95 results about "Tetanus" patented technology

The invention provides a chemically modified tetanus toxin C fragment as well as preparation and application thereof. The method for chemically modifying the tetanus toxin C fragment comprises the following steps: 1) reacting the tetanus toxin C fragment with a reducing agent to reduce a disulfide bond in the tetanus toxin C fragment; and 2) carrying out a reaction on the reduced tetanus toxin C fragment and an alkylation reagent, and carrying out modification. The recombinant tetanus toxin C fragment prepared by the method disclosed by the invention not only meets the quality standard required by pharmacopeia, but also has the advantages of stable conformation, high yield, easiness in amplification and low cost, and has a wide application prospect in the preparation of human vaccines, veterinary vaccines and antibody drugs and the development process of in-vitro diagnostic reagents.

The invention provides an application of an anti-tetanus toxin antibody in prevention or treatment of tetanus. The anti-tetanus toxin antibody is applied according to a specific administration scheme, and the technical effects that the anti-tetanus toxin antibody takes effect more quickly and the protection time is longer than that of an existing passive immune preparation can be achieved, so that tetanus can be effectively prevented or treated.

The present invention provides a pharmaceutical combination comprising a tetanus-adsorbing vaccine and an antibody against tetanus toxin or an antigen-binding fragment thereof. The pharmaceutical composition is used for preventing and / or treating tetanus. According to the present invention, the combination of the recombinant natural completely humanized monoclonal antibody and the tetanus adsorption vaccine proves that the detection time of the antibody induced by the tetanus adsorption vaccine is advanced, and the high titer level can last for a longer time;

The disclosure relates to cancer ribonucleic acid (RNA) vaccines, as well as methods of using the vaccines and compositions comprising the vaccines. In particular, the disclosure relates to concatemeric mRNA cancer vaccines encoding several cancer epitopes on a single mRNA construct, i.e. poly-epitope mRNA constructs or poly-neo-epitope constructs. The disclosure further relates to p53 and KRAS mutations, as well as incorporation of immune enhancers such as STING, e.g. mRNA constructs further encoding an immune stimulator or adjuvant. The disclosure further relates to inclusion of universal T cell epitopes, such as tetanus or diphtheria toxins to elicit an enhanced immune response.

The present disclosure provides an anti-tetanus toxin neutralizing antibody as a new generation of tetanus passive immune formulations. The antibody can be completely human-derived, can be specifically combined with the full length of tetanus toxin and AB fragments thereof, has the characteristics of high affinity, high specificity and small toxic and side effects, avoids the problems of large adverse reaction and short half-life period of animal-derived monoclonal antibodies, can be prepared in a large scale through mammalian cells, and has clear and stable components.

The invention relates to tetanus subtoxin and a preparation method thereof. The preparation method specifically comprises the following steps: providing or constructing a tetanus toxin Hc fragment mutant TeNT-Hc strain; inoculating the TeNT-Hc strain into a seed culture medium according to an inoculum size of 0.1%-0.5%, and carrying out shake culture until the OD600 value reaches 0.8-1.5 to obtain a first-stage seed culture solution; further increasing the number of strains on the basis of the first-stage seeds to obtain a second-stage seed culture solution; performing fermentation; re-suspending, crushing and centrifuging the bacteria, collecting supernate, carrying out Ni column affinity chromatography on the supernate, washing impurities and eluting, and collecting eluent; and carrying out second-step purification on the eluent through isoelectric precipitation or Q anion exchange or SP ion exchange, and collecting the supernate or the eluent to obtain the tetanus subtoxin. The purity of 95% or above is achieved through two-step purification, and meanwhile the expression quantity of the target protein is increased.

