49 results about "Hepatic Cancers" patented technology

This invention relates to the field of medical device technology, specifically to an auxiliary device for hepatic artery angiography in interventional treatment of liver cancer. The device includes a fixed carrier with a flexible base and several supporting components. Each supporting component includes several arc-shaped connecting strips hinged together. Several adjustment boxes are installed on both sides of the flexible base, each containing an adjustment component. Each adjustment component includes a drive element with a bidirectional lead screw fixed to it. Nut seats are threaded to both ends of the bidirectional lead screw, and sliders are fixed to the nut seats. Traction cables are fixedly connected to the sliders, and the traction cables are hinged to the nodes of the arc-shaped connecting strips. The drive element is signal-connected to a control module. The control module controls the drive element to rotate the bidirectional lead screw forward and backward according to preset patient body shape parameters. This causes the sliders to slide through the nut seats, thereby causing the traction cables to pull the nodes of the arc-shaped connecting strips. This invention achieves multi-dimensional safety protection through mechanical structure linkage and sensor feedback, reducing surgical field interference.

Abstract The present invention provides antibodies useful as therapeutics for treating and / or preventing diseases associated with cells expressing CLD18, including tumor-related diseases such as gastric cancer, esophageal cancer, pancreatic cancer, lung cancer, ovarian cancer, hepatic cancer, head-neck cancer, and cancer of the gallbladder. 2020220128 20 Aug 2020

The invention discloses a mouse liver cancer cell line with a high lung metastasis characteristic as well as a construction method and application thereof, and belongs to the technical field of biology and oncology. The cell is preserved in China Center for Type Culture Collection on February 4, 2026, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: C202621. The PNV-LM3 cell line provided by the invention is constructed by combining a PTEN KO and NRAS G12V mutated hydrodynamic tail vein injection model with tail vein injection in-vivo domestication, a mouse liver cancer model is successfully induced to generate lung metastasis, and the lung metastasis tendency of the cell line is greatly enhanced. Compared with a traditional Hepa1-6 cell line, the PNV-LM3 cell line has the advantages that the lung metastasis process of the liver cancer is more accurately and efficiently reproduced, and a more real experimental platform is provided for research of a liver cancer metastasis mechanism.

This invention discloses a sophoridine tricyclic derivative or its pharmaceutical salt, with the following general structural formula: R1 is selected from hydrogen, C1-C20 straight-chain alkyl, and C1-C20 branched alkyl. In vitro experiments have verified that the sophoridine tricyclic derivative can inhibit TGF-β-induced activation of hepatic stellate cells, improve CCl4- and bile duct ligation-induced liver injury and liver fibrosis in mice, inhibit TGF-β / Smads signaling pathway activation, and also inhibit diethylnitrosamine-induced primary liver cancer in mice. Therefore, this invention provides new evidence for the treatment, alleviation, or improvement of liver fibrosis or primary liver cancer using sophoridine tricyclic derivatives.

The invention relates to the technical field of medicines, in particular to an enantiomer-atesane type diterpenoid compound as well as a preparation method and an application of the enantiomer-atesane type diterpenoid compound. The enantiomer-atesane diterpenoid compound disclosed by the invention is derived from plants, has anti-tumor activity, has cytotoxicity to human leukemia cells, human liver cancer cells and human cervical cancer cells, and is relatively good in inhibitory activity. Wherein the compound 3 has the best effect, the IC50 values of the compound 3 to three human tumor cells are 7.5 + / -1.2 micromole per liter, 9.8 + / -1.0 micromole per liter and 10.1 + / -0.9 micromole per liter respectively, and the activity of the compound 3 is equivalent to that of a positive control drug mitomycin. Particularly, the compound 3 also has good cytotoxicity to drug-resistant liver cancer cells (Huh7-LR cells), and the IC50 value of the compound 3 is 9.6 + / -1.1 micromole per liter. The compound 3 shows huge potential as a lead compound by virtue of the novel chemical structure and potent cytotoxicity shown in various cell lines.

