100 results about "Lactobionic acid" patented technology

The invention relates to the field of biomedical materials, in particular to a composite hydrogel microsphere with excellent cell adhesion performance and a preparation method and application thereof, and the preparation method comprises the following steps: (1) modifying silk fibroin with glutathione to obtain sulfydryl modified silk fibroin SF-GSH; (2) adopting methacrylic anhydride as a double-bond introduction reagent, introducing a methacrylate group on hyaluronic acid and forming a carbon-carbon double bond to obtain double-bond modified hyaluronic acid HA-MA; (3) mixing the sulfydryl modified silk fibroin SF-GSH with double bond modified hyaluronic acid HA-MA, and adding lactobionic acid to prepare a composite hydrogel microsphere precursor solution; and (4) preparing the composite hydrogel microsphere precursor solution into the composite hydrogel microsphere containing lactobionic acid. Compared with single hyaluronic acid hydrogel, the composite hydrogel microspheres prepared by the preparation method provided by the invention have good biocompatibility.

The invention discloses a composition for improving the stability of oleuropein in a solution as well as a preparation method and application of the composition. The composition comprises oleuropein and at least one stabilizer, and the stabilizer is selected from the group consisting of anisic acid, lactobionic acid, glycyrrhizic acid, 3, 3-thiodipropionic acid, N-acetyl-L-glutamine, propyl-cysteine and lauramidopropyl betaine; and the pH value of the aqueous solution of the composition is 4.0-8.0. The preparation method comprises the following steps: dissolving oleuropein in water, adding the stabilizer, and uniformly mixing. Through simple physical mixing, by utilizing the synergistic effect of the specific stabilizer and the oleuropein, the degradation and discoloration of the oleuropein in the storage process are remarkably inhibited. Experiments show that after the preferable composition is preserved for 30 days in an open manner, the retention rate of the oleuropein still reaches up to 80% or above, and is far superior to that of blank control and a traditional liposome wrapping method. The composition is simple and convenient in preparation process and low in cost, and provides reliable technical support for wide application of oleuropein in liquid products such as food, medicine and cosmetics.

The invention discloses an oil-controlling and acne-removing synergistic composition which comprises the following components in percentage by weight: 0.8 to 2.0 percent of succinic acid, 0.3 to 1.0 percent of salicylic acid derivative and 0.5 to 1.5 percent of PCA (Principal Component Analysis) zinc. The invention also provides a sustained-release body. The sustained-release body is prepared from the following components in percentage by weight: 0.8 to 2.0 percent of succinic acid, 0.3 to 1.0 percent of salicylic acid derivative, 0.5 to 1.5 percent of PCA zinc, 1.0 to 2.5 percent of lactobionic acid, 1.0 to 3.0 percent of lactobacillus fermentation product, 2.0 to 4.0 percent of aminated modified diatomite and 0.1 to 0.3 percent of citric acid. The invention also provides a preparation method and application of the sustained release body. The acne-removing shampoo has low irritation, and can be used for controlling oil and removing acnes for a long time and efficiently dredging hair follicles.

The invention discloses a glycosylated quercetin amphiphilic prodrug, a preparation method and application of the glycosylated quercetin amphiphilic prodrug. The glycosylated quercetin amphiphilic prodrug, the preparation method of the glycosylated quercetin amphiphilic prodrug and the application of the glycosylated quercetin amphiphilic prodrug are included. According to the glycosylated quercetin amphiphilic prodrug as well as the preparation method and the application thereof, the pH-sensitive glycosylated quercetin amphiphilic prodrug is obtained through parallel connection of the boric acid ester and the hydrophilic targeting sugar molecule lactobionic acid and the hydrophobic quercetin, and the pH-sensitive glycosylated quercetin amphiphilic prodrug and clinical common anti-tumor drugs are further assembled into a nano-drug; the invention aims at improving the physicochemical / pharmacological activity of quercetin and exploring the anti-tumor effect of quercetin cooperating with clinical drugs at the same time. The glycosylated quercetin amphiphilic prodrug is designed according to a drug combination principle, a formula 3 is used as a substrate, molecular quercetin is combined to obtain the glycosylated quercetin amphiphilic prodrug, and pH-sensitive borate bonds are introduced, so that drug release and tumor resistance can be controlled, the drug has certain targeting property, and the biological activity is remarkably improved.

