68 results about "Hemorrhagic fever virus" patented technology

Compounds, methods and pharmaceutical compositions for treating viral infections, by administering certain novel compounds in therapeutically effective amounts are disclosed. Methods for preparing the compounds and methods of using the compounds and pharmaceutical compositions thereof are also disclosed. In particular, the treatment and prophylaxis of viral infections such as caused by hemorrhagic fever viruses is disclosed, i.e., including but not limited to, Arenaviridae (Junin, Machupo, Guanarito, Sabia, Lassa, Tacaribe, Pichinde, and LCMV), Filoviridae (Ebola and Marburg viruses), Flaviviridae (yellow fever, Omsk hemorrhagic fever and Kyasanur Forest disease viruses), and Bunyaviridae (Rift Valley fever and Crimean-Congo hemorrhagic fever).

An application of indole-2.3-bione in preparing the antiviral medicine for HIV, influenza virus, hemorrhagic virus and herpes simplex virus and the immunopotentiator is disclosed.

The invention discloses a fluorescence quantitative polymerase chain reaction (PCR) new method for detecting multiple viruses of yellow fever, dengue fever and epidemicencephalitis B and multiple virus detection PCR system consisting of primers, probes, a Premix Ex Taq reaction solution and a sterilized Tris buffer. With good singularity and high sensitivity, three pairs of primers and probes are very suitable for simultaneously detecting viruses of yellow fever, dengue fever and epidemicencephalitis B. And there is no cross reaction between the primers and probes and several other entomophily hemorrhagic fever viruses, such as Marburg virus and Rift Valley fever virus.

The invention discloses an RPA technology-based marburg virus detection kit and an application thereof. An experiment proves that a target gene can be effectively amplified by an RPA primer and a probe of a marburg virus, the specificity reaches 100% and sensitivity is 1*10<2>copies / reaction; and the virus can reach the level equivalent to the sensitivity of fluorescent quantitative PCR, and has no cross reaction with a zaire ebola pseudovirus, a dengue virus, a hemorrhagic fever virus with renal syndrome and a Xinjiang hemorrhagic fever virus nucleic acid. The RPA isothermal amplification system is fast in reaction and wide in temperature range, effective amplification of a target gene can be achieved under the condition of 37-42 DEG C, the method can be used for fast field detection of an infectious nucleic acid of the marburg virus, and an available fast detection method is provided for field screening of a pathogen; and meanwhile, the marburg virus detection kit also has the important significance in prevention of infection transmission of the marburg virus in China and inspection and quarantine in affected areas and entry and exit ports.

Azole nucleosides represented by the formulae (I) and (II); wherein A=C or N B═C or N X═H; C1-C6 alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, aryl, heterocyclo, halogen such as F, Cl, Br and I; OH, NH2, NH—(C1-C6 alkyl, cycloalkyl, aryl or heterocyclo); Z═H; C1-C6 alkyl, cycloalkyl, alkynyl, aryl, heterocyclo, halogen such as F, Cl, Br, I; OH NH2, NH—(C1-C6 alkyl, cycloalkyl, aryl or heterocyclo; E=(CH2)HONHR; n is an interger from 0-6 and more typically 0-3; R1= aryl or heterocyclo; each of W, Y, R is individually selected from the group consisting of H; C1-C6 alkyl, cycloalkyl, alkenyl, cycloalkenyl, alkynyl, aryl, heterocyclo, halogen such as F, Cl, Br, and I; O, OH, Oalkyl, Oaryl, NH2, NH(C1-C6 alkyl, cycloalkyl, aryl or heterocyclo); provided that at least one of W, Y, and R is other than H and wherein both W and Y together can be ═O; and each D individually is OH, Oalkyl, Oaryl, FL and H; pharmaceutically acceptable salts thereof, prodrugs thereof and mixtures thereof are provided. Compounds of this disclosure are useful as inhibitors of viral RNA and DNA polymerases such as, but not limited to, Influenza, hantaan Virus, Crimean Congo hemorrhagic fever virus, hepatitis B, hepatitis C, Polio, Coxsackie A and B, Rhino, Echo, orthopoxvirus (small pox), HIV, Ebola, and West Nile virus polymerases; and especially orthopoxvirus, HIV, and hepatitis B.

The invention discloses Marburg and Ebola dual-virus fluorescent quantitative PCR (Polymerase Chain Reaction) detection method and system, wherein the detection system comprises primers, probes, a Premix EX Taq reaction solution and sterilizing Tris water. As two pairs of primers and probes have very good specificity, the detection system has high sensitivity and is suitable for simultaneously detecting Marburg and Ebola viruses without having cross reaction with other kinds of hemorrhagic fever arbovirus, such as yellow fever, dengue and rift valley fever.

