43results about How to "Clear ingredients" patented technology

The invention belongs to the technical field of anti-aging products, and particularly relates to an anti-aging composition as well as a preparation method and application thereof. The anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 0.5%-1% of xanthophyll, 0.1%-0.3% of pyrroloquinoline quinone, 0.01%-0.02% of sodium propyl paraben, 0.5%-1% of an emulsifier and the balance of water. The emulsifier is a non-ionic emulsifier. The anti-aging composition prepared by the invention has definite components, is free of allergic reactions such as skin redness, rash and itching through tests of multiple testees, and is milder and significantly reduced in sensitization rate compared with products in the prior art.

The invention discloses a culture medium and a culture method for inducing olfactory mucosa mesenchymal stem cells into olfactory ensheathing cells, and belongs to the technical field of cell induction and culture. The main components of the culture medium provided by the invention are cell nutritional factors and do not contain animal proteins, and all the components act together, so that a suitable growth and differentiation environment is provided for the olfactory mucosa mesenchymal stem cells. The invention also provides a method for inducing the olfactory mucosa mesenchymal stem cells into the olfactory ensheathing cells by using the culture medium. According to the culture method provided by the invention, the mesenchymal stem cells can be induced into the olfactory ensheathing cells in stages. The method is simple, convenient, safe and efficient, the induced cell expresses the olfactory ensheathing cell marker, the problem of the source of the olfactory ensheathing cell is solved, and the method has a good application prospect in the field of neural restoration.

The invention relates to the technical field of stem cell induced differentiation, and discloses an umbilical cord mesenchymal stem cell adipogenic induced differentiation culture medium and a culture method. The culture medium comprises the following substances: a DMEM (Dulbecco's Modified Eagle Medium) / F12 basal culture medium, fetal calf serum, insulin, penicillin, streptomycin, dexamethasone, 3-isobutyl-1-methylxanthine, indometacin, BMP-2 and FGF-1 (Fibroblast Growth Factor 1). The culture method comprises the following steps: (1) carrying out primary isolation culture on umbilical cord mesenchymal stem cells; (2) carrying out passage on the umbilical cord mesenchymal stem cells; and (3) inducing adipogenesis of the umbilical cord mesenchymal stem cells by using the adipogenesis induced differentiation culture medium. BMP-2 and FGF-1 are added on the basis of a traditional adipogenic induction culture medium to induce and promote the umbilical cord mesenchymal stem cells to be more efficiently differentiated into adipocytes under the combined action, so that the adipogenic differentiation time is shortened, and the adipogenic differentiation efficiency is improved; the umbilical cord mesenchymal stem cell adipogenic culture medium is stable in formula, simple and convenient in culture method and suitable for wide popularization.

The invention relates to the technical field of organoid culture, in particular to tumor organoid culture hydrogel with definite components and a preparation method and application thereof, and the tumor organoid culture hydrogel with the definite components comprises a gel precursor and laminin 521; the gel precursor is prepared by dissolving sodium alginate and gelatin in PBS; the concentration of the laminin 521 is 0.10 mg / ml to 0.15 mg / ml. The hydrogel provided by the invention is clear in component, the performance of the hydrogel for culturing the tumor organoid is equivalent to that of Matrigel, and the technical problems that Matrigel is complex in component, different in batches and high in price, and consequently conversion and application of a tumor patient source organoid model are limited are solved. Besides, the hydrogel provided by the invention is high in component industrialization degree, good in stability and easy to obtain, standardized and economic production can be realized, and support is provided for construction of a tumor organ model.

The invention relates to the field of natural medicines, and particularly discloses Tibetan medicine scindapsus aureus flower polysaccharide A1 with a lipid-lowering function as well as a preparation method and application thereof. The Tibetan medicine scindapsus aureus flower polysaccharide A1 is prepared from the following components in mol percent: 55 to 60 percent of arabinose, 15 to 20 percent of galactose, 5 to 10 percent of galacturonic acid, 5 to 10 percent of rhamnose and 3 to 5 percent of glucose. The Tibetan medicine scindapsus aureus flower polysaccharide A1 is extracted and separated from Tibetan medicine scindapsus aureus flowers. The method comprises the following steps: S1, extracting and preparing Tibetan medicine scindapsus aureus flower total polysaccharide by adopting a water extraction and alcohol precipitation method; s2, separating and purifying the scindapsus aureus flower total polysaccharide by adopting an anion exchange chromatography technology to obtain EGP-A, and further separating and purifying the EGP-A by adopting a gel column chromatography technology to obtain EGP-A1. Through research and implementation of the invention, the EGP-A1 has good lipid-lowering activity, can play a role in protecting blood fat and cardiovascular health, and has a good medicinal prospect in the aspect of preparing lipid-lowering medicines.

