215 results about "Tetanus" patented technology

The disclosure relates to cancer ribonucleic acid (RNA) vaccines, as well as methods of using the vaccines and compositions comprising the vaccines. In particular, the disclosure relates to concatemeric mRNA cancer vaccines encoding several cancer epitopes on a single mRNA construct, i.e. poly-epitope mRNA constructs or poly-neo-epitope constructs. The disclosure further relates to p53 and KRAS mutations, as well as incorporation of immune enhancers such as STING, e.g. mRNA constructs further encoding an immune stimulator or adjuvant. The disclosure further relates to inclusion of universal T cell epitopes, such as tetanus or diphtheria toxins to elicit an enhanced immune response.

Disclosed are vaccines and vaccine adjuvants useful in the treatment and/or prevention of infection and diseases associated with infectious pathogens, such as tetanus, as well as diseases associated with biological toxins. Also provided are methods of preparing an adjuvant and the vaccine containing the adjuvant. Methods are also provided for vaccinating/immunizing an animal against infection and diseases associated with infectious pathogens, such as tetanus, and other diseases associated with biological toxins. Adjuvant materials are presented that are prepared from an extracellular matrix material. The adjuvants are demonstrated to enhance the immunogencity of an infectious pathogen antigen or biological toxin antigen of interest, as well as to enhance the survival of an immunized animal.

The invention relates to a Chinese medicine preparation for curing tetanus. The formula includes earthworm, cicada ecdysis, rhizoma gastrodiae, notopterygium root, parsnip, herba schizonepetae, rhizoma arisaematis, uncaria, red peony, alum, ophiopogon japonicus, cortex moutan, Indian bread with hostwood, root poria, centipede, whole worm, atrina glass, honeysuckle, hemlock parsley, forsythia, safflower, peach seed and angelica. In the formula, the raw materials are pulverized, filtered and then made into pulvis. During the medicine taking, the medicine is mixed with cold boiled water, with two or three times per day and three days is one treatment period. The Chinese medicine preparation, compared with the prior art, is scientific and reasonable in the formula, simple in the regrouping ofthe raw materials and preparation process, effective in reducing the treatment cost and remarkable in treating tetanus. Having no toxic side effects to the human body, the invention is the medicine prepared commonly in homes.

The invention discloses an anti-tetanotoxin antibody. In the heavy chain variable region, the amino acid sequence of CDR1 is disclosed as SEQ ID NO.5, the amino acid sequence of CDR2 is disclosed as SEQ ID NO.6, and the amino acid sequence of CDR3 is disclosed as SEQ ID NO.7; and in the light chain variable region, the amino acid sequence of CDR1 is disclosed as SEQ ID NO.8, the amino acid sequence of CDR2 is disclosed as SEQ ID NO.9, and the amino acid sequence of CDR3 is disclosed as SEQ ID NO.10. The invention also discloses a nucleotide sequence for coding the anti-tetanotoxin antibody, and a corresponding recombinant plasmid and recombinant expression vector. The invention also discloses a preparation method and application of the anti-tetanotoxin antibody. The anti-tetanotoxin antibody disclosed by the invention is a full human antibody, has the advantages of low side reaction, high affinity and simple preparation method, and has wide industrial application prospects.

The invention discloses a preparation method of an ultramicro gastrodia elata decoction piece. The preparation method comprises selection of raw materials, cleaning of the raw materials, boiling, slicing, secondary boiling, drying, ultramicro wall breaking, obtaining of a preparation, and the like. Gastrodia elata processed by employing the processing and ultramicro fragmentation method has relatively high effective-composition dissolution rate, the bioavailability of a medicine is improved, the medicine effect is relatively good, and the toxicity is relatively low. The invention also discloses the decoction piece prepared by employing the method, and application of the decoction piece to prepare medicines for treating headache dizziness, numbness of limbs, infantile convulsion, epilepsy, twitch, tetanus and other diseases.

The present invention relates to a combination vaccine comprising a mixture of antigens for protection against diseases such as diphtheria, tetanus, acellular pertussis, and infections caused by Haemophilus influenzae and polio viruses. The present invention also relates to inclusion of antigens for protection against infections caused Hepatitis virus and other pathogens, such that administration of the vaccine can simultaneously immunize a subject against more than one pathogen. The invention in particular relates to a fully liquid stable combination vaccine comprising the antigens as indicated above and the methods for manufacturing the same.

