34 results about "Joint cartilage" patented technology

A drill-less method of stabilizing a sacroiliac joint using a multimodal abrading device to create a rich environment for bony fusion. The unique decortication instrument includes an integral slap hammer assembly joined with a rasp-box osteotome tooling head to simultaneously remove cartilage from the joint while preparing the sacrum and ilium down to bleeding bone. Subsequent to joint preparation, an implant with a large bone graft window may be implanted into the prepared SI joint through a specially designed access cannula.

The application discloses a functional composition for improving bone and joint health, a conditioner and application. The functional composition comprises a first active component and a second active component; the first active component comprises elastin peptide, haematococcus pluvialis and ergothioneine; and the second active component comprises one or more of houttuynia cordata and non-denatured type II collagen. The application realizes the protection effect on joint, cartilage and bone health by scientific matching of the components and synergistically improving the glycosaminoglycan level in chondrocytes and the osteocalcin level in osteoblasts. Meanwhile, the components in the functional composition for improving bone and joint health are natural and have good biocompatibility, and have no obvious side effects, so that the application provides a kind of drug or a substitute for the treatment and prevention of bone diseases.

The application discloses a joint cartilage stress dynamic monitoring system based on flexible sensing and AI and belongs to the technical field of medical health monitoring.The application solves the problem that the prior art can only measure single-point pressure or strain and cannot obtain full-field three-dimensional stress distribution of a cartilage contact surface, and the system function stops at monitoring and does not form a monitoring-to-warning closed loop, quantifies uncertainty through a Bayesian neural network, provides a reliable basis for clinical decision-making, combines multidimensional biomechanical characteristics and an attention mechanism, accurately focuses on a stress key area, significantly improves the precision and individualization level of joint cartilage health evaluation, helps early detection and prevention of injury, classifies stress abnormalities, implements accurate early warning, and formulates an individualized treatment scheme according to uncertainty results and cartilage health indexes, and customizes a rehabilitation training plan according to user conditions, and improves the scientific nature of joint injury prevention and rehabilitation.

The invention discloses a functional composition capable of improving bone and joint health, a conditioner and application. The functional composition comprises a first active component and a second active component, the first active component is prepared from elastin peptide, haematococcus pluvialis and ergothioneine; the second active component is prepared from one or more of herba houttuyniae and non-denatured type II collagen. Through scientific compatibility of all the components, the levels of glycosaminoglycan in chondrocytes and osteocalcin in osteoblasts are synergistically improved, and the protection effect on joint, cartilage and skeleton health is achieved. Meanwhile, a plurality of components in the functional composition capable of improving the health of the bones and the joints are natural in source, good in biocompatibility and free of obvious side effects, and a medicine or an alternative scheme thereof is provided for treatment and prevention of the bone diseases.

This invention relates to a temperature-induced self-assembly cartilage repair gel, its preparation method, and its applications, belonging to the field of biomedical materials technology. The gel is prepared by dissolving recombinant type II humanized collagen and sodium hyaluronate in a mass ratio of 0.7:1 to 7:1 in 0.01M phosphate buffer and allowing it to stand at 4±2℃ for at least 3 days to complete self-assembly. Under specific ionic strength and pH conditions, the low temperature of 4±2℃ drives the recombinant type II humanized collagen and sodium hyaluronate to form an ordered interpenetrating network structure through non-covalent supramolecular interactions, endowing the gel with excellent mechanical properties and anti-enzymatic stability, while significantly promoting the proliferation, migration, and chondrogenic differentiation of bone marrow mesenchymal stem cells. A rat cartilage defect model confirms the gel's excellent in vivo repair effect. The gel can be easily extruded through a 22G-25G injection needle and is suitable for minimally invasive injection treatment of joint cartilage defects.

