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105 results about "Fruit maturation" patented technology

Tomato, banana and avocado are examples of fruit that is mature at harvest, yet rather inedible until further ripening. Strawberries, oranges, boysenberries and grapes are fruits that need to complete the fruit maturation process on the plant.

Orchard crawler differential robot trajectory tracking method based on adaptive incremental control

The invention discloses an orchard crawler differential robot trajectory tracking method based on adaptive incremental control, and relates to the technical field of agricultural robot control. The method comprises the following steps: receiving environmental data collected by a robot sensor, constructing a probability grid map of an orchard environment according to the environmental data, and aligning the probability grid map with a pre-constructed map inertial coordinate system; the course angle error and the distance error between the current position and a target point are calculated based on the pose of the robot, an error vector is formed, and the target point is dynamically planned according to the fruit maturity and reachability priority detected by a visual system and a laser radar point cloud. According to the method, coordinate transformation is not needed, and different from traditional tracking control, a control instruction is generated directly based on pose increment, the course and distance error of a target point is calculated directly based on an inertial coordinate system, and real-time matrix projection operation is avoided.
Owner:SOUTHEAST UNIV

Application of transcription factor CaNAC2 in regulation and control of pepper fruit maturity

PendingCN120818553APlant peptidesFermentationBiotechnologyCarotene metabolism
The invention discloses an application of a transcription factor CaNAC2 in regulation and control of pepper fruit maturity. The invention relates to the technical field of plant genetic engineering, and provides any one of the following applications of CaNAC2 protein: regulating and controlling ripening of plant fruits; the carotenoid content in the plant fruits is regulated and controlled; the CaNAC2 protein is a protein of which the amino acid sequence is SEQ ID NO: 1. A target fragment of the CaNAC2 gene is amplified in the pepper, a VIGS vector is constructed, and it is found that inhibition of expression of the CaNAC2 gene can regulate the content of carotenoid in pepper fruits and delay fruit maturity, so that the shelf life of the pepper is prolonged. Therefore, the CaNAC2 gene plays an important role in metabolism of capsicum carotenoid, and has important significance on appearance and market value of fruits.
Owner:CHINA AGRI UNIV

FaCP5L1 and FaCP5L3 genes and their application in regulating fruit ripening and postharvest preservation quality.

The FaCP5L1 and FaCP5L3 genes and application thereof in regulating fruit ripening and postharvest quality belong to the field of agricultural biological genetic engineering technology. It is found for the first time that the cruciferin coding genes FaCP5L1 and FaCP5L3 can directly participate in the regulation of strawberry ripening and postharvest quality, including but not limited to the regulation of abscisic acid (ABA) content, ripening process, soluble solid content, hardness, color, anthocyanin content. The expression of FaCP5L1 and FaCP5L3 can promote the ripening of strawberry fruit and the formation of postharvest quality; the expression reduction can slow down the ripening of strawberry fruit and the formation of postharvest quality. Accordingly, the ripening of strawberry fruit and the postharvest quality traits can be regulated by reasonably utilizing the genes, and the ripening and postharvest quality related breeding can be guided. The present application provides a theoretical basis and technical support for regulating the ripening of strawberry fruit and the postharvest quality traits by using genetic engineering technology, and has important application value.
Owner:ZHEJIANG UNIV

Olive fruit maturity detection method and system

The invention discloses an olive fruit maturity detection method and system, and the method comprises the steps: firstly constructing a first standard model and a second standard model, then obtaining a comparison image and an actual hardness calculation parameter of a to-be-detected olive fruit, and calculating a color degree score of the olive fruit according to the comparison image and the first standard model, calculating a hardness score according to the actual hardness calculation parameter and a second standard model, and judging the maturity of the olive fruits according to the color score and the hardness score; olea europaea fruits correspond to different color changes and different hardness changes in different ripening stages, the surface layer changes of the fruits are reflected through the color changes, the internal changes of the fruits are reflected through the hardness of the fruits, the fruits are evaluated at the same time from the inside and the outside, and the evaluation accuracy is improved. Misjudgment caused by premature color change or abnormal change of the fruits can be effectively avoided, and the accuracy of maturity detection is improved; the determination of the fruit maturity can be standardized, and the dependence of the determination of the fruit maturity on manpower is reduced.
Owner:XICHANG COLLEGE

