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Antimicrobial, antibacterial and spore germination inhibiting activity from an avocado extract enriched in bioactive compounds

an avocado extract and bioactive compound technology, applied in the direction of antibacterial agents, drug compositions, detergent compounding agents, etc., can solve the problems of inability to successfully identify components, no specific antimicrobial properties of spore forming bacteria tested by cited authors, and no method to produce acetogenins. to achieve the effect of inhibiting the growth of viable cells

Inactive Publication Date: 2013-08-22
INST TECHNOLOGICO & DE ESTUDIOS SUPERIORES DE MONTERREY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent is about a special plant extract that contains certain chemicals that have antimicrobial, antibacterial, and spore germination inhibiting effects. The extract can be used as a sporicidal and sporostatic agent against harmful bacteria. It can also be used in pharmaceutical, food, personal care, and cleaning products to prevent the growth of harmful bacteria. The patent also mentions the discovery of two new chemicals in the extract.

Problems solved by technology

Therefore the prior cited studies did not successfully performed the isolation or chemical identification of the components potentially responsible for the observed bioactivities or tested bacterial spores, heat-shocked spores or pressure-shocked spores.
Even if some acetogenins have been proven to have antimicrobial activity against vegetative cells of bacteria, the preliminary art does not show any reports on the bio-assay guided isolation of the antimicrobial compounds from avocado (Persea americana) against microorganisms, particularly sporulated forms.
None of the cited authors tested any specific antimicrobial properties against spore forming bacteria nor a method to produce acetogenins with this particular effect.
Furthermore, they have demonstrated synergism in antimicrobial activity when used in combination with organic acids (malic, tartaric acid, benzoic acids etc.), bacteriocins like nisin or chelating agents like EDTA in various model systems including fresh products (fruits and vegetables), raw and ready-to-eat meat and poultry products.

Method used

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  • Antimicrobial, antibacterial and spore germination inhibiting activity from an avocado extract enriched in bioactive compounds
  • Antimicrobial, antibacterial and spore germination inhibiting activity from an avocado extract enriched in bioactive compounds
  • Antimicrobial, antibacterial and spore germination inhibiting activity from an avocado extract enriched in bioactive compounds

Examples

Experimental program
Comparison scheme
Effect test

example 1

Antimicrobial and Sporicidal Activity of Acetone and Hexane Avocado Seed Extracts

[0097]Avocado seeds were ground using a colloidal mill to obtain particles with an average radius of 0.5-2 mm. Ground avocado seeds (50 g) were mixed with either acetone or hexane at a material-to-solvent ratio of 1:2 (w / v). Mixtures were stored for 24 hr at 25° C. in order to obtain an avocado seed raw extract. The seed was separated from the extract by means of vacuum filtration. The raw extracts were evaporated under vacuum to dryness using a rotary evaporator (35° C., 22 in Hg) and the obtained dry matter was weighed and redissolved in acetone to a final concentration of 2.5 mg / ml. Adjusted samples were used for antimicrobial and sporicidal tests (see FIG. 1).

[0098]For the antibacterial evaluations, adjusted solutions (5 μg) were transferred to sterile 6-mm diameter discs made from Whatman no. 1 filter paper, so that after solvent evaporation each disc contained 12.5 μg of solids from the enriched a...

example 2

Specific Activity of Avocado Seed Extracts Against Vegetative Cells and Heat-Shocked Bacterial Spores of Spore-Forming Bacteria as Compared to Other Plant Sources

[0101]The efficacy of the present disclosure can be observed by the preparation of crude antibacterial extracts from mango seed kernel, which has been reported in the prior art to exhibit antibacterial activity against vegetative cells of spore-forming bacteria (Kabuki et al., 2000).

[0102]Crude extracts from avocado (Persea americana) and mango kernel (Mangifera indica) were prepared as described in Example 1 and their antibacterial activities tested against the growth of vegetative cells and heat-shocked spores of C. sporogenes (See Table 1).

TABLE 1Antibacterial activities of avocado seed and mango kernelextracts against vegetative cells and heat shocked spores ofClostridium sporogenes (ATCC 7955).Antibacterial Activity againstExtract(Disc inhibition zone (cm))ConcentrationVegetativeHeat-shockedPlant Source(mg / mL)cellsspor...

example 3

Effect of Shaking on the Antimicrobial Activities of Crude Acetone and Hexane Avocado Seed Extracts

[0105]Similarly to Example 1, avocado seeds were ground using a colloidal mill obtaining particles with an average diameter of 0.5-2 mm. Ground avocado seeds (50 g) were mixed with hexane at a material-to-solvent ratio of 1:2 (m / v). Mixtures were shaken or soaked at 200 rpm for 24 hr at 25° C. in order to obtain an avocado seed raw extract. The raw extracts were evaporated to dryness using a Rotary evaporator (35° C., 22 in Hg) and the obtained dry matter was weighed.

[0106]As in Example 1, dry matter was re-dissolved in acetone to a final concentration of 2.5 mg / ml for the antibacterial evaluations. Clostridium sporogenes (ATCC 7955) was used as test microorganism since it is a known surrogate microorganism for Clostridium botulinum. Antimicrobial activities against vegetative bacterial cells, as well as native and heat-shocked spores were conducted as described in Example 1.

[0107]A si...

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Abstract

The present disclosure relates to extracts from Persea sp. (avocado) enriched in bioactive compounds which can be used as antimicrobial, antibacterial or spore germination inhibiting agents, the process for obtaining the extracts, acetogenins and isolated molecules and methods for using the extracts enriched in bioactive compounds for providing antimicrobial, antibacterial or spore germination inhibiting effect.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation of International PCT Application No. PCT / IB2011 / 053535 filed Aug. 8, 2011. This application also claims priority to U.S. Provisional Application No. 61 / 371,984 filed Aug. 9, 2010. The contents of all of the above are hereby incorporated in their entirety by reference.[0002]Any foregoing applications and all documents cited therein or during their prosecution (“application cited documents”) and all documents cited or referenced in the application cited documents, and all documents cited or referenced herein (“herein cited documents”), and all documents cited or referenced in herein cited documents, together with any manufacturer's instructions, descriptions, product specifications, and product sheets for any products mentioned herein or in any document incorporated by reference herein, are hereby incorporated herein by reference, and may be employed in the practice of the disclosure.BACKGROUND[0003]1. Tec...

Claims

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Application Information

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IPC IPC(8): A01N37/12
CPCA61K31/12A61K31/23A01N65/00A01N37/12A61K2236/39A61K36/54A61K8/97A61Q17/005A61K45/06A61K2300/00A01N65/24A01N2300/00A23L3/34635A23L3/3508A23L3/3526A23L3/3544A23L3/358A61K8/375A61K31/22A61Q17/04A61Q19/001A61Q19/10C07C69/16C11D3/2093C11D3/48C11D7/266C11D9/26A23V2002/00A23V2200/10A23V2250/708A61K8/9789A61P31/00A61P31/04Y02A50/30A23L3/3499A61K8/37A61K2236/33A61K2236/35A61K2800/524A61Q19/00C07C69/145
Inventor HERNANDEZ-BRENES, CARMENGARCIA-CRUZ, MARIA ISABELGUTIERREZ-URIBE, JANET ALEJANDRABENAVIDES-LOZANO, JORGE ALEJANDRORODRIGUEZ-SANCHEZ, DARIANA GRACIELA
Owner INST TECHNOLOGICO & DE ESTUDIOS SUPERIORES DE MONTERREY
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