85 results about "Spore germination" patented technology

The invention discloses a microbial agent and application thereof in preventing and treating rice blast and promoting rice growth, active ingredients of the microbial agent are formed by compounding cold bacillus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO: 33492 and bacillus velezensis with the preservation number of CGMCC NO: 33493, and the microbial agent aims at solving the synergistic problem of preventing and treating rice blast and promoting rice growth. According to the complex microbial inoculant, through the synergistic interaction between the two strains, the metabolite of the complex microbial inoculant can completely inhibit the spore germination of magnaporthe oryzae; meanwhile, the fungicide can degrade cellulose, obviously promote the growth of plant height, fresh weight and dry weight of rice, and realize the integration of disease prevention and growth promotion. The invention further discloses a preparation method of the fungicide and an application method of the fungicide for preventing and treating rice blast and promoting growth in a root irrigation mode in the three-leaf stage of rice. The microbial agent disclosed by the invention has dual functions of efficiently preventing diseases and stably promoting growth, and an efficient and safe biological solution is provided for green and sustainable development of agriculture.

The invention discloses a hypha mineral composite bionic sand stabilization unit body and a preparation method thereof, and relates to the technical field of sand stabilization, the interior of the unit body is provided with a bionic three-dimensional porous skeleton structure, the unit body comprises a porous core layer, a porous core layer and a porous core layer, the porous core layer takes cleaning sand grains with the particle size of 0.1-2 mm as a sand-based carrier; uniformly mixing and curing with organic nutrient substances, mineral precursors and strain spores; the composite reinforcing layer is formed by interweaving and compounding a mycelium network formed by germination and growth of strain spores, mineral crystals generated by in-situ mineralization induced by metabolic activity of hyphae and a plant fiber reinforcing material which is externally added and embedded into the pores in the pores of the porous core layer; according to the hypha mineral compounded bionic sand stabilization unit body and the preparation method thereof, mold pressing or 3D printing prefabrication is adopted, the structure is controllable, and standardized production and transportation are facilitated; and through a controllable fermentation and gradient dehumidification mineralization culture process, hyphae and minerals are ensured to be uniformly and fully compounded in pores, and the product quality is stable.

The embodiment of the invention provides a grain fungal disease early warning method, device and equipment and a computer storage medium. The early warning method for the fungal diseases of the grains comprises the following steps: constructing a fungal germination index model according to a coupling relationship between canopy temperature and leaf surface humidity of the grains; inputting canopy temperature data and leaf surface humidity data of grains into the fungal germination index model to obtain a fungal spore germination probability; and when the fungal spore germination probability meets a threshold condition, generating early warning information of cereal fungal diseases. According to the embodiment of the invention, by constructing the fungus germination index model driven by coupling of the canopy temperature and the leaf surface humidity, the biophysical parameters of the grains are quantified into the fungal spore germination probability, the physiological reactions of fungi in different environments are monitored in real time in the early warning process of the fungal diseases of the grains, and the early warning accuracy of the fungal diseases of the grains is improved.

A process 100 for the decontamination and microbiological stabilisation of liquid egg white stored at room temperature, comprising the operating steps of: preparing the egg white in a liquid state; carrying out a first thermal treatment 110 by carrying out a first and a second sub-step for heating to specific temperature ranges and for specific time intervals in order to eliminate part of the microorganisms present in the egg white and activate any spores present; incubating 130 the egg white at a pre-established temperature and for a specific incubation time interval for the germination of any spores present; carrying out a second thermal treatment 140 on the egg white equivalent to the first to eliminate the spore-derived bacteria; carrying out an isothermal treatment 160 of the egg white to eliminate the bacteria present in the egg white that were not eliminated by the second thermal treatment; carrying out a third thermal treatment 170 similar to the aforesaid first and second thermal treatment in order to eliminate the bacteria present in the egg white that were not eliminated by said first and second thermal treatments.

