1407 results about "Test strips" patented technology

The invention belongs to the application of a computer technology in the field of shot blasting processing, and discloses a shot blasting strength calculation method and system based on control parameters of intelligent shot blasting equipment. According to the method, an intelligent calculation method system is constructed by establishing a prediction model of the control parameters and the shot blasting strength. According to the method, nozzle pressure, moving speed, spraying angle, shot flow and target distance parameters of shot blasting equipment are collected in real time, workpiece material attribute data are combined, a multi-dimensional data fitting algorithm is adopted for processing, and an accurate shot blasting strength value is output. Meanwhile, a self-adaptive parameter adjusting module is developed, and dynamic calculation optimization can be carried out according to residual stress field distribution and coverage rate requirements. Compared with a traditional Almen test piece method, the method achieves real-time calculation of shot blasting strength and closed-loop control of technological parameters, the calculation error rate is reduced to be within 5%, test piece consumption is reduced by 80% or above, machining efficiency and quality stability are remarkably improved, and an innovative solution is provided for intelligent manufacturing in the field of surface strengthening.

The invention provides a test strip for rapidly detecting methicillin-resistant staphylococcus aureus and application of the test strip, and belongs to the technical field of biochemical detection. The test strip comprises a bottom plate, a sample pad, a combination pad, a detection pad and a water absorption pad, the two ends of the detection pad are in lap joint with the combination pad and the water absorption pad respectively, the detection pad is located on the lower portion, the sample pad is in lap joint with the combination pad, and the combination pad is located on the lower portion; a detection line and a quality control line are arranged on the detection pad, the detection line is arranged on one side close to the combination pad, and the quality control line is arranged on one side close to the water absorption pad; the detection pad is coated with a MoS2 nanosheet, the combination pad is coated with a penicillin binding protein 2a polyclonal antibody labeled by horse radish peroxidase, and the detection line is coated with a penicillin binding protein 2a monoclonal antibody; the quality control line is coated with goat anti-rabbit IgG (immunoglobulin G). The kit is high in sensitivity and wide in linear range, has good specificity, repeatability and stability, and can effectively detect MRSA in a clinical sample.

A specimen collection, storage, transport, and testing device can include a vessel carrying a liquid reagent. A lid sealing the vessel can include a solid specimen collection stick projecting from an inside surface of the lid. Further, the lid can include a port on its outside surface. The port can be releasably engaged by a hand-manipulable handle facilitating specimen collection and attachment of the lid to the vessel. The port can further include an openably sealed aperture for accommodating the insertion of a test strip-carrying cartridge. The reusability of the handle and its reduced material structure helps avoid waste.

The invention discloses a micro-plastic integrated intelligent detection device, which comprises: a sample pretreatment module, which comprises a sample mixing module, a filtering separation module, an enrichment concentration module and a surface modification and marking module which are communicated in sequence and is used for mixing, filtering, enriching, modifying and marking a sample in sequence; the immunoreaction module is used for performing immunoreaction on the sample and the anti-polystyrene monoclonal antibody freeze-dried product marked by the quantum dot fluorescent microspheres; the fluorescence detection module is used for carrying out fluorescence detection on the test strip subjected to the immune reaction; the automatic control module is electrically connected with the sample pretreatment module, the immunoreaction module and the fluorescence detection module and is used for controlling the operation of each module. According to the invention, the sample processing module, the reaction module, the detection module and the control module are in close cooperation, the sample enters each module in sequence after simple pretreatment, detection and result output are semi-automatically completed, frequent manual operation of different instruments is not needed, the operation steps and time are reduced, and the operation difficulty is reduced.

The invention relates to the technical field of bacterium detection, in particular to a colorimetric-Raman coding immunochromatography test strip based on a 3D film-shaped SERS probe and a multi-bacterium detection method thereof. According to the technical scheme, the kit comprises a sample pad, a nitrocellulose membrane, a water absorption pad, a 3D membrane-like SERS probe and an operation buffer solution. According to the present invention, the immunochromatography of the 3D film-like probe is provided, the MoDAu-coated Ag SERS nano-film is adopted as the probe in the system, such that the pathogenic bacteria can be highly specifically recognized and captured, the immune complex fluidity can be increased, the rapid quantitative detection of the three pathogenic bacteria can be achieved, and the flexible 3D film-like SERS probe capable of enhancing the colorimetric ability and the SERS activity and highly specifically capturing the pathogenic bacteria can be prepared; by combining with an antibody-modified ICA test strip, rapid, simultaneous and quantitative determination of various pathogenic bacteria is realized.