The present disclosure describes compositions and methods including liposome or lipid nanoparticles (LNPs) including carbohydrate-based ligands that target the liver, lungs, and / or kidneys. More particularly, the present disclosure relates to compositions and methods including liposome or lipid nanoparticles (LNPs) including carbohydrate-based ligands, such as di-lactobionic acid (LBA) ligand, and a suitable cargo such as nucleic acids and small molecule to target the liver, lungs, and / or kidneys for treatment and / or diagnosis of diseases and conditions.

The invention relates to a polyacid composite oil-controlling and acne-removing gel and a preparation method thereof, and belongs to the technical field of skin care products. The polyacid composite oil-controlling and acne-removing gel is prepared from the following components in percentage by mass: 2% of salicylic acid, 3%-4% of glycolic acid, 0.5%-1.5% of mandelic acid, 0.5%-1.0% of lactobionic acid, 0.5%-1.0% of gluconolactone, 1.2%-1.6% of microcrystalline cellulose (and) sphingomonas fermentation product extract (and) cellulose gum, 2%-5% of butanediol, 0.3%-0.5% of asiaticoside-II, 0.5%-1.5% of centella asiatica, 0.5%-1.5% of vitamin E and the balance of water. And the balance of deionized water. By adding micro-crystalline cellulose (and) sphingomonas fermentation extract (and) cellulose gum, system stability under compounding of five organic acids is realized, semitransparent gelatinous texture is obtained, the composition is suitable for point coating use mode, impression is not affected, oil-controlling and acne-removing effects are improved under the condition of low organic acid content, and the composition belongs to skin-care polyacid products and has a broad market prospect. The mild oil control and acne removal effects are realized, and the cream is suitable for oil-sensitive skin crowds.

The invention discloses a film-forming agent composition as well as a preparation method and application of the film-forming agent composition. The film-forming agent composition is prepared from the following components in percentage by weight: 0.2 to 2 percent of hyaluronic acid and / or hyaluronate, 15 to 40 percent of polyhydric alcohol, 0.5 to 2 percent of gluconolactone and / or lactobionic acid lactone, and the balance of deionized water. The film-forming agent composition provided by the invention is obtained by adding a proper amount of polyol, gluconolactone and / or lactobionic acid lactone into an aqueous solution of active substance hyaluronic acid and / or hyaluronate, the polyhydric alcohol, gluconolactone and / or lactobionic acid lactone can form hydrogen bonds with the active substances hyaluronic acid and hyaluronate, and a complex hydrogen bond net can be formed by mixing the polyhydric alcohol, gluconolactone and / or lactobionic acid lactone with the active substances hyaluronic acid and hyaluronate, so that the flexibility of the film-forming agent composition can be greatly improved, the film-forming effect is good, the comprehensive performance of a formed film is excellent, and the film-forming agent composition can be widely applied to the field of film forming. The compound can be used as a film-forming agent to be applied to cosmetics and skin care products in the field of cosmetics and medicines in the field of medicines.

The invention discloses a pore cleaning mud mask and a preparation method thereof, belongs to the technical field of cosmetics, and particularly relates to a pore cleaning mud mask. Comprising 14 to 18 parts of kaolin, 7 to 9 parts of Manny root ice river mud, 4.5 to 5.5 parts of Amazon white mud, 10 to 12 parts of wheat germ oil, 43 to 47 parts of tea water, 0.1 to 0.14 part of a phellinus linteus extract, 1 to 2 parts of swertia mileensis powder, 2 to 4 parts of lactobionic acid, 0.08 to 0.12 part of salicylic acid and 0.4 to 0.6 part of gluconolactone. The pore cleaning mud mask provided by the invention is uniform in particle size distribution, uniform in mixing and capable of keeping the state of the mud mask moist and not dry in the use process, the cleaning strength of controlling oil, dredging pores and resisting inflammation is improved, meanwhile, skin sensitivity caused by excessive cleaning is avoided, and the cleaning effect is good. The invention provides a novel cleaning mud mask formula for realizing the goal of cleaning pores and an efficient and stable preparation method of the novel cleaning mud mask formula.

The invention belongs to the technical field of daily-use chemistry, and particularly relates to a keratin regulating composition containing mandelic acid and having skin smoothing and brightening effects and application of the keratin regulating composition. The composition provided by the invention comprises mandelic acid, lactobionic acid, hydroxyethyl piperazine ethanesulfonic acid, lactobacillus / soybean milk fermentation product filtrate and lactobacillus fermentation lysate. According to the application, mandelic acid, lactobionic acid, hydroxyethyl piperazine ethanesulfonic acid, lactobacillus / soybean milk fermentation product filtrate and lactobacillus fermentation lysate are compounded, so that the activity of KLK family proteases, especially kallikrein 5 (KLK5), can be obviously promoted, the decomposition of desmosome between keratinocytes is promoted, epidermal cells lose cohesion and finally naturally fall off, and the effect of promoting the activity of KLK family proteases is achieved. The cutin exfoliation promoting effect is achieved, so that the problems of skin darkness, roughness, pore blockage, acne and the like are solved, the glossiness and fineness of the skin are improved, and healthy and bright skin is achieved.

