511 results about "Test strips" patented technology

The invention relates to a pesticide residue detection test strip as well as a preparation method and application thereof, in particular to a rapid detection technology based on an enzyme inhibition method and a chitosan non-woven fabric carrier. The test strip can specifically detect organophosphorus and carbamate pesticides such as trichlorfon, malathion and phoxim through the enzyme activity inhibition principle, the detection limit of the pesticides such as phoxim is as low as 0.1 mg / kg, the developing intensity is in negative correlation with the pesticide concentration, and semi-quantitative judgment can be achieved. The preparation process is simple, the chitosan non-woven fabric is adopted as a fixed carrier, the material is environment-friendly and degradable, the enzyme activity recovery rate reaches 90%, the stability is excellent (the enzyme activity recovery rate can be preserved for 60 days at the temperature of 4 DEG C in a dark place), sample pretreatment is not needed in detection operation, a result can be obtained at the normal temperature for 15 min, the stability and repeatability are good, and the problems that a traditional instrument is high in detection cost and long in time consumption are solved; the method has a remarkable basic popularization value.

The invention discloses a test strip for detecting etoxazole and application of the test strip. The test strip comprises a sample absorption pad (1), a conjugate release pad (2), a reaction film (3), a water absorption pad (4) and a bottom plate (7), the reaction film is provided with a detection line (5) coated with an etoxazole hapten-carrier protein conjugate and a quality control line (6) coated with a goat anti-mouse antibody, and the conjugate release pad (2) is sprayed with an etoxazole monoclonal antibody-colloidal gold marker. The invention also provides a method for detecting residual etoxazole in vegetables and fruits by using the etoxazole test strip. The test strip provided by the invention has the characteristics of simplicity in operation, high sensitivity, high detection speed, low cost and the like, and is suitable for screening and on-site monitoring of a large number of samples.

The invention provides application of high-concentration colloidal gold in preparation of an influenza virus detection test strip, and relates to the technical field of immunodetection. The detection test strip provided by the invention is composed of a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a bottom plate, and in the preparation process of the detection test strip, high-concentration colloidal gold with the OD value of 100 is adopted for respectively marking an influenza A virus monoclonal antibody 1, an influenza B virus monoclonal antibody 1 and a chicken IgY antibody; optimized colloidal gold labeled reconstitution fluid is mixed and then sprayed on the combination pad, and meanwhile, a corresponding capture antibody and a goat anti-chicken IgY antibody are respectively sprayed on the nitrocellulose membrane to form a detection line and a quality control line. Through the synergistic effect of the high-concentration colloidal gold mark, the optimized sample pad / combination pad treating fluid and the special sample diluent, the detection sensitivity is remarkably improved, the false negative rate is effectively reduced, and rapid, high-sensitivity and high-specificity field detection on influenza A and B viruses is realized.

A point-of-care device and method for simultaneously and quantitatively assessing a magnetically-labelled analyte immobilised on a lateral flow test strip are provided. The method comprises the steps of sensing a magnetic signal from the magnetically-labelled analyte on the test strip and converting the magnetic signal into an electric signal; processing the electric signal to obtain a quantitative value of the amount of analyte; and processing the values to provide a differential assessment between the magnetically-labelled analyte. The device includes a magnetic reader with at least one magnetic sensor, such as a giant magnetoresistive sensor. The magnetic sensor can be directly or indirectly coupled to a lock-in amplifier configured to perform phase-sensitive detection and enhance the signal-to-noise ratio of weak magnetic signals. A system, microfluidics device, lateral flow immunoassay test strip and kit are also provided.

This invention belongs to the field of microcystin-LR detection technology, providing anti-microcystin-LR nanobodies and their applications. The anti-microcystin-LR nanobodies have amino acid sequences as shown in SEQ ID NO.1, SEQ ID NO.2, or SEQ ID NO.3. This invention uses phage display technology to screen for nanobodies with high affinity and high specificity for recognizing microcystin-LR, exhibiting advantages such as strong stability and ease of large-scale expression. Various detection tools can be designed based on anti-microcystin-LR, including test strips, immunoassay kits, immunoaffinity columns, biosensor detection chips, or detection probes. This provides novel antibody resources for rapid monitoring of microcystin-LR and lays a material foundation for developing low-cost, high-performance immunoassay technologies, which is of great significance for promoting the monitoring and control of microcystin-LR pollution.

