30 results about "Echinoderm" patented technology

Methods for inducing systemic, non-specific and / or sequence specific immune responses in invertebrates, e.g., marine invertebrates such as mollusks, porifera, ctenophora, echinodermas, marine worms, cnideria and preferably crustaceans, by the administration of at least one dsRNA, that confers immunity against a pathogen, or modulates expression of gene that affects growth, reproduction, and general health or "robustness" are provided. Also provided are methods of identifying invertebrate genes, e.g., crustacean genes, the expression of which is involved in the induction of non-specific (systemic) immune responses against pathogens. Also disclosed are preferred delivery systems and methods for stably administering at least one dsRNA to a crustacean whereby the dsRNA is administered via injection, immersion, in a feed or nutrient medium or comprised in a microorganism, e.g., yeast or microalgae, that expresses said dsRNA and is ingestible by said crustacean, e.g., a shrimp.

In accordance with the invention, novel gene deletions and translocations involving chromosome 2 resulting in fusion proteins combining part of Anaplastic Lymphoma Kinase (ALK) kinase with part of a secondary protein have now been identified in human solid tumors, e.g. non-small cell lung carcinoma (NSCLC). Secondary proteins include Echinoderm Microtubule-Associated Protein-Like 4 (EML-4) and TRK-Fusion Gene (TFG). The EML4-ALK fusion protein, which retains ALK tyrosine kinase activity, was confirmed to drive the proliferation and survival of NSCLC characterized by this mutation. The invention therefore provides, in part, isolated polynucleotides and vectors encoding the disclosed mutant ALK kinase polypeptides, probes for detecting it, isolated mutant polypeptides, recombinant polypeptides, and reagents for detecting the fusion and truncated polypeptides. The disclosed identification of this new fusion protein enables new methods for determining the presence of these mutant ALK kinase polypeptides in a biological sample, methods for screening for compounds that inhibit the proteins, and methods for inhibiting the progression of a cancer characterized by the mutant polynucleotides or polypeptides, which are also provided by the invention.

The invention relates to an EML4-ALK (echinoderm microtubule associated protein like4-anaplastic lymphoma kinase) fusion gene fluorescent quantitative PCR (polymerase chain reaction) assay kit, which is applicable to assay of EML4-ALK fusion gene mutation in lung adenocarcinoma. The kit comprises probes, primers and positive controls, which are specially designed for conserved sequences of 9 fusion variations of the EML4-ALK fusion gene. The kit can be used for quickly and accurately assaying 9 most common EML4-ALK fusion gene variations with high sensitivity, namely 9 fusion variations of 7 variant subtypes, which are subtype 1 (E13; A20), subtype 2 (E20; A20), subtype 3 (E6a / b; A20), subtype 4 (E14; A20), subtype 5 (E2a / b; A20), subtype 6 (E18; A20) and subtype 7 (E14; A20), so that a real-time fluorescent quantitative PCR system for assaying 9 most common EML4-ALK fusion gene variations can be established.

The present invention discloses a process of extraction, purification and characterization of a non-polar fluorescent dye from a marine echinoderm Holothuria scabra, compositions containing the dye and various applications of the dye, said dye is a natural, cell permeant, nontoxic and environmentally friendly non-polar fluorescent dye.

The present invention discloses a process of extraction, purification and characterization of a non-polar fluorescent dye from a marine echinoderm Holothuria scabra, compositions containing the dye and various applications of the dye, said dye is a natural, cell permeant, nontoxic and environmentally friendly non-polar fluorescent dye.

The present invention discloses the process of extraction, purification and characterization of a natural eco-friendly nontoxic cell permeant multiple fluorescent protein dye from a non bioluminescent marine echinoderm Holothuria scabra, compositions containing the dye and various applications of the dye.

The present invention discloses one kind of sea cucumber beer and its preparation process and identifying method, and features that the sea cucumber beer contains the active nutritious components of sea cucumber, including sea cucumber saponin in 10<-5> ppm to 1 %, and that the content of sea cucumber saponin in 0.3-60 ppm can results in excellent foaming performance and foam maintaining time as long as 6 hr. The sea cucumber beer is prepared in the basically same process as common beer, but the addition of water solution, alcohol solution and / or medicinal wine of sea cucumber before or after the fermentation. The water solution, alcohol solution and / or medicinal wine of sea cucumber as the material is identified via diluting for 2-200 with water for foaming identification.

