222 results about "Tetanus" patented technology

Methods of using tetanus toxin to modulate or control neural functions or nonneural cellular activities at selected sites in animals, particularly in mammals, and more particularly in humans, are provided. Pharmaceutical formulations to modulate neural functions or non-neural cellular activities of an animal at selected sites in animals, particularly in mammals, and more particularly in humans are also provided. Uses of tetanus toxin in preparation of medicaments for methods of treating clinical disorders or symptoms of animals, particularly mammals and more particularly humans are also provided.

The disclosure relates to cancer ribonucleic acid (RNA) vaccines, as well as methods of using the vaccines and compositions comprising the vaccines. In particular, the disclosure relates to concatemeric mRNA cancer vaccines encoding several cancer epitopes on a single mRNA construct, i.e. poly-epitope mRNA constructs or poly-neo-epitope constructs. The disclosure further relates to p53 and KRAS mutations, as well as incorporation of immune enhancers such as STING, e.g. mRNA constructs further encoding an immune stimulator or adjuvant. The disclosure further relates to inclusion of universal T cell epitopes, such as tetanus or diphtheria toxins to elicit an enhanced immune response.

Disclosed are vaccines and vaccine adjuvants useful in the treatment and / or prevention of infection and diseases associated with infectious pathogens, such as tetanus, as well as diseases associated with biological toxins. Also provided are methods of preparing an adjuvant and the vaccine containing the adjuvant. Methods are also provided for vaccinating / immunizing an animal against infection and diseases associated with infectious pathogens, such as tetanus, and other diseases associated with biological toxins. Adjuvant materials are presented that are prepared from an extracellular matrix material. The adjuvants are demonstrated to enhance the immunogencity of an infectious pathogen antigen or biological toxin antigen of interest, as well as to enhance the survival of an immunized animal.

A hybrid protein contains a protein that binds to a receptor of mastocytes and basophils and is endocyted by them. The protein can be IgE; IgE fragment; IgE Fc fragment; antibody against IgE receptor of mastocytes and basophils; fragment of the antibody against the IgE receptor of mastocytes and basophils; antibody against mastocyte specific potassium channel; and mast cell degranulating peptide. The hybrid protein also contains a protease cleaving proteins of the secretion process of the mastocytes and basophils so as to inhibit the secretion process without killing the mastocytes and basophils. The protease can be light chain Clostridium botulinum toxin; proteolytically active fragment of the light chain of a Clostridium botulinum toxin containing an amino acid sequence His-Xaa-Xaa-Xaa-His-Xaa-Xaa-His wherein Xaa is an amino acid; light chain of the tetanus toxin; proteolytically active fragment of the light chain of the tetanus toxin containing His-Asp-Leu-lIe-His-Val-Leu-His; IgA protease of Neisseria gonorrhoeae; and proteolytic domain of the IgA protease of Neisseria gonorrhoeae.

The invention relates to human rotavirus Delta VP8* subunit recombinant protein and application thereof. The human rotavirus Delta VP8* subunit recombinant protein comprises a T cell epitope P2 in tetanus toxin and a rotavirus Delta VP8* subunit. By the recombinant protein disclosed by the invention, the immune efficacy of a Delta VP8* subunit vaccine can be greatly improved; faster and stronger neutralization antibody titer can be induced; moreover, anti-p[4] genotype specific rotavirus cross neutralization antibody of high titer can be induced; simultaneously, the potential risk of inducing intussusception by taking attenuated rotavirus vaccine orally can be overcome; therefore, the recombinant protein is applicable to preparing a rotavirus vaccine.

The invention relates to a Chinese medicine preparation for curing tetanus. The formula includes earthworm, cicada ecdysis, rhizoma gastrodiae, notopterygium root, parsnip, herba schizonepetae, rhizoma arisaematis, uncaria, red peony, alum, ophiopogon japonicus, cortex moutan, Indian bread with hostwood, root poria, centipede, whole worm, atrina glass, honeysuckle, hemlock parsley, forsythia, safflower, peach seed and angelica. In the formula, the raw materials are pulverized, filtered and then made into pulvis. During the medicine taking, the medicine is mixed with cold boiled water, with two or three times per day and three days is one treatment period. The Chinese medicine preparation, compared with the prior art, is scientific and reasonable in the formula, simple in the regrouping ofthe raw materials and preparation process, effective in reducing the treatment cost and remarkable in treating tetanus. Having no toxic side effects to the human body, the invention is the medicine prepared commonly in homes.

