131 results about "Spore germination" patented technology

The invention discloses a microbial agent and application thereof in preventing and treating rice blast and promoting rice growth, active ingredients of the microbial agent are formed by compounding cold bacillus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO: 33492 and bacillus velezensis with the preservation number of CGMCC NO: 33493, and the microbial agent aims at solving the synergistic problem of preventing and treating rice blast and promoting rice growth. According to the complex microbial inoculant, through the synergistic interaction between the two strains, the metabolite of the complex microbial inoculant can completely inhibit the spore germination of magnaporthe oryzae; meanwhile, the fungicide can degrade cellulose, obviously promote the growth of plant height, fresh weight and dry weight of rice, and realize the integration of disease prevention and growth promotion. The invention further discloses a preparation method of the fungicide and an application method of the fungicide for preventing and treating rice blast and promoting growth in a root irrigation mode in the three-leaf stage of rice. The microbial agent disclosed by the invention has dual functions of efficiently preventing diseases and stably promoting growth, and an efficient and safe biological solution is provided for green and sustainable development of agriculture.

Provided is a stabilized aqueous hydrogen peroxide composition comprising a sugar alcohol that does not contain silver, organophosphonate, peroxyacetic acid or other peracid, or stannate. The composition optionally includes a carboxylic acid. The composition is suitable as a disinfectant and oxidizer, and for the treatment, inhibition, or eradication of fungi or nematodes. Also provided is a method for reversing a bacterial or fungal infection in an infected plant, and a method for enhancing plant growth and / or productivity. Also provided is a method for inhibiting or preventing fungal spore germination in soil in a fungal infested area. Also provide is a method of controlling or preventing nematode infestation of a plant, and a method of reversing damage of a plant caused by nematode infestation. Also provided is a method for treating or reversing Panama disease in an infected banana plant.

The invention belongs to the technical field of plant growth-promoting bacteria, and particularly relates to protozoa trichomonad cooperating with bacillus to prevent and control cucumber fusarium wilt and application of the protozoa trichomonad. The invention provides a strain of protozoa trichomonas mtoleri (Cercomonas mtoleri) B5, and the preservation number of the strain is CCTCC (China Center for Type Culture Collection) N0: V202502. The protozoa trichomonad B5 provided by the invention can induce spore germination of a biocontrol strain bacillus velezensis SQR9 and improve the rhizosphere colonization capability of the biocontrol strain SQR9. The protozoa trichomonad B5 and the bacillus velezensis SQR9 are jointly inoculated in cucumber fusarium wilt continuous cropping soil, and the fusarium wilt morbidity of cucumber plants can be effectively reduced.

The invention discloses a hypha mineral composite bionic sand stabilization unit body and a preparation method thereof, and relates to the technical field of sand stabilization, the interior of the unit body is provided with a bionic three-dimensional porous skeleton structure, the unit body comprises a porous core layer, a porous core layer and a porous core layer, the porous core layer takes cleaning sand grains with the particle size of 0.1-2 mm as a sand-based carrier; uniformly mixing and curing with organic nutrient substances, mineral precursors and strain spores; the composite reinforcing layer is formed by interweaving and compounding a mycelium network formed by germination and growth of strain spores, mineral crystals generated by in-situ mineralization induced by metabolic activity of hyphae and a plant fiber reinforcing material which is externally added and embedded into the pores in the pores of the porous core layer; according to the hypha mineral compounded bionic sand stabilization unit body and the preparation method thereof, mold pressing or 3D printing prefabrication is adopted, the structure is controllable, and standardized production and transportation are facilitated; and through a controllable fermentation and gradient dehumidification mineralization culture process, hyphae and minerals are ensured to be uniformly and fully compounded in pores, and the product quality is stable.

The invention relates to a wheat gibberellic disease spore germination form detection method based on microscopic images. Compared with the prior art, the wheat gibberellic disease spore germination form detection method solves the defect that germination form spore microscopic images are difficult to detect. The method comprises the following steps: collecting and preprocessing a wheat scab spore microscopic image; construction of a germination form target key point detection model; training a germination form target key point detection model; acquiring and preprocessing a medicament treatment spore image; and outputting a spore detection result. Through the germination convolution cascade module and the germination recursion gating module, spore sub-pixel level details and a germination tube global contour are accurately captured in a microscopic image, the problem of missing detection and false detection of tiny targets is solved, and microscopic image accurate detection of germinated spores is realized.

