428 results about "Gene type" patented technology

Use of polymorphism site gene to predict ACEI medicine, its method and reagent kit are disclosed. It can be used to determine critical enzyme gene polymorphism site gene typing oligonucleotide and critical enzyme gene polymorphism site gene in cysteine metabolic pathway.

The invention discloses a method for predicating a homologous recombination deficiency (HRD) mechanism and a method for predicating response of patients to cancer therapy and relates to the field of biological information predication. The method comprises the step of judging whether a tumor sample has homologous recombination deficiency or not according to one or more comprehensive values in a large-segment INDEL (Insertion / Deletion) fraction, a copy number variation fraction and a tumor mutation load fraction, wherein the comprehensive values can also comprise a loss of heterozygosity variation fraction. By adopting the method disclosed by the invention, predication of a chromosome large-segment structure, a chromosome gene type number, a chromosome gene copy number, a chromosome variation interval and abnormal loss of heterozygosity and chromosome telomeric imbalance is realized, so that an evaluation range is more complete and HRD can be accurately predicated; the comprehensive values also can be used for determining whether the patients have response to a therapeutic regimen containing one or more of a PARP (Poly Adenosine Diphosphate Ribose Polymerase) inhibitor, an DNA (Deoxyribonucleic Acid) injury inhibitor, a topoisomerase II / II+inhibitor, a topoisomerase I inhibitor and radiotherapy; the method is simple and has wide general applicability.

A VII gene type of an attenuated strain of Newcastle disease virus A-NDV-VII and a construction method are disclosed. The invention relates to the application of reverse genetics technique. The invention uses the constructed reverse genetics platform of ZJ1 strain of Newcastle disease virus of goose origin. The invention replaces two envelope glycoprotein gene fragments F and HN of an isolated strain JS-5-05-Go of Newcastle disease virus with high reproductive performance with the corresponding fragments of the ZJ1 strain of Newcastle disease virus of goose origin, so that the recombinant virus NDV-VII is obtained. The VII gene type of Newcastle disease virus A-NDV-VII which is highly attenuated is rescued after the attenuated mutation of the F gene of the recombinant virus. And the virus has a higher reproduction titer on chicken embryo. The invention is suitable for a mass production of vaccine, which can be used for the manufacture of vaccine.

This invention relates to one polymer enzyme linkage reaction fluorescence test method to dialogue dangerous human body nipple shape virus and to isolate DNA sample HPV gene type to test one set of dangerous HPV infection from patient by the method, wherein, it belongs to life science and biological technique. This invention agent case comprises one fluorescence meter PCR technique as base of multi-layer polymer enzyme reaction composed of multiple HPV positive lead object, reaction lead object and fluorescence detector.

The invention discloses a method for building a standard CYP450 (cytochrome p450) gene type database. The method comprises the steps as follows: comparing a specific sequence corresponding to mutation information of a CYP450 gene type with a standard human whole genome sequence to obtain a corresponding relation between the specific sequence and the standard human whole genome sequence; and converting the CYP450 gene type into a gene type taking the standard human whole genome sequence as a reference sequence according to the corresponding relation. The invention further discloses a database built by the method and a CYP450 gene typing and enzymatic activity identification method based on the database. The database is convenient for research of CYP450; the gene typing method provides unified standards for CYP450 gene typing and provides more accurate judgment criterions for diseases, drugs or the like; unknown mutation sites in CYP450 genes can be effectively detected; hundreds of samples can be detected at the same time; and the coverage range is comprehensive and wide.

