164 results about "Spore germination" patented technology

The invention provides a streptomyces, which is named as Streptomyces sp. And is registered and preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 65917. The invention also provides a fermentation broth of the streptomyces, and an ethanol extract and a crude extract of the fermentation broth. The novel streptomyces, the crude extract thereof and the like have broad-spectrum antibacterial activity, have a good antagonistic effect on various pathogenic bacteria such as banana long leaf spot germs and banana colletotrichum gloeosporioides, can enable hyphae of the banana colletotrichum gloeosporioides to shrink, deform and break, inhibit spore germination of the banana colletotrichum gloeosporioides, and have a good anti-bacterial effect on the banana colletotrichum gloeosporioides. According to the present invention, with the application of the bacterial strain, the colletotrichum gloeosporioides spores are shrunk and broken, the growth of the colletotrichum gloeosporioides is inhibited, the peroxidase activity, the polyperoxidase activity, the CAT activity and the like in the banana peel can be improved, and the bacterial strain is the potential biological agent for the prevention and control of the colletotrichum gloeosporioides diseases, and has wide development space and good development and application prospects.

The invention relates to application of turmeric rhizome extract emulsifiable concentrate in preventing and treating corn leaf spot and tomato gray mold. Compared with the prior art, the turmeric rhizome extract missible oil disclosed by the invention contains an effective substance aromatic turmerone, and the aromatic turmerone can reduce the spore germination rate and sporulation quantity of pathogenic bacteria, increase the permeability of hypha cell membranes and cause overflow of contents, so that normal growth of hyphae is inhibited, and an antibacterial effect is achieved. Aromatic turmerone acts on plants, so that plant cells generate more polyphenol and flavonoid substances, and the immunity of the plants is improved. Pharmacodynamic test results in fields in two fields show that the 6.5% turmeric rhizome extract emulsifiable concentrate can be used for stably and effectively preventing and treating corn northern leaf blight and tomato gray mold after being diluted by 150-300 times, and also has a yield increasing effect.

The invention belongs to the technical field of biological control, and particularly relates to the technical field of crop stem rot control. The invention provides a bacillus velezensis Gc8, and the preservation number of the bacillus velezensis Gc8 is CGMCC (China General Microbiological Culture Collection Center) No. 28991. The bacillus velezensis Gc8 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 14, 2023, the preservation number of the bacillus velezensis Gc8 is CGMCC No.28991, the center is called CGMCC for short, and the address of the center is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention also provides a compound microbial agent, wherein the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67 and pseudomonas fluorescens pf27, and the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67 and pseudomonas fluorescens pf27, and the compound microbial agent comprises bacillus velezensis Gc8, kitasapora sp. Ak67, kitasapora fluorescens pf27, kitasapora fluorescens pf27 and kitasapora fluorescens pf27 and kitasapora fluorescens pf27 and kitasapora fluorescens pf27. The compound microbial agent can inhibit spore germination of pathogenic microorganisms, and has the effect of promoting growth of corn while resisting diseases.

The invention discloses a microbial agent and application thereof in preventing and treating rice blast and promoting rice growth, active ingredients of the microbial agent are formed by compounding cold bacillus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO: 33492 and bacillus velezensis with the preservation number of CGMCC NO: 33493, and the microbial agent aims at solving the synergistic problem of preventing and treating rice blast and promoting rice growth. According to the complex microbial inoculant, through the synergistic interaction between the two strains, the metabolite of the complex microbial inoculant can completely inhibit the spore germination of magnaporthe oryzae; meanwhile, the fungicide can degrade cellulose, obviously promote the growth of plant height, fresh weight and dry weight of rice, and realize the integration of disease prevention and growth promotion. The invention further discloses a preparation method of the fungicide and an application method of the fungicide for preventing and treating rice blast and promoting growth in a root irrigation mode in the three-leaf stage of rice. The microbial agent disclosed by the invention has dual functions of efficiently preventing diseases and stably promoting growth, and an efficient and safe biological solution is provided for green and sustainable development of agriculture.

Provided is a stabilized aqueous hydrogen peroxide composition comprising a sugar alcohol that does not contain silver, organophosphonate, peroxyacetic acid or other peracid, or stannate. The composition optionally includes a carboxylic acid. The composition is suitable as a disinfectant and oxidizer, and for the treatment, inhibition, or eradication of fungi or nematodes. Also provided is a method for reversing a bacterial or fungal infection in an infected plant, and a method for enhancing plant growth and / or productivity. Also provided is a method for inhibiting or preventing fungal spore germination in soil in a fungal infested area. Also provide is a method of controlling or preventing nematode infestation of a plant, and a method of reversing damage of a plant caused by nematode infestation. Also provided is a method for treating or reversing Panama disease in an infected banana plant.

