29 results about "Gene type" patented technology

The invention discloses wheat thousand seed weight related SNP-A1473G and application thereof, an SNP site corresponds to the 1473rd base from the 5'tail end of a sequence shown in SEQ ID NO: 1, and when the site is AA homozygous, the corresponding gene type I is shown; when the locus is GG homozygous, the corresponding locus is a genotype II; the thousand seed weight of the wheat with the homozygous genotype I is greater than or candidate to be greater than that of the wheat with the homozygous genotype II. The SNP has high effectiveness and potential application value, wheat with high thousand seed weight can be found by detecting the SNP, and the SNP has important value in research or application of cultivation of high-yield wheat varieties.

The application discloses a kind of based on molecular marker clustering analysis's cotton hybrid breeding combination screening method, the screening method includes: step S1: using multi-traits related molecular marker to the gene typing experiment of test cotton variety, data collection and arrangement are carried out;Step S2: excellent allele frequency calculation and feature matrix construction;Step S3: data standardization and principal component analysis dimension reduction;Step S4: optimal cluster number selection and genetic clustering;Step S5: each population character analysis;Step S6: heterosis potential score and combination screening.The technical problem that present cotton breeding parent selection lacks multi-traits comprehensive quantitative evaluation is solved, and the data of breeding decision-making and intelligentization are realized.

The invention belongs to the technical field of biology, and particularly relates to a genotyping dot matrix chip for detecting African swine fever viruses as well as a construction method and application of the genotyping dot matrix chip. Firstly, the invention provides a single nucleotide polymorphism site combination for accurately detecting African swine fever gene type I, type II and I / II recombinant viruses; secondly, the invention provides a genotyping dot matrix chip for detecting the African swine fever virus, and the chip can simultaneously realize accurate typing, epidemic and variation monitoring and I / II recombinant virus recombinant gene identification of a high-throughput detection sample.

The application discloses a method and a kit for constructing a high-throughput multiplex fluorescence PCR-based HLA-CREG and HPA gene typing synchronous detection system. The application is based on common specific SNP sites, and two types of different primers are combined and designed to obtain a system for HLA cross-reactive group and HPA gene typing detection, which can realize rapid, simple, low-cost and high-throughput HLA epitope and HPA gene typing detection, so as to more effectively prevent platelet transfusion invalidity and immunological platelet transfusion invalidity.

The invention relates to genetic law simulation prediction, in particular to a construction and training method of a genetic law simulation prediction model, which comprises the following steps: taking a gene type as a hidden state of a hidden Markov model, and taking a gene phenotype as an observation value of the hidden Markov model; determining initial state probability distribution of the hidden Markov model; constructing a state transition probability matrix of the hidden Markov model based on a diagonal dominant matrix method; determining an observation probability distribution matrix of the hidden Markov model; taking the obtained hidden Markov model as a genetic law simulation prediction model; according to the technical scheme provided by the invention, the defect that the genetic law cannot be efficiently and quickly simulated and predicted in the prior art can be effectively overcome.

The application discloses a breeding method of Yunnan fragrant soft rice, which comprises the following steps: step one is to excavate specific rice resources, step two is to create new germplasm backbone parents, step three is to perform 'two-way' separation expression and single recessive relative trait elimination, and step four is to perform excellent trait balanced selection. In the application, indica rice or semi-indica rice resources from 1450-1650m warm and cool rice areas in Yunnan are collected, apparent morphological identification is performed on the resources and low-heat valley rice area materials, excellent materials with complementary traits are screened, and fragrant soft rice breeding intermediate materials and backbone parent reserves are created. In addition, parent populations with far genetic distance and different ecotypes are created, excellent genes with far genetic distance are introduced into Yunnan fragrant soft rice materials, according to the physiological characteristics of similar ecotype rice, affinity is improved, blood penetration and fusion are increased, backbone parents with rich and varied gene types are created, and'sticky + waxy' and 'colored vs. non-colored' parents are selected to perform hybridization, recessive trait materials are selected or eliminated, and the breeding efficiency can be improved.

