47 results about "Gene type" patented technology

The invention provides a molecular marker for detecting pepper light mottle virus L-series resistance genes and application of the molecular marker, and belongs to the technical field of genetic engineering, the molecular marker comprises SNP1 and SNP2, the SNP1 is a base T at the 2574th site of a yellow lantern pepper genome sequence with the NCBI login number of AB523370, and the SNP2 is a base T at the 2832nd site of the yellow lantern pepper genome sequence with the NCBI login number of AB523370; the specific gene sequence is a specific gene sequence of an L-series resistance gene L1c type gene, and the specific gene sequence is a basic group CA at the 2872 site to the 2873 site, a basic group TT at the 2877 site to the 2878 site, a basic group TG at the 2882 site to the 2883 site and a basic group AGA at the 2885 site to the 2887 site of the yellow lantern pepper with the NCBI accession number being AB523374. By utilizing the molecular marker and the specific sequence, the L-series resistance gene types of the yellow lantern pepper and the annual pepper can be rapidly and accurately detected, and the molecular marker can be used for molecular marker-assisted selective breeding.

The invention provides a breeding method for constructing XX / XY sex determination all-female sterile fish and application. The method comprises the following steps: knocking out a catalytic enzyme coding gene of a fish sex steroid hormone synthesis pathway by using a gene editing technology to block the fish sex steroid hormone synthesis pathway, screening to obtain an effectively mutated homozygote XX sex genetic type pseudo male fish, and hybridizing the pseudo male fish with improved allotetraploid crucian and carp female fish to obtain the fish sex steroid hormone synthesis pathway. Therefore, an allotriploid population is obtained, and the population completely develops into female fishes and is sterile, so that the effect of obtaining all-female and sterile populations is achieved at the same time. The method provided by the invention has very strong applicability, and can be widely applied to aquaculture of fishes to obtain female unisexual groups of the fishes so as to improve the aquaculture yield, realize effective control on female fertility and protect ecological safety; the method has a wide application prospect in the fields of genetic breeding and ecological safety of aquaculture fishes.

The invention relates to the field of plant genetic engineering, in particular to a rice OsDNG1 protein, a coding gene and application of the rice OsDNG1 protein to regulation and control of grain shape and grain weight. According to the invention, the OsDNG1 gene is knocked out through a CRISPR / Cas9 gene editing technology, so that the length of rice grains can be increased by 4.10%-4.62%, and the thousand grain weight can be increased by 10.86%-11.49%. And if the gene is over-expressed, the grain length is reduced by 1.14%-1.32%, and the thousand grain weight is reduced by 8.24%-8.56%. In the re-sequenced indica rice variety, the indica rice variety without the OsDNG1 gene type is longer in grain length and larger in length-width ratio compared with the variety with the gene type. The invention not only provides a key target for analyzing the molecular mechanism of rice grain morphogenesis, but also provides important theoretical basis and technical support for molecular design breeding of rice grain shape and grain weight, and has remarkable agricultural application value.

The invention discloses a japonica rice amphiphilic hybridization target gene plant screening method based on high-throughput sequencing, and belongs to the technical field of agricultural biology. A high-throughput sequencing technology is utilized, the genetic difference of parent genomes is accurately analyzed, specific molecular markers covering target gene areas are developed, plants containing target genes can be rapidly recognized, the screening efficiency and accuracy are greatly improved, the breeding period is shortened, resources are saved, and the method is suitable for large-scale popularization and application. Through combination of molecular marker detection and phenotypic expression of target characters, in the construction process of hybridization and backcross populations, target genes are fixed generation by generation, stable inheritance of the target characters, joint analysis of genetic types and phenotypes and multi-environment testing are ensured, correlation between target genotypes and the target characters and environmental adaptability of the target genotypes and the target characters are verified, and the target genotypes and the target characters are identified. The stability and reliability of the breeding material are improved, in candidate plant screening and restorer line creation, an excellent hybrid combination is constructed, genotype optimization is realized, and the quality and yield potential of the breeding material are improved.

The invention discloses a method for constructing gene-free edited all-male fish based on a kdm6bb gene knock-down technology, which comprises the following steps: carrying out knock-down treatment on a fish kdm6bb gene by utilizing a CRISPR / Cas9 gene editing technology, and carrying out genotype identification to obtain a kdm6bb heterozygote (kdm6bb + / -); a kdm6bb heterozygous mutant capable of effectively reducing kdm6bb protein expression is obtained through selfing of heterozygous fish; pseudo female fishes with XY sex genetic types are screened through sex molecular markers, the pseudo female fishes are mated with wild XY male fishes, YY sex genetic super-male fishes without kdm6bb mutation are screened, and the YY sex genetic super-male fishes are hybridized with wild XX female fishes, so that all-male fish groups without kdm6bb mutation are obtained, unisexual culture of fishes is realized, sex control breeding efficiency is improved, and breeding cost is reduced. The present invention is widely applicable to aquatic economic fishes on the basis of XX / XY sex determination.