The invention relates to an allergy-relieving acne-removing composition and a preparation method and application thereof, and the allergy-relieving acne-removing composition comprises the following raw materials in parts by mass: 1-10 parts of VB-IP, 5-20 parts of hydroxyethyl urea, 1-5 parts of coated salicylic acid, 2-10 parts of wild soybean, 1-5 parts of clerodendranthus spicatus, 1-5 parts of rosewood seeds, 1-10 parts of lactobionic acid, 1-10 parts of gluconolactone, 1-10 parts of mandelic acid, 1-10 parts of glycollic acid, 1-5 parts of octyl dodecyl glucoside and 1-5 parts of cetearyl glucoside. 0.5 to 2 parts of phenoxyethanol and 5 to 30 parts of deionized water; the composition comprises the following components: a wild soybean fermentation extract, a clerodendranthus spicatus fermentation extract and a rosewood seed fermentation extract. Compared with the prior art, the formula is stable, penetration can be promoted, cutin renewal can be accelerated, a sebum film can be repaired, sebum can be adjusted, and the acne removing and skin care effects are good.

The invention relates to a halogen-free heavy oil removal surfactant and a preparation method thereof, and belongs to the technical field of surfactants. The heavy oil removal surfactant is prepared from fatty alcohol-polyoxyethylene ether, hydrophilic alkyl quaternary ammonium salt, a glycosyl nonionic surfactant and an alcohol compound, the glycosyl nonionic surfactant is prepared by sequentially carrying out hydrophobic amidation and hydrophobic epoxy addition on lactobionic acid lactone; the heavy oil removal surfactant is good in oil removal effect, safe, free of halogen and small in corrosivity, and the use performance is improved through compound use of the fatty alcohol-polyoxyethylene ether, the hydrophilic alkyl quaternary ammonium salt and the glycosyl nonionic surfactant.

The invention relates to a composition with exfoliating and whitening effects and application of the composition in cosmetics, and belongs to the technical field of cosmetics. The composition consists of the following components in parts by weight: 1-4 parts of lactobionic acid, 3-5 parts of a tea polysaccharide solution and 1-3 parts of kale leaf extract, the tea polysaccharide solution is prepared by fermenting bacillus subtilis, trametes hirsutum and tea, extracting and purifying. The content of tea polysaccharide in the tea polysaccharide solution is increased, the content of small molecular substances is increased, and permeation and absorption are facilitated. Meanwhile, the tea polysaccharide solution, lactobionic acid and kale leaf extract are combined for use, so that compared with other substances with similar effects, the combination effect is better, internal and external dual whitening and brightening can be realized, the effects are stably and lastingly played, the skin is not irritated, and the mask is fine and glossy.

The present invention discloses a fruit acid composition, a preparation method and an application thereof, belonging to the technical field of skin care products; the fruit acid composition provided by the present invention comprises a compound fruit acid, a compound soothing agent, betaine and D-panthenol; the compound fruit acid comprises lactic acid, glycolic acid, citric acid and lactobionic acid; the compound soothing agent comprises bisabolol and ginger root extract; by selecting appropriate fruit acids for compounding to form a compound fruit acid, and at the same time combining a compound soothing agent, betaine and D-panthenol, the obtained fruit acid composition can gently and non-irritatingly remove the cutin layer, whiten the skin and has excellent moisturizing effect on the skin. Further, when it is applied to the preparation of body wash, the obtained fruit acid body wash has excellent comprehensive performance.

The invention belongs to the technical field of tissue and organ preservation, and particularly relates to tissue and organ general preservation liquid. The general preserving fluid for tissues and organs is prepared from the following components: hydroxyethyl starch, sodium chloride, lactobionic acid, potassium phosphate, magnesium sulfate, bombax pentahydrate, adenosine, allopurine, glutathione, glycerol, heparin, fructose-1, 6-diphosphate, mannitol, an antibacterial component, insulin, simethicone and the balance of distilled water. The general preserving fluid for tissues and organs can preserve the overall physiological activity of the tissues and organs for a long time in a non-frozen state or a frozen state.