MaHDAC3 gene and application thereof in regulating and controlling fruit ripening

The invention provides an MaHDAC3 gene and application thereof in regulating and controlling fruit maturity, and belongs to the technical field of gene engineering. The nucleotide sequence of the MaHDAC3 gene is as shown in SEQ ID NO.1. The banana fruit MaHDAC3 gene is silenced by using a VIGS technology, and the result shows that the hardness, soluble sugar, ROS content and other fruit ripening and softening related physiological indexes of the fruit with the silenced banana MaHDAC3 gene are significantly changed, which indicates that the MaHDAC3 gene is a key gene influencing the ripening and softening of the sweet banana fruit, and the gene has a significant effect on the ripening and softening of the sweet banana fruit. The method can be applied to control of maturation or softening of banana fruits and cultivation of new banana varieties.
Owner:SOUTH SUBTROPICAL CROP RES INST CHINA ACAD OF TROPICAL AGRI SCI

An orchard single fruit picking optimization method based on multi-modal data fusion

This invention relates to an optimized method for single-fruit harvesting in orchards based on multimodal data fusion. It involves simultaneously acquiring image information of the fruit, local environmental data, and tree physiological indicators using multimodal sensors, and preprocessing these data. Taking into account the heterogeneous nature of different modalities, independent feature extraction networks are used to obtain deep features for each modality, constructing a heterogeneous modal deep fusion network. A cross-domain attention mechanism dynamically integrates these features, effectively capturing the complex correlations affecting fruit maturity and generating fused features. Based on these features, the model can predict the real-time maturity of individual fruits. Combining external conditions, a multi-objective optimization algorithm is used to calculate the harvesting priority score for each fruit, generating an executable harvesting sequence to guide robots or manual harvesting in an orderly manner. This invention significantly improves the accuracy of maturity prediction and the overall efficiency of harvesting operations, providing effective support for refined management and intelligent operation in orchards.
Owner:CETC BIGDATA RES INST CO LTD

Application of FveABI4 in regulating and controlling sizes of forest strawberry fruits

The invention provides application of FveABI4 in regulating and controlling the size of forest strawberry fruits, and belongs to the technical field of genetic engineering. Researches find that overexpression of FveABI4 can increase the size of fruits, promote the ripening process of the fruits, improve accumulation of soluble sugar in the fruits and reduce the content of citric acid in the fruits; and the reverse effect can be achieved by knocking down or knocking out the FveABI4. Therefore, the fruit size is regulated and controlled by intervening the expression of the FveABI4, and the ripening process and the sugar acidity of the fruits can be controlled at the same time, so that the problems that the inherent fruits of the forest strawberries are small and the separation of excellent characters of traditional breeding is serious can be ideally solved, and the application potential of the forest strawberries in the aspects of edible characters, processing characters, ornamental characters and the like is fully released.
Owner:CHINA AGRI UNIV

SNP (Single Nucleotide Polymorphism) molecular marker related to pitted surface of pepper and application

The invention relates to the technical field of plant molecular biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to a pitted surface of pepper and application. The SNP molecular marker can stably and accurately distinguish pitted surface (G / G), smooth surface (C / C) and heterozygous (G / C) genotypes of capsicum fruits, the marker typing and phenotype consistency is high, the accuracy rate reaches 90% or above, and the SNP molecular marker is remarkably superior to an existing phenotype-dependent observation method. Moreover, the SNP molecular marker can be used for identifying the pitted surface character of the fruit in the early stage of the plant, so that character judgment after fruit ripening is avoided, the breeding period can be effectively shortened, and the breeding efficiency can be improved. The SNP molecular marker can be directly applied to molecular breeding of pepper pitted surface characters, can provide reliable technical support for breeding of high-quality varieties, and has high practical value and popularization and application prospects.
Owner:CHINA AGRI UNIV SANYA RES INST +1

