53 results about "Neural cell" patented technology

Disclosed herein are methods of treating ALS in a subject by administering to the subject a therapeutically effective amount of a composition comprising, for example, EV derived from nerve cells, such as neural progenitor cells. The EVs may be administered distally or peripherally to the CNS such that these EVs cross the blood-brain barrier and exert their therapeutic function in the CNS. The methods may reduce inflammation (e.g., NLRP3 inflammatory pathway signaling), reduce disease activity or progression, and / or improve motor or neurological performance, signs or symptoms associated with ALS, or survival in an ALS subject as compared to a control ALS subject. Also provided are methods of inhibiting necroptosis in a cell by contacting the cell with a therapeutically effective amount of a composition comprising EV.

The application belongs to the technical field of biological medical materials, and discloses a polydopamine modified modified nano-graphyne and a preparation method and application thereof. In the method, the polydopamine coating is combined with the nano-graphyne for the first time to construct a GDY@PDA composite system, and the core defects of the blocky graphyne, such as easy agglomeration, poor water solubility and insufficient biocompatibility, are solved. The graphyne-based material is applied to the near-infrared two-region photothermal regulation of neural cell calcium influx for the first time, the calcium ion channel is activated through the local thermal effect of the material, and precise, remote and reversible calcium influx regulation is realized; the limitation that the traditional biocompatibility modification is easy to fall off and difficult to functionalize is broken through, the polydopamine modification makes the material have both photothermal efficiency and biological safety, and the application of the graphyne in the treatment of neural diseases (such as neural cell injury repair) is expanded.

The invention relates to the technical field of nerve cell differentiation, and discloses a culture medium for promoting neural differentiation of SH-SY5Y cells. The culture medium disclosed by the invention is prepared from the following components: tazarotene (tazarotene) and a basic culture medium. The culture medium can promote SH-SY5Y cells to present neuron-like differentiation within 5 days, significantly improves the length of neurite and the expression level of neuron markers, and is low in cytotoxicity. The invention provides an efficient, safe and standardized new tool for neural disease in-vitro model construction, drug screening and mechanism research.

A method for assessing differentiation potential of cells in a culture broth in the differentiation of pluripotent stem cells into neural cells of the midbrain floor plate region, comprising: measuring a concentration of NT-3 in a culture supernatant in a culture broth obtained by culturing the pluripotent stem cells in a medium containing an inducer of differentiation into the neural cells of the midbrain floor plate region; comparing the measured concentration of NT-3 with a reference concentration; and when the concentration of NT-3 is equal to or more than the reference concentration, assessing the cells in the culture broth as capable of differentiating into the neural cells of the midbrain floor plate region, wherein the culture supernatant is a culture supernatant collected from the culture broth at any time from 48 hours to 240 hours after the start of culture of the pluripotent stem cells.

A method of activating a piezoelectric neuroconduit guide scaffold including a plurality of aligned piezoelectric polymer nanofibers, the method including mechanically stimulating the piezoelectric neuroconduit guide scaffold with hydro-acoustic waves or shockwaves to remotely activate a piezoelectric effect of the nanofibrous scaffolds that induces a mechano-electrical stimulus on neural cells cultured on the scaffold, wherein the mechano-electrical stimulus promotes nerve fiber outgrowth from the neuronal cells. Some aspects relate to seeding individual components of neural tissues on the piezoelectric neuroconduit guide scaffold, wherein the hydro-acoustic stimulation induces neural tissue formation. In other aspects, the piezoelectric neuroconduit guide scaffold is implanted in a damaged neural tissue, wherein stimulating the piezoelectric neuroconduit guide scaffold by the application of shockwaves promotes nerve fiber outgrowth that bridges a nerve gap to induce nerve regeneration or reinnervation of the damaged neural tissue.

The invention belongs to the technical field of biomedical materials, and discloses polydopamine modified nano graphdiyne as well as a preparation method and application thereof. According to the method, a polydopamine coating is combined with nano graphdiyne for the first time, a GDY and PDA composite system is constructed, and the core defects that blocky graphdiyne is prone to agglomeration, poor in water solubility and insufficient in biocompatibility are overcome. The graphdiynyl material is applied to near-infrared two-region photo-thermal regulation and control of nerve cell calcium internal flow for the first time, a calcium ion channel is activated through the local warming effect of the material, and accurate, remote and reversible calcium internal flow regulation and control are achieved; the limitation that traditional biocompatibility modification is easy to fall off and difficult to functionalize is broken through, the material has photo-thermal efficiency and biological safety through polydopamine modification, and the application of graphdiyne in nerve disease treatment (such as nerve cell damage repair) is expanded.