The invention relates to a nucleic acid cross flow test strip-based method for detecting single nucleotide polymorphism, comprising the following steps: firstly, preparing the nucleic acid cross flow test strip; secondly, obtaining a sample to be tested, denaturing and annealing; obtaining water, a nano-gold probe solutions, a connecting probe, Taq DNA ligase buffer solutions and Taq DNA ligase, and blending uniformly to obtain a mixed solution; adding KIF-1 and KIF-2 or the mixed solution of the two to the mixed solution, and blending uniformly; adding the sample to be tested, carrying out hybrid connection, denaturing, and annealing; and finally dropping obtained solutions on the binding area of the nucleic acid cross flow test strip, immersing the immersion area of the test strip into the running buffer solutions, and observing. The method achieves easy operation and low cost, is characterized by specificity, fastness as well as high resolution and sensitivity, and can be applied to the detection on the single nucleotide polymorphism and gene type as well as the identification on different pathogenic microorganisms of genes in hereditary diseases, communicable diseases, tumour and angiocardiopathy in clinical medicines.

The invention relates to a hereditable deafness susceptibility gene screen test kit. The kit uses mutation from C to T of 1,494 locus of a 12s rRNA gene, mutation from A to G of 1,555 locus of the 12srRNA gene, mutation from A to G of IVS7(-2) locus of an SLC26A4 gene and C(+ / -) of 235 locus of a GJB2 gene as detecting objects, designs and optimizes a set of specific primers respectively againsteach locus to be tested according to the PCR technical principle of a tetra-primer amplification refractory mutation system, amplifies the whole set of the primers in the same reaction tube, and performs primary multiple PCR amplification and primary gel electrophoresis on the four reaction tubes to obtain gene types of four loci simultaneously.

The invention relates to a method for identifying gene mutation types in the field of gene analysis as well as a special chip and a kit thereof. The gene mutation detecting method comprises the following steps: taking a genome to be detected from a human tissue as a template, carrying out multiple allele special PCR amplification by a primer group that is designed aiming at special mutant sites and DNA polymerase without 3'-5' end exonclease activity, then hybridizing the obtained PCR product and an oligonucleotide probe (allele special probe) on the gene chip, and confirming mutation types of all gene sites according to the hybridizing result. The allele special probe is designed aiming at special gene types of gene mutant sites to be detected. The invention can detect gene mutations in comprehensive, systemic and high-flux ways and has light environmental pollution as well as simple and rapid operation compared with PCR-RFLP and a sequencing method.

The invention relates to a molecule tagging method for a brown paddy plant hopper resistant master gene Bph3 of rice cultivars, which belongs to the technical field of genetic thremmatology. The brown paddy plant hopper resistant master gene Bph3 of an insect resistant variety Rathu Heenati is obtained by genetic linkage analysis of the brown paddy plant hopper resistant level of gene types of various single plants of F2 and various genealogies of F 2:3 obtained after hybridization of the insect resistant rice variety Rathu Heenati (female) and an insect susceptible variety 02428(male). The gene is positioned between a molecular marker A4 and a molecular marker RM16533, and the selection efficiency of three Indel indexes namely RH784, RH786 and RH007 of the interval is approximately 97 percent. Detection is made whether the insect resistant variety Rathu Heenati and derivative varieties (systems) of the insect resistant variety Rathu Heenati contain the master gene through the molecular markeres of the brown paddy plant hopper resistant master gene; the brown paddy plant hopper resistance level of the master gene can be predicted; and the selection efficiency of brown paddy plant hopper resistant rice can be greatly improved.

A system and method for determining the genetic data for one or a small set of cells, or from fragmentary DNA, where a limited quantity of genetic data is available, and also for predicting likely phenotypic outcomes using mathematical models and given genetic, phenotypic and / or clinical data of an individual, and also relevant aggregated medical data consisting of genotypic, phenotypic, and / or clinical data from germane patient subpopulations. Genetic data for the target individual is acquired and amplified using known methods, and poorly measured base pairs, missing alleles and missing regions are reconstructed using expected similarities between the target genome and the genome of genetically related subjects.

The invention relates to a method for performing multi-gene disease inheritance risk comprehensive assessment to an individual. The method comprises the following steps that: inheritance risk factors of a special multi-gene disease in a special crowd are screened; the risk degree of each risk factor to the special multi-gene disease is determined; and resources such as NCBI, HapMap databases and so on, are utilized to determine the frequencies of gene types of polymorphic gene type loci in the special crowd. The method is characterized in that: an assessment model is established, and the risk rank of the multi-gene disease of the individual is assessed from the genetic angle. The method is a comprehensive, objective, easily-operated, and intellectualized risk assessment method.