This invention provides a molecular marker for detecting L-series resistance genes to pepper mild mottle virus and its application, belonging to the field of genetic engineering technology. The molecular marker includes SNP1 and SNP2, where SNP1 is the T base at position 2574 of the gene sequence with NCBI accession number AB523370, and SNP2 is the T base at position 2832 of the gene sequence with NCBI accession number AB523370. The specific gene sequence is a specific gene sequence for the L1c type of the L-series resistance gene, specifically the CA bases at positions 2872-2873, the TT bases at positions 2877-2878, the TG bases at positions 2882-2883, and the AGA bases at positions 2885-2887 of the gene sequence with NCBI accession number AB523374. This invention utilizes the aforementioned molecular markers and specific sequences to rapidly and accurately detect L-series resistance gene types in yellow lantern peppers and annual peppers, and can be used for marker-assisted selection breeding.

The application belongs to the technical field of biology and particularly relates to a gene typing dot array chip for detecting African swine fever virus and a construction method and application thereof. The application firstly provides a single nucleotide polymorphism site combination for precisely detecting African swine fever virus of genotype I, genotype II and I / II recombinant virus. Secondly, the application provides a gene typing dot array chip for detecting African swine fever virus, which can simultaneously realize precise typing, epidemic and variation monitoring and identification of the recombinant gene of I / II recombinant virus of high-throughput detection samples.

The invention belongs to the field of biological detection, and particularly relates to a fluorescent quantitative PCR (polymerase chain reaction) detection method of KIR2DS5 mRNA (messenger ribonucleic acid) as well as a detection kit, a primer and a design method of a probe. The method comprises the following steps: (1) performing KIR genetic typing on a DNA (deoxyribonucleic acid) sample by using a PCR-SSOP method, and screening out KIR2DS5 positive DNA; (2) carrying out PCR (Polymerase Chain Reaction) amplification and electrophoresis on the positive DNA sample; (3) extracting a KIR2DS5 specific DNA band, tapping rubber, purifying, connecting with a T vector, transforming, selecting flora, extracting plasmids, and sequencing; (4) comparing a sequencing result with a KIR / IPD database to confirm that the sequence is a characteristic gene sequence; and (5) designing a primer and a probe by using primer design software according to the characteristic KIR2DS5 gene sequence. And carrying out gene detection by using the designed primer and probe. According to the method, the specificity and accuracy of PCR detection are improved.

We will develop a probe set that can process multiple samples simultaneously using sequence capture methods and comprehensively capture genes in the KIR region. [Solution] A probe set for KIR gene typing is provided, each consisting of oligonucleotides having a specific base sequence. Furthermore, a method for KIR gene typing using the probe set is provided.

The invention discloses application of a KASP molecular marker in identification of red peel / yellow peel characters of muskmelon, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on the red peel / yellow peel character of the muskmelon. The invention relates to a method for identifying red peel / yellow peel characters of muskmelons, which comprises the following steps: extracting the genome DNA of a target muskmelon, carrying out PCR amplification by taking the genome DNA of the target muskmelon as a template, and carrying out fluorescence detection based on a primer pair of a KASP molecular marker RY-1 or RY-2 to obtain the gene type of the target muskmelon, on the basis of the detected gene type, judging the character of the target muskmelon; wherein the KASP molecular marker RY-1 and the KASP molecular marker RY-2 are respectively designed according to SNP (Single Nucleotide Polymorphism) loci at the position of Chr04: 469552 and the position of Chr04: 760234. The identification method provided by the invention can be more efficiently applied to the fields of muskmelon molecular marker-assisted breeding, variety purity detection, gene editing target screening and the like.

This invention provides a highly practical probe set and a method for typing HLA genes using it, which enable efficient and highly accurate simultaneous typing of a large number of samples using the sequence capture method. [Solution] A probe set for HLA gene typing is provided, each consisting of oligonucleotides having a specific base sequence. Furthermore, an HLA gene typing method using the sequence capture method with the probe set is provided.

The application discloses a multiplex PCR detection system, a kit and application for RH gene typing detection. The application can efficiently and accurately identify common Rh genotypes in Asian population through a single PCR amplification reaction. The application not only effectively makes up for the limitations of traditional serological methods in detecting complex blood types such as D variant, but also has the advantages of high standardization, low cost, simple operation and low clinical promotion threshold.

