125 results about "Cartilage tissue engineering" patented technology

Flexible, bioactive glass meshes and scaffolds made therefrom are provided. The meshes comprise interwoven bioactive glass fibers that can be coated with resorbable polymers. Meshes can also be woven from glass fibers and resorbable polymers. Scaffolds can be constructed by a plurality of meshes, which can have varying porosities to create porosity gradients in the scaffold. Methods of making scaffolds are provided which can comprise pulling bioactive glass fibers, winding the fibers, forming the fibers into bundles, coating the fibers with a resorbable polymer, and creating a biaxial weave with the bundles. Soft tissue engineering methods are also provided for creating scaffolds for incubating cells such as fibroblasts and chondroblasts. Meshes and scaffolds are suitable for tissue engineering, such as bone tissue engineering and cartilage tissue engineering.

A composition of porous material and gel for preparing artificial bone is prepared from collagen and porous material chosen from alpha-tricalcium phosphate/hydroxy apatite, beta-tricalcium phosphate/hydroxy apatite-coral, calcium phosphate/hydroxy apatite, calcium carbonatel tricalcium phosphate, etc.

The invention relates to a preparation method for a nanofiber porous scaffold having compression elasticity in a wet state. The preparation method comprises the following steps: dissolving gelatin and polylactic acid in a solvent so as to obtain a mixed solution, and carrying out electrostatic spinning so as to obtain a nanofiber membrane; feeding the nanofiber membrane into tertiary butanol, smashing, freezing and freeze-drying so as to obtain a non-crosslinked freeze-dried scaffold; immersing the non-crosslinked freeze-dried scaffold into cross-linking liquid, washing, soaking in deionized water, freezing and freeze-drying so as to obtain the nanofiber porous scaffold. The nanofiber porous scaffold prepared through the preparation method has degradability, biocompatibility and relatively high porosity, and has a certain compression resistance at the wet state, can quickly stop bleeding, is beneficial to cell adhesion, cell proliferation and tissue regeneration, and can be applicable to hemostatic materials, cartilage tissue engineering, skin tissue engineering and other fields.

The invention discloses a cartilage tissue engineering repair bracket and a preparation method thereof, and belongs to the cartilage tissue repair field. The bracket comprises natural high polymer water-gel and decalcified bone matrix which is coupled with affinitive peptides of mesenchymal stem cells on surface; the affinitive peptides of the mesenchymal stem cells are coupled on the surface of the decalcified bone matrix to obtain the cartilage tissue engineering repair bracket with special mesenchymal stem cell affinity. By being placed in a cartilage defective region, the cartilage tissue engineering repair bracket can collect a plurality of BMSC (bone marrow stromal cells); and in the bracket, under mating effect of the natural high polymer water-gel and the decalcified bone matrix, the BMSC can be retained in the cartilage defective region for a long time, so that the cartilage repair has a longer curative effect period. The invention further discloses a preparation method of the bracket, wherein the natural high polymer water-gel aqueous liquor is injected in the decalcified bone matrix which is coupled with affinitive peptides of mesenchymal stem cells on surface to carry out gelation reaction to obtain the bracket with higher mechanical strength and inducibility. The method is simple and high in practicability.

The invention discloses a cartilage tissue engineering scaffold material and a preparation method thereof. The scaffold material is a three-dimensional porous structure formed by using sodium alginate and chitosan as a base material, and enabling the carboxyl on a side chain of the sodium alginate activated by activation cross-linking agent to react with amino group of chitosan, wherein molar ratio of the sodium alginate carboxyl to the chitosan amino group is (3:7)-(7:3), and the final product only contains two components, sodium alginate and chitosan. The three-dimensional porous scaffold provided by the invention has good biocompatibility, and is suitable for the cartilage tissue engineering.

The invention relates to three-dimensional porous engineering support material, comprising poly butylene succinate PBS and polycaprolactone PCL, wherein, the weight proportion is that the poly butylene succinate is 90 to 10 shares and the polycaprolactone is 10 to 90 shares. The invention also relates the preparation of the material, which is characterized in that the pore-foaming agent is arranged in a mold evenly and then the PBS/PLC blending chloroform solution is poured in; the support rudiment is put in a hood for 24 hours before immersing deionized water for 2 to 4 days; the PBS/PLC support material can be obtained after drying in vacuum. The invention relates to application of the material also, which mains that the material can be used as supports of bone or cartilage tissue engineered cells for repairing and restructuring the bone or cartilage tissue organ. The support material has the advantages of even inner hole structure and good communication between the holes, namely the hold diameter is between 10 to 500Mum and the porosity is between 70 to 91 percent, as well as the advantages of steady structure and easy preparation technology.