The invention discloses an articular cartilage stress dynamic monitoring system based on flexible sensing and AI, and belongs to the technical field of medical health monitoring. According to the invention, the problems that only single-point pressure or strain can be measured and full-field and three-dimensional stress distribution of a cartilage contact surface cannot be obtained in the prior art are solved; according to the method, the problems that the system functions stop monitoring and a closed loop from monitoring to early warning is not formed are solved, the uncertainty is quantified through the Bayesian neural network, and a reliable basis is provided for clinical decision making; by combining multi-dimensional biomechanical characteristics and an attention mechanism, a stress key area is accurately focused, the accuracy and individuation level of articular cartilage health assessment are remarkably improved, and early discovery and prevention of injuries are assisted; meanwhile, stress abnormity is graded, accurate early warning is implemented, and a personalized treatment scheme is formulated according to an uncertainty result and cartilage health indexes; a rehabilitation training plan is customized according to user conditions, and the scientificity of joint injury prevention and rehabilitation is improved.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to dexamethasone single-phase zero-order release sustained-release microspheres as well as a preparation method and application thereof. The dexamethasone sustained-release microspheres are prepared by an emulsification-solvent evaporation method, the release platform phase of the microspheres is improved by adding a release accelerator, and finally the dexamethasone sustained-release microspheres with a single-phase zero-order release behavior are obtained. Experimental results show that various representations of the sustained-release microspheres meet the requirements of preparations, and in-vivo pharmacodynamic studies of rats also show that the sustained-release microspheres can protect articular cartilage and maintain a normal cartilage structure, and have the same curative effect as commercially available dexamethasone acetate injection. Therefore, the dexamethasone sustained-release microsphere which is used for articular cavity injection and has a good release behavior is successfully developed, the advantages of articular cavity injection drugs can be fully played, the drugs can be continuously and stably released, long-acting treatment of arthritis is achieved, and the dexamethasone sustained-release microsphere has important clinical application value and wide market prospects.

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition, a traditional Chinese medicine extracting solution and a related preparation method and application. The traditional Chinese medicine composition is prepared from folium cortex eucommiae, fructus corni, herba cistanche, radix astragali, semen coicis, radix angelicae sinensis, radix codonopsis, rhizoma polygonati, rhizoma dioscoreae and radix glycyrrhizae. Compared with the prior art, the traditional Chinese medicine composition provided by the embodiment of the invention has the advantages that the folium cortex eucommiae has the effects of tonifying liver and kidney and strengthening muscles and bones, the radix astragali has the effects of tonifying qi and relieving arthralgia, the semen coicis has the effects of eliminating dampness and relieving arthralgia, the fructus corni and the herba cistanche have the effects of tonifying kidney yang, the rhizoma polygonati and the Chinese yam have the effects of promoting kidney yin, the angelica sinensis has the effects of replenishing blood, the codonopsis pilosula has the effects of tonifying qi and the liquorice has the effects of harmonizing qi; osteoarthritis related symptoms are improved, and side effects are small.

The present invention relates to a biomaterial comprising: —a membrane wound patch (a), made of a nanofibrous polymeric scaffold, —a hydrogel (b) including autologous or allogenic bone marrow-derived mesenchymal stem cells, and —a bone wound patch (c) being a nanofibrous scaffold made of polymers, wherein said scaffold has a surface coated with an interrupted coating made of multilayered droplets, said multilayered droplets being droplets composed of at least one layer pair consisting of a layer of polyanions and a layer of polycations, and wherein the bone wound patch (c) further comprises a bone growth factor; wherein the hydrogel (b) is included between the membrane wound patch (a) and the bone wound patch (c).

The application discloses a functional composition, a conditioner and a use for treating or preventing bone and joint diseases. The composition of the functional composition comprises a first active component and a second active component, wherein the first active component comprises elastin peptide 1-500 mu g / mL, haematococcus pluvialis 40-20 mu g / mL, phyllanthus emblica 8-4 mu g / mL and mussel 1-500 mu g / mL in terms of concentration in the functional composition; and the second active component comprises broccoli water extract and / or boric acid substances. The application realizes the synergistic improvement of glycosaminoglycan and osteocalcin levels by scientific compounding, plays a protective role on joint, cartilage and bone health, and has natural, safe, good biocompatibility and no obvious side effects, and provides a kind of drug or a replacement scheme for bone disease treatment and prevention.

The application discloses application of a substance with increased OSKM content or activity in preventing joint cartilage aging and treating osteoarthritis diseases. The OSKM of the application is obtained by connecting Oct4, Sox2, Klf4 and C-Myc. The experiment of the application proves that the OSKM or the substance with increased OSKM content or activity has a definite effect in preventing joint cartilage aging and treating osteoarthritis diseases, the application provides a new strategy for developing gene therapy for preventing joint cartilage aging and treating osteoarthritis diseases, and expands the selectable range of clinical gene therapy and drug therapy.

