The invention discloses a human ALK
fusion gene detection primer set and a detection kit. The primer set comprises one or more detection primers in sixteen ALK
fusion gene types A01 to A16 and
Taqman probes corresponding to the
gene types. As many as sixteen
fusion gene types except for EML4 can be detected through the detection kit formed by the primer set and the probes, the requirement for the sample
RNA obtaining quality is low, and the ideal detection effect can be achieved through samples in different existence forms; by means of the kit, an operator only needs to directly feed extracted sample
RNA once, inverse transcription and PCR amplification are carried out in one
closed tube reaction system, and the
pollution probability and the result error probability are reduced. One-time detection only takes eighty minutes. The human ALK fusion
gene detection primer set and the detection kit have the remarkable advantages that the number of the detection types is large, sensitivity is high, experiment operation is simple, the period is short, the human ALK fusion
gene detection primer set and the detection kit are safe and nontoxic, and cost is low.