Disclosed are compositions and / or methods of use of the compositions for patients with neuronal diseases such as AD, Parkinson's, Huntington's, multiple sclerosis, and ALS. In certain embodiments flavonoids alone, or in a pharmaceutical preparation, are administered through the nasal olfactory route. In certain embodiments the flavonoid is apigenin and the neural disease is Alzheimer's. In some embodiments a porosome complex is administered for reconstitution into a neural cell. In certain embodiments, a co-administered blood-brain barrier traversing peptide is configured as a mimic of a domain of ATP 1 A3 and / or Tubulin.

The present invention relates to a method for producing neuronal cells from dedifferentiated adipocytes derived from a mammal, the method comprising a step for culturing dedifferentiated adipocytes derived from a mammal using a medium that contains a basic fibroblast growth factor (bFGF), retinoic acid, and a neuronal differentiation inducer, but does not substantially contain an epigenetic inhibitor.

The object of this invention is to provide a method of producing a nerve bundle including efficiently extending axons of neural cells. As a solution to accomplish this end, neural cells are cultivated in the presence of feeder cells including at least one type of cells selected from vascular component cells and perivascular cells.

An object of the present invention is to provide a method of evaluating intercellular interactions in neuroinflammation using a co-culture containing human-derived neural cells capable of mimicking human brain functions. According to the present invention, there is provided a method of evaluating intercellular interactions in neuroinflammation, the method including a step of producing a co-culture containing at least two cells selected from the group consisting of human-derived astrocytes, human-derived neurons, human-derived microglia, and human-derived oligodendrocytes, a step of applying an inflammatory stimulation to a first cell contained in the co-culture, a step of detecting at least one selected from the group consisting of an inflammatory response marker in the cells contained in the co-culture, neural activity of the cells, and cell morphology, and a step of evaluating, over time, a change in at least one selected from the group consisting of the inflammatory response marker, the neural activity, and the cell morphology in the first cell and a second cell different from the first cell contained in the co-culture.

The present application relates to the technical field of nano-preparation, in particular to an extracellular vesicle-liposome hybrid nanoparticle and a preparation method and application thereof. The extracellular vesicle-liposome hybrid nanoparticle provided by the present application significantly improves the ability of drugs to cross the blood-brain barrier and the target enrichment efficiency of ischemic lesions in the brain through active targeting modification; the specific response release of drugs is realized by using the high active oxygen microenvironment of the lesion, non-specific distribution of drugs is reduced, and the systemic toxic side effects are reduced; ultimately, a multi-target point synergistic treatment system capable of synchronously regulating neural cell apoptosis, glial cell inflammatory activation, brain microvascular endothelial cell ferroptosis and the structural integrity of the blood-brain barrier is constructed, the problem that the effect of traditional single target point intervention is limited is broken through from the root, and a more efficient, safe and precise new treatment strategy is provided for cerebral ischemia-reperfusion injury.

An antibody targeting an ATRN protein or an antigen-binding fragment thereof, and the use thereof, and belongs to the field of antibodies. The antibody or the antigen-binding fragment thereof comprises a heavy chain complementarity determining region HCDR, wherein the HCDR comprises at least one selected from amino acid sequences of SEQ ID NOs: 1-3 or an amino acid sequence having at least 80% homology thereto. The antibody or the antigen-binding fragment thereof is capable of binding to a human ATRN protein with high specificity and affinity, blocking the interaction thereof with receptors, inhibiting the signal transduction process, and restoring cell activity, thereby regulating obesity, melanin deposition, neural cell function and anti-tumor function of immune cells in organisms.

The application provides a neural cell spectrum detection method and system based on deep learning, and belongs to the technical field of spectrum analysis, and comprises the following steps: constructing and jointly training a spectrum feature extraction network, a spatial context aggregation network and an abundance prediction network; obtaining a hyperspectral microscopic image of a neural cell sample to be detected for preprocessing to obtain a hyperspectral data cube; inputting the hyperspectral data cube into the pre-trained spectrum feature extraction network to output a deep spectrum feature map of each pixel; inputting the deep spectrum feature map into the pre-trained spatial context aggregation network to output a spatial-spectrum joint feature map; and inputting the spatial-spectrum joint feature map into the pre-trained abundance prediction network to output an abundance map of the neural cell sample to be detected as an abundance detection result of the neural cell sample to be detected. The application solves the problem that the traditional method is difficult to accurately separate and quantitatively analyze the abundance distribution of each subcellular component.