The invention discloses a human ALK fusion gene detection primer set and a detection kit. The primer set comprises one or more detection primers in sixteen ALK fusion gene types A01 to A16 and Taqman probes corresponding to the gene types. As many as sixteen fusion gene types except for EML4 can be detected through the detection kit formed by the primer set and the probes, the requirement for the sample RNA obtaining quality is low, and the ideal detection effect can be achieved through samples in different existence forms; by means of the kit, an operator only needs to directly feed extracted sample RNA once, inverse transcription and PCR amplification are carried out in one closed tube reaction system, and the pollution probability and the result error probability are reduced. One-time detection only takes eighty minutes. The human ALK fusion gene detection primer set and the detection kit have the remarkable advantages that the number of the detection types is large, sensitivity is high, experiment operation is simple, the period is short, the human ALK fusion gene detection primer set and the detection kit are safe and nontoxic, and cost is low.

The invention belongs to the field of molecular biology, and discloses a barcode preparation method used for individual animal identity identification and / or meat product tracing and application thereof. The method comprises the following steps of selecting single nucleotide polymorphism (SNP) sites with high heterozygosity on an animal to be identified or a meat product genome DNA to be traced and combining the SNP sites into an SNP barcode, and then using ten Arabic numbers 0-9 to randomly and uniquely replace the ten SNP gene types: A / A, T / T, G / G, C / C, A / T, A / G, A / C, T / G, T / C and G / C in the SNP barcode, forming corresponding numerical barcodes, and realizing correspondence of individual animal identities and barcodes one by one. According to the method provided by the invention, as a polymerase chain reaction (PCR) primer is designed, the SNP sites with high heterozygosity can be obtained, and the sites can be used for preparing the SNP barcode and / or corresponding numerical barcode used for identifying the individual animal identity and / or tracing the meat product, thereby realizing individual animal identity identification and meat product tracing.

The invention discloses an agent box for assessing pre-pregnant nutrition metabolism hereditary ability. The agent box comprises specificity primer pair and specificity fluorescent detecting probe pair for detecting synchronously number rs1801133 and rs1801131 SNP site on 5,10-methano tetrahydrofolic acid reducing ferment gene, number rs1801394 SNP site on methilanin synthetase reducing ferment gene, number rs731236 and rs1544410 on vitamin D receptor gene, number rs5443 SNP site on G albumen Beta3 second unit gene, general component for detecting fluorescent definite quantity PCR etc.. The agent box of the invention assesses pre-pregnant nutrition metabolism hereditary ability by detecting synchronously mononucleotide polymorphism site gene type correlative closely to pre-pregnant nutrition metabolism hereditary potence.

The invention provides an electrochemical DNA biosensor for detecting the BCR / ABL fusion gene of chronic myeloid leukemia (CML), which comprises the following steps of: (1) designing and synthesizing the specificity sequence of the CML according to the fusion locus of the universal primer sequence of the selected CML gene type to be detected; and (2) building the electrochemical DNA biosensor by combining a chemical bonding technology, a molecular hybridization technology, a locked nucleic acid probe technology with an electrochemical enzyme linked immunity technology for detecting the BCR / ABL fusion gene of the CML. The biosensor greatly strengthens the sensitivity and the specificity, thereby being capable of realizing the early diagnosis of the chronic myeloid leukemia (CML).

