94 results about "Immunopotentiator" patented technology

The invention belongs to the technical field of biological medicines, and particularly relates to application of bleomycin or bleomycin analogues in preparation of an immunopotentiator. The invention also provides a pharmaceutical composition and application of the pharmaceutical composition in preparation of drugs for treating and / or preventing cancers. The pharmaceutical composition comprises bleomycin, bleomycin analogues or a combination thereof and an anti-tumor immunotherapeutic agent.

The invention relates to the technical field of aquaculture fish vaccines, in particular to a fusion gene of grass carp NPM1a and II type reovirus VP38, recombinant protein, a composition and application. The nucleotide sequence of the fusion gene is as shown in SEQ ID NO: 1. The fusion gene is obtained by connecting grass carp NPM1a and II type reovirus VP38 through a linker sequence, a BamH restriction enzyme cutting site is added to the 5'end of the fusion sequence, and an XhoI restriction enzyme cutting site is added to the 3 'end of the fusion sequence; the sequence of the linker is as shown in SEQ ID NO: 2. The subunit vaccine of the fusion protein of the grass carp NPM1a and the II type reovirus VP38 is prepared, and indole-3-lactic acid is used as an immunopotentiator in a combined manner, so that the technical bottlenecks of low protection rate, poor intestinal mucosal barrier repairability and the like of the existing vaccine are overcome.

The invention belongs to the technical field of animal immunopotentiators, and particularly relates to an oil-in-water type veterinary adjuvant and a preparation method thereof. The oil-in-water type veterinary adjuvant comprises white oil, span 80, Tween 80, PEG400, astragalus polysaccharide and a phosphate buffer solution, the astragalus polysaccharide cooperates with a surfactant to activate a TLR4 / NF-kappa B pathway, Th1 / Th2 / Th17 three-way immune response is achieved, the serum IgG and mucous membrane sIgA antibody level is remarkably improved, and the problems that a traditional O / W adjuvant is high in layering rate (smaller than 5%), and the stability of the traditional O / W adjuvant is poor are solved through compound optimization of the HLB value (HLB = 11.5-12.0) of the HLB value of span 80 and Tween 80. And storage at 4 DEG C for 90 days) and poor freeze-thaw stability are solved.

The invention discloses an immunogenic composition containing an adjuvant as well as a preparation method and application of the immunogenic composition. The immunogenic composition comprises self-assembled gE virus-like nanoparticles, an immunopotentiator, namely, saponin QS-21, and a neutral liposome, the self-assembled gE virus-like nanoparticles are formed by polymerizing and assembling monomers; the monomers include VZV gE, a linker peptide (SGS), and a VZV gI polypeptide containing a Th epitope. The immunogenic composition can be applied to varicella-zoster virus vaccines, and solves the technical problems of weak gE immunogenicity and serious vaccine side reaction in vaccines prepared in the prior art. The immunogenicity of the vaccine is equivalent to that of the Xinanlii, and the use of an immunopotentiator in the vaccine can be reduced, so that the clinical side reaction of the vaccine is lower; in addition, the vaccine can induce a gI specific antibody and gI specific CMI reaction, and the effectiveness of the vaccine can be further improved. In short, the immunogenic composition has good clinical application potential.

The invention relates to a fungus-diatom synergistic immunopotentiator for stichopus japonicus, and belongs to the technical field of microbial engineering and echinoderm immunology. The immunopotentiator is a symbiont microsphere taking yarrowia lipolytica and navicula seminuda as main components; the method comprises the following steps: respectively activating yarrowia lipolytica and navicula seminuda, carrying out expanding culture, resuspending, and adjusting the concentrations of a bacterial solution and algae cells; the preparation method comprises the following steps: adding a navicula seminuda concentrated solution and a yarrowia lipolytica suspension into a triangular flask containing a YPD liquid culture medium, adding selenium-containing fucoidan polysaccharide sulfate accounting for 0.4-1.2% of the total mass of microspheres, carrying out constant-temperature shake culture, filtering by using sterile gauze to obtain microspheres, and flushing by using sterile normal saline to obtain fungus-diatom symbiont microspheres; the yarrowia lipolytica, the navicula seminuda and the selenium-containing fucoidan polysaccharide sulfate in the prepared immunopotentiator are mutually beneficial and have a synergistic effect, and the non-specific immune function of the stichopus japonicus is comprehensively improved by improving the growth index, enhancing the activity of immune enzyme and digestive enzyme and improving the disease resistance.