The invention belongs to the technical field of statins, and particularly relates to a gene detection kit and system for evaluating the medication of statins, the kit comprises primers and probes for detecting the rs4149056 site of an SLCO1B1 gene, the rs2231142 site of an ABCG2 gene and the rs1057910 site of a CYP2C9 gene, the kit also comprises a PCR reaction liquid, a positive quality control product and a negative quality control product, the system comprises a PCR reaction system, a PCR amplification system and a genotyping interpretation system, the PCR reaction system is composed of the gene detection kit for evaluating the medication of statins, the kit and the detection system have the advantages of being high in sensitivity, low in cost, high in specificity, easy and convenient to operate, short in detection period and the like, and individual SLCO1B1, ABCG2 and CYP2C9 gene polymorphism can be rapidly and accurately detected.

The application discloses a japonica rice parent hybrid target gene plant screening method based on high-throughput sequencing and belongs to the field of agricultural biotechnology. The application uses high-throughput sequencing technology to accurately analyze the genetic differences of parent genomes, develops specific molecular markers covering target gene regions, can quickly identify plants containing target genes, greatly improves the screening efficiency and accuracy, shortens the breeding cycle and saves resources, combines molecular marker detection with the phenotypic performance of target traits, fixes target genes in each generation during the construction of hybrid and backcross populations, ensures the stable inheritance of target traits, and verifies the correlation between target genotypes and target traits and their environmental adaptability through joint analysis of genotypes and phenotypes and multi-environment testing, improves the stability and reliability of breeding materials, constructs excellent hybrid combinations and realizes genotype optimization in candidate plant screening and restorer line creation, and improves the quality and yield potential of breeding materials.

The application discloses a method for constructing gene editing-free all-male fish based on kdm6bb gene knockdown technology, comprising the following steps: using CRISPR / Cas9 gene editing technology to perform knockdown treatment on fish kdm6bb genes, and obtaining kdm6bb heterozygotes (kdm6bb+ / -) through genotyping; obtaining kdm6bb heterozygous mutants with effectively reduced kdm6bb protein expression through self-crossing of the heterozygote fish; screening pseudo-female fish with XY gender genetic type through gender molecular markers, mating with wild-type XY male fish, screening YY gender genetic type super-male fish without kdm6bb mutation, crossing with wild-type XX female fish, obtaining all-male fish population without carrying kdm6bb mutation, realizing monosex culture of fish, improving the efficiency of gender control breeding, and showing wide adaptability in aquatic economic fish based on XX / XY gender determination.

The invention relates to the technical field of biological information, in particular to an effective anti-disease gene prediction method based on different rice area rice blast flora. According to the method, different varieties of rice plants in different rice areas are analyzed, and the expansion infection ability is analyzed according to the infection condition of strains containing different abnormal non-toxic gene types; analyzing the disease expansion degrees of the strains corresponding to all the abnormally non-toxic genes to the rice regions and the growth adaptability of the strains in different rice regions by combining historical data to determine the damage ability of the strains, and analyzing the damage durability of the strains based on the genetic relationship of the rice plants corresponding to different strains. And according to the obtained damage durability degree, adjusting the attention weight of the node corresponding to each strain to obtain graph neural network prediction. In rice plants infected with rice blast in different rice areas, the damage degree of each bacterial strain to rice is analyzed, the effective resistance of different varieties of rice plants to the bacterial strain is predicted according to the stable expansion capability of the bacterial strain, and the prediction precision of the effectiveness of the disease-resistant gene is improved.

The invention discloses a digital PCR (Polymerase Chain Reaction) detection kit for human NPM1 gene mutation. The kit at least comprises ddPCR reaction liquid and ddPCR MIX3, the ddPCR reaction liquid comprises primers and a probe, the primers comprise a mutation NPM1 forward primer and a mutation NPM1 reverse primer, the mutation NPM1 reverse primer comprises seven primers of NPM1 I-NPM1 VII type, the base sequence of the mutation NPM1 forward primer is SEQ ID No.1, and the base sequences of the seven primers of NPM1 I-NPM1 VII type are SEQ ID No.2-SEQ ID No.8. The kit is characterized in that the kit comprises a kit body, the kit body and the probe, and the kit body comprises a kit body and a kit body, the kit is used for qualitatively detecting NPM1 gene I-VII type mutation of bone marrow and peripheral blood samples of clinical AML patients, and can assist clinicians to carry out prognosis risk assessment, differential diagnosis, treatment guidance and AML typing judgment on the AML patients.