The invention relates to a method for producing health care black bone chicken egg and employed pharmaceutical composition. The production method of the health care black bone chicken egg comprises the following steps: (1) respectively grinding and then evenly mixing medicinal materials in the main drug; (2) respectively grinding and then evenly mixing medicinal materials in the adjuvant; (3) fully mixing the main drug and the adjuvant obtain in step (1) and step (2) to obtain the pharmaceutical composition, wherein the proportioning is weight of main drug: weight of adjuvant=80-150:100; (4) adding the pharmaceutical composition to the feed to be evenly mixed to form a mixed feed; wherein the additive amount is that weight of the pharmaceutical composition: weight of the feed=5-20:100; (5) feeding the mixed feed obtained in step (4) to egg-producing black bone chicken, then obtaining the health care black bone chicken egg which is produced after feeding the black bone chicken for 7 days.The pharmaceutical composition is rationally matched by selecting multiple medicines, through efficient biotransformation of the egg-producing black bone chicken, cream of Chinese herbal medicines are concentrated in the eggs, so that the health care black bone chicken eggs with multiple health care and treatment functions are obtained; the health care black bone chicken eggs enjoy excellent food therapy effect in preventing and treating diabetes, high blood pressure, hemiplegia, sequel of apoplexy, cardiovascular and cerebrovascular diseases, rheumatism and paralysis, tetanus, infertility and various cancers.

The present invention provides clostridial toxin substrates useful in assaying for the protease activity of any clostridial toxin, including botulinum toxins of all serotypes as well as tetanus toxins. A clostridial toxin substrate of the invention contains a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence that includes a cleavage site, where the cleavage site intervenes between the donor fluorophore and the acceptor and where, under the appropriate conditions, resonance energy transfer is exhibited between the donor fluorophore and the acceptor.

The invention discloses an anti-tetanotoxin antibody. In the heavy chain variable region, the amino acid sequence of CDR1 is disclosed as SEQ ID NO.5, the amino acid sequence of CDR2 is disclosed as SEQ ID NO.6, and the amino acid sequence of CDR3 is disclosed as SEQ ID NO.7; and in the light chain variable region, the amino acid sequence of CDR1 is disclosed as SEQ ID NO.8, the amino acid sequence of CDR2 is disclosed as SEQ ID NO.9, and the amino acid sequence of CDR3 is disclosed as SEQ ID NO.10. The invention also discloses a nucleotide sequence for coding the anti-tetanotoxin antibody, and a corresponding recombinant plasmid and recombinant expression vector. The invention also discloses a preparation method and application of the anti-tetanotoxin antibody. The anti-tetanotoxin antibody disclosed by the invention is a full human antibody, has the advantages of low side reaction, high affinity and simple preparation method, and has wide industrial application prospects.

The invention discloses a preparation method of an ultramicro gastrodia elata decoction piece. The preparation method comprises selection of raw materials, cleaning of the raw materials, boiling, slicing, secondary boiling, drying, ultramicro wall breaking, obtaining of a preparation, and the like. Gastrodia elata processed by employing the processing and ultramicro fragmentation method has relatively high effective-composition dissolution rate, the bioavailability of a medicine is improved, the medicine effect is relatively good, and the toxicity is relatively low. The invention also discloses the decoction piece prepared by employing the method, and application of the decoction piece to prepare medicines for treating headache dizziness, numbness of limbs, infantile convulsion, epilepsy, twitch, tetanus and other diseases.

The present invention relates to a combination vaccine comprising a mixture of antigens for protection against diseases such as diphtheria, tetanus, acellular pertussis, and infections caused by Haemophilus influenzae and polio viruses. The present invention also relates to inclusion of antigens for protection against infections caused Hepatitis virus and other pathogens, such that administration of the vaccine can simultaneously immunize a subject against more than one pathogen. The invention in particular relates to a fully liquid stable combination vaccine comprising the antigens as indicated above and the methods for manufacturing the same.

The invention relates to a nucleic acid construct for delivery into living cells in vivo for inducing an immune response in a patient to an idiotypic determinant present on a malignant B cell in the patient; the construct directing the expression of a fusion protein, said fusion protein comprising the idiotypic determinant and at least one T helper cell epitope from tetanus toxin. The invention further relates to a method of making the nucleic acid construct, a method of treating a patient, and to a composition comprising the nucleic acid construct.