A live sowing cultivation method for radix stemonae

The application discloses a kind of stachys seed direct sowing cultivation methods, belong to stachys cultivation technical field, the cultivation method is as follows: (1) selection: when fruit ripens, seed is collected, removes fruit pod, and seed and oil body are separated and collected for future use;(2) seed pretreatment: the stachys seed collected is pretreated to obtain germinated seed;(3) planting site treatment: after the mixed fertilizer is evenly applied to the surface of planting site, it is watered, penetrates 8-12 days, and is ploughed after overall and is ridged;(4) sowing: germinated seed is sown in the treated planting site by direct sowing method.The application adopts the method of direct sowing cultivation, avoids the damage to seedling caused by transplanting process when stachys is planted, solves the problem of low germination rate of stachys seed by sowing after treating stachys seed, and improves the yield and quality of stachys.
Owner:CHONGQING ACAD OF CHINESE MATERIA MEDICA

Molecular marker probe combination for auxiliary selection of characters in mature period of apple fruits and application of molecular marker probe combination

The invention belongs to the technical field of plant molecular breeding, and particularly relates to a molecular marker probe combination for auxiliary selection of characters in an apple fruit maturation period and application of the molecular marker probe combination. The probe combination comprises eight probes for auxiliary selection of characters in the mature period of the apple fruits; the nucleotide sequences of the eight probes for auxiliary selection of the characters of the apple fruits in the mature period are sequentially shown as SEQ ID NO.9 to SEQ ID NO.16. The DNA probe combination corresponding to the SNP marker combination provided by the invention can be applied to molecular marker-assisted selection of mature characters of apples in the seedling stage of apple filial generations.
Owner:CHINA AGRI UNIV

A method for reducing chemical sensing substances and increasing polysaccharide content in polygonatum sibiricum planting

The present application provides a kind of planting method of reducing allelochemicals and improving polysaccharide content of Rhizoma Polygonati, belongs to plant cultivation technical field.The planting method is planted in acidic sandy loam soil, and "biochar C" is applied to the soil 1-2 times in September-October of the second and third years, and the fruits mature, the stems are laid down after the third year, and then it can be harvested."Biochar C" is a modified, loaded with salicylic acid and UDP-D-galactose biochar.The present application not only helps to improve the physicochemical properties and bacterial community diversity of Rhizoma Polygonati planting soil, thereby reducing the allelochemicals content of Rhizoma Polygonati rhizome, but also can improve the polysaccharide content of rhizome, which helps to improve the quality of Rhizoma Polygonati and enhance its efficacy.
Owner:CROP RES INST OF FUJIAN ACAD OF AGRI SCI

Method for advancing fruit maturation period of snowflake pears

The invention discloses a method for advancing the fruit maturation period of snowflake pears, and relates to the technical field of fruit and vegetable planting. According to the method, 15 days before fruit harvesting, a fruit ripening promoting agent is sprayed to fruit trees; the fruit maturation promoting agent is any one of 200 [mu] mol / L melatonin, 100 [mu] mol / L jasmonic acid or a mixture of 200 [mu] mol / L melatonin and 100 [mu] mol / L jasmonic acid. According to the method, the arbutin content and the soluble solid content in the early-harvested snowflake pear fruits can be increased, the hardness and the titratable acid content are reduced, and the change of the indexes is synthesized to powerfully prove that the snowflake pears are matured in advance.
Owner:HEBEI AGRICULTURAL UNIV.

A method for monitoring a fruit bottom colored reflective film

The present application belongs to the technical field of intelligent agriculture, and discloses a monitoring method for a fruit bottom colorizing and light reflecting film, comprising the following steps: S1, forming a grid map for an orchard; S2, collecting fruit bottom images to obtain array type multi-angle image data; S3, performing edge processing on the image data to form fruit images; S4, performing fruit surface color analysis on the fruit images to form color feature data; S5, performing edge and dispersion analysis on dark color patches in the color analysis process to form lesion feature data; S6, inputting the color feature data and the lesion feature data of S4 and S5 into a preset fruit maturity and lesion grade library to obtain preliminary maturity and sunscald grades; S7, performing feature weighting judgment to output maturity grades and / or sunscald lesion probabilities; S8, if the lesion probability is greater than a preset threshold, automatically recording a collection time stamp, a lesion probability value, an image slice and a map location, and triggering an early warning of adjusting the light reflecting film to weaken the radiant light; if the lesion probability is less than or equal to the preset threshold, normally recording data.
Owner:QINGDAO AGRI UNIV

