The invention relates to the technical field of immune
cell preparation, and particularly discloses an NK
cell culture medium, an NK
cell and a culture method and application of the NK cell. The NK
cell culture medium comprises cell factors IL2, IL21 and TGF beta; the
system further comprises IL4. The NK cell obtaining method comprises the step of contacting cells with the culture medium. The NK cells obtained by the invention have relatively
high cell proliferation ability, and the proliferation ability,
motility and in-vivo effectiveness of the cells after
cryopreservation recovery are high.