The invention relates to a centrifugal micro-fluidic chip for automatic detection of a nano-enzyme test strip and application of the centrifugal micro-fluidic chip. The centrifugal micro-fluidic chip integrates the steps of sample adding, chromatography, color development and the like of the nano-enzyme test strip, and full automation of nano-enzyme experiments on the micro-fluidic chip is realized. Comprising a reaction structure main body, a bottom runner layer, a vertical through hole layer, a pressing layer, a top runner layer and a sealing body. The closed structure of the chip effectively reduces the interference of external environmental factors on the experiment, reduces the risk of sample pollution, and ensures the high reliability of the detection result.

The invention relates to the technical field of biological detection, and particularly discloses a test strip and a kit for detecting Alzheimer-related neurofilament protein AD7c-NTP. The detection test strip for the Alzheimer-related neurofilament protein AD7c-NTP provided by the invention comprises a PVC (Polyvinyl Chloride) bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad are arranged on the PVC bottom plate; a mouse anti-AD7c-NTP monoclonal antibody I marked by latex is contained on the combination pad; a detection line T and a quality control line C are arranged on the nitrocellulose membrane; the detection line T is coated with a mouse anti-AD7c-NTP monoclonal antibody II, and the quality control line C is coated with a goat anti-mouse IgG polyclonal antibody. The detection test strip for Alzheimer-related neurofilament protein AD7c-NTP provided by the invention has the advantages of high sensitivity, strong specificity, good stability and the like, can realize auxiliary diagnosis of Alzheimer's disease, and has a wide application prospect.

A station for a urine analysis device is proposed, the station including a case, intended to be positioned at least partially within the toilet, a housing, within the case, configured to at least partially receive a cartridge comprising a plurality of test supports (e.g. test strips). The cartridge is movable within the housing. The station may also include an optical sensor, a translatory injector and an analyzer (e.g. optical). Various configurations are available.

The invention belongs to the technical field of microbiological detection, and particularly relates to a closed nucleic acid extraction, amplification and detection fully-integrated device. Comprising an injector type nucleic acid extraction, enrichment and amplification assembly, a detection card box, a three-way valve and a sealing cover, wherein the nucleic acid extraction, enrichment and amplification assembly comprises a chitosan modified paper sheet used for nucleic acid enrichment and an injector, the chitosan modified paper sheet is fixed in a needle cylinder of the injector through a rubber plug, and a piston of the injector is isolated from the chitosan modified paper sheet through isolation liquid; the detection card box is internally provided with at least one test strip, a box body is provided with a vent hole, the vent hole is covered with a film, and the film is breathable and waterproof; when an amplification product is loaded, one end of the three-way valve is connected with the detection card box, the other end of the three-way valve is connected with the nucleic acid extraction, enrichment and amplification assembly, and the third end of the three-way valve serves as a liquid inlet; during detection, the sealing cover is connected with the third end of the three-way valve.

The invention relates to a pesticide residue detection test strip as well as a preparation method and application thereof, in particular to a rapid detection technology based on an enzyme inhibition method and a chitosan non-woven fabric carrier. The test strip can specifically detect organophosphorus and carbamate pesticides such as trichlorfon, malathion and phoxim through the enzyme activity inhibition principle, the detection limit of the pesticides such as phoxim is as low as 0.1 mg / kg, the developing intensity is in negative correlation with the pesticide concentration, and semi-quantitative judgment can be achieved. The preparation process is simple, the chitosan non-woven fabric is adopted as a fixed carrier, the material is environment-friendly and degradable, the enzyme activity recovery rate reaches 90%, the stability is excellent (the enzyme activity recovery rate can be preserved for 60 days at the temperature of 4 DEG C in a dark place), sample pretreatment is not needed in detection operation, a result can be obtained at the normal temperature for 15 min, the stability and repeatability are good, and the problems that a traditional instrument is high in detection cost and long in time consumption are solved; the method has a remarkable basic popularization value.