A SNP molecular marker combination for identifying an AA broiler includes 25 SNP molecular markers, SNP sites of the SNP molecular markers 1 to 25 are located at a 51st position of each of nucleotide sequences shown in SEQ ID NO: 1 to 25. The SNP molecular marker combination for identifying the AA broiler in the disclosure has apparent species specificity of the AA broiler, is a unique mutation and importance determining site for the AA broiler, and can quickly identify authenticity of the AA broiler with less genotype information, providing new technical references for the identification, conservation, and genetic breeding of chicken breeds in the future.

The invention discloses a gene detection system which comprises a sample collection module, a gene extraction module, a sequencing module, a data analysis module and a report generation module. The gene extraction module is used for extracting high-quality DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) from a sample, and the data analysis module is used for processing, analyzing and explaining original data obtained by sequencing by integrating various bioinformatics algorithms and tools. The game theory is integrated into the whole genome association analysis GWAS model to improve the whole genome association analysis GWAS model, the game theory is combined to enable the SNP sites to be more and more detailed, the relevance between deeper hereditary characters and defective genes can be mined, the false positive probability of the result obtained by the association model is smaller, and the accuracy of the result obtained by the association model is improved. A genetic-defect gene association map is constructed according to a whole genome association analysis GWAS model fused with the game theory thought, a defect gene risk prediction model is trained, and the most possible defect gene type is predicted according to genetic characteristics, so that the accuracy is greatly improved.

The invention discloses wheat thousand seed weight related SNP-A1473G and application thereof, an SNP site corresponds to the 1473rd base from the 5'tail end of a sequence shown in SEQ ID NO: 1, and when the site is AA homozygous, the corresponding gene type I is shown; when the locus is GG homozygous, the corresponding locus is a genotype II; the thousand seed weight of the wheat with the homozygous genotype I is greater than or candidate to be greater than that of the wheat with the homozygous genotype II. The SNP has high effectiveness and potential application value, wheat with high thousand seed weight can be found by detecting the SNP, and the SNP has important value in research or application of cultivation of high-yield wheat varieties.

The application discloses a kind of based on molecular marker clustering analysis's cotton hybrid breeding combination screening method, the screening method includes: step S1: using multi-traits related molecular marker to the gene typing experiment of test cotton variety, data collection and arrangement are carried out;Step S2: excellent allele frequency calculation and feature matrix construction;Step S3: data standardization and principal component analysis dimension reduction;Step S4: optimal cluster number selection and genetic clustering;Step S5: each population character analysis;Step S6: heterosis potential score and combination screening.The technical problem that present cotton breeding parent selection lacks multi-traits comprehensive quantitative evaluation is solved, and the data of breeding decision-making and intelligentization are realized.

The invention discloses a quadruple real-time fluorescent quantitative PCR (Polymerase Chain Reaction) primer probe combination for detecting African swine fever virus and application. The primer probe combination comprises a first primer probe combination, a second primer probe combination, a third primer probe combination and a fourth first primer probe combination, the primer probe is capable of specifically targeting ASFV p72 gene, CD2v-I gene, CD2v-II gene and I177L gene, and the detection kit developed based on the primer probe combination can rapidly diagnose African swine fever virus and identify CD2v gene I and II type and CD2v and I177L gene deleted strains. The kit has extremely high sensitivity, the lowest detection limit can reach 8 copies / mu L, it is ensured that trace virus DNA can be detected, and missing detection is avoided.

The invention belongs to the technical field of biology, and particularly relates to a genotyping dot matrix chip for detecting African swine fever viruses as well as a construction method and application of the genotyping dot matrix chip. Firstly, the invention provides a single nucleotide polymorphism site combination for accurately detecting African swine fever gene type I, type II and I / II recombinant viruses; secondly, the invention provides a genotyping dot matrix chip for detecting the African swine fever virus, and the chip can simultaneously realize accurate typing, epidemic and variation monitoring and I / II recombinant virus recombinant gene identification of a high-throughput detection sample.