The invention belongs to the technical field of plant growth-promoting bacteria, and particularly relates to protozoa trichomonad cooperating with bacillus to prevent and control cucumber fusarium wilt and application of the protozoa trichomonad. The invention provides a strain of protozoa trichomonas mtoleri (Cercomonas mtoleri) B5, and the preservation number of the strain is CCTCC (China Center for Type Culture Collection) N0: V202502. The protozoa trichomonad B5 provided by the invention can induce spore germination of a biocontrol strain bacillus velezensis SQR9 and improve the rhizosphere colonization capability of the biocontrol strain SQR9. The protozoa trichomonad B5 and the bacillus velezensis SQR9 are jointly inoculated in cucumber fusarium wilt continuous cropping soil, and the fusarium wilt morbidity of cucumber plants can be effectively reduced.

The present invention is in the field of agricultural pathogen control. Methods and compositions are described for controlling plant diseases caused by pathogens, including fungi and oomycete phytopathogens. Chemicals were screened for activity as autophagy inhibitors. The screening method used a bioluminescence resonance energy transfer (BRET)-based high-throughput screening strategy to identify compounds that inhibit fungal ATG4 cysteine protease-mediated cleavage of ATG8 for autophagosome formation. Ebselen and ebselen analogs were identified as inhibitors of fungal pathogens ATG4-mediated ATG8 processing. Identified chemicals inhibit spore germination, hyphal development, and appressorium formation. The autophagy inhibitors significantly reduced the virulence of pathogens in their corresponding plant hosts. The invention also provides for compositions comprising the same, methods of making the same, and methods of controlling plant disease.

The invention discloses an E3 ubiquitin ligase gene ulA, the nucleotide sequence of which is shown as SEQ ID NO: 1. The gene is knocked out from fusarium oxysporum by using a homologous recombination method to obtain a mutant strain ulA; compared with a wild strain, the mutant hulA has the advantages that the spore yield is reduced, the spore germination is delayed, the response capability to various abiotic stresses is reduced, the pathogenicity to panax notoginseng plants is reduced, and the sensitivity to six bactericides is increased, and experimental results show that the gene plays an important role in pathogenicity, drug resistance and the like of fusarium oxysporum. The gene can be used as a target gene for developing an anti-fusarium oxysporum preparation.

The invention discloses a hypha mineral composite bionic sand stabilization unit body and a preparation method thereof, and relates to the technical field of sand stabilization, the interior of the unit body is provided with a bionic three-dimensional porous skeleton structure, the unit body comprises a porous core layer, a porous core layer and a porous core layer, the porous core layer takes cleaning sand grains with the particle size of 0.1-2 mm as a sand-based carrier; uniformly mixing and curing with organic nutrient substances, mineral precursors and strain spores; the composite reinforcing layer is formed by interweaving and compounding a mycelium network formed by germination and growth of strain spores, mineral crystals generated by in-situ mineralization induced by metabolic activity of hyphae and a plant fiber reinforcing material which is externally added and embedded into the pores in the pores of the porous core layer; according to the hypha mineral compounded bionic sand stabilization unit body and the preparation method thereof, mold pressing or 3D printing prefabrication is adopted, the structure is controllable, and standardized production and transportation are facilitated; and through a controllable fermentation and gradient dehumidification mineralization culture process, hyphae and minerals are ensured to be uniformly and fully compounded in pores, and the product quality is stable.

The invention relates to a large-scale seedling raising method for rhizoma drynariae. The method comprises the steps of rhizoma drynariae greenhouse transplanting, seedling raising pretreatment, rhizoma drynariae spore collection, spore germination, rhizome formation promotion, conventional seedling management and the like, and a large number of seedlings can be obtained. According to the method, fortune's drynaria rhizome spores are promoted to germinate on a camphor tree sawdust substrate through weak illumination and a nutrient solution, and the problems that fortune's drynaria rhizome spores do not germinate due to water shortage and cannot form seedlings due to nutrition deficiency are effectively avoided The growth environment of the rhizoma drynariae cultivated in a greenhouse is fully utilized, and the seedling culture space is saved and the seedling culture cost is greatly reduced by modifying the seedling culture substrate and directly breeding spores; by means of an existing hanging spraying facility, water and nutrient supply for seedling raising is guaranteed, the labor cost is reduced, the spore germination rate and the seedling survival rate are greatly increased, and after small rhizomes are directly adopted for transplantation in subsequent management, the spore collection and sowing difficulty is reduced, and the seedling cultivation time is effectively shortened.

