatment and prevention.

The application discloses application of a substance with increased OSKM content or activity in preventing joint cartilage aging and treating osteoarthritis diseases. The OSKM of the application is obtained by connecting Oct4, Sox2, Klf4 and C-Myc. The experiment of the application proves that the OSKM or the substance with increased OSKM content or activity has a definite effect in preventing joint cartilage aging and treating osteoarthritis diseases, the application provides a new strategy for developing gene therapy for preventing joint cartilage aging and treating osteoarthritis diseases, and expands the selectable range of clinical gene therapy and drug therapy.

The invention relates to the technical field of personal health risk assessment, and particularly provides an ultrasonic image processing method for gout articular cartilage injury grading, which comprises a double sign degree acquisition module, a first screening module, a confidence coefficient acquisition module and a second screening module, the method comprises the following steps: determining the double-track sign degree of multiple frames of initial ultrasonic images of gout articular cartilage at different angles, screening to obtain intermediate ultrasonic images, and determining the cartilage injury assessment confidence of each target ultrasonic image set when the intermediate ultrasonic image with the maximum elastic modulus mean value of the double-track sign area is used for target ultrasonic image set division each time, so as to determine the cartilage injury assessment confidence of each target ultrasonic image set. And the target ultrasonic image set corresponding to the maximum cartilage injury evaluation confidence is taken as the final ultrasonic image, so that the accuracy and reliability of ultrasonic image screening are improved, ultrasonic images with low reference value or even without reference value are prevented from participating in model training, and the reliability of a gout articular cartilage injury grading model is improved.

The utility model discloses a knee joint cartilage transplantation instrument which comprises a holding part, a bone taking sheathing canal and a rod piece connecting the holding part and the bone taking sheathing canal, the rod piece is hollow, one end of the rod piece is provided with sawteeth to form a sawtooth end, the bone taking sheathing canal is arranged on the periphery of the sawtooth end in a sleeved mode and used for abutting against cartilage, and the sawtooth end is used for cutting the cartilage. A suction pipe at least partially located in the sawtooth end is further arranged in the rod piece, the suction pipe is provided with a suction part adaptive to the inner diameter of the sawtooth end, and the suction pipe is configured to move in the extending direction of the rod piece in a drivable mode so that the suction part can move in the sawtooth end and suck air in the sawtooth end. Compared with the prior art, the knee joint cartilage transplantation instrument has the advantages that bone taking can be completed without knocking, and injury to a patient caused by knocking is avoided.

The invention belongs to the technical field of animal-derived active polypeptides, and discloses a He-prescription crucian carp skin collagen peptide with anti-osteoarthritis activity and application thereof. After being subjected to in-vitro simulated digestion, the He-prescription crucian carp skin collagen peptide comprises 10 peptide fragments which are respectively LVGPPGLT, IGMPGMT, LIGPPGL, NIGMPGM, IGPGPI, LTGFPGAA, GFNGLPGS, MTGPIGL, VGPPGAP and GLPGLAGR. The invention also discloses that the combined-prescription crucian skin collagen peptide has anti-osteoarthritis activity, can be further used for preparing anti-osteoarthritis products, can obviously relieve hyperalgesia behaviors and anxiety depression behaviors caused by osteoarthritis of mice, and can obviously improve the defects of rough surface, serious abrasion and poor anti-osteoarthritis of knee joint cartilage of mice caused by osteoarthritis. The number of cartilage cells is reduced, and joint matrix protein is lost.

The invention discloses a nutritional composition for promoting osteogenesis, protecting articular cartilage and improving joint inflammation, and belongs to the technical field of bone health products. According to the invention, the components in the formula and the proportion thereof are systematically researched and analyzed to construct a nutritional composition formula system which takes yak bone collagen peptide as a main material and shark cartilage powder as an auxiliary material, and is added with an effective amount of non-denatured type II collagen and vitamin K2 to promote osteogenesis, protect articular cartilage and improve joint inflammation. On the basis of research on bioactive substances in combination with the modern nutriology theory, the best combination between bone collagen peptide and cartilage powder, between vitamin K2 and bone collagen peptide and between non-denatured type II collagen and bone collagen is found through tests, the synergistic effect is achieved, and the bone nutrition effect is extremely good. Functional tests and testee people prove that the nutritional composition has the effects of promoting osteogenesis, protecting articular cartilage and improving joint inflammation.

The invention discloses a targeted exosome-hydrogel preparation as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The preparation is composed of an umbilical cord mesenchymal stem cell exosome which is subjected to targeting modification by cartilage affinity peptide, therapeutic nucleic acid loaded in the umbilical cord mesenchymal stem cell exosome, and PLGA-PEG-PLGA temperature-sensitive hydrogel encapsulating the carrier. The preparation method comprises the following steps: efficiently preparing an active exosome by combining three-dimensional dynamic hypoxia culture with TSG-6 agonist stimulation; rapid and mild separation is carried out by using a micro-fluidic chip; carrying out CAP peptide targeted modification through chemical coupling; and finally, mixing the drug-loaded exosome with the pH response type hydrogel precursor solution to further prepare freeze-dried powder. Through combination of targeted modification and the intelligent hydrogel carrier, the enrichment retention capacity of the exosome in the articular cartilage and the response release efficiency of the exosome in a lesion environment are remarkably improved, collaborative treatment of anti-inflammation and cartilage regeneration promotion is achieved, and the preparation is good in stability and lasting in effect.