The invention discloses a preparation method of zero-lactose yoghurt fermented milk for infants, which specifically comprises the following steps: firstly, performing high-temperature sterilization on raw milk, then cooling, and adding lactase Nola Fit 5500 before fermentation to perform pre-hydrolysis of lactose so as to ensure zero lactose of the product from the source. Then, compound probiotics composed of bifidobacterium animalis subsp. Lactis HN019, lactobacillus mucus fermentation CECT5716, lactobacillus rhamnosus HN001 and bifidobacterium breve M-16V are added, low-temperature slow fermentation is carried out for 15 hours at the temperature of 41 + / -1 DEG C, and no exogenous sugar is added in the whole process. According to the process, nutritional ingredients are reserved to the maximum extent through mild fermentation, formation of natural flavor is promoted, and the obtained product is safe, nutritional and easy to digest and can effectively improve intestinal health of infants and reduce the risk of digestion discomfort.

The present invention relates to the field of cosmetic technology, and particularly, to a whitening and anti-aging composition, a method for preparing same, and use thereof. The whitening and anti-aging composition is prepared from the following ingredients in parts by weight: 0.1-2 parts of hydroxypropyl cyclodextrin; 0.1-2 parts of tocopherol; 1-5 parts of an emulsifier; 0.5-1 part of a preservative; 1-5 parts of a functional solvent; 0.1-0.9 part of retinol; 0.1-3 parts of an adjusting mixed acid agent; and 90-100 parts of deionized water. The adjusting mixed acid agent consists of lactobionic acid, inositol hexaphosphate, and ferulic acid, and the ratio of lactobionic acid to inositol hexaphosphate to ferulic acid is (5-7):(0.5-1):1. The method for preparing the whitening and anti-aging composition comprises: dissolving the adjusting mixed acid agent in a portion of deionized water; adding hydroxypropyl cyclodextrin, the emulsifier, and retinol into the remaining deionized water and heating for dissolution; and finally, mixing and stirring the two resulting solutions along with the addition of tocopherol and the preservative during the process to give the composition. The composition exhibits excellent whitening and anti-aging effects with low irritation, and is also suitable for populations having sensitive skin with damaged skin barriers.

The application belongs to the technical field of cosmetics, and discloses a Rosa chinensis / Rhizopus stolonifer fermenting composition and a moisturizing and oil-controlling composition. The preparation method of the Rosa chinensis / Rhizopus stolonifer fermenting composition is as follows: dried Rosa chinensis petals are ground and sieved, inoculated into a fermentation medium, and then inoculated with Lactobacillus pentosus for fermentation culture; the obtained fermentation liquor is inactivated, filtered, concentrated and purified to obtain a Rosa chinensis fermentation product filtrate; then the Rosa chinensis fermentation product filtrate is mixed with a Rhizopus stolonifer fermentation product filtrate to obtain the Rosa chinensis / Rhizopus stolonifer fermenting composition. The moisturizing and oil-controlling composition comprises Hamamelis virginiana extract, Buxus seed extract, lactobionic acid, tea tree essential oil, the Rosa chinensis / Rhizopus stolonifer fermenting composition, supermolecular inclusion suberic acid, a surfactant and water. The composition has the synergistic effects of moisturizing, oil-controlling and skin tightening.

The invention discloses a method for preparing calcium threonate by using calcium galactobionate as a raw material and application of the calcium threonate. The method comprises the following steps: S1, adding the calcium galactobionate into hydrochloric acid to generate galactobionic acid and calcium chloride; s2, under the protection of nitrogen, soluble ferrite is added into the mixed solution obtained in the S2, H2O2 is dropwise added, Fe < 2 + > catalyzes H2O2 based on the Fenton reaction principle to generate hydroxyl free radicals, galactobionic acid C4-C5 sites are directionally attacked to break, and erythritol is generated; s3, adjusting the pH value of the reaction system, and separating Fe (OH) 3 colloidal particles; s4, adjusting the pH value of the erythritol dispersion liquid to 9-10, and performing alkali-induced ring opening and epimerization on the erythritol to obtain calcium ions in the threonic acid complexing solution; s5, crystallizing and purifying the calcium threonate obtained in S4 to obtain a target product. The calcium threonate obtained by the invention is applied to a calcium supplement; the method is high in catalytic selectivity, low in byproduct content and convenient to separate and purify.

