1860 results about "Test strips" patented technology

The invention belongs to the application of a computer technology in the field of shot blasting processing, and discloses a shot blasting strength calculation method and system based on control parameters of intelligent shot blasting equipment. According to the method, an intelligent calculation method system is constructed by establishing a prediction model of the control parameters and the shot blasting strength. According to the method, nozzle pressure, moving speed, spraying angle, shot flow and target distance parameters of shot blasting equipment are collected in real time, workpiece material attribute data are combined, a multi-dimensional data fitting algorithm is adopted for processing, and an accurate shot blasting strength value is output. Meanwhile, a self-adaptive parameter adjusting module is developed, and dynamic calculation optimization can be carried out according to residual stress field distribution and coverage rate requirements. Compared with a traditional Almen test piece method, the method achieves real-time calculation of shot blasting strength and closed-loop control of technological parameters, the calculation error rate is reduced to be within 5%, test piece consumption is reduced by 80% or above, machining efficiency and quality stability are remarkably improved, and an innovative solution is provided for intelligent manufacturing in the field of surface strengthening.

The invention provides a test strip for rapidly detecting methicillin-resistant staphylococcus aureus and application of the test strip, and belongs to the technical field of biochemical detection. The test strip comprises a bottom plate, a sample pad, a combination pad, a detection pad and a water absorption pad, the two ends of the detection pad are in lap joint with the combination pad and the water absorption pad respectively, the detection pad is located on the lower portion, the sample pad is in lap joint with the combination pad, and the combination pad is located on the lower portion; a detection line and a quality control line are arranged on the detection pad, the detection line is arranged on one side close to the combination pad, and the quality control line is arranged on one side close to the water absorption pad; the detection pad is coated with a MoS2 nanosheet, the combination pad is coated with a penicillin binding protein 2a polyclonal antibody labeled by horse radish peroxidase, and the detection line is coated with a penicillin binding protein 2a monoclonal antibody; the quality control line is coated with goat anti-rabbit IgG (immunoglobulin G). The kit is high in sensitivity and wide in linear range, has good specificity, repeatability and stability, and can effectively detect MRSA in a clinical sample.

The invention discloses a test strip for five-item combined detection of inflammation of peripheral blood as well as a preparation method and application of the test strip. The test strip comprises a PVC bottom plate, a nitrocellulose membrane, a glass cellulose membrane combination pad, a sample pad and a water absorption pad, the PVC bottom plate is located at the bottommost part, and the sample pad, the glass cellulose membrane combination pad, the nitrocellulose membrane and the water absorption pad are sequentially arranged above the PVC bottom plate; the nitrocellulose membrane is provided with an HBP, PCT, IL-6, CRP and SAA detection line and a mouse anti-DNP monoclonal antibody quality control line; the glass nitrocellulose membrane combination pad is provided with a combination of a fluorescent microsphere-labeled HBP antibody, a fluorescent microsphere-labeled PCT antibody, a fluorescent microsphere-labeled IL-6 antibody, a fluorescent microsphere-labeled CRP antibody, a fluorescent microsphere-labeled SAA antibody and a fluorescent microsphere-labeled DNP-BSA. According to the test strip, fluorescence immunochromatography is carried out by utilizing the principle of a double-antibody sandwich method in combination with the characteristics of fluorescent microspheres, and rapid, specific, simple, convenient and sensitive joint detection on HBP, PCT, IL-6, CRP and SAA is realized at the same time.

The invention discloses a methyl paraoxon pesticide detection test strip based on oxygen vacancy-cerium dioxide and a preparation method of the methyl paraoxon pesticide detection test strip, and belongs to the technical field of biosensors. The test strip is characterized in that a large amount of oxygen vacancy-cerium dioxide is loaded. The oxygen vacancy-cerium dioxide nano enzyme can show simulated phosphatase activity under specific conditions, and the enzyme activity can cut off P-O and P-S bonds of organophosphorus pesticides and promote methyl paraoxon to be hydrolyzed into a yellow product p-nitrophenol. The detection limit of a nano-enzyme system is discussed, so that the portable visual quantitative detection of the paraoxon methyl pesticide is realized. The test strip established on the basis of the principle can quickly detect paraoxon methyl, has the advantages of simplicity and convenience in operation, high sensitivity, low cost and the like, and provides a new method for instant test of paraoxon methyl pesticide.

