58 results about "Sweet Cherries" patented technology

The invention provides an application of a PavMYB1 gene in regulation and control of sweet cherry anthocyanin synthesis. The nucleotide sequence of the PavMYB1 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the encoded protein of the PavMYB1 gene is as shown in SEQ ID NO. 2. The invention provides an overexpression vector and a VIGS gene silencing vector constructed by related genes, a construction method of the overexpression vector and the VIGS gene silencing vector, and a recombinant microorganism containing the vectors. Experiments prove that overexpression of PavMYB1 can increase fruit pigment accumulation and anthocyanin synthesis key gene expression, technical support and theoretical basis are provided for improvement of sweet cherry fruit quality, and revealing of a regulation mechanism of sweet cherry anthocyanin synthesis is facilitated.

The invention relates to the field of water-soluble fertilizers special for sweet cherries, and discloses a water-soluble fertilizer special for sweet cherries and a preparation and application method thereof.The water-soluble fertilizer special for sweet cherries comprises a drip irrigation fertilizer barrel A, a drip irrigation fertilizer barrel B, a drip irrigation fertilizer barrel C and a drip irrigation fertilizer barrel D, the drip irrigation fertilizer barrel C takes glycine, glutamic acid and glucose as cores to chelate metal ions; the fertilizer barrel A contains fulvic acid, nitrate nitrogen, potassium, molybdenum, zinc and a chelated iron element; the fertilizer barrel B contains ammonium polyphosphate, ammonium nitrogen, phosphorus, sulfur, manganese, boron and magnesium elements; the fertilizer C barrel comprises glycine, glutamic acid, glucose, amide nitrogen, nitrate nitrogen, calcium and copper. Compared with the prior art, the special water-soluble fertilizer disclosed by the invention has the advantages that formula design can be systematically and accurately carried out according to the growth cycle characteristics of the large cherries, and meanwhile, the problem of element antagonism is effectively solved.

The invention provides a foliage dressing method for promoting quality improvement and fruit cracking prevention of early cultivation of sweet cherries, and belongs to the technical field of sweet cherry planting management. Comprising the following steps: after autumn, ditching around sweet cherry trees and applying an organic fertilizer; a high-nitrogen macroelement water-soluble fertilizer is applied along with drip irrigation before and after germination; after flowers fall, in a young fruit period and in a fruit core hardening period, flushing calcium ammonium nitrate for 2-3 times; after flowers of the sweet cherries fall, foliar fertilizer is sprayed five times; applying potassium nitrate when the fruits turn from green to white; potassium sulfate is applied in the fruit changing from white to red; after the fruits are harvested, the balanced macroelement water-soluble fertilizer is applied by flushing for 1-2 times. According to the invention, a leaf fertilizer is set as an organic liquid chelated calcium fertilizer, a brand-new chelating technology and an acidification adsorption technology are combined, an organic chelating agent is taken as a carrier, and organic liquid chelated calcium prepared by an advanced process is adopted, so that fruits can rapidly absorb the organic liquid chelated calcium and can rapidly transfer the organic liquid chelated calcium in a plant body, and calcium contained in a part needing calcium is efficiently absorbed and utilized; the sugar can be effectively stored in fruit organs of plants.

They are prepared by a high energy method (e.g. by sonication) from: an oily solution of edible oils (e.g. high-oleic sunflower oil) as solvent, with melatonin (e.g. 100 μM) and edible fat-soluble antioxidant agents (e.g. 100 μM α-tocopherol), and an aqueous solution of edible water-soluble stabilizing agents (e.g. dextrin 1% w / v). These formulations, applied externally (e.g. by spraying) are useful for preharvest application and / or postharvest preservation of fruits or vegetables, particularly of stone fruits, and specifically of sweet cherries. In two varieties of sweet cherries and two varieties of plums, preservations with the formulations are significantly better than those reached with aqueous solutions of melatonin, or with a commercial formulation, or in the absence of treatment.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to the size of a sweet cherry fruit and application, and belongs to the technical field of genetic breeding of sweet cherries. The molecular marker disclosed by the invention is derived from an exon of FUN001609 and is positioned at a position 16366288 of a first chromosome of Prunus avium Tieton Genome v2.0, and nucleotide at the position is G or A. The molecular marker disclosed by the invention has the advantages that the molecular marker disclosed by the invention can be used for detecting the molecular marker; the SNP site is obviously related to the size of the sweet cherry fruit, and the genotype of the size of the sweet cherry fruit is AAgt; gGgt; gA. Meanwhile, a primer is designed according to the SNP site to carry out PCR amplification on the sweet cherries to be detected, and a PCR product is subjected to sequencing inspection. The SNP molecular marker disclosed by the invention can be used as an auxiliary selection marker of fruit size characters in a sweet cherry breeding process, so that the screening efficiency of large-fruit sweet cherry varieties is improved, the breeding period is shortened, and the breeding time and workload of the large-fruit varieties are greatly reduced.