The invention discloses a method of detecting the quality of pork. In the method, the gene type of the pig is determined by detecting that the ribonucleotide is C or G on the 451st site on the 51end of the sequence 1 or the 112th site on the 5 (1) end of the sequence 2; and the quality of pork is determined by the gene type; the method of determining the gene type of the pig is as follows: when the ribonucleotide is C on the 451st site on the 5 (1) end of the sequence 1, the homozygote gene type is AA; when the ribonucleotide is G on the 112th site on the 51 end of the sequence 2, the homozygote gene type is BB; the heterozygous gene type is AB; the method of determining the quality of pork via the gene type is as follows: the AA gene type pork tenderness and pH are higher than the AB gene type pork; the AB gene type AB gene type are higher than the BB gene type pork. The method of invention can be applied to detect the tenderness and pH value of the pig which indicate the quality of muscle, which provides a method of accurately and conveniently detecting the hereditary character for the molecular breeding of pig.

The invention provides a molecular genetic marker related with an egg laying performance of hens and application. An FSH (Follicle-Stimulating Hormone)-beta gene is used as the molecular genetic marker related with the egg laying performance of the hens; the molecular genetic marker is characterized in that the nucleotide sequence of the molecular genetic marker is shown as SEQ ID No. 1 or the sequence at least comprises a specific fragment of a 259 T>G mutated basic group. The egg laying performance of the hens are selected according to gene types and the molecular genetic marker has the characteristics of high accuracy, simplicity in operation and low cost (the selection cost is about 0.3 yuan per hen) and the like; automatic detection can be carried out; secondly, a molecular marking method provided by the invention can be used for selecting properties of the egg laying performance of the hens at an early life stage (within one week after chickens are hatched), the generation interval can be shortened, the selection intensity is improved and high-quality breeding hen parents can be selected relatively early, so that a breeding progress of the hens is accelerated; compared with a conventional determination selection method for the egg laying performance of the hens, the selection time of each generation can be shortened by 16 months.

A method of using gene detection technique to provide active health service includes confirming gene polymorphism site carrier type of tested person by detecting phenotypic correlation gene polymorphism, confirming health genetic factor, forwarding personalized health guide and pharmacy report and gene typing detection report to tested person by using genetic factor as base and combining said base with investigation paper and body detection information of tested person.

The invention belongs to the technical field of biology, in particular to an HPA allelic gene typing detection reagent kit. The reagent kit of the invention comprises a primer and an MGB-probe. The reagent kit can judge the gene type of the allelic gene according to the observation of the difference of two kinds of fluorescence curves and the distribution of scattergrams after the amplification, so the HPA allelic gene typing detection can be completed. The reagent kit of the invention can meet the experiment requirement of high flux, and the whole typing experiment can be completed in two hours under the condition of the existence of proper DNA detection materials. The problem of fast systemic typing on HPA-1 to 5 and 15 on people groups in China can be solved, at the same time, the condition of homozygotes and heterozygotes can be identified, the typing results can be directly obtained, and the invention has the characteristics of flexibility, stability, accuracy and high efficiency.

The invention provides an electrochemical DNA biosensor for detecting PML / RAR alpha fusion gene of acute promyelocytic leukemia. The gene to be detected is the PML / RAR alpha fusion gene of the acute promyelocytic leukemia (APL); and the method comprises the following steps: (1) designing and synthesizing a specific sequence of the APL according to the selected fusion sites of a universal primer sequence of the gene type of the APL to be detected; and (2) constructing a nano modified electrode by a Au nano material, and constructing the nano electrochemical biosensor used for detecting the PML / RAR alpha fusion gene of the APL by the electrochemical enzyme-linked immunoassay technology. The method greatly improves the sensitivity of the biosensor so as to realize early diagnosis of the acute promyelocytic leukemia.

The invention belongs to the technical field of biology and particularly relates to a primer set and a kit for detecting rare deletion type thalassemia. The primer set and the kit can be directly used for rapidly and stably detecting domestic 11 types of known rare deletion type thalassemia. The primer set for detecting the rare deletion type thalassemia comprises a primer set A and a primer set B, wherein the primer set A comprises 12 primers for detecting alpha-thalassemia deletion gene types; the primer set B comprises 10 primers for detecting beta-thalassemia deletion gene types. The kit can adopt a multiplex Gap-PCR (Polymerase Chain Reaction) technology to detect 11 rare deletion gene types and can be used for directly detecting 11 rare deletion thalassemia gene types for one time. The kit is simple to operate and saves time and cost when being compared with a manner of combining a nested PCR with a genetic analysis in antenatal diagnosis; conditions are created for comprehensively carrying out thalassemia screening and scientific evidences are provided for thalassemia diagnosis of premarital detection, antenatal detection and fetuses of the pregnancy.