The application discloses a method and a kit for constructing a high-throughput multiplex fluorescence PCR-based HLA-CREG and HPA gene typing synchronous detection system. The application is based on common specific SNP sites, and two types of different primers are combined and designed to obtain a system for HLA cross-reactive group and HPA gene typing detection, which can realize rapid, simple, low-cost and high-throughput HLA epitope and HPA gene typing detection, so as to more effectively prevent platelet transfusion invalidity and immunological platelet transfusion invalidity.

The invention relates to genetic law simulation prediction, in particular to a construction and training method of a genetic law simulation prediction model, which comprises the following steps: taking a gene type as a hidden state of a hidden Markov model, and taking a gene phenotype as an observation value of the hidden Markov model; determining initial state probability distribution of the hidden Markov model; constructing a state transition probability matrix of the hidden Markov model based on a diagonal dominant matrix method; determining an observation probability distribution matrix of the hidden Markov model; taking the obtained hidden Markov model as a genetic law simulation prediction model; according to the technical scheme provided by the invention, the defect that the genetic law cannot be efficiently and quickly simulated and predicted in the prior art can be effectively overcome.

The application discloses a breeding method of Yunnan fragrant soft rice, which comprises the following steps: step one is to excavate specific rice resources, step two is to create new germplasm backbone parents, step three is to perform 'two-way' separation expression and single recessive relative trait elimination, and step four is to perform excellent trait balanced selection. In the application, indica rice or semi-indica rice resources from 1450-1650m warm and cool rice areas in Yunnan are collected, apparent morphological identification is performed on the resources and low-heat valley rice area materials, excellent materials with complementary traits are screened, and fragrant soft rice breeding intermediate materials and backbone parent reserves are created. In addition, parent populations with far genetic distance and different ecotypes are created, excellent genes with far genetic distance are introduced into Yunnan fragrant soft rice materials, according to the physiological characteristics of similar ecotype rice, affinity is improved, blood penetration and fusion are increased, backbone parents with rich and varied gene types are created, and'sticky + waxy' and 'colored vs. non-colored' parents are selected to perform hybridization, recessive trait materials are selected or eliminated, and the breeding efficiency can be improved.

The invention discloses an HLA gene typing detection primer group, a kit and a typing detection method.The primer group comprises primer pairs used for amplifying HLA-A, HLA-B, HLA-C, HLA-DRA, HLA-DRB1 / 3 / 4 / 5, HLA-DQA1, HLA-DQB1, HLA-DPA1 and HLA-DPB1 genes, specific amplification primer design is combined with nanopore sequencing of amplicons, complete haplotype information can be obtained, and typing accuracy and resolution are higher.

The invention discloses a reagent for detecting African swine fever virus gene type II and application of the reagent. The reagent comprises a primer group, the primer group comprises a forward primer with a sequence as shown in SEQ ID NO: 2 and a reverse primer with a sequence as shown in SEQ ID NO: 3. Compared with other similar kits, the kit for detecting the African swine fever gene type II, provided by the invention, has the advantages that the sensitivity can be remarkably improved through the design of the primers, the specificity is high, the sensitivity is high, the amplification efficiency is high, the detection time is short and the like; according to the kit provided by the invention, a user does not need to carry out reagent split charging, the extracted sample nucleic acid is directly added into the pre-split freeze-dried reagent to be detected in a fluorescent PCR instrument, and the operation is simple and rapid.

This invention provides a molecular marker for detecting L-series resistance genes to pepper mild mottle virus and its application, belonging to the field of genetic engineering technology. The molecular marker includes SNP1 and SNP2, where SNP1 is the T base at position 2574 of the gene sequence with NCBI accession number AB523370, and SNP2 is the T base at position 2832 of the gene sequence with NCBI accession number AB523370. The specific gene sequence is a specific gene sequence for the L1c type of the L-series resistance gene, specifically the CA bases at positions 2872-2873, the TT bases at positions 2877-2878, the TG bases at positions 2882-2883, and the AGA bases at positions 2885-2887 of the gene sequence with NCBI accession number AB523374. This invention utilizes the aforementioned molecular markers and specific sequences to rapidly and accurately detect L-series resistance gene types in yellow lantern peppers and annual peppers, and can be used for marker-assisted selection breeding.

The application belongs to the technical field of biology and particularly relates to a gene typing dot array chip for detecting African swine fever virus and a construction method and application thereof. The application firstly provides a single nucleotide polymorphism site combination for precisely detecting African swine fever virus of genotype I, genotype II and I / II recombinant virus. Secondly, the application provides a gene typing dot array chip for detecting African swine fever virus, which can simultaneously realize precise typing, epidemic and variation monitoring and identification of the recombinant gene of I / II recombinant virus of high-throughput detection samples.