The invention relates to an application of carboxylated chitin or carboxylated chitosan in promoting insect-killing fungi to prevent and treat diseases and insect pests. Research finds that carboxylated chitin or carboxylated chitosan can significantly promote spore germination and hypha growth of insecticidal fungi, also can significantly enhance stress resistance of the insecticidal fungi, significantly enhance toxicity of the insecticidal fungi to pests, improve ultraviolet resistance and high temperature resistance of the insecticidal fungi, and improve the insecticidal activity of the insecticidal fungi. The invention also finds that the carboxylated chitin or chitosan can effectively inhibit the growth of Xanthomonas oryzae pv. Oryzae. Therefore, the carboxylated chitin or the carboxylated chitosan can be used for preparing a product for enhancing the insecticidal fungus effect and a product for preventing and treating the rice bacterial leaf blight, and the prevention and treatment of plant diseases and insect pests are realized. In addition, carboxylated chitin and carboxylated chitosan are derived from natural polymer materials, have the characteristics of safety, no toxicity, biodegradability, environmental friendliness and the like, are environment-friendly and economical, and have wide application prospects and market values.

The invention belongs to the technical field of cibotium barometz breeding, and particularly relates to a method for artificially breeding cibotium barometz by using spores, which comprises the following components in parts by weight: 100 parts of field soil, 20-25 parts of sawdust, 10-15 parts of perlite, 10-12 parts of plant ash, 0.05-0.15 part of manganese sulfate and 0.3-0.5 part of sodium selenite; spores germinate, and the environment temperature is 24-26 DEG C; and transplanting the germinated seedlings into a greenhouse for cultivation. According to the method, high spore germination rate and seedling survival rate are ensured, and the transplanted seedlings can grow smoothly.

The embodiment of the invention provides a grain fungal disease early warning method, device and equipment and a computer storage medium. The early warning method for the fungal diseases of the grains comprises the following steps: constructing a fungal germination index model according to a coupling relationship between canopy temperature and leaf surface humidity of the grains; inputting canopy temperature data and leaf surface humidity data of grains into the fungal germination index model to obtain a fungal spore germination probability; and when the fungal spore germination probability meets a threshold condition, generating early warning information of cereal fungal diseases. According to the embodiment of the invention, by constructing the fungus germination index model driven by coupling of the canopy temperature and the leaf surface humidity, the biophysical parameters of the grains are quantified into the fungal spore germination probability, the physiological reactions of fungi in different environments are monitored in real time in the early warning process of the fungal diseases of the grains, and the early warning accuracy of the fungal diseases of the grains is improved.

The invention relates to the technical field of cyanobacterial bloom control in water, and particularly discloses a compound enzyme-bacterium synergistic preparation for cyanobacterial bloom control, a preparation method and a use method. The preparation comprises the following components in percentage by mass: compound enzymes (10-20% of cellulase, 8-12% of beta-1, 3-glucanase and 3-8% of lipase), functional bacteria (10-20% of bacillus subtilis, 8-15% of trichoderma harzianum and 5-10% of bacillus amyloliquefaciens), an enzyme stabilizer (3-8% of trehalose), an adsorption carrier (15-25% of Fe < 3 + > modified charcoal and 8-15% of diatomite) and auxiliary components. The preparation method comprises the steps of carrier modification, solid fermentation and microcapsule embedding. During use, 3-20kg / 1000m < 3 > of water is added for the first time, 2-10kg / 1000m < 3 > of water is added every 7-14 days in the maintenance stage, and synchronous aeration is performed. Through the synergistic effect of beta-1, 3-glucanase and trichoderma harzianum, the algicidal rate is 92%, and the spore germination inhibition rate is 82%; the Fe < 3 + > modified charcoal improves the stability of the enzyme in an alkaline environment (80% of activity is retained when the pH value is 9); the method is suitable for eutrophic water with chlorophyll a greater than or equal to 30 [mu] g / L, and has no chemical residue risk.

The invention belongs to the technical field of agricultural control, and particularly relates to a pesticide for inhibiting fusarium graminearum as well as a preparation method and application thereof. The pesticide is a chitosan / chestnut flower polysaccharide composite material, and the chitosan / chestnut flower polysaccharide composite material is obtained by forming nucleophilic substitution between-COOH in chestnut flower polysaccharide and-NH2 in chitosan and generating amido bonds; the chestnut flower polysaccharide is obtained through a water extraction and alcohol precipitation method. As a pesticide, the chitosan / chestnut flower crude polysaccharide composite material has a good antibacterial rate, EC50 of fusarium graminearum hypha growth and spore germination are 442.6318 mu g / mL and 8.3516 mu g / mL respectively, in addition, the chitosan / chestnut flower crude polysaccharide composite material can promote germination of wheat seeds, the content of photosynthetic pigments in leaves is increased, and the yield of the wheat seeds is increased. And the activity of crop pathogenic bacterium defensive enzyme is improved.

