905 results about "Fluorophore" patented technology

To provide a method for detecting the presence of multiple specific nucleic acid sequences or single nucleotide polymorphisms in a temperature-dependent fashion.SOLUTION: Provided is a method, in which an invasive oligonucleotide and a primary probe oligo (a 5' flap oligonucleotide) hybridize to a target strand nucleic acid to form an invasive cleavage structure cleavable by a FEN-1 endonuclease to release a cleaved 5' flap (primary cleaved flap); in a secondary reaction, the primary cleaved flap from the primary reaction hybridizes to a FRET cassette 1, a hairpin oligonucleotide labeled with a fluorescent dye and a quencher molecule, to form a second invasive cleavage structure that is cleavable by a FEN-1 endonuclease at a site between the fluorophore and a quencher; and cleavage of FRET cassette 1 separates the quencher from the fluorophore, such that fluorescence signal from the fluorophore can be detected.SELECTED DRAWING: Figure 1

Aspects of the present disclosure include methods for spectrally resolving light from fluorophores in a sample. Methods according to certain embodiments include detecting light with a light detection system from a sample having a plurality of fluorophores having overlapping fluorescence spectra and spectrally resolving light from each fluorophore in the sample with a generalized least squares algorithm. In some embodiments, methods include estimating the abundance of one or more of the fluorophores in the sample, such as on a particle. In certain instances, methods include identifying the particle in the sample based on the abundance of each fluorophore and sorting the particle. Methods according to some embodiments includes spectrally resolving the light from each fluorophore by calculating a spectral unmixing matrix for the fluorescence spectra of each fluorophore. Systems and integrated circuit devices (e.g., a field programmable gate array) for practicing the subject methods are also provided.

The invention belongs to the technical field of aquatic pathogen diagnosis, and particularly relates to a Taqman multiple fluorescent quantitative PCR (Polymerase Chain Reaction) detection system and kit for common vibrio pathogens in aquaculture as well as application and a detection method. The system comprises primers and probes for detecting four common vibrio pathogens in aquaculture, the 5'end of each probe is modified with different fluorophores, the 3 'end of each probe is modified with corresponding quenching groups, and the common vibrio pathogens comprise vibrio anguillarum Va, vibrio alginolyticus Val, vibrio harveyi Vh and vibrio scophthalmi Vsc.

The invention is based, at least in part, on the discovery that replacement of a key bond in the near-infrared fluorophore with a carbon-carbon bond conjugation to a targeting ligand results in vastly increased stability as compared to NIR fluorophores without such a bond, while preserving ligand and zwitterionic properties. In at least one aspect, the invention provides an imaging agent dye comprising a charge-balanced imaging agent conjugated to a targeting vector, wherein the targeting vector is a PSMA binding vector, such as dPSMA-617 or KUE, a FAP binding vector, a bombesin receptor binding vector, or a somatostatin receptor binding vector, and the charge-balanced imaging agent is ZW-800-1, ZW-830-1, or ZW-700-1-Forte.

The invention relates to the technical field of biosensing, in particular to a method for synergistically activating a CRISPR / Cas12a system based on a T-type structure and application of the method, the CRISPR / Cas12a system can be activated by utilizing two split DNA activation chains and crRNA to form the T-type structure, the activated CRISPR / Cas12a system has trans-cleavage activity, an FQ-ssDNA probe modified with an FAM fluorophore and a BHQ1 quenching group can be subjected to trans-cleavage, a fluorescence signal is recovered, and the fluorescence signal is recovered. Through reasonable design of sources of activation chains corresponding to different targets, detection targets can be expanded from traditional nucleic acid detection to non-nucleic acid targets such as proteins and metal ions, so that biosensing application of multiple targets is realized, in addition, design based on a T-type CRISPR / Cas12a synergistic activation strategy has great flexibility and universality, and the application prospect is wide. The method can be used together with various amplification strategies and detection platforms, and the sensitivity of the analysis method is effectively improved.

The present discloses systems and methods for tissue analysis, biopsy, and treatment utilizing at least exogenous fluorophores and near infrared light. A method is disclosed for optically determining the presence of abnormal cells, such as cancer, in tissue. The method includes applying one or more exogenous fluorophores into tissue in an animal body, wherein the exogenous fluorophores include a targeting moiety formulated to bond with a selected site on abnormal cells. The method includes illuminating the one or more exogenous fluorophores in the tissue with near infrared light from an elongated optical probe to generate fluorescent light from exogenous fluorophores present in the tissue. The method includes receiving fluorescent light generated from the one or more exogenous fluorophores in the tissue. The method includes determining if abnormal cells are present in the tissue based on the fluorescent light generated from the one or more exogenous fluorophores in the tissue.