The application belongs to the technical field of biological medicine, and particularly relates to a galactosamine-doxetaxel conjugate as well as a preparation method and application thereof. First, doxetaxel and 3,3'-diseleno-dipropionic acid are used as raw materials, and after heating reaction, 3,3'-diseleno-dipropionic acid doxetaxel is obtained. Then, the obtained 3,3'-diseleno-dipropionic acid doxetaxel and galactosamine are used as raw materials, and after heating reaction, galactosamine-3,3'-diseleno-dipropionic acid doxetaxel conjugate is obtained through column chromatography separation and purification. The obtained conjugate can be specifically recognized by endogenous lectin receptors (such as asialoglycoprotein receptor ASGPR) which are highly expressed on the surface of hepatoma cells, so as to be taken up by hepatoma cells through a receptor-mediated pathway, and has application prospect in the direction of hepatoma targeted treatment.

The application belongs to the technical fields of synthesis of compounds and pharmaceutical applications, and discloses an anti-tumor pterostilbene piperazine conjugate as well as a preparation method and application thereof. The pterostilbene, piperazine and various substituents are spliced in one molecule by using a molecular hybridization method, a novel anti-tumor pterostilbene piperazine conjugate is synthesized, and it is found through anti-tumor activity research that the anti-tumor activity of the conjugate is obviously higher than that of the two pharmacophores alone, and the conjugate has the potential to become a novel anti-tumor drug. The pterostilbene piperazine conjugate is synthesized, and it is found for the first time that the compound has good anti-tumor activity, can significantly inhibit the proliferation of various tumor cells in vitro, including hepatocarcinoma, triple-negative breast cancer, colon cancer and ovarian cancer cells, has little toxicity to normal cells, and can inhibit the migration ability of tumor cells. Therefore, the compound synthesized by the application has good anti-tumor application prospect.

This application relates to an evaluation method and system in the quality control process of liver cancer. The method includes decomposing a set of received three-dimensional ultrasound contrast images according to connection points; grouping objects belonging to different three-dimensional ultrasound contrast images into the same comparison group, with objects in each comparison group generated based on the same structural tissue; sorting multiple objects in a comparison group according to generation time; comparing the degree of change of two adjacent objects belonging to the same comparison group in the time series, and statistically analyzing the number of objects whose degree of change exceeds the allowable degree of change, or calculating the ratio of the sum of the lengths of the parts of the objects whose degree of change exceeds the allowable degree of change to the sum of the lengths of all objects. This application provides an evaluation method and system in the quality control process of liver cancer, which achieves quantitative analysis of local images and provides a comparison of changes in treatment effects before and after treatment by using spatial decomposition and degree of change comparison, thereby providing data reference for subsequent liver cancer quality control processes.

This invention discloses a mixed hepatocyte terahertz quantitative detection system, electronic device, and medium, comprising: a spectral preprocessing module for acquiring a mixed hepatocyte time-domain spectrum and converting it into a mixed hepatocyte terahertz frequency-domain spectrum using a fast Fourier transform; calculating the refractive index and absorption coefficient to obtain frequency-domain signals of the refractive index and absorption coefficient, and processing them using Gram angle sum field and Gram angle difference field to obtain Gram angle sum field map and Gram angle difference field map; an image feature extraction module for extracting gradient features from the Gram angle sum field map and Gram angle difference field map using gradient histograms; extracting grayscale features from the Gram angle sum field map and Gram angle difference field map using grayscale histograms; fusing the gradient features and grayscale features to obtain image features; and a hepatocyte cancer cell content quantitative detection module for inputting the image features into a pre-trained hepatocyte cancer cell content quantitative detection model to obtain the proportion of hepatocyte cancer cells.

