160 results about "Ebola virus" patented technology

The present invention provides a therapeutic method for treating biological diseases that includes the administration of an effective amount of a suitable antibiotic agent, antifungal agent or antiviral agent in conjunction with an A_2A adenosine receptor agonist. If no anti-pathogenic agent is known the A_2A agonist can be used alone to reduce inflammation, as may occur during infection with antibiotic resistant bacteria, or certain viruses such as those that cause SARS or Ebola. Optionally, the method includes administration of a type IV PDE inhibitor.

The present invention provides compositions and devices comprising nanostructure networks, and related methods. The compositions may exhibit enhanced interaction between nanostructures, providing improved device performance (e.g., improved conductivity). In some embodiments, the devices are capable of interacting with various species to produce an observable signal from the device. In some cases, the compositions and devices may be useful in the determination of analytes, including—biological analytes (e.g., DNA, ebola virus, other infective agents, etc.), small, organic analytes, and the like. The embodiments described herein may exhibit high sensitivity and specificity to analytes and may be capable of analyte detection at femtomolar concentrations (e.g., 10 fM).

The invention discloses an antigen fragment and truncation based on ebola virus envelope protein as well as an application. The protein or the truncation of the protein is disclosed by the invention; the protein is shown in SEQ ID No. 3 or protein which is obtained by replacing and/or deleting and/or adding one or more amino acid residues in an amino acid sequence shown in the SEQ ID No. 3 and has the identical function as the protein shown in SEQ ID No. 3. The antigen fragment and the truncation thereof disclosed by the invention can induce relatively strong humoral immune response, has good immunogenicity and can be applied to development of ebola virus vaccines and preparation of neutralizing antibodies.

The invention relates to a hybridoma cell strain ZJEB8-01, an Ebola-virus GP albumen resistant monoclonal antibody, and preparation and application of the Ebola-virus GP albumen resistant monoclonal antibody. The hybridoma cell strain ZJEB8-01 can be used for secreting the Ebola-virus GP albumen resistant monoclonal antibody. Binding of the monoclonal antibody and the 412th-431st amino acid sequence antigen peptides of Ebola-virus GP albumen has high specificity and sensitiveness, and the monoclonal antibody can be applied to preparation of Ebola-virus GP albumen detection reagents. Sequencing of the monoclonal antibody is completed, and the material basis is laid for humanization transformation of monoclonal antibody sequences, research and development of the neutralizing antibody for neutralizing Ebola viruses and development of the antibody into clinical Ebola virus treatment antibody in later period.

The invention features compositions, methods, and kits useful for the treatment of filovirus-mediated diseases, e.g., hemorrhagic fever caused by Ebola virus, in an animal.

The present invention provides novel nanoparticle presented vaccine compositions that are stabilized with a locking domain. Various immunogens can be employed in the preparation of the vaccine compositions, including viral immunogens such as HIV-1 and Ebola viral immunogens, and non-viral immunogens such as immunogens derived from bacteria, parasites and mammalian species. The invention also provides methods of using such vaccine compositions in various therapeutic applications, e.g., for preventing or treating viral infections.

The invention discloses a method, a reagent, a primer and a probe for quickly detecting Ebola viruses under constant-temperature and isothermal conditions. The method, the reagent, the primer and the probe have the advantages that the Ebola viruses can be quickly, sensitively and accurately detected by the aid of the method in real time under the constant-temperature and isothermal conditions, nucleic acid targets can be quickly detected in instruments with fluorescence detection functions under the combined actions of the specific primer, the specific fluorescence probe, six engineering enzymes and other chemical constituents, closed-tube detection can be guaranteed by strict experiment operation steps, and aerosol pollution can be effectively prevented; the method, the reagent, the primer and the probe are applicable to detecting the Ebola viruses by the aid of diversified fluorescence detection devices.

The invention discloses L-nucleoside compounds having the structure characteristic represented by the formula (I) or pharmaceutically acceptable salts thereof, and belongs to the technical field of pharmaceutical chemistry. The compounds can inhibit the activity of RNA viral polymerase, so the compounds can be used as potential drugs for prevention and treatment of infection of RNA viruses such as HCV, influenza virus, HRV (rhinovirus), RSV, Ebola virus, dengue virus, intestinal virus and the like.

The invention discloses a crRNA target point and CRISPR-Cas13a system for detecting an Ebola virus. The CRISPR-Cas13a system comprises Cas13a protein and crRNA, or a composite formed from the Cas13a protein and the crRNA, and the crRNA comprises an anchoring sequence being combined with the Cas13a protein and a guiding sequence targeted to the sequence of the target point of the Ebola virus; and the sequence of the target point of the Ebola virus is located at the 1001-1028th of the genome of the Ebola virus. The crRNA can realize high-sensitivity and high-specifity detection on the nucleic acid of the Ebola virus by activating Cas13a, and the sensitivity achieves 1 copy/test (1copy/test).