The invention belongs to the technical field of experimental model preparation, and provides a construction method of an in-vitro articular cartilage superficial layer injury model aiming at proteoglycan and collagenous fibers in a superficial cartilage layer (SFZ) in order to simulate cartilage surface component loss and structural damage in the early stage of OA morbidity, and the in-vitro articular cartilage superficial layer injury model is constructed by taking a pig knee joint cartilage as a material. A mixed solution of 0.01 mg / ml chymotrypsin and 0.5 mg / ml collagenase D is used as digestive enzyme, the surface of an in-vitro articular cartilage column is treated for 20 min, proteoglycan and collagenous fibers in SFZ are accurately destroyed, and an in-vitro articular cartilage superficial layer damage model is constructed; histological staining, microscopic observation and mechanical detection results all show that the method can cause SFZ structure damage and mechanical property reduction on the premise of not influencing deep tissues. The model has the advantages of simplicity and convenience in operation, low cost and controllable injury range, and provides a reliable experimental tool for research on early pathological mechanisms of osteoarthritis and exploration of related diagnosis and treatment means.

The present application relates to the technical field of personal health risk assessment, and specifically provides an ultrasonic image processing method for gout joint cartilage injury grading, comprising a double-track sign degree acquisition module, a first screening module, a confidence degree acquisition module and a second screening module, the double-track sign degree of multiple frames of initial ultrasonic images of different angles of gout joint cartilage is determined, and intermediate ultrasonic images are obtained through screening, in each time, the maximum intermediate ultrasonic image is taken as the target ultrasonic image set, the cartilage injury evaluation confidence of each target ultrasonic image set is determined, the target ultrasonic image set corresponding to the maximum cartilage injury evaluation confidence is taken as the final ultrasonic image, the accuracy and reliability of ultrasonic image screening are improved, and the ultrasonic images with little reference value or even no reference value are avoided to participate in model training, and the reliability of the gout joint cartilage injury grading model is improved.

The invention discloses a functional composition for treating or preventing bone and joint diseases, a conditioner and application. The functional composition comprises a first active component and a second active component, and according to the concentration of the functional composition, the first active component comprises 1 [mu] g / mL-500 [mu] g / mL of elastin peptide, 40 ng / mL-20 [mu] g / mL of haematococcus pluvialis, 8 ng / mL-4 [mu] g / mL of emblic leafflower fruit and 1 [mu] g / mL-500 [mu] g / mL of mussel; the second active component comprises a broccoli aqueous extract and / or boric acid substances. Through scientific compatibility, all the components synergistically improve the levels of glycosamine poly and osteocalcin, the health of joints, cartilages and bones is protected, the components are natural in source, safe, good in biocompatibility and free of obvious side effects, and a medicine or an alternative scheme thereof is provided for treatment and prevention of bone diseases.

The device comprises a protection pad, a stabilizing assembly is arranged on the protection pad, a pair of rotating shafts are arranged on one side of the protection pad through fixing columns, sleeves are rotationally clamped to the outer sides of the two rotating shafts, the two sleeves are connected through a rotating frame, and the stabilizing assembly comprises connecting bases arranged at the two ends of the rotating frame; a clamping wheel is rotationally connected to the connecting seat, traction ropes are clamped on the outer side of the clamping wheel, a winder is arranged on the rear side of the protection pad, the winder is connected with one ends of the two traction ropes, and the free ends of the two traction ropes are connected through an inflation ring; the stabilizing assembly arranged in the knee joint cartilage removing device can press the knee joint cartilage of a patient, when an operator removes the seriously damaged cartilage of the patient, the stabilizing assembly can press and fix other cartilage which does not need to be removed, and therefore displacement or falling of other cartilage is reduced, and after the pressing film presses the cartilage on one side of the knee joint of the patient, the cartilage can be removed. And the pressed film can be cut open according to the previously confirmed position to remove part of seriously worn articular cartilage.

The application belongs to the technical field of polymer materials, and particularly relates to a three-layer gradient self-healing hydrogel as well as a preparation method and application thereof. The application provides a composition for preparing the three-layer gradient self-healing hydrogel, which comprises composition A, composition B and / or composition C; the composition A and the composition B are both composed of modified chondroitin sulfate, carboxymethyl chitosan and tannic acid, and the difference lies in that the tannic acid concentration in the composition A is 0.5-1.5% w / v, and the tannic acid concentration in the composition B is 2.5-3.5% w / v; the composition C comprises modified chondroitin sulfate, carboxymethyl chitosan, tannic acid and nano-hydroxyapatite, and the tannic acid concentration is 2.5-3.5% w / v. The three-layer gradient self-healing hydrogel has good self-healing performance, mechanical property, tissue adhesion performance and antioxidant property, and can be applied to integrated repair of joint cartilage-bone tissue.