The invention discloses a traditional Chinese medicine preparation for treating tetanus, which comprises the following raw material medicaments in parts by weight: 15 parts of taraxacum, 15 parts of fish flabellate, 15 parts of exuviae cicada, 15 parts of scorpio, 8 parts of white silkworm, 8 parts of gastrodia, 8 parts of rhizoma arisaematis, 8 parts of hurricane lamp, 8 parts of angelica, 8 parts of herba schizonepetae, 8 parts of notopterygium root and 8 parts of annulus enclosure. The preparation comprises a pill, a powdery preparation, a tablet, an oral liquid preparation and a capsule prepared by a traditional preparation technology or a modern preparation technology. The adopted medical materials have the efficacy of clearing away heat and toxic material, removing carbuncle and eliminating stagnation, dispersing stagnated liver for regulating qi, promoting blood circulation for removing blood stasis, and relieving exterior syndrome for dispersing cold. The traditional Chinese medicine preparation enhances the immune function of an organism by oral administration, has good treating effect on the tetanus caused by various diseases, and is convenient to take. Clinical application proves that the traditional Chinese medicine preparation has the advantages of quick action, obvious treating effect, good durability, low cost and no any toxic or side effect.

The invention belongs to the technical field of lateral side stream chromatography and bioanalysis, particularly relates to a test strip for detecting tetanus antibody on basis of quantum dot fluorescent microsphere chromatography and a preparation method and using method thereof, and aims at solving the technical problems in the process of rapidly and portably detecting tetanus antibody content via low consumption. The preparation method of the test strip comprises the following steps of: firstly closing a sample pad by using solution with a TW20 mass concentration of 0.5-5%; marking a detection line and a quality control line on an NC membrane in sequence; drying the processed sample pad and the NC membrane at 37 DEG C for 1 hour; pasting the sample pad, the NC membrane and a water absorption pad on a PVC baseboard in sequence; carrying out overlapping in pairs to 2-3 mm; and finally cutting into 3.9 mm wide to obtain the finished test strip. Compared with traditional colloidal goldimmunochromatography test strips, the test strip is good in quantum dot microsphere marker stability and high in detection sensitivity, and is capable of realizing quantitative detection for low-concentration target objects.

A method for visualizing an active synapse wherein said method comprises: (a) exposing cells forming the active synapse to a biomarker comprising at least fragment C of tetanus toxin and a reporter protein; and (b) visualizing the biomarker; wherein the accumulation of the biomarker into dendritic spines of the cells allows visualization of an active synapse. Also, a method for screening molecules capable of modulating synapse activity is provided. A kit useful for the early diagnosis of neurodegenerative disease comprises a biomarker comprising at least fragment C of tetanus toxin and a reporter protein.

The present invention relates to the field of vaccines for protecting against polio, and in particular to combination vaccines for protecting against polio, diphtheria, tetanus, and pertussis diseases. Specifically, vaccines comprising reduced dose inactivated poliovirus are provided

The invention provides a diphtheria, tetanus and acellular pertussis / Haemophilus influenzae type b - group A and C meningococcus combined vaccine, which has the characteristics of high safety, high efficacy, high controllability and prevention of multiple diseases through one injection, satisfies relevant requirements on absorbed diphtheria, tetanus and acellular pertussis combined vaccines, Haemophilus influenzae type b combined vaccines and group A and C meningococcus combined vaccines in the Third Part of Chinese Pharmacopoeia (2010) and proposed Rules on Production and Inspection of Diphtheria, Tetanus and acellular Pertussis / Haemophilus influenzae Type b - Group A and C Meningococcus Combined Vaccines, and achieves an effect of putting into practical application.

Provided herein are combination vaccines comprising antigens for protecting a subject against at least diphtheria, tetanus, pertussis and Hib, wherein: (a) the antigen for protecting against Hib is a conjugate of a Hib capsular saccharide; (b) the concentration of the Hib conjugate in the vaccine is <15 μg / ml; and (c) the Hib conjugate has never been lyophilised.