The utility model discloses a detector test strip fixing module convenient for non-professionals to use, which relates to the technical field of fast detection detectors and comprises a base and an insertion piece. When the kit is used, the bottom end of a reagent strip is fixed in the fixing groove and is matched with the fixing block to fix the reagent strip, and during detection, the sampling rod punctures the thin sheet A between the solution cavity and the communication cavity from top to bottom, so that a diluent with a sample enters the communication cavity and reaches the other side of the insertion sheet through the through hole; due to the fact that the bottom of the reagent strip is fixed in the fixing groove and makes contact with the diluent passing through the through hole, detection is achieved, the diluent is blocked below the first water blocking baffle and does not make direct contact with other parts of the reagent strip, and the purpose that the diluent gradually seeps to the top of the reagent strip is achieved; and therefore, the operation convenience and the accuracy of a detection result during detection by a non-professional user are further improved.

This invention provides an assembled high-throughput immunochromatographic test strip array sample application device, comprising a 96-well plate fixing bracket (1) and a test strip support plate (2); immunochromatographic test strips (3) are arranged and fixed by slots (4) and limiting blocks (5) on the test strip support plate (2), with their sample application areas (6) protruding from the test strip support plate (2); multiple test strip support plates (2) are inserted into the slots (7) of the 96-well plate fixing bracket (1) to form a multi-channel immunochromatographic test strip array sample application device. This device can be used for high-throughput sample application in immunochromatographic detection.

The utility model discloses a blood detection device. The blood detection device comprises a shell, test paper and a cap, the shell comprises an upper shell and a lower shell which are fixedly connected, a containing cavity is formed between the upper shell and the lower shell, the test paper is located in the containing cavity, a blood sampling head is formed at the front end of the shell, a liquid inlet is formed in the blood sampling head, and the liquid inlet is communicated with the containing cavity through a capillary structure and a premixing cavity in sequence; a hydrophobic coating belt arranged in the left-right direction is arranged on the front cavity wall of the premixing cavity, and an air hole communicated with the containing cavity is formed in the rear end of the shell; the front end of the shell is sleeved with the cap, a liquid storage bag is arranged at the bottom in the cap, and buffer liquid is stored in the liquid storage bag. The blood detection device can realize integration of sample collection and sample treatment, and has the advantages of simple structure, low manufacturing cost, convenience in use, high detection success rate, high detection accuracy and the like.

The invention relates to a detection test strip selecting and overturning device which comprises a rack, a roller, a CIS sensor and a discharging mechanism. The roller is rotatably mounted in the rack, and a clamping groove for only taking out one detection test strip from the test strip storage bin at a time is formed in the roller; the CIS sensor is used for judging whether the front side or the back side of the detection test strip faces the outer side of the roller; the discharging mechanism pulls out the detection test strips with the front faces facing the outer side of the roller from the clamping grooves and then conveys the detection test strips downwards to the conveying platform, or the discharging mechanism pulls out the detection test strips with the back faces facing the outer side of the roller from the clamping grooves, turns over the detection test strips and then conveys the detection test strips downwards to the conveying platform. According to the invention, the turnover / turn-over function of the test strip is realized, the hidden danger caused by the damage or fault of the test strip in the turnover process is reduced, the operation continuity is great, the overall structure is relatively simple and compact, the working efficiency and reliability are improved, and the market competitiveness of the product in similar products can be improved.