Disclosed is a multiplex quantum dot-based quantitative test card, and a preparation method, apparatus, and use method thereof in the technical field of biological testing, the multiplex quantum dot-based quantitative test card including a multiplex card housing and a test strip, where the multiplex card housing is provided with a plurality of reaction slots configured to accommodate the test strip; the multiplex card housing further includes a plurality of sample loading ports and a plurality of observation windows, where the sample loading ports and the observation windows are in one-to-one correspondence with the reaction slots; and the test strip includes a conjugate pad coated with quantum dot-labeled antibodies, where the quantum dot-labeled antibodies serve as chromogenic substances.

The invention belongs to the technical field of molecular diagnosis, and particularly discloses a high-risk HPV16 rapid detection system based on RPA-CRISPR / Cas12a, which is prepared by the following steps: (1) mixing a nucleic acid lysis solution containing Tris (pH = 8.0), NaHCO3, polyvinylpyrrolidone and Tween-20 with Proteinase K to treat a sample, and performing pyrolysis at room temperature for 5 minutes to obtain a nucleic acid template; (2) an RPA reaction system contains an RNA inhibitor, a reverse transcriptase and an RPA reaction essential enzyme, and after the template and the starting agent in the step (1) are added, RPA amplification is carried out at 37 DEG C; (3) dropping the reaction liquid on a colloidal gold test strip subjected to position exchange and transformation of a sample pad and a water absorption pad, and carrying out result interpretation; and (4) adding the RPA product into a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) reaction system containing the LbCas12a protein, the specific gRNA and the probe. According to the scheme, a rapid, high-sensitivity and cross-reaction-free detection system of the HPV16 is realized by optimizing a nucleic acid lysis solution formula, designing a specific primer and gRNA and modifying a test strip for detection.

The invention discloses a test strip for detecting etoxazole and application of the test strip. The test strip comprises a sample absorption pad (1), a conjugate release pad (2), a reaction film (3), a water absorption pad (4) and a bottom plate (7), the reaction film is provided with a detection line (5) coated with an etoxazole hapten-carrier protein conjugate and a quality control line (6) coated with a goat anti-mouse antibody, and the conjugate release pad (2) is sprayed with an etoxazole monoclonal antibody-colloidal gold marker. The invention also provides a method for detecting residual etoxazole in vegetables and fruits by using the etoxazole test strip. The test strip provided by the invention has the characteristics of simplicity in operation, high sensitivity, high detection speed, low cost and the like, and is suitable for screening and on-site monitoring of a large number of samples.

The invention discloses beryllium element detection test paper as well as a preparation method, a detection method and application thereof. The beryllium element detection test paper comprises a filter paper base material, a color developing agent chrome azurol S and a shielding agent ethylene diamine tetraacetic acid disodium salt, the color developing agent chrome azurol S and the shielding agent disodium ethylene diamine tetraacetate are loaded on the filter paper base material. When the beryllium element detection test paper is used for detecting a surface beryllium pollution detection object, the detection method is simple and rapid to operate, high in sensitivity and low in cost.

The invention provides a pepsin detection card and a preparation process thereof, and belongs to the technical field of microbiological detection. The kit comprises a test strip and a card shell, the test strip comprises a nitrocellulose membrane, a sample pad, absorbent paper, a combination pad and a bottom plate. The pepsase detection card is high in sensitivity, can accurately capture pepsase with extremely low concentration in a sample, effectively avoids missed diagnosis, strives for precious time for early intervention, is high in specificity, is only specifically combined with pepsase, is not interfered by other similar proteases or impurities in the sample, and can be used for detecting the pepsase in the sample. In a complex human body biological sample environment, the target pepsin can still be accurately recognized, the specificity reaches 98% or above, false positive results are greatly reduced, and a reliable basis is provided for clinical diagnosis.