The invention provides a 25-hydroxyvitamin D dissociation and detection kit and a detection method thereof. By screening and optimizing formulas of a dissociation agent and antibody dry powder, automatic uniform mixing and dissociation of a sample are realized, the method is not influenced by human factors and does not depend on any uniform mixing equipment, and sufficient reaction of a 25-hydroxyvitamin D antibody and an object to be detected is ensured; meanwhile, the operation process of an immunochromatography method is improved, the dissociation process of a sample and the binding process of the 25-hydroxyvitamin D antibody and a to-be-detected substance are both completed in one reaction tube and are completely separated from a test strip, after the reaction is completed, the test strip is adopted for sample injection detection, meanwhile, a specific quality control line is arranged, and the detection accuracy is improved. The accuracy and the sensitivity of portable detection of the 25-hydroxy vitamin D by the immunochromatography are remarkably improved, and the detection sensitivity of the 25-hydroxy vitamin D reaches 1ng / mL.

A specimen collection, storage, transport, and testing device can include a vessel carrying a liquid reagent. A lid sealing the vessel can include a solid specimen collection stick projecting from an inside surface of the lid. Further, the lid can include a port on its outside surface. The port can be releasably engaged by a hand-manipulable handle facilitating specimen collection and attachment of the lid to the vessel. The port can further include an openably sealed aperture for accommodating the insertion of a test strip-carrying cartridge. The reusability of the handle and its reduced material structure helps avoid waste.

The invention discloses a micro-plastic integrated intelligent detection device, which comprises: a sample pretreatment module, which comprises a sample mixing module, a filtering separation module, an enrichment concentration module and a surface modification and marking module which are communicated in sequence and is used for mixing, filtering, enriching, modifying and marking a sample in sequence; the immunoreaction module is used for performing immunoreaction on the sample and the anti-polystyrene monoclonal antibody freeze-dried product marked by the quantum dot fluorescent microspheres; the fluorescence detection module is used for carrying out fluorescence detection on the test strip subjected to the immune reaction; the automatic control module is electrically connected with the sample pretreatment module, the immunoreaction module and the fluorescence detection module and is used for controlling the operation of each module. According to the invention, the sample processing module, the reaction module, the detection module and the control module are in close cooperation, the sample enters each module in sequence after simple pretreatment, detection and result output are semi-automatically completed, frequent manual operation of different instruments is not needed, the operation steps and time are reduced, and the operation difficulty is reduced.

The invention relates to the technical field of bacterium detection, in particular to a colorimetric-Raman coding immunochromatography test strip based on a 3D film-shaped SERS probe and a multi-bacterium detection method thereof. According to the technical scheme, the kit comprises a sample pad, a nitrocellulose membrane, a water absorption pad, a 3D membrane-like SERS probe and an operation buffer solution. According to the present invention, the immunochromatography of the 3D film-like probe is provided, the MoDAu-coated Ag SERS nano-film is adopted as the probe in the system, such that the pathogenic bacteria can be highly specifically recognized and captured, the immune complex fluidity can be increased, the rapid quantitative detection of the three pathogenic bacteria can be achieved, and the flexible 3D film-like SERS probe capable of enhancing the colorimetric ability and the SERS activity and highly specifically capturing the pathogenic bacteria can be prepared; by combining with an antibody-modified ICA test strip, rapid, simultaneous and quantitative determination of various pathogenic bacteria is realized.

The invention relates to a centrifugal micro-fluidic chip for automatic detection of a nano-enzyme test strip and application of the centrifugal micro-fluidic chip. The centrifugal micro-fluidic chip integrates the steps of sample adding, chromatography, color development and the like of the nano-enzyme test strip, and full automation of nano-enzyme experiments on the micro-fluidic chip is realized. Comprising a reaction structure main body, a bottom runner layer, a vertical through hole layer, a pressing layer, a top runner layer and a sealing body. The closed structure of the chip effectively reduces the interference of external environmental factors on the experiment, reduces the risk of sample pollution, and ensures the high reliability of the detection result.