The application discloses a single-cell mixed sample sequencing and sample splitting method based on HLA genes. The application first acquires HLA gene typing information of each sample to be mixed; secondly, single-cell mixed sample sequencing is performed on the mixed sample to obtain single-cell mixed sample sequencing results of the mixed sample; finally, based on the HLA gene typing information, the sample source of each cell is identified by calculating the expression characteristics of HLA genes in the single-cell mixed sample sequencing sequence, and sample splitting is completed. The application breaks through the technical obstacle that few-cell clinical samples cannot be subjected to single-cell sequencing, greatly improves the utilization rate of these precious samples, significantly reduces the calculation consumption of sample splitting through an efficient HLA gene sequence pseudo-alignment algorithm, improves the ability to obtain genetic information from low-coverage cells by focusing on the HLA region with the highest polymorphism in the human genome, and effectively reduces the sequencing cost of a single sample through mixed sample sequencing.

The invention relates to the technical field of plant breeding, in particular to a molecular marker combination related to indica-japonica rice cross fertility and application of the molecular marker combination. The molecular marker combination comprises the following components: SaM-1235, SaF-6434K, Sc-1847, HSA1a-7551, F5-1005, DPL2-1347, Duyao-F2, Duyao-F60T, Jiyao-F2, Jiyao-I33L, Jiyao-K115E, Jiyao-K115Q, Jiyao-N114S, Jiyao-T474K, S5-9611, and S7-7581, and is characterized in that the molecular marker combination comprises the following components: Sc-1235, SaF-6434K, Sc-1847, HSA1a-7551, F5-1005, DPL2-1347, Duyao-F2 The molecular marker combination provided by the invention can be used for comprehensively and systematically detecting the type, quantity and combination of indica-japonica hybrid fertility genes carried by existing rice varieties and resources, and further can be applied to directional improvement of the hybrid fertility of the indica-japonica rice varieties.

The invention discloses a rice leaf width gene locus OsLwid9.13 as well as a molecular marker and application of the rice leaf width gene locus OsLwid9.13. The gene locus OsLwid9.13 is an SNP locus in a rice genome, the nucleotide variety of the gene locus OsLwid9.13 is T or C, the gene locus OsLwid9.13 is the 101th nucleotide of SEQ ID No.1 in a sequence table, and when the gene type of the OsLwid9.13 locus is CC, the rice has a leaf width or a candidate leaf width. A positioning group is prepared by utilizing a rice leaf width difference material, and a gene locus related to the leaf width is excavated and can be used for commercialized broad-leaf rice molecular breeding. The molecular marker-assisted selection efficiency is high, molecular marker-assisted selection can be carried out in the early breeding stage, the breeding cost is reduced, and the wide-leaf rice breeding process is accelerated.

The function of enhancing stem cells is accomplished with a specific transfected human gene type into a stem cell of choice to allow for the regenerative potential of the selected stem cell to be greatly enhanced by combining both gene and stem cell therapies together. Specific genes for selected protein production are cultured with a stem cell of choice which then produces a supernatant composed of an exosome-gene complex. This exosome-gene complex can also be used as a therapeutic gene therapy on its own or in combination with a stem cell gene complex together for a specific cellular effect, organ or tissue of regenerative effect or general systemic result.

The application relates to a primer probe set, a kit and a method for genotyping of a measles virus B3 / D8 / H1. The method for detecting the measles virus by using the primer probe set and the kit has good sensitivity, the sensitivity of the measles virus of different genotypes can reach 500 copies / mL, and the detection of low virus load can be realized.

The invention discloses a single cell mixed sample sequencing and sample splitting method based on an HLA (human leukocyte antigen) gene. The method comprises the following steps: firstly, acquiring HLA genotyping information of each to-be-mixed sample; secondly, mixing the to-be-mixed samples and then carrying out single-cell mixed sample sequencing to obtain a single-cell mixed sample sequencing result of the mixed samples; and finally, based on the HLA gene typing information, identifying the sample source of each cell by calculating the expression characteristics of the HLA gene in the single cell mixed sample sequencing sequence, and completing sequencing sample splitting. According to the method, the technical obstacle that single-cell sequencing cannot be carried out on a clinical sample with a small cell quantity is broken through, and the utilization rate of precious samples is greatly increased; the calculation consumption of sample splitting is obviously reduced through an efficient HLA gene sequence pseudo-alignment algorithm; by focusing on an HLA region with the highest polymorphism in a human genome, the capability of acquiring genetic information from low-coverage cells is improved; and the sequencing cost of a single sample is effectively reduced through mixed sample sequencing.