The invention relates to the technical field of biological control of plant diseases, in particular to application of a bacillus atrophaeus strain and a preparation thereof to control wheat gibberellic disease caused by gibberellic maize. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.29799, and the strain is classified and named as the bacillus atrophaeus strain HHQGTS13-1 (Bacillus atrophaeus HHQGTS13-1). The bacillus atrophaeus strain HHQGTS13-1 is wide in application range to natural environment conditions such as temperature and pH, and has the capability of antagonizing fusarium maydis when the temperature is 10-45 DEG C and the pH value is 4-10. The bacillus atrophaeus strain HHQGTS13-1 can be used for obviously inhibiting the growth of fusarium maydis hyphae and enabling the hyphae to be deformed; the antagonistic bacillus HHQGTS13-1 strain has an obvious inhibition effect on spore germination of fusarium maydis, the spore germination inhibition rate is 97.10%, the prevention and treatment effect of the antagonistic bacillus HHQGTS13-1 strain fermentation liquor after wheat is inoculated with fusarium maydis pathogenic bacteria is 63.43%, and the antagonistic bacillus HHQGTS13-1 strain has good potential of preventing and treating plant diseases caused by fusarium maydis.

The invention relates to a wheat gibberellic disease spore germination form detection method based on microscopic images. Compared with the prior art, the wheat gibberellic disease spore germination form detection method solves the defect that germination form spore microscopic images are difficult to detect. The method comprises the following steps: collecting and preprocessing a wheat scab spore microscopic image; construction of a germination form target key point detection model; training a germination form target key point detection model; acquiring and preprocessing a medicament treatment spore image; and outputting a spore detection result. Through the germination convolution cascade module and the germination recursion gating module, spore sub-pixel level details and a germination tube global contour are accurately captured in a microscopic image, the problem of missing detection and false detection of tiny targets is solved, and microscopic image accurate detection of germinated spores is realized.

The invention relates to an application of carboxylated chitin or carboxylated chitosan in promoting insect-killing fungi to prevent and treat diseases and insect pests. Research finds that carboxylated chitin or carboxylated chitosan can significantly promote spore germination and hypha growth of insecticidal fungi, also can significantly enhance stress resistance of the insecticidal fungi, significantly enhance toxicity of the insecticidal fungi to pests, improve ultraviolet resistance and high temperature resistance of the insecticidal fungi, and improve the insecticidal activity of the insecticidal fungi. The invention also finds that the carboxylated chitin or chitosan can effectively inhibit the growth of Xanthomonas oryzae pv. Oryzae. Therefore, the carboxylated chitin or the carboxylated chitosan can be used for preparing a product for enhancing the insecticidal fungus effect and a product for preventing and treating the rice bacterial leaf blight, and the prevention and treatment of plant diseases and insect pests are realized. In addition, carboxylated chitin and carboxylated chitosan are derived from natural polymer materials, have the characteristics of safety, no toxicity, biodegradability, environmental friendliness and the like, are environment-friendly and economical, and have wide application prospects and market values.

The invention belongs to the technical field of cibotium barometz breeding, and particularly relates to a method for artificially breeding cibotium barometz by using spores, which comprises the following components in parts by weight: 100 parts of field soil, 20-25 parts of sawdust, 10-15 parts of perlite, 10-12 parts of plant ash, 0.05-0.15 part of manganese sulfate and 0.3-0.5 part of sodium selenite; spores germinate, and the environment temperature is 24-26 DEG C; and transplanting the germinated seedlings into a greenhouse for cultivation. According to the method, high spore germination rate and seedling survival rate are ensured, and the transplanted seedlings can grow smoothly.