The invention discloses a tissue engineering scaffold material for repairing cartilage defects and a preparation method thereof. The tissue engineering scaffold material is prepared by the following steps of: cleaning the spongy bone at the shoulder blade of a fresh pig; cutting the spongy bone into small blocks with diameter of between 5 and 10mm and thickness of between 3 and 5mm; degreasing, decalcifying and deproteinizing; immersing with alcohol for 30 minutes; and air-drying, wherein the decalcifying time is 6 hours. The prepared demineralized spongy bone matrix (DSBM) has the characteristics of relatively simple and convenient preparation method, and capacity of being obtained on a large scale, can be stored for a long time at a low temperature and is economic and cheap. The material can be prepared into different shapes and sizes according to the shapes of the defects to be repaired, so the material is convenient to apply. The DSBM remains a natural netlike gap structural system of the pig spongy bone, has high cellular compatibility, biocompatibility and degradability and is a very good cartilage tissue engineering scaffold material.

The invention discloses a cartilage tissue engineering bracket and a preparation method thereof, and belongs to the field of cartilage repair. Components of the bracket comprise natural high polymer water-gel and decalcified bone matrix. By combining the natural high polymer water-gel with the decalcified bone matrix, the bracket not only has plasticity, adhesion and injection of the water-gel, but also has various advantages brought by the natural biological bracket material; and moreover, the decalcified bone matrix can provide a collagenous fiber net rack, and provides higher mechanical strength and inducibility for the prepared bracket, and further promotes adhesion, multiplication and differentiation of the cells. The invention further discloses the preparation method of the cartilage tissue engineering bracket, which comprises steps of injecting prepared natural high polymer water-gel liquor in the decalcified bone matrix, so that the natural high polymer water-gel is completely infiltrated into gaps of the decalcified bone matrix, and carrying out gelatinization reaction at a preset temperature to obtain the cartilage tissue engineering bracket with higher mechanical strength and higher inducibility. The method is simple, easy to operate and high in practicability.

The invention relates to a polycaprolactone/ polyethyleneglycol tissue engineering stent material and a preparation method thereof. The components of the material include: polycaprolactone and polyethylenelycol with the proportion of 100 to 0 to 40 to 60. The preparation method includes: (1) the polycaprolactone and the polyethylenelycol with a certain proportion are dissolved in cosolvent to obtain uniform transparent solution; (2) the solution is spun into a fiber felt of nano/secondary micron grade by the technique of electrostatic spinning; (3) a vacuum oven is vacuumed to remove the residual solvent of the fiber felt; (4) the fiber felt is took out, and is overlapped and filled in the mold with a specific shape; (5) the mold is put in the vacuum oven and is took out to be cooled for the UV sterilization after being heated at the constant temperature near the blend melting temperature. The stent material system of the invention has the advantages of stable structure, uniform structure of internal holes and is mutually communicated with holes and can be used as a cartilage tissue engineering cell stent.

The invention belongs to the technical field of regenerative medical materials, and discloses hyaluronic acid supramolecular hydrogel for three-dimensional culture of chondrocytes, and preparation andapplication of the hyaluronic acid supramolecular hydrogel. The method comprises the following steps: with water as a reaction medium, carrying out a copolymerization reaction on methacrylic anhydride modified hyaluronic acid, a double-bond functionalized ureido pyrimidinone functional monomer and 2-(2-methoxyethoxy)ethyl methacrylate under the action of an initiating system, and carrying out subsequent treatment to obtain the hyaluronic acid supramolecular hydrogel. The hydrogel is used in the field of cartilage tissue engineering, and is used for three-dimensional culture of chondrocytes and preparation of cartilage repair materials. The hydrogel disclosed by the invention has sol-gel conversion and self-repairing properties, uniform dispersion of the chondrocytes can be easily realizedby sol at a low temperature, and the sol can undergo phase change to form gel after being conveyed to a cartilage injury part, so in-situ fixation of the chondrocytes is realized; and the formation of a dynamic three-dimensional microenvironment of the hydrogel is beneficial for the maintenance of chondrocyte functions and the secretion of cartilage characteristic matrixes.