The utility model discloses a knee joint cartilage transplantation instrument which comprises a holding part, a bone taking sheathing canal and a rod piece connecting the holding part and the bone taking sheathing canal, the rod piece is hollow, one end of the rod piece is provided with sawteeth to form a sawtooth end, the bone taking sheathing canal is arranged on the periphery of the sawtooth end in a sleeved mode and used for abutting against cartilage, and the sawtooth end is used for cutting the cartilage. A suction pipe at least partially located in the sawtooth end is further arranged in the rod piece, the suction pipe is provided with a suction part adaptive to the inner diameter of the sawtooth end, and the suction pipe is configured to move in the extending direction of the rod piece in a drivable mode so that the suction part can move in the sawtooth end and suck air in the sawtooth end. Compared with the prior art, the knee joint cartilage transplantation instrument has the advantages that bone taking can be completed without knocking, and injury to a patient caused by knocking is avoided.

The invention belongs to the technical field of animal-derived active polypeptides, and discloses a He-prescription crucian carp skin collagen peptide with anti-osteoarthritis activity and application thereof. After being subjected to in-vitro simulated digestion, the He-prescription crucian carp skin collagen peptide comprises 10 peptide fragments which are respectively LVGPPGLT, IGMPGMT, LIGPPGL, NIGMPGM, IGPGPI, LTGFPGAA, GFNGLPGS, MTGPIGL, VGPPGAP and GLPGLAGR. The invention also discloses that the combined-prescription crucian skin collagen peptide has anti-osteoarthritis activity, can be further used for preparing anti-osteoarthritis products, can obviously relieve hyperalgesia behaviors and anxiety depression behaviors caused by osteoarthritis of mice, and can obviously improve the defects of rough surface, serious abrasion and poor anti-osteoarthritis of knee joint cartilage of mice caused by osteoarthritis. The number of cartilage cells is reduced, and joint matrix protein is lost.

The invention discloses a nutritional composition for promoting osteogenesis, protecting articular cartilage and improving joint inflammation, and belongs to the technical field of bone health products. According to the invention, the components in the formula and the proportion thereof are systematically researched and analyzed to construct a nutritional composition formula system which takes yak bone collagen peptide as a main material and shark cartilage powder as an auxiliary material, and is added with an effective amount of non-denatured type II collagen and vitamin K2 to promote osteogenesis, protect articular cartilage and improve joint inflammation. On the basis of research on bioactive substances in combination with the modern nutriology theory, the best combination between bone collagen peptide and cartilage powder, between vitamin K2 and bone collagen peptide and between non-denatured type II collagen and bone collagen is found through tests, the synergistic effect is achieved, and the bone nutrition effect is extremely good. Functional tests and testee people prove that the nutritional composition has the effects of promoting osteogenesis, protecting articular cartilage and improving joint inflammation.

The invention belongs to the technical field of experimental model preparation, and provides a construction method of an in-vitro articular cartilage superficial layer injury model aiming at proteoglycan and collagenous fibers in a superficial cartilage layer (SFZ) in order to simulate cartilage surface component loss and structural damage in the early stage of OA morbidity, and the in-vitro articular cartilage superficial layer injury model is constructed by taking a pig knee joint cartilage as a material. A mixed solution of 0.01 mg / ml chymotrypsin and 0.5 mg / ml collagenase D is used as digestive enzyme, the surface of an in-vitro articular cartilage column is treated for 20 min, proteoglycan and collagenous fibers in SFZ are accurately destroyed, and an in-vitro articular cartilage superficial layer damage model is constructed; histological staining, microscopic observation and mechanical detection results all show that the method can cause SFZ structure damage and mechanical property reduction on the premise of not influencing deep tissues. The model has the advantages of simplicity and convenience in operation, low cost and controllable injury range, and provides a reliable experimental tool for research on early pathological mechanisms of osteoarthritis and exploration of related diagnosis and treatment means.