The invention discloses a plant genome DNA (deoxyribonucleic acid) extraction reagent. The plant genome DNA extraction reagent comprises a Buffer 1 lysis solution, a Buffer 2 precipitation solution and a Buffer 3 binding solution, the Buffer 1 lysate comprises sodium chloride, PVP40 (Polyvinyl Pyrrolidone 40), sodium metabisulfite and SDS (Sodium Dodecyl Sulfate); wherein the concentration of the sodium chloride is 250 to 1200 mM; the mass percent of the PVP40 is 0.5%-3%; the concentration of the sodium metabisulfite is 50-150mM; the mass percent of the SDS is 0.7%-2.5%; the Buffer 2 precipitation solution comprises acetate; wherein the concentration of the acetate is 2.2 to 4.3 M; the Buffer 3 binding solution comprises guanidine hydrochloride; wherein the concentration of the guanidine hydrochloride is 0.5 to 2.5 M. The extraction reagent does not contain organic solvents such as phenol and chloroform, and the removal rate of polyphenol can be improved.

The invention discloses a culture medium system for inducing airway stem cells to differentiate into airway epithelial cells based on ALI and application of the culture medium system. Belongs to the technical field of ALI culture. By means of the culture medium system, the airway stem cells can be efficiently and stably induced to be differentiated into multiple types of airway epithelial cells in the ALI culture process, and particularly, the generated cilia cells are close to the physiological state in vivo. Compared with an imported culture medium, the culture medium has the advantages of definite components, high batch stability, low cost and simple preparation method; the generated airway epithelial cells have a plurality of cell lineages, can well simulate airway epithelium, and show forms and functional characteristics similar to those of an in-vivo airway. According to the system, a respiratory tract epithelium-induced ALI culture system is remarkably improved, the research cost is reduced, a preferable scheme is provided for scholars engaged in studying physiological and pathological characteristics of respiratory tract epithelial cells based on ALI, and researchers are helped to establish a stable repeatable airway model.

The invention belongs to the field of medicines, and particularly relates to application of odd-carbon saturated medium and long-chain fatty acid in preparation of a medicine for treating polyglutamine diseases. Research results of the invention show that the heptadecanoic acid can significantly delay and inhibit the aggregation of polyglutamine (polyQ) disease proteins, and can significantly prolong the survival time of AM141 nematodes at the same time, and the prolonging effect is about 10%. Therefore, the heptadecanoic acid is completely possible to be used as a raw material to prepare related medicines, so as to treat polyQ diseases. In addition, the heptadecanoic acid is clear in component, low in price and easy to obtain, and is more beneficial to benefiting the public.

This invention discloses a compound preparation for treating vascular dementia and its preparation method. The active pharmaceutical ingredient consists of protopanaxadiol and gallic acid in a 1:1 mass ratio, and the dosage form is liposomes. The preparation method includes extraction, mixing, liposome preparation, and optional freeze-drying steps: protopanaxadiol and gallic acid are extracted and purified from ginseng rhizome and Terminalia chebula fruit, respectively. After mixing, they are combined with phospholipids and cholesterol to prepare a lipid membrane. The membrane is then homogenized by hydration, low-temperature ultrasound, and high-pressure microfluidic jet to obtain a homogeneous liposome suspension, which can be further freeze-dried into a freeze-dried powder. The 1:1 composition of protopanaxadiol and gallic acid targets multiple points on the pathological mechanism of vascular dementia, showing significant therapeutic effects and broad application prospects. This preparation uses a liposome dosage form to improve the bioavailability of the active ingredient, the preparation process parameters are controllable, the active ingredient has high purity, the liposomes have uniform particle size, high encapsulation efficiency, and good stability.