The application provides a strain of antagonistic cucumber downy mildew Microbacterium luteum C10-2 and application thereof, and belongs to the technical field of biocontrol bacteria. The Microbacterium luteum C10-2 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20253025. The strain can effectively inhibit cucumber downy mildew. Indoor test shows that the spore germination inhibition rate of the strain on Pseudoperonospora cubensis is 86.54%, the leaf disc prevention efficiency is 84.23%, and the strain is superior to the chemical agent dimethomorph; the fermentation liquor of the strain is sprayed in a field at a concentration of 1x10 8 cfu / mL, and the prevention efficiency reaches 80.50%. The strain can stably colonize in cucumber, inhibit pathogenic bacteria infection, and is environment-friendly, free of drug hazards and not prone to drug resistance. The application provides a new strain resource for green prevention and control of cucumber downy mildew, and has a wide application prospect.

The invention discloses a Ustilaginoidea virens inoculant, a preparation method and application of the Ustilaginoidea virens inoculant in Ustilaginoidea virens inoculation, and belongs to the technical field of agricultural microorganisms. According to the method, two-step shake-flask culture is combined with the steps of filtering, centrifuging and spore germination, so that the ustilaginoidea virens inoculum taking germinated spores (GC) as the core is prepared, and the problems of hypha aging, low spore activity, asynchronous infection and the like in the existing inoculation method are effectively solved. The inoculant has a tender hypha structure and strong infection activity, and the inoculation efficiency and the pathogenesis consistency are remarkably improved. When being used for preparing transcriptome samples, metabolome samples and other omics samples, the strain avoids non-specific immune interference caused by PAMPs released by broken hyphae, ensures synchronous infection process, and is real and reliable in immune response. The invention provides a stable and efficient inoculation system with a clean background for the research on the ustilaginoidea virens-rice interaction mechanism, and has a good application prospect.

The invention discloses paenibacillus FHY1 and application thereof, and belongs to the field of microorganisms. A strain of paenibacillus FHY1 is screened from Xinjiang populus euphratica juice, and experimental verification shows that a liquid culture of the paenibacillus FHY1 strain provided by the invention has a remarkable inhibition effect on magnaporthe oryzae, the bacteriostasis rate can reach 84%, and the inhibition rate on magnaporthe oryzae spore germination is 92%. And the rice growth can be promoted under the conditions of 150mM NaCl and 250mM NaCl. Meanwhile, the strain also has a good inhibition effect on pyricularia grisea, fusarium oxysporum, fusarium graminearum, sclerotinia sclerotiorum and colletotrichum gloeosporioides. Therefore, the invention provides a new strain with a good control effect on pathogenic fungi, and meanwhile, the growth and development of rice are remarkably promoted in a salt stress environment.

The present application provides a novel structure of thiophene compound, the compound has significant inhibitory effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as Pyricularia oryzae, Colletotrichum gloeosporioides, Gibberella, Phytophthora, Phytophthora capsici), which can be used for preventing and treating plant diseases caused by fungi and / or oomycetes.

The present application provides a kind of streptomyces, named Streptomyces sp., is registered and preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No:65917.The present application also provides fermentation broth of the streptomyces and ethanol extract and crude extract of fermentation broth thereof.The new species of streptomyces and its crude extract and the like of the present application have broad-spectrum antibacterial activity, have good antagonism to many pathogenic bacteria such as banana long-shaped spot fungus and banana anthracnose fungus, and can make banana anthracnose fungus hyphae wrinkle, deform, break, inhibit banana anthracnose fungus spore germination, make banana anthracnose fungus spore germination wrinkle, break, inhibit banana anthracnose fungus growth, and can also improve peroxidase, polyperoxidase, CAT activity and the like in banana peel, is a potential biological agent for preventing and treating anthracnose disease, stem rot disease and the like, has broad development space, and has good development and application prospect.