The invention relates to the technical field of biological medicine, and discloses a titer determination method of a specific immune globulin neutralizing antibody, which comprises the following steps: step 1, preparing a cell maintenance fluid; 2, preparation of a virus solution for neutralization: diluting viruses (cytomegalovirus, syncytial virus and EV71 virus) to 100CCID50 by using a diluent according to the virus titer; step 3, diluting the test sample: carrying out double series dilution on the intravenous injection human immune globulin, the tetanus human immune globulin, the rabies human immune globulin and the human immune globulin until no cytotoxicity exists; 4, diluting a standard substance; 5, virus back dripping; step 6, neutralizing viruses; step 7, preparing cells for the test; step 8, infection; and 9, judging a result. According to the present invention, the accurate detection on the titer of the neutralizing antibody of the specific immune globulin can be achieved, and the cytotoxicity influence of the test sample and the fringe effect during the detection process can be removed so as to ensure the detection accuracy.

The invention provides a pertussis (five components), diphtheria and tetanus combined vaccine, and particularly relates to a pertussis and tetanus combined vaccine with improved immune response. The immunogenic composition and the vaccine provided by the invention contain specially selected double aluminum-based adjuvants, so that the prepared composition vaccine can induce an organism to generate a good immune response, and meanwhile, the safety of the vaccine is ensured.

The present invention describes a second-generation tetanus toxoid vaccine and a process for the preparation thereof, comprising the steps of: inducing an E. coli culture OD600=0.5 by adding 0.2 mM IPTG; growing the culture at 14-16° C. for 14 to 20 hours; suspending the culture in 25 mM phosphate buffer containing 200 mM sodium chloride; adding 1% of triton-X-100 to the phosphate buffer, and adding the buffer to the culture; sonicating the culture for a period of 3 minutes (at 5 sec on / off pulse) at 4° C. on cold beads; centrifuging the culture for 60 to 90 minutes; collecting and purifying a supernatant using Ni-NTA affinity column with an eluant; and combining the supernatant into a pool with contaminated bands and concentrating using Centriprep-30 centrifuge filters (30 kDa pores).

1. Name of this design product: Skin Test Judgment Ruler. 2. Application of this design: This design is used for skin tests of penicillin, cephalosporins, tetanus and tuberculin. 3. The key design feature of this product is its pattern. 4. The image or photograph that best illustrates the design's key features: the front view. 5. This design is a thin product, so the left view is omitted; this design is a thin product, so the right view is omitted; this design is a thin product, so the top view is omitted; this design is a thin product, so the bottom view is omitted.

The utility model discloses a tetanus fermentation tank tail gas treatment device which comprises a fermentation tank, a tail gas discharge pipeline of the fermentation tank is provided with a one-way valve, and the fermentation tank is connected with a waste gas absorption alkali barrel through the tail gas discharge pipeline; the tail end of the tail gas discharge pipeline is connected with a gas inlet pipeline; and the gas inlet pipeline is positioned in the waste gas absorption alkali barrel. According to the mode, the harm caused by hydrogen sulfide in tail gas emission of a broken fermentation tank can be solved, the hydrogen sulfide in the tail gas is fully absorbed by a large amount of alkali liquor, the situation that odor and toxic gas appear in an indoor working place and harm health of people is avoided, and meanwhile high energy cost and safety risks are avoided.

The plasma mixing device for tetanus human immune globulin comprises a bottom plate, a sliding table is slidably connected to the top of the bottom plate, a driving assembly is arranged in the sliding table, a supporting rod is arranged at the top of the driving assembly and rotatably connected with the sliding table, a guiding assembly is arranged at the top of the supporting rod, and the guiding assembly is rotatably connected with the sliding table. A transmission rod is arranged at the top of the guide assembly, a supporting table is rotationally connected to the outer side of the transmission rod, the bottom of the supporting table is fixedly connected with the top of the sliding table, a cylinder is fixedly connected to the top of the transmission rod, a screw cap is in threaded connection with the top of the cylinder, and a disc is arranged on the rear side of the driving assembly. A cylindrical rod is arranged on the rear side of the disc, a limiting frame is connected to the rear side of the cylindrical rod in a sliding mode, and the problems that a simple stirring or shaking mode is mostly adopted in an existing uniform mixing device and method, the efficiency of the mode is low, uneven distribution of components in plasma is easily caused, and the quality and the effect of a final product are affected are effectively solved.