The present application relates to the technical field of personal health risk assessment, and specifically provides an ultrasonic image processing method for gout joint cartilage injury grading, comprising a double-track sign degree acquisition module, a first screening module, a confidence degree acquisition module and a second screening module, the double-track sign degree of multiple frames of initial ultrasonic images of different angles of gout joint cartilage is determined, and intermediate ultrasonic images are obtained through screening, in each time, the maximum intermediate ultrasonic image is taken as the target ultrasonic image set, the cartilage injury evaluation confidence of each target ultrasonic image set is determined, the target ultrasonic image set corresponding to the maximum cartilage injury evaluation confidence is taken as the final ultrasonic image, the accuracy and reliability of ultrasonic image screening are improved, and the ultrasonic images with little reference value or even no reference value are avoided to participate in model training, and the reliability of the gout joint cartilage injury grading model is improved.

The invention discloses a functional composition for treating or preventing bone and joint diseases, a conditioner and application. The functional composition comprises a first active component and a second active component, and according to the concentration of the functional composition, the first active component comprises 1 [mu] g / mL-500 [mu] g / mL of elastin peptide, 40 ng / mL-20 [mu] g / mL of haematococcus pluvialis, 8 ng / mL-4 [mu] g / mL of emblic leafflower fruit and 1 [mu] g / mL-500 [mu] g / mL of mussel; the second active component comprises a broccoli aqueous extract and / or boric acid substances. Through scientific compatibility, all the components synergistically improve the levels of glycosamine poly and osteocalcin, the health of joints, cartilages and bones is protected, the components are natural in source, safe, good in biocompatibility and free of obvious side effects, and a medicine or an alternative scheme thereof is provided for treatment and prevention of bone diseases.

The application provides a Chinese medicine composition containing Notopterygium incisum and Lonicera japonica in preparation and treatment of rheumatoid arthritis, and belongs to the technical field of medicine; scientific research shows that the Chinese medicine composition containing Notopterygium incisum and Lonicera japonica can reduce joint cartilage destruction, subchondral bone destruction caused by rheumatoid arthritis, promote osteoblast differentiation and mineralization, reduce joint swelling, synovial hyperplasia, and inflammatory cell infiltration of joints caused by rheumatoid arthritis, has the effect of treating rheumatoid arthritis, and can be developed into a medicine for treating rheumatoid arthritis.

The invention provides a ginkgo fruit peptide-containing aqueous extract for improving bone and joint health and application thereof.The preparation method of the ginkgo fruit peptide-containing aqueous extract comprises the following steps that drinking water is added into cured ginkgo fruit powder for size mixing, any two or more of amylase, alkaline protease, neutral protease and cellulase are added for step-by-step enzymolysis, and the ginkgo fruit peptide-containing aqueous extract is obtained; and after the enzymolysis is finished, carrying out solid-liquid separation, concentrating the filtrate, and carrying out spray drying to obtain the ginkgo fruit peptide-containing aqueous extract. According to the ginkgo fruit peptide-containing aqueous extract prepared by a specific enzymolysis technology, the polysaccharide content and the peptide content are remarkably increased, and the ginkgo fruit peptide-containing aqueous extract has the functional characteristic of improving bone joint health. The preparation process provided by the invention has the advantages of stability and mild conditions, can effectively improve the extraction efficiency and bioavailability of active ingredients, and provides a high-quality raw material basis for developing natural foods, health products or medicines with functions of protecting articular cartilage and relieving inflammation and bone joint related discomfort.

This invention belongs to the biomedical field, specifically relating to the use of BSP-MA / HA-NB hydrogel in the preparation of drugs for treating bone defects, cartilage damage, or degenerative diseases of articular cartilage. This invention introduces HA-NB (hyaluronic acid modified with o-nitrosobenzaldehyde) into BSP-MA (methacrylated Bletilla striata polysaccharide) to prepare BSP-MA / HA-NB hydrogel, which exhibits a significant synergistic effect in promoting cartilage and subchondral bone regeneration. Simultaneously, it can reduce inflammatory responses and promote tissue repair by inhibiting the activation of M1 macrophages and promoting the increase of M2 macrophages. It can be used to treat cartilage damage and / or degenerative diseases of articular cartilage, and has broad application prospects.