The application provides a method and device for recommending a hepatic cancer embolism placement point planning, wherein the method comprises the following steps: obtaining input data; segmenting the input data to obtain a segmentation result of the input data, and classifying the segmentation result to determine whether there is a high-risk tumor in the liver; if there is a high-risk tumor in the liver, calculating an embolism placement point according to the segmentation result, generating an embolism placement point planning result; synthesizing the embolism placement point planning result with an original medical image to generate a recommended scheme, and outputting the recommended scheme. Through deep learning, machine learning and traditional image processing methods, various organs such as patient arterial vessels, livers, tumors and bones are automatically segmented, and according to the automatically generated segmentation information, recommended information is generated to recommend the embolism placement point of each tumor, so as to assist doctors in comprehensively and deeply understanding the condition of the patient.

The application discloses a stable liver cancer embolism emulsion of raltitrexed drug gel and a preparation method and application thereof, and belongs to the field of pharmaceutical preparations. The emulsion is composed of iodized oil embolism agent and raltitrexed drug gel; the raltitrexed drug gel is a nanofiber network hydrogel formed by self-assembly of raltitrexed molecules in an aqueous phase through ultrasonic treatment, and simultaneously serves as an active pharmaceutical ingredient and an emulsion stabilizer. The iodized oil and the raltitrexed drug gel are physically mixed and injected through a three-way valve to form a stable oil-in-water emulsion or a water-in-oil emulsion, and any additional chemical surfactant or cosolvent is not needed. The emulsion has excellent physical stability, and can effectively solve the problems of easy aggregation and sedimentation of a traditional iodized oil emulsion and increased systemic toxicity caused by drug burst release. The emulsion has good embolism effect and drug release behavior, can significantly inhibit tumor growth, and has a wide clinical transformation prospect.

The invention discloses a construction method of an in-vitro liver cancer liver invasion and metastasis experimental model, and relates to the technical field of model construction, and the construction method comprises the following steps: culturing liver cancer cells and hepatic stellate cells, carrying out fluorescence labeling, generating a space coordinate set by using the fluorescence labeled liver cancer cells and hepatic stellate cells, and calculating the in-vitro liver cancer liver invasion and metastasis experimental model. The method comprises the following steps of: printing liver cancer cells and hepatic stellate cells according to a space coordinate set to generate a three-dimensional cell-matrix structure, pouring photoresponse hydrogel into the three-dimensional cell-matrix structure, and inducing HOTAIR long non-coding RNA concentration by using a transforming growth factor to obtain the three-dimensional cell-matrix structure integrated with the photoresponse hydrogel. The space coordinate set is generated through the Markov model, accurate simulation of radial distribution of the highly invasive liver cancer cell core area and the hepatic stellate cells is achieved, and an accurate position template is provided for printing of a three-dimensional cell-matrix structure.

The application discloses an alpha-linolenic acid and metal iridium coupling compound, a preparation method and application thereof, and relates to the field of medicine, in particular to an alpha-linolenic acid and metal iridium coupling compound, which has good antitumor activity on human cervical cancer cells, human hepatoma cells and mouse breast cancer cells. The preparation method is simple and easy to operate, and the product has good antitumor activity.

The invention relates to a compound and application thereof in treatment of chronic hepatitis B, hepatic fibrosis and liver cancer. Specifically provided are a compound represented by formula I, or a stereoisomer, a prodrug, a pharmaceutically acceptable salt or a pharmaceutically acceptable solvate of the compound,

The invention discloses a preparation method and application of a urea triazole derivative which is simple and easy to operate, cheap and easily available in raw materials, high in reaction efficiency and good in inhibition effect on human liver cancer cells Huh-7, and belongs to the technical field of medicine synthesis. According to the key points of the technical scheme, the urea triazole derivative molecule has a structure, or R1 is hydrogen atom, fluorine, chlorine, bromine, iodine, nitryl, alkyl (such as methyl, ethyl or alkynyl) or alkoxy (such as methoxyl) and various substituent groups, R2 is phenyl derivative or benzyl derivative, and X is oxygen atom or sulfur atom. The invention designs and synthesizes a urea triazole derivative with a novel structure, and the compound has a good inhibition effect on human liver cancer cells Huh-7.