The invention discloses a method for cultivating kelp seedlings through diploid female gametophyte parthenogenesis, and belongs to the field of aquatic product engineering genetic breeding. And hybridizing the female and male single cell cloning lines of the induced protonema to obtain the tetraploid sporophyte. And the tetraploid sporophyte is mature and releases zoospores, diploid gametophytes are germinated and formed, and then male and female separation is performed to obtain diploid female gametophyte clone lines in batches. Under the culture condition provided by the invention, parthenogenesis of a diploid female gametophyte clone line is utilized for seedling culture. The diploid female gametophyte begins to ovulate after 6-7 days, and the ovulation time of the diploid female gametophyte is obviously earlier than that of haploid gametophyte in the same period and under the same condition. The 12-day ovulation rate reaches 86.5%, the 24-day sporophyte conversion rate reaches 98.3%, and most sporophyte forms are normal. In the seedling raising period, parthenogenesis seedling raising of the diploid female gametophyte is completed. The diploid female gametophyte can be used as a brand-new kelp germplasm material to be applied to kelp seedling cultivation.

The invention relates to the technical field of microorganisms, in particular to a pseudomonas koreensis LM for inhibiting fish pathogenic fungus water mildew and application of the pseudomonas koreensis LM. The pseudomonas koreensis LM is classified and named as Pseudomonas koreensis and is preserved in the China Center for Type Culture Collection on November 15, 2019, and the preservation number of the pseudomonas koreensis LM is CCTCC (China Center for Type Culture Collection) NO: M 2019934. The Korean pseudomonas LM provided by the invention has good inhibitory activity on hypha growth and spore germination of fish pathogenic fungi saprolegnia, is stable in bacteriostatic activity and good in biological safety, and has a potential value of being developed into a green and environment-friendly saprolegnia biological prevention and control agent.

The invention discloses bacillus and application thereof, and belongs to the technical field of microorganisms, the bacillus is preserved in China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, China, the preservation number is CCTCC NO: M 20242290, and the preservation date is October 22, 2024. The composition can effectively inhibit vegetative growth and vegetative propagation of pathogenic bacteria such as colletotrichum gloeosporioides and paecilomyces japonicus, and has a good and broad-spectrum control effect on various pathogenic fungi. Bacteriostatic components contained in fermentation filtrate of the bacillus can remarkably inhibit germination of colletotrichum spruce spores and formation of appressorium. And the bacillus has a good prevention and treatment effect on the citrus huanglongbing.

The embodiment of the invention provides a grain fungal disease early warning method, device and equipment and a computer storage medium. The early warning method for the fungal diseases of the grains comprises the following steps: constructing a fungal germination index model according to a coupling relationship between canopy temperature and leaf surface humidity of the grains; inputting canopy temperature data and leaf surface humidity data of grains into the fungal germination index model to obtain a fungal spore germination probability; and when the fungal spore germination probability meets a threshold condition, generating early warning information of cereal fungal diseases. According to the embodiment of the invention, by constructing the fungus germination index model driven by coupling of the canopy temperature and the leaf surface humidity, the biophysical parameters of the grains are quantified into the fungal spore germination probability, the physiological reactions of fungi in different environments are monitored in real time in the early warning process of the fungal diseases of the grains, and the early warning accuracy of the fungal diseases of the grains is improved.

The invention relates to the technical field of cyanobacterial bloom control in water, and particularly discloses a compound enzyme-bacterium synergistic preparation for cyanobacterial bloom control, a preparation method and a use method. The preparation comprises the following components in percentage by mass: compound enzymes (10-20% of cellulase, 8-12% of beta-1, 3-glucanase and 3-8% of lipase), functional bacteria (10-20% of bacillus subtilis, 8-15% of trichoderma harzianum and 5-10% of bacillus amyloliquefaciens), an enzyme stabilizer (3-8% of trehalose), an adsorption carrier (15-25% of Fe < 3 + > modified charcoal and 8-15% of diatomite) and auxiliary components. The preparation method comprises the steps of carrier modification, solid fermentation and microcapsule embedding. During use, 3-20kg / 1000m < 3 > of water is added for the first time, 2-10kg / 1000m < 3 > of water is added every 7-14 days in the maintenance stage, and synchronous aeration is performed. Through the synergistic effect of beta-1, 3-glucanase and trichoderma harzianum, the algicidal rate is 92%, and the spore germination inhibition rate is 82%; the Fe < 3 + > modified charcoal improves the stability of the enzyme in an alkaline environment (80% of activity is retained when the pH value is 9); the method is suitable for eutrophic water with chlorophyll a greater than or equal to 30 [mu] g / L, and has no chemical residue risk.