The invention provides a penicillium oxalicum strain K3R2 and a preparation method and application of a biological agent of the penicillium oxalicum strain K3R2. The biocontrol bacterium Penicillium oxalicum strain K3R2 screened by the invention is preserved in the China General Microbiological Culture Collection Center on September 25, 2025, the preservation number is CGMCC NO.42219, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing; the ITS sequence of the Penicillium oxalicum strain K3R2 is as shown in SEQ ID NO: 1, and the CaM sequence is as shown in SEQ ID NO: 2. The penicillium oxalicum strain K3R2 provided by the invention can inhibit hypha growth and spore germination of beet neocam spores, the hypha inhibition rate is 83.60%, the spore inhibition rate is 65.31%, and the penicillium oxalicum strain K3R2 has the effect of preventing and treating quinoa stem blight. Meanwhile, the penicillium oxalicum strain K3R2 has the capabilities of salt resistance, alkali resistance, phosphate solubilization, potassium solubilization, cellulose solubilization and nitrogen fixation; the strain K3R2 remarkably promotes the growth of quinoa plants, so that the chlorophyll content is increased by 20.22%, and the accumulation of nutrient substances is accelerated; the fresh weight, the dry weight and the stem diameter of quinoa plants are increased, and the lodging resistance is enhanced.

An antimicrobial peptide against Bacillus cereus, a preparation method therefor and a use thereof. The antimicrobial peptide against bacillus cereus has the general amino acid sequence formula of [KR]GG[KR]XC[FWY]C[KR]X[KR][FWY]C[LIAV]CXG[KR][KR], wherein the antimicrobial peptide XWC26 has a strong antibacterial activity against Bacillus cereus, efficiently inhibiting the spore germination of Bacillus cereus, and effectively controlling Bacillus cereus contamination in food. The antimicrobial peptide against bacillus cereus has the amino acid sequence of KGGKLCWCKNKWCICLGKR, with a molecular weight of 2224.79 Da, an isoelectric point of 9.70, and a net charge of 6. The antimicrobial peptide also has the advantages of high stability, reduced risk of drug resistance induction, high synthesis efficiency, low production costs, etc.

The present application provides a kind of compound composition for preventing and treating plant wilt, the composition composition includes 4-hydroxy cinnamic acid and thymol, mass ratio is 1:1~1:2;The mass ratio of the composition is 1:1, the concentration of the composition is 60~300 μg / mL;The mass ratio of the composition is 1:2, the concentration of the composition is 50~200 μg / mL;The present application can inhibit the growth of pepper fusarium oxysporum f.sp.cubense mycelium, spore germination and sporulation, and can play a synergistic effect.The composition in the present application can significantly reduce the incidence of wilt in pot experiment.Compared with the prior art, the present application aims to reduce the use of chemical pesticides by low-cost, safe and efficient plant-derived antibacterial compounds.

The invention provides a method for improving the efficiency of infecting host larvae by cordyceps sinensis, and belongs to the technical field of artificial cultivation of cordyceps sinensis. The method comprises the following steps: inoculating cordyceps sinensis to a cordyceps sinensis liquid culture medium containing hepialus armoricanus larva ecdysis shell grinding liquid, and culturing to obtain cordyceps sinensis liquid; and smearing the cordyceps sinensis bacterial liquid on the epidermis of the host larva for infection. The cordyceps sinensis is cultured by adopting the hepialus larva ecdysis shell grinding fluid, on one hand, a nutrition source can be provided for growth of the cordyceps sinensis, the spore germination rate is remarkably increased, and infectious microbe pollution is reduced; on the other hand, host recognition receptors (glycoprotein) on the surfaces of spores can be activated, the adhesion capacity and infection efficiency of cordyceps sinensis on the body walls of larvae are enhanced, and the rigidification time is shortened.

The application discloses a kind of bat moth larvae double bacteria synergistic infection methods, belong to microbial engineering and medicinal fungus cultivation technical field, comprising: 1-2 age larvae are preprocessed by containing first probiotic feed feeding, optimize intestinal microenvironment;3-4 age larvae are injected and infected using mixed bacterial liquid containing second probiotic and Chinese hairy hair fungus, promote spore germination and colonization;5 age and above larvae are stably cultivated by third probiotic spraying, whole process is matched with dynamic monitoring and threshold emergency control.The technology adopts age differentiation operation of low-age feeding-middle-age accurate injection-high-age spraying, matches each stage functional targeted probiotic, constructs suitable mycelium germination microenvironment by means of probiotic metabolite, inhibits miscellaneous bacteria pollution and provides adenosine synthesis precursor, realizes the excellent effect of infection rate improvement, mortality reduction, adenosine content improvement and effective content improvement, provides efficient feasible technical path for large-scale high-quality cultivation of cordyceps sinensis.

