30 results about "Echinoderm" patented technology

Methods for inducing systemic, non-specific and/or sequence specific immune responses in invertebrates, e.g., marine invertebrates such as mollusks, porifera, ctenophora, echinodermas, marine worms, cnideria and preferably crustaceans, by the administration of at least one dsRNA, that confers immunity against a pathogen, or modulates expression of gene that affects growth, reproduction, and general health or "robustness" are provided. Also provided are methods of identifying invertebrate genes, e.g., crustacean genes, the expression of which is involved in the induction of non-specific (systemic) immune responses against pathogens. Also disclosed are preferred delivery systems and methods for stably administering at least one dsRNA to a crustacean whereby the dsRNA is administered via injection, immersion, in a feed or nutrient medium or comprised in a microorganism, e.g., yeast or microalgae, that expresses said dsRNA and is ingestible by said crustacean, e.g., a shrimp.

In accordance with the invention, novel gene deletions and translocations involving chromosome 2 resulting in fusion proteins combining part of Anaplastic Lymphoma Kinase (ALK) kinase with part of a secondary protein have now been identified in human solid tumors, e.g. non-small cell lung carcinoma (NSCLC). Secondary proteins include Echinoderm Microtubule-Associated Protein-Like 4 (EML-4) and TRK-Fusion Gene (TFG). The EML4-ALK fusion protein, which retains ALK tyrosine kinase activity, was confirmed to drive the proliferation and survival of NSCLC characterized by this mutation. The invention therefore provides, in part, isolated polynucleotides and vectors encoding the disclosed mutant ALK kinase polypeptides, probes for detecting it, isolated mutant polypeptides, recombinant polypeptides, and reagents for detecting the fusion and truncated polypeptides. The disclosed identification of this new fusion protein enables new methods for determining the presence of these mutant ALK kinase polypeptides in a biological sample, methods for screening for compounds that inhibit the proteins, and methods for inhibiting the progression of a cancer characterized by the mutant polynucleotides or polypeptides, which are also provided by the invention.

The invention relates to an EML4-ALK (echinoderm microtubule associated protein like4-anaplastic lymphoma kinase) fusion gene fluorescent quantitative PCR (polymerase chain reaction) assay kit, which is applicable to assay of EML4-ALK fusion gene mutation in lung adenocarcinoma. The kit comprises probes, primers and positive controls, which are specially designed for conserved sequences of 9 fusion variations of the EML4-ALK fusion gene. The kit can be used for quickly and accurately assaying 9 most common EML4-ALK fusion gene variations with high sensitivity, namely 9 fusion variations of 7 variant subtypes, which are subtype 1 (E13; A20), subtype 2 (E20; A20), subtype 3 (E6a/b; A20), subtype 4 (E14; A20), subtype 5 (E2a/b; A20), subtype 6 (E18; A20) and subtype 7 (E14; A20), so that a real-time fluorescent quantitative PCR system for assaying 9 most common EML4-ALK fusion gene variations can be established.

The present invention discloses a process of extraction, purification and characterization of a non-polar fluorescent dye from a marine echinoderm Holothuria scabra, compositions containing the dye and various applications of the dye, said dye is a natural, cell permeant, nontoxic and environmentally friendly non-polar fluorescent dye.

The present invention discloses one kind of sea cucumber beer and its preparation process and identifying method, and features that the sea cucumber beer contains the active nutritious components of sea cucumber, including sea cucumber saponin in 10<-5> ppm to 1 %, and that the content of sea cucumber saponin in 0.3-60 ppm can results in excellent foaming performance and foam maintaining time as long as 6 hr. The sea cucumber beer is prepared in the basically same process as common beer, but the addition of water solution, alcohol solution and/or medicinal wine of sea cucumber before or after the fermentation. The water solution, alcohol solution and/or medicinal wine of sea cucumber as the material is identified via diluting for 2-200 with water for foaming identification.

In accordance with the invention, novel gene deletions and translocations involving chromosome 2 resulting in fusion proteins combining part of Anaplastic Lymphoma Kinase (ALK) kinase with part of a secondary protein have now been identified in human solid tumors, e.g. non-small cell lung carcinoma (NSCLC). Secondary proteins include Echinoderm Microtubule-Associated Protein-Like 4 (EML-4) and TRK-Fusion Gene (TFG). The EML4-ALK fusion protein, which retains ALK tyrosine kinase activity, was confirmed to drive the proliferation and survival of NSCLC characterized by this mutation. The invention therefore provides, in part, isolated polynucleotides and vectors encoding the disclosed mutant ALK kinase polypeptides, probes for detecting it, isolated mutant polypeptides, recombinant polypeptides, and reagents for detecting the fusion and truncated polypeptides. The disclosed identification of this new fusion protein enables new methods for determining the presence of these mutant ALK kinase polypeptides in a biological sample, methods for screening for compounds that inhibit the proteins, and methods for inhibiting the progression of a cancer characterized by the mutant polynucleotides or polypeptides, which are also provided by the invention.