According to the preparation and application of the novel Fe monatomic nano enzyme, mesoporous silica is used as a substrate material, the surface of the substrate material is coated with a layer of dopamine and hemin, then high-temperature calcination is performed to form the monatomic nano enzyme Fe-N-C, then the monatomic nano enzyme Fe-N-C is used as a signal reporter molecule of a lateral flow chromatography test strip, and the application of the monatomic nano enzyme Fe-N-C in the lateral flow chromatography test strip is realized. A labeled antibody is connected to the surface of the antibody, so that rapid detection of cardiac troponin I (cTnI) is realized. In the monatomic nano-enzyme, dopamine mainly provides a carbon source, heme mainly provides Fe and a nitrogen source, the larger specific surface area of mesopores provides abundant sites for the monatomic nano-enzyme, and the contact area of the monatomic nano-enzyme and a substrate is increased, so that the enzyme activity is improved. In conclusion, the material has a large specific surface area and relatively high enzyme activity. And finally, quickly detecting the biomarker cTnI after the test strip platform is integrated.

The invention discloses a lateral flow chromatography test strip for detecting perfluorooctanoic acid by combining gold nanoparticles, a nucleic acid aptamer and an antibody. According to the test strip, a nucleic acid aptamer marked by gold nanoparticles is combined with PFOA molecules to form a nucleic acid aptamer with a G-quadruplex structure, and then the nucleic acid aptamer with the G-quadruplex structure is specifically recognized and captured by an anti-BG4 antibody, so that specific visual detection of perfluorooctanoic acid is realized. After a sample is injected, a G-quadruplex structure formed by the PFOA and the nucleic acid aptamer acts with the anti-BG4 antibody coated on the test strip, so that the gold nanoparticles are agglomerated to generate a chromogenic reaction, the signal intensity can be detected by naked eyes or reading equipment, the visual detection limit is 10 mu M, and the equipment detection limit is 304 nM. The test strip has the advantages of high detection speed, high specificity and high sensitivity, and is suitable for field detection and rapid screening. Compared with a traditional method, the method realizes simple, convenient and economical detection of PFOA, and has a wide application prospect.

The invention relates to methods, devices, and software for distinguishing hemoglobin (Hb) from myoglobin (Mb) in urine samples using peroxidase-reactive test strips. A time-resolved video of the color change on the test pad is recorded, and hue values are extracted and analyzed over time. Diagnostic differentiation between Hb and Mb is achieved by evaluating the hue-time curve, in particular via endpoint hue values and the ratio of integrated hue values in distinct time intervals. The invention further comprises a standardized optical setup for recording, a software-based evaluation algorithm, and a refillable cartridge for in-situ urine analysis devices containing appropriate test fields. The invention enables combined testing with additional urine parameters and is suitable for medical and non-clinical applications such as sport and home diagnostics.

A test strip for detecting uric acid in saliva that meets the requirement for room-temperature preservation, a preparation and detection method therefor and the use thereof. The test strip for detecting uric acid in saliva comprises a uricase, a peroxidase, a vitamin C oxidase, a bilirubin oxidase, a color developing agent, and a protective film coated on the test strip for detecting uric acid in saliva. To meet the needs of a target population for long-term monitoring and real-time tracking of changes in their uric acid levels, by means of agent addition, the test strip for detecting uric acid in saliva has prolonged preservation time at room temperature, enables non-invasive, efficient and convenient monitoring of a health status at any time, and timely detection and treatment, allows intuitive visual identification of uric acid levels in saliva by a subject, and meets the needs of a subject for long-term preservation at room temperature.

A coupled enzyme reaction point of care assay system detects and measures an active enzyme biomarker from a patient's blood sample. The assay system has a point of care blood collector for collecting blood into a blood collection vial. A biomarker detection mechanism has at least three fluid flow entities. Each fluid flow entity has a fluid input zone and a corresponding reaction zone. The fluid flow entities include a test strip, a negative control strip and a positive control strip. The reaction zones include components from a multiplicity of substrates, co-factors, buffers and cryoprotectants, and a multiplicity of tethered enzymes. At least one of the tethered enzymes is an enzyme that produces luminescence. The tethered enzymes are adapted to react to a constituent which may be a biomarker. A photonic luminescence reader measures light emitted from an active enzyme biomarker present in the patient's blood.