The invention discloses an immunogenic composition containing a composite adjuvant as well as a preparation method and application of the immunogenic composition. The immunogenic composition comprises a gE-gI fusion protein, an immunopotentiator, namely, saponin QS-21, and a neutral liposome, the gE-gI fusion protein comprises a VZV gE, a connecting peptide and a VZV gI. The immunogenic composition provided by the invention only contains one immunopotentiator, can be applied to VZV vaccines, and solves the problems of relatively weak gE immunogenicity and relatively large clinical side reaction of the vaccines prepared in the prior art. The immunogenicity of the vaccine is equivalent to that of the Xinanlii, but the vaccine contains fewer immunopotentiators and has lower clinical side effects; in addition, the vaccine can also induce a gI specific antibody and gI specific CMI reaction, which is beneficial to further improvement of the effectiveness of the vaccine. In short, the immunogenic composition has good clinical application potential.

The present invention relates to a composition for enhancing immunity, containing, as an active ingredient, a polysaccharide enzyme-treated product derived from a mixed extract of Angelica gigas, Cnidium officinale, and Paeonia lactiflora, or a novel purified polysaccharide fraction thereof. The composition for enhancing immunity according to the present invention, comprising the polysaccharide enzyme-treated product of the mixed extract of Angelica gigas, Cnidium officinale, and Paeonia lactiflora or the novel purified polysaccharide fraction thereof, is composed of specific types and contents of sugars and significantly increases the activity of macrophages, splenocytes, and NK cells upon application thereto, thereby being applicable as an immunopotentiator or a composition for enhancing immunity.

The invention discloses a Chinese herbal medicine immunopotentiator for special fur economic animals as well as a preparation method and application of the Chinese herbal medicine immunopotentiator. Belongs to the technical field of traditional Chinese medicine preparations. The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 2-4 parts of echinacea purpurea, 2-4 parts of bighead atractylodes rhizome, 2-4 parts of Chinese yam, 1-2 parts of medlar and 1-2 parts of codonopsis pilosula. The raw material medicines are subjected to crushing, burdening, water boiling extraction, concentration and packaging to prepare the medicine. The Chinese herbal medicine immunopotentiator for the special fur economic animals has good effects of enhancing the disease resistance and disease prevention capability of racoon dog and improving the immunity.

The present invention belongs to the field of molecular biology technology, and in particular relates to a recombinant protein of the CgVg functional domain DUF1943 and VWD of the long oyster, as well as a preparation method and application thereof. The amino acid sequence of the recombinant protein of the CgVg functional domain DUF1943 of the long oyster is shown in SEQ ID NO.1. The amino acid sequence of the recombinant protein of the CgVg functional domain VWD of the long oyster is shown in SEQ ID NO.2. The recombinant proteins of the CgVg functional domain DUF1943 and VWD of the long oyster obtained by the present invention have a wide range of binding activity against microorganisms, among which the DUF1943 recombinant protein has the activity of agglutinating bacteria and fungi, and the VWD recombinant protein has a broad spectrum of activity in inhibiting bacterial growth, and can be applied to the research and development of antibacterial drugs, immunopotentiators and feed additives for marine organisms.

The invention relates to the technical field of biological medicine, in particular to an immune synergist, an enhanced NK cell preparation, a preparation method and application. The immune synergist is hirudin, and the concentration of the hirudin is 10-40 [mu] g / ml. The enhanced NK cell preparation is prepared by the preparation method. According to the preparation method, the enhanced NK cell preparation is obtained after NK cells which are amplified in vitro for 14 days are pretreated by adopting the immune synergist. The application comprises an application of the immune synergist in up-regulation of expression of NK cell granzyme B and an application of the immune synergist in preparation of an NK cell preparation for inhibiting breast cancer cell activity. The expression level of granzyme B of the NK cells can be safely and economically improved, and the in-vitro killing activity of the NK cells to breast cancer cells is remarkably enhanced.