The present invention discloses a printable liquid metal ink and a preparation method thereof, the preparation method comprising the following steps: 1) taking a gallium-indium alloy and placing it in a relatively high concentration of hydrochloric acid, ensuring that the hydrochloric acid can completely cover the gallium-indium alloy, and incubating overnight; 2) washing with anhydrous ethanol, washing three times to ensure that the hydrochloric acid is completely removed, adding a para-aniline derivative and nano-clay, and mixing until dissolved; 3) using an ultrasonic cell disruptor for ultrasonic modification; 4) washing the liquid metal nanoparticles obtained in step 3) with water; 5) dissolving a polyethylene copolymer in water; 6) resuspending the liquid metal particles in step 4) with deionized water, and then mixing with the ink carrier obtained in step 4) to obtain liquid metal ink. The present invention adopts a physical-chemical combination method for compound modification, and the obtained liquid metal ink has high viscosity and strong adhesion after mixing with the ink carrier, and can effectively adhere to a flexible substrate to form a conductive electrode.

The present disclosure relates to a mulberry extract used in preparation of a drug for reducing animal weight. The mulberry extract comprises: 3% to 99% of an alkaloid, 0.2% to 70% of a polysaccharide, 0% to 50% of an amino acid, 0% to 10% of flavone, and 0% to 25% of other components. The mulberry extract of the present disclosure can be used for controlling weight gain, and has a good therapeutic effect on type 2 diabetes with obesity.

The invention discloses a kind of bovine adipose mesenchymal stem cell serum-free culture medium suitable for cell culture meat, belongs to the field of animal cell culture technology, including DMEM basic culture, L-alanyl-L-glutamine, recombinant transferrin, recombinant bovine insulin, sodium selenite, zinc sulfate heptahydrate, cortisol, recombinant human albumin, recombinant basic fibroblast growth factor, recombinant transforming growth factor β1, recombinant insulin-like growth factor, ethanolamine, mercaptoethanol and 2-phosphoric acid-L-ascorbic acid sodium salt. The inventor of the present application has obtained a new bovine adipose-derived stem cell (Adipose-derived stem cell, ADSC) serum-free culture medium through repeated experimental verification, which can effectively culture bovine adipose mesenchymal stem cells.

The application relates to the biological field, in particular to a lactobacillus fermentum zhangweizhuang cell lysate for regulating macrophage polarization and assisting PD-1 inhibitor to inhibit liver cancer development. The ZW18 cell lysate is a cell lysate containing various metabolites prepared from lactobacillus fermentum ZW18 with a preservation number of CGMCC No. 23549 through fermentation, heat treatment and ultrasonic lysis treatment, and the metabolites at least contain active ingredients: extracellular polysaccharide of greater than or equal to 2200 mg / L, amino acid of greater than or equal to 70 mmol / L and flavonoid of greater than or equal to 150 g / L. The ZW18 cell lysate can induce M1 type polarization of macrophages, can assist PD-1 inhibitor to inhibit liver cancer development in cooperation with the PD-1 inhibitor, can regulate intestinal flora imbalance caused by liver cancer, and can be used for preparing a combined preparation with the PD-1 inhibitor.

The invention relates to the technical field of cosmetics, in particular to a blue copper peptide-containing composition and application thereof in cosmetics. The composition comprises the following components in parts by mass: 1 part of blue copper peptide; 0.5 to 5 parts of tetrahydromethylpyrimidine carboxylic acid; 0.05 to 0.5 part of sodium hyaluronate; and 0.05 to 0.5 part of beta-glucan. Preferably, the composition further comprises allantoin and lactobionic acid, based on 1 part by mass of the blue copper peptide, the content of the allantoin is 0.1-0.5 part, the content of the lactobionic acid is 0.2-1.0 part, and the mass ratio of the allantoin to the lactobionic acid is 1: (2-4). In-vitro efficacy experiments prove that the specific combination and proportion can generate a remarkable synergistic effect, and meanwhile, the capabilities of inhibiting the activity of elastinase and promoting epidermal cell repair are improved, so that the excellent dual effects of wrinkle resistance and skin barrier repair are realized. The composition can be used for preparing cosmetics such as essence, emulsion and face cream.