The present application relates to the field of microbial technology, and discloses a spore-producing Metarhizium anisopliae strain and its application in the prevention and treatment of western flower thrips. The Metarhizium anisopliae provided by the present application has a preservation number of CCTCC No. M 20252118, a fast growth rate, a large spore yield, a high spore germination rate, and is easy to be cultured and multiplied in large quantities indoors. The present application can be used as a biocontrol fungus for preventing and treating the invasive agricultural pest western flower thrips, has strong pathogenicity to the western flower thrips nymphs, is safe to the environment, is not easy to make the pests resistant, and has application value.

The invention discloses a double-stranded RNA-nano material composite carrier and application thereof in prevention and treatment of soybean rust. A fungus extracellular membrane protein PpAFF gene of the soybean rust pathogen is taken as a target, a transcript of the fungus extracellular membrane protein PpAFF gene is taken as a template to synthesize double-stranded RNA, and the double-stranded RNA is sprayed on the surfaces of soybean leaves, so that the PpAFF gene of the soybean rust pathogen can be silenced, and the symptom of the soybean rust disease can be effectively relieved. The base sequence of the gene is as shown in SEQ ID NO: 1. The synthesized nano material MNB can inhibit germination of soybean rust uredospores, stimulate immunoreactions of various plants such as soybeans, wheat, rice and hot peppers, and improve the resistance of crops to pathogenic bacteria. The prevention and control method of spraying the MNB-dsPpAFF compound on the plant surface is green and safe, the prevention and control effect is remarkable, and a very potential solution is provided for prevention and control of important crop fungal diseases such as soybean rust and rice blast.

The invention belongs to the technical field of plant disease prevention and treatment, and particularly relates to a polypeptide PP alpha for preventing and treating fungal diseases and application thereof. The invention discloses application of polypeptide PP alpha in inhibiting plant pathogenic fungi and preventing and treating plant diseases caused by the plant pathogenic fungi, the polypeptide PP alpha is a yeast alpha mating factor, and the amino acid sequence of the polypeptide PP alpha is shown as SEQ ID No.1. Experiments prove that the polypeptide PP alpha can significantly inhibit spore germination and appressorium formation of various plant pathogenic fungi such as pyricularia oryzae, cucurbitaceae colletotrichum spinosus, colletotrichum cryptum and botrytis cinerea, so that the infection process of the fungi is blocked. The polypeptide PP alpha provided by the invention has broad-spectrum antifungal activity, can be used as a novel green bactericide for preventing and treating fungal diseases of crops, and has a wide application prospect.

The application discloses a Talaromyces purpureofaciens strain and a fermentation supernatant thereof and application. T. purpureogenus F-4, which has a preservation number of CCTCC NO: M 20252023, has the effects of promoting growth by dephosphorization, nitrogen fixation and iron carrier production, and the effects of preventing and treating diseases such as garlic root rot, potato scab and wheat foot rot, in addition to the effects of resisting salt stress, alkali stress, salt-alkali combined stress, heavy metal stress and osmotic pressure stress. The fermentation supernatant of the Talaromyces purpureofaciens F-4 can inhibit the growth of sporodochia of Fusarium solani, Streptomyces bottropensis and Pseudofusarium pseudotsugae and the spore germination thereof. The Talaromyces purpureofaciens and the fermentation supernatant thereof can effectively control the growth and reproduction of plant pathogenic fungi, reduce the risk of disease occurrence, reduce the dependence on chemical pesticides, reduce environmental pollution and the risk of agricultural product residues, and have important significance for promoting the sustainable development of agriculture.