The present disclosure relates in some aspects to methods, systems, and kits for sequencing a template nucleic acid molecule using nucleotide-fluorophore complexes. In some embodiments, each nucleotide-fluorophore complex comprises: (i) a single nucleotide attached to a core; and (ii) a plurality of fluorophores attached to the core; wherein each nucleotide-fluorophore complex comprises no more than one nucleotide. In some embodiments, the methods provided herein achieve improved signal intensity and sensitivity.

Disclosed are fluorescent tags comprising a cannabinoid fluorophore and compositions comprising the fluorescent tags.

The invention relates to the technical field of medical biology, in particular to a method for identifying and diagnosing PEDV (Porcine Epidemic Diarrhea Virus) and PoRV-A. Colloidal gold is used as a carrier, and a monoclonal antibody (mAb) for resisting PEDV and PoRV-A and an initiator are respectively modified on colloidal gold (Au) to prepare a probe (Au-I-mAb). An ELISA hole is coated with a monoclonal antibody (mAb), when a target virus exists, a probe is added to form a double-antibody sandwich compound (mAb-antibody-mAb-Au-I), a hairpin is added, and an initiation chain on the compound recognizes the matched hairpin and induces the hairpin to cross to generate HCR. Due to the fact that the hairpins are marked by different fluorophores, when H1 is in a closed hairpin structure, fluorescence on H1 is quenched by a quenching group, after H1 is subjected to ring opening through HCR, the fluorophores are away from the quenching group, and fluorescence is recovered. Fluorescence excitation is carried out by using corresponding emission wavelengths, and two target pathogens are finally detected at the same time by detecting output signals of different fluorophores.

The invention provides a probe capable of being used for fluorescence detection and RAA-LFD detection and application of the probe, the probe is used for isothermal amplification, a fluorophore, two quenching groups and tetrahydrofuran are arranged on the probe, and the fluorophore is located at the fifth end of a probe sequence; the second quenching group is positioned at the end 3 of the probe sequence; the first quenching group is located between the fluorophore and the second quenching group and is 12-20 basic groups away from the fluorophore; tetrahydrofuran is located between the fluorophore and the first quenching group and is 10-18 basic groups away from the fluorophore; the total length of the probe sequence is 27-32 basic groups, and compared with an existing RAA or RPA probe, the total length of the probe sequence is obviously shortened, design and use can be more convenient, the probe sequence is particularly suitable for RAA or RPA multiple amplification, multiple amplification of more than three times can be achieved, the probe can be used as a probe and a primer, the probe can be used for preparing a universal probe and a probe of an RAA-LFD system, and the probe sequence can be used for preparing the RAA-LFD system. The detection accuracy can be obviously improved, and the application prospect is wide.

The invention discloses a near-infrared two-region activatable probe as well as a preparation method and application thereof. The near-infrared second-region activatable probe comprises a near-infrared second-region fluorescent light-emitting unit and an analyte specific response unit; the near-infrared second-region light-emitting unit comprises a hemicyanine fluorophore structure; when the analyte specific response unit is chemically coupled with the near-infrared second-region light-emitting unit, the intramolecular charge transfer process of the light-emitting unit can be inhibited, and fluorescence quenching is caused; the analyte specific response unit is separated from the light-emitting unit after being subjected to specific reaction with an analyte, so that fluorescence is activated. The near-infrared two-region hemicyanine fluorophore selected by the near-infrared two-region activatable probe has higher fluorescence quantum efficiency, and the near-infrared two-region probe which can be activated by a specific analyte and has a high on-off ratio can be constructed by introducing an analyte specific response unit, so that high-temporal-spatial-resolution fluorescence detection of deep tissues can be realized; the method has a wide application prospect in the field of biomedical detection.

Systems and methods for calibrating an imager for unmixing of images with multiple fluorophores is described. One example method performed by a computing device includes receiving one or more single-color control images, a foreground channel, and a background channel. The single-color control images are images of the sample with a single fluorophore applied to the sample, and the foreground channel and the background channel are associated with the fluorophore. The method includes determining a difference between the foreground channel and the background channel, acquiring a foreground pixel mask from the difference, averaging a set of foreground pixels in the foreground pixel mask to generate a first spectrum, and subtracting an unstained spectrum from the first spectrum to generate a second spectrum. The second spectrum defines a spectral profile of the sample. A calibration slide and a method of preparing a calibration slide are also described.