A sampling detection device includes an outer tube, an inner tube, and a tube cover, where the outer tube includes a reaction liquid holding chamber and a main body holding chamber that are communicated with each other; an end of the main body holding chamber is open; a side wall of the main body holding chamber includes a transparent structure; the inner tube includes a squeezing part and a test strip mounting part; the outer tube is configured to hold the inner tube; the test strip mounting part is located inside the main body holding chamber; the squeezing part is inserted into the reaction liquid holding chamber; the squeezing part cooperates with an inner wall of the reaction liquid holding chamber to form a gap; the tube cover is detachably connected to the outer tube and configured to close or open an open side of the main body holding chamber.

The invention provides an immunochromatographic test strip for quantitatively detecting monkey pox virus based on fluorescent latex microspheres, which takes europium chelate Eu (DBM) 3Phen-loaded monodisperse carboxylated polystyrene copolymer microspheres as a signal substance, and the microspheres are subjected to acid-base treatment before loading to loosen the surface structure of the microspheres so as to improve the loading capacity; the surface compound of the test strip is prepared by coupling carboxyl on the surface of the microsphere with an antibody. The invention also discloses a preparation method of the test strip. The kit is convenient for rapidly and accurately detecting the monkey pox virus, and has the characteristics of simplicity, rapidness, no toxicity and low cost.

The invention provides application of high-concentration colloidal gold in preparation of an influenza virus detection test strip, and relates to the technical field of immunodetection. The detection test strip provided by the invention is composed of a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a bottom plate, and in the preparation process of the detection test strip, high-concentration colloidal gold with the OD value of 100 is adopted for respectively marking an influenza A virus monoclonal antibody 1, an influenza B virus monoclonal antibody 1 and a chicken IgY antibody; optimized colloidal gold labeled reconstitution fluid is mixed and then sprayed on the combination pad, and meanwhile, a corresponding capture antibody and a goat anti-chicken IgY antibody are respectively sprayed on the nitrocellulose membrane to form a detection line and a quality control line. Through the synergistic effect of the high-concentration colloidal gold mark, the optimized sample pad / combination pad treating fluid and the special sample diluent, the detection sensitivity is remarkably improved, the false negative rate is effectively reduced, and rapid, high-sensitivity and high-specificity field detection on influenza A and B viruses is realized.

The invention discloses a dry-type electrochemical luminescence (ECL) lateral flow chip and application thereof in CRISPR-Cas detection. The dry-type ECL lateral flow chip comprises a lateral flow test strip and an electrode plate, the lateral flow test strip comprises more than one detection test strip, one electric connection test strip and at least one quality control test strip; the test strip sequentially comprises an activation sheet, a combination sheet, a detection sheet, an absorption sheet and a common sample sheet, the test strip forms radial branches taking the sample piece as the center; the electrode plate comprises an integrated closed bipolar electrode and a driving electrode; the shape of the integrated closed bipolar electrode is the same as that of the lateral flow test strip. According to the invention, the CRISPR-Cas system is freeze-dried on the test strip, so that accurate multiple detection of pathogens can be realized; according to the method, the operation process is simplified, the detection time is greatly shortened, the operation error is reduced, the mutual interference among multiple detections is reduced, and the generation of false positive results is avoided.

The invention discloses a preparation method and application of an HPV16 / 18 type E6 / E7 protein multi-target detection test strip, and belongs to the technical field of biological immunodetection analysis. The detection test strip comprises a back plate, a sample pad, a combination pad, a nitrocellulose membrane and an absorption pad, AIE fluorescent microsphere labeled probes are distributed on the combination pad, and a capture antibody and an AIE solution are distributed on the nitrocellulose membrane. The preparation method comprises the following three steps: preparation of the nitrocellulose membrane, preparation of the combination pad and assembly of the test strip. The detection test strip can synchronously and quantitatively detect PV16 E6, HPV16 E7, HPV18 E6 and HPV18 E7, each target corresponds to four signal points, multi-target synchronous rapid detection is achieved, the linear range is wide, the detection limit is low, operation is easy and convenient, cost is low, and the detection test strip is expected to become a novel efficient and reliable on-site screening tool for cervical cancer and precancerous lesions.