In accordance with the invention, novel gene deletions and translocations involving chromosome 2 resulting in fusion proteins combining part of Anaplastic Lymphoma Kinase (ALK) kinase with part of a secondary protein have now been identified in human solid tumors, e.g. non-small cell lung carcinoma (NSCLC). Secondary proteins include Echinoderm Microtubule-Associated Protein-Like 4 (EML-4) and TRK-Fusion Gene (TFG). The EML4-ALK fusion protein, which retains ALK tyrosine kinase activity, was confirmed to drive the proliferation and survival of NSCLC characterized by this mutation. The invention therefore provides, in part, isolated polynucleotides and vectors encoding the disclosed mutant ALK kinase polypeptides, probes for detecting it, isolated mutant polypeptides, recombinant polypeptides, and reagents for detecting the fusion and truncated polypeptides. The disclosed identification of this new fusion protein enables new methods for determining the presence of these mutant ALK kinase polypeptides in a biological sample, methods for screening for compounds that inhibit the proteins, and methods for inhibiting the progression of a cancer characterized by the mutant polynucleotides or polypeptides, which are also provided by the invention.

The invention discloses a method for sampling and extracting DNA from living echinoderms. The tube podia of living sea urchins or tube podia, tentacles or intestines of living sea cucumbers are taken, lysed and digested with a lysate added with DNase, and extracted and centrifuged to obtain the DNA. Supernatant, centrifugal removal of slag (bone flakes), ethanol precipitation, centrifugal separation, detection and other steps make the concentration and quality of the extracted DNA consistent with the DNA extracted from the traditional (dead body) sampling tissue of echinoderms. It is not necessary to kill echinoderms, but samples are taken from living organisms, and then DNA is extracted, so that molecular biology techniques can truly become an auxiliary tool for genetic breeding. Utilizing the invention, the echinoderm resources can be rationally utilized to carry out auxiliary breeding, breeding status monitoring, gene screening, family identification and the like. In addition, it is of great significance to the protection and research of many endangered species of echinoderms.

Devices for guided tissue regeneration (GTR) include a matrix of chitosan and mutable collagenous tissue (MCT) wherein the chitosan is electrostatically bonded to the MCT to form MCT-chitosan composite material. The MCT can be isolated from invertebrate marine organisms, such as sponges, jellyfish, mollusks and echinoderms. The MCT-chitosan composite material can be formulated as a biofilm, a 3D-sponge, a hydrogel, or as an electrospun nanofiber, or the MCT-chitosan composite material can coat a biomaterial surface. The devices can include wound dressings and tissue sponges, including 3D sponges. Applications include tissue engineering and wound healing, as well as burns and other related guided tissue regeneration applications. MCT and MCT-chitosan composite material, contained in a pharmaceutically acceptable topical carrier, also has cosmeceutical applications, for treating scars, as well as skin discoloration and various pigmentation issues, including melasma / chloasma.

The invention provides an individual marking method. According to the method, the problem that in traditional breeding, echinoderms are difficult to mark is solved, individual marking can be conveniently performed on the echinoderms such as sea cucumbers and sea urchins, the characters such as individual feed coefficients which cannot be recorded through traditional fluorescent marking can be also recorded besides that the individual growth characters can be monitored, and compared with traditional fluorescent marking, the method has the advantages that the cost is lower, and selected individuals can be more easily identified in young echinoderm selecting. Due to the method, the possibility of breeding the echinoderms through the BLUP method is achieved. Accordingly, the method has multiple advantages and is particularly suitable for being applied and popularized in the breeding field and very wide in market prospect.

Methods for inducing systemic, non-specific and / or sequence specific immune responses in invertebrates, e.g., marine invertebrates such as mollusks, porifera, ctenophora, echinodermas, marine worms, cnideria and preferably crustaceans, by the administration of at least one dsRNA, that confers immunity against a pathogen, or modulates expression of gene that affects growth, reproduction, and general health or “robustness” are provided. Also provided are methods of identifying invertebrate genes, e.g., crustacean genes, the expression of which is involved in the induction of non-specific (systemic) immune responses against pathogens. Also disclosed are preferred delivery systems and methods for stably administering at least one dsRNA to a crustacean whereby the dsRNA is administered via injection, immersion, in a feed or nutrient medium or comprised in a microorganism, e.g., yeast or microalgae, that expresses said dsRNA and is ingestible by said crustacean, e.g., a shrimp.