The invention belongs to the technical field of biological detection, and discloses HRM detecting primers and a method for distinguishing the foot-mouth disease virus (FMDV) and the Seneca Valley virus (SVV). The primers have the sequences shown in SEQ ID NO:1 and SEQ ID NO:2 and are high in specificity. By means of the primers, PCR amplification is conducted on the FMDV and the SVV, then fluorescent data is collected by monitoring the combination situation of double-chain DNA fluorescent dyes and PCR amplification products in the temperature rise process in real time, and the FMDV and the SVV are distinguished according to the difference of two dissolution curves; the two gene types can be distinguished after PCR amplification is conducted through the primers, it takes people only 3 hours for the whole operation process, no virus cell culture is needed, and the type distinguishing time is greatly shortened; expanses are low, no specific probe is needed, and fluorescent saturated dyes are low in price and easy to obtain; accuracy, specificity and repeatability are high, analysis can be accurately and rapidly conducted at high throughput, and the primers and method are easy to apply and popularize in clinical practices.

The invention discloses an apolipoprotein E4 ELISA kit and a preparation method thereof, the invention applies the purified human apolipoprotein E4 to prepare an anti-human ApoE4 antibody, and an ELISA plate is coated after the compatible purification. The kit composition includes the ELISA plate coated by the anti-human ApoE4 monoclonal antibody, an enzyme-labeled antibody, ApoE4 standard frozen powder, sample dilution solution, TMB substrate color developing solution, concentration washing liquid and reaction termination liquid. The ApoE4 in the antibody capture standard solution or the sample solution which is fixed on the microporous surface of the ELISA plate is identified by and combined with the enzyme-labeled antibody, so as to form the antibody-ApoE4-antibody-enzyme compound, the absorbance is measured after the reaction and color development of the enzyme and the substrate and the concentration of the ApoE4 in the sample can be calculated by the standard curve. The kit can detect the consistency of the apolipoprotein E4 in human serum, plasma or cerebrospinal fluid, the invention has the advantages of sensitivity, rapidness, simpleness and accuracy, so the invention provides the effective means for in vitro diagnosis and determination of ApoE4 gene type and the gene dosage.

The invention discloses a method for rapidly detecting expression of ANS (Anthocyanidin Synthetase) genes from different sources in rape seed capsules. In the method, a specific primer capable of distinguishing ANS genes from chromosome sets A, B and C by cloning and analyzing sequences of ANS genes in three elementary species of rape (cabbage: the chromosome set is AA, and 2n=20; brassica oleracea: the chromosome set is CC, and 2n=18; and brown mustard: the chromosome set is BB, and 2n=16) and three allotetraploids (mustard type rape: the chromosome set is AABBA, and 2n=36; cabbage type rape: the chromosome set is AACC, and 2n=38; brassica carinata: the chromosome set is BBCC, and 2n=34) according to the nucleotide polymorphic loci of ANS genes from the chromosome sets A, B and C, and the ANS gene types expressed in the seed capsules of rape of different chromosome set types are identified in combination with an RT-PCR (Reverse Transcriptase-Polymerase Chain Reaction). The method plays a role in further researching the molecular adjusting mechanism for the color formation of rapeseed capsules and accelerating the variety breeding of yellow seed rape; and meanwhile, a novel methodfor detecting the source of a rape ANS gene is provided.