The invention relates to the technical field of immunization. The invention discloses parabacteroides dieldrii and parabacteroides goldsteinii, an application of the parabacteroides dieldrii and / or the parabacteroides goldsteinii in preparation of an immunopotentiator of a vaccine, and the immunopotentiator of the vaccine, and particularly discloses the parabacteroides dieldrii and the parabacteroides goldsteinii, the application of the parabacteroides dieldrii and / or the parabacteroides goldsteinii in preparation of the immunopotentiator of the vaccine. The parabacteroides dieldrii and / or the parabacteroides gold can be used as an immunopotentiator, can activate immunity, enhance antibody titer and shorten antibody generation reaction time, and can remarkably promote proliferation of B cells, plasma cells and memory B cell subgroups in a hair growth center.

The invention relates to the technical field of precipitation separation equipment, and discloses an immunopotentiator crude product precipitation separation device which comprises a precipitation tank used for precipitation separation of an immunopotentiator; the sealed tank body is used for being in sealed butt joint with the top of the precipitation tank body, and a feeding opening is formed in the top of the sealed tank body; the cleaning mechanism is used for covering the top of the precipitation tank body and cleaning the interior of the precipitation tank body; the lifting and rotating mechanism is used for switching butt joint of the sealing tank body or the cleaning mechanism and the settling tank body, the lifting and rotating mechanism is arranged between the sealing tank body and the cleaning mechanism, and the lifting and rotating mechanism is connected with the sealing tank body and the cleaning mechanism in a one-to-one mode through connecting rods. Maintenance and inspection are convenient, the daily maintenance process is greatly simplified, the working efficiency is improved, meanwhile, the mechanical damage risk possibly caused by frequent disassembly and assembly is reduced, and the service life of equipment is prolonged.

The invention discloses a gE fusion protein containing a gH Th epitope and a preparation method and application thereof, and relates to the technical field of biomedicine, the fusion protein comprises a varicella-zoster virus (VZV) glycoprotein E extracellular domain (gE) and a Th epitope-containing VZV glycoprotein H extracellular domain (gH) polypeptide. The gE fusion protein can be applied to VZV vaccines, and solves the technical problems of weak immunogenicity of gE antigens and serious side reaction of vaccines in vaccines prepared in the prior art. Compared with the Xinanlietaine, the vaccine taking the gE fusion protein as the antigen has the advantages that the variety and dosage of immunopotentiators are less, the immunogenicity is better, and the clinical side reaction is lower; besides, the gE fusion protein vaccine not only can induce a gE specific CMI reaction, but also can induce a gH specific CMI reaction, so that the effectiveness of the vaccine is further improved. In short, the gE fusion protein has good clinical application potential.

The application discloses a use of MHC-II expression in prediction of curative effect of gastric cancer immunotherapy and belongs to the field of bioengineering. Specifically, the application comprises the following steps: detecting MHC-II expression in a gastric cancer biopsy sample; baking, dewaxing and gradient alcohol treating the gastric cancer biopsy sample slice; carrying out antigen repairing and blocking treatment; then adding diluted MHC Class II antibody for incubation, and then adding immunopotentiator and enhanced enzyme-labeled antibody for incubation; finally, completing immunostaining through DAB color development, staining liquid staining and counter blue treatment, mounting and observing. The MHC-II expression signal is clear and distinguishable, the staining effect is good, and through negative and positive expression interpretation and pathological remission evaluation, it is confirmed that the MHC-II expression is significantly related to pathological remission of a gastric cancer patient and plays an important role in prediction of curative effect of gastric cancer immunotherapy.

The invention discloses an immunogenic composition containing gE virus-like nanoparticles as well as a preparation method and application of the immunogenic composition. The immunogenic composition comprises gE virus-like nanoparticles, an immunopotentiator, namely, saponin QS-21, and a neutral liposome, the gE virus-like nanoparticles comprise VZV gE, a linker peptide (SGS) and a VZV gI polypeptide containing a Th epitope. The immunogenic composition provided by the invention can be applied to VZV vaccines, and solves the technical problems of weak gE immunogenicity and serious vaccine side reaction in vaccines prepared in the prior art. The immunogenicity of the vaccine is better than that of Xinanliei, the use of an immunopotentiator is reduced, and the clinical side reaction is lower. In addition, the vaccine can also induce an obvious gI specific antibody, which is beneficial for further improving the effectiveness of the vaccine. In short, the immunogenic composition provided by the invention has good clinical application potential.

