60 results about "Automated sequencing" patented technology

A method and device is disclosed for high speed, automated sequencing of nucleic acid molecules. A nucleic acid molecule to be sequenced is exposed to a polymerase in the presence of nucleotides which are to be incorporated into a complementary nucleic acid strand. The polymerase carries a donor fluorophore, and each type of nucleotide (e.g. A, T/U, C and G) carries a distinguishable acceptor fluorophore characteristic of the particular type of nucleotide. As the polymerase incorporates individual nucleic acid molecules into a complementary strand, a laser continuously irradiates the donor fluorophore, at a wavelength that causes it to emit an emission signal (but the laser wavelength does not stimulate the acceptor fluorophore). In particular embodiments, no laser is needed if the donor fluorophore is a luminescent molecule or is stimulated by one. The emission signal from the polymerase is capable of stimulating any of the donor fluorophores (but not acceptor fluorophores), so that as a nucleotide is added by the polymerase, the acceptor fluorophore emits a signal associated with the type of nucleotide added to the complementary strand. The series of emission signals from the acceptor fluorophores is detected, and correlated with a sequence of nucleotides that correspond to the sequence of emission signals.

The present disclosure provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles typically comprise steps of extension, ligation, and cleavage. In certain embodiments, the methods make use of extension probes containing phosphorothiolate linkages and agents capable of cleaving such linkages. Methods of determining information about a sequence using at least two distinguishably labeled probe families are provided, as are methods of performing multiple sequencing reactions on a single template. Automated sequencing systems, flow cells, image processing methods, and computer-readable media that store computer-executable instructions and/or sequence information that can be used in accordance with such methods are also provided. In certain embodiments, blocking oligonucleotides are provided to facilitate sequencing using disclosed methods.

New biochemical protocols for high throughput processing of mRNA samples into cDNA libraries with adaptor sequences compatible with automated sequencing systems are provided. The provided methods produces cDNA libraries which do not have 3′ bias associated with current cDNA library production methods. New methods for the production of DNA libraries from DNA are also provided.

Speech or musical performance is electronically analyzed using DSP techniques and is compared to a stored text script or musical score, to enable display of and automated sequencing through multiple pages of the stored script or score. The performer can modify timing of sequencing if desired, for example dependent on performance speed or musical complexity. Automated highlighting or marking of measures or text assists the performer in keeping track of progress through the performance. During or after the performance, electronic comparison of the performance to the score or script provides feedback to the performer, highlighting differences between the actual performance and the desired performance as described by the score or script. Additional capabilities enhancing the learning process include digital recording and playback of the actual performance, and/or accompaniment by synthesis or playback of digital recording of other performance parts (for example, other instruments in an ensemble or other actors in a play).

A system and method for automatically managing a multi-step process in which human providers are selected, typically for employment and dating services. The selection process includes background checking, testing, interviewing, and acceptance by the provider and selecting entity, through automated sequencing, coordinating, tracking, status reporting, and an optional human intervention. The invention includes a system and method for automatically scoring and ranking human providers by a set of selection criteria, typically multiple choice options. The system determines the relevance of the questions and answers and stores it in a continuously updating Knowledge-base. The answers may be spoken, written, or entered directly into the system. The system includes testing and interviewing human providers with respect to the selection criteria; and the resulting scoring information is stored and may be used in future assessments. The system requires a charging process for updating information based on usage or subscription to the system.

This disclosure provides a method and system for automated sequencing of vehicles in side-by-side drive-thru configurations via appearance-based classification. According to an exemplary embodiment, an automated sequencing method includes computer-implemented method of automated sequencing of vehicles in a side-by-side drive-thru, the method comprising: a) an image capturing device capturing video of a merge-point area associated with multiple lanes of traffic merging; b) detecting in the video a vehicle as it traverses the merge-point area; c) classifying the detected vehicle associated with traversing the merge-point area as coming from one of the merging lanes; and d) aggregating vehicle classifications performed in step c) to generate a merge sequence of detected vehicles.