The invention belongs to the fields of molecular biology and medicine, and relates to a method and a kit for detecting the polymorphic site of high blood pressure susceptibility gene. The invention provides the method for detecting the polymorphic site of the high blood pressure susceptibility gene, which comprises the following step of detecting the gene type of human mitofusin gene 2 (Mfn2) rs3820189 site, wherein the rs3820189 is provided with an individual of T gene type, and the susceptibility of the high blood pressure is obviously higher than common crowd. The invention further discloses a corresponding detection kit which comprises a primer amplifying the rs3820189 site, and a primer amplifying a region of Mfn2 gene 5' guide sequence, comprising the rs3820189 site. The method is used for detecting the gene type of the rs3820189 site, and is simple and practical, quick and high-efficiency, and lower in cost, so that a simple new way for diagnosing and treating the high blood pressure is provided.

The invention discloses a method for distinguishing the gene types of fast and slow featherings of chicks and a method for distinguishing the sexes of the chicks. According to the method for distinguishing the gene types, DNA in the blood sample of a chick is subjected to PCR amplification; a used forward primer F is 5'-GCACATTCAAGTAAGCAGTAGTTT-3', and a reverse primer R is 5'-AAAAAAAAAAAAAATTGCACTTTAATAGTACCATCTATTC-3'; an amplified product is subjected to digestion through a TaqI incision enzyme; the lengths of the segments after digestion are detected; the feathering rate gene types of the chick is judged on the basis of the lengths. According to the method for distinguishing the sexes of the chicks, a matching system is established based on the feathering rate gene types obtained through the method for distinguishing the gene types, and the sex of the chick is distinguished according to the characters of the fast and slow featherings. According to the invention, various gene types of fast and slow featherings of the chicks can be effectively distinguished, and the time for establishing the matching system of distinguishing the sexes of the chicks through the feathering rates is greatly shortened.

The invention relates to a kit for detecting a hepatitis c virus genotype, particularly relates to that the nucleic acid reverse dot blot hybridization technology is used to prepare a kit for hepatitis c virus genotype detection. The invention is used for rapidly and accurately distinguishing the hepatitis c virus genotype in a clinical blood sample.

The invention discloses a primer and probe composition for detecting polymorphism of human CYP2C9 and VKORC1 genes, a kit and application. The primer and probe composition comprises three pairs of specific primers for amplifying CYP2C9*2, CYP2C9*3 and VKORC1 sites, and three specific fluorescent probes. The primers and the probes, disclosed by the invention, have high sensitivity, strong specificity and strong anti-interference capability; a manner combining an asymmetric PCR (Polymerase Chain Reaction) amplification and fluorescent probe melting curve analysis technologies is used for detecting the gene polymorphism; different gene types can be effectively distinguished according to the quantity and Tm value of a melting peak; a result is convenient, clear and objective to judge and read.Single-tube sampling can be used for simultaneously detecting 6 mutation types of 3 gene sites; the primer and probe composition is simple and convenient to operate and the detection efficiency is improved; a large batch of samples can be detected and clinical operation is facilitated.

The invention belongs to the technical field of aquatic breeding, and particularly relates to a molecular marker related to growth of litopenaeus vannamei and application thereof. The molecular marker is located at the 109bp position of SEQM1286, and is a T / C type mutant; the individual growth speed of a CC gene in the marker is higher than the growth speed of a TT gene, that is to say, the CC gene type is a growth-advantage gene type. The molecular marker can be used as the molecular marker of prawn breeding to assist the genetic breeding of prawns.

The invention discloses an MTHFR, MTRR and RFC1 gene polymorphism detection primer combination and kit and application of the MTHFR, MTRR and RFC1 gene polymorphism detection kit. The detection kit comprises ultrapure water, an X solution, a 10*PCR buffering solution, a PCR primer, a 25 mM magnesium chloride solution, DNA polymerase and a positive control product, wherein the PCR primer comprises a forward and reverse amplification primer, a DNA internal reference forward and reverse amplification primer and a reaction internal reference forward and reverse amplification primer of different gene types, the three primers are located at four SNP sites on a gene related to folic acid metabolism, and the gene sequence of the PCR primer is shown in SEQ ID NO.1-NO.20. The detection kit has the advantages of being high in specificity, accuracy, flux and reliability, low in cost and free of a false-negative result.