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510 results about "Closed tube" patented technology

Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon

The present invention provides labeled nucleic acid amplification oligonucleotides, which can be linear or hairpin primers or blocking oligonucleotides. The oligonucleotides of the invention are labeled with donor and / or acceptor moieties of molecular energy transfer pairs. The moieties can be fluorophores, such that fluorescent energy emitted by the donor is absorbed by the acceptor. The acceptor may be a fluorophore that fluoresces at a wavelength different from the donor moiety, or it may be a quencher. The oligonucleotides of the invention are configured so that a donor moiety and an acceptor moiety are incorporated into the amplification product. The invention also provides methods and kits for directly detecting amplification products employing the nucleic acid amplification primers. When labeled linear primers are used, treatment with exonuclease or by using specific temperature eliminates the need for separation of unincorporated primers. This "closed-tube" format greatly reduces the possibility of carryover contamination with amplification products, provides for high throughput of samples, and may be totally automated.
Owner:MILLIPORE CORP

Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon

The present invention provides labeled nucleic acid amplification oligonucleotides, which can be linear or hairpin primers or blocking oligonucleotides. The oligonucleotides of the invention are labeled with donor and / or acceptor moieties of molecular energy transfer pairs. The moieties can be fluorophores, such that fluorescent energy emitted by the donor is absorbed by the acceptor. The acceptor may be a fluorophore that fluoresces at a wavelength different from the donor moiety, or it may be a quencher. The oligonucleotides of the invention are configured so that a donor moiety and an acceptor moiety are incorporated into the amplification product. The invention also provides methods and kits for directly detecting amplification products employing the nucleic acid amplification primers. When labeled linear primers are used, treatment with exonuclease or by using specific temperature eliminates the need for separation of unincorporated primers. This "closed-tube" format greatly reduces the possibility of carryover contamination with amplification products, provides for high throughput of samples, and may be totally automated.
Owner:MILLIPORE CORP

Dust separation system

A vacuum cleaner having a nozzle, a handle pivotally attached to the nozzle, and a suction motor that has an inlet, and is adapted to generate a working air flow through the nozzle. The vacuum includes a separation system having an outer wall and a closed tube having at least a portion of its length located within the wall, and forming a separation chamber between the wall and the closed tube. The separation chamber has an inlet, in communication with the nozzle, that is adapted to impart a tangential component to the air flow as it flows through the separation chamber. A hollow tube is generally coaxially aligned with the closed tube and has a tube inlet at an end adjacent the closed tube and a tube outlet at an end opposite the closed tube. The tube outlet is in fluid communication with the suction motor inlet.
Owner:ELECTROLUX HOME CARE PRODS

Electronics assembly and heat pipe device

An electronics assembly is provided having a heat pipe device for cooling electronics. The assembly includes a substrate and an electronics package supported on the substrate. The assembly also includes a heat pipe device in thermal communication with an exposed surface of the electronics package. The heat pipe device includes a thermal conductive pipe having an internal volume and an open end. The heat pipe device also includes a thermal conductive end cap positioned to close the open end of the pipe. The end cap has an outer surface for receiving in thermal communication an electronics package. The heat pipe device further includes a cooling fluid disposed in the internal volume of the pipe for transferring thermal energy from the end cap to the outside environment.
Owner:DELPHI TECH IP LTD

Microneedle array device and its fabrication method

A microneedle array device and its fabrication method are provided. The microneedle array device comprises a supporting pad and plural of microneedles. Each microneedle has a top portion with a via thereon, thereby the microfluid may flow in or out. The intersection between the top portion and the inner tube of a microneedle forms a convex needle structure, and is almost perpendicular to the upper surface. For each microneedle, a hollow closed tube is formed between the top portion and the supporting pad. The fabrication method uses the substrates with high transmittance and plural of convex area thereon as the upper and lower caps, and applies a photolithography process to fabricate a microneedle array mold. It then sputters or electroplates metal material on the mold. The microneedle array is formed after having taken off the mold. It is a simple fabrication process.
Owner:IND TECH RES INST