The invention discloses a recombinant tetanus toxin protein rTT as well as a preparation method and application thereof, the recombinant protein sequentially contains a His tag, a modified exogenous polypeptide and two tetanus toxin fragments connected in series through flexible polypeptides from the N end to the C end, the two tetanus toxin fragments connected in series form two intramolecular disulfide bonds in a molecule through double mutation, and the tetanus toxin fragments are connected in series through flexible polypeptides. Coding nucleotide of the recombinant protein is expressed by escherichia coli BL21 (DE3), induced at low temperature and purified, and target protein with the purity larger than or equal to 95% can be obtained. A subunit vaccine prepared from the protein and a two-way oil adjuvant ISA201 enables mice to achieve 100% protection under 100 LD50 challenge dose, the protection effect is better than that of truncated tetanus toxin heavy chain fragment TTc protein, and the recombinant protein can be used for tetanus prevention vaccines and serological diagnostic reagents.

Disclosed in the present invention is a pharmaceutical combination comprising an adsorbed tetanus vaccine and an antibody or an antigen-binding fragment thereof against tetanus toxin. The pharmaceutical combination is used for preventing and / or treating tetanus.

The present invention relates to a nucleic acid molecule for modulating neuromuscular transmission, said nucleic acid molecule comprising a sequence encoding one or more motor endplate receptors or fragments thereof. The present invention further relates to a fusion protein, preferably comprising one or more features of one or more of the foregoing Claims, said fusion protein comprising a motor endplate receptor and / or fragments thereof, wherein said motor endplate receptor is an acetylcholine receptor (AChR), a muscle-specific tyrosine kinase (MuSK) receptor, and / or a glutamate receptor, and / or fragments thereof, and / or a neurotoxin, wherein said neurotoxin is botulinum toxin (BoNT) and / or tetanus toxin.

The invention discloses a preparation method of a proteoglycan protein conjugate carrying a specified connection site. The method comprises the following steps: carrying out recombinant expression on a recombinant tetanus toxin heavy chain C fragment rTTHc containing a Sortase A 7M ligase recognition sequence, introducing O, O '-(ethane-1, 2-diyl) bis (hydroxylamine) as a nucleophilic reagent into the C tail end of the rTTHc under the action of ligase Sortase A 7M, and carrying out specific condensation reaction on the C tail end of the rTTHc as a unique chemical reaction site and polysaccharide carrying an aldehyde group functional group to obtain the tetanus toxin heavy chain C fragment rTTHc. Therefore, polysaccharide protein conjugates with definite polysaccharide and protein connection sites are produced. According to the invention, the problem that the key antigen epitope of the carrier protein and the heterogeneity of the product are possibly damaged by the traditional random modification strategy is solved, the antigen epitope of the carrier protein is prevented from being damaged to a great extent, and a reliable technical scheme is provided for preparing the polysaccharide conjugate vaccine with high quality and high immunogenicity.

The invention relates to the technical field of biological pharmacy, and discloses a tetanus human immune globulin preparation method, which comprises: S1, plasma pretreatment: taking anticoagulant plasma with a tetanus antibody titer of more than or equal to 10 IU / mL, and carrying out filtration treatment on the anticoagulant plasma; an improved self-adaptive algorithm is adopted to determine various parameters in the extraction process, S3, composite chromatographic purification, S4, collaborative virus inactivation, and S5, preparation preparation. According to the method, through linkage of an improved adaptive particle swarm-response surface coupling algorithm, first, 40 groups of initial sample sets are constructed by Latin hypercube sampling, a double-response surface model (R2gt; 0.95) is fitted by using a quadratic polynomial, and then a particle swarm depends on the model to predict fitness and dynamically adjust inertia weight balance optimization. By means of the linkage, the TT-IgG extraction rate is increased by 13-15%, the impurity protein removal rate is increased by 14-16%, RSD of three parallel experiments is smaller than or equal to 3%, and it is guaranteed that parameters are accurate and repeated.