The invention relates to the field of insect prevention and control, in particular to a high-toxicity metarhizium anisopliae strain IPPMA362 resistant to high temperature and ultraviolet stress and application thereof. The invention aims to screen excellent metarhizium anisopliae strains with high toxicity to bemisia tabaci. Through indoor biological activity determination, the lethal ability of the target strain on the bemisia tabaci nymphs is determined; further, key biological characteristics such as sporulation capability, spore germination rate and ultraviolet resistance of the strain are systematically evaluated, so that the application potential of the strain is judged; and the optimized strain is accurately identified through a molecular biology means, so that a foundation is laid for subsequent strain registration, patent protection and product development.

The invention relates to the technical field of plant disease prevention and treatment, in particular to application of a Welan gum aqueous solution to prevention and treatment of plant diseases, and the concentration of the Welan gum aqueous solution is 50 micrograms per milliliter to 100 micrograms per milliliter. The molecular weight of the welan gum is 800 Da to 900 Da. Experiments prove that the Welan gum aqueous solution can inhibit spore germination and mycelial growth of the original fungus curvularia lunata of the corn curvularia leaf spot, the physiological function of leaves is not affected after spraying, in addition, the Welan gum aqueous solution also has an inhibiting effect on diseases such as northern corn leaf blight and wheat scab, and is expected to be used as a green and safe pesticide, and the Welan gum aqueous solution has broad application prospects. The compound is applied to comprehensive prevention and control of diseases of corn and other crops, and can also be used as a dispersing agent and a slow-release agent to be mixed with other bacteriostatic agents to improve the prevention and control effect.

Oxadiazole antibiotics that exhibit bactericidal activity against C. difficile vegetative cells. We screened a library of 75 oxadiazoles against C. difficile ATCC 43255. The findings from this collection served as the basis for the syntheses of an additional 58 analogs, which were tested against the same strain. We discovered a potent (MIC50=0.5 μg / mL, MIC90=1 μg / mL for 101 C. difficile strains) and narrow-spectrum oxadiazole (3-(4-(cyclopentyloxy)phenyl)-5-(4-nitro-1H-imidazol-2-yl)-1,2,4-oxadiazole; compound 57) that is not active against common gut bacteria or other tested organisms, but is selectively bactericidal against C. difficile and targets cell-wall synthesis. Other similarly effective oxadiazole antibiotics of formula I and II are described herein, several of which inhibit or prevent C. difficile spore germination.

A coating composition that includes a mixture of a water-based coating polymer, an aqueous solution having silver-modified ceria nanoparticles (AgCNPs) and a weight percent loading less than about 3% weight / volume of the mixture, and a spore germination agent having a 0.1-1% weight / volume and the mixture configured for coating a surface.

This invention relates to a method for the germination of Cibotium barometz spores and a method for the propagation of Cibotium barometz, belonging to the field of plant culture technology. The propagation method of this invention includes the following steps: disinfection: disinfecting Cibotium barometz spores with a 10% sodium hypochlorite solution for 5-10 minutes; spore germination: the germination medium used includes 1 / 4 MS medium, 4.5-7 g·L⁻¹. ‑1 Agar and 0-30 g·L ‑1 Sucrose; Sporophyte differentiation: The sporophyte differentiation media used included 1 / 2 MS medium and 0-1 mg / L... ‑1 6-BA, 0.5-1 mg·L ‑1 2,4-D, 4.5-7 g·L ‑1 Agar and 25-30 g·L ‑1 Sucrose; Sporophyte strengthening: The sporophyte strengthening medium used included 1 / 4 MS medium, 4.5–7 g·L⁻¹. ‑1 Agar, 30 g·L ‑1 Sucrose and 1-2 g·L ‑1 Activated carbon; hardening and transplanting: After the tissue culture seedlings are sealed, the seal is gradually lifted, and they are then transferred to a specific mixed substrate for cultivation to obtain commercial seedlings. This method can improve the spore germination rate by using a specific sterilization procedure, and achieves the effect of producing more and better seedlings by using specific culture media and hardening preparations at each stage.

The invention provides application of a small peptide signal molecule to improvement of plant disease resistance. The small peptide signal molecule has an amino acid sequence as shown in SEQ ID NO: 3. The small peptide signal molecule can significantly inhibit spore germination and bud tube growth of pathogenic bacteria-fusarium graminearum causing gibberellic disease. The disease resistance of plants can be remarkably improved by externally applying small peptide signal molecules. The application of the small peptide signal molecule in providing the plant disease resistance has far-reaching significance for improving the seed industry and agricultural technology level and market competitiveness in China.