The invention belongs to the technical field of agricultural control, and particularly relates to a pesticide for inhibiting fusarium graminearum as well as a preparation method and application thereof. The pesticide is a chitosan / chestnut flower polysaccharide composite material, and the chitosan / chestnut flower polysaccharide composite material is obtained by forming nucleophilic substitution between-COOH in chestnut flower polysaccharide and-NH2 in chitosan and generating amido bonds; the chestnut flower polysaccharide is obtained through a water extraction and alcohol precipitation method. As a pesticide, the chitosan / chestnut flower crude polysaccharide composite material has a good antibacterial rate, EC50 of fusarium graminearum hypha growth and spore germination are 442.6318 mu g / mL and 8.3516 mu g / mL respectively, in addition, the chitosan / chestnut flower crude polysaccharide composite material can promote germination of wheat seeds, the content of photosynthetic pigments in leaves is increased, and the yield of the wheat seeds is increased. And the activity of crop pathogenic bacterium defensive enzyme is improved.

The invention discloses a polysaccharide polymer, application of the polysaccharide polymer and a preparation method of the polysaccharide polymer. The polysaccharide polymer QPS capable of remarkably selectively inhibiting harmful bacteria in aquaculture is separated and purified from the water mold antagonistic actinomycete strain QHV2 for the first time, can effectively inhibit mycelial growth and spore germination of water mold / branchiomycosis and destroy mature biological membranes, is stable in activity, free of toxic effects on fry and good in biological safety, and can be applied to the field of aquaculture. The material can be used as a candidate material for research and development of a fish pathogenic fungus biological prevention and control agent. Meanwhile, the polysaccharide polymer QPS avoids pollution of harmful substances to a water body when a QHV2 fermentation product is directly applied, can selectively inhibit staphylococcus aureus, saprolegnia, branchiomycosis and other harmful bacteria in aquaculture, and has no inhibition or weak inhibition on pseudomonas pseudoalcaligenes, bacillus and other beneficial bacteria in aquaculture. And the application effect is better.

A process 100 for the decontamination and microbiological stabilisation of liquid egg white stored at room temperature, comprising the operating steps of: preparing the egg white in a liquid state; carrying out a first thermal treatment 110 by carrying out a first and a second sub-step for heating to specific temperature ranges and for specific time intervals in order to eliminate part of the microorganisms present in the egg white and activate any spores present; incubating 130 the egg white at a pre-established temperature and for a specific incubation time interval for the germination of any spores present; carrying out a second thermal treatment 140 on the egg white equivalent to the first to eliminate the spore-derived bacteria; carrying out an isothermal treatment 160 of the egg white to eliminate the bacteria present in the egg white that were not eliminated by the second thermal treatment; carrying out a third thermal treatment 170 similar to the aforesaid first and second thermal treatment in order to eliminate the bacteria present in the egg white that were not eliminated by said first and second thermal treatments.

The application provides a strain of antagonistic cucumber downy mildew Microbacterium luteum C10-2 and application thereof, and belongs to the technical field of biocontrol bacteria. The Microbacterium luteum C10-2 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20253025. The strain can effectively inhibit cucumber downy mildew. Indoor test shows that the spore germination inhibition rate of the strain on Pseudoperonospora cubensis is 86.54%, the leaf disc prevention efficiency is 84.23%, and the strain is superior to the chemical agent dimethomorph; the fermentation liquor of the strain is sprayed in a field at a concentration of 1x10 8 cfu / mL, and the prevention efficiency reaches 80.50%. The strain can stably colonize in cucumber, inhibit pathogenic bacteria infection, and is environment-friendly, free of drug hazards and not prone to drug resistance. The application provides a new strain resource for green prevention and control of cucumber downy mildew, and has a wide application prospect.

The invention discloses a Ustilaginoidea virens inoculant, a preparation method and application of the Ustilaginoidea virens inoculant in Ustilaginoidea virens inoculation, and belongs to the technical field of agricultural microorganisms. According to the method, two-step shake-flask culture is combined with the steps of filtering, centrifuging and spore germination, so that the ustilaginoidea virens inoculum taking germinated spores (GC) as the core is prepared, and the problems of hypha aging, low spore activity, asynchronous infection and the like in the existing inoculation method are effectively solved. The inoculant has a tender hypha structure and strong infection activity, and the inoculation efficiency and the pathogenesis consistency are remarkably improved. When being used for preparing transcriptome samples, metabolome samples and other omics samples, the strain avoids non-specific immune interference caused by PAMPs released by broken hyphae, ensures synchronous infection process, and is real and reliable in immune response. The invention provides a stable and efficient inoculation system with a clean background for the research on the ustilaginoidea virens-rice interaction mechanism, and has a good application prospect.