The invention relates to an EML4-ALK (echinoderm microtubule associated protein like4-anaplastic lymphoma kinase) fusion gene fluorescent quantitative PCR (polymerase chain reaction) assay kit, which is applicable to assay of EML4-ALK fusion gene mutation in lung adenocarcinoma. The kit comprises probes, primers and positive controls, which are specially designed for conserved sequences of 9 fusion variations of the EML4-ALK fusion gene. The kit can be used for quickly and accurately assaying 9 most common EML4-ALK fusion gene variations with high sensitivity, namely 9 fusion variations of 7 variant subtypes, which are subtype 1 (E13; A20), subtype 2 (E20; A20), subtype 3 (E6a / b; A20), subtype 4 (E14; A20), subtype 5 (E2a / b; A20), subtype 6 (E18; A20) and subtype 7 (E14; A20), so that a real-time fluorescent quantitative PCR system for assaying 9 most common EML4-ALK fusion gene variations can be established.

The invention discloses a method for extracting larva DNA (Deoxyribonucleic Acid) before metamorphosis of echinoderm by a freezing method. The method comprises the following steps of: filling echinoderm larva in a centrifugal tube and preserving in a freezing mode under the condition of -80 DEG C; unfreezing and then adding 15 ul of cracking solution and 1 ul of 0.3 mg / ml protease K into the centrifugal tube in 10 minutes; cracking in a water bath for 3 hours and oscillating and shaking up once every half an hour, wherein the cracking solution comprises the following components with the following final concentration: 10 mmol / L Tris-Cl, 50 mmol / L of KCL and 0.5 percent of Tween-20, and the pH is 8.0; heating to 85 DEG C and processing in water bath for 15 minutes; and directly performing PCR (Polymerase Chain Reaction) amplification on a sample after cracking to obtain an amplified product, wherein a micro-satellite amplified PCR system consists of the following substances: 1 ul of DNA template, 2.5 ul of 10*buffer, 1.0 ul / each of primer, 2.2 ul of d NTP, 1.5 ul of MgCl2, 0.35 ul of Taq and 15.45 ul of dd H2O, 25 ul in total. The method is easy to operate and does not cause any harm to operators.

An Echinozoa tissue or organ extract comprising antioxidant compounds is disclosed. Also disclosed is a process for obtaining an Echinozoa tissue or organ extract, as well an extract obtained by this process. Compositions comprising such an extract are also described. Uses of such extracts / compositions, as well as corresponding methods of treatment, for example as an antioxidant or to decrease or inhibit oxidative stress in a cell, tissue or subject are also described.

The present invention discloses a process of extraction, purification and characterization of a natural eco-friendly nontoxic cell permeant multiple fluorescent protein dye from a non bioluminescent marine echinoderm Holothuria scabra, compositions containing the dye and various applications of the dye.

The invention discloses an aquaculture device. The aquaculture device includes a culture box and tubular piles fixing the culture box; the culture box is formed by overlapping multiple net boxes, thetwo sides of each net box are both provided with a lantern ring, the lantern rings are connected with the tubular piles, the bottoms of the tubular piles are provided with pad supports connected withthe lantern rings, a bracket is arranged on the top of each tubular pile and connected with a feed pipe, one side of each feed pipe is provided with a feeding port, and the other side of each feed pipe is provided with multiple discharging ports connected with the net boxes. By adopting a multi-layer three-dimensional ecological culture mode, various fishes and echinoderms are cultured through thenet boxes, and culture at separated layers is achieved by utilizing life habits of the fishes and echinoderms; moreover, reasonable feeding can be conducted on the fishes and echinoderms cultured ateach layer through the feed pipes, baits are saved, the utilization rate of the baits is increased, the economic benefits are increased, and the culture rate of unit area is greatly increased.

The invention provides an application of Eucalyptus polybractea essential oil and its composite microbial agent in the preparation of a vermifuge for echinoderms. The Eucalyptus polybractea essentialoil composite microbial agent comprises Eucalyptus polybractea essential oil and Streptomyces griseus HL-50 with the bacterial content of 5 * 10<8> CFU / g or above, wherein the preservation number of the Streptomyces griseus HL-50 is CGMCC No.18424, and the most main effective component of the Eucalyptus polybractea essential oil is 1,8-cineole. The Eucalyptus polybractea essential oil composite microbial agent is simple to prepare and use, so effective repelling of various parasites infecting the echinoderms and bacterium inhibition can be achieved by only uniformly mixing an edible feed for the echinoderms with the composite microbial agent according to a ratio of 0.5-1.5 kg / t and continuously feeding the echinoderms with the obtained mixture for 4-7 d; and the composite microbial agent is nontoxic and harmless, is suitable for the echinoderms to use for a long term, can reduce the breeding cost, and has a wide application prospect.

