Polar glycolipid extract and application thereof

A polar glycolipid and extract technology, applied in the field of polar glycolipid extract, can solve the problem of slow development of marine active substances, and achieve the effects of increasing the differentiation rate of nerve cells, improving neural differentiation, and improving the neurodevelopment of children

Active Publication Date: 2016-09-07
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, due to geographical limitations, the development of marine active substances is slow, although in recent years there have been research reports that active substances isolated from echinoderms have

Method used

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  • Polar glycolipid extract and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: the preparation of sea cucumber lipid crude extract

[0019] The first step is to take the sea cucumber with internal organs removed, freeze-dry at -40°C, grind and sieve to obtain dry powder.

[0020] In the second step, 20 g of sample powder was weighed, leached once with chloroform / methanol (1:2, v / v), vortexed for 1 min and then allowed to stand for 10 min, and the upper layer extract was collected by centrifugation; the residue was washed with chloroform-methanol (3:1 , v / v) and then repeat the leaching for 2 to 3 times, combine the two supernatants and filter to obtain the leaching solution.

[0021] In the third step, add water to the extract and vortex it. After sufficient extraction, centrifuge to collect the upper methanol-water phase; add 0.01mol / L KCl solution and methanol (1:3, v / v) to the lower chloroform solution to extract again , combined the upper solution. Then the sample was loaded on an activated C8 solid-phase extraction column, elu...

Embodiment 2

[0022] Embodiment 2: Preparation of sea cucumber polar glycolipid extract

[0023] In the first step, the edible part of sea cucumber is taken, freeze-dried at -40°C, ground and sieved to obtain dry powder;

[0024] In the second step, 10 g of sample powder was weighed, leached once with chloroform / methanol (1:1, v / v), vortexed for 1 min and then allowed to stand for 10 min; the upper extract was collected by centrifugation, and the residue was washed with chloroform-methanol (1:1 , v / v) after repeated leaching for 2 to 3 times, the two supernatants were combined and filtered to obtain the leaching solution;

[0025] In the third step, add water to the extract and vortex it. After fully extracting, centrifuge to collect the upper methanol-water phase; add 0.01mol / L KCl solution and methanol (1:1, v / v) to the lower chloroform solution to extract again , combined the upper solution. Then load the sample on an activated C8 solid-phase extraction column, elute with methanol, and...

Embodiment 3

[0027] Embodiment 3: the preparation of sea urchin polar glycolipid extract

[0028] The first step is to take the edible part of the sea urchin, freeze-dry it at -40°C, grind it and sieve it to obtain a dry powder;

[0029] In the second step, weigh 40g of the sample powder, extract it once with chloroform / methanol (1:2, v / v), vortex for 1min and then let it stand for 10min; , v / v) after repeated leaching for 2 to 3 times, the two supernatants were combined and filtered to obtain the leaching solution;

[0030] In the third step, add water to the extract and vortex it. After sufficient extraction, centrifuge to collect the upper methanol-water phase; add 0.01mol / L KCl solution and methanol (1:3, v / v) to the lower chloroform solution to extract again , combined the upper solution. Then load the sample on an activated C8 solid-phase extraction column, elute with methanol, and carry out thin-layer silica gel plate chromatography (TLC) on the eluent, wherein the developing solv...

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Abstract

The invention aims to provide an extract of echinodermata polar glycolipids and application thereof. The extract is used for preparing food drugs capable of promoting and improving neural differentiation, and has the effect of promoting the development of neural systems or preventing neural diseases. Sea cucumber, sea urchin and starfish polar glycolipids are extracted and purified, and act on neural cells; the results show that the extract of the three echinodermata polar glycolipids can obviously improve the differentiation rate of the neural cells and has an obvious effect of promoting and improving neural differentiation. Therefore, the extract of echinodermata polar glycolipids can serve as a compound or auxiliary material to prepare a product cooperatively. The preparation method of the extract of echinodermata polar glycolipids in the invention is low in cost and relatively simple in operation method; cell experiments show that the obtained extract of echinodermata polar glycolipids has a good effect of promoting neural development, and can be used for developing safe and effective food or drugs for improving the development of immature individual neural systems and preventing neurodegenerative diseases.

Description

technical field [0001] The invention belongs to the technical field of active substance screening, and in particular relates to a polar glycolipid extract and its application. Background technique [0002] Nerve growth and differentiation is an important physiological phenomenon of human beings, which is not only related to the intellectual development in early childhood, but also related to neurodegenerative diseases such as Alzheimer's syndrome (AD), Parkinson's syndrome (PD), etc. closely related. Neural differentiation is characterized by axon formation, including neurite (including dendrites and axons) initiation, extension, branching, and formation of neural networks, which are crucial for the development of the nervous system and the regeneration of neurons after injury. Malfunctions in this process can cause a variety of diseases and conditions, including mental retardation, neurodegenerative diseases, and more. Therefore, normal nerve growth and differentiation ar...

Claims

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Application Information

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IPC IPC(8): A61K35/616A61P25/00C07H1/08A23L33/00
CPCA23V2002/00A61K35/616C07H1/08A23V2200/322A23V2250/2042
Inventor 徐杰王晓旭丛培旭薛长湖李兆杰曹建
Owner OCEAN UNIV OF CHINA
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