The invention provides a precise layering sterile sampling device for articular cartilage tissues. The precise layering sterile sampling device is characterized by comprising an equipment base, a mounting base, a measuring unit, a first retracting unit and a second propelling unit, the device disclosed by the invention can be used for carrying out micron-sized precise stratified sampling on articular cartilage tissues under a sterile operation condition. According to the equipment, the effect of accurately sampling layered tissues of fresh active articular cartilage is achieved, and a foundation is laid for deep research on the layered tissue structure, mechanics and layered cells of the articular cartilage.

The application belongs to the technical field of animal-derived active polypeptides, and discloses a combined crucian carp skin collagen peptide with anti-osteoarthritis activity and application thereof. After in vitro simulation digestion, the combined crucian carp skin collagen peptide contains 10 peptide segments, which are LVGPPGLT, IGMPGMT, LIGPPGL, NIGMPGM, IGPGPI, LTGFPGAA, GFNGLPGS, MTGPIGL, VGPPGAP and GLPGLAGR. The combined crucian carp skin collagen peptide has anti-osteoarthritis activity, can be further used for preparing anti-osteoarthritis products, can significantly relieve the hyperalgesia behavior and anxiety and depression-like behavior of mice caused by osteoarthritis, and can significantly improve the rough surface of knee joint cartilage, serious wear, reduction of chondrocyte number and loss of joint matrix protein of mice caused by osteoarthritis.

The application relates to the field of geriatric treatment, in particular to application of a matrix metalloproteinase inhibitor, MSAB or a pharmaceutically acceptable salt thereof, in preparation of a medicine for preventing or treating osteoarthritis cartilage injury. The application adopts the MSAB to inhibit expression of matrix metalloproteinase in chondrocytes, reduces destruction of joint cartilage structure, and achieves the effect of preventing or treating osteoarthritis.

The invention discloses composite bio-ink for 3D printing of a cartilage scaffold as well as a preparation method and application of the composite bio-ink. The composite bio-ink comprises 25-30 wt% of a biopolymer material, 20-40 wt% of a methacrylic acellular matrix, 5-15 wt% of polyethylene oxide, 0.05-0.2 wt% of a photoinitiator and ultrapure water. The composite bio-ink is used for printing a bionic cartilage repair scaffold by an electrostatic direct-writing printing technology, and the bionic cartilage repair scaffold is used for articular cartilage defect repair. The problems that an acellular matrix material is poor in mechanical property and difficult to form are solved through methacrylic modification, so that the acellular matrix material is suitable for an electrostatic direct-writing 3D printing technology, micron-scale printing precision and high porosity are achieved when a cartilage repair scaffold is manufactured, the microstructure of natural cartilage can be better simulated, and the cartilage repair scaffold has good application prospects. A better growth environment is provided for cells, and articular cartilage defect repair is promoted.

The invention provides a method for establishing a rabbit knee joint cartilage injury model in a plateau cold environment. The method comprises the following steps of: making an operative incision by taking a knee joint as a center, separating subcutaneous fascia, cutting a gap between the inner side of a patella and quadriceps femoris muscle, turning the patella outwards, and exposing a knee joint cavity and an articular surface at the far end of a femur; pore-forming is carried out on the articular surface of the distal femur until fresh blood exudation can be seen under the cartilage; and returning the patella and suturing the wound to obtain the rabbit knee joint cartilage injury model in the plateau cold environment. The model disclosed by the invention can be used for better researching a repairing mechanism of the knee joint cartilage injury in the plateau cold environment and evaluating and screening medicines and materials for treating the knee joint cartilage injury.

The application discloses a method for semi-quantitative analysis of expression level of secreted protein of human joint cartilage cells by using immunoblotting technology. The method comprises the following steps: 1) preparing a secreted protein culture solution; 2) collecting a sample of the secreted protein culture solution; 3) performing dialysis desalting treatment on the sample; 4) concentrating the secreted protein culture solution to prepare a powder; 5) re-dissolving the secreted protein culture solution powder and quantifying the protein; 6) separating the protein components in the re-dissolved culture solution; and 7) detecting the expression level of the secreted protein in the re-dissolved culture solution sample by using immunoblotting method. The low-temperature dialysis and vacuum concentration technology can significantly improve the concentration of the secreted protein in the human joint cartilage tissue culture solution sample under the condition of maintaining the integrity of the protein structure, and is suitable for detecting the chemical fluorescence signal of the secreted protein in the sample by using the immunoblotting technology. The application provides an important cell reaction detection tool for studying the pathogenesis of bone and joint diseases caused by degenerative changes of cartilage tissue and researching and developing biological small-molecule drugs for treating the diseases.