Telescoping closed-tube sampling assembly

A clinical diagnostic sample analyzer for analyzing a sample of a patient is disclosed. The analyzer includes a telescoping closed-tube sampling assembly with a sample probe concentrically housed within a piercing probe and a venting mechanism. The closed-tube sampling assembly is used for aspirating a sample from a sample tube for analysis by a clinical diagnostic sample analyzer.
Owner:INSTR LAB

Dust separation system

An upright vacuum cleaner having a nozzle adapted to be traversed on a surface to be cleaned, and having an internal passage defined by a nozzle inlet positioned to be substantially adjacent the surface to be cleaned and a nozzle outlet remote from the nozzle inlet. A handle is pivotally attached to the nozzle, and a suction motor is provided in the nozzle or the handle. The suction motor has a suction motor inlet, and is adapted to generate a working air flow through the nozzle and into the suction motor inlet. The vacuum includes a separation system having an outer wall, a closed tube having at least a portion of its length located within the outer wall and forming a separation chamber between the outer wall and the closed tube, a separation chamber inlet in fluid communication with the nozzle outlet and adapted to impart a tangential component to the working air flow as it flows through the separation chamber, and a hollow tube that is generally coaxially aligned with the closed tube and has a tube inlet at an end adjacent the closed tube and a tube outlet at an end opposite the closed tube. The tube outlet is in fluid communication with the suction motor inlet. The device also includes a collection chamber for receiving dirt separated from the working air flow.
Owner:ELECTROLUX HOME CARE PRODS

Method and apparatus for removing heat

A device includes a liquid metal and a ferrofluid contained in a closed tube. Many electrode groups are connected to the closed tube. A feedback device is connected to the electrode groups. The feedback device switches power to each electrode group in series to circulate the liquid metal and move the ferrofluid in the closed tube.
Owner:INTEL CORP

Wound irrigation apparatus and method

A dispenser cap for combination with a standard flexible irrigation solution bottle to make a portable wound irrigation dispenser includes a collar threadably engageable with the irrigation bottle, and closed at one end by a wall defining a tubular neck. The tubular neck forms a flow path from the bottle to a splash shield including a nozzle for directing irrigation solution. A valve is disposed in the flow path for opening and closing this flow path. A combination apparatus includes the flexible irrigation solution bottle, which may be inverted and squeezed in order to direct a jet of cleansing liquid into a wound. A single use version of the apparatus includes a valve which is closed in a storage and shipping configuration of the apparatus, and an indicator which is freed within the transparent or translucent flexible bottle when the valve is opened, thus irreversibly indicating that the apparatus has been used. An alternative embodiment of the apparatus includes a safety valve which also prevents misuse of the apparatus without the splash shield installed.
Owner:LANDAU SERGIO +1

Hole bottom freezing cord coring drill and coring method thereof

The invention relates to a hole bottom freezing cord coring drill and a coring method thereof. In the invention, liquid nitrogen is taken as a refrigerant; the liquid nitrogen absorbs heat in the gasification process so as to freeze a core at a hole bottom and inhibit a natural gas hydrate from being decomposed. The drill comprises an outer tube assembly and an inner tube assembly; and in the process of acquiring the core, a sand hitch is utilized to drag the inner tube assembly out from the outer tube assembly, an end cover and a clamp spring are dismounted, a semi-closed tube and a core sample are pumped out together from a core tube, the two ends of the semi-closed tube are sealed by rubber plugs, and the core sample and the semi-closed tube are stored in a liquid nitrogen hold-up vessel or a high-pressure container together. The invention has the advantages that rapid freezing can be realized, and the freezing effect is good; since cord coring and hole bottom freezing are combined, rapid coring without lifting the drill is achieved; in the drilling process, the inner tube assembly does not rotate, thus damages on a hydras core caused by mechanical force generated by rotation of the drill is avoided to a greater degree, the percentage of coring, the core integrity degree and the representativeness are more effectively improved; and semi-closed tube type rapid ground surfacesampling is adopted, thus the core can be rapidly obtained from the ground surface.
Owner:JILIN UNIV