Functional gene pa spl8 for regulating apricot fruit ripening and application thereof

The application discloses a functional gene PaSPL8 for regulating apricot fruit ripening, and the nucleotide sequence of the PaSPL8 gene is shown as SEQ ID NO. 1. The functional gene PaSPL8 for regulating apricot fruit ripening is used for regulating apricot or tomato fruit ripening, and the fruit ripening is delayed by increasing the expression amount of the PaSPL8 gene; or the functional gene PaSPL8 for regulating apricot fruit ripening is used for regulating the content of abscisic acid in plant tissues. The application verifies that the PaSPL8 gene regulates the ripening of apricot fruits by regulating the content of ABA in apricots, and therefore, the PaSPL8 gene can be used for apricot breeding.
Owner:RES INST OF NON TIMBER FORESTRY CHINESE ACAD OF FORESTRY

Chitosan / lysozyme amyloid protein fiber composite microneedle based on ethephon controlled release and application of chitosan / lysozyme amyloid protein fiber composite microneedle in on-demand ripening acceleration of picked fruits

PendingCN121264487ABiocideStampsFruit maturationEthephon
The invention discloses a chitosan / lysozyme amyloid protein fiber composite microneedle based on ethephon controlled release and application of the composite microneedle in on-demand ripening acceleration of harvested fruits, and belongs to the technical field of agricultural product after-harvest treatment. The composite microneedle is composed of chitosan, lysozyme amyloid protein fibers and ethephon, the mass ratio of the chitosan to the lysozyme amyloid protein fibers is 5: 5, and the ethephon loading capacity is that 131.13 [mu] L of a 40% ethephon solution is added to every 17.5 mL of a 1% chitosan solution. The microneedle can be inserted into the epidermis of picked fruits to realize on-demand slow release of ethephon and promote ripening of the fruits, meanwhile, the lysozyme amyloid protein fiber provides an antibacterial effect, the aging process of the fruits after ripening is effectively delayed, the infection rate of colletotrichum gloeosporioides is reduced, and the ethephon residue in the fruits is remarkably lower than the national limited standard. The method solves the problems of non-uniform fruit ripening, excessive softening and potential toxicity caused by a traditional ethephon ripening method, and realizes integration of batch on-demand ripening and preservation of picked fruits.
Owner:HAINAN UNIV

Fruit ripening detection method and electronic equipment

The invention discloses a fruit ripening detection method and electronic equipment, and the fruit ripening detection method comprises the steps: determining the first ripening degree of a to-be-detected fruit according to the volatile gas concentration of the to-be-detected fruit; determining the second maturity of the to-be-detected fruit according to the visual features of the to-be-detected fruit; determining a third maturity degree of the to-be-detected fruit according to the fruit volume of the to-be-detected fruit; and according to the first maturity, the second maturity and the third maturity, determining whether the to-be-detected fruit is mature. According to the method, the fruit ripening detection of the to-be-detected fruit is rapidly carried out based on the volatile gas concentration, the visual characteristics and the fruit volume of the to-be-detected fruit, and the detection efficiency and the detection accuracy of the fruit ripening detection of the to-be-detected fruit are improved.
Owner:深圳市天天学农网络科技有限公司

A method for cultivating selenium-rich blueberries

This invention belongs to the field of agricultural and forestry plant cultivation technology. More specifically, it relates to a method for cultivating blueberries with selenium enrichment. The specific cultivation steps of this invention include: substrate selection: selecting a cultivation substrate or soil with a selenium content ≥0.2mg / kg; blueberry variety selection: selecting blueberry varieties such as Brilliant, with a flowering period of ≥50 days from the start of flowering to fruit ripening; planting the blueberry plants in the cultivation substrate or soil, and fortifying the blueberry plants with rhizosphere selenium nutrition or foliar selenium nutrition; the rhizosphere selenium nutrition fortification includes: applying the first selenium fertilizer in furrows 15-30 days before flower bud emergence; the foliar selenium nutrition fortification includes: spraying the second selenium fertilizer 5-10 days before or after fruit enlargement.
Owner:YICHUN ACAD OF SCI (JIANGXI SELENIUM-RICH IND RES INST)