The invention relates to the technical field of biological detection, and particularly discloses a test strip and a kit for detecting Alzheimer-related neurofilament protein AD7c-NTP. The detection test strip for the Alzheimer-related neurofilament protein AD7c-NTP provided by the invention comprises a PVC (Polyvinyl Chloride) bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad are arranged on the PVC bottom plate; a mouse anti-AD7c-NTP monoclonal antibody I marked by latex is contained on the combination pad; a detection line T and a quality control line C are arranged on the nitrocellulose membrane; the detection line T is coated with a mouse anti-AD7c-NTP monoclonal antibody II, and the quality control line C is coated with a goat anti-mouse IgG polyclonal antibody. The detection test strip for Alzheimer-related neurofilament protein AD7c-NTP provided by the invention has the advantages of high sensitivity, strong specificity, good stability and the like, can realize auxiliary diagnosis of Alzheimer's disease, and has a wide application prospect.

The invention relates to the technical field of pesticide residue detection, and discloses an all-in-one fluorescent quantitative test strip management system and method based on a block chain, and the method comprises the steps: obtaining pesticide residue detection data of an all-in-one fluorescent quantitative test strip, carrying out the chaining storage of the detection data, and constructing a pesticide residue toxicity knowledge graph; comparing the detection data with the knowledge graph, and determining the original risk level of the pesticide; in combination with the region and time information, other detection data of the same region and the same time period in the block chain are obtained, clustering analysis is executed, and a pesticide risk reference of the region is determined; dynamically calibrating the original risk level based on the risk reference to generate a calibrated risk level; setting an intelligent contract on the block chain, and automatically triggering a corresponding execution strategy according to the calibrated risk level; the pesticide residue risk can be objectively and dynamically evaluated, intelligent management of pesticide residues is achieved, and the food safety guarantee level of agricultural products is greatly improved.

The utility model relates to a test strip detection area and a test strip substrate matched with the test strip detection area. The test strip detection area is used for detecting physiological indexes in samples such as blood. According to the technical scheme, a first reaction layer is located on a first electrode layer and provided with a first reaction area; the channel layer is positioned on the first reaction layer; the test strip detection area is used for being mounted on a test strip substrate to form a test strip, and the second reaction layer is positioned on the channel layer and is provided with a second reaction area; the second electrode layer is located on the second reaction layer; the covering layer is positioned on the second electrode layer; sample channels are formed in positions, corresponding to the first reaction region and the second reaction region, on the channel layer. The test strip has the advantages that the test strip is of a split structure, even if unqualified products appear in the test strip detection area in the liquid dispensing process, the unqualified test strip detection area can be scrapped, the test strip substrate does not need to be scrapped, and compared with the prior art, the production cost is greatly reduced.

The invention discloses test paper and a kit for detecting urea in body fluid, and the test paper comprises a base layer which comprises a near end and a far end transversely extending from the near end; the sampling area is arranged at the far end of the base layer and is used for absorbing body fluid to be detected; the reaction area is arranged on the base layer and is used for absorbing the body fluid in the sampling area so as to react by utilizing urease in the reaction area; the diffusion area is arranged on the base layer, is connected with the reaction area and is used for performing lateral chromatography on reactants generated in the reaction area from the far end to the near end; the color development area is arranged on the base layer, is isolated from the reaction area through the diffusion area and is used for absorbing reactants subjected to lateral chromatography by the diffusion area so as to carry out color development reaction by utilizing color development substances in the color development area, so that a detection result of the urea in the body fluid is displayed.

A station for a urine analysis device is proposed, the station including a case, intended to be positioned at least partially within the toilet, a housing, within the case, configured to at least partially receive a cartridge comprising a plurality of test supports (e.g. test strips). The cartridge is movable within the housing. The station may also include an optical sensor, a translatory injector and an analyzer (e.g. optical). Various configurations are available.

The invention belongs to the technical field of microbiological detection, and particularly relates to a closed nucleic acid extraction, amplification and detection fully-integrated device. Comprising an injector type nucleic acid extraction, enrichment and amplification assembly, a detection card box, a three-way valve and a sealing cover, wherein the nucleic acid extraction, enrichment and amplification assembly comprises a chitosan modified paper sheet used for nucleic acid enrichment and an injector, the chitosan modified paper sheet is fixed in a needle cylinder of the injector through a rubber plug, and a piston of the injector is isolated from the chitosan modified paper sheet through isolation liquid; the detection card box is internally provided with at least one test strip, a box body is provided with a vent hole, the vent hole is covered with a film, and the film is breathable and waterproof; when an amplification product is loaded, one end of the three-way valve is connected with the detection card box, the other end of the three-way valve is connected with the nucleic acid extraction, enrichment and amplification assembly, and the third end of the three-way valve serves as a liquid inlet; during detection, the sealing cover is connected with the third end of the three-way valve.