The application belongs to the technical field of medicine, and relates to extraction and separation of a guaiane sesquiterpene glycoside compound guaiane sesquiterpene glycoside II from rhizome of rabdosia japonica by using macroporous resin, normal-phase silica gel, reverse-phase silica gel and dextran gel column chromatography and recrystallization and the like. 21 H 36 O8, in-vitro anti-tumor activity research shows that the compound has good anti-tumor activity, including obvious inhibition on human gastric cancer cells BGC-823, human hepatoma cells HepG-2 and human lung cancer cells A549. The application can provide a source of pharmacodynamic material basis for development of anti-tumor drugs, and has development and application prospects.

The application discloses a kind of nuclear magnetic resonance image prediction model construction methods, main design concept is in, from the information between using multi-stage liver cancer nuclear magnetic image data start to improve the prediction performance of the prediction model constructed, specifically, propose by multiple multilayer convolutional neural network as the extraction part of image feature, in this link, introduce multi-sequence image difference extraction pyramid module, difference information is mined between the input two two image stages and is integrated into image feature extraction operation, then the feature map containing difference information extracted by convolutional neural network is sent into multi-sequence image correlation attention module, learn the correlation between the input different stage nuclear magnetic image, and then provide rich reference information for subsequent accurate prediction classification.The present application can make full use of the difference information and correlation information between the images of different stages in the enhanced nuclear magnetic image, so as to effectively improve the prediction performance of the model based on image information.

The invention belongs to the technical field of polypeptide drugs, and particularly relates to a series of polypeptides with an anti-cancer effect and application thereof. According to the invention, Rink amide MBHA amino resin is used as a solid phase carrier, modification is carried out according to an amino acid sequence of a template polypeptide Hymenochirin-1Pa (H-0): Ac-LKLSPKTKDTLKKVLKGAIKGAIAIASAMA-NH2, and on the basis of retaining key amino acid residues, original amino acids are replaced by R8 and S5 at the positions of i and i + 7 amino acids, so that the target stapling peptide is obtained. Compared with a template polypeptide H-0, the obtained stapled peptide has the advantage that the anti-tumor activity on human liver cancer cells Huh7, human non-small cell lung cancer cells A549, glioma cells U87 and colon cancer cells T84 can be obviously improved.

The invention discloses an analysis and identification method of N-acylamino acid and application of the analysis and identification method. According to the method, a one-pot method is adopted, amino acid and fatty acid are synthesized into a target object under the action of a coupling reagent, a derivatization reagent N, N-diisopropylethylenediamine (DIAAA) is directly added for in-situ chemical derivatization without separation, and then liquid chromatography-mass spectrometry (LC-MS) analysis is carried out. And carrying out structure identification according to the DIAAA characteristic fragment, the amino acid residue fragment and the acyl fragment. The method can be extensively used for systematic identification and quantitative analysis of complex biological samples, and is especially suitable for N-oleoyl amino acid. Experiments prove that N-oleoyl tyrosine, proline, leucine and tryptophan have remarkable inhibitory activity on hepatoma cells HepG2, which indicates that the N-oleoyl tyrosine, proline, leucine and tryptophan have application potential in preparation of anti-hepatoma drugs. According to the method, efficient and accurate analysis of the N-acylamino acid is realized.

The invention discloses an NK cell killing resistant cell strain as well as a construction method and application thereof. The NK cell killing resistant cell strain is named as a human liver cancer cell HepG2-NK-R Homo sapiens, and is preserved in the China Center for Type Culture Collection on November 05, 2025, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: C2025298. The human hepatoma cell line HepG2 is exposed to NK cells for a long time for co-culture to obtain the human hepatoma cell line HepG2. The cell strain has a stable biological phenotype through in-vitro simulation of NK cell mediated long-term immune editing. Compared with parental cells, the cell line shows remarkably enhanced malignant biological behaviors, can be used for researching occurrence, development and metastasis of liver cancer or preparing a tumor cell model or a tumor animal model, is greatly improved in tumor forming ability, in-vivo proliferation speed and metastasis speed, can remarkably shorten an experimental period, and can highly restore invasion characteristics of tumors after evolution.