This invention discloses a tea tree oil-thyme oil-chitosan composite film, its preparation method, and its application, belonging to the field of food preservation film technology. The preparation method of the tea tree oil-thyme oil-chitosan composite film includes the following steps: dissolving polyvinyl alcohol in water to obtain a polyvinyl alcohol solution; adding glacial acetic acid, chitosan, plasticizer, and emulsifier to the polyvinyl alcohol and mixing evenly; then adding tea tree oil and thyme oil, homogenizing and mixing, and degassing to obtain a film-forming liquid; and drying the film-forming liquid to form a film to obtain the tea tree oil-thyme oil-chitosan composite film. The tea tree oil-thyme oil-chitosan composite film prepared by this invention has excellent antibacterial effects and improves the preservation time of sweet cherries.

The invention discloses a PavSWEET9b gene for regulating and controlling the sugar content of sweet cherry fruits and application of the PavSWEET9b gene, and belongs to the technical field of gene engineering. The nucleotide sequence of the PavSWEET9b gene for regulating and controlling the sugar content of the sweet cherry fruits is shown as SEQ ID NO.1. The research finds that the PavSWEET9b protein is positioned on a cell membrane, and the soluble sugar content of the sweet cherry fruits is correspondingly reduced or increased after the gene is silenced and overexpressed, so that the PavSWEET9b gene can regulate and control the soluble sugar accumulation of the sweet cherry fruits; according to the method, the content of sugar in the sweet cherry fruits can be increased, so that the fruit quality is improved, the taste of the sweet cherry fruits is improved, the requirements of consumers are better met, and a new idea is provided for development of new sweet cherry varieties.

The invention discloses a molecular marker for identifying the cold demand of sweet cherries and application of the molecular marker. The nucleotide sequence of the molecular marker is shown as SEQ ID NO: 1; the molecular marker corresponds to large fragment deletion sites at 1, 589 and 063bp of a sweet cherry chromosome 2, 655bp deletion polymorphism begins to exist at the 623th nucleotide, and the polymorphism site is associated with the cold demand of sweet cherries; the nucleotide sequence corresponding to the 655bp deletion is shown as SEQ ID NO.4. The molecular marker disclosed by the invention can be used as a molecular identity card of a low-cold-demand sweet cherry variety, a reliable and scientific basis is provided for registration and authentication of a new variety and intellectual property protection, and variety confusion and infringement are effectively prevented; meanwhile, seedling quality control is assisted, it is ensured that the planted variety is consistent with the target cold demand type, the sweet cherry industry is further promoted to develop towards the standardization and normalization direction, and the industrial economic value is guaranteed.

The invention discloses a tissue culture rooting medium and application thereof in sweet cherry genetic transformation, and relates to the technical field of sweet cherry tissue culture. The sweet cherry tissue culture rooting culture medium is moss soaked in a rooting culture solution, the rooting culture solution comprises 4.43 g / L of an MS culture medium, 30 g / L of cane sugar, 0.5 mg / L of IBA and 0.2 mg / L of NAA, and the pH is 5.8. When the rooting culture medium is used for a sweet cherry genetic transformation experiment, tissue culture seedlings are not easy to pollute, the rooting rate and the positive seedling obtaining rate are remarkably improved, the seedling death rate in the culture process is remarkably reduced, the operation is simplified, the cost is saved, and the sweet cherry genetic transformation efficiency is greatly improved.

The present invention discloses AFLP molecular markers, detection primers, and applications for sweet cherry fruit firmness. Using BSA-seq, the present invention preliminarily locates the target gene segment and performs fine mapping, determining that the key gene controlling sweet cherry fruit firmness is located within the 473Kb interval on chromosome 6. Combining gene expression analysis and gene cloning, the present invention identifies the gene PavSCPL controlling sweet cherry fruit firmness, and that a 5.2Kb transposon insertion allele is present in the coding region of the PavSCPL gene for firm-fleshed sweet cherries. This 5.2Kb transposon insertion allele inactivates the PavSCPL gene. Based on this, the present invention develops an AFLP molecular marker for sweet cherry fruit firmness. The AFLP molecular marker achieves 100% accuracy in detecting sweet cherry fruit firmness, demonstrating promising applications in assisting the genetic improvement of the firm flesh trait in sweet cherries and in the selection of storability-oriented sweet cherry germplasm.

The invention relates to a sweet cherry fruit hardness related molecular marker