The invention discloses a method for sampling and extracting DNA from a living body of echinoderm. The tube feet of a living sea urchin or the tube feet, the tentacles or the intestines of a living sea cucumber are taken to be lysed and digested by lysis solution provided with DNA enzyme, and after the steps of extraction, centrifugal supernatant fluid taking, centrifugal deslagging (sclerite), ethyl alcohol precipitation, centrifugal separation, detection, etc., the concentration and the mass of the extracted DNA are consistent with the concentration and the mass of the DNA extracted from the traditional (dead body) sample tissue of the echinoderm. The echinoderm is not required to be killed, but is sampled from the living body to extract the DNA, so that the molecular biology technologycan really become an auxiliary mean of genetic breeding. By using the invention, the echinoderm resources can be rationally used for the works of auxiliary breeding, cultivation condition monitoring,genetic screening, genealogy identification, etc. In addition, the invention also has great significance for the protection and the research of various endangered species in the echinoderm.

The invention relates to protection development and effective use of shallow sea within 20 meters in the south China sea including offshore and high seas, and belongs to the technical field of echinoderm aquaculture of farm aquaculture, in particular to a tropic shallow sea Bohadschia argus bottom sowing reproducing method. Bottom sowing of the Bohadschia argusis is performed in a tropic ocean shallow sea area; bottom sowing breeding of the Bohadschia argusis can be performed by using environment resources in the area. The method can protect oceans, can continuously and effectively utilize the oceans, and can be duplicated in tropic ocean wide space.

Devices for guided tissue regeneration (GTR) include a matrix of chitosan and mutable collagenous tissue (MCT) wherein the chitosan is electrostatically bonded to the MCT to form MCT-chitosan composite material. The MCT can be isolated from invertebrate marine organisms, such as sponges, jellyfish, mollusks and echinoderms. The MCT-chitosan composite material can be formulated as a biofilm, a 3D-sponge, a hydrogel, or as an electrospun nanofiber, or the MCT-chitosan composite material can coat a biomaterial surface. The devices can include wound dressings and tissue sponges, including 3D sponges. Applications include tissue engineering and wound healing, as well as burns and other related guided tissue regeneration applications. MCT and MCT-chitosan composite material, contained in a pharmaceutically acceptable topical carrier, also has cosmeceutical applications, for treating scars, as well as skin discoloration and various pigmentation issues, including melasma / chloasma.

The invention discloses a method for preparing oligomeric fucoidan sulfate, which comprises the steps of using dry echinoderm as raw material, extracting supernatant by alkali hydrolysis and enzymolysis; adjusting the pH value of the supernatant to precipitate protein, centrifuging, salting Crude polysaccharides were obtained through ethanol precipitation and ethanol precipitation; fucoidan sulfate was obtained through ethanol fractional precipitation and ultrafiltration; the peroxide degradation catalyzed by the catalyst formed by the transition metal ion of the fourth period of the periodic table of elements or acid degradation was used to obtain oligomerization Fucoidan sulfate; Wherein, the weight-average molecular weight of described oligomeric fucoidan sulfate is 8-140kDa, and its structural characteristic is to comprise 20%-40% of the fucose of 40%-60% at least Sulfate groups.

The invention provides an application of Eucalyptus polybractea essential oil and its composite microbial agent in the preparation of a vermifuge for echinoderms. The Eucalyptus polybractea essentialoil composite microbial agent comprises Eucalyptus polybractea essential oil and Streptomyces griseus HL-50 with the bacterial content of 5 * 10<8> CFU / g or above, wherein the preservation number of the Streptomyces griseus HL-50 is CGMCC No.18424, and the most main effective component of the Eucalyptus polybractea essential oil is 1,8-cineole. The Eucalyptus polybractea essential oil composite microbial agent is simple to prepare and use, so effective repelling of various parasites infecting the echinoderms and bacterium inhibition can be achieved by only uniformly mixing an edible feed for the echinoderms with the composite microbial agent according to a ratio of 0.5-1.5 kg / t and continuously feeding the echinoderms with the obtained mixture for 4-7 d; and the composite microbial agent is nontoxic and harmless, is suitable for the echinoderms to use for a long term, can reduce the breeding cost, and has a wide application prospect.

The individual marking method of the present invention solves the problem that it is difficult to mark echinoderms in traditional breeding, and can conveniently make individual marks on echinoderms such as sea cucumbers and sea urchins. In addition to monitoring the growth traits of individuals, it can also record Traits that cannot be recorded using traditional fluorescent markers, such as the bait coefficient of individuals, are also cheaper than traditional fluorescent markers, and it is easier to identify selected individuals during seed selection. The emergence of this method makes it possible to breed echinoderms using the BLUP method. Therefore it can be said that it has possessed multiple advantages, and is particularly suitable for popularization and application in this field, and its market prospect is very broad.