The invention belongs to the technical field of attention deficit hyperactivity disorder, and particularly relates to an attention deficit hyperactivity disorder medication guidance gene detection kit and detection system.The detection kit comprises a primer probe combination for detecting the rs1065852 site of a CYP2D6 gene, the rs4532 site of a DRD1 gene, the rs2283265 site of a DRD2 gene, the rs6280 site of a DRD3 gene and the rs2032582 site of an ABCB1 gene; the detection system comprises the kit, a PCR amplification system and a genotyping interpretation system. DNA extracted from a sample is added into a PCR reaction system composed of the kit, and PCR amplification is performed after uniform mixing; the attention deficit hyperactivity disorder medication guidance gene detection kit has the advantages of being high in sensitivity, low in cost, easy and convenient to operate, short in detection period, visual in interpretation and the like, and the attention deficit hyperactivity disorder related drug gene polymorphism can be rapidly and accurately detected.

The application discloses a corn southern rust resistance gene function marker, the gene is located on the 6th chromosome of corn, from the main QTL site RppQ6.01 of the southern rust resistance of Zi 319, gene silencing test shows that ZmWRKY66 is the disease resistance gene of RppQ6.01, sequence analysis finds that there is an insertion / deletion of CACTA-TIR transposon in the promoter region of ZmWRKY66, two function markers are developed based on the DNA sequence of the transposon, and are named as CACTA-L and CACTA-R. In the application, the main QTL and gene position of the corn southern rust resistance are clear, the phenotype contribution rate is higher, the southern rust resistance of corn inbred line material can be significantly improved, the development and application of the two function markers make the corn seedling stage ZmWRKY66 gene typing detection convenient and fast, and the application is not affected by the environment, and a feasible technical approach is provided for the molecular assisted breeding of the corn southern rust resistance.

The function of enhancing stem cells with a specific transfected human gene type into a stem cell of choice to allow for the regenerative potential of the selected stem cell to be greatly enhanced by combining both gene and stem cell therapies together. Specific genes for selected protein production are cultured with a stem cell of choice which then produces a supernatant composed of an exosome-gene complex. This exosome-gene complex can also be used as a therapeutic gene therapy on its own or in combination with a stem cell gene complex together for a specific cellular effect, organ or tissue of regenerative effect or general systemic result.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker associated with the milk fat percentage character of pig colostrum and application, the nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO: 1, an SNP site R is arranged at 531bp of the sequence of the SNP molecular marker, and R is A or G. Genetic typing is carried out by utilizing a gene chip technology, whole genome association analysis is carried out by combining the character of the milk fat percentage of the porcine colostrum, one SNP molecular marker obviously related to the character is screened out, and a new marker resource is provided for molecular breeding of the character of the component of the porcine colostrum. The kit disclosed by the invention has an important application prospect in boar breeding practice, is beneficial to improving the economic benefits of pig breeding enterprises, is also beneficial to promoting the development of the molecular breeding level of live pigs in China, and accelerates the breeding and popularization of excellent varieties.

This application discloses the application of KASP molecular markers in identifying the red / yellow skin trait in melons, belonging to the field of molecular marker technology. It aims to utilize molecular marker-assisted selection breeding for the red / yellow skin trait in melons to promote gene mapping and molecular-assisted breeding processes. A method for identifying the red / yellow skin trait in melons includes the following steps: extracting genomic DNA from the target melon; performing PCR amplification using the genomic DNA as a template; and performing fluorescence detection based on primer pairs of KASP molecular markers RY-1 or RY-2 to obtain the gene type of the target melon; and determining the trait of the target melon based on the detected gene type; wherein, KASP molecular markers RY-1 and RY-2 are designed based on SNP sites at Chr04:469552 and Chr04:760234, respectively. The identification method in this application can be more efficiently applied to fields such as molecular marker-assisted breeding of melons, variety purity detection, and gene editing target screening.