The invention relates to the technical field of detection and analysis, and discloses a nanoparticle for detecting the concentration of methotrexate as well as a preparation method and application of the nanoparticle. The nanoparticles disclosed by the invention comprise hairpin DNA which is connected with the gold nanoparticles and is provided with a marking group and aptamer chain DNA which can be specifically recognized by methotrexate, and the aptamer chain DNA and walking chain DNA are hybridized into double-chain DNA which is connected to a magnetic bead, on one hand, the concentration of MTX is measured by marking a DNA chain of a fluorophore and collecting a fluorescence signal; on the other hand, after sodium chloride is added into the gold nanoparticles without DNA protection, the color of the gold nanoparticles changes under a high-salt condition, and the concentration of MTX is measured by collecting ultraviolet values. According to the nanoparticles provided by the invention, all parts interact with one another, signals are amplified, the effect of double detection of MTX concentration is exerted, and a powerful strategy is provided for accurately mastering the drug concentration in real time and improving the treatment safety and effect.

The invention relates to a coumarin-based fluorescent probe as well as a preparation method and application thereof in the aspect of HClO detection, and belongs to the technical field of HClO detection. The invention discloses a coumarin-based fluorescent probe W (O-(3-((2-morpholinyl ethyl) carbamoyl)-2-oxo-2hydrogen-benzopyran-7-yl) dimethyl thiocarbamate) prepared on the basis of an intramolecular charge transfer (ICT) mechanism, and a preparation method of the coumarin-based fluorescent probe W. The novel lysosome targeting fluorescent probe contains coumarin as a fluorophore, a morpholine group as a targeting group and a dimethyl thiocarbamate group as a recognition group, is a novel lysosome targeting fluorescent probe, and has excellent performances of high selectivity, low detection limit, low cytotoxicity, high resolution and the like in the aspect of HClO detection; the method can be used for detecting the content of endogenous and exogenous hypochlorous acid in living cells.

The invention discloses a double-recognition fluorescent molecular probe, a preparation method thereof and application of the double-recognition fluorescent molecular probe in detection of lead ions and / or glyphosate, and relates to the technical field of detection of lead ions and pesticide glyphosate. The double-recognition fluorescent molecular probe disclosed by the invention is a Schiff base type probe which takes dicyanoisophorone and benzoyl hydrazine as fluorophores. The invention also relates to application of the double-recognition fluorescent molecular probe in lead ion sensing detection. The complex of the double-recognition fluorescent molecular probe and lead ions can be used for sensing detection of glyphosate. The double-recognition fluorescent molecular probe provided by the invention has the advantages of easily available raw materials and simple operation. According to the present invention, the quantitative detection of the lead ions / or the glyphosate by the probe through the different functions displayed by the ultraviolet and fluorescence spectra is achieved, the reversibility is strong, the multi-time cyclic utilization can be achieved, the selectivity is good, the influence by other metal ions is not easily generated, the detection limit is low, and the excellent application imaging is provided in animal bodies such as cells, zebra fish and the like.

The invention provides an application of a double-stranded probe in detection of TTV, and particularly relates to an application of the double-stranded probe and a primer in detection of TTV. One chain of the double-chain probe comprises SEQ ID NO: 1 with a 5'end modified fluorophore, the other chain of the double-chain probe comprises SEQ ID NO: 2 with a 3 'end modified quenching group, and the primer sequences comprise SEQ ID NO: 3 and SEQ ID NO.4. When the double-chain probe and the primer are used for detecting TTV, 12 subtypes of TTV can be detected at the same time, the omission ratio is low, and the sensitivity is high.

A method for automatically ascertaining illumination brightnesses to be adjusted of at least two light sources for exciting at least one respective fluorophore in a sample to be imaged in a fluorescence microscope includes separately controlling, in terms of illumination brightness, each of the at least two light sources, detecting an image intensity of a microscopically imaged sample with at least two detectors, and automatically ascertaining the illumination brightnesses to be adjusted of the at least two light sources in such a way that a predefined setpoint of a signal-to-noise ratio is reached for each fluorophore. In order to ascertain the illumination brightnesses of the at least two light sources, cross-talk of a detector for different emission spectra of the fluorophores and / or cross-excitation of a fluorophore for different illumination spectra of the light sources are / is taken into account.