The invention provides an individual marking method. According to the method, the problem that in traditional breeding, echinoderms are difficult to mark is solved, individual marking can be conveniently performed on the echinoderms such as sea cucumbers and sea urchins, the characters such as individual feed coefficients which cannot be recorded through traditional fluorescent marking can be also recorded besides that the individual growth characters can be monitored, and compared with traditional fluorescent marking, the method has the advantages that the cost is lower, and selected individuals can be more easily identified in young echinoderm selecting. Due to the method, the possibility of breeding the echinoderms through the BLUP method is achieved. Accordingly, the method has multiple advantages and is particularly suitable for being applied and popularized in the breeding field and very wide in market prospect.

The invention discloses an aquaculture device. The aquaculture device includes a culture box and tubular piles fixing the culture box; the culture box is formed by overlapping multiple net boxes, thetwo sides of each net box are both provided with a lantern ring, the lantern rings are connected with the tubular piles, the bottoms of the tubular piles are provided with pad supports connected withthe lantern rings, a bracket is arranged on the top of each tubular pile and connected with a feed pipe, one side of each feed pipe is provided with a feeding port, and the other side of each feed pipe is provided with multiple discharging ports connected with the net boxes. By adopting a multi-layer three-dimensional ecological culture mode, various fishes and echinoderms are cultured through thenet boxes, and culture at separated layers is achieved by utilizing life habits of the fishes and echinoderms; moreover, reasonable feeding can be conducted on the fishes and echinoderms cultured ateach layer through the feed pipes, baits are saved, the utilization rate of the baits is increased, the economic benefits are increased, and the culture rate of unit area is greatly increased.

The invention provides an application of Eucalyptus polybractea essential oil and its composite microbial agent in the preparation of a vermifuge for echinoderms. The Eucalyptus polybractea essentialoil composite microbial agent comprises Eucalyptus polybractea essential oil and Streptomyces griseus HL-50 with the bacterial content of 5 * 10<8> CFU/g or above, wherein the preservation number of the Streptomyces griseus HL-50 is CGMCC No.18424, and the most main effective component of the Eucalyptus polybractea essential oil is 1,8-cineole. The Eucalyptus polybractea essential oil composite microbial agent is simple to prepare and use, so effective repelling of various parasites infecting the echinoderms and bacterium inhibition can be achieved by only uniformly mixing an edible feed for the echinoderms with the composite microbial agent according to a ratio of 0.5-1.5 kg/t and continuously feeding the echinoderms with the obtained mixture for 4-7 d; and the composite microbial agent is nontoxic and harmless, is suitable for the echinoderms to use for a long term, can reduce the breeding cost, and has a wide application prospect.

The invention aims to provide an extract of echinodermata polar glycolipids and application thereof. The extract is used for preparing food drugs capable of promoting and improving neural differentiation, and has the effect of promoting the development of neural systems or preventing neural diseases. Sea cucumber, sea urchin and starfish polar glycolipids are extracted and purified, and act on neural cells; the results show that the extract of the three echinodermata polar glycolipids can obviously improve the differentiation rate of the neural cells and has an obvious effect of promoting and improving neural differentiation. Therefore, the extract of echinodermata polar glycolipids can serve as a compound or auxiliary material to prepare a product cooperatively. The preparation method of the extract of echinodermata polar glycolipids in the invention is low in cost and relatively simple in operation method; cell experiments show that the obtained extract of echinodermata polar glycolipids has a good effect of promoting neural development, and can be used for developing safe and effective food or drugs for improving the development of immature individual neural systems and preventing neurodegenerative diseases.

The invention provides a method of aquaculture comprising supplying Vibrio midae SY9 as a food source to farmed aquatic animals. A feed composition comprising Vibrio midae SY9 is also provided. The aquatic animals are typically marine animals which feed on algae. These include abalone and echinoderms, such as sea urchins, sea cucumbers, starfish, brittle stars, sand dollars and crinoids. The Vibrio midae SY9 can be provided on or within an alginate film formed on a support.