The invention discloses a traditional Chinese medicine preparation for treating tetanus, which comprises the following raw material medicaments in parts by weight: 15 parts of taraxacum, 15 parts of fish flabellate, 15 parts of exuviae cicada, 15 parts of scorpio, 8 parts of white silkworm, 8 parts of gastrodia, 8 parts of rhizoma arisaematis, 8 parts of hurricane lamp, 8 parts of angelica, 8 parts of herba schizonepetae, 8 parts of notopterygium root and 8 parts of annulus enclosure. The preparation comprises a pill, a powdery preparation, a tablet, an oral liquid preparation and a capsule prepared by a traditional preparation technology or a modern preparation technology. The adopted medical materials have the efficacy of clearing away heat and toxic material, removing carbuncle and eliminating stagnation, dispersing stagnated liver for regulating qi, promoting blood circulation for removing blood stasis, and relieving exterior syndrome for dispersing cold. The traditional Chinese medicine preparation enhances the immune function of an organism by oral administration, has good treating effect on the tetanus caused by various diseases, and is convenient to take. Clinical application proves that the traditional Chinese medicine preparation has the advantages of quick action, obvious treating effect, good durability, low cost and no any toxic or side effect.

The invention belongs to the technical field of lateral side stream chromatography and bioanalysis, particularly relates to a test strip for detecting tetanus antibody on basis of quantum dot fluorescent microsphere chromatography and a preparation method and using method thereof, and aims at solving the technical problems in the process of rapidly and portably detecting tetanus antibody content via low consumption. The preparation method of the test strip comprises the following steps of: firstly closing a sample pad by using solution with a TW20 mass concentration of 0.5-5%; marking a detection line and a quality control line on an NC membrane in sequence; drying the processed sample pad and the NC membrane at 37 DEG C for 1 hour; pasting the sample pad, the NC membrane and a water absorption pad on a PVC baseboard in sequence; carrying out overlapping in pairs to 2-3 mm; and finally cutting into 3.9 mm wide to obtain the finished test strip. Compared with traditional colloidal goldimmunochromatography test strips, the test strip is good in quantum dot microsphere marker stability and high in detection sensitivity, and is capable of realizing quantitative detection for low-concentration target objects.

The invention relates to the filed of pharmaceutical preparation, in particular to single dose immunization against tetanus toxin cation dextran microspheres and a preparation method thereof. The single dose immunization against tetanus toxin cation dextran microspheres are prepared by carrying tetanus toxin after electrostatic interaction on cation hydroxyethyl acrylate dextran microspheres. The tetanus toxin controlled release microspheres can reduce the injection frequency of tetanus vaccine, improve the vaccination coverage and reduce the drop-out rate, thereby effectively preventing tetanus and providing a single dose tetanus toxin controlled release vaccine preparation with long-term effect and realizing the whole course immunity by one injection.

The invention relates to a tetanus treatment traditional Chinese medicine preparation, which is prepared from the following bulk drugs, by weight, 13-17 parts of Chinese bastardtoadflax herb, 13-17 parts of fragrant solomonseal rhizome, 13-17 parts of paniculate bolbostemma bulb, 13-17 parts of white peony root, 10-14 parts of milkvetch root, 10-14 parts of unprocessed rehmannia root, 10-14 parts of asiatic plantain herb, 9-11 parts of angelica, 9-11 parts of centipede, 9-11 parts of stiff silkworm, 6-8 parts of sharpleaf uncaria stem with hooks, 6-8 parts of giant typhonium tuber, 6-8 parts of divaricate saposhnikovia, 4-6 parts of scorpion, and 4-6 parts of reddish jackinthepulpit tuber. The tetanus treatment traditional Chinese medicine preparation has advantages of significant treatment effect, rapid effect and low toxic-side effect, wherein clinical trial results show that tetanus treatment efficiency is 96%.

The invention discloses a neutralizing monoclonal antibody resisting to tetanus toxin. Hybridoma cells are obtained from tetanus toxin heavy chain C fragment immune mice, light chain and heavy chain variable region genes of the antibody are taken, human-mouse chimeric complete antibody expression vectors are built, CHO cell transfection is carried out, purification is carried out, and then the antibody is obtained. The antibody has the activity of specific binding to tetanus toxin, the monoclonal antibody can partially protect mice against attack of tetanus toxin, and four antibodies can completely protect mice against a twofold lethal dose of tetanus toxin in combination.