The invention relates to a single-molecule immunodetection method and a single-molecule chip, and the single-molecule immunodetection method comprises the following steps: slowly adding a microsphere reagent mixed with different codes into the chip, capturing and fixing microspheres by a microsphere clamping groove at the side edge of a main flow channel in the flowing process of the microspheres in the chip, the residual reagent passes through the main flow channel and the secondary flow channel and then is collected to a waste liquid collecting area; the method comprises the following steps: introducing an antigen to be detected and a mixed fluorescent substance with different detection items into a chip, and then introducing a cleaning solution to wash the redundant antigen and the free mixed fluorescent substance; counting the number of the luminous microspheres and the fluorescence brightness through a Poisson distribution probability formula, and establishing a standard curve to obtain the concentration of the to-be-detected sample. The microspheres are captured and fixed by the microsphere clamping grooves to the maximum extent, the loss amount of the microspheres is low, an antigen to be detected is introduced into the chip, the immunoreaction is fully carried out, the detection sensitivity is high, the detection operation process is highly simplified, the detection cost is reduced, the detection efficiency is improved, and multiple target molecule detection can be realized at the same time.

Multiple fluorophores within a sample can be imaged by merging a plurality of beams from different wavelength light sources of excitation light into a single path, directing the excitation light into the sample, and detecting light emitted by the fluorophores within the sample on two different arrays of pixels (e.g., two regions within a single camera sensor chip). The light sources are activated during respective timeslots, and captured image data is processed. For at least one of the timeslots, the processing of the image data comprises using the image data captured using the first array of pixels to detect a presence of a given fluorophore, and using the image data captured using the second array of pixels to detect a presence of a different fluorophore. This arrangement enables a system that includes only N light sources to image more than N fluorophores.

The invention relates to the technical field of organic synthesis and the technical field of fluorescent materials, in particular to a boron coordination salicylhydrazone double-state emission fluorophore as well as a preparation method and application of the boron coordination salicylhydrazone double-state emission fluorophore. The structure of the boron coordination salicylazone double-state emission fluorophore is shown as I, II or III; wherein R1 is selected from one of H, halogen atoms, diethylamino and thienyl, R2 is selected from one of H, halogen atoms, diethylamino and thienyl, and R3 is selected from one of aryl, 9, 9 '-spirobifluorenyl, alkoxy and halogen atoms. The boron coordination salicylazone double-state emission fluorophore is diversified in structure and good in solubility, has excellent photophysical properties and extremely high quantum yield, and also shows excellent biocompatibility and remarkable liposome targeting property at the same time. Moreover, the preparation method of the boron-coordinated salicylazone double-state emission fluorophore adopts a one-pot two-step method, is simple and convenient, is high in yield, is green and environment-friendly, and has a wide application prospect.

The present invention relates to a method for determining the efficiency of RNA encapsulation in lipid nanoparticles (LNPs). In some embodiments, a method according to the invention comprises the steps of a) contacting a sample comprising RNA encapsulated in LNP with a first fluorophore and a second fluorophore, thereby forming a fluorophore-RNA complex, and b) detecting a fluorescence signal of the complexed first and second fluorophore, wherein the first fluorophore permeates the LNPs and wherein the second fluorophore does not permeate the LNPs.

The invention discloses a polyimide film with an aggregation-induced emission effect and a preparation method thereof, and belongs to the field of flexible electronic luminescence. Through the technical means of side chain grafting, the bonding of TPE fluorophore and PI polymer on the molecular level is realized for the first time, the PI film material is endowed with good photoluminescence performance, and meanwhile, the PI film material has outstanding mechanical strength and thermal stability. In addition, the invention can be expanded to other polymer systems, and is expected to solve the scientific problem that the luminescent property, the thermal property and the mechanical property of the current flexible display luminescent material cannot be considered at the same time. Therefore, according to the technical scheme provided by the invention, diversified design ideas and raw material bases can be provided for molecular structure design and efficient preparation of the PI containing the TPE element and the film material of the PI, and the application of the PI material in the field of flexible electronics can be promoted and deepened.

Provided is a method for simply and quickly screening for proteins associated with neurodegenerative diseases, or compounds that are useful for the treatment or prevention of neurodegenerative diseases. The method uses a probe formed by covalently bonding an environmentally responsive fluorophore to a dipeptide repeat sequence comprising a repeating unit selected from the group consisting of proline-arginine, glycine-arginine, proline-alanine, glycine-alanine and glycine-proline.

The invention belongs to the technical field of analysis and detection, and particularly relates to a beta-galactosidase response type hemicyanine near-infrared fluorescent probe, a preparation method thereof and application of the beta-galactosidase response type hemicyanine near-infrared fluorescent probe as a tumor organ imaging probe. The near-infrared fluorescent probe provided by the invention takes a water-soluble hemicyanine skeleton as a fluorophore, so that the biocompatibility of the near-infrared fluorescent probe is improved; based on the principle that beta-galactose can be selectively cut by beta-galactosidase, a galactose structure with a self-leaving group is used as a response group, selective quick response to the beta-galactosidase is achieved, and high sensitivity to the beta-galactosidase is shown. The near-infrared fluorescent probe provided by the invention can emit near-infrared fluorescence with long wavelength, has lower background fluorescence and good penetration depth, and can be directly used for detecting organoids containing the matrigel without eliminating the matrigel, so that the organoids are prevented from being damaged or additional experimental variables are prevented from being introduced in the process of eliminating the matrigel.