The invention discloses a swelling microneedle patch and a preparation method thereof. The swelling microneedle patch comprises a backing and a swelling microneedle array adhered to the backing, the swelling microneedle array comprises a plurality of microneedles; the microneedle is in the shape of a cone or a rectangular pyramid; the height of the microneedle is 100 to 1000 [mu] m; the diameter of the tip end of the microneedle is 5-30 microns; the diameter of the bottom of the microneedle is 50-500 [mu] m; and the center-to-center distance between every two adjacent microneedles is 50-2000 microns. The swelling microneedle patch provided by the invention can overcome the barrier action of cuticle to penetrate subcutaneously and absorb subcutaneous tissue interstitial fluid, and is simple in preparation process, low in cost and high in universality. The patch is combined with a colloidal gold lateral chromatography test strip, so that the limitations that in-vitro diagnosis of infectious diseases depends on a blood sample, professional medical personnel are needed for operation, the subject compliance is poor and the like can be solved, and the patch is suitable for rapid screening and diagnosis of the infectious diseases.

This application discloses a sample analyzer and its control method. The analyzer includes a test strip providing device, a test strip transferring device, a sample application device, an information acquisition device, and a controller. The test strip includes a substrate, a detection area disposed on the substrate for dry chemical detection of the sample, a first identifier indicating manufacturer information, and a second identifier indicating test strip specifications. The controller controls the sample application device to apply the sample to the detection area of ​​the test strip for a dry chemical reaction, and controls the information acquisition device to acquire the detection information of the detection area, the manufacturer information of the first identifier, and the test strip specifications of the second identifier after the dry chemical reaction is completed. Based on the detection information, manufacturer information, and test strip specifications, the controller obtains and outputs the detection results, ensuring that each entered test strip information corresponds one-to-one with the currently used test strip, thus improving the accuracy of the detection results.

The present disclosure relates to a device for lateral flow testing of a liquid sample, the device comprising: a test strip comprising: a membrane having a first capture reagent disposed on a first surface at a test location, the first capture reagent configured to capture an analyte in the liquid sample; a sample pad disposed on the first surface at a first end of the membrane spaced apart from the test location, the sample pad configured to receive a liquid sample; and a conjugate pad disposed on the first surface between the sample pad and the test location, the conjugate pad having deposited thereon a catalytic labeling reagent configured to chemically bind to an analyte; an activation layer disposed over the membrane, arranged to contact the first surface upon activation, the activation layer having deposited thereon at least one component of a catalyst substrate capable of chemically reacting with a catalytic labeling reagent upon activation; and a support structure configured to attach the test strip and the active layer when the device is in an inactive state such that the active layer is separated from the membrane.

The utility model is designed for the rapid (up to 20 minutes) detection of at least 10 types of analytes in environmental samples (water, air, soil, etc.) and clinical specimens. The immunochromatographic test strip for multiplex analysis includes a polymer substrate with an applied immunochromatographic membrane and a capture pad. The test and control zones of the test strip are designed as two rows of transverse microstrips occupying no more than half the width of the test strip. The test strip includes a marker for coordinating the cutting of test strips. The technical result consists in reducing the time for recording the luminescent signal from test strips in an automated system. 3 c.p. phyl., 3 fig., 2 tables.

The invention relates to the technical field of biology, in particular to leukocyte esterase detection test paper and a preparation method thereof. Based on the principle of a substrate and a color developing agent, the color developing depth is in direct proportion to the concentration of the esterase, so that the concentration of the leukocyte esterase in a detection sample can be judged according to the color developing depth; meanwhile, under the auxiliary action of calcium chloride and other materials, the detection distinction degree, the detection accuracy and the detection result stability are further improved. The leukocyte esterase test paper provided by the invention is simple in sample detection method, more stable in product performance, longer in validity period and wide in application range, can be used as an auxiliary evaluation index for vagina inflammation, and is combined with a cleanliness index and the like to jointly evaluate the vagina environment.

The application discloses a lateral test strip for detecting C-reactive protein and application thereof, and the test strip comprises a bottom plate and a sample pad, a combination pad, a nitrocellulose membrane and a water absorption paper which are sequentially stacked on the bottom plate; the combination pad is loaded with AIE fluorescent microspheres; the AIE fluorescent microspheres are composed of P(St-MMA) microspheres coated with AIE materials after hydrolysis and carboxylation; and anti-C-reactive protein antibodies are connected to the AIE fluorescent microspheres through a covalent bond. The surface coating amount of the AIE fluorescent microspheres is increased by 1.6-2.3 times, and the fluorescence intensity per unit mass is increased by about 40-60%. The increase of the coating amount makes the density of the light-emitting core higher, the overall quantum yield is significantly improved, and the uniformity of the fluorescence signal and the detection sensitivity are enhanced. The characteristics are particularly prominent in the detection of CRP protein in the chromatographic test strip, and the detection limit can be effectively reduced, the T / C signal ratio can be improved, and the reaction time can be shortened.

