The invention discloses an
induction method for obtaining a transgenic
persea americana composite
plant without
tissue culture, and belongs to the technical field of
persea americana
biology. The
induction method provided by the invention comprises the following steps: germinating avocado seeds by using
quartz sand to obtain avocado seedlings, infecting rootless seedlings from the avocado seedlings by adopting a vacuum infiltration method, and inserting the rootless seedlings subjected to dip
dyeing into a sterilized coco
coir matrix for culturing to obtain the avocado composite
plant. According to the method, an efficient avocado genetic transformation
system mediated by
agrobacterium rhizogenes is established,
tissue culture is not needed, rootless seedlings are adopted as materials, the
transformation efficiency is improved, meanwhile, consumption of experimental equipment,
consumables and manpower is reduced, the problem that the avocado genetic transformation
system is not established yet is solved, and the method is suitable for popularization and application. The technical effect of quickly, efficiently, simply and easily obtaining the transgenic material with low consumption is achieved, and a more convenient and efficient way is provided for genetic transformation of avocados.