Electric machine with thermosiphon-type cooling system

An electric machine includes a housing for accommodating a rotatably supported rotor and a stationary stator, and a cooling device for cooling heat-generating components of the rotor and the stator. The cooling device includes a closed-flow system having an evaporator disposed in the housing, a condenser disposed outside the housing at a geodetically higher location, and a closed tube assembly, which connects the condenser and the evaporator, for circulating a coolant using a thermosiphon effect as a result of a difference in density between vapor and liquid portions of the coolant. The closed-flow system contains a liquid-vapor mixture of the coolant in a saturated state and includes a first tube for conducting coolant to the evaporator and a second tube for conducting coolant away from the evaporator. The first tube has hereby a cross section which is smaller than a cross section of the second tube.
Owner:SIEMENS AG

Tube car, network of tubes, personal transport system, and control system and control method thereof

The present invention provides a new personal transport system for passengers and goods, which comprises: a tube car comprising a self-powered closed tube car carrying passengers or goods from door to door; and a network of tubes comprising a plurality of tubes, the said network of tubes combined with the existing blocks or buildings, with each tube in the network of tubes provided with an upper rail and / or a lower rail for supporting and / or guiding the said tube car carrying passengers or goods. According to the invention it is possible to achieve a door-to-door transport between two buildings. In comparison to other traffic systems, the inventive system's velocity, line capacity, safety and reliability, ability of resisting disasters and comfortableness are much increased while its consumption of energy, construction cost, occupied space, operating cost, noise and visual pollution are greatly reduced. Furthermore, the transport system of the invention is easy to put into a standardized production, quick to be laid, removed and reused.
Owner:YANG NANZHENG

Tube car, network of tubes, personal transport system, and control system and control method thereof

The present invention provides a new personal transport system for passengers and goods, which comprises: a tube car comprising a self-powered closed tube car carrying passengers or goods from door to door; and a network of tubes comprising a plurality of tubes, the said network of tubes combined with the existing blocks or buildings, with each tube in the network of tubes provided with an upper rail and / or a lower rail for supporting and / or guiding the said tube car carrying passengers or goods. According to the invention it is possible to achieve a door-to-door transport between two buildings. In comparison to other traffic systems, the inventive system's velocity, line capacity, safety and reliability, ability of resisting disasters and comfortableness are much increased while its consumption of energy, construction cost, occupied space, operating cost, noise and visual pollution are greatly reduced. Furthermore, the transport system of the invention is easy to put into a standardized production, quick to be laid, removed and reused.
Owner:YANG NANZHENG

Multiple function spray nozzle

InactiveUS20030042331A1Expand the area of ​​influenceMovable spraying apparatusSpray nozzlesClosed tubeMultiple function
A spray nozzle has a head, a cap and a middle plate. The head has a discharge channel. The cap is rotatably mounted on the head. A circular baffle is formed on the cap and has a space defined inside the baffle. Multiple nozzle units and a closed tube communicating with the space in the baffle are formed on the baffle. Multiple bores are defined in the cap and communicate with the space in the baffle. Multiple misting nozzles are mounted on the cap outside the baffle. The middle plate is secured in the cap. Multiple through holes are defined in the plate in a circle. Each nozzle unit on the baffle and the closed tube aligns with one of the through holes. Accordingly, the water can be sprayed from the spray nozzle in different forms. The using of the spray nozzle becomes more versatile.
Owner:LU KUO CHOU

Two-piece side and floor panel arrangement for box assembly

An improved vehicle box assembly with an upright front or side panel defined by a monolithic one-piece roll-formed steel sheet member having a desired three-dimensional configuration. The sheet steel member is roll-formed in the lengthwise direction thereof. The panel has a channel, preferably a closed tubular channel on the front panel, roll-formed along one edge of the sheet steel during forming of the panel. The closed tubular edge rail is preferably closed by a seam weld which extends lengthwise therealong and is performed in line with the roll-forming operation. The roll-formed panel is preferably provided with a flat shelf deformed horizontally to extend lengthwise along the panel downwardly from the top rail.
Owner:PULLMAN IND