The invention provides a feed additive and application, and belongs to the technical field of feed additives. The invention provides a feed additive which can be applied to preparation of feed for promoting weight gain of mutton sheep and preventing enteritis. The feed additive is a compound traditional Chinese medicine preparation, the components of the feed additive are clear, easy to obtain and simple to prepare, all the components are definite effective components extracted from Chinese herbal medicines, and the feed additive has good prevention and treatment effects on inflammatory diarrhea easily generated in the fattening stage of weaned lambs. The problems of drug resistance and drug residues caused by western medicines in the prior art are solved, and the problems of unclear effective components and unknown content of compound traditional Chinese medicines in the prior art are also solved.

The invention belongs to the technical field of cytobiology and immune cell therapy, and particularly relates to a gamma delta T cell serum-free additive culture medium composition with definite components and application and a product thereof. The serum-additive-free culture medium composition comprises a serum-additive-free culture medium A, a serum-free culture addition reagent B, a serum-additive-free culture medium C, a serum-additive-free culture medium D and a serum-additive-free culture medium E, the main components comprise ethanolamine, sodium selenite, recombinant human albumin, IL-15 and IL-2. According to the gamma delta T cell serum-free additive culture medium composition provided by the invention, the components are clear, the serum-free culture additives without animal-derived components can avoid potential safety hazards caused by the animal-derived components, the amplification efficiency is improved, the cell high activity is maintained, the GMP standard is met, and the gamma delta T cell serum-free additive culture medium composition is suitable for large-scale production and clinical transformation.

The application discloses application of dimethoxyphenol and a biological preparation thereof in plant pathogenic nematode control, and aims to solve the problems of single function, unknown active ingredient, unclear mechanism and unstable quality of existing microbial source preparations. The application accurately identifies 2,3-dimethoxyphenol as an important nematicidal active substance through metabolomics, and realizes stable co-accumulation of 2,3-dimethoxyphenol and a growth-promoting factor, isopentenyladenine, by combining with a specific strain (pink helical polysporal mold NF-06 and bacillus velezensis YB-1652) co-culture process, so that technical effects of clear composition, synergistic disease-preventing and growth-promoting function, controllable quality and environment friendliness are achieved.

The invention belongs to the technical field of traditional Chinese medicine modernization and medicine development, and particularly relates to a uric acid reducing product based on folium apocyni veneti extract and application of the uric acid reducing product. The core active component of the product is the folium apocyni veneti extract. In-vivo experiments prove that the extract can remarkably reduce the blood uric acid level and xanthine oxidase (XO) activity of a mouse with hyperuricemia. Six active ingredients entering blood are identified through in-vivo metabolism research. The direct interaction between the main active component and the target protein XO is verified from the molecular level through molecular docking, surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) technologies, and the uric acid reducing action mechanism of the compound is completely clarified. The invention provides a scientific basis and a high-quality product for developing a new uric acid-reducing drug effect of the folium apocyni veneti.

The invention discloses a skin care composition for removing acnes and fading marks as well as a preparation method and application of the skin care composition. The composition is prepared from the following raw materials in parts by weight: 0.5 to 1.5 g of fructus gardeniae, 0.8 to 1.3 g of radix scutellariae, 0.8 to 1.5 g of radix et rhizoma rhei, 0.6 to 1.4 g of rhizoma coptidis, 0.8 to 1.3 g of radix platycodonis, 0.5 to 1.4 g of herba menthae, 0.6 to 1.3 g of cortex phellodendri, 0.7 to 1.5 g of liquorice root and 1.5 to 2.5 g of flos lonicerae. The preparation method of the composition comprises the following steps: step 1) weighing the raw materials in parts by weight, cleaning and drying; step 2) crushing the dried raw materials, and sieving with a 100-mesh sieve to obtain crushed raw materials; and step 3) uniformly mixing the crushed raw materials, and filling the mixture into a special non-woven fabric bag to prepare a bagged material. The composition adopts natural traditional Chinese medicinal materials as raw materials, is clear in components, has a remarkable acne removing and mark fading effect, and can effectively remove acnes, blackheads, comedos and grease dirt and prevent skin blockage; the mask can be used for personal facial care, and has a better using effect in combination with a cold and hot spraying instrument, professional acne removal and acne removal techniques and an oxygen injection instrument.