The invention discloses a fluorescent quantitative PCR (polymerase chain reaction) method, primer and kit for detecting the EBOV (Ebola virus). The general method can be used for detecting that the sample to be detected is positive as long as the sample contains one or more of the five types of subtype EBOVs which are Z, S, B, C and R at the same time. The method overcomes the defects of the conventional PCR method for detecting by adopting the advantages of high-efficiency nucleic acid amplification of the PCR technology and the sensitivity of the fluorescence-dye SYBR Green I and the computer-aided fluorescent technology for detecting and improves the detection sensitivity, specificity and operation convenience greatly. In addition, the positive control adopted by the method is a section of RNA molecules transcribed in vitro of a NP gene, and the method is safer than the method for detecting by taking the inactivated virus solution as the positive control. The RNA molecules transcribed in vitro can be prepared in quantity, and the sources of the positive control are stable and reliable.

The invention relates to a hybridoma cell strain ZJED0-02, an anti-Ebola virus GP (glycoprotein) monoclonal antibody and their preparation and application. The hybridoma cell strain ZJED0-02 is applicable to secreting the anti-Ebola virus GP monoclonal antibody. The monoclonal antibody is highly specific and sensitive to 411th to 490th amino acid sequence antigen peptides of Ebola virus GP and is applicable to the preparation of Ebola virus GP detection reagents. The invention also finishes sequencing of the monoclonal antibody, a neutralizing antibody capable of neutralizing Ebola virus is developed for humanization of monoclonal antibody sequences, and material basis is laid of late development of the neutralizing antibody into antibodies for clinically treating Ebola virus diseases.

The present invention provides a therapeutic method for treating biological diseases that includes the administration of an effective amount of a suitable antibiotic agent, antifungal agent or antiviral agent in conjunction with an A_2A adenosine receptor agonist. If no anti-pathogenic agent is known the A_2A agonist can be used alone to reduce inflammation, as may occur during infection with antibiotic resistant bacteria, or certain viruses such as those that cause SARS or Ebola. Optionally, the method includes administration of a type IV PDE inhibitor.

The present invention provides compositions comprising therapeutic nucleic acids (e.g., interfering RNA such as siRNA) that target Ebola virus (EBOV) gene expression and methods of using such compositions to silence EBOV gene expression. More particularly, the invention provides unmodified and chemically modified interfering RNA which silence EBOV gene expression and methods of use thereof, e.g., for preventing or treating EBOV infections caused by one or more EBOV species such as Zaire EBOV. The invention also provides serum-stable nucleic acid-lipid particles comprising one or more interfering RNA molecules, a cationic lipid, and a non-cationic lipid, which can further comprise a conjugated lipid that inhibits aggregation of particles. Methods of silencing EBOV gene expression by administering one or more interfering RNA molecules to a mammalian subject are also provided.

Azole nucleosides represented by the formulae (I) and (II); wherein A=C or N B═C or N X═H; C₁-C₆ alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, aryl, heterocyclo, halogen such as F, Cl, Br and I; OH, NH₂, NH—(C₁-C₆ alkyl, cycloalkyl, aryl or heterocyclo); Z═H; C₁-C₆ alkyl, cycloalkyl, alkynyl, aryl, heterocyclo, halogen such as F, Cl, Br, I; OH NH₂, NH—(C₁-C₆ alkyl, cycloalkyl, aryl or heterocyclo; E=(CH₂)HONHR; n is an interger from 0-6 and more typically 0-3; R¹⁼ aryl or heterocyclo; each of W, Y, R is individually selected from the group consisting of H; C₁-C₆ alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, aryl, heterocyclo, halogen such as F, Cl, Br, and I; O, OH, Oalkyl, Oaryl, NH₂, NH(C₁-C₆ alkyl, cycloalkyl, aryl or heterocyclo); provided that at least one of W, Y, and R is other than H and wherein both W and Y together can be ═O; and each D individually is OH, Oalkyl, Oaryl, FL and H; pharmaceutically acceptable salts thereof, prodrugs thereof and mixtures thereof are provided. Compounds of this disclosure are useful as inhibitors of viral RNA and DNA polymerases such as, but not limited to, Influenza, hantaan Virus, Crimean Congo hemorrhagic fever virus, hepatitis B, hepatitis C, Polio, Coxsackie A and B, Rhino, Echo, orthopoxvirus (small pox), HIV, Ebola, and West Nile virus polymerases; and especially orthopoxvirus, HIV, and hepatitis B.

The invention discloses Marburg and Ebola dual-virus fluorescent quantitative PCR (Polymerase Chain Reaction) detection method and system, wherein the detection system comprises primers, probes, a Premix EX Taq reaction solution and sterilizing Tris water. As two pairs of primers and probes have very good specificity, the detection system has high sensitivity and is suitable for simultaneously detecting Marburg and Ebola viruses without having cross reaction with other kinds of hemorrhagic fever arbovirus, such as yellow fever, dengue and rift valley fever.