The ointment for healing traumatic injury fast is prepared with 21 kinds of Chinese medicinal materials including ledebouriella root, dahurian angelica root, ground beetle, dragon's blood, cimicifugarhizome, etc. The preparation process includes soaking, drying, grinding into fine powder, sieving, mixing, bottling and other steps. When used, it is mixed with proper amount of turpentine, Vaselineor spirit to produce ointment. It has the integrated functions of stopping pain, stopping bleeding, relaxing muscles and joints, dispersing bloood clots, elimianting swelling, expelling wind and wetness, promoting healing of fracture and preventing tetanus and has no toxic side effect.

The invention discloses a hydrophobia-tetanus double titer human immunoglobulin, wherein the purity of the immunoglobulin is more than or equal to 90 percent of total protein, the titer of the hydrophobia neutralizing antibody is more than or equal to 100IU/mL, the titer of the tetanus neutralizing antibody is more than or equal to 40IU/mL, and the content of protein is less than or equal to 180g/L. In addition, the invention also discloses a method for preparing the hydrophobia-tetanus double titer human immunoglobulin and application of the hydrophobia-tetanus double titer human immunoglobulin in preparing medicaments for preventing hydrophobia and tetanus.

The invention discloses a completely humanized monoclonal neutralizing antibody for a tetanus toxin. The completely humanized anti-tetanus toxin neutralizing antibody is developed via a comprehensivehigh-throughput completely humanized monoclonal antibody research and development technical platform. The neutralizing antibody is high in affinity and specificity and has an important significance onprevention and treatment of tetanus infection.

The invention discloses a Chinese herba preparation for treating tetanus. The selected crude drugs comprise the following components by weight: 20-30g of nam sing, 10-20g of gastrodia elata, 10-20g of periostracum cicada, 10-20g of bezoar, 10-20g of notopterygium incisium, 10-20g of pawpaw, 10-20g of cassia twig, 10-20g of tribulusterrestris, 10-20g of centipede, 8-15g of fructus evodiae, 8-12g of antelope horn, 8-12g of tabasheer, 8-12g of dandelion, 8-12g of coptis chinensis and 8-12g of astragalus membranaceus. The medicines are of good compatibility, plays the functions of supporting right and banking up the root, tranquilizing the mind, carrying out spasmolysis, activating blood, promoting the circulation of qi, perfusing meridian, relieving cough and eliminating phlegm. Clinical application finds that the Chinese herba preparation has the advantages of rapid effect taking, short treatment course, no side effect and high cure rate for treating tetanus, and is worthy of clinic promotion and application.

The invention discloses a Chinese herbal medicine for treating tetanus, which relates to a medicament preparation product and aims to increase a medicine variety for treating the tetanus. The Chinese herbal medicine comprises the following raw materials: 0.5-4g of climbing groundsel herb, 2-25g of Alangium platanifolium, 1-10g of serissa and 0.5-5g of wild chrysanthemum flower. The preparation method comprises the following steps of: cleaning the climbing groundsel herb, the Alangium platanifolium, the serissa and the wild chrysanthemum flower, mincing the raw materials and drying, crushing and milling, evenly mixing according to mass composition of each pharmaceutical formulation, and sealing and packaging and storing in a cool and dry place to obtain the medicine for later use. The usage method comprises the steps of boiling the medicine by adding with water, fumigating infected parts through steam, and washing the infected parts by warm medicinal water. The Chinese herbal medicine has the functions of clearing away heat and toxic materials, inflammation diminishing and swelling reducing, detoxifying, and being capable of restraining and killing clostridium tetani vitality. The raw materials are easily obtained, the cost is low, the preparation process is simple, and the medicine is suitable for patients of the tetanus.

The present invention describes a neurotoxin associated protein from botulinum neurotoxin complex used as an oral or nasal delivery system for a vaccine. The vaccine is selected from tetanus, diphtheria and pertussis alone or in combination. Further the oral or nasal delivery of tetanus vaccine in combination with other drug molecules.

The invention discloses a medicine for treating tetanus and a preparation method thereof. The medicine is mainly prepared from the following components in a certain weight ratio: periostracum cicada, rhizoma typhonii, rhizoma arisaematis, gentiana macrophylla, gastrodia elata, ramulus mori, honeysuckle, rheum officinale and schizonepeta. The medicine has functions of removing dampness to reduce phlegm, dispelling wind-evil to calm nerves and eliminating swelling to remove stasis. The medicine which is used for treating the tetanus is rapid in efficacy and good in treatment effect without relapse.