The invention provides application of a compound HLF1-11 in preparation of a medicine for preventing and controlling magnaporthe oryzae and a medicine for inhibiting spore germination of the magnaporthe oryzae, and further provides a pesticide and a method for preventing and controlling the magnaporthe oryzae. The compound HLF1-11 has an obvious inhibiting effect on development of magnaporthe oryzae appressorium, can effectively inhibit pathogenicity of magnaporthe oryzae spores and can prevent and treat magnaporthe oryzae, so that a foundation is laid for research and development of related prevention and control drugs.

The invention discloses a method for inhibiting germination of clostridium spores and application, and belongs to the field of food microorganisms. The method for inhibiting germination of the clostridium spores comprises the step of adding beet red into a spore-containing system so as to inhibit germination of the spores. The beet red used in the invention is a natural colorant, and has the characteristics of no toxicity and no carcinogenicity; the method does not need to be compounded with other spore germination inhibiting agents for use, and is wide in use temperature range, single in component, convenient to operate and suitable for large-scale popularization and application.

The invention relates to the field of insect prevention and control, in particular to a high-stress-resistance beauveria bassiana strain CIPPBb075 with high pathogenicity to grassland caterpillars and application of the high-stress-resistance beauveria bassiana strain CIPPBb075. According to the invention, diseased insects parasitized by beauveria bassiana are collected from a field, and the strain is separated and purified, so that the CIPPBb075 strain has relatively high toxicity to grassland caterpillar; the germination rate is increased along with temperature rise, and the spore germination rate is obviously higher than that of other strains; the ultraviolet resistance at 25 DEG C is high; the extracellular protease activity is high; the activity of extracellular chitinase is high. Therefore, the CIPPBb075 strain has relatively strong stress resistance, and can be used as an effective microbial agent for preventing and treating the larvae of the grassland caterpillar compared with other strains.

This invention discloses a strain of Bacillus subtilis FHY1 and its applications, belonging to the field of microbiology. The present invention obtained a strain of Bacillus subtilis FHY1 from the juice of Populus euphratica in Xinjiang. Experimental verification shows that the liquid culture of the Bacillus subtilis FHY1 strain provided by this invention has a significant inhibitory effect on rice blast fungus, with an inhibition rate of 84% and an inhibition rate of 92% on rice blast fungus spore germination. It can promote rice growth under conditions of 150mM NaCl and 250mM NaCl. Simultaneously, this strain also has good inhibitory effects on rice blast fungus, Fusarium oxysporum, Fusarium graminearum, Sclerotinia sclerotiorum, and Anthracnose fungus. Therefore, this invention provides a new strain with good control effect against pathogenic fungi, and significantly promotes rice growth and development under salt stress.

The application discloses application of smlt0134 protein in inhibiting rice Magnaporthe oryzae. Experiments prove that the smlt0134 protein can inhibit the rice Magnaporthe oryzae, mainly embodied in inhibiting growth of the rice Magnaporthe oryzae, inhibiting spore germination of the rice Magnaporthe oryzae and / or inhibiting spore growth of the rice Magnaporthe oryzae. Therefore, the smlt0134 protein is expected to develop into a new type of high-efficiency protein preparation. The application has important application value.

This invention discloses a strain of *Bacillus belyssus* S2 and its application in the control of early blight in tomato, belonging to the field of microbial technology. *Bacillus belyssus* S2 is deposited at the China Center for Type Culture Collection (CCTCC) on March 30, 2026, with accession number CCTCC NO: M 2026551. The *Bacillus belyssus* S2 isolated and screened in this invention can secrete siderophores, cellulases, proteases, 1,2-β-glucanases, and lipases. Its soluble and volatile substances can inhibit the mycelial growth of tomato pathogens and cause mycelial malformation, reduce spore germination rate, delay the onset of early blight in tomato fruits, and enhance the fruit's resistance, providing a new microbial resource for the control of diseases such as early blight in tomato.