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9600 results about "Image manipulation" patented technology

Reagents, methods, and libraries for bead-based sequencing

The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. In certain embodiments the methods make use of extension probes containing an abasic residue or a damaged base and employ agents appropriate to cleave linkages between a nucleoside and an abasic residue and/or agents appropriate to remove a damaged base from a nucleic acid. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to beads, which are immobilized in or on a semi-solid support. The invention further provides sets of labeled extension probes containing phosphorothiolate linkages or trigger residues that are suitable for use in the method. In addition, the invention includes performing multiple sequencing reactions on a single template by removing initializing oligonucleotides and extended strands and performing subsequent reactions using different initializing oligonucleotides. The invention further provides efficient methods for preparing templates, particularly for performing sequencing multiple different templates in parallel. The invention also provides methods for performing ligation and cleavage. The invention also provides new libraries of nucleic acid fragments containing paired tags, and methods of preparing microparticles having multiple different templates (e.g., containing paired tags) attached thereto and of sequencing the templates individually. The invention also provides automated sequencing systems, flow cells, image processing methods, and computer-readable media that store computer-executable instructions (e.g., to perform the image-processing methods) and/or sequence information. In certain embodiments the sequence information is stored in a database.
Owner:APPL BIOSYSTEMS INC

Human-machine interaction method and device based on sight tracing and gesture discriminating

The invention discloses a human-computer interaction method and a device based on vision follow-up and gesture identification. The method comprises the following steps of: facial area detection, hand area detection, eye location, fingertip location, screen location and gesture identification. A straight line is determined between an eye and a fingertip; the position where the straight line intersects with the screen is transformed into the logic coordinate of the mouse on the screen, and simultaneously the clicking operation of the mouse is simulated by judging the pressing action of the finger. The device comprises an image collection module, an image processing module and a wireless transmission module. First, the image of a user is collected at real time by a camera and then analyzed and processed by using an image processing algorithm to transform positions the user points to the screen and gesture changes into logic coordinates and control orders of the computer on the screen; and then the processing results are transmitted to the computer through the wireless transmission module. The invention provides a natural, intuitive and simple human-computer interaction method, which can realize remote operation of computers.
Owner:SOUTH CHINA UNIV OF TECH

Digital twin intelligent monitoring system for unmanned fully mechanized coal mining face of mine

The invention discloses a digital twin intelligent monitoring system and method for an unmanned fully mechanized coal mining face of a mine. The system for realizing the method is composed of a physical entity, a digital twin body, an intelligent gateway, a cloud server, a cloud database, a 5G base station, a machine vision device, a communication interface, a network switch and a video terminal.According to the method, a digital twin model is constructed, and perception analysis, analogue simulation, iterative optimization and decision control are carried out by using a convolutional networkdeep learning algorithm based on a three-dimensional visual virtual scene; real-time monitoring, intelligent sensing, accurate positioning and health prediction of the remote physical space mine unmanned fully mechanized coal mining face are achieved through the virtual space digital twin unmanned fully mechanized coal mining face based on data twinning and data driving. The problems of non-uniform communication interfaces, weak equipment integration capability, poor video monitoring image processing real-time performance and the like of the existing monitoring system are solved, and the real-time performance, the accuracy and the robustness of the intelligent monitoring system for the unmanned fully mechanized coal mining face of the mine are improved.
Owner:CHINA UNIV OF MINING & TECH (BEIJING)

Vehicle driving state evaluating method based on road-switching behavior detection

The invention discloses a vehicle driving state evaluating method based on road-switching behavior detection, comprising the steps of: carrying out video acquisition and real-time processing on front road conditions by using a camera so as to achieve purposes of monitoring dangerous driving behaviors of road-switching, long-time line-pressing driving and the like of a vehicle and immediately warn, i.e. arranging a camera facing the front direction inside the vehicle to obtain front road condition information; detecting and tracking a lane line; judging the current position state of the vehicle, if the vehicle is in a line-pressing state, carrying out state tracking to judge whether the vehicle is subjected to lane-switching operation or not; if the vehicle is subjected to the lane-switching operation, judging the behavior danger level of the vehicle according to the lane-switching times within a recent period of time; and if the total time in the line-pressing state within the recent period of time is m percent, making an alarm of the long-time line-pressing driving. For the vehicle active safe driving, by adopting a computer monocular vision and image processing technology, the invention has characteristics of simple system configuration, low cost, good real-time and capability of being applied in various climatic environments in the daytime and at night.
Owner:NANJING UNIV OF SCI & TECH
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