In some instances, a system for rapid spectral unmixing using spectrally interpolated background reduction (SIBR) is provided. The system comprises a device comprising an excitation light source and a detector. The system further comprises a control system configured to: obtain a plurality of excitation spectra for a plurality of fluorophores that are used to stain a sample; determine a plurality of chosen wavelengths and a plurality of chosen light intensities for the plurality of fluorophores; obtain a first detection of the sample based on using a set of first wavelengths from the plurality of chosen wavelengths and a set of first light intensities; obtain a second detection of the sample based on using a set of second wavelengths from the plurality of chosen wavelengths and a set of second light intensities; and output a SIBR quantity.

The invention provides a multi-focus excitation confocal fluorescence microscopic imaging flow cytometry screening method. The method is characterized in that a laser beam generates three laser beams through a spatial light modulator, and the three laser beams are coherently superposed in a focal plane of a microscope objective to generate a two-dimensional optical lattice light field. And after the other laser beam is shaped by the cylindrical lens, linear laser optical tweezers are realized through the microscope objective, cells to be detected in an imaging detection area of the micro-flow chip are captured, and accurate active optical control on the rotation angle of the cells to be detected is realized. In a cell rotation process, a two-dimensional optical lattice light field rapidly scans in a focal plane at different angles, and a fluorophore in the cell is excited to generate a fluorescence signal. The confocal pinhole array installed at the position conjugate with the focal plane eliminates background fluorescence, a high-spatial-resolution fluorescence image of a three-dimensional structure in the cell is obtained, and cell screening is achieved. The method provided by the invention has the characteristics of high detection rate, high accuracy and the like, and has wide application prospects in the research fields of biology, medicine and life science.

In an analysis method and an analysis system for detecting fluorescences from each of a plurality of light-emitting points in a plurality of wavelength bands in order to identify fluorescence emissions of a plurality of types of fluorophores from the plurality of light-emitting points, spatial crosstalk and spectral crosstalk are present between the plurality of light-emitting points and between the plurality of wavelength bands, and then performance of the identification is reduced. The spatial crosstalk and the spectral crosstalk are eliminated and concentrations of each of the plurality of types of fluorophores at each of the plurality of light-emitting points are derived by inputting all detection signals in the plurality of wavelength bands for the plurality of light-emitting points to a predetermined arithmetic operation expression.

The invention discloses a compound, a kit and application thereof, and relates to the technical field of biomedicine. The compound comprises an electron acceptor aromatic unit, an optional electron donor aromatic unit and a shielding unit, and the shielding unit shields intermolecular interaction of the electron acceptor aromatic unit and / or the electron donor aromatic unit. Wherein the electron acceptor aromatic unit, the electron donor aromatic unit and the shielding unit are connected through covalent bonds. The compound can be used as a molecular near-infrared-II fluorophore, and has higher quantum yield and good biocompatibility. The compounds emit fluorescence in the range of 900-1400 nanometers, the intermolecular and intramolecular interaction of the conjugated skeleton is weakened, and the quantum yield is improved. The high quantum yield, biocompatibility and near-infrared II emission of the compound provide opportunities for in-vivo application of near-infrared II imaging by using molecular fluorophores.

The invention discloses a fluorescent probe as well as a preparation method and application thereof. The invention provides a series of fluorescent probes with novel structures, and the fluorescent probes take 2-(isoquinoline-1-yl) pyrrole boron difluoride as fluorophores, so that the fluorescence quantum yield of the probes is improved, the detection resolution of nitric oxide is increased, and the detection sensitivity is improved. The fluorescent probe disclosed by the invention has the characteristics of large Stokes displacement, high fluorescence quantum yield, good selectivity and short response time.

The present invention is applicable to the technical field of near-infrared zone II imaging, provides a preparation method of a cyanine dye variant and a cyanine protein composite fluorophore, successfully prepares a series of cyanine dye-protein composite probes by using a protein encapsulation strategy, and forms a covalently bound complex by protein encapsulation and its application, wherein the covalently bound complex is formed by covalently linking a cyanine dye molecule and a protein; compared with the existing near-infrared zone II fluorescent probe, the composite of the present invention has better biocompatibility and luminescent behavior, a longer wavelength, good optical stability, high brightness, and superior imaging effect in fluorescent imaging applications.