15results about How to "Clear ingredients" patented technology

This invention discloses a compound preparation for treating vascular dementia and its preparation method. The active pharmaceutical ingredient consists of protopanaxadiol and gallic acid in a 1:1 mass ratio, and the dosage form is liposomes. The preparation method includes extraction, mixing, liposome preparation, and optional freeze-drying steps: protopanaxadiol and gallic acid are extracted and purified from ginseng rhizome and Terminalia chebula fruit, respectively. After mixing, they are combined with phospholipids and cholesterol to prepare a lipid membrane. The membrane is then homogenized by hydration, low-temperature ultrasound, and high-pressure microfluidic jet to obtain a homogeneous liposome suspension, which can be further freeze-dried into a freeze-dried powder. The 1:1 composition of protopanaxadiol and gallic acid targets multiple points on the pathological mechanism of vascular dementia, showing significant therapeutic effects and broad application prospects. This preparation uses a liposome dosage form to improve the bioavailability of the active ingredient, the preparation process parameters are controllable, the active ingredient has high purity, the liposomes have uniform particle size, high encapsulation efficiency, and good stability.

The application relates to the biological field, in particular to a lactobacillus fermentum zhangweizhuang cell lysate for regulating macrophage polarization and assisting PD-1 inhibitor to inhibit liver cancer development. The ZW18 cell lysate is a cell lysate containing various metabolites prepared from lactobacillus fermentum ZW18 with a preservation number of CGMCC No. 23549 through fermentation, heat treatment and ultrasonic lysis treatment, and the metabolites at least contain active ingredients: extracellular polysaccharide of greater than or equal to 2200 mg / L, amino acid of greater than or equal to 70 mmol / L and flavonoid of greater than or equal to 150 g / L. The ZW18 cell lysate can induce M1 type polarization of macrophages, can assist PD-1 inhibitor to inhibit liver cancer development in cooperation with the PD-1 inhibitor, can regulate intestinal flora imbalance caused by liver cancer, and can be used for preparing a combined preparation with the PD-1 inhibitor.

The invention relates to the technical field of cosmetics, in particular to a blue copper peptide-containing composition and application thereof in cosmetics. The composition comprises the following components in parts by mass: 1 part of blue copper peptide; 0.5 to 5 parts of tetrahydromethylpyrimidine carboxylic acid; 0.05 to 0.5 part of sodium hyaluronate; and 0.05 to 0.5 part of beta-glucan. Preferably, the composition further comprises allantoin and lactobionic acid, based on 1 part by mass of the blue copper peptide, the content of the allantoin is 0.1-0.5 part, the content of the lactobionic acid is 0.2-1.0 part, and the mass ratio of the allantoin to the lactobionic acid is 1: (2-4). In-vitro efficacy experiments prove that the specific combination and proportion can generate a remarkable synergistic effect, and meanwhile, the capabilities of inhibiting the activity of elastinase and promoting epidermal cell repair are improved, so that the excellent dual effects of wrinkle resistance and skin barrier repair are realized. The composition can be used for preparing cosmetics such as essence, emulsion and face cream.

The invention belongs to the technical field of cytobiology and immune cell therapy, and particularly relates to a gamma delta T cell serum-free additive culture medium composition with definite components and application and a product thereof. The serum-additive-free culture medium composition comprises a serum-additive-free culture medium A, a serum-free culture addition reagent B, a serum-additive-free culture medium C, a serum-additive-free culture medium D and a serum-additive-free culture medium E, the main components comprise ethanolamine, sodium selenite, recombinant human albumin, IL-15 and IL-2. According to the gamma delta T cell serum-free additive culture medium composition provided by the invention, the components are clear, the serum-free culture additives without animal-derived components can avoid potential safety hazards caused by the animal-derived components, the amplification efficiency is improved, the cell high activity is maintained, the GMP standard is met, and the gamma delta T cell serum-free additive culture medium composition is suitable for large-scale production and clinical transformation.

The application discloses application of dimethoxyphenol and a biological preparation thereof in plant pathogenic nematode control, and aims to solve the problems of single function, unknown active ingredient, unclear mechanism and unstable quality of existing microbial source preparations. The application accurately identifies 2,3-dimethoxyphenol as an important nematicidal active substance through metabolomics, and realizes stable co-accumulation of 2,3-dimethoxyphenol and a growth-promoting factor, isopentenyladenine, by combining with a specific strain (pink helical polysporal mold NF-06 and bacillus velezensis YB-1652) co-culture process, so that technical effects of clear composition, synergistic disease-preventing and growth-promoting function, controllable quality and environment friendliness are achieved.