The invention provides a RPA (recombinase polymerase amplification) kit used for detecting Ebola virus, special-purpose primers and probes of the RPA kit, and applications of the RPA kit in detection of Ebola virus. The RPA kit, the special-purpose primers, and the probes are designed based on Ebola virus NP gene conserved sequence, and the Ebola virus NP gene conserved sequence possesses an oligonucleotide sequence represented by SEQ ID NO.1. It is shown by research results that the RPA kit can be used for rapid detecting of Ebola virus in samples with specificity and sensitivity.

Nucleic acid molecules and compositions comprising one or more nucleic acid sequences that encode a consensus filovirus immunogen including a consensus Marburgvirus filovirus glycoprotein MARV GP immunogen, a consensus Ebolavirus Sudan filovirus glycoprotein SEBOV GP immunogen and a consensus Ebolavirus Zaire glycoprotein ZEBOV GP immunogen are disclosed. The coding sequences optionally include operable linked coding sequence that encode a signal peptide. Immunomodulatory methods and methods of inducing an immune response against filovirus, particularly Marburgvirus, Ebolavirus Sudan and Ebolavirus Zaire are disclosed. Method of preventing filovirus infection, particularly infection by Marburgvirus, Ebolavirus Sudan and Ebolavirus Zaire and methods of treating individuals infected with filovirus infection, particularly infection by Marburgvirus, Ebolavirus Sudan and Ebolavirus Zaire are disclosed. Consensus filovirus proteins are disclosed.

The instant invention is drawn to methods useful for the treatment or the prevention of a viral infection. The methods include administering at least one compound that is an inhibitor of cathepsin L to an individual. The methods are particularly useful in individuals infected with, or at risk of infection with, SARS virus or Ebola virus. The invention also includes methods of identifying potential therapeutics for use in the methods of treatment or prevention of a viral infection.

The invention discloses a fulminating-infectious-disease pathogen detecting primer pair and a kit. The primer pair comprises at least a pair of RT-LAMP primers of Ebola viruses, Lassa fever viruses, Marburg viruses, rift valley fever viruses, yellow fever viruses and Chikungunya fever viruses. By means of the primer system, the amplification reaction background is reduced, and sensitivity and specificity are quite good. The kit formed by the primer pair further comprises detecting liquid and a micro-fluidic chip; as an independent RT-PCR secondary amplification step of a detecting liquid system is omitted, detecting time is shortened; as the denaturation process and the renaturation process of nucleic acid do not exist, the polluted chance of RNA enzymes and the polluted chance of amplification nucleic acid are reduced, and the sensibility and the safety of detection are improved. By means of the constant-temperature sealed environment provided by a micro-fluidic chip system, rapid and constant-temperature amplification and automation result distinguishing of a nucleic acid extracting template are finally achieved, the requirement for test hardware is reduced, the use level of a reaction reagent is reduced, detection cost is reduced, and the result can be directly determined through color changes.

Disclosed are methods of treating, inhibiting, or preventing a viral infection in a mammal in need thereof by administering a therapeutically or prophylactically effective amount of an inhibitor of FAP, an inhibitor of DPPIV, an inhibitor of DPP8, or an inhibitor of DPP9. The inhibitor may act as both an inhibitor of DPPIV and an inhibitor of DPP8/9. The viral infection includes, but is not limited to, hepatitis B virus, hepatitis C virus, human immunodeficiency virus, Polio virus, Coxsackie A virus, Coxsackie B virus, Rhino virus, respiratory syncytial virus, dengue virus, equine infectious anemia virus, Echo virus, small pox virus, Ebola virus, and West Nile virus.

The invention belongs to the field of immunology and molecular biology, and relates to an anti-Ebola virus monoclonal antibody, a preparation method and uses thereof, specifically to an anti-Ebola virus monoclonal antibody or an antigen-binding part thereof, wherein the amino acid sequences of the light chain variable region and the heavy chain variable region comprise one group selected from thefollowing groups (1)-(3): the group (1): the amino acid sequence of the light chain variable region is represented by SEQ ID NO:4, and the amino acid sequence of the heavy chain variable region is represented by SEQ ID NO:6; the group (2): the amino acid sequence of the light chain variable region is represented by SEQ ID NO:10, and the amino acid sequence of the heavy chain variable region is represented by SEQ ID NO:12; and the group (3): the amino acid sequence of the light chain variable region is represented by SEQ ID NO:16, and the amino acid sequence of the heavy chain variable region is represented by SEQ ID NO:18. According to the present invention, the anti-Ebola virus monoclonal antibody has advantages of enhanced ADCC activity, good antigen-binding activity and good virus-neutralizing activity.

The invention provides a novel infection model based on EBOV (Ebola Virus) virus-like particles (VLP) and taking firefly luciferase (Fluc) as a reporter protein. The model enters cells with the help of an EBOV envelope protein (GP); the Fluc can be introduced into the cells after the model enters the cells, so that cell infection can be quantified according to signal intensity of the Fluc in the cells; mouse infection can be quantified through biological imaging according to signal intensity of bioluminescence in a mouse body. Therefore, the inventor can utilize the model to carry out in-vitro and in-vivo pre-screening experiments on a neutralizing antibody and an entered inhibitor of the GP outside a BSL-4 (Biosafety Level-4) laboratory.