The application discloses a monoclonal antibody of SARS-CoV-2 N protein and a preparation method and application of a test strip thereof. The red microsphere immunochromatography test strip prepared from the monoclonal antibody of SARS-CoV-2 N protein can quickly and accurately detect SARS-CoV-2 N protein, shortens the detection time, simplifies the detection steps, has the characteristics of being quick and convenient and being suitable for on-site detection, and provides a reliable immunological technology for quickly and efficiently detecting SARS-CoV-2 infection.

A rapid diagnostic device for detecting multiple biomarkers in bodily fluid samples, particularly useful for disease screening including breast cancer. The device comprises a housing containing at least one test strip with a sample receiving zone, conjugate pad, and multiple test zones. The test strip utilizes specific materials including Cytiva GF / DVA and Ahlstrom 1667 sample pads, an Ahlstrom 6613 conjugate pad with carboxylated 150 nm gold nanoshell conjugates, and a Sartorius CN140 membrane with precisely positioned test and control zones. The device simultaneously detects multiple biomarkers, such as hCG and CA-15-3, using specific antibodies. Features include a built-in safety lancet, integrated buffer system, and compatibility with various sample types. Results are available within ten minutes, with options for quantitative analysis via a dedicated reader or smartphone application. This multi-biomarker approach potentially improves screening accuracy compared to single-marker tests, offering versatility for detecting various medical conditions.

The application provides a glucose detection test strip and a preparation method and application thereof. The preparation method of the glucose detection test strip comprises the following steps: taking copper sulfate pentahydrate, a phosphate buffer solution, glucose oxidase and horseradish peroxidase, mixing uniformly to obtain an enzyme-containing mixed solution; immersing a test strip in the enzyme-containing mixed solution, and obtaining an organic-inorganic hybrid nanoflower loaded test strip after reaction; immersing the organic-inorganic hybrid nanoflower loaded test strip in a mixed solution containing a chromogenic agent, a gel, bovine serum albumin and polyethylene glycol to obtain a glucose detection test strip. The preparation method of the glucose detection test strip has higher sensitivity and shorter response time.

The invention provides an ichthyophthirius multifilis detection reagent and detection method based on an enzymatic isothermal amplification technology, and belongs to the technical field of pathogen detection. The invention provides a primer probe group for detecting ichthyophthirius multifilis by using an enzymatic isothermal amplification (ERA) method, and a CO1 gene of the ichthyophthirius multifilis is used as a target sequence. Based on the ERA primer probe group designed by the invention, a test strip type isothermal detection kit and a fluorescent isothermal detection kit are developed, and are respectively associated with'test strip type visual detection 'and'fluorescent quantitative detection'. The two ERA detection kits developed by the invention have the advantages of strong specificity, high detection sensitivity, high detection efficiency and no need of high-temperature equipment, and can meet the requirements of on-site rapid visual detection. According to the invention, the technical blank of on-site rapid diagnosis of ichthyophthirius multifilis is filled, and a key technical support is provided for early prevention and control of aquaculture diseases.

The application discloses a kind of test strip manufacturing method for simulating peel strength under inner layer substrate process flow, it belongs to substrate test technical field, it solves the problem that the test strip of cutting in prior art cannot simulate the actual substrate condition.It mainly includes: sampling two carrier copper foils, double-sided copper foil substrate is formed by once pressing treatment;Copper foil substrate upper and lower surface is carried out electroplating or micro-etching operation;Copper foil substrate upper and lower surface is carried out roughening treatment;Two inner layer substrates are placed on the upper and lower sides of copper foil substrate, and an integrated body is formed by twice pressing treatment;After separating out test substrate, copper foil on test substrate is generated pattern;Finally, test substrate is cut into test strip.The application can realize all designs of inner layer pattern in reality, such as electroplating, micro-etching copper reduction and other actual scene simulation, and the test strip manufactured by the method of the application can verify the actual peel strength of the process flow in actual production.