The present invention provides a method for determining the sequence of a nucleic acid by successive cycles of duplex extension along a single-stranded template. This cycle includes the steps of: extension, ligation, preferably cleavage. In certain embodiments, the method utilizes an extension probe containing a phosphorothioate linkage and utilizes a substance suitable for cleaving such linkage. In certain embodiments, the method utilizes extension probes containing abasic residues or damaged bases, and utilizes substances suitable for cleaving linkages between nucleosides and abasic residues and/or suitable for Removes substances that damage bases in nucleic acids. The present invention provides methods for determining sequence information using at least two differentially labeled probe families. In certain embodiments, the method obtains less than 2 bits of information per cycle from each of the plurality of nucleotides of the template. In certain embodiments, the sequencing reaction is performed on templates attached to beads immobilized in or on a semi-solid support. The invention also provides labeled extension probe sets containing phosphorothioate linking or priming residues suitable for use in this method. In addition, the present invention includes removing the starting oligonucleotide and the extending strand and performing subsequent reactions with different starting oligonucleotides, thereby performing multiple sequencing reactions on one template. The invention also provides an effective method for preparing templates, especially for parallel sequencing of multiple different templates. The invention also provides methods for performing ligation and cleavage. The invention also provides novel libraries of nucleic acid fragments containing paired tags, methods for preparing microparticles to which multiple different templates (eg, containing paired tags) are attached, and for individually sequencing these templates. The invention also provides automated sequencing systems, flow chambers, image processing methods, and computer-readable media storing computer-executable instructions (such as performing image processing methods) and/or sequence information. In certain embodiments, sequence information is stored in a database.

The present invention relates to systems, compositions, and methods for nucleic acid sequencing and analysis in free-solution using protein polymer drag-tags. As such, the present invention provides protein-based molecular compositions that find use as drag-tags for use in sequencing and nucleic acid analysis methods and provides systems and methods for automated sequencing and analysis of nucleic acids in free solution.

The invention relates to a method for identifying designations. The method for identifying designations comprises following steps: (A1) detecting samples and outputting measurement values of elements of the samples; (A2) identifying designations according to the measurement values of elements in the samples and a designation element range; (A3) outputting designation identification results. The method has advantages such as automatic query and automatic ranking of designations.

The invention discloses an automatic production line for steel backing surface treatment. The automatic production line is used for transforming an existing steel backing surface treatment production line and comprises a steel backing cleaning machine, a steel backing sand blasting machine and a steel backing glue spraying machine and further comprises an automatic feeding and conveying device, a cleaning, sequencing and conveying device, a cleaning, discharging and conveying device, a sand blasting, feeding and conveying device, a sand blasting, discharging and conveying device, a transferring, sequenching and conveying device, a sorting, overturning and conveying device, a glue spraying and sequencing device and a drying, discharging and sorting device according to the production sequence. The automatic production line for steel backing surface treatment has the beneficial effects that full automation, automatic feeding, automatic sequenching, automatic transferring, automatic glue spraying, automatic drying and automatic sorting of steel backing surface treatment are achieved.

The invention provides an application program icon sequencing method and a mobile terminal, wherein the method comprises the following steps of collecting a signal in a preset type in the mobile terminal; judging the size of the collected signal value; generating a sequencing instruction according to the judging result; responding to the sequencing instruction; performing automatic sequencing on the application program icons in the desktop according to the set rule. By using the application program icon sequencing scheme provided by the embodiment of the invention, the mobile terminal can automatically sequence the application program icons according to the preset rule according to the generated instruction; the operation by a user is convenient; the time consumption is short.

The present disclosure provides a computer-implemented method and system for sequencing of a plurality of shipments in order of delivery. The method includes a first step of receiving a first set of data, a second set of data and a third set of data at sequence determination system. The method includes another step of determining one or more routes with facilitation of the first set of data at the sequence determination system. The method includes yet another step of analyzing the second set of data and the third set of data at the sequence determination system. The method includes yet another step of optimizing a final route form the one or more routes at the sequence determination system. The method includes yet another step of enabling a delivery associate of the one or more delivery associate to edit and approve the final route.

The present invention relates to a processing technology of stir-fried pumpkin seeds convenient to shell. The processing technology comprises the following steps: step one, feeding; step two, automatic sequencing; step three, controlling material blocking; step four, active separation, wherein in synchronization with the step three, a material blocking assembly drives a transmission assembly to synchronously transmit, the transmission transmission assembly drives a supporting rod to move, and inserting assemblies move towards gaps between two adjacent pumpkin seeds and realize separation of the integrally bonded pumpkin seeds in the moving process; step five, cutting, wherein a translation process of the inserting assemblies drives an opening mechanism to synchronously move to complete anautomatic cutting operation of middle parts of the pumpkin seeds; step six, discharging controlling; step seven, screening; and step eight, baking and frying. The processing technology solves technical problems that partial pumpkin seeds are adhered together under adhesion of the pulp and not separated thoroughly due to incomplete cleaning, and the targeted single cutting operation of the pumpkinseeds cannot be realized during the cutting operation.