To provide an intestinal disorder controlling agent, an antiallergic agent, an immunopotentiator and an intestinal adhesion improver of lactic acid bacteria, safely ingestible, inexpensively available and excellent in intestinal adhesion improving action of lactic acid bacteria.SOLUTION: The agent of the present invention contains a flower extract. The flower is a flower of Lythraceae, Asteraceae, Fabaceae, Rosaceae or Iridaceae, and is particularly preferably at least one selected from the group consisting of safflower, pomegranate, kudzu, cherry, chrysanthemum, saffron, peach and rose.SELECTED DRAWING: Figure 1

The invention discloses a composition with immunoregulation and anti-aging functions and a preparation method of the composition. The composition comprises the following core components: marine collagen peptide, fusion polypeptide CPF-1, a monoclonal antibody mAb Senolix targeting aging cells, pentagalloylglucose (PGG) and hericium erinaceus polysaccharide. The fusion polypeptide CPF-1 is designed through a structural domain fusion strategy, and has collagen binding, immune activation and antibacterial functions; the monoclonal antibody mAb Senolix is targeted to a senescent cell surface antigen p16 (INK4a), and senescent cells are cleared through an ADCC effect. Through the synergistic effect of multiple components, the composition significantly improves the activity of immune cells, promotes collagen synthesis and alleviates chronic inflammation, shows excellent immunoregulation and anti-aging effects in an in-vitro cell model and an immunosuppressive mouse model, and can be used for developing immunopotentiators, anti-aging drugs or functional foods.

To provide a novel strain of acetic acid bacterium and its use or application, in response to ongoing investigation of the effects of acetic acid bacteria on fermentation performance and physiological functions, particularly regarding application of acetic acid bacteria to foods and health foods, and to meet the demand for employing previously unidentified acetic acid bacteria as fermentation-use microorganisms or immunoactivators.SOLUTION: An acetic acid bacterium identified as Gluconacetobacter takamatsuzukensis, deposited under deposit number NITE P-04018 with the Patent Microorganisms Depositary of the National Institute of Technology and Evaluation, and a fermentation composition containing the same are provided, and furthermore, compositions including immunoactivating compositions comprising the acetic acid bacterium are also provided.SELECTED DRAWING: Figure 1

The present invention relates to a composition for enhancing immunity, comprising, as an active ingredient, an enzyme-treated product of polysaccharides derived from a mixed extract of Angelica gigas, Cnidium officinale, and Paeonia lactiflora, or a novel purified fraction thereof. According to the present invention, the composition for enhancing immunity, comprising, as an active ingredient, an enzyme-treated product of polysaccharides from a mixed extract of Angelica gigas, Cnidium officinale, and Paeonia lactiflora, or a novel purified fraction thereof, comprises specific types and contents of sugars. Thus, when macrophages, splenocytes, and NK cells are treated with the composition, the composition significantly increases the activities thereof, and therefore can be applied as an immunostimulant or a food composition for enhancing immunity.

The invention discloses an immunogenic composition containing nanoparticles as well as a preparation method and application of the immunogenic composition. The immunogenic composition is prepared from self-assembled gE nano-particles, saponin QS-21 and a neutral liposome, wherein the self-assembled gE nano-particles are self-assembled gE nano-particles; the self-assembled gE nanoparticles are formed by polymerizing and assembling monomers; the monomer comprises a VZV gE, a connecting peptide 1 (SGS), a VZV gI polypeptide containing a Th epitope, a connecting peptide 2 (GSGSG) and an influenza virus hemagglutinin Th epitope. The immunogenic composition can be applied to VZV vaccines, and solves the technical problems of weak gE immunogenicity and serious vaccine side reaction in vaccines prepared in the prior art. The immunogenicity of the vaccine is higher than that of Xinanliei, the use of an immunopotentiator can be reduced, and the clinical side reaction is lower; the vaccine can also induce an obvious gI specific CMI reaction, which is beneficial to further improvement of the effectiveness of the vaccine. The immunogenic composition has good clinical application potential.