Nested Multiplex Amplification Method for Identification of Multiple Biological Entities

The present invention provides a novel molecular method for the simultaneous identification and semi-quantification of multiple targeted biological entities from amongst a plurality. This invention discloses a method based on a multiplex nested amplification reaction in a single closed tube. The first amplification reaction relies on a set of large oligonucleotides for the amplification of common loci in all the targeted biological entities. The second nested amplification reaction relies on a set of short oligonucleotide primers that amplifies specific nucleotide sequences from all the amplicons previously produced in the first amplification reaction and generates an amplified product pattern capable of identifying each targeted biological entity. This method offers fast and accurate simultaneous identification of many targeted biological entities in any sample.
Owner:TAAG GENETICS

Package with integral plug

A package is disclosed having integral means to seal an opening in a packaged product, such as a capless writing instrument, in order to protect material within the packaged product, such as ink, from excessive exposure to air while stored in the package. The package may be of any type used to contain articles of merchandise, including, without limitation, a blister package, a clamshell package, a closed tube, or a box. A plug portion attached to or molded into the interior of the package is sized and positioned so that it fits sealingly in an opening of a product contained within said package in order to prevent or minimize passage of air through said opening. Also disclosed is a package insert for holding an article of merchandise securely within a package while sealing an opening in the merchandise.
Owner:INNODESK

System, apparatus, and method for closed tube sampling and open tube sampling for automated clinical analyzers

A centrifuge to which sample tubes can be introduced while the centrifuge is in motion. The centrifuge comprises a carousel having an upper portion and a lower portion. The upper portion of the carousel has a plurality of positions for sample tubes for a centrifugation operation, a plurality of drive mechanisms attached to the upper portion of the carousel, a movable element mounted upon each drive mechanism, the movable element capable of traversing the length of the drive mechanism when the drive mechanism is actuated, a sample tube-holding assembly comprising a sample tube holder and a bearing attached to each movable element, and at least one balancing element capable of contributing to a force vector that cancels an imbalance vector generated by rotation of the centrifuge.
Owner:ABBOTT LAB INC

Method and apparatus for removing heat

A device includes a liquid metal and a ferrofluid contained in a closed tube. Many electrode groups are connected to the closed tube. A feedback device is connected to the electrode groups. The feedback device switches power to each electrode group in series to circulate the liquid metal and move the ferrofluid in the closed tube.
Owner:INTEL CORP

Electric machine with thermosiphon-type cooling system

An electric machine includes a housing for accommodating a rotatably supported rotor and a stationary stator, and a cooling device for cooling heat-generating components of the rotor and the stator. The cooling device comprises a closed-flow system having an evaporator disposed in the housing, a condenser disposed outside the housing at a geodetically higher location, and a closed tube assembly, which connects the condenser and the evaporator, for circulating a coolant using a thermosiphon effect as a result of a difference in density between vapor and liquid portions of the coolant. The closed-flow system contains a liquid-vapor mixture of the coolant in a saturated state and includes a first tube for conducting coolant to the evaporator and a second tube for conducting coolant away from the evaporator. The first tube has hereby a cross section which is smaller than a cross section of the second tube.
Owner:SIEMENS AG