The invention relates to the field of genetic engineering and particularly relates to a preparation method of a rabbit hemorrhagic fever virus empty capsid antigen. The method provided by the invention comprises the following steps: 1) constructing a rhabdovirus transfer carrier containing a rabbit hemorrhagic fever virus capsid protein VP60 gene or an optimized gene, wherein codon optimization is performed according to the codon frequency of bombyx mori; 2) performing cotransfection on the constructed transfer expression carrier and DNA (deoxyribonucleic acid) of rhabdovirus so as to carry out homologous recombination or transposition to further obtain the recombinant rhabdovirus; 3) infecting the recombinant rhabdovirus with the host cells of an insect; and 4) culturing the infected host of the insect to express the corresponding rabbit hemorrhagic fever virus empty capsid antigen, and harvesting and purifying the expressed antigen. By adopting the method provided by the invention, the production cost of the rabbit hemorrhagic fever virus empty capsid antigen can be greatly reduced, and the method has a plurality of advantages of safety, high efficiency, low energy consumption, low cost and the like.

The present invention generally relates to compositions comprising benzonapthyridine small molecule immune potentiators (SMIPs) that are capable of stimulating or modulating an immune response in a subject that has had pre- or post-exposure to a pathogen such as hemorrhagic fever virus. Also provided are methods of preparing and using the SMIP compositions of the invention.

The invention relates to the technical field of biological detection, in particular to a detection method of hemorrhagic fever with renal syndrome IgM (immunoglobulin M) antibodies and a reagent kit. The detection method comprises the steps that hemorrhagic fever with renal syndrome antigens are fixed on a membrane; to-be-detected serum is added; the hemorrhagic fever with renal syndrome IgM antibodies in the to-be-detected serum are combined with the antigens fixed on the membrane; gold labeled operating fluid containing mouse anti-human IgM monoclonal antibodies is added; the mouse anti-human IgM monoclonal antibodies are combinec with the hemorrhagic fever with renal syndrome IgM antibodies in the to-be-detected serum; a red color is shown; scrubbing liquid is added finally; and the redundant gold labeled operating fluid and other impurities are washed out. The reagent kit has the characteristics of longer quality guarantee period, less cross reaction, better color rendering performance and the like.

This invention provides one test bar, which comprises the following steps: a, covering antibody IgM linkage single clone antibody and anti-flu hemorrhagic fever virus reset antigen multiple clone antibody two NC fiber films; b, comprising glue gold label flu hemorrhagic fever virus glass fiber film; applying film analysis and glue gold label technique to test sample flu virus antibody.

The invention relates to the technical filed of antigen genes, in particular to a Xinjiang hemorrhagic fever virus nucleoprotein antigen gene (XHFNP235) as well as recombinant protein and application thereof. The gene has a nucleotide sequence as sequence 1. In the invention, the Xinjiang hemorrhagic fever virus nucleoprotein antigen gene (XHFNP235) and the recombinant protein thereof are obtained from Xinjiang hemorrhagic fever virus. Through PCR amplification, cloning, transformation, expression plasmid construction, induction expression and immunoassay detection, the Xinjiang hemorrhagic fever epitope is positioned at the 235th to 305th amino acid region of NP protein, and the region is a highly conservative amino acid region of the NP protein, which further shows that the XHFNP235 recombinant protein can be a candidate diagnosis antigen for Xinjiang hemorrhagic fever, and provides a new way for diagnosing and applying Xinjiang hemorrhagic fever.

The invention involves nephrotic syndrome hemorrhagic fever Vero cells purified bivalent vaccine and its industrialized preparation methods. The nephrotic syndrome hemorrhagic fever Vero cells bivalent purified vaccine in the inventioncontains effective doses of nephrotic syndrome hemorrhagic fever deactive virus type I, type II and vaccine adjuvants, the type I and type II virus are from PS-6 (C-3) strains, CCTCC-V200503 and L99(C-2) strains, CCTCC-V200504 respectively. The preparation method of the invention includes: 1. Preparating the vaccine stock solution nephrotic syndrome hemorrhagic fever virus PS-6(C-3), CCTCC-V200503 and L99(C-2),CCTCC-V200504, inactivating virus with formaldehyde; 2. combining the deactive vaccine stock solution of PS-6(C-3), CCTCC-V200503 and L99(C-2),CCTCC-V200504 and purifying, the process contains centrifugal separation, hyperfiltration, concentration, zinc acetate deposition and column chromatography; 3. adding adjuvant into the purified vaccine stock solution and getting the purified bivalent vaccine. The nephrotic syndrome hemorrhagic fever Vero cells purified bivalent vaccine in the invention is suitable for the prevention of nephrotic syndrome hemorrhagic fever mainly based on all type I and type II.