The invention belongs to the field of cartilage tissue engineering. The invention discloses a tissue engineering cartilage composite scaffold, which is constructed based on an extracellular matrix-derived cartilage micro-tissue, and a preparation method of the composite scaffold. Specifically, a cartilage extracellular matrix-derived micro-carrier is prepared from fresh cartilage through such processes as wet pulverization, sieving, decellularizing and the like; and the prepared micro-carrier can be used as a seed cell amplification vector. By virtue of a bioreactor, cartilage cells are rapidly amplified by the micro-carrier and the phenotype of the cartilage cells is kept; and meanwhile, the induced differentiation of stem cells towards the cartilage is also promoted and the cartilage micro-tissue is formed. By filling the cartilage micro-tissue in pores of a three-dimensional porous scaffold which is good in mechanical performance, the tissue engineering cartilage composite scaffold is constructed. The composite scaffold is good in mechanical performance, rich in natural cartilage extracellular matrix components and conducive to the induced differentiation of the stem cells towards the cartilage, and the composite scaffold is capable of providing a good microenvironment for the growth of seed cells and is expected to accelerate the in vivo construction speed of the tissue engineering cartilage.

The invention discloses a method for preparing a double-layer bionic cartilage tissue engineering scaffold, which comprises the following steps of: firstly, preparing a substrate; secondly, preparing the double-layer bionic cartilage tissue engineering scaffold on the surface of the substrate; and finally, soaking the double-layer bionic cartilage scaffold on the surface of the substrate by usingsolution of NaOH, and peeling the double-layer bionic cartilage scaffold from the substrate. The method has simple operation and high stability; the prepared double-layer bionic cartilage scaffold has a thin thickness and good mechanical properties and is favorable for clinical application; the scaffold is molded integrally; a vertical hole is tightly connected with a round hole to avoid the problem of breaking away from each other; the structure of the scaffold is similar to a natural cartilage structure and is hopeful to better maintain the phenotype of cartilage cells and improve the repairing effect on cartilage injuries; and the cartilage scaffold can be directly combined with a substrate comprising polylactic acid (PLA)/bone powder to form a bone cartilage scaffold used for repairing bone cartilage simultaneous colobomata, and a cartilage layer part still has a double-layer structure.

The invention discloses a preparation method of a silk fibroin-poly(hydroxybutyrate-hydroxyvalerate) composite fiber membrane, which comprises the steps of: mixing a prepared silk fibroin solution with a poly(hydroxybutyrate-hydroxyvalerate) solution to prepare a spinning solution, and conducting electrostatic spinning and aftertreatment. Based on the electrostatic spinning method, the composite fiber membrane is prepared, and the fiber membrane has good biocompatibility of the silk fibroin and the excellent mechanical performance of the poly(hydroxybutyrate-hydroxyvalerate) simultaneously, is more applicable to the biomedical field, and is expected to be used as a material for engineering of skin, nerves and cartilaginous tissues. The prepared composite fiber membrane has higher porosity, good mechanical performance, good air permeability and moisture permeability and large liquid absorption amount, can be degraded in human body and can promote tissue repair; and the preparation method has simple process.

The present invention provides a monoclonal antibody or a fragment thereof binding to the extracellular I-domain of integrin alpha10beta1 and a hybridoma cell line deposited at the Deutsche Sammlung von Microorganismen und Zellkulturen GmbH under the accession number DSM ACC2583. Furthermore, the present invention also provides a monoclonal antibody or a fragment thereof binding to the extracellular I-domain of integrin alpha10beta1 produced by the hybridoma cell line deposited. Method and uses of the antibody or a fragment thereof in identifying and selecting cells of a chondrogenic nature for treatment purposes, in particular for the identification and isolation of chondrocytes, mesenchymal progenitor cells and embryonic stem cells for tissue engineering of cartilage, or for identifying diagnostic and therapeutic tools in studying the biological role and the structural/functional relationships of the integrin alpha10beta1 with its various extracellular matrix ligands are also included.

The present invention uses L-lactic acid polymer whose molecular weight is 80000-200000 and calcium polyphosphate fibre whose mean length is 5-15 mm and adopts dissolving casting granule leaching process to obtain the invented skeleton material capable of being transplanted in the body. As compared with tissue engineering scaffold material of existent technology said invented product possesses high voidage, good biological degradable property and physical mechanical property, and possesses three-D communication, micropore and reticular microstructure, so that it can completely meet the basic requirements for tissue engineering.