The invention provides an anti-tetanus antibody as well as preparation and application thereof, and relates to the field of antibodies. The amino acid sequence of the tetanus-resistant antibody heavy chain CDR is as shown in SEQ ID NO: 1-3; the amino acid sequence of the light chain CDR of the antibody is as shown in SEQ ID NO: 4-6. The antibody disclosed by the invention is high in activity and affinity, and has the application prospect of preparing a medicine for preventing and / or treating tetanus or a product for detecting the tetanus toxin level with a non-diagnostic purpose and the like.

Provided herein are antigenic peptides comprising the SARS-COV-2 spike protein receptor binding domain (CRBD) polypeptide or portions thereof, linked to a non-catalytic, non-toxic tetanus toxin variant (i.e., a modified tetanus toxin or “MTT”) and vaccine compositions comprising the same. In addition, provided herein are methods for making and using CRBD-MTT fusion proteins as immunogenic agents.

The present disclosure provides an anti-tetanus toxin neutralizing antibody as a new generation of tetanus passive immunization preparation. The antibody can be fully human, which can specifically bind to full-length tetanus toxin and its AB fragment, has high affinity, high specificity, small toxic side effects, avoids the problems of large adverse reactions and short half-life of animal-derived monoclonal antibodies, and is prepared in large quantities by mammalian cells, and has clear and stable components.

The invention discloses a technology for removing viruses from tetanus human immune globulin through a nano film. The process comprises the following steps: pretreating a feed liquid, so that the protein concentration of the feed liquid is 50-60g / L, the pH is 3.8-4.4, and the feed liquid contains 90-110g / L maltose and 50-150mmol / L sodium chloride; then, the feed liquid is subjected to two-stage filtration through a 0.1-micron nylon pre-filtration membrane bag with negative charges and a 20-nm regenerated cellulose (RC) virus removal membrane bag in sequence. According to the process, through optimizing the physical and chemical state of the feed liquid and the synergistic effect of the feed liquid and the functionalized filter membrane, the protein aggregation is effectively inhibited, the membrane pollution is reduced, the virus removal effect is ensured not to be lower than 4log10, the protein load of the RC membrane is remarkably improved to be more than 200L / m, and the product purity is more than or equal to 98%. The invention solves the technical problems of low flux and easy blockage of high-concentration immune globulin in small-aperture virus removal filtration, and is suitable for efficient, safe and large-scale production of blood products.

The application discloses a skin test positive measurer, which comprises a storage box, a plurality of storage grooves are arranged in the storage box, a fixed shaft is fixedly installed in the storage box, a through hole is formed in the fixed shaft, a measurer main body is arranged in the storage groove, a connecting arm is fixedly connected to the outer side of the measurer main body, a connecting hole is formed in one end of the connecting arm, and a fixed shell is fixedly installed on the outer side of the storage box. Different measurer main bodies are integrated in the storage box, and the measurer main bodies are compatible with common skin test items such as penicillin, PPD and tetanus antitoxin. In combination with a multi-gradient color comparison ring and a precise scale design, the skin test positive measurer can meet the interpretation requirements of different skin test types, is suitable for clinical application scenarios of different populations such as adults and children, and has high universality. Meanwhile, the fixed shell, the movable groove, the spring, the limiting bolt, the driving block and the limiting hole are used in cooperation, so that the measurer main body in the storage groove can be fixed and limited during use, the measurer main body is prevented from easily sliding out of the storage groove, and the skin test positive measurer is convenient to carry and use.