The invention discloses siRNA (small interfering Ribonucleic Acid) for inhibiting the expression of a chicken RASGRP3 (Reduced Amplification of Sequence Growth Protein 3) gene and application of the siRNA, the siRNA comprises a positive-sense strand and an antisense strand, and is any one of RASGRP3-Anas-214, RASGRP3-Anas-846 and RASGRP3-Anas-1187. According to the specific siRNA aiming at the chicken RASGRP3 mRNA provided by the invention, in a chicken liver cancer cell line (LMH), after liposome-mediated transfection culture is performed for 48 hours, RT-qPCR is adopted to evaluate the transcript level, and a result shows that the relative expression quantity of the chicken RASGRP3 mRNA is remarkably reduced compared with that of negative control, and the repeatability is good. The polypeptide can be further used for inhibiting ALV-J replication, provides a new molecular intervention target for prevention and control of avian leukosis, and has important scientific research and potential economic values.

The invention belongs to the technical field of medicines, and particularly relates to a substituted 2-(piperazine-1-yl) acetamide derivative as well as a preparation method and application thereof. The structure of the compound is shown as a formula I. Experiments prove that the compound has good anti-hepatoma activity and lays a foundation for preparation of anti-hepatoma drugs. Formula I

The invention belongs to the technical field of cellular immunotherapy, and particularly relates to a targeted FAP chimeric antigen receptor macrophage as well as a preparation method and application thereof. According to the invention, FAP is taken as a target spot to design a CAR carrier of a specific targeted activated hepatic stellate cell, and a CAR-M cell for treating hepatic fibrosis is constructed. The macrophage expressing the chimeric antigen receptor has a remarkable killing effect on FAP positive cells, and a new thought is provided for clinical treatment of hepatic fibrosis, liver cirrhosis and liver cancer.

This invention belongs to the fields of medicinal chemistry and organic synthesis, specifically relating to a stable 3-aminocyclobutane-1,1-dicarboxylic acid diethyl oxalate and its application in the preparation of 3-(cholate-valerate)cyclobutane-1,1-dicarboxylic acid. This invention effectively solves the technical bottlenecks of poor stability, difficulty in purification, and storage by converting unstable free 3-aminocyclobutane-1,1-dicarboxylic acid diethyl oxalate into a specific organic acid salt form. More importantly, this invention discovers that this organic acid salt can be directly used as a reaction intermediate, and can be used efficiently in subsequent amide condensation reactions with cholic acid or its active derivatives without the separation of free amines. This method significantly improves the purity and yield of the key intermediate and its downstream product 3-(cholate-valerate)cyclobutane-1,1-dicarboxylic acid. The process is stable and simple to operate, providing a stable, controllable, and economical preparation route for the industrial production of 3-(cholate-valerate)cyclobutane-1,1-dicarboxylic acid, a key active pharmaceutical ingredient in the liver cancer prodrug LLC-202.

The invention discloses a liver cancer ablation operation evaluation method, device, equipment and medium, and relates to the technical field of medical assistance, the method comprises the following steps: establishing a mechanical correlation between a temperature field and a deformation field through thermoelastic coupling learning, predicting an ablation area, calculating an ablation boundary based on a three-dimensional space relation, and mapping the ablation boundary to a two-dimensional plane; and finally, a curative effect evaluation result is visually presented in a color grading manner. The spatial orientation information of the directional ablation boundary evaluation index is visually associated with the ablation safety boundary through Movit projection, and decision support for rapidly identifying an insufficient ablation area and planning supplementary treatment is provided for clinic.