The utility model belongs to the technical field of joint support, especially a kind of fixed support for joint cartilage repair.The technology includes the first fixed plate of vertical arrangement, the second fixed plate of left and right swing is hinged in the lower side of first fixed plate, and the fixed assembly of the fixed plate is fixed in the leg of patient is arranged in the right side of first fixed plate and second fixed plate, and the support plate is vertically fixed on the front and back side wall of second fixed plate, and the notch is formed in the upper end of support plate, and the fixed column is arranged in the notch, and the fixed column is fixed on the side wall of first fixed plate, and the protection assembly for protecting the joint of patient is arranged between two fixed plates.In the utility model, under the action of fixed assembly, two fixed plates are fixed on the leg of patient on both sides of joint respectively, the joint of patient is protected by protection assembly located at joint, under the action of support plate, the burden of joint is reduced, which is helpful for the recovery of joint.

The present application relates to the field of biological medicine, and particularly relates to a collagen peptide composition for repairing joints and a preparation method thereof, mainly comprising the following raw materials: 20-25 parts of high-dispersion collagen peptide, 4-6 parts of ganoderma extract, 4-6 parts of lentinus edodes extract, 4-6 parts of composite embedded nano calcium, 2-4 parts of hyaluronic acid, 0.4-0.6 parts of vitamin K2, 0.2-0.4 parts of tea polyphenol, and 5-7 parts of pitaya fermentation original pulp; the high-dispersion collagen peptide is prepared through specific enzymolysis and ultrafiltration process, has a small molecular weight and good water solubility, and can promote joint cartilage repair and regeneration; the composite embedded nano calcium is designed through a double embedding structure, improves the bioavailability of calcium, promotes bone density increase and bone mineralization; the composition can efficiently repair joint cartilage, improve bone density and bone calcium content through synergistic action of multiple raw materials, has excellent antioxidant property, and is suitable for joint repair and bone health maintenance.

The invention discloses a highly crosslinked recombinant collagen cartilage repair scaffold material and a preparation method thereof. The material is prepared by taking recombinant II-type humanized collagen and glycosaminoglycan as raw materials through a gradient multi-stage cross-linking process of physical cross-linking, chemical cross-linking and enzyme cross-linking, and a double-layer porous scaffold with a bionic structure is formed. Wherein the upper-layer pores are in a flat shape and have the aperture of 100-200 microns, the lower-layer pores are in a vertical column shape and have the aperture of 50-100 microns, and the overall porosity is larger than 80%. Recombinant collagen is obtained by adopting a pichia pastoris eukaryotic expression system, chemical crosslinking is performed in a non-aqueous-phase isopropanol system, and the crosslinking degree, the compression modulus, the water-retaining property and the degradation resistance of the material are remarkably improved by combining enzyme crosslinking catalyzed by transglutaminase. The scaffold material has good mechanical strength and cell compatibility, can promote cartilage cell proliferation and differentiation of mesenchymal stem cells to cartilage cells, and is suitable for regeneration and repair of articular cartilage injury.

The invention provides a temporomandibular articular cartilage cell senescence model as well as a construction method and a detection method thereof, and belongs to the technical field of cell models. The invention provides a construction method of a temporomandibular articular cartilage senescence model. A low-dose topoisomerase II inhibitor is used as an inducer to induce temporomandibular articular cartilage cell senescence. By utilizing the method disclosed by the invention, typical senescence phenotypes can be obtained through induction only in 48-96 hours, the model construction period is short, and the efficiency is high; the induced cell model is more in line with the pathological process of chronic low-grade inflammation of temporomandibular joint disorder (TMD), and the phenotype is typical and stable; and the model difference between batches is small, the repeatability is good, and the cartilage characteristics are retained. The invention also improves the detection method of the temporomandibular articular cartilage cell senescence model, can directly judge whether the cell senescence is successfully induced or not through observation after the modeling is finished, has the characteristics of intuition and convenience, and is suitable for related experiments such as subsequent drug screening.