The invention discloses a fusion protein of an anti-gene type 2 PRRSV (Porcine Reproductive and Respiratory Syndrome Virus) bivalent nano antibody and horse radish peroxidase (HRP) as well as a preparation method and application thereof, and belongs to the technical field of biology. The invention also evaluates the application of the fusion protein in detecting the anti-gene type 2 PRRSV antibody in porcine serum. The anti-gene type 2 PRRSV bivalent nano antibody and the HRP fusion protein are used as competitive reagents to be applied to competitive ELISA for detecting the anti-gene type 2 PRRSV antibody in porcine serum, the detection method is simple to operate, short in sample detection time, free of use of an enzyme-labeled secondary antibody and low in production cost, and the coincidence rate with an existing IDEXX PRRSV antibody detection commercialized kit is as high as 96.92%. The sensitivity is higher than that of ELISA (Enzyme-Linked Immuno Sorbent Assay) taking a monovalent nano antibody as a competitive reagent, and the competitive rate with positive serum can be increased by about 20%. Therefore, the subsequent development of the anti-PRRSV antibody ELISA detection kit by using the divalent nano antibody has a good market application prospect.

The invention belongs to the field of biological detection, and particularly relates to a fluorescent quantitative PCR (polymerase chain reaction) detection method of KIR2DS5 mRNA (messenger ribonucleic acid) as well as a detection kit, a primer and a design method of a probe. The method comprises the following steps: (1) performing KIR genetic typing on a DNA (deoxyribonucleic acid) sample by using a PCR-SSOP method, and screening out KIR2DS5 positive DNA; (2) carrying out PCR (Polymerase Chain Reaction) amplification and electrophoresis on the positive DNA sample; (3) extracting a KIR2DS5 specific DNA band, tapping rubber, purifying, connecting with a T vector, transforming, selecting flora, extracting plasmids, and sequencing; (4) comparing a sequencing result with a KIR / IPD database to confirm that the sequence is a characteristic gene sequence; and (5) designing a primer and a probe by using primer design software according to the characteristic KIR2DS5 gene sequence. And carrying out gene detection by using the designed primer and probe. According to the method, the specificity and accuracy of PCR detection are improved.

We will develop a probe set that can process multiple samples simultaneously using sequence capture methods and comprehensively capture genes in the KIR region. [Solution] A probe set for KIR gene typing is provided, each consisting of oligonucleotides having a specific base sequence. Furthermore, a method for KIR gene typing using the probe set is provided.

The invention discloses a gene for expressing Newcastle disease virus LaSota strain fusion (F) protein and application of the gene. Specifically, the invention discloses a nucleotide molecule for expressing the gene type II Newcastle disease virus F protein, and the nucleotide molecule has a sequence as shown in SEQ ID NO: 1; the invention also provides a recombinant expression plasmid containing the nucleotide molecule, and a DNA vaccine containing the recombinant expression plasmid. The DNA vaccine has a good immune protection effect on Newcastle disease gene IX type standard virulent F48E9 and current epidemic gene VII type virulent HeB38, and the detoxification level of a host subject is remarkably reduced.

The invention discloses application of a KASP molecular marker in identification of red peel / yellow peel characters of muskmelon, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on the red peel / yellow peel character of the muskmelon. The invention relates to a method for identifying red peel / yellow peel characters of muskmelons, which comprises the following steps: extracting the genome DNA of a target muskmelon, carrying out PCR amplification by taking the genome DNA of the target muskmelon as a template, and carrying out fluorescence detection based on a primer pair of a KASP molecular marker RY-1 or RY-2 to obtain the gene type of the target muskmelon, on the basis of the detected gene type, judging the character of the target muskmelon; wherein the KASP molecular marker RY-1 and the KASP molecular marker RY-2 are respectively designed according to SNP (Single Nucleotide Polymorphism) loci at the position of Chr04: 469552 and the position of Chr04: 760234. The identification method provided by the invention can be more efficiently applied to the fields of muskmelon molecular marker-assisted breeding, variety purity detection, gene editing target screening and the like.

This invention provides a highly practical probe set and a method for typing HLA genes using it, which enable efficient and highly accurate simultaneous typing of a large number of samples using the sequence capture method. [Solution] A probe set for HLA gene typing is provided, each consisting of oligonucleotides having a specific base sequence. Furthermore, an HLA gene typing method using the sequence capture method with the probe set is provided.