The invention discloses a synthetic method of lactobionic acid. The synthetic method comprises the following steps: S1, adding tetrabutylammonium bromide and TEMPO / NaBr / NaClO into a lactose aqueous solution with the mass fraction of 10-15%, and carrying out catalytic oxidation to obtain lactobionic acid; s2, adding sodium phytate; s3, cooling the filtrate obtained in S2, dropwise adding oxalic acid until the pH value of the system is 2.0-2.5, standing for layering, washing a lower heavy phase containing TEMPOH + Cl <-> precipitate with pure water, and combining the filtrate; s4, passing through an activated carbon column, and collecting a decoloring solution; s5, concentrating and cooling the decoloring solution, injecting liquid CO2, and maintaining the pressure; then, acetone is added in a gradient mode for three times, and alpha-lactobionic acid crystals are obtained; according to the method, tetrabutylammonium bromide is used for transferring a hydrophobic main catalyst TEMPO < + > from an organic phase to a water phase through an ion pair exchange mechanism, so that the oxidation reaction of C6-site hydroxyl of lactose is accelerated, and the reaction efficiency is improved while the consumption of NaBr is reduced.

The present invention relates to a method for preparing a formulation the active principle of which is oligogalacturonide compounds of linear chains of α-(1,4)-D-galacturonic acid residues with a degr

This invention relates to the field of cosmetic technology, specifically disclosing a synergistic exfoliating composition and a method for preparing a controlled-release carrier containing the composition. The synergistic exfoliating composition is a compound of adenosine, N-acetylneuraminic acid, and lactobionic acid. This invention has the advantage of maintaining good exfoliating efficacy while reducing skin irritation.

The invention discloses micro-molecular metastructured 4-butylresorcinol and a preparation method and application thereof, a skin external preparation and cosmetics, and belongs to the technical field of cosmetics. According to the technical scheme, the micro-molecular metastructured 4-butylresorcinol is obtained through hydrogen bond interaction of lactobionic acid and 4-butylresorcinol in water; according to the supramolecular complex provided by the invention, the lactobionic acid and the 4-butylresorcinol form the supramolecular complex, on one hand, both the lactobionic acid and the 4-butylresorcinol have certain whitening and antioxidant capacities, and on the other hand, due to a supramolecular complex structure formed by the lactobionic acid and the 4-butylresorcinol, the water solubility of the 4-butylresorcinol can be improved; hydroxyl on 4-butylresorcinol can be prevented from being oxidized, so that the 4-butylresorcinol can keep good oxidation resistance.

Cosmetic agents may be used for treating a keratin material. The cosmetic agent may include lactobionic acid in an amount ranging from 0.001 to 20 wt. % in relation to the total weight of the cosmetic agent, and the cosmetic agent may further include at least one surfactant in an amount ranging from 0.01 to 40 wt. % in relation to the total weight of the cosmetic agent.

A biphasic product comprising lactobionic acid, lactic acid or corresponding salts thereof and / or gluconolactone.

The invention relates to the technical field of cosmetics, in particular to a blue copper peptide-containing composition and application thereof in cosmetics. The composition comprises the following components in parts by mass: 1 part of blue copper peptide; 0.5 to 5 parts of tetrahydromethylpyrimidine carboxylic acid; 0.05 to 0.5 part of sodium hyaluronate; and 0.05 to 0.5 part of beta-glucan. Preferably, the composition further comprises allantoin and lactobionic acid, based on 1 part by mass of the blue copper peptide, the content of the allantoin is 0.1-0.5 part, the content of the lactobionic acid is 0.2-1.0 part, and the mass ratio of the allantoin to the lactobionic acid is 1: (2-4). In-vitro efficacy experiments prove that the specific combination and proportion can generate a remarkable synergistic effect, and meanwhile, the capabilities of inhibiting the activity of elastinase and promoting epidermal cell repair are improved, so that the excellent dual effects of wrinkle resistance and skin barrier repair are realized. The composition can be used for preparing cosmetics such as essence, emulsion and face cream.

The present application discloses a method for detecting the purity of lactobionic acid in the preparation process of erythromycin lactobionate, comprising the following steps: establishing a linear regression equation between conductivity and the concentration of sodium lactobionate solution, and deriving the relationship between the theoretical concentration of lactobionic acid and conductivity; detecting the actual concentration of lactobionic acid using titration; measuring the conductivity of the solution after titration, and calculating the theoretical concentration of lactobionic acid according to the relationship; the ratio of the actual concentration to the theoretical concentration is the purity. This detection method can detect the purity of lactobionic acid after cation exchange in real time, ensuring that its value is above the standard, which is conducive to the preparation of erythromycin lactobionate with stable quality and ensuring the safety of patients' medication. The above detection method is simple and easy to control, low cost and strong stability.

