A method for improving the quality of pond water used in
aquaculture applications by adding to the pond water active
bacteria that are preferably germinated from spores on site using a
nutrient-germinant composition and an incubation method for increased
spore germination efficiency, in combination with a
nitrification enhancement agent such as
calcium carbonate or calcified seaweed, and an optional reaction surface area modifier such as calcified seaweed or plastic or
metal particles or fragments. The
nutrient-germinant composition comprises L-amino acids, D-glucose and / or D-
fructose, a
phosphate buffer, an industrial
preservative, and may include
bacteria spores (preferably of one or more
Bacillus species) or they may be separately combined for
germination. The incubation method comprises heating a
nutrient germinant composition and
bacteria spores, to a temperature range of 35° C. to 60° C. for around 2 to 60 minutes to produce an incubated bacteria solution that is discharged to the
aquaculture application.