Plane-structure InGaAs array infrared detector

The invention discloses a novel plane-structure InGaAs array infrared detector. The structural design of the infrared detector is as follows: a shallow isolation groove is formed around an array photosensitive surface on an NIN-type epitaxial wafer by etching; a PN junction area of the photosensitive surface is formed by closed tube diffusion, and a guard ring integrated with the shallow isolation groove is formed; and guard ring electrodes and annular covering electrodes are formed by thickening Cr / Au. The novel plane-structure InGaAs array infrared detector has the advantages that the shallow isolation groove and the guard ring with the integrated design can effectively inhibit cross talk between adjacent photosensitive surfaces of an array device and enlargement of the photosensitive surfaces, and the enlargement of the photosensitive surfaces can be further inhibited and the photosensitive surfaces can be accurately defined if the infrared detector is aided with small diffusion holes and the annular covering electrodes. In the plane extension wavelength InGaAs array device, the shallow isolation groove can also help effectively inhibit drain current between P electrodes of adjacent photosensitive surfaces caused by lattice mismatching of materials.
Owner:SHANGHAI INST OF TECHNICAL PHYSICS - CHINESE ACAD OF SCI

ARMS fluorescent quantitative PCR-based gene mutation kit and method thereof

The invention relates to an ARMS fluorescent quantitative PCR-based gene mutation kit and a method thereof. Compared with present gene polymorphism detection kits and methods thereof, the enhanced ARMS fluorescent quantitative PCR-based gene polymorphism mutation kit and the method thereof have the advantages of strong specificity, high sensitivity, low cost, highly reliable result, low background, easy determination of the result, ascendant operation and use, fast and convenient fluorescent quantitative PCR, closed tube detection in the whole course, pollution reduction, and reduction of the unnecessary cost and workload. The fluorescent quantitative PCR can realize the characterization of the amplification efficiency by using a CT value, so the method can realize the detection of the nuclear gene polymorphism heterozygote genotype conditions, and makes the result accurately and reliably determined.
Owner:JIANGSU MACRO&MICRO TEST MED TECH CO LTD

High throughput flash purification stand and cartridge

A chromatography cartridge for use in a chromatography stand comprises a tube for containing chromatography media. An end cap is adapted to fit in an end of the tube for closing the end of the tube. The end cap has a generally cylindric body having an external axial surface at least partially received in the tube and an outer face. At least one protrusion extends from the external axial surface of the body. The protrusion is shaped and arranged to contact an interior surface of the tube to form a fluid-tight connection between the end cap and the tube.
Owner:SIGMA ALDRICH CO LLC

Electronics assembly and heat pipe device

An electronics assembly is provided having a heat pipe device for cooling electronics. The assembly includes a substrate and an electronics package supported on the substrate. The assembly also includes a heat pipe device in thermal communication with an exposed surface of the electronics package. The heat pipe device includes a thermal conductive pipe having an internal volume and an open end. The heat pipe device also includes a thermal conductive end cap positioned to close the open end of the pipe. The end cap has an outer surface for receiving in thermal communication an electronics package. The heat pipe device further includes a cooling fluid disposed in the internal volume of the pipe for transferring thermal energy from the end cap to the outside environment.
Owner:DELPHI TECH IP LTD

Primer-probe composition, kit and detection method for detecting seven kinds of hot-spot mutation of KRAS gene of humans

The invention discloses a primer-probe composition for detecting seven kinds of hot-spot mutation of KRAS gene of humans. The primer-probe composition comprises 7 pairs of specific primers and one kind of specific probes, wherein the 7 pairs of specific primers are respectively prepared from 7 kinds of ARMS primers and one kind of downstream primer. The invention further discloses a kit containing the primer-probe composition, and a method for detecting mutation by using the kit. The primer-probe composition disclosed by the invention is high in sensitivity and specificity; when the kit is used, the detection processes are all reactions carried out in closed tubes, so that the pollution is significantly reduced, and besides, a UNG enzyme anti-pollution system and an internal control system are added, so that more accurate and stable typing detection can be performed on samples, and the results are guaranteed to be genuine and credible; the detection method is fast, the detection can be completed within 100 minutes, the result judgment is simple and objective, and the analysis is convenient.
Owner:SHANDONG VIGENE BIOSCI