The invention relates to the technical field of pesticide detection, and discloses a multi-component colloidal gold test strip for rapid detection of organophosphorus as well as a preparation method and application thereof, the preparation method comprises the following steps: preparing a specific antibody of the organophosphorus, and preparing a colloidal gold solution at the same time; mixing and incubating the specific antibody and a colloidal gold solution to obtain an antibody-labeled colloidal gold solution; pre-treating a detection pad, and immersing the detection pad in the antibody-labeled colloidal gold solution to obtain an impregnated pad; and sequentially assembling the dried dipping pad, a water absorption pad, a sample pad and a combination pad on a bottom plate to form a complete test strip. By improving the preparation process of the antibody and the assembly technology of the test strip, the detection sensitivity and specificity are remarkably improved. The test strip is simple and convenient in preparation process, high in cost benefit and convenient for large-scale production. In addition, the test strip is convenient to use and does not need to depend on professional equipment or technicians, and the detection result is visual and convenient to read.

The invention relates to a pesticide residue detection test strip as well as a preparation method and application thereof, in particular to a rapid detection technology based on an enzyme inhibition method and a chitosan non-woven fabric carrier. The test strip can specifically detect organophosphorus and carbamate pesticides such as trichlorfon, malathion and phoxim through the enzyme activity inhibition principle, the detection limit of the pesticides such as phoxim is as low as 0.1 mg / kg, the developing intensity is in negative correlation with the pesticide concentration, and semi-quantitative judgment can be achieved. The preparation process is simple, the chitosan non-woven fabric is adopted as a fixed carrier, the material is environment-friendly and degradable, the enzyme activity recovery rate reaches 90%, the stability is excellent (the enzyme activity recovery rate can be preserved for 60 days at the temperature of 4 DEG C in a dark place), sample pretreatment is not needed in detection operation, a result can be obtained at the normal temperature for 15 min, the stability and repeatability are good, and the problems that a traditional instrument is high in detection cost and long in time consumption are solved; the method has a remarkable basic popularization value.

The invention belongs to the technical field of biology, and relates to a latex immunochromatography test strip for rapidly detecting porcine epidemic diarrhea virus (PEDV) and a preparation method of the latex immunochromatography test strip. The test strip comprises a PVC (polyvinyl chloride) bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, a quality control line C and a detection line T are arranged on the nitrocellulose membrane. A latex microsphere labeled PEDV N protein monoclonal capture antibody is sprayed on the combination pad, a detection line T is coated with a PEDV N protein monoclonal detection antibody 1E1, and a quality control line C is coated with goat anti-mouse IgG. The test strip can specifically recognize PEDV, and does not react with other porcine intestinal coronaviruses with similar clinical symptoms. The test strip is good in specificity, relatively high in sensitivity and simple to operate, a result is interpreted within 10 minutes, and compared with a traditional colloidal gold detection technology, the stability and the color development effect are obviously improved.

Disclosed is a multiplex quantum dot-based quantitative test card, and a preparation method, apparatus, and use method thereof in the technical field of biological testing, the multiplex quantum dot-based quantitative test card including a multiplex card housing and a test strip, where the multiplex card housing is provided with a plurality of reaction slots configured to accommodate the test strip; the multiplex card housing further includes a plurality of sample loading ports and a plurality of observation windows, where the sample loading ports and the observation windows are in one-to-one correspondence with the reaction slots; and the test strip includes a conjugate pad coated with quantum dot-labeled antibodies, where the quantum dot-labeled antibodies serve as chromogenic substances.

The invention belongs to the technical field of molecular diagnosis, and particularly discloses a high-risk HPV16 rapid detection system based on RPA-CRISPR / Cas12a, which is prepared by the following steps: (1) mixing a nucleic acid lysis solution containing Tris (pH = 8.0), NaHCO3, polyvinylpyrrolidone and Tween-20 with Proteinase K to treat a sample, and performing pyrolysis at room temperature for 5 minutes to obtain a nucleic acid template; (2) an RPA reaction system contains an RNA inhibitor, a reverse transcriptase and an RPA reaction essential enzyme, and after the template and the starting agent in the step (1) are added, RPA amplification is carried out at 37 DEG C; (3) dropping the reaction liquid on a colloidal gold test strip subjected to position exchange and transformation of a sample pad and a water absorption pad, and carrying out result interpretation; and (4) adding the RPA product into a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) reaction system containing the LbCas12a protein, the specific gRNA and the probe. According to the scheme, a rapid, high-sensitivity and cross-reaction-free detection system of the HPV16 is realized by optimizing a nucleic acid lysis solution formula, designing a specific primer and gRNA and modifying a test strip for detection.