The application relates to a construction method of a neural probe and array culture chip and a neural electrophysiological toxicity evaluation method and device, the construction method comprising the following steps: acquiring a base material, a probe material and a chip circuit layout; and processing the base material, the probe material and the chip circuit layout by adopting a traditional micro-electro-mechanical system manufacturing stripping process and a micro-pattern stamping technology to construct a chip base with an array neural probe; and constructing a multi-channel neural cell culture chamber corresponding to the array neural probe on the chip base to obtain a multi-channel chip with the neural probe and array culture. The construction method integrates an ultrathin neural probe array and an array culture chamber layer to form a complete multi-channel chip, the multi-channel chip can realize high-throughput neural electrophysiological toxicity detection, full-process automation and high-throughput of neural cell culture to toxicity detection can be realized on the multi-channel chip, and then test cost, time and complexity can be significantly reduced.

The application relates to a thymoma epithelial cell subpopulation with neuromuscular characteristics and application, and a thymoma epithelial cell subpopulation with neuromuscular characteristics is obtained through single clone dilution culture screening from a thymoma cell line Thy0517 of a patient with myasthenia gravis (MG) in combination, and a thymoma epithelial cell with neuromuscular characteristics in the cell subpopulation is named as Thymus_NMi; the cell subpopulation has synapse-like structures and neuromuscular adhesion characteristics, and efficiently expresses genes participating in neural cell adhesion and synapse connection, highly integrates neuromuscular double characteristics, and simulates key pathological characteristics of abnormal thymus-induced immune tolerance of MG patients in a molecular phenotype and physiological function, so that a cell model closest to a real clinical state is provided for exploring a myasthenia gravis occurrence mechanism.

The invention provides a quick response type nutrient composition for a cognitive load scene. The quick response type nutrient composition is prepared from the following raw materials in parts by weight: 400 to 600 parts of L-alpha-choline alfoscerate, 0.1 to 0.4 part of pyrroloquinoline quinone disodium salt, 4 to 6 parts of blueberry anthocyanin, 4 to 6 parts of gamma-aminobutyric acid, 4 to 6 parts of L-lysine and 4 to 6 parts of L-arginine. The invention further provides a preparation method and application of the quick response type nutrient composition for the cognitive load scene. Through the synergistic effect of the six components, a nerve cell energy metabolism-neurotransmitter balance-oxidation protection three-channel synergistic mechanism is formed, through the multi-target-point synergistic effect, the prepared nutrient composition has the multiple effects of improving the cognitive decline symptom, rapidly relieving cognitive fatigue, improving the thinking agility and reducing the error rate; the method is especially suitable for a high-intensity cognitive load scene. The invention belongs to the technical field of food processing.

This invention provides a method and system for spectral detection of neural cells based on deep learning, belonging to the field of spectral analysis technology. The method includes: constructing and jointly training a spectral feature extraction network, a spatial context aggregation network, and an abundance prediction network; acquiring hyperspectral microscopic images of the neural cell sample to be detected and preprocessing them to obtain a hyperspectral data cube; inputting the hyperspectral data cube into the pre-trained spectral feature extraction network to output a deep spectral feature map for each pixel; inputting the deep spectral feature map into the pre-trained spatial context aggregation network to output a spatial-spectral joint feature map; and inputting the spatial-spectral joint feature map into the pre-trained abundance prediction network to output an abundance map of the neural cell sample to be detected, which serves as the abundance detection result for the neural cell sample. This invention solves the problem that traditional methods struggle to accurately separate and quantitatively analyze the abundance distribution of various subcellular components.

The present disclosure pertains to universal and adoptable cell culture substrates designed for applications in biology, medicine, pharmaceutics, and other relevant fields. The disclosure particularly focuses on processes and methodologies for manufacturing such substrates, which prove advantageous for both non-neural (cells exhibiting robust adhesion and weak cell-cell junctions, as well as cells with regular adhesion and cell-cell junctions) and neural cell sheet engineering. These substrates aim to enhance cell adhesion, stimulate cell growth, and enable rapid and uniform harvesting of cell sheets. Additionally, the present invention introduces a scaffold-free method for tissue engineering, facilitating the creation of a 2D / 3D neural tissue construct featuring unidirectional neuron bundles.