The invention discloses a polysaccharide polymer, application of the polysaccharide polymer and a preparation method of the polysaccharide polymer. The polysaccharide polymer QPS capable of remarkably selectively inhibiting harmful bacteria in aquaculture is separated and purified from the water mold antagonistic actinomycete strain QHV2 for the first time, can effectively inhibit mycelial growth and spore germination of water mold / branchiomycosis and destroy mature biological membranes, is stable in activity, free of toxic effects on fry and good in biological safety, and can be applied to the field of aquaculture. The material can be used as a candidate material for research and development of a fish pathogenic fungus biological prevention and control agent. Meanwhile, the polysaccharide polymer QPS avoids pollution of harmful substances to a water body when a QHV2 fermentation product is directly applied, can selectively inhibit staphylococcus aureus, saprolegnia, branchiomycosis and other harmful bacteria in aquaculture, and has no inhibition or weak inhibition on pseudomonas pseudoalcaligenes, bacillus and other beneficial bacteria in aquaculture. And the application effect is better.

A process 100 for the decontamination and microbiological stabilisation of liquid egg white stored at room temperature, comprising the operating steps of: preparing the egg white in a liquid state; carrying out a first thermal treatment 110 by carrying out a first and a second sub-step for heating to specific temperature ranges and for specific time intervals in order to eliminate part of the microorganisms present in the egg white and activate any spores present; incubating 130 the egg white at a pre-established temperature and for a specific incubation time interval for the germination of any spores present; carrying out a second thermal treatment 140 on the egg white equivalent to the first to eliminate the spore-derived bacteria; carrying out an isothermal treatment 160 of the egg white to eliminate the bacteria present in the egg white that were not eliminated by the second thermal treatment; carrying out a third thermal treatment 170 similar to the aforesaid first and second thermal treatment in order to eliminate the bacteria present in the egg white that were not eliminated by said first and second thermal treatments.

The application provides a strain of antagonistic cucumber downy mildew Microbacterium luteum C10-2 and application thereof, and belongs to the technical field of biocontrol bacteria. The Microbacterium luteum C10-2 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20253025. The strain can effectively inhibit cucumber downy mildew. Indoor test shows that the spore germination inhibition rate of the strain on Pseudoperonospora cubensis is 86.54%, the leaf disc prevention efficiency is 84.23%, and the strain is superior to the chemical agent dimethomorph; the fermentation liquor of the strain is sprayed in a field at a concentration of 1x10 8 cfu / mL, and the prevention efficiency reaches 80.50%. The strain can stably colonize in cucumber, inhibit pathogenic bacteria infection, and is environment-friendly, free of drug hazards and not prone to drug resistance. The application provides a new strain resource for green prevention and control of cucumber downy mildew, and has a wide application prospect.

The invention discloses a Ustilaginoidea virens inoculant, a preparation method and application of the Ustilaginoidea virens inoculant in Ustilaginoidea virens inoculation, and belongs to the technical field of agricultural microorganisms. According to the method, two-step shake-flask culture is combined with the steps of filtering, centrifuging and spore germination, so that the ustilaginoidea virens inoculum taking germinated spores (GC) as the core is prepared, and the problems of hypha aging, low spore activity, asynchronous infection and the like in the existing inoculation method are effectively solved. The inoculant has a tender hypha structure and strong infection activity, and the inoculation efficiency and the pathogenesis consistency are remarkably improved. When being used for preparing transcriptome samples, metabolome samples and other omics samples, the strain avoids non-specific immune interference caused by PAMPs released by broken hyphae, ensures synchronous infection process, and is real and reliable in immune response. The invention provides a stable and efficient inoculation system with a clean background for the research on the ustilaginoidea virens-rice interaction mechanism, and has a good application prospect.

The present invention pertains to use of a composition comprising propionic acid and / or salt thereof and at least one fatty acid having 6 to 12 carbon atoms for reducing dormant spore viability, for inactivating dormant conidia, for eliminating or reducing germination of conidia, for increasing cell damage and / or cell death of hyphae, and / or for reducing or eliminating germ tube formation.

