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Growth-factor-chitosan-microsphere loaded DBM support joint cartilage repairing material

A technology of chitosan microspheres and growth factors, applied in medical science, prostheses, etc., can solve the problems of high price and achieve the effects of convenient application, large internal surface area and space, and simple preparation methods

Inactive Publication Date: 2014-09-24
FIRST AFFILIATED HOSPITAL OF KUNMING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have shown that cytokines are quickly diluted and metabolized when used locally, so they need to be used repeatedly in large doses, and they are very expensive. Therefore, establishing a complete slow-release system can make growth factors more effectively act on target cells and strengthen cartilage. engineering organization

Method used

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  • Growth-factor-chitosan-microsphere loaded DBM support joint cartilage repairing material
  • Growth-factor-chitosan-microsphere loaded DBM support joint cartilage repairing material
  • Growth-factor-chitosan-microsphere loaded DBM support joint cartilage repairing material

Examples

Experimental program
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Effect test

experiment example 1

[0065] 1. Preparation of DBM

[0066] (1) Use fresh pig scapula, remove the periosteum, cartilage and soft tissue, take the cancellous bone, prepare it into a round cake shape with a diameter of 7.0 mm and a thickness of 3.0 mm, repeatedly wash with running water to remove bone marrow, blood stains and surface oil, and rinse with tap water Afterwards, store at -80°C for 3 days, in sequence;

[0067] (2) Dehydrate with absolute ethanol for 2 hours and air dry;

[0068] (3) Chloroform / methanol (1:1, volume fraction) defatted for 4 hours, air-dried;

[0069] (4) Decalcify with 0.6mol / L hydrochloric acid for 6 hours respectively (hydrochloric acid / cancellous bone: 20ml / g), and air-dry;

[0070] (5) Dehydrate with absolute ethanol for 2 hours and air dry;

[0071] (6) Chloroform / methanol (1:1, volume fraction) defatted for 4 hours, air-dried;

[0072] (7) Soak in 10% PBS (pH=7.4) at 37°C for 3 days, change the solution twice in the middle, and air dry;

[0073] (8) Disinfect w...

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Abstract

The invention discloses a growth-factor-chitosan-microsphere loaded DBM support joint cartilage repairing material. A method for preparing the growth-factor-chitosan-microsphere loaded DBM support joint cartilage repairing material includes the following steps: (1) preparing a DBM: adopting a fresh pig shoulder blade, removing the periosteum, the cartilage and the soft tissues, taking the cancellous bone of the shoulder blade, preparing the cancellous bone to be in a cake shape, carrying out repeated stirring and washing with flowing water to remove the marrow, the bloodiness and the surface grease, and after washing is carried out through the tap water, placing the cancellous bone into an environment at -80 DEG C for storage for three days to carry out proper processing; (2) preparing chitosan sustained release microspheres; (3) preparing wrapping growth factor microspheres, and calculating the encapsulation efficiency and the drug loading capacity; (4) preparing a growth-factor-chitosan-sustained-release-microsphere loaded DBM composite, and measuring amido bond connection after cross bonding is carried out a DBM support and the microspheres through EDC with the infrared ray method. The growth-factor-chitosan-microsphere loaded DBM support joint cartilage repairing material has the good cellular and biology compatibility and the good degradability, and is a good cartilage tissue engineering support material.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, and relates to a DBM scaffold loaded with growth factor chitosan microspheres used for repairing articular cartilage damage and articular cartilage repairing material. Background technique [0002] Articular cartilage damage caused by trauma or other reasons is very common in clinical practice, and cartilage itself is difficult to regenerate and repair. When articular cartilage is damaged, due to the lack of direct blood supply, lymphatic circulation and innervation of articular cartilage, mature chondrocytes cannot regenerate after injury, their self-repair ability is very limited, and the repair process after articular cartilage defect is extremely slow, regeneration There are obvious differences between tissue and normal tissue in terms of structure and function, articular cartilage degeneration is often unavoidable, and there is no completely effective treatment so far. How to rep...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/56A61L27/54
Inventor 李彦林韩睿王慧建王国梁何川王鑫
Owner FIRST AFFILIATED HOSPITAL OF KUNMING MEDICAL UNIV
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