Application of the tomato SlPIF8b gene in regulating tomato fruit coloring and ripening

This invention discloses the application of the tomato SlPIF8b gene in regulating tomato fruit coloring and ripening, relating to the fields of genetic engineering and molecular biology. The tomato SlPIF8b gene has the nucleotide sequence shown in SEQ ID NO: 2. This application utilizes gene editing technology to knock out the SlPIF8b gene in tomatoes, which promotes the accumulation of lycopene and carotenoids in the tomato fruit, accelerates fruit coloring, and simultaneously promotes ethylene accumulation and softening, thus accelerating fruit ripening. This provides new germplasm resources for breeding new tomato varieties that promote fruit coloring and ripening, and has good potential application value. It also provides important theoretical basis and technical support for studying the molecular mechanisms of tomato ripening and improving the quality and nutritional value of tomato fruits.
Owner:SHENYANG AGRI UNIV

Application of PS gene in regulating tomato stem and leaf color, fruit color and maturity

PendingCN122404513AFruit maturationGenotype
This invention utilizes tomato gene tissue expression screening to obtain genes that are induced to be expressed during the ripening period of tomato fruits. PS ( Solyc10g084600 The gene encodes a protein containing a plant-specific TIGR01589 conserved domain of 57 amino acids. Furthermore, overexpression and gene editing technologies were used to... PS Overexpression and knockout of the gene coding region were performed, and genotypic and phenotypic identification were conducted on the overexpressed and mutant materials. Finally, it was determined that the gene has the biological function of negatively regulating chlorophyll accumulation and fruit ripening, and can be used for the breeding of tomato varieties with different ripening periods.
Owner:HUAZHONG AGRI UNIV +1

Method for improving germination rate of papaya seeds and cultivating high-quality seedlings

The invention belongs to the field of plant planting, and particularly relates to a method for improving the germination rate of papaya seeds and cultivating high-quality seedlings. Comprising the following steps: (1) seed selection: harvesting seeds on amphoteric plants when fruits are mature, taking out the seeds, removing aril, cleaning with clear water, airing, and selecting full seeds; (2) seed pretreatment; (3) peeling off testa; and (4) sowing the treated seeds in a seedling raising tray, and raising seedlings according to a conventional seedling cultivation method. According to the method, the germination rate of the papaya seeds is increased to 98.5% for the first time, the seedling time is shortened, germination and seedling emergence are neat, seedlings are thick and strong, root systems are developed, and the seedling quality is high. The method solves the problem of low germination rate or long germination time of papaya under conventional conditions, is an improvement of a papaya seedling culture key technology, and provides a simple and practical method for mass propagation of papaya seedlings. The method has the advantages of being simple in treatment process, low in cost, easy to operate and the like.
Owner:POMOLOGY RES INST FUJIAN ACAD OF AGRI SCI

MaSBP17 gene and application thereof in regulating and controlling fruit ripening

The invention provides an MaSBP17 gene and application thereof in regulating and controlling fruit maturity, and belongs to the technical field of gene engineering. The nucleotide sequence of the MaSBP17 gene is shown as SEQ ID NO: 1 in a sequence table. According to the invention, an SBP transcription factor family in a banana genome is comprehensively and systematically analyzed, a member MaSBP17 with significant expression change in a fruit ripening process is found, and the function of the MaSBP17 is verified by using a virus-induced gene silencing technology. The invention provides an important theoretical basis and a potential molecular target for targeted regulation and control of a banana fruit ripening process, delaying of softening and aging and development of a novel postharvest preservation strategy based on key transcription factors, and has remarkable scientific value and application prospect.
Owner:SOUTH SUBTROPICAL CROP RES INST CHINA ACAD OF TROPICAL AGRI SCI

Application of transcription factor CaABF3 in regulation and control of pepper fruit maturity