The invention relates to the technical field of biological medicines, in particular to a recombinant E-type botulinum toxin precursor protein and application thereof. The recombinant E-type botulinum toxin precursor protein is prepared by utilizing a prokaryotic expression system, has complete structural conformation and biological function activity under the condition of not needing protease inception, and can be used for medical cosmetology or disease treatment. And the recombinant E-type botulinum toxin precursor protein single-chain molecule can form a crystal with a spherical structure, and has the characteristics of stability, safety and controllable quality. A novel tool molecule, a nerve cell delivery carrier and a candidate drug are provided for development and application of E-type botulinum toxin protein molecules, a basis is provided for further research and development of the functional effect of botulinum toxin and wider medical application, and the E-type botulinum toxin protein molecules have wide application prospects.

Methods for target, biomarker, and patient selection discovery in central nervous system disorders utilizing patient-derived cellular models, spatial proteomics, and machine learning. The method generates neural cells from forebrain regions from induced pluripotent stem cells, performs cell-type specific proteome profiling using antibody-enzyme conjugates and spatial proteome profiling, and applies statistical data augmentation to sparse biological datasets. Machine learning classifiers with SHAP-based feature importance identify ranked biomarkers from mass spectrometry data. The platform enables patient stratification by linking molecular signatures to symptom severity, drug screening through biomarker modulation, and diagnostic applications. Kits comprising antibodies for biomarkers including antibodies for biomarkers identified by the method facilitate implementation. Applications include autism spectrum disorder, rare neurodevelopmental disorders, schizophrenia, epilepsy, Alzheimer's disease, and Parkinson's disease.

The application relates to the field of protein engineering, and specifically discloses a neurosphere-inducing recombinant protein and a rapid induction culture method of a 3D neurosphere. The neurosphere-inducing recombinant protein is composed of the following functional domains: a silicon dioxide binding tag located at the N terminal of the neurosphere-inducing recombinant protein and used for specifically anchoring a glass substrate surface; an ECM-derived peptide multimer located at the C terminal of the neurosphere-inducing recombinant protein and used for mediating neural cell adhesion through integrin; and a rigid helix linker located between the silicon dioxide binding tag and the ECM-derived peptide multimer. Based on the neurosphere-inducing recombinant protein, neurons can be spontaneously and orderly aggregated at a lower seeding density under conventional culture conditions on a glass substrate to form three-dimensional neurospheres with clear boundaries and uniform sizes, and rapid, controllable and high-throughput induction formation of neurosphere bodies is realized.

The application discloses a medical image morphing registration method and system, and belongs to the technical field of medical image processing. The application aims to solve the problems of insufficient precision, topological structure damage and limited cross-scale modeling capability of existing registration methods when processing large-scale non-rigid deformation. The proposed method combines the semantic perception ability of the diffusion model and the local evolution characteristics of the neural cellular automaton. By constructing a multi-scale image pyramid, a conditional diffusion model is introduced at each scale to extract potential semantic scores, guiding the NCA module to iteratively model local regions and generate a deformation field that maintains topological consistency. Finally, through the layer-by-layer progressive method between scales, a high-precision non-rigid registration result is obtained.

Disclosed are compositions and / or methods of use of the compositions for patients with neuronal diseases such as AD, Parkinson's, Huntington's, multiple sclerosis, and ALS. In certain embodiments flavonoids alone, or in a pharmaceutical preparation, are administered through the nasal olfactory route. In certain embodiments the flavonoid is apigenin and the neural disease is Alzheimer's. In some embodiments a porosome complex is administered for reconstitution into a neural cell. In certain embodiments, a co-administered blood-brain barrier traversing peptide is configured as a mimic of a domain of ATP1A3 and / or Tubulin.

The invention discloses a culture medium and a method for obtaining an induced cortical-like neuron precursor cell cluster from iPSCs (induced pluripotent stem cells), and belongs to the technical field of biology. The culture medium comprises a neural stem cell induction liquid I and a neural stem cell induction liquid II, a single-layer adherent induction method is adopted, and iPSCs are efficiently differentiated into an induced cortical neuron precursor cell cluster through optimized inoculation density, matrix treatment and a staged induction process. The neural stem cells comprise neural stem cells, transition-state nerve cells and differentiated mature nerve cells (the cell type composition is highly similar to that of tissue-derived neural stem cells). The method overcomes the problems of complex induction process and high cell heterogeneity in a traditional embryoid body method, the induction efficiency can reach 100%, single cell sequencing analysis shows that all cells are nerve cells, the purity is 100%, and non-target cells are not detected. A uniform, stable and efficient cell tool is provided for brain development research, nervous system disease modeling and cell therapy.