This invention relates to the field of biodetection technology, and more particularly to a fluorescent test strip for detecting canine parvovirus, along with hybridoma cells, monoclonal antibodies, and their applications. The test strip of this invention has fluorescent microspheres coated with canine parvovirus monoclonal antibody and fluorescently labeled rabbit IgG within the conjugate pad; a detection line and a control line are disposed on the surface of a nitrocellulose membrane, with the detection line coated with canine parvovirus monoclonal antibody and the control line coated with rabbit anti-IgG; wherein the canine parvovirus monoclonal antibody is secreted using hybridoma cells with accession number C202355. The test strip of this invention can be used to simultaneously detect feline parvovirus, canine parvovirus, and various genotypes of canine parvovirus, exhibiting high sensitivity and specificity, and possessing the technical advantages of accuracy, speed, and simplicity.

The present application relates to the field of immunochromatography, in particular to a sample pad treatment method, a detection method and a chromatography test paper for drug abuse detection, and discloses an immunochromatography test paper for drug abuse detection, which is characterized by a sample pad treatment liquid containing Pluronic F-127.

The invention provides a time-resolved fluorescence immunochromatography kit and a detection method for simultaneously detecting myocarine and ergodiethylamine. The kit mainly comprises a time-resolved fluorescent microsphere labeled antibody probe and a test strip, carrying out chemical coupling on the time-resolved fluorescent microspheres and a monoclonal antibody of the myocarine and the ergodiethylamine to prepare a time-resolved fluorescent microsphere labeled antibody probe; the test strip comprises a nitrocellulose membrane, a sample pad and an absorption pad which are arranged on a bottom plate, and the nitrocellulose membrane is respectively coated with detection lines T1 and T2 of a myocarine antigen and an ergodiethylamine antigen, and is coated with a quality control line C of a goat anti-mouse IgG antibody. The method can be applied to rapid screening of the maccarine and the ergodiethylamine in the hair sample, is simple to operate, rapid and convenient, accurate in quantification and high in sensitivity, and is suitable for various temporary detection scenes such as community drug rehabilitation, entertainment venues and public security checkpoints.

This utility model relates to the field of immunoassay technology and discloses a shake-resistant fluorescent immunochromatographic reagent kit, including a placement box and a detection box. The detection box contains a test strip, a sample pad, and an absorption pad. The top of the detection box has a sample dispensing port and a display area. A support base is installed at the center of the top of the placement box, and a limiting seat is installed at the inner edge of the placement box. Both the top of the support base and the limiting seat have six sets of limiting grooves. This shake-resistant fluorescent immunochromatographic reagent kit can be stably placed using the placement box, and the test reagent can be tightly embedded in the limiting grooves for fixation. When adding the fluorescent immunochromatographic solution, excess liquid can fall into the placement box for collection and prevent leakage. The anti-slip pad at the bottom of the placement box improves stability, and the detection box embedded inside prevents shaking, thus achieving stable detection and solving the problem of poor stability.

The present invention relates to the technical field of meat product detection, and discloses a meat product additive multi-parameter rapid detection method, which comprises: a, mixing a sample to be detected with a first detection reagent to obtain a mixed solution, b, the mixed solution is applied to a detection area on a detection test strip, beta-lactam antibiotics in a sample are detected through color changes, and beta-lactamase is fixed to the detection area; and c, applying a second detection reagent to the detection area, and detecting the sulfonamides in the sample through color change of the coupling reaction. The combined detection of beta-lactam antibiotics and sulfonamides is realized by dropwise adding two detection reagents on the same detection area of a single detection test strip step by step, and compared with a technology needing two independent detection methods or two test strips, the combined detection method has the advantages that the use of a detection medium is reduced, the cost is reduced, and the detection efficiency is improved. The operation steps are simplified; the detection cost is reduced; and the detection process is accelerated.