The invention discloses a preparation technology of biphasic bone and cartilage tissue engineering scaffold material. Firstly chitosan and collagen powder are added into acetate solution and laid in a refrigerator to be preserved; then PLA solution is prepared, and bone dust is put into the PLA solution and is uniformly mixed and stirred; then prepared PLA/bone dust solution is injected into a die to be prefrozen; then collagen/chitosan solution is added into the prefrozen die to be prefrozen, and a scaffold sample is obtained after freeze drying; and finally the bone and cartilage biphasic scaffold conducted to freeze drying is taken out from the die. The preparation technology ensures that a bone layer scaffold has the hardness and the strength approximate to that of natural bone tissue, is not only beneficial to the repair of cartilage tissue, but also convenient for clinically operating and fixing the scaffold; and meanwhile, a cartilage layer scaffold is provided with a multilayer structure containing perpendicular pore canals, is convenient for guiding cells on the scaffold to grow at a state close to that of a natural cartilage laminated structure, and improves the injury repair effect of cartilage.

The invention discloses a preparation method of a silk fibroin/chitosan three-dimensional scaffold material, and belongs to the field of the preparation of a cartilage tissue engineering scaffold material. The invention provides a method for preparing the three-dimensional scaffold material from silk fibroin and chitosan. The method comprises the following steps: firstly, preparing the silk fibroin; and then, mixing and processing the silk fibroin and the chitosan, so that the silk fibroin/chitosan three-dimensional scaffold material is obtained. The silk fibroin/chitosan three-dimensional scaffold material prepared by the method conforms to the requirements on the physicochemical properties of the cartilage tissue engineering scaffold material; and the scaffold material is expected to become a relatively ideal scaffold material for cartilage tissue engineering.

The invention provides a separation culture method for cartilage stem cells. In the method, the cartilage stem cells are screened from cartilage tissue cells; fibroblast-like cells maintained under the culturing condition of a specific culture medium have the capabilities of differentiating bones, cartilages and fat; and compared with adult cells, the cartilage stem cells have good amplification capabilities, and can pass on for over 15 generations without changing the original characteristics after being obtained from a primary generation. In the method, the cartilage stem cells are obtained by screening and purifying under a specific culturing condition, so that the content of the cartilage stem cells is over 95 percent. A technology disclosed by the invention contributes to harvesting cartilage tissue specific stem cells, and can be applied to bone and cartilage tissue engineering.

The invention discloses a mechanical environment culture method for improving the proliferation activity of chondrocytes and maintaining phenotype of chondrocytes. The invention comprises the following steps: step 1, carrying out separation, culture and identification of chondrocytes; 2, inoculating the chondrocytes into a culture apparatus, loading the chondrocytes during the culture process, andculturing the chondrocyte in a mechanical environment so that the chondrocytes are mechanically stimulated. Under culture in mechanical environment, the proliferation of chondrocytes is improved, andco-cultured cells is promoted to have a good cartilage phenotype, which can quickly and effectively provide excellent seed cells for cartilage tissue engineering, and solve the clinical and practicalneeds of tissue engineering cartilage seed cells.

The invention discloses a growth-factor-chitosan-microsphere loaded DBM support joint cartilage repairing material. A method for preparing the growth-factor-chitosan-microsphere loaded DBM support joint cartilage repairing material includes the following steps: (1) preparing a DBM: adopting a fresh pig shoulder blade, removing the periosteum, the cartilage and the soft tissues, taking the cancellous bone of the shoulder blade, preparing the cancellous bone to be in a cake shape, carrying out repeated stirring and washing with flowing water to remove the marrow, the bloodiness and the surface grease, and after washing is carried out through the tap water, placing the cancellous bone into an environment at -80 DEG C for storage for three days to carry out proper processing; (2) preparing chitosan sustained release microspheres; (3) preparing wrapping growth factor microspheres, and calculating the encapsulation efficiency and the drug loading capacity; (4) preparing a growth-factor-chitosan-sustained-release-microsphere loaded DBM composite, and measuring amido bond connection after cross bonding is carried out a DBM support and the microspheres through EDC with the infrared ray method. The growth-factor-chitosan-microsphere loaded DBM support joint cartilage repairing material has the good cellular and biology compatibility and the good degradability, and is a good cartilage tissue engineering support material.

The present invention relates to a three-dimensional porous tissue engineering carrier material, its preparation and application. Said three-dimensional porous tissue engineering carrier material includes three-dimensional porous structure, its internal porous structure is uniform, three-dimensional through degree is above 90%, pore size is 50-500 micrometer and porosity is 65-90%. Its preparation method includes the following steps: using polyhydroxybutyrate valerate (PHBV) and polycaprolactone (PCL) as raw material, mixing them, melting and spinning to obtain fibre whose diameter is 10-500 micrometer, cutting said fibre to make said fibres have identical length, making said fibres be uniformly arranged and filled into a mould, sealing said mould for 5min-1n at 50-80deg.C, demoulding, sterilizing and packaging so as to obtain the invented product. Said product can be used for repairing or reconstructing some tissue organs.