The invention aims to provide an extract of echinodermata polar glycolipids and application thereof. The extract is used for preparing food drugs capable of promoting and improving neural differentiation, and has the effect of promoting the development of neural systems or preventing neural diseases. Sea cucumber, sea urchin and starfish polar glycolipids are extracted and purified, and act on neural cells; the results show that the extract of the three echinodermata polar glycolipids can obviously improve the differentiation rate of the neural cells and has an obvious effect of promoting and improving neural differentiation. Therefore, the extract of echinodermata polar glycolipids can serve as a compound or auxiliary material to prepare a product cooperatively. The preparation method of the extract of echinodermata polar glycolipids in the invention is low in cost and relatively simple in operation method; cell experiments show that the obtained extract of echinodermata polar glycolipids has a good effect of promoting neural development, and can be used for developing safe and effective food or drugs for improving the development of immature individual neural systems and preventing neurodegenerative diseases.

The present invention discloses one kind of sea cucumber beer and its preparation process and identifying method, and features that the sea cucumber beer contains the active nutritious components of sea cucumber, including sea cucumber saponin in 10<-5> ppm to 1 %, and that the content of sea cucumber saponin in 0.3-60 ppm can results in excellent foaming performance and foam maintaining time as long as 6 hr. The sea cucumber beer is prepared in the basically same process as common beer, but the addition of water solution, alcohol solution and / or medicinal wine of sea cucumber before or after the fermentation. The water solution, alcohol solution and / or medicinal wine of sea cucumber as the material is identified via diluting for 2-200 with water for foaming identification.

Devices for guided tissue regeneration (GTR) include a matrix of chitosan and mutable collagenous tissue (MCT) wherein the chitosan is electrostatically bonded to the MCT to form MCT-chitosan composite material. The MCT can be isolated from invertebrate marine organisms, such as sponges, jellyfish, mollusks and echinoderms. The MCT-chitosan composite material can be formulated as a biofilm, a 3D-sponge, a hydrogel, or as an electrospun nanofiber, or the MCT-chitosan composite material can coat a biomaterial surface. The devices can include wound dressings and tissue sponges, including 3D sponges. Applications include tissue engineering and wound healing, as well as burns and other related guided tissue regeneration applications. MCT and MCT-chitosan composite material, contained in a pharmaceutically acceptable topical carrier, also has cosmeceutical applications, for treating scars, as well as skin discoloration and various pigmentation issues, including melasma / chloasma.

The invention belongs to the technical field of agricultural insect pest control, and specifically relates to a biological control method for the pupal stage of the eucalyptus inchworm, which comprises the following steps: spreading metarhizium anisopliae granules during the pupation stage of the eucalyptus inchworms, the metarhizium anisopliae granules comprising: Metarhizium anisopliae spore powder and nutrients that provide nutrition for Metarhizium anisopliae. The beneficial effects of the present invention are: in the prevention and treatment method of the eucalyptus inchworm pupa stage, Metarhizium anisopliae has strong pathogenicity to Eucalyptus inchworm pupae, and the control effect is good; meanwhile, Metarhizium anisopliae is environmentally friendly, non-toxic and has no residue, and the Metarhizium anisopliae granules The agent contains nutrients, which can realize the continuous proliferation of Metarhizium anisopliae in the environment, maintain the amount of bacteria in the soil, and achieve the purpose of continuous pest control.

The invention provides a method for promoting maturation and artificial fertilization of starfish high-quality eggs, comprising: (1) cultivating starfish parents; (2) collecting starfish male and female gonad tissues; Guanine promotes the ripening of starfish eggs; (4) Artificial fertilization of starfish. The invention establishes the method for obtaining high-quality starfish ovum and artificial fertilization for the first time, and screens out the most suitable maturation-promoting agent and maturation-accelerating time for starfish ova through experiments. The invention has the characteristics of convenient operation, high efficiency and practicability, does not harm parent bodies, and the acquisition of ovum is not restricted by seasons; it has important application value and prospect of popularization and application in the aspects of echinoderm reproductive biology and experimental embryology research.

The invention provides a method of aquaculture comprising supplying Vibrio midae SY9 as a food source to farmed aquatic animals. A feed composition comprising Vibrio midae SY9 is also provided. The aquatic animals are typically marine animals which feed on algae. These include abalone and echinoderms, such as sea urchins, sea cucumbers, starfish, brittle stars, sand dollars and crinoids. The Vibrio midae SY9 can be provided on or within an alginate film formed on a support.