Human ALK fusion gene detection primer set and detection kit

InactiveCN105039580AMultiple detection fusion typesSpecify the fusion typeMicrobiological testing/measurementDNA/RNA fragmentationGene typeReaction system
The invention discloses a human ALK fusion gene detection primer set and a detection kit. The primer set comprises one or more detection primers in sixteen ALK fusion gene types A01 to A16 and Taqman probes corresponding to the gene types. As many as sixteen fusion gene types except for EML4 can be detected through the detection kit formed by the primer set and the probes, the requirement for the sample RNA obtaining quality is low, and the ideal detection effect can be achieved through samples in different existence forms; by means of the kit, an operator only needs to directly feed extracted sample RNA once, inverse transcription and PCR amplification are carried out in one closed tube reaction system, and the pollution probability and the result error probability are reduced. One-time detection only takes eighty minutes. The human ALK fusion gene detection primer set and the detection kit have the remarkable advantages that the number of the detection types is large, sensitivity is high, experiment operation is simple, the period is short, the human ALK fusion gene detection primer set and the detection kit are safe and nontoxic, and cost is low.
Owner:武汉海吉力生物科技有限公司

A curtain type membrane assembly

The invention relates to a curtain-type film assembly used in flim bioreactor. In order to settle present problem in technology, the invention provides a curtain-type film assembly, and comprises hollow fiber microporous membrane thread and collecting tube, which is characterized in that the hollow fiber flim is in 'U' shape, with open end connected with upper collecting tube, the lower of U-shape is free end, between lower membrane threads slotting tube is located, which is thinner than collecting tube and taken as inset tube. Inset tube connected with aerated conduit and perforated-up. Inset tube and lower aerated conduit forming a closed tube circuit, the lower of membrane threadn is in the circuit. The curtain-type film assembly supported by the invention has the following merits: no-contamination, no activated sludge between membrane thread, convenience on cleaning membrane thread, and it can improve dewatering efficiency of film assembly.
Owner:TIANJIN MOTIAN MEMBRANE ENG & TECH

Method, reagent, primer and probe for quickly detecting Ebola viruses under constant-temperature and isothermal conditions

The invention discloses a method, a reagent, a primer and a probe for quickly detecting Ebola viruses under constant-temperature and isothermal conditions. The method, the reagent, the primer and the probe have the advantages that the Ebola viruses can be quickly, sensitively and accurately detected by the aid of the method in real time under the constant-temperature and isothermal conditions, nucleic acid targets can be quickly detected in instruments with fluorescence detection functions under the combined actions of the specific primer, the specific fluorescence probe, six engineering enzymes and other chemical constituents, closed-tube detection can be guaranteed by strict experiment operation steps, and aerosol pollution can be effectively prevented; the method, the reagent, the primer and the probe are applicable to detecting the Ebola viruses by the aid of diversified fluorescence detection devices.
Owner:ZHEJIANG SHANCE HEQISHI BIO SCI& TECH CO LTD

Method for recovering mixed rare earth chlorides from neodymium iron boron permanent magnet material scraps

The invention provides a method for recovering mixed rare earth chlorides from neodymium iron boron permanent magnet material scraps, which is characterized in that: grinding the neodymium iron boron permanent magnet material scraps in an environment of an inert gas to obtain a powdered material, adding a proper amount of carbon powder into the powdered material, introducing a dry chlorine gas into the mixed powder to perform chlorination and performing two closed-tube chemical vapor transport processes for the chlorinated product to obtain the mixed rare earth chlorides which contain over 95 percent of rare earth and anhydrous ferric chloride with a purity of about 98 percent respectively; and using oxalate to perform precipitation and obtaining cobalt oxide with a purity of about 99 percent by washing, dehydration and roasting. When the method is used for recovering the mixed rare earth chlorides and valuable elements such as iron and cobalt from the neodymium iron boron permanent magnet material scraps, the varieties and using amount of chemical raw materials can be reduced in a recovery process, the discharge of waste gases and water can be reduced, and the waste gases can be absorbed by ammonia water so as to be reused.
Owner:SHENYANG POLYTECHNIC UNIV
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