Disclosed in the present invention is a formulation containing an anti-tetanus toxin antibody, wherein the formulation comprises the anti-tetanus toxin antibody at a concentration of 1-100 mg / mL, and has a pH of 5.0-7.0; and optionally, the formulation further comprises one or more of a surfactant, a pH regulator, a protein protective agent, and an osmotic pressure regulator. The formulation provided by the present invention can effectively maintain the stability of the antibody, so that the antibody is not liable to aggregate, thereby preventing oxidation, denaturation and degradation. In addition, the formulation also maintains the high titer of the antibody against tetanus toxin, and thus has the high biological activity required for preventing or treating tetanus.

Provided are a bispecific antibody and a monoclonal antibody for a tetanus toxin, and uses of the antibodies. The bispecific antibody contains a first antigen-binding fragment and a second antigen-binding fragment which bind to different epitopes of the tetanus toxin, and has activity of neutralizing the tetanus toxin. The monoclonal antibody binds to the tetanus toxin, and has activity of neutralizing the tetanus toxin.

C-terminal domain of the heavy chain of tetanus toxin (Hc-TeTx) provides therapeutic effects in motor impairments associated with Parkinson disease (PD), and provides long lasting antidepressant effects, thus useful in treating and mitigating depression, particularly PD-depression co-morbid condition. A method for treating or mitigating depression, including administrating an effective amount of C-terminal domain of the heavy chain of tetanus toxin (Hc-TeTx) to a subject in need thereof. A method for treating or mitigating motor impairments associated with Parkinson's disease (PD), including administrating an effective amount of C-terminal domain of the heavy chain of tetanus toxin (Hc-TeTx) to a subject in need thereof.

The utility model belongs to the technical field of separation and extraction equipment, and particularly relates to tetanus human immune globulin separation and extraction equipment which comprises a protective shell, a rotating disc is rotatably mounted in the protective shell, a plurality of placement cavities and a first power cavity are formed in the rotating disc, and clamping rods are slidably mounted in the first power cavity and located on the two sides of the placement cavities; one end of the clamping rod penetrates through the power cavity I and extends into the corresponding placing cavity; the driving assembly is located in the protective shell and used for driving the rotating disc to rotate and driving the multiple clamping rods to slide; the fixed block is fixedly mounted on one side of the protective shell, a sliding groove is formed in the fixed block, and a storage tank is slidably mounted in the sliding groove, so that the positions of the plurality of test tubes can be clamped and fixed, the stability of the test tubes during rotation is ensured, and meanwhile, to-be-extracted products in the plurality of test tubes are ensured to be the same; the same time and rotating speed required by separation and extraction of a plurality of test tubes are ensured, and the same quality of separated and extracted products is ensured.

This utility model relates to the technical field of human immunoglobulin preparation devices, and discloses a sampling device for preparing tetanus human immunoglobulin. It includes a sampling cylinder with a low-temperature storage mechanism on its outer wall and an automatic sampling mechanism. The low-temperature storage mechanism includes a first support ring, a supporting outer cylinder fixedly mounted at the bottom of the first support ring, and a second support ring fixedly mounted at the bottom of the supporting outer cylinder. Through the design of the low-temperature storage mechanism, this utility model can store a low-temperature medium in the cavity formed by the first support ring, the supporting outer cylinder, the second support ring, and the copper inner cylinder. The low-temperature medium can pass through the copper inner cylinder to cool the inner cavity of the sampling cylinder, thus achieving the function of low-temperature storage of the sample inside the sampling cylinder. This avoids the problem of sample deterioration during storage due to high ambient temperatures, ensuring the accuracy of biochemical analysis.

Disclosed in the present invention is a compound adjuvant combined vaccine, comprising an immunogenic composition and a compound adjuvant. The immunogenic composition comprises a pertussis antigen, a diphtheria antigen and a tetanus antigen; and the compound adjuvant is composed of an aluminum adjuvant and a TLR9 receptor agonist. Further disclosed in the present invention is a use of the compound adjuvant in the preparation of the compound adjuvant combined vaccine for preventing pertussis, diphtheria and tetanus in a subject.