4 results about "Cartilage tissue engineering" patented technology

The invention relates to the technical field of bioengineering, in particular to a preparation method and application of a folium cortex eucommiae fermentation extract for promoting cartilage growth. According to the method, a four-stage dynamic regulation and control process of low-temperature plasma synergistic enzymolysis pretreatment, aerobic wall breaking, aerobic and anaerobic transition and anaerobic conversion is adopted; four specific strains with complementary functions, namely bacillus subtilis, aspergillus niger, saccharomyces cerevisiae and enterococcus faecalis, are selected, and precise temperature control and pH regulation are combined, so that efficient release and conversion of active ingredients of the folium cortex eucommiae are realized. The active ingredients of the extract are rich in variety and sufficient in content, cartilage cell proliferation and osteogenic differentiation can be remarkably promoted, and the extract has full-scene cartilage repair adaptability and is good in safety through toxicology verification. The preparation process is stable and repeatable, reaches the pilot test effect, can be adapted to various dosage forms such as oral preparations, injections and the like, and is suitable for preparing cartilage repair related medicines, functional foods and cartilage tissue engineering scaffold composite materials.

The invention belongs to the technical field of cartilage tissue engineering, and discloses a cartilage tissue engineering scaffold with a cross-scale fiber structure and a preparation method and application thereof. According to the method, a handheld electrospinning gun is used, and a coarse fiber grid layer and a non-woven fiber structure layer are sequentially formed on a defective cartilage model by controlling the distance between a gun head and the cartilage defect model; and soaking in a hydrogel solution, and curing to obtain the cartilage tissue engineering scaffold. According to the method provided by the invention, the cross-scale fiber structure scaffold can be rapidly prepared on the curved surface structure, and the scaffold can be matched with the articular cartilage defect boundary and curved surface characteristics; and moreover, the scaffold can be endowed with finer and smaller regular aperture, is beneficial to colonization of stem cells, and can be widely applied to repair of cartilage defects.

The invention discloses a method for preparing active oxygen scavenging type diabetes osteochondral repair hydrogel based on a hydrothermal method and application of the active oxygen scavenging type diabetes osteochondral repair hydrogel, and belongs to the technical field of crossing of biomedical materials and tissue engineering. The method disclosed by the invention comprises the following steps: synthesizing V2CuS4 nano-enzyme with catalase-like activity through a hydrothermal method, and preparing injectable three-dimensional network hydrogel with a photoinduced gelation characteristic. Active oxygen is efficiently removed through cascade catalysis of glucose oxidase and V2CuS4 nano-enzyme, meanwhile, the methacrylamidated silk fibroin hydrogel can adapt to osteochondral defects with area difference due to the photoinduced gelation characteristic, and the individual adaptability is excellent. Under a simulated physiological environment, the prepared hydrogel is relatively high in hydroxyl radical scavenging rate and relatively good in biocompatibility, the oxidative stress regulation and control capability of an osteochondral defect repair microenvironment can be remarkably improved, and a novel functional material with mechanical support and biological activity is provided for osteochondral tissue engineering.

This invention relates to a method for quantitatively detecting residual DNA and glycosaminoglycan (GAG) levels in cartilage tissue-engineered samples. The method involves enzymatically digesting decellularized cartilage matrix samples using a combination enzyme, wherein the combination enzyme consists of papain and bromelain, with a mass ratio of papain to bromelain of 1-3:1. The digested sample is then centrifuged and ultrafiltered to obtain a test liquid containing DNA and GAG. DNA and GAG detection are then performed on the test liquid containing DNA and GAG, respectively. This invention, through the selection of enzyme types, allows the digested sample to be used for both DNA and GAG detection, improving detection efficiency and reducing the amount of tissue-engineered sample required, thus lowering sample usage costs.