The invention discloses a colloidal gold test strip for distinguishing porcine actinobacillus pleuropneumoniae wild virus infection and vaccine immunity and application. According to the test strip, ApxIVA truncated protein with the amino acid sequence shown as SEQ ID NO.3 serves as an antigen, the truncated protein can be subjected to soluble expression in escherichia coli, and the structure and the function of the truncated protein are close to those of natural apxIVA. The test strip comprises a sample pad, a colloidal gold pad, an NC membrane, water absorption filter paper and a bottom plate, the colloidal gold pad is coated with gold-labeled apxIVA truncated protein and a gold-labeled chicken IgY antibody, and a goat anti-chicken IgY antibody and apxIVA truncated protein are fixed on a C line and a T line of the NC membrane. The method can be used for distinguishing APP wild strain infection and gene deletion seedling APP-HB-04M immune serum samples.

The invention discloses a test strip for detecting etoxazole and application of the test strip. The test strip comprises a sample absorption pad (1), a conjugate release pad (2), a reaction film (3), a water absorption pad (4) and a bottom plate (7), the reaction film is provided with a detection line (5) coated with an etoxazole hapten-carrier protein conjugate and a quality control line (6) coated with a goat anti-mouse antibody, and the conjugate release pad (2) is sprayed with an etoxazole monoclonal antibody-colloidal gold marker. The invention also provides a method for detecting residual etoxazole in vegetables and fruits by using the etoxazole test strip. The test strip provided by the invention has the characteristics of simplicity in operation, high sensitivity, high detection speed, low cost and the like, and is suitable for screening and on-site monitoring of a large number of samples.

The self-service AIDS detection device comprises a detection box, an auxiliary detection unit, detection test paper, an observation window, a switch button and a blood sampling assembly, the auxiliary detection unit is fixedly installed in the detection box, and the bottom of the detection test paper is fixedly installed at the inner bottom of the detection box. According to the invention, self-help AIDS detection is carried out, the first carriage and the second carriage move back and forth in the opposite direction or the opposite direction, the first carriage and the second carriage move to drive the connecting plate, and then the connecting plate drives the finger squeezing curved plate to squeeze the fingers, so that the punctured fingers continuously flow blood; a displacement toothed plate, a cylindrical gear pair, a bevel gear pair, a transmission shaft rod and an elliptical block are driven to rotate, and the elliptical block rotates to enable a first nitrocellulose membrane and a second nitrocellulose membrane to move in an opening and closing manner, so that blood and a blood diluent are effectively mixed; the mixed liquid flows to the detection test paper along the guide conical cylinder and the guide cylinder, and the detection test paper is used for detecting AIDS.

The invention discloses beryllium element detection test paper as well as a preparation method, a detection method and application thereof. The beryllium element detection test paper comprises a filter paper base material, a color developing agent chrome azurol S and a shielding agent ethylene diamine tetraacetic acid disodium salt, the color developing agent chrome azurol S and the shielding agent disodium ethylene diamine tetraacetate are loaded on the filter paper base material. When the beryllium element detection test paper is used for detecting a surface beryllium pollution detection object, the detection method is simple and rapid to operate, high in sensitivity and low in cost.

The invention discloses a hybridoma cell strain capable of secreting clonorchis sinensis monoclonal antibodies and application of the hybridoma cell strain, and belongs to the technical field of monoclonal antibodies. The invention aims to improve the sensitivity, specificity and titer of clonorchis sinensis monoclonal antibody detection. The invention provides a hybridoma cell strain capable of secreting clonorchis sinensis monoclonal antibodies, the hybridoma cell strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.46128, and the preservation time is November 6, 2024. The test strip provided by the invention is safe and harmless to an operator and does not cause pollution to the environment, so that the test strip has huge application potential and prospect in the market of clinical detection of clonorchiasis sinensis.