The invention discloses paenibacillus FHY1 and application thereof, and belongs to the field of microorganisms. A strain of paenibacillus FHY1 is screened from Xinjiang populus euphratica juice, and experimental verification shows that a liquid culture of the paenibacillus FHY1 strain provided by the invention has a remarkable inhibition effect on magnaporthe oryzae, the bacteriostasis rate can reach 84%, and the inhibition rate on magnaporthe oryzae spore germination is 92%. And the rice growth can be promoted under the conditions of 150mM NaCl and 250mM NaCl. Meanwhile, the strain also has a good inhibition effect on pyricularia grisea, fusarium oxysporum, fusarium graminearum, sclerotinia sclerotiorum and colletotrichum gloeosporioides. Therefore, the invention provides a new strain with a good control effect on pathogenic fungi, and meanwhile, the growth and development of rice are remarkably promoted in a salt stress environment.

The present application provides a novel structure of thiophene compound, the compound has significant inhibitory effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as Pyricularia oryzae, Colletotrichum gloeosporioides, Gibberella, Phytophthora, Phytophthora capsici), which can be used for preventing and treating plant diseases caused by fungi and / or oomycetes.

The invention discloses preparation and application of a double-component dsRNA (double-stranded ribonucleic acid) medicament for enhancing resistance mutation risk capability of RNA (ribonucleic acid) pesticide to botrytis cinerea, and a key pathogenic gene of botrytis cinerea is any one or two of a botrytis cinerea global regulatory factor Vel1 gene or a galacturonic acid reductase Gar2 gene. The dsRNA is any one or two of dsRNAs targeting a Vel1 gene or a Gar2 gene, and efficient prevention and control of gray mold are achieved through key pathogenic links such as spore germination inhibition, hypha colonization and infection structure formation. In addition, the effectiveness of the RNA pesticide aiming at botrytis cinerea after single target gene mutation is evaluated through cooperative use of the combination of the double-component RNA, the ds-Gar2 and the ds-Vel1, the good effect of the double-target synergistic effect in the aspect of reducing the off-target risk is proved, and the practice and popularization of a green prevention and control technology in prevention and control of gray mold are promoted.

The present application provides a kind of streptomyces, named Streptomyces sp., is registered and preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No:65917.The present application also provides fermentation broth of the streptomyces and ethanol extract and crude extract of fermentation broth thereof.The new species of streptomyces and its crude extract and the like of the present application have broad-spectrum antibacterial activity, have good antagonism to many pathogenic bacteria such as banana long-shaped spot fungus and banana anthracnose fungus, and can make banana anthracnose fungus hyphae wrinkle, deform, break, inhibit banana anthracnose fungus spore germination, make banana anthracnose fungus spore germination wrinkle, break, inhibit banana anthracnose fungus growth, and can also improve peroxidase, polyperoxidase, CAT activity and the like in banana peel, is a potential biological agent for preventing and treating anthracnose disease, stem rot disease and the like, has broad development space, and has good development and application prospect.

The utility model relates to the technical field of pest control, in particular to a spore release storage container which comprises a barrel and an electric control air valve, a spore outlet is formed in the peripheral side wall of the barrel, the electric control air valve is detachably assembled at the spore outlet, and openings are formed in the two ends of the barrel. The opening is used for adding spores into the cylinder tank or removing spores in the cylinder tank, a first sealing cover and a second sealing cover are detachably assembled at the two ends of the cylinder tank respectively, an elastic vibration plate is arranged in the first sealing cover in the cross section direction of the first sealing cover, and the elastic vibration plate is used for making contact with an external vibration source; and the elastic vibration plate which vibrates at high frequency is used for changing the size of the internal space of the cylinder so as to intermittently extrude spores from the electric control air valve. According to the storage container for releasing the spores, the electric control air valve is closed to prevent moisture in external air from entering the cylinder tank, and germination of the spores in the medicine powder barrel is not prone to being caused, so that intrusive activity of the spores is effectively prevented from being lost, and the disease and pest control effect of the spores is obviously improved.