The compositions and methods are described for generating an immune response to a hemorrhagic fever virus such as ebolavirus, Marburgvirus, or arenavirus. The compositions and methods described herein relate to a modified vaccinia Ankara (MVA) vector encoding one or more viral antigens for generating a protective immune response to a member of genus Ebolavirus (such as a member of species Zaire ebolavirus), a member of genus Marburgvirus (such as a member of species Marburg marburgvirus), or a member of genus Arenavirus (such as a member of species Lassa virus) in the subject to which the vector is administered. The compositions and methods of the present invention are useful both prophylactically and therapeutically and may be used to prevent and / or treat an infection caused by ebolavirus, Marburgvirus, or arenavirus.

The genetically modified hemorrhagic fever virus of this invention possesses a viral ovarian tumor protease with decreased ability to remove ubiquitin (Ub) and ISG15 tags that the human organism uses to label proteins for removal. Unlike complete knockout strains, the modified virus retains enough activity for replication in a human cell line. This creates an immunogenic and non-pathogenic virus that can be used as an effective live vaccine agent.

The invention discloses a nano-gold biosensor for simultaneously detecting four hemorrhagic fever viruses and a detection method thereof. The biosensor comprises a substrate, a sample pad, an absorption pad, a nitrocellulose film and a bonding pad, wherein the sample pad and the absorption pad are fixed at two ends of the substrate, and the nitrocellulose film is fixed at the middle part of the substrate; a 2-3mm overlap is formed between the absorption pad and the nitrocellulose film, a 2-3mm overlap is formed between the nitrocellulose film and the binding pad, and a 2-3mm overlap is formedbetween the binding pad and the sample pad; and gold nanoparticle DNA probes are arranged on the binding pad. The biosensor has the advantages of high sensitivity, high specificity, short detection time, capability of completing detection within 60 minutes and low detection cost.

The invention discloses a preparation method of a Crimean-Congo hemorrhagic fever virus nucleic acid molecule characteristic standard sample. The Crimean-Congo hemorrhagic fever virus nucleic acid molecule characteristic standard sample is prepared through sequence synthesis, vector cloning, target fragment and vector connection, plasmid transformation, recombinant plasmid extraction, freeze-drying and preservation, includes virus characteristic sequence information, and is suitable for analyzing the virus and the content value of the virus. The standard sample provided by the invention has the advantages of good uniformity, high stability, long-time storage and good purity. The preparation method has a simple process, can provide the standard sample for detection research and medical research of Crimean-Congo hemorrhagic fever virus to realize the comparison of different laboratory results in order to ensure the laboratory quality control, and also provides the standard sample for the rapid and accurate detection of Crimean-Congo hemorrhagic fever virus by inspection and quarantine institutions, and import and export trade enterprises.

The invention provides a Xinjiang hemorrhagic fever virus immunochromatography rapid detection test paper comprising a supporting and fixing adhering lining layer on which a sample absorbing layer, a gold-marking binding pad, a fibrin film layer and a water absorbing layer are sequentially adhered to the supporting and fixing adhering lining layer, wherein the fibrin film layer takes a monoclonal antibody which is printed with Xinjiang hemorrhagic fever virus nucleocapsid protein as a detection blot zone. The fibrin film layer of the Xinjiang hemorrhagic fever virus immunochromatography rapid detection test paper is printed with sheep-anti-mouse IgG antibody solution as a contrapositive blot zone. A monoclonal antibody of the Xinjiang hemorrhagic fever virus nucleocapsid protein which is marked by colloidal gold is absorbed on the gold-marking binding pad of the test paper. The Xinjiang hemorrhagic fever virus immunochromatography rapid detection test paper can effectively and rapidly detect the Xinjiang hemorrhagic fever virus.

The invention provides a reagent kit capable of simultaneously detecting virulent viruses and bacteria and a detecting method. The reagent kit is mainly in accordance with 4 pathogenic microorganismsof ebola viruses, Sinkiang hemorrhagic fever viruses, Brucella and anthrax bacillus. The reagent kit comprises a specific primer probe combination for detecting the ebola viruses, the Sinkiang hemorrhagic fever viruses, the Brucella and the anthrax bacillus, a micro-fluidic solid-phase PCR chip on which a specificity probe is fixed, and an RNase-resistant high-stability positive quality control product consisting of virus-like particles containing viral nucleic acid and thalli containing plasmid carrying the specificity nucleic acid. According to the reagent kit, simultaneous detection of theebola viruses, the Sinkiang hemorrhagic fever viruses, the Brucella and the anthrax bacillus can be realized, and the reagent kit has the advantages of being high in detection flux, high in sensitivity, high in specificity, good in repeatability, short in detection time, low in detection cost, low in operation technique requirements, not liable to pollute and the like, and has great application prospects in the field of quick simultaneous detection of various pathogenic microorganisms.