The invention relates to the technical field of test strips, in particular to a human saliva pepsin fluorescence immunochromatography test strip and a preparation method thereof.The human saliva pepsin fluorescence immunochromatography test strip is characterized in that a sample pad, a marking pad, a nitrocellulose membrane and a water absorption pad are pasted to the upper surface of a PVC plate, and the surface of the marking pad is sprayed with a pepsin marked antibody compound containing fluorescent microsphere marks; the fluorescence immunochromatography test strip provided by the invention can be used for specific detection of human saliva pepsin antigen, has a wide linear range, high sensitivity and high precision, can maintain the test value of the test strip to be basically unchanged for 14 days through accelerated destruction at 37 DEG C, shows good stability, and can be applied to detection of human saliva pepsin antigen. Meanwhile, the test strip is not interfered by amylase and lipase and has relatively high specificity, in addition, a method for detecting human saliva pepsin by adopting a fluorescence immunochromatography method has the advantages of operation detection, less time consumption, small matched equipment, low cost and the like, can meet the requirement of instant detection and has relatively high application value.

The invention discloses a hapten and an artificial antigen for detecting chlorfenapyr and application of the hapten and the artificial antigen. A time-resolved fluorescent test strip for detecting chlorfenapyr is prepared from the artificial antigen. The kit has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like, the quantitative detection limit of chlorfenapyr is as low as 3.48 ng / mL, the addition recovery rate is 99.6%-110.4%, the coefficient of variation is 2.1%-10.9%, and the kit is very suitable for rapid qualitative or quantitative detection of chlorfenapyr in plant-derived food by basic field personnel.

The invention relates to a dual ERA-LFD kit for visually and rapidly detecting salmonella typhimurium or a single-phase variant thereof, a primer group and application of the dual ERA-LFD kit and the primer group, and aims at solving the problems that an existing detection method is complex in operation, long in time consumption and depends on professional equipment, and primer cross interference, high false positive rate and incapability of distinguishing the single-phase variant caused by the fact that dual ERA primer design is not standard. Screening to obtain a specific primer probe combination aiming at the fljB gene deletion region and the STM4495 gene; a dual ERA-LFD detection system is constructed, and double targets are synchronously amplified under a constant temperature condition; visual interpretation is realized through a double-detection-line color development mode of the transverse flow test strip. The detection specificity can be remarkably improved, and false positive is effectively avoided; the accurate distinguishing between the salmonella typhimurium and the single-phase variants is realized; the detection process is rapid and simple, does not need professional instruments and personnel, and is suitable for on-site rapid screening; the detection cost is obviously reduced and the basic application feasibility is improved.

A point-of-care device and method for simultaneously and quantitatively assessing a magnetically-labelled analyte immobilised on a lateral flow test strip are provided. The method comprises the steps of sensing a magnetic signal from the magnetically-labelled analyte on the test strip and converting the magnetic signal into an electric signal; processing the electric signal to obtain a quantitative value of the amount of analyte; and processing the values to provide a differential assessment between the magnetically-labelled analyte. The device includes a magnetic reader with at least one magnetic sensor, such as a giant magnetoresistive sensor. The magnetic sensor can be directly or indirectly coupled to a lock-in amplifier configured to perform phase-sensitive detection and enhance the signal-to-noise ratio of weak magnetic signals. A system, microfluidics device, lateral flow immunoassay test strip and kit are also provided.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for detecting cat Cys-C protein and application. The monoclonal antibody combination provided by the invention comprises monoclonal antibodies 4D5 and 3F3, and amino acid sequences of complementary determining regions (CDR) of variable regions of a heavy chain and a light chain of the monoclonal antibodies 4D5 and 3F3 are respectively shown as SEQ ID NO.1 to SEQ ID NO.12. The antibody combination has high specificity and sensitivity, and can effectively recognize cat Cys-C recombinant protein and natural cat Cys-C protein. According to the double-antibody sandwich ELISA detection method constructed based on the antibody combination, positive signals can still be generated under the low concentration of 1ng / mL, no obvious cross reaction exists, and the detection accuracy is remarkably improved. The antibody combination can be used for preparing a detection kit, a test strip, an antibody chip and other tools, and is suitable for cat kidney function evaluation and early auxiliary diagnosis of kidney diseases.