The application belongs to the technical field of biological detection, and particularly relates to a visual detection kit for specifically diagnosing Theileria annulata under constant temperature conditions; the kit comprises RPA reaction products, crRNA, Cas13a protein, RNase inhibitor, reporter molecule, T7 RNA polymerase, NTP and NTT, the RPA reaction products are obtained by amplification of a primer pair shown in SEQ ID NO. 4 and 5, and the sequence of the crRNA is shown in SEQ ID NO. 1; the method is that the RPA reaction products, the crRNA, the Cas13a protein, the RNase inhibitor, the reporter molecule, the T7 RNA polymerase, the NTP and the NTT are mixed and incubated at an isothermal temperature; the incubation product is transferred into a detection buffer, inserted into a test strip, and the result is observed after incubation. The detection method has high sensitivity and high specificity, and can be used for specific detection of Theileria annulata.

The utility model provides a blood glucose detector, which solves the problem that the existing blood glucose detector with a single cavity structure cannot meet the modern daily small and portable requirements and the like, and adopts the main scheme that the blood glucose detector comprises a first shell and a second shell, the top surface of the first shell is concavely provided with a first space, and an integrated special-shaped plate is fixed on one side of the top surface of the first shell; the special-shaped plate comprises at least one flange, the flange is smoothly connected with the outer edge of the top face of the first shell, the outer edge of the bottom face of the second shell and the flange and the outer edge of the flange connected with the top face of the first shell are magnetically attracted and fixed into a whole, the interior of the second shell is hollowed out, and the bottom face of the second shell is sealed through an assembly plate to form a second space. The first space is used for containing a needle head and test paper, a PCB and a display screen electrically connected with the PCB are arranged in the second space, and an insertion opening is formed in one side of the assembly plate so that the test paper can be inserted into the insertion opening to react with the PCB.

The application discloses a photothermal lateral flow analysis device for simultaneously detecting multiple pathogenic bacteria and application thereof. The device comprises a test strip, n photothermal signal probes, an 808nm plane laser light source and a thermal detection device, the n photothermal signal probes are prepared by respectively adsorbing n pathogenic bacteria antibodies, i.e. first primary antibodies, on the surface of CuS@SnS2-Au composite, and n is greater than or equal to 2; the second primary antibodies of the n pathogenic bacteria to be detected are sequentially fixed on the detection area of the test strip, and the second primary antibodies of the n pathogenic bacteria to be detected are sequentially fixed on the detection area of the test strip. The application steps are as follows: the n photothermal signal probes, the sample liquid to be detected and the buffer are mixed, the sample pad end of the test strip is inserted into the mixed solution for chromatography, the whole detection area is irradiated by the 808nm plane laser, and the photothermal temperature of the detection area is collected by a thermal imaging or temperature measuring device; standard curves are established according to the temperature and the concentration of the n pathogenic bacteria to be detected, and photothermal qualitative and quantitative analysis of the n pathogenic bacteria to be detected is carried out.

The invention discloses a primer and a kit for rapidly detecting yersinia rouxii through RPA-CRISPR / Cas12a and application of the primer and the kit, and relates to the technical field of disease prevention and control in aquaculture. The invention aims to solve the problems that the existing detection method is time-consuming and labor-consuming, is easy to cause pollution and needs to be matched with professional detection equipment. According to the present invention, the RPA amplification primer is composed of a forward primer yrp1-RPA-F2 and a reverse primer yrp1-RPA-R2, the crRNA is yrp1-crRNA-R1, and the kit for rapid detection of yersinia rouxii contains the RPA amplification primer and the crRNA. The method for rapidly detecting yersinia rouxii comprises a fluorescence method and a test paper method. The method disclosed by the invention has the advantages of good inclusiveness, strong specificity, high sensitivity and the like, detection is carried out based on the RPA primer and the crRNA, and the obtained RPA product is detected by using a lateral flow chromatography test strip and fluorescence, so that the purpose of portable visual rapid detection on site or in a breeding base is achieved, and the method is suitable for large-scale popularization and application. The method has very high clinical application value in the aspects of early discovery of diseases, epidemiological investigation and comprehensive prevention and control.