PendingCN120924577APlant peptidesFermentationBiotechnologyCarotene metabolism
The invention discloses application of a transcription factor CaABF3 in regulation and control of pepper fruit maturity. The invention relates to the technical field of plant genetic engineering, and provides any one of the following applications of CaABF3 protein: regulating and controlling ripening of plant fruits; the content of carotenoid in the plant fruits is regulated and controlled; the content of abscisic acid in the plants is regulated and controlled; the CaABF3 protein is a protein of which the amino acid sequence is SEQ ID NO: 1. A target fragment of the CaABF3 gene is amplified in pepper, a VIGS vector is constructed, and it is found that accumulation of carotenoids in pepper fruits can be reduced and fruit ripening can be delayed by inhibiting expression of the CaABF3 gene, so that the shelf life of the pepper is prolonged. Therefore, the CaABF3 gene plays an important role in metabolism of capsicum carotenoid, and has important significance on appearance and market value of fruits.
Owner:CHINA AGRI UNIV

Csadap gene and application thereof in regulating fruit ripening process

The application discloses a CsADAP gene and application thereof in regulating fruit ripening. The gene belongs to an AP2 / ERF family member, the CDS sequence of which is shown as SEQ ID NO. 1, the CDS sequence length is 1048 bp, and 348 amino acids are encoded, and the amino acid encoded by the gene is shown as SEQ ID NO. 2. A primer is designed to amplify the gene CsADAP on Fengjie navel orange, the gene is introduced into tomatoes and shanhuyan by using an agrobacterium-mediated genetic transformation method, and the obtained transgenic plants are verified by phenotype observation statistics and gene expression analysis, and it is shown that the CsADAP gene has the function of promoting fruit ripening, and provides a new gene resource for molecular design breeding of fruit ripening.
Owner:HUAZHONG AGRI UNIV

InDel molecular marker for distinguishing ziyang orange and japanese orange and application thereof

The application discloses an InDel molecular marker for distinguishing Ziyang orange and Japanese orange and application thereof, and a nucleotide sequence of the InDel molecular marker is shown as SEQ ID No. 1. The application obtains a kit for distinguishing Ziyang orange and Japanese orange, and the kit can be used to identify Ziyang orange and Japanese orange at a seedling stage, and can overcome problems of long period and low efficiency caused by dependence on field fruit phenotype observation in traditional methods. By using a seedling stage molecular marker diagnosis technology, seedling stage screening of Ziyang orange and Japanese orange can be realized, dependence on a fruit ripening season is broken, and a breeding period is significantly shortened. The kit is simple in operation, low in cost, and can be used for large-scale rapid screening and identification of Ziyang orange and Japanese orange.
Owner:HUAZHONG AGRI UNIV +3

Imidazole-type abscisic acid receptor antagonists and their use

The present invention relates to a novel type of compounds which modulate the activity of Abscisic Acid (ABA) receptors in plants. More particularly, such novel compounds are acting as ABA antagonists, which specifically and potently block activation of clade III ABA receptors including but not only PYL1, while another subfamily of compounds has agonistic mode of action promoting activation of the receptor and the ABA signaling pathway. These compounds are, therefore, applicable in different fields of modulating target plant seed germination, modulating target plant growth, modulating tolerance to drought and abiotic stress, modulating fruit ripening, or modulating immunity of a target plant. More particularly, said compounds can be used to stimulate the germination of plant seeds, either under conditions favorable for the growth of crop plants, or unfavorable for the survival of weeds. The compounds can also be used to enhance plant growth under growing conditions where water is not a limiting factor on plant growth. The selectivity of the compounds for clade III ABA receptors will prevent unwanted effects from blocking other ABA receptor subtypes (for example in clade II and clade I groups) present in the plants of interest. The present invention also provides corresponding agrochemical compositions comprising such novel compounds.
Owner:EURO LAB FUER MOLEKULARBIOLOGIE EMBL +2

A persimmon ripening device for food processing

The present application belongs to the technical field of fruit and vegetable processing, and particularly relates to a persimmon ripening device for food processing, which comprises a ripening box, an ethylene generating mechanism and an oxygen generating mechanism. The ethylene generating mechanism and the oxygen generating mechanism are both arranged on the ripening box and are both in communication with the ripening box. A plurality of placing plates for placing persimmons are slidably arranged in the ripening box at intervals. The ethylene generating mechanism comprises a hydraulic cavity, an air inlet pipeline, an air suction pipeline and a gas control assembly hole. The gas control assembly of the ethylene generating mechanism can control the ethylene content in the ripening box, so as to avoid that a part of the fruits ripen too fast or too slow and affect the overall fruit ripening effect. Meanwhile, the oxygen generating mechanism arranged can control the oxygen content in the ripening box, stimulate fruit respiration and improve the respiration efficiency to realize that the ripening degree of the final fruit is consistent, the water content and the sugar content of the fruit are improved, the taste of the fruit is improved, and the ripening purpose is achieved, and the work efficiency is improved.
Owner:GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI

Method for creating high-anthocyanin tomatoes through gene knock-in technology

The invention discloses a method for creating high-anthocyanin tomatoes through a gene knock-in technology. A homologous recombination mediated gene knock-in technology is utilized, the Aft gene of the tomato is knocked into the downstream of a promoter of a fruit ripening specific gene E8 at a fixed point, the E8 promoter is successfully utilized to activate Aft gene expression, and the high-anthocyanin tomato with purple black tomato fruit is obtained; and further performing selfing on the high-anthocyanin tomatoes, and screening out non-transgenic high-anthocyanin tomatoes which are subjected to Aft gene homozygous insertion and do not carry exogenous DNA fragments from selfing filial generations of the high-anthocyanin tomatoes. Therefore, the gene editing method disclosed by the invention can be used for quickly converting the common tomato without accumulation of anthocyanin into a high-nutrition tomato material rich in anthocyanin, and has great breeding application prospect and economic value.
Owner:SHANDONG AGRICULTURAL UNIVERSITY

Application of PavERF110 gene in regulating ripening direction of sweet cherry fruit

The application provides application of a PavERF110 gene in regulation of ripening direction of sweet cherry fruits, and belongs to the technical field of molecular breeding.The nucleotide sequence of the PavERF110 gene is shown as SEQ ID NO.1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO.2.The application also provides an overexpression vector and a VIGS gene silencing vector constructed from the gene and a construction method of the vectors, and recombinant microorganisms containing the vectors.It is found that overexpression of the PavERF110 gene can accelerate fruit color change and increase expression levels of mature-related genes by injecting and infecting engineering bacteria containing relevant vectors at the equator of fruits 25 days after flowering of the sweet cherry.The application provides new technical support and theoretical basis for improvement of fruit quality and regulation of ripening of the sweet cherry, helps to meet market demand, and deepens understanding of the transcriptional regulation mechanism of fruit ripening.
Owner:SICHUAN AGRI UNIV

Functional gene PaSPL8 for regulating and controlling maturity of apricot fruits and application of functional gene PaSPL8

The invention discloses a functional gene PaSPL8 for regulating and controlling maturity of apricot fruits. The nucleotide sequence of the gene PaSPL8 is as shown in SEQ ID NO. 1. The functional gene PaSPL8 for regulating and controlling apricot fruit ripening is used for regulating and controlling apricot or tomato fruit ripening, and fruit ripening is delayed by increasing the expression quantity of the gene PaSPL8; or the functional gene PaSPL8 for regulating and controlling the maturity of the apricot fruits is used for regulating and controlling the content of abscisic acid in plant tissues. The invention verifies that the PaSPL8 gene regulates and controls the maturity of apricot fruits by regulating and controlling the ABA content of apricot, so that the PaSPL8 gene can be used for apricot breeding.
Owner:RES INST OF NON TIMBER FORESTRY CHINESE ACAD OF FORESTRY

Large model auxiliary picking method and device and storage medium

The embodiment of the invention provides a large-model auxiliary picking method and device and a storage medium, and belongs to the field of intelligent agriculture and intelligent robot control. The large model auxiliary picking method comprises the steps that object parameters of a to-be-picked object and the equipment state and environment information of at least one picking robot are obtained, and the object parameters comprise at least one of the fruit maturity, the fruit position and the fruit health state; preprocessing the object parameter, the equipment state and the environment information, and then performing feature extraction and fusion to obtain a corresponding multi-dimensional state vector; taking the multi-dimensional state vector as the input of a large model, and outputting a picking strategy of each picking robot for the to-be-picked object through the large model; and controlling each picking robot to execute a corresponding picking task based on the picking strategy.
Owner:ZHONGSHAN POLYTECHNIC