The application discloses a multiplex PCR detection system, a kit and application for RH gene typing detection. The application can efficiently and accurately identify common Rh genotypes in Asian population through a single PCR amplification reaction. The application not only effectively makes up for the limitations of traditional serological methods in detecting complex blood types such as D variant, but also has the advantages of high standardization, low cost, simple operation and low clinical promotion threshold.

The invention belongs to the technical field of statins, and particularly relates to a gene detection kit and system for evaluating the medication of statins, the kit comprises primers and probes for detecting the rs4149056 site of an SLCO1B1 gene, the rs2231142 site of an ABCG2 gene and the rs1057910 site of a CYP2C9 gene, the kit also comprises a PCR reaction liquid, a positive quality control product and a negative quality control product, the system comprises a PCR reaction system, a PCR amplification system and a genotyping interpretation system, the PCR reaction system is composed of the gene detection kit for evaluating the medication of statins, the kit and the detection system have the advantages of being high in sensitivity, low in cost, high in specificity, easy and convenient to operate, short in detection period and the like, and individual SLCO1B1, ABCG2 and CYP2C9 gene polymorphism can be rapidly and accurately detected.

The application discloses a japonica rice parent hybrid target gene plant screening method based on high-throughput sequencing and belongs to the field of agricultural biotechnology. The application uses high-throughput sequencing technology to accurately analyze the genetic differences of parent genomes, develops specific molecular markers covering target gene regions, can quickly identify plants containing target genes, greatly improves the screening efficiency and accuracy, shortens the breeding cycle and saves resources, combines molecular marker detection with the phenotypic performance of target traits, fixes target genes in each generation during the construction of hybrid and backcross populations, ensures the stable inheritance of target traits, and verifies the correlation between target genotypes and target traits and their environmental adaptability through joint analysis of genotypes and phenotypes and multi-environment testing, improves the stability and reliability of breeding materials, constructs excellent hybrid combinations and realizes genotype optimization in candidate plant screening and restorer line creation, and improves the quality and yield potential of breeding materials.

The application discloses a method for constructing gene editing-free all-male fish based on kdm6bb gene knockdown technology, comprising the following steps: using CRISPR / Cas9 gene editing technology to perform knockdown treatment on fish kdm6bb genes, and obtaining kdm6bb heterozygotes (kdm6bb+ / -) through genotyping; obtaining kdm6bb heterozygous mutants with effectively reduced kdm6bb protein expression through self-crossing of the heterozygote fish; screening pseudo-female fish with XY gender genetic type through gender molecular markers, mating with wild-type XY male fish, screening YY gender genetic type super-male fish without kdm6bb mutation, crossing with wild-type XX female fish, obtaining all-male fish population without carrying kdm6bb mutation, realizing monosex culture of fish, improving the efficiency of gender control breeding, and showing wide adaptability in aquatic economic fish based on XX / XY gender determination.

The invention relates to the technical field of biological information, in particular to an effective anti-disease gene prediction method based on different rice area rice blast flora. According to the method, different varieties of rice plants in different rice areas are analyzed, and the expansion infection ability is analyzed according to the infection condition of strains containing different abnormal non-toxic gene types; analyzing the disease expansion degrees of the strains corresponding to all the abnormally non-toxic genes to the rice regions and the growth adaptability of the strains in different rice regions by combining historical data to determine the damage ability of the strains, and analyzing the damage durability of the strains based on the genetic relationship of the rice plants corresponding to different strains. And according to the obtained damage durability degree, adjusting the attention weight of the node corresponding to each strain to obtain graph neural network prediction. In rice plants infected with rice blast in different rice areas, the damage degree of each bacterial strain to rice is analyzed, the effective resistance of different varieties of rice plants to the bacterial strain is predicted according to the stable expansion capability of the bacterial strain, and the prediction precision of the effectiveness of the disease-resistant gene is improved.

The invention relates to a probe, a primer and a kit for identifying gene I type, gene II type and gene I / II recombinant African swine fever viruses. Probes for identifying gene I type, gene II type and gene I / II recombinant African swine fever viruses comprise SNP (Single Nucleotide Polymorphism) typing probes P1 and P2 aiming at gene I type and gene II type ASFV B646L genes and a specific probe P3 aiming at gene II type ASFV CP204L genes, and nucleotide sequences of the P1, the P2 and the P3 are as shown in SEQ ID NO.5-7. The application not only can effectively distinguish the gene I type ASFV from the gene II type ASFV, but also can identify the gene I / II type recombinant African swine fever virus currently popular in China.