The invention belongs to the technical field of traditional Chinese medicine modernization and medicine development, and particularly relates to a uric acid reducing product based on folium apocyni veneti extract and application of the uric acid reducing product. The core active component of the product is the folium apocyni veneti extract. In-vivo experiments prove that the extract can remarkably reduce the blood uric acid level and xanthine oxidase (XO) activity of a mouse with hyperuricemia. Six active ingredients entering blood are identified through in-vivo metabolism research. The direct interaction between the main active component and the target protein XO is verified from the molecular level through molecular docking, surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) technologies, and the uric acid reducing action mechanism of the compound is completely clarified. The invention provides a scientific basis and a high-quality product for developing a new uric acid-reducing drug effect of the folium apocyni veneti.

This invention belongs to the field of compositions of polymeric compounds for oral care products, specifically relating to a sustained-release system for oral care products, its preparation method, and its application. This invention discloses that when a cationic polymeric compound, sodium alginate, and ectoine are used in combination, a unique OraReliX™ sustained-release system can be formed on the tooth surface. The core working principle of this system is as follows: the cationic substance adheres to the negatively charged tooth surface through electrostatic adsorption, forming a cationic network; sodium alginate regulates the network density through polyelectrolyte complexation with this cationic network, while reversibly binding calcium ions; ectoine stabilizes the oral microenvironment and protects the sustained-release network structure. The synergistic effect of these three components significantly slows down the release rate of calcium ions, prolonging their effective retention time in the oral cavity.

The present application relates to the technical field of fermentation medium, more specifically, it relates to a high-density fermentation medium for streptococcus suis and preparation and application thereof.The medium comprises beef infusion powder 1-5 parts, tryptone 1-10 parts, pancreatic casein peptone 1-10 parts, soybean peptone 1-5 parts, dipotassium hydrogen phosphate 1-5 parts, sodium chloride 1-10 parts, yeast extract 1-10 parts and glucose 1-5 parts.

This invention relates to the field of deep processing technology of white hyacinth bean, specifically to a method for preparing a compound spleen-strengthening and dampness-removing white hyacinth bean paste. This invention utilizes low-temperature roasting to pretreat a specific ratio of dampness-removing medicinal herbs, including licorice, poria cocos, coix seed, and bitter almond, and prepares a compound decoction rich in dampness-removing components. This decoction is then used as a biological stressor to promote the germination of white hyacinth beans, with its concentration controlled to encourage moderate germination and the production of substances that resist adverse conditions. Simultaneously, leveraging the plant's water absorption capacity during germination, the invention incorporates the dampness-removing components of the compound decoction. Stable solid-state fermentation with pure Rhizopus further enhances the dampness-removing components of the compound white hyacinth bean and fermented soybean. This not only increases the content of major dampness-removing components such as polyphenols, proteins, and polysaccharides in the white hyacinth bean and fermented soybean, but also increases the content of trace dampness-removing substances such as rutin, quercetin, kaempferol, luteolin, and picolinic acid. It also promotes the dissolution of dampness-removing amino acids—alanine and glycine. The dampness-removing compound white hyacinth bean and fermented soybean components are clearly defined, and the content of spleen-strengthening and dampness-removing substances is richer.

The invention provides a vincristine sulfate injection and a preparation method thereof, and relates to the technical field of pharmaceutical preparations. The preparation method of the vincristine sulfate injection comprises the following steps: (1) adding vincristine sulfate and rosmarinic acid into an acetate buffer solution, uniformly stirring and mixing, filtering, collecting powder, drying, grinding and sieving to obtain a vincristine sulfate-rosmarinic acid eutectic; (2) adding mannitol into the water for injection for later use; and (3) adding the vincristine sulfate-rosmarinic acid eutectic into the system in the step (2), stirring, adjusting the pH value to 4.5-5.5, filtering with a microporous membrane, sterilizing, and filling to obtain the vincristine sulfate injection. The vincristine sulfate injection disclosed by the invention is simple in preparation process, good in reproducibility and easy for industrial production. The vincristine sulfate injection has the advantages of simple raw materials, no need of any synthetic carrier material, definite components and high safety, and the prepared vincristine sulfate injection has high stability and good application prospects.