The invention provides a test card for rapid detection of an epidemic hemorrhagic fever virus antibody. The test card comprises a bottom plate, a sample pad, a nitrocellulose membrane, a water absorption pad and a fluorescent microsphere pad, wherein the fluorescent microsphere pad adsorbs fluorescent microspheres marked by epidemic hemorrhagic fever virus antigens and fluorescent microspheres marked by quality control proteins; and an anti-human IgG antibody, an anti-human IgM antibody and an anti-quality control protein antibody are sequentially adsorbed on the nitrocellulose membrane. In addition, the invention provides a preparation method of the test card, an application of the test card to the rapid detection of the epidemic hemorrhagic fever virus human antibody, and the like.

The invention relates to an inactivation biological product virus and removing bacterial endotoxin method. The biological product or middle product is adjusted its pH value to 12.0+ / -0.2 by NaOH solution, hatched for at least one hour at 25+ / -1 centigrade degree, then adjusted its pH value to need by HCl solution. The method has good inactivation effect for all fat enveloped virus or non. It has done a lot of test for many viruses. And all of them have good inactivation effect. It is qualified tested by TAL and rabbit detection pyrogen.

The compositions and methods are described for generating an immune response to a hemorrhagic fever virus such as ebolavirus, Marburgvirus, or arenavirus. The compositions and methods described herein relate to a modified vaccinia Ankara (MVA) vector encoding one or more viral antigens for generating a protective immune response to a member of genus Ebolavirus (such as a member of species Zaire ebolavirus), a member of genus Marburgvirus (such as a member of species Marburg marburgvirus), or a member of genus Arenavirus (such as a member of species Lassa virus) in the subject to which the vector is administered. The compositions and methods of the present invention are useful both prophylactically and therapeutically and may be used to prevent and / or treat an infection caused by ebolavirus, Marburgvirus, or arenavirus.

The invention relates to a fluorescence quantitative RT-PCR (reverse transcription-polymerase chain reaction) kit for rapidly detecting XHF (Xinjiang hemorrhagic fever) viruses. In the invention, a simple, rapid and sensitive RT-PCR detection kit is developed on the basis of designing a specific primer according to a specific conserved sequence of an XHF virus and successfully establishing a PCR detection method for XHF viruses. When XHF viruses are detected by using the kit and detection method provided by the invention, the detection process is convenient and rapid, a detection result can be obtained in 2-3 hours, and the detection sensitivity and the detection accuracy are high, therefore, the kit and detection method provided by the invention are especially suitable for the needs of entry-exit inspection and quarantine.

The genetically modified hemorrhagic fever virus of this invention possesses a viral ovarian tumor protease with decreased ability to remove ubiquitin (Ub) and ISG15 tags that the human organism uses to label proteins for removal. Unlike complete knockout strains, the modified virus retains enough activity for replication in a human cell line. This creates an immunogenic and non-pathogenic virus that can be used as an effective live vaccine agent.

The invention provides a kit for one-step nucleic acid quantitative RT-PCR detection on a Xinjiang hemorrhagic fever virus.The kit comprises RT-PCR reaction liquid, a RT-PCR enzyme mixture, XHFV nucleic acid quantitative primers and probes, an internal reference, negative control, critical positive control, strong positive control and calibrators (1-5).According to the kit, a one-step RT-PCR reaction can be directly performed on extracted XHFV RNA, fluorescent quantitative detection is performed on XHFV RNA in the samples, an internal reference gene sequence is taken as internal control, and contamination is prevented through a UNG enzyme; the kit is simple in one-step amplification method, short in procedure, easy and convenient to operate, capable of preventing contamination, high in specificity of a detection result and sensitivity, clear in result and high in credibility and can be applied to nucleic acid quantitative detection on the Xinjiang hemorrhagic fever virus in serum.

The invention discloses a new application of Lycojaponicumin C in preparation of a medicine for treating hemorrhagic fever with renal syndrome. Experimental researches of Lycojaponicumin C in-vitro and in-vivo inhibition on virus of hemorrhagic fever with renal syndrome indicate that Lycojaponicumin C has low toxicity to cells, can directly kill virus of hemorrhagic fever with renal syndrome, can remarkably inhibit multiplication of the virus, and can obviously protect shrewmouse infected by the virus so as to delay the disease time, prolong the course of disease and reduce the death rate. Therefore, the Lycojaponicumin C is an effective and safe medicine for treating hemorrhagic fever with renal syndrome.