The invention relates to a multi-stage self-sequencing liquid-controlled fracturing ball throwing device. The device includes a cover plate, a base, a seal cover, a rotary disk and a motor. The cover plate and the base are internally provided with conductive circuits, small fracturing balls are put in from the top, the weight of the balls are tested through a gravity sensor, a second hydraulic push mechanism pushes the balls to a ball delivery channel, the balls are sequentially located at the position of a baffle arranged in the radial direction of the rotary disk and located on the upper portion of a ball-throwing guide channel, according to the weight of the small fracturing balls, sequencing is conducted through the recursive algorithm, according to the sequence, the rotation angle of the motor is controlled so as to make the ball-throwing guide channel coincided with the axis of a ball throwing channel, and the small fracturing balls are pushed to the ball throwing channel through a first hydraulic push mechanism and enter a fracturing channel under the combined action of the high-pressure fluid negative pressure effect and the self gravity. According to the device, the several small fracturing balls can be automatically and sequentially thrown after being loaded at a time, the single-well multi-section continuous fracturing construction is effectively ensured, the safety risk of fracturing construction is reduced, and great engineering and economic benefits are generated.

The invention discloses a method and a system for automatically adjusting a file window sequence based on a file dependency relationship. Based on a VS code editor, the method comprises the followingsteps of generating an extension plug-in, adding a file sorting function instruction based on the extension plug-in, establishing and caching a dependency relationship between the files, and triggering the file sorting function instruction to automatically sort the file windows according to the dependency relationship. Through the method and the system, the function of sequencing and displaying the files from left to right according to the dependency relationship can be realized, and the coding experience is improved.

The invention discloses a counting device and method with automatic complementing and checking functions. The device comprises a PLC, a control display screen, an automatic sequencing device, a conveying device, an indexing rotating disc, a counting sensor, a servo motor, a weighing sensor and a weighing platform, wherein the indexing rotating disc is provided with a feeding port and a falling port; the automatic sequencing device is connected with the input end of the conveying device, and the output end of the conveying device is connected with the feeding port of the indexing rotating disc;the weighing sensor and the weighing platform are arranged below the falling port of the indexing rotating disc, the counting sensor is arranged at the falling port of the indexing rotating disc, andthe servo motor is connected with the indexing rotating disc; and the control display screen, the automatic sequencing device, the conveying device, the counting sensor, the servo motor and the weighing sensor are electrically connected with the PLC. The device has the advantages of being simple in structure, high in automation degree and high in counting accuracy.

The invention discloses an automatic identification machine for image recognition spring, and a working method thereof. The automatic identification machine is composed of a spring density ring automatic sequencing unit, a spring translation unit, a spring end face automatic locating unit, a spring automatic identification unit, a spring drying unit and a spring conveying unit. Springs are input through the spring density ring automatic sequencing unit, the front and back positions of spring density rings are judged through a pitch measuring camera, and the springs which do not meet the requirements are turned over through a spring density ring turnover mechanism; the sequenced springs are conveyed to the spring end face automatic locating unit through the spring translation unit; the position of an end face notch is measured through an end face notch measuring camera, and the springs are conveyed to the spring automatic identification unit through a spring pushing mechanism for identification; and the spring drying unit is used for drying, and the marked springs are conveyed through the spring conveying unit. According to the automatic identification machine, the problems that manual identification is slow, low in efficiency, prone to making mistakes, and not standard are solved, the cost is reduced, and the efficiency and quality are improved.

The invention discloses an automatic classification and arrangement method and equipment applied to pathological tissue slices, and belongs to the technical field of automatic classification of the pathological tissue slices. According to the method and the device, the plurality of samples in the automatic classification and arrangement equipment are automatically sorted according to the sorting information through the obtained sorting information, so that not only is the position adjustment efficiency of the plurality of samples improved, but also the problem of inaccurate sorting sequence caused by manual sorting is avoided.