The invention provides a penicillium oxalicum strain K3R2 and a preparation method and application of a biological agent of the penicillium oxalicum strain K3R2. The biocontrol bacterium Penicillium oxalicum strain K3R2 screened by the invention is preserved in the China General Microbiological Culture Collection Center on September 25, 2025, the preservation number is CGMCC NO.42219, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing; the ITS sequence of the Penicillium oxalicum strain K3R2 is as shown in SEQ ID NO: 1, and the CaM sequence is as shown in SEQ ID NO: 2. The penicillium oxalicum strain K3R2 provided by the invention can inhibit hypha growth and spore germination of beet neocam spores, the hypha inhibition rate is 83.60%, the spore inhibition rate is 65.31%, and the penicillium oxalicum strain K3R2 has the effect of preventing and treating quinoa stem blight. Meanwhile, the penicillium oxalicum strain K3R2 has the capabilities of salt resistance, alkali resistance, phosphate solubilization, potassium solubilization, cellulose solubilization and nitrogen fixation; the strain K3R2 remarkably promotes the growth of quinoa plants, so that the chlorophyll content is increased by 20.22%, and the accumulation of nutrient substances is accelerated; the fresh weight, the dry weight and the stem diameter of quinoa plants are increased, and the lodging resistance is enhanced.

An antimicrobial peptide against Bacillus cereus, a preparation method therefor and a use thereof. The antimicrobial peptide against bacillus cereus has the general amino acid sequence formula of [KR]GG[KR]XC[FWY]C[KR]X[KR][FWY]C[LIAV]CXG[KR][KR], wherein the antimicrobial peptide XWC26 has a strong antibacterial activity against Bacillus cereus, efficiently inhibiting the spore germination of Bacillus cereus, and effectively controlling Bacillus cereus contamination in food. The antimicrobial peptide against bacillus cereus has the amino acid sequence of KGGKLCWCKNKWCICLGKR, with a molecular weight of 2224.79 Da, an isoelectric point of 9.70, and a net charge of 6. The antimicrobial peptide also has the advantages of high stability, reduced risk of drug resistance induction, high synthesis efficiency, low production costs, etc.

This invention belongs to the field of agricultural biotechnology, specifically relating to a strain of Pseudomonas sp. R10 and its applications. The Pseudomonas sp. R10 provided by this invention is deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC M 2024850. This strain exhibits significant control effects against clubroot disease, particularly in rapeseed. Verification through examples shows that Pseudomonas sp. R10 inhibits the germination rate of dormant spores of Pseudomonas by 35.14%. In pot experiments, it reduced the clubroot disease severity index by 53.06%. In field trials, treatment of rapeseed with R10 solution reduced the clubroot disease severity index by 39.45%, and significantly increased plant height, rootstock diameter, fresh weight, number of effective branches, and number of effective siliques in the main inflorescence compared to the control plot. R10 solution treatment also increased rapeseed yield by 59.86% and thousand-grain weight by 7.25%.

The present application provides a kind of compound composition for preventing and treating plant wilt, the composition composition includes 4-hydroxy cinnamic acid and thymol, mass ratio is 1:1~1:2;The mass ratio of the composition is 1:1, the concentration of the composition is 60~300 μg / mL;The mass ratio of the composition is 1:2, the concentration of the composition is 50~200 μg / mL;The present application can inhibit the growth of pepper fusarium oxysporum f.sp.cubense mycelium, spore germination and sporulation, and can play a synergistic effect.The composition in the present application can significantly reduce the incidence of wilt in pot experiment.Compared with the prior art, the present application aims to reduce the use of chemical pesticides by low-cost, safe and efficient plant-derived antibacterial compounds.

The invention provides a method for improving the efficiency of infecting host larvae by cordyceps sinensis, and belongs to the technical field of artificial cultivation of cordyceps sinensis. The method comprises the following steps: inoculating cordyceps sinensis to a cordyceps sinensis liquid culture medium containing hepialus armoricanus larva ecdysis shell grinding liquid, and culturing to obtain cordyceps sinensis liquid; and smearing the cordyceps sinensis bacterial liquid on the epidermis of the host larva for infection. The cordyceps sinensis is cultured by adopting the hepialus larva ecdysis shell grinding fluid, on one hand, a nutrition source can be provided for growth of the cordyceps sinensis, the spore germination rate is remarkably increased, and infectious microbe pollution is reduced; on the other hand, host recognition receptors (glycoprotein) on the surfaces of spores can be activated, the adhesion capacity and infection efficiency of cordyceps sinensis on the body walls of larvae are enhanced, and the rigidification time is shortened.