The invention discloses a cabozantinib liver-targeting lipid nanoparticle injection as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations. The cabozantinib injection is prepared from the following components in percentage by mass: 0.8 to 1.5 percent of cabozantinib, 2.5 to 4.0 percent of DSPE-PEG2000-galactose, 35.0 to 42.0 percent of dipalmitoyl phosphatidylcholine, 6.0 to 8.0 percent of sitosterol, 3.0 to 5.0 percent of composite freeze-drying protective additive and the balance of citric acid-sodium citrate buffer solution with the pH value of 4.0, and the composite freeze-drying protective additive is a mixture of hydroxypropyl-cyclodextrin and mannitol in a mass ratio of 2 to 1. The preparation provided by the invention reduces systemic toxicity, significantly improves the drug concentration of liver and tumor parts, has better tumor inhibition effect than clinical positive drug adriamycin, is suitable for treatment of liver cancer and hepatic metastatic tumor, and has a relatively high application prospect.

The application provides a cell line based on Spacer gene directional insertion of HiBit tags, a recombinant virus and a preparation method and application thereof, linearized fragments are obtained by double enzyme digestion of a vector pCDN3.1-CMV-G418 through SacI / PmeI, fragments 1 and 2 are obtained by amplifying a D-type HBV 1.3-fold plasmid through primers shown as SEQ ID NO. 4-SEQ ID NO. 5 and primers shown as SEQ ID NO. 6-SEQ ID NO. 7, and the linearized fragments and the recombinant plasmid are obtained through seamless cloning, the recombinant plasmid is transfected into hepatoma cells after single enzyme digestion through ScaI, and a cell line with replication ability based on Spacer gene directional insertion of HiBit tags is obtained through resistance screening. A novel recombinant virus with replication and infection ability can be obtained from the cell model, the whole life cycle of the virus can be completed, simple and rapid detection and high-throughput screening of antiviral drugs can be realized.

The invention relates to the technical field of medical image processing, and discloses a liver cancer image automatic segmentation identification method fusing a multi-modal image. The method comprises the following steps: acquiring multi-modal medical image data in a first processing stage, and judging whether the data contains artifact interference characteristics or not; if artifact interference features are included, a first processing flow is triggered, time sequence registration processing is carried out on the data, a registered first image feature set is obtained in a second processing stage, and a first tumor boundary segmentation result is generated based on a first segmentation network model and the first image feature set; and if the artifact interference feature is not included, triggering a second processing flow, performing feature enhancement processing on the data, obtaining an enhanced second image feature set in a third processing stage, and generating a second tumor boundary segmentation result based on the second segmentation network model and the second image feature set. According to the method, multi-modal image data with different qualities can be processed in a targeted manner, the segmentation adaptability and reliability are improved, and clinical application requirements are met.

The application provides a 4-substituted phenylacetamide compound and a preparation method and application thereof, and a structural formula of the compound is shown as formula (I) or (II). The compound provided by the application is a kind of sphingosine kinase 2 (SphK2) targeted small molecule, can effectively inhibit SphK2 enzyme activity and human hepatoma (HepG2), murine hepatoma (Hepa1-6), human colon cancer (HCT116), murine colon cancer (MC-38), cervical cancer (MCF-7), gastric cancer (HGC-27) and other cancer cell proliferation and migration, and the inhibiting effect is better than that of a known inhibitor ABC294640, has the potential to be developed into a new anticancer drug. The synthesis steps of the compound are simple, the requirement for equipment is low, and the operation is simple, so that the yield of the reaction can be greatly improved, and the production cost is further saved.

The application provides a use of Tibetan tea or an extract thereof in preparation of a chemotherapy drug sensitizer, and belongs to the field of medicines. The application first finds that the water extract of Tibetan tea can significantly down-regulate the expression of a multi-drug resistance transporter P-gp in human hepatoma HepG2 cells, inhibit the proliferation of the human hepatoma HepG2 cells, reduce the drug resistance of the HepG2 cells, and enhance the sensitivity of the hepatoma cells to a chemotherapy drug paclitaxel. The application also first finds that the water extract of Yaan Tibetan tea and the paclitaxel play a synergistic effect in inhibiting the expression of the multi-drug resistance transporter P-gp in the human hepatoma HepG2 cells. The water extract of the Tibetan tea combined with the paclitaxel is used for treating hepatoma, and has a good application prospect.