The invention discloses a pH self-adaptive private care lotion based on a composite biological antibacterial system. The pH self-adaptive private care lotion is prepared from 8 to 12 parts of cocamidopropyl betaine, 0.5 to 1.5 parts of polylysine hydrochloride, 0.3 to 0.8 part of tea tree essential oil, 1.2 to 2.0 parts of lactic acid-sodium lactate buffer agent, 0.1 to 0.3 part of dipotassium glycyrrhizinate, 0.5 to 1.2 parts of chamomile extract, 0.3 to 0.7 part of lactobionic acid, 1.0 to 2.0 parts of aloe polysaccharide and the balance of deionized water. During preparation, the tea tree essential oil and beta-cyclodextrin are subjected to clathration for 30 minutes at 35 DEG C to form a clathrate compound, so that the volatile loss is reduced by 80%; dissolving polylysine hydrochloride in deionized water at 4 DEG C, and adding a lactic acid-sodium lactate buffer solution; lactobionic acid and a chamomile extract are added; homogenizing for 3 minutes by using a homogenizer at the speed of 2000rpm; and carrying out vacuum defoaming under 0.08 MPa. Dynamic pH buffering is achieved, and a lactic acid-lactobionic acid double-buffering layer resists secreta impact; a preservative-free process is adopted, and vacuum defoaming and PES membrane filtration are used for replacing chemical preservatives; and gender differentiation adaptation: cranberry polyphenol, female adhesion prevention and succinic acid buffer male pH adaptation.

Methods of extending a period of increased soil nitrogen levels in a soil are described. The methods include applying a soil enhancement agent to the soil, where the soil enhancement agent may include one or both of (i) lactobionic acid, and (ii) a least one salt of lactobionic acid. The methods also include applying a nitrogen-containing fertilizer to the soil. The soil treated with both the soil enhancement agent and the nitrogen-containing fertilizer has a higher level of soil nitrogen for at least a portion of a grown cycle of a crop planted in the soil than the same soil treated without the soil enhancement agent during the growing cycle of the crop.

The invention discloses a polyurethane adhesive and a preparation method thereof, and relates to the technical field of adhesives, the polyurethane adhesive is prepared from the following components by weight: 100 parts of waterborne hyperbranched polyurethane containing active end groups, 10 parts of 1, 3, 4-trimethyl-1, 3, 4-trimethyl-1, 3, 4-trimethyl-1, 3, 4-trimethyl-1, 3, 4-trimethyl-1, the waterborne polyurethane adhesive is prepared from the following components in parts by weight: 3 to 5 parts of 1, 3, 5-tris (2-hydroxyethyl) cyanuric acid, 4 to 6 parts of lactobionic acid, 5 to 10 parts of chain extender, 3 to 5 parts of cross-linking agent, 20 to 30 parts of isocyanate, 5 to 10 parts of polyol, 0.3 to 0.5 part of catalyst, 0.8 to 1.2 parts of neutralizer, 60 to 80 parts of water and 0.1 to 0.3 part of waterborne defoaming agent. The adhesive is high in bonding strength, good in weather resistance, sufficient in water resistance and excellent in flame retardance and environment friendliness.

The invention relates to a ready-to-use serum-free DMSO-free cell cryopreservation solution, a preparation method and application, and relates to the field of cell cryopreservation, and the cell cryopreservation solution comprises the following components: sodium lactobionate, calcium chloride, magnesium chloride, potassium dihydrogen phosphate, potassium bicarbonate, potassium chloride, sucrose, mannose, trehalose, hydroxyethyl starch, 4-hydroxyethyl piperazine ethanesulfonic acid, water-soluble vitamin E and ethylene glycol. The cell cryopreservation liquid provided by the invention has the advantages of high efficiency, no foreign protein, easiness in use and the like, cells resuscitated after cryopreservation are high in activity, and the cell characteristics can be kept after resuscitation; the cell freezing medium does not contain serum and other foreign proteins, so that the influence of endotoxin, latent virus and inter-batch difference is avoided; the cell freezing medium disclosed by the invention does not contain DMSO, so that the toxic effect on cells is further reduced, and the cell characteristics are favorably maintained; the cell freezing medium can be directly stored in a refrigerator at the temperature of-80 DEG C without programmed cooling, and is quick and convenient to use.