The invention relates to the filed of pharmaceutical preparation, in particular to single dose immunization against tetanus toxin cation dextran microspheres and a preparation method thereof. The single dose immunization against tetanus toxin cation dextran microspheres are prepared by carrying tetanus toxin after electrostatic interaction on cation hydroxyethyl acrylate dextran microspheres. The tetanus toxin controlled release microspheres can reduce the injection frequency of tetanus vaccine, improve the vaccination coverage and reduce the drop-out rate, thereby effectively preventing tetanus and providing a single dose tetanus toxin controlled release vaccine preparation with long-term effect and realizing the whole course immunity by one injection.

The invention discloses a clostridial neurotoxin substrate protein, and a coding gene and an application thereof. The clostridial neurotoxin substrate protein is a protein (a) composed of an amino acid sequence represented by sequence 2 in a sequence table, or is a protein (b) obtained through substituting and / or deleting and / or adding one or more amino acid residues to the amino acid sequence represented by the sequence 2, related to the clostridial neurotoxin detection and derived from the sequence 2. The substrate protein SNVP provided by the invention has seven toxin serotype botulinum and tetanus toxin enzyme hydrolysis sites, can specifically identify A, B, E, C, D, F and G type botulinum toxins and tetanus toxins, and can be used for the detection and the parting discrimination of the botulinum toxins and tetanus toxins as a core detection reagent.

The invention relates to a tetanus treatment traditional Chinese medicine preparation, which is prepared from the following bulk drugs, by weight, 13-17 parts of Chinese bastardtoadflax herb, 13-17 parts of fragrant solomonseal rhizome, 13-17 parts of paniculate bolbostemma bulb, 13-17 parts of white peony root, 10-14 parts of milkvetch root, 10-14 parts of unprocessed rehmannia root, 10-14 parts of asiatic plantain herb, 9-11 parts of angelica, 9-11 parts of centipede, 9-11 parts of stiff silkworm, 6-8 parts of sharpleaf uncaria stem with hooks, 6-8 parts of giant typhonium tuber, 6-8 parts of divaricate saposhnikovia, 4-6 parts of scorpion, and 4-6 parts of reddish jackinthepulpit tuber. The tetanus treatment traditional Chinese medicine preparation has advantages of significant treatment effect, rapid effect and low toxic-side effect, wherein clinical trial results show that tetanus treatment efficiency is 96%.

The invention discloses a neutralizing monoclonal antibody resisting to tetanus toxin. Hybridoma cells are obtained from tetanus toxin heavy chain C fragment immune mice, light chain and heavy chain variable region genes of the antibody are taken, human-mouse chimeric complete antibody expression vectors are built, CHO cell transfection is carried out, purification is carried out, and then the antibody is obtained. The antibody has the activity of specific binding to tetanus toxin, the monoclonal antibody can partially protect mice against attack of tetanus toxin, and four antibodies can completely protect mice against a twofold lethal dose of tetanus toxin in combination.

The ointment for healing traumatic injury fast is prepared with 21 kinds of Chinese medicinal materials including ledebouriella root, dahurian angelica root, ground beetle, dragon's blood, cimicifugarhizome, etc. The preparation process includes soaking, drying, grinding into fine powder, sieving, mixing, bottling and other steps. When used, it is mixed with proper amount of turpentine, Vaselineor spirit to produce ointment. It has the integrated functions of stopping pain, stopping bleeding, relaxing muscles and joints, dispersing bloood clots, elimianting swelling, expelling wind and wetness, promoting healing of fracture and preventing tetanus and has no toxic side effect.

The invention discloses a hydrophobia-tetanus double titer human immunoglobulin, wherein the purity of the immunoglobulin is more than or equal to 90 percent of total protein, the titer of the hydrophobia neutralizing antibody is more than or equal to 100IU / mL, the titer of the tetanus neutralizing antibody is more than or equal to 40IU / mL, and the content of protein is less than or equal to 180g / L. In addition, the invention also discloses a method for preparing the hydrophobia-tetanus double titer human immunoglobulin and application of the hydrophobia-tetanus double titer human immunoglobulin in preparing medicaments for preventing hydrophobia and tetanus.