The utility model provides a fern spore germination incubator, and relates to the technical field of fern spore cultivation. The spore culture device is used for solving the technical problems that the spore culture device provided by the existing patent cannot ventilate the interior of the box body, and the spore culture is easily influenced. A fern spore germination incubator comprises a placement cabinet, sliding doors, placement plates and culture boxes, the two sliding doors are arranged on the placement cabinet in a sliding mode, the two placement plates are installed in the placement cabinet, a plurality of vent holes are formed in the placement plates, the culture boxes are placed at the bottom in the placement cabinet and on the placement plates, and a plurality of vent holes are formed in the top of the placement cabinet. A pull rod translates leftwards and rightwards, so that a rotating block drives a connecting rod to press downwards towards one side, at the moment, two ventilation plates rotate at a ventilation opening, gaps are formed between the ventilation plates and the ventilation opening, air inside and outside the placement cabinet can circulate at the moment, and the effect of controlling ventilation in the placement cabinet is achieved.

The present invention pertains to use of a composition comprising propionic acid and / or salt thereof and at least one fatty acid having 6 to 12 carbon atoms for reducing dormant spore viability, for inactivating dormant conidia, for eliminating or reducing germination of conidia, for increasing cell damage and / or cell death of hyphae, and / or for reducing or eliminating germ tube formation.

The invention discloses paenibacillus FHY1 and application thereof, and belongs to the field of microorganisms. A strain of paenibacillus FHY1 is screened from Xinjiang populus euphratica juice, and experimental verification shows that a liquid culture of the paenibacillus FHY1 strain provided by the invention has a remarkable inhibition effect on magnaporthe oryzae, the bacteriostasis rate can reach 84%, and the inhibition rate on magnaporthe oryzae spore germination is 92%. And the rice growth can be promoted under the conditions of 150mM NaCl and 250mM NaCl. Meanwhile, the strain also has a good inhibition effect on pyricularia grisea, fusarium oxysporum, fusarium graminearum, sclerotinia sclerotiorum and colletotrichum gloeosporioides. Therefore, the invention provides a new strain with a good control effect on pathogenic fungi, and meanwhile, the growth and development of rice are remarkably promoted in a salt stress environment.

The present application provides a novel structure of thiophene compound, the compound has significant inhibitory effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as Pyricularia oryzae, Colletotrichum gloeosporioides, Gibberella, Phytophthora, Phytophthora capsici), which can be used for preventing and treating plant diseases caused by fungi and / or oomycetes.

The invention discloses preparation and application of a double-component dsRNA (double-stranded ribonucleic acid) medicament for enhancing resistance mutation risk capability of RNA (ribonucleic acid) pesticide to botrytis cinerea, and a key pathogenic gene of botrytis cinerea is any one or two of a botrytis cinerea global regulatory factor Vel1 gene or a galacturonic acid reductase Gar2 gene. The dsRNA is any one or two of dsRNAs targeting a Vel1 gene or a Gar2 gene, and efficient prevention and control of gray mold are achieved through key pathogenic links such as spore germination inhibition, hypha colonization and infection structure formation. In addition, the effectiveness of the RNA pesticide aiming at botrytis cinerea after single target gene mutation is evaluated through cooperative use of the combination of the double-component RNA, the ds-Gar2 and the ds-Vel1, the good effect of the double-target synergistic effect in the aspect of reducing the off-target risk is proved, and the practice and popularization of a green prevention and control technology in prevention and control of gray mold are promoted.

The present application provides a kind of streptomyces, named Streptomyces sp., is registered and preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No:65917.The present application also provides fermentation broth of the streptomyces and ethanol extract and crude extract of fermentation broth thereof.The new species of streptomyces and its crude extract and the like of the present application have broad-spectrum antibacterial activity, have good antagonism to many pathogenic bacteria such as banana long-shaped spot fungus and banana anthracnose fungus, and can make banana anthracnose fungus hyphae wrinkle, deform, break, inhibit banana anthracnose fungus spore germination, make banana anthracnose fungus spore germination wrinkle, break, inhibit banana anthracnose fungus growth, and can also improve peroxidase, polyperoxidase, CAT activity and the like in banana peel, is a potential biological agent for preventing and treating anthracnose disease, stem rot disease and the like, has broad development space, and has good development and application prospect.