The invention belongs to the technical field of molecular biology, and relates to a truncated fish peptidoglycan recognition protein and application thereof. The amino acid sequence of the truncated fish peptidoglycan recognition protein is as shown in SEQ ID NO. 1 in the table. Compared with a natural peptidoglycan recognition protein, the truncated fish peptidoglycan recognition protein provided by the invention has higher expression efficiency and higher yield in a prokaryotic expression system; in addition, the binding capacity with main components, namely peptidoglycan (PGN) and lipopolysaccharide (LPS), of cell walls of gram-positive bacteria and gram-negative bacteria is higher; the bacteriostasis and agglutination capacities on gram-positive bacteria and gram-negative bacteria are obviously improved. The characteristics show that the truncated fish peptidoglycan recognition protein has the development potential as an aquatic product immunopotentiator, and important support is provided for subsequent application research.

The invention discloses an immunogenic composition as well as a preparation method and application thereof. The immunogenic composition comprises self-assembled gE nanoparticles, an immunopotentiator, namely, saponin QS-21, and a neutral liposome, the self-assembled gE nanoparticles are formed by polymerizing and assembling a plurality of monomers, and the monomers comprise VZV gE, a linker peptide (SGS) and VZV gI polypeptide containing Th epitopes. The immunogenic composition can be applied to VZV vaccines, and solves the technical problems of weak gE immunogenicity and serious vaccine side reaction in vaccines prepared in the prior art. The immunogenicity of the vaccine is equivalent to that of Xinanliei, and the use (variety and dosage) of an immunopotentiator can be reduced, so that the clinical side reaction of the vaccine is lower; in addition, the vaccine can induce a high-level gI specific CMI reaction, which is beneficial to further improvement of the effectiveness of the vaccine. In short, the immunogenic composition has good clinical application potential.

The invention provides an application of SpaA protein as an immunopotentiator in preparation of a vaccine for preventing viral subunits, the SpaA protein is swine erysipelas filamentous bacillus SpaA protein, and the viral subunit vaccine is a vaccine prepared from a virus shell special protein, namely an antigenic determinant. According to the swine erysipelas filamentous bacillus SpaA protein antigen and viral subunit protein combined vaccine serving as the immunopotentiator, the duration time of the blocking rate of the viral subunit protein can be remarkably prolonged or the neutralizing antibody and duration time of the viral subunit protein can be remarkably prolonged, and the onset time can also be shortened.

A pharmaceutical composition, a radiotherapy sensitizer, an immunopotentiator or an immunostimulatory agent comprising a metallosome comprising metal nanoparticles and a bacterial component attached thereto, and uses thereof in immunoregulation and tumor treatment are provided.

The invention discloses gE virus-like nanoparticles as well as a preparation method and application thereof. The gE virus-like nanoparticles are formed by polymerizing a plurality of monomers; the monomers include VZV gE and VZV gI polypeptides. The immunogenicity of the gE virus-like nanoparticle is better than that of gE, and the preparation method is simple; the gE antigen can be applied to VZV vaccines, and the technical problems that in vaccines prepared in the prior art, the immunogenicity of the gE antigen is poor, and side reactions of the vaccines are serious are solved. Compared with the Xinanlietaine, the vaccine taking the gE virus-like nanoparticles as the antigen has the advantages that the variety and dosage of the immunopotentiator are less, the immunogenicity is better, and the clinical side reaction is lower; besides, the gE virus-like nanoparticle vaccine not only can induce a gE specific antibody reaction, but also can induce a gI specific antibody reaction, so that the effectiveness of the vaccine is further improved. The gE virus-like nanoparticles have good clinical application potential.