The invention relates to a functional sports blast bead candy, which consists of a capsule shell and a content sealed in the capsule shell, and is characterized in that the capsule shell consists of the following components in parts by weight: 20-60 parts of gelatin, 15-60 parts of a plasticizer, 1-25 parts of a humidifying agent, 5-40 parts of water, and optional proper amounts of edible essence, a sweetening agent, a coloring agent and a thickening agent; the content is prepared from the following raw materials in parts by weight: 1-99 parts of active ingredients and 1-99 parts of auxiliary materials. The functional sports blast bead candy has the advantages of supplementing energy and being convenient to carry. The active component is a traditional Chinese medicine composition for resisting fatigue and is prepared from the following raw materials in parts by mass: 20 parts of astragalus membranaceus, 15 parts of rhizoma polygonati, 8 parts of cinnamon, 6 parts of uncaria and 6 parts of mint.

This invention belongs to the field of compositions of polymeric compounds for oral care products, specifically relating to a sustained-release system for oral care products, its preparation method, and its application. This invention discloses that when a cationic polymeric compound, sodium alginate, and ectoine are used in combination, a unique OraReliX™ sustained-release system can be formed on the tooth surface. The core working principle of this system is as follows: the cationic substance adheres to the negatively charged tooth surface through electrostatic adsorption, forming a cationic network; sodium alginate regulates the network density through polyelectrolyte complexation with this cationic network, while reversibly binding calcium ions; ectoine stabilizes the oral microenvironment and protects the sustained-release network structure. The synergistic effect of these three components significantly slows down the release rate of calcium ions, prolonging their effective retention time in the oral cavity.

The invention provides a mycoplasma hyopneumoniae culture medium capable of remarkably improving strain titer and application of the mycoplasma hyopneumoniae culture medium, and belongs to the field of veterinary biological products. The mycoplasma hyopneumoniae culture medium is obtained by adding L-asparagine, myo-inositol, arachidonic acid, urea, histamine, sodium pyruvate, valproic acid, glyceric acid, lysophosphatidic acid, L-citrulline, L-glutamine and L-leucine into a KM2 culture medium containing Bama miniature pig serum. According to the culture medium disclosed by the invention, the growth speed and the viable bacterium titer of the mycoplasma hyopneumoniae can be remarkably improved under the condition of low serum concentration, and the production cost is reduced.

The present invention belongs to the technical field of non-antibiotic treatment of bacterial infection, and in particular, relates to a fully active nano drug that synergistically and step by step combats bacterial biofilm infection, and its preparation method and application. The fully active nano preparation includes berberine, tetraphenylporphyrin tetrasulfonic acid and lanthanum nitrate. The drug releases drugs specifically in the biofilm microenvironment, targets lanthanum ions to break the bacterial intracellular drug delivery barrier, TPPP-mediated sonodynamic therapy to strongly kill bacterial cells, and berberine efficiently interferes with bacterial quorum sensing, so as to synergize step by step and programmatically combat the biofilm of drug-resistant bacteria. The preparation process is simple and does not require the introduction of a carrier. The obtained preparation has clear ingredients, each component is used to its full potential, and the proportion of active drugs is as high as 100%. It can effectively overcome the limitations of traditional carrier drug delivery nano preparations and has a high potential for clinical transformation.

The present application relates to the technical field of fermentation medium, more specifically, it relates to a high-density fermentation medium for streptococcus suis and preparation and application thereof.The medium comprises beef infusion powder 1-5 parts, tryptone 1-10 parts, pancreatic casein peptone 1-10 parts, soybean peptone 1-5 parts, dipotassium hydrogen phosphate 1-5 parts, sodium chloride 1-10 parts, yeast extract 1-10 parts and glucose 1-5 parts.