The application discloses a kit for detecting cat feces related circular virus and application thereof, and belongs to the technical field of DNA virus detection. The detection kit can significantly improve the detection flux and timeliness of the cat feces related circular virus, has high detection sensitivity and strong specificity, can effectively solve the problem of low FeSCV virus load in healthy cats and easy missed detection, has clear components, low device dependency, and the color change can be directly observed by naked eyes under natural light to read the result, greatly simplifies the operation process, reduces the personnel operation error, and improves the reliability of the detection result, has low requirement on professional knowledge, is particularly suitable for use in primary pet clinics, cat houses / farms, isolation quarantine areas and field epidemiological investigation, can simultaneously detect multiple geographical strains of FeSCV such as Chinese epidemic strains and Japanese reference strains, can realize consistent detection of different geographical strains, and provides a reliable tool for the epidemiological monitoring of FeSCV.

The present application provides a Cistanche deserticola polysaccharide and Hippophae rhamnoides polypeptide composition with hypoxia tolerance activity, and the amino acid sequence of the Hippophae rhamnoides polypeptide is SEQ ID NO:1. The composition provided by the present application can significantly prolong the survival time of mice, is superior to single components and positive controls, and exhibits synergistic effect. Molecular docking analysis shows that the used polypeptide has high affinity with SIRT1, supporting its mechanism in optimizing cell energy metabolism and antioxidant stress. The composition is suitable for highland medical protection and adjuvant treatment of hypoxic diseases, and has the advantages of clear components and stable biological activity.

The application provides a serum-free cryopreservation solution and application thereof in cell cryopreservation. The serum-free cryopreservation solution comprises a first additive, a saccharide, an inorganic salt, a protein and a second additive; in the serum-free cryopreservation solution, the mass concentration of the first additive is 0.2-15% (w / v), the concentration of the saccharide is 2-15 mg / mL, the mass concentration of the inorganic salt is 0.7-1.2% (w / v), the concentration of the protein is 0.05-0.5 g / mL, and the concentration of the second additive is 0.5-5 mg / mL. The serum-free cryopreservation solution provided by the application ensures that the cell still has high survival rate, high activity and functional integrity after cryopreservation through the combined action of the above components, the composition of the cryopreservation solution is clear, and no animal-derived serum needs to be added, so that the immunogenicity risk, pathogen contamination and batch difference caused by the animal-derived serum are avoided, and the safety of the cryopreservation solution is significantly improved.

The present application relates to the technical field of functional active substance extraction, and particularly relates to extraction of pearl polypeptide with antioxidant effect. The present application adopts ultrasonic-assisted acetic acid extraction and Sephadex G-25 gel filtration column separation and purification to prepare pearl polypeptide extract. The obtained pearl polypeptide extract contains peptide segments with amino acid sequences as shown in SEQ ID NO. 1-5. Analysis and determination show that the pearl polypeptide extract of the present application has significant antioxidant capacity and can effectively scavenge hydroxyl radicals and ABTS free radicals at low concentration. In addition, the pearl polypeptide extract prepared by the present application has high extraction rate and purity and can be widely used in the fields of food, biological medicine and cosmetics.

The invention belongs to the technical field of nutritional compositions and cosmetics, and particularly relates to a hydroxytyrosol composition and application thereof in anti-aging products. The hydroxytyrosol composition disclosed by the invention is prepared from pyrroloquinoline quinone and hydroxytyrosol. The extracellular matrix (ECM) is a core basis for maintaining the structure and form of the skin, the collagen is a core component of the ECM, and the hydroxyproline is a characteristic amino acid of the collagen; the TGF-beta1 and the MMP-1 are used as key factors of senescence-related secretory phenotypes to regulate and control ECM remodeling and skin senescence. The hydroxytyrosol composition can improve the contents of collagen, hydroxyproline and TGF-beta1 and promote ECM synthesis and skin repair; mMP-1 expression is inhibited, and collagen degradation is reduced; the MDA level is reduced, and the oxidation resistance is enhanced; meanwhile, the moisture content and the hyaluronic acid content of the skin are increased, the moisturizing performance is improved, the components are definite, the quality is controllable, skin aging can be delayed in a multi-target mode, and good application prospects are achieved in the anti-aging field.