The invention discloses a compound immunopotentiator as well as a preparation method and application thereof, and belongs to the technical field of aquaculture. The compound immunopotentiator contains a gallnut water extract, a gallnut alcohol extract, an astragalus membranaceus water extract, an astragalus membranaceus alcohol extract, a rhodiola rosea water extract, a rhodiola rosea alcohol extract and chlorogenic acid. The compound immunopotentiator provided by the invention can significantly improve the resistance of aquaculture animals to pathogen infection, is free of environmental pollution and drug resistance risks, and has good safety and effectiveness. Experiments show that for aquatic products infected with nervous necrosis viruses, the positive rate of the nervous necrosis viruses can be obviously reduced by adding the compound immunopotentiator into feed of the aquatic products; for aquatic products treated by mixed infection of nervous necrosis virus and vibrio harveyi, the death rate of the aquatic products can be obviously reduced by adding the compound immunopotentiator into feed of the aquatic products, so that the compound immunopotentiator disclosed by the invention has a good immunoprotection effect when coping with the mixed infection of virus and bacteria.

The invention discloses an immunogenic composition containing gE nanoparticles as well as a preparation method and application of the immunogenic composition, and relates to the technical field of biomedicine. The immunogenic composition is prepared from self-assembled gE nano-particles, saponin QS-21 and a neutral liposome, wherein the self-assembled gE nano-particles are self-assembled gE nano-particles; the self-assembled gE nanoparticles are formed by self-assembling monomers; the monomers comprise VZVgE, a connecting peptide and a VZV gI polypeptide containing a Th epitope. The immunogenic composition can be applied to VZV vaccines, and solves the technical problems of weak gE immunogenicity and serious vaccine side reaction in vaccines prepared in the prior art. The immunogenicity of the VZV vaccine is higher than that of Xinanliei, the dosage of an immunopotentiator is less, and the clinical side reaction of the vaccine is lower; in addition, the vaccine can also induce a gI specific CMI reaction, which is beneficial for further improving the effectiveness of the VZV vaccine. In short, the immunogenic composition has good clinical application potential.

The present invention relates to use of β-glucan extracts in the immunopotentiation of an avian animal. Specifically, the present invention provides a kit, wherein the kit comprises (1) an immunopotentiator used for immunopotentiation of an avian animal, wherein the immunopotentiator comprises a β-glucan extract which is extracted from Saccharomyces cerevisiae cell wall; and (2) a virus vaccine. The present invention also provides use of a β-glucan extract which is extracted from Saccharomyces cerevisiae cell wall, in the preparation of a kit used for immunopotentiation of an avian animal and immunopotentiator. The present invention uses a β-glucan extract, which is extracted from Saccharomyces cerevisiae cell wall, for immunopotentiation of an avian animal, which can enhance lymphocyte proliferation responses and T cell responses of vaccinated animals; decrease the viral load; reduce the morbidity or mortality; produce antibodies in advance; and increase antibody production; and / or restrict the spread of the virus.

The invention relates to the technical field of immune preparations, in particular to application of Forskolin or pharmaceutically acceptable salt thereof in preparation of a vaccine immunopotentiator. Aiming at the defects of vaccine immunomodulators in the prior art, the invention provides a novel method for enhancing the immune effect of a vaccine by using a natural plant extract Forskolin (as an adenylate cyclase activator), the Forskolin can promote high expression of CXCR4 by plasma cells to obviously enhance the homing ability, the residence ability and the service life of the plasma cells, so that the immune effect of the vaccine is enhanced. Therefore, the long-term immune response of the antibody is improved. The Forskolin directly targets the effector cytoplasm cells immunized by the vaccine, so that the potential safety hazard of the traditional technology is avoided, and meanwhile, more lasting and stable immune protection is provided.

The invention discloses application of rhein in preparation of a medicine for resisting iridovirus of micropterus salmoides. It is found and proved for the first time that rhein can be used as a fish feed additive, has good in-vivo safety, can be used as a drug for resisting LMBV infection of largemouth bass after being fed, has a good effect on resisting LMBV infection, can improve the survival rate of largemouth bass infected with LMBV, inhibits expression of virus in target tissue, and has good application prospects. Virus-induced fish target tissue structure damage is relieved, and the death rate caused by virus infection is reduced. Besides, as a disease-resistant immunopotentiator, the compound has the function of maintaining intestinal mucosa barrier, can maintain intestinal health of largemouth bass, can be better applied to prevention and control of largemouth bass iridovirus diseases, and can be used for preparing antiviral products.