This invention relates to the field of deep processing technology of white hyacinth bean, specifically to a method for preparing a compound spleen-strengthening and dampness-removing white hyacinth bean paste. This invention utilizes low-temperature roasting to pretreat a specific ratio of dampness-removing medicinal herbs, including licorice, poria cocos, coix seed, and bitter almond, and prepares a compound decoction rich in dampness-removing components. This decoction is then used as a biological stressor to promote the germination of white hyacinth beans, with its concentration controlled to encourage moderate germination and the production of substances that resist adverse conditions. Simultaneously, leveraging the plant's water absorption capacity during germination, the invention incorporates the dampness-removing components of the compound decoction. Stable solid-state fermentation with pure Rhizopus further enhances the dampness-removing components of the compound white hyacinth bean and fermented soybean. This not only increases the content of major dampness-removing components such as polyphenols, proteins, and polysaccharides in the white hyacinth bean and fermented soybean, but also increases the content of trace dampness-removing substances such as rutin, quercetin, kaempferol, luteolin, and picolinic acid. It also promotes the dissolution of dampness-removing amino acids—alanine and glycine. The dampness-removing compound white hyacinth bean and fermented soybean components are clearly defined, and the content of spleen-strengthening and dampness-removing substances is richer.

The invention provides a vincristine sulfate injection and a preparation method thereof, and relates to the technical field of pharmaceutical preparations. The preparation method of the vincristine sulfate injection comprises the following steps: (1) adding vincristine sulfate and rosmarinic acid into an acetate buffer solution, uniformly stirring and mixing, filtering, collecting powder, drying, grinding and sieving to obtain a vincristine sulfate-rosmarinic acid eutectic; (2) adding mannitol into the water for injection for later use; and (3) adding the vincristine sulfate-rosmarinic acid eutectic into the system in the step (2), stirring, adjusting the pH value to 4.5-5.5, filtering with a microporous membrane, sterilizing, and filling to obtain the vincristine sulfate injection. The vincristine sulfate injection disclosed by the invention is simple in preparation process, good in reproducibility and easy for industrial production. The vincristine sulfate injection has the advantages of simple raw materials, no need of any synthetic carrier material, definite components and high safety, and the prepared vincristine sulfate injection has high stability and good application prospects.

The invention discloses a dietary fiber composition for improving immunity and intestinal health and application thereof, and belongs to the technical field of functional foods.The dietary fiber composition is composed of asparagus powder, licorice powder, loquat powder, avocado powder and sea buckthorn powder, and the weight ratio of the loquat powder to the avocado powder to the sea buckthorn powder is (1-10): (1-10): (0.5-3). The dietary fiber composition composed of the asparagus powder, the licorice powder, the loquat powder, the avocado powder and the sea buckthorn powder has the effects of enhancing immunity, regulating intestinal flora functions and relaxing bowel. Experiments find that when the weight ratio of the loquat powder to the avocado powder to the sea buckthorn powder is (1-10): (1-10): (0.5-3), the dietary fiber composition has a synergistic effect in the aspect of enhancing immunity.

The application discloses a kit for detecting cat feces related circular virus and application thereof, and belongs to the technical field of DNA virus detection. The detection kit can significantly improve the detection flux and timeliness of the cat feces related circular virus, has high detection sensitivity and strong specificity, can effectively solve the problem of low FeSCV virus load in healthy cats and easy missed detection, has clear components, low device dependency, and the color change can be directly observed by naked eyes under natural light to read the result, greatly simplifies the operation process, reduces the personnel operation error, and improves the reliability of the detection result, has low requirement on professional knowledge, is particularly suitable for use in primary pet clinics, cat houses / farms, isolation quarantine areas and field epidemiological investigation, can simultaneously detect multiple geographical strains of FeSCV such as Chinese epidemic strains and Japanese reference strains, can realize consistent detection of different geographical strains, and provides a reliable tool for the epidemiological monitoring of FeSCV.

The present invention belongs to the field of bioengineering technology, and specifically relates to a kit and a detection method for detecting respiratory syncytial virus infection. The present invention first provides a respiratory syncytial virus F recombinant protein, which is based on the respiratory syncytial virus F wild protein sequence, replaces the Furin restriction site and p27 polypeptide between the F1 fragment and the F2 fragment of the wild protein sequence with a (Gly)8 flexible connecting peptide sequence, and optimizes the codons. Then, an application based on the recombinant protein is provided.

The present invention discloses a hyaluronic acid or salt composition and its application for improving body immunity and protecting gastrointestinal health. The composition containing hyaluronic acid or salt thereof comprises three molecular weight hyaluronic acids or salts thereof. The composition of the present invention can enhance immunity, relieve intestinal inflammation, promote intestinal peristalsis, and regulate intestinal flora through synergistic enhancement, and can be added to food and health food as an effective ingredient, or used as an excipient in combination with drugs.