The invention discloses a small molecule activator targeting nerve cell aromatase and application of the small molecule activator in cerebral ischemia injury, and belongs to the technical field of biological medicine. The activator can directly enhance the catalytic activity of aromatase without significantly affecting the gene transcription of aromatase, so that the local estrogen level in the brain is accurately improved, and the neuroprotection effect is achieved. The neuron aromatase is specifically activated, so that the side effect of systemic estrogen treatment is avoided. In-vivo and in-vitro experiments prove that the concentration of estrogen in a target region can be effectively improved through local intracerebral administration, and the survival rate of neurons in an oxygen-glucose deprivation / reoxygenation injury model is remarkably improved. The pharmaceutical composition provided by the invention is suitable for local administration or systemic administration of a central nervous system, can be combined with the existing therapy, and provides a brand new treatment strategy and drug candidate for cerebral ischemia injury.

To provide a method for freezing a cell aggregate containing nervous system cells.SOLUTION: A method for freezing a cell aggregate containing neural cells having a three-dimensional structure, the method comprising the steps of: (1) contacting the cell aggregate containing the neural cells having a three-dimensional structure with a preservation solution at 0°C or higher and 30°C or lower before freezing; A step of preparing a cell aggregate immersed in a preservation solution, and (2) a step of cooling and freezing the cell aggregate immersed in the preservation solution obtained in step (1) at least from a temperature about 5 °C higher than the freezing point of the preservation solution to a temperature about 5 °C lower than the freezing point at an average temperature decrease rate of 2 to 7 °C / min.SELECTED DRAWING: None

The invention relates to the technical field of marine organisms, in particular to a nucleic acid aptamer modified nerve extracellular vesicle and application thereof in prevention and treatment of marine guanamine neurotoxin poisoning. According to the invention, extracellular vesicles (nEVs) and a nucleic acid aptamer (Apt) of tetrodotoxin (TTX) are coupled to construct a guanamine toxoid nano antagonism system Apt-nEVs. According to the present invention, the Apt-nEVs retains the natural Na < + > channel on the nerve cell membrane, can synergistically provide the dual detoxification function of the coupling aptamer and the natural Na < + > channel to identify and trap TTX, can achieve the efficient detoxification of TTX in the non-cell system and the cell level, and can significantly protect the cell oxidative stress injury caused by TTX. In addition, the aptamer coupled extracellular vesicles (Apt-nEVs) obtained in the invention can also provide thought and reference for construction of a more broad-spectrum marine biotoxin antagonism system.

Disclosed herein are methods of treating ALS in a subject by administering a therapeutically effective amount of a composition comprising EVs, e.g., derived from neural cells, e.g., neural progenitor cells, to the subject. The EVs can be administered distally or peripherally to the CNS, such that the EVs cross the blood brain barrier and exert their therapeutic function in the CNS. The methods can reduce inflammation (e.g., NLRP3 inflammatory pathway signaling), reduce disease activity or progression, and / or improve motor or neurological performance, signs or symptoms associated with ALS, or survival in the ALS subject as compared to a control ALS subject. The methods of inhibiting necroptosis in a cell by contacting the cell with a therapeutically effective amount of a composition comprising EVs are also provided.

Disclosed are compositions and / or methods of use of the compositions for patients with neuronal diseases such as AD, Parkinson's, Huntington's, multiple sclerosis, and ALS. In certain embodiments flavonoids alone, or in a pharmaceutical preparation, are administered through the nasal olfactory route. In certain embodiments the flavonoid is apigenin and the neural disease is Alzheimer's. Targeted dosages may affect mitochondrial function in brain neural cells thus ameliorating a neural disease. In some embodiments a porosome complex is administered for reconstitution into a neural cell. In certain embodiments, one or more macrocyclic or linear peptides are administered.

Meta-training an artificial neural cell for use in a few-shot learner, wherein the meta-training includes: executing a Neural Architecture Search (NAS) to automatically learn an architecture of the artificial neural cell; training adaptive controllers that are comprised in the architecture of the artificial neural cell, wherein each of the adaptive controllers is configured to adapt the architecture of the artificial neural cell to a few-shot learning task; and regressing the architecture of the artificial neural cell from support data of the few-shot learning task, through the adaptive controllers. Generating the few-shot learner based on the meta-trained artificial neural cell, to form an Artificial Neural Network (ANN).