The invention provides a pepsin detection card and a preparation process thereof, and belongs to the technical field of microbiological detection. The kit comprises a test strip and a card shell, the test strip comprises a nitrocellulose membrane, a sample pad, absorbent paper, a combination pad and a bottom plate. The pepsase detection card is high in sensitivity, can accurately capture pepsase with extremely low concentration in a sample, effectively avoids missed diagnosis, strives for precious time for early intervention, is high in specificity, is only specifically combined with pepsase, is not interfered by other similar proteases or impurities in the sample, and can be used for detecting the pepsase in the sample. In a complex human body biological sample environment, the target pepsin can still be accurately recognized, the specificity reaches 98% or above, false positive results are greatly reduced, and a reliable basis is provided for clinical diagnosis.

According to the preparation and application of the novel Fe monatomic nano enzyme, mesoporous silica is used as a substrate material, the surface of the substrate material is coated with a layer of dopamine and hemin, then high-temperature calcination is performed to form the monatomic nano enzyme Fe-N-C, then the monatomic nano enzyme Fe-N-C is used as a signal reporter molecule of a lateral flow chromatography test strip, and the application of the monatomic nano enzyme Fe-N-C in the lateral flow chromatography test strip is realized. A labeled antibody is connected to the surface of the antibody, so that rapid detection of cardiac troponin I (cTnI) is realized. In the monatomic nano-enzyme, dopamine mainly provides a carbon source, heme mainly provides Fe and a nitrogen source, the larger specific surface area of mesopores provides abundant sites for the monatomic nano-enzyme, and the contact area of the monatomic nano-enzyme and a substrate is increased, so that the enzyme activity is improved. In conclusion, the material has a large specific surface area and relatively high enzyme activity. And finally, quickly detecting the biomarker cTnI after the test strip platform is integrated.

The invention discloses a lateral flow chromatography test strip for detecting perfluorooctanoic acid by combining gold nanoparticles, a nucleic acid aptamer and an antibody. According to the test strip, a nucleic acid aptamer marked by gold nanoparticles is combined with PFOA molecules to form a nucleic acid aptamer with a G-quadruplex structure, and then the nucleic acid aptamer with the G-quadruplex structure is specifically recognized and captured by an anti-BG4 antibody, so that specific visual detection of perfluorooctanoic acid is realized. After a sample is injected, a G-quadruplex structure formed by the PFOA and the nucleic acid aptamer acts with the anti-BG4 antibody coated on the test strip, so that the gold nanoparticles are agglomerated to generate a chromogenic reaction, the signal intensity can be detected by naked eyes or reading equipment, the visual detection limit is 10 mu M, and the equipment detection limit is 304 nM. The test strip has the advantages of high detection speed, high specificity and high sensitivity, and is suitable for field detection and rapid screening. Compared with a traditional method, the method realizes simple, convenient and economical detection of PFOA, and has a wide application prospect.

The invention relates to methods, devices, and software for distinguishing hemoglobin (Hb) from myoglobin (Mb) in urine samples using peroxidase-reactive test strips. A time-resolved video of the color change on the test pad is recorded, and hue values are extracted and analyzed over time. Diagnostic differentiation between Hb and Mb is achieved by evaluating the hue-time curve, in particular via endpoint hue values and the ratio of integrated hue values in distinct time intervals. The invention further comprises a standardized optical setup for recording, a software-based evaluation algorithm, and a refillable cartridge for in-situ urine analysis devices containing appropriate test fields. The invention enables combined testing with additional urine parameters and is suitable for medical and non-clinical applications such as sport and home diagnostics.

A test strip for detecting uric acid in saliva that meets the requirement for room-temperature preservation, a preparation and detection method therefor and the use thereof. The test strip for detecting uric acid in saliva comprises a uricase, a peroxidase, a vitamin C oxidase, a bilirubin oxidase, a color developing agent, and a protective film coated on the test strip for detecting uric acid in saliva. To meet the needs of a target population for long-term monitoring and real-time tracking of changes in their uric acid levels, by means of agent addition, the test strip for detecting uric acid in saliva has prolonged preservation time at room temperature, enables non-invasive, efficient and convenient monitoring of a health status at any time, and timely detection and treatment, allows intuitive visual identification of uric acid levels in saliva by a subject, and meets the needs of a subject for long-term preservation at room temperature.