The invention discloses an antibacterial peptide, a preparation method and application thereof and an antibacterial drug, and relates to the technical field of antibacterial peptides, the amino acid sequence of the antibacterial peptide is NH2-RRWRIVLIRARK-CONH2, and the preparation method is a polypeptide solid-phase synthesis method. The antibacterial peptide is wide in acid-base stability range from pH 2 to pH 9; the temperature stability range is wide and ranges from-20 DEG C to 50 DEG C; 95% ethanol resistance is realized; the shelf life is stable and exceeds 6 months. According to the antibacterial peptide provided by the invention, the trichophyton mentagrophytes are killed by inhibiting spore germination of the trichophyton mentagrophytes and causing hypha breakage, the trichophyton mentagrophytes do not generate drug resistance to the antibacterial peptide, the 1% concentration of the antibacterial peptide has an obvious treatment effect on a guinea pig model infected by the trichophyton mentagrophytes, and the antibacterial peptide has a good application prospect as a novel candidate drug for treating trichophyton mentagrophytes infection.

The application discloses a kind of bat moth larvae double bacteria synergistic infection methods, belong to microbial engineering and medicinal fungus cultivation technical field, comprising: 1-2 age larvae are preprocessed by containing first probiotic feed feeding, optimize intestinal microenvironment;3-4 age larvae are injected and infected using mixed bacterial liquid containing second probiotic and Chinese hairy hair fungus, promote spore germination and colonization;5 age and above larvae are stably cultivated by third probiotic spraying, whole process is matched with dynamic monitoring and threshold emergency control.The technology adopts age differentiation operation of low-age feeding-middle-age accurate injection-high-age spraying, matches each stage functional targeted probiotic, constructs suitable mycelium germination microenvironment by means of probiotic metabolite, inhibits miscellaneous bacteria pollution and provides adenosine synthesis precursor, realizes the excellent effect of infection rate improvement, mortality reduction, adenosine content improvement and effective content improvement, provides efficient feasible technical path for large-scale high-quality cultivation of cordyceps sinensis.

The present application relates to the field of microbial technology, and discloses a spore-producing Metarhizium anisopliae strain and its application in the prevention and treatment of western flower thrips. The Metarhizium anisopliae provided by the present application has a preservation number of CCTCC No. M 20252118, a fast growth rate, a large spore yield, a high spore germination rate, and is easy to be cultured and multiplied in large quantities indoors. The present application can be used as a biocontrol fungus for preventing and treating the invasive agricultural pest western flower thrips, has strong pathogenicity to the western flower thrips nymphs, is safe to the environment, is not easy to make the pests resistant, and has application value.

The present invention relates to the field of plant tissue culture technology, and in particular to a method for cultivating Alsophila spinulosa. The method comprises the following steps: inoculating a disinfected spore suspension into a culture medium for cultivation; transferring germinated spore prothallus to an induction GGB culture medium for cultivation; cutting the GGB into small pieces and transferring them to a proliferation culture medium for cultivation; inducing sporophytes and rooting using an incomplete tissue culture method to obtain rooted seedlings; transferring the rooted seedlings to an outdoor greenhouse for cultivation to obtain tissue culture seedlings, and transplanting the tissue culture seedlings onto a mixed substrate to obtain Alsophila spinulosa seedlings. The method provided by the present invention has the characteristics of a high spore germination induction rate, a large GGB proliferation multiple, a high sporophyte induction rate, a high rooting rate, a well-developed root system, sturdy rooted seedlings, a high transplant survival rate, robust seedling growth, and low seedling cultivation costs, thereby providing an efficient and stable tissue culture and rapid propagation technology system for Alsophila spinulosa using the GGB pathway.

The invention discloses a double-stranded RNA-nano material composite carrier and application thereof in prevention and treatment of soybean rust. A fungus extracellular membrane protein PpAFF gene of the soybean rust pathogen is taken as a target, a transcript of the fungus extracellular membrane protein PpAFF gene is taken as a template to synthesize double-stranded RNA, and the double-stranded RNA is sprayed on the surfaces of soybean leaves, so that the PpAFF gene of the soybean rust pathogen can be silenced, and the symptom of the soybean rust disease can be effectively relieved. The base sequence of the gene is as shown in SEQ ID NO: 1. The synthesized nano material MNB can inhibit germination of soybean rust uredospores, stimulate immunoreactions of various plants such as soybeans, wheat, rice and hot peppers, and improve the resistance of crops to pathogenic bacteria. The prevention and control method of spraying the MNB-dsPpAFF compound on the plant surface is green and safe, the prevention and control effect is remarkable, and a very potential solution is provided for prevention and control of important crop fungal diseases such as soybean rust and rice blast.