Disclosed are vaccines and vaccine adjuvants useful in the treatment and / or prevention of infection and diseases associated with infectious pathogens, such as tetanus, as well as diseases associated with biological toxins. Also provided are methods of preparing an adjuvant and the vaccine containing the adjuvant. Methods are also provided for vaccinating / immunizing an animal against infection and diseases associated with infectious pathogens, such as tetanus, and other diseases associated with biological toxins. Adjuvant materials are presented that are prepared from an extracellular matrix material. The adjuvants are demonstrated to enhance the immunogenicity of an infectious pathogen antigen or biological toxin antigen of interest, as well as to enhance the survival of an immunized animal.

Serogroup B meningococcus antigens can successfully be combined with diphtheria, tetanus and pertussis toxoids (“DTP”) to provide effective combination vaccines for protecting against multiple pathogens. These combinations are effective with a range of different adjuvants, and with both pediatric-type and booster-type DTP ratios. The adjuvant can improve the immune response which the composition elicits; alternatively, by including an adjuvant it is possible for the compositions to have a relatively lower amount of antigen while nevertheless having immunogenicity which is comparable to unadjuvanted combination vaccines.

The invention discloses a medical hemostasis first-aid belt and a hemostyptic thereof. The first-aid belt comprises a body, wherein the periphery of the bottom surface of the body is provided with a gluing region at which an oily leather is arranged; the middle part of the body is provided with a hemostasis bag; and vacuum air bags are arranged at the periphery of the hemostasis bag. In addition, the invention further discloses the hemostyptic used for the medical hemostasis first-aid belt. The hemostyptic comprises medicaments with hemostasis, diminish inflammation, analgesia, infection prevent and tetanus prevention effects, such as common cephalanoplos herb, pseudo-ginseng, cicada slough, rhizoma arisaematis, corydalis tuber, bezoar, borneol, pearl powder, red paeonia, excrementum pteropi and dalbergia wood. Since the technical scheme is adopted, compared with the prior art, the medical hemostasis first-aid belt disclosed by the invention has the characteristics of simple structure, convenience in use, portability, good hemostasis effect, less possibility of infection, wide application range and the like.

The invention discloses a completely humanized monoclonal neutralizing antibody for a tetanus toxin. The completely humanized anti-tetanus toxin neutralizing antibody is developed via a comprehensivehigh-throughput completely humanized monoclonal antibody research and development technical platform. The neutralizing antibody is high in affinity and specificity and has an important significance onprevention and treatment of tetanus infection.

The invention discloses a Chinese herba preparation for treating tetanus. The selected crude drugs comprise the following components by weight: 20-30g of nam sing, 10-20g of gastrodia elata, 10-20g of periostracum cicada, 10-20g of bezoar, 10-20g of notopterygium incisium, 10-20g of pawpaw, 10-20g of cassia twig, 10-20g of tribulusterrestris, 10-20g of centipede, 8-15g of fructus evodiae, 8-12g of antelope horn, 8-12g of tabasheer, 8-12g of dandelion, 8-12g of coptis chinensis and 8-12g of astragalus membranaceus. The medicines are of good compatibility, plays the functions of supporting right and banking up the root, tranquilizing the mind, carrying out spasmolysis, activating blood, promoting the circulation of qi, perfusing meridian, relieving cough and eliminating phlegm. Clinical application finds that the Chinese herba preparation has the advantages of rapid effect taking, short treatment course, no side effect and high cure rate for treating tetanus, and is worthy of clinic promotion and application.

The invention discloses a Chinese herbal medicine for treating tetanus, which relates to a medicament preparation product and aims to increase a medicine variety for treating the tetanus. The Chinese herbal medicine comprises the following raw materials: 0.5-4g of climbing groundsel herb, 2-25g of Alangium platanifolium, 1-10g of serissa and 0.5-5g of wild chrysanthemum flower. The preparation method comprises the following steps of: cleaning the climbing groundsel herb, the Alangium platanifolium, the serissa and the wild chrysanthemum flower, mincing the raw materials and drying, crushing and milling, evenly mixing according to mass composition of each pharmaceutical formulation, and sealing and packaging and storing in a cool and dry place to obtain the medicine for later use. The usage method comprises the steps of boiling the medicine by adding with water, fumigating infected parts through steam, and washing the infected parts by warm medicinal water. The Chinese herbal medicine has the functions of clearing away heat and toxic materials, inflammation diminishing and swelling reducing, detoxifying, and being capable of restraining and killing clostridium tetani vitality. The raw materials are easily obtained, the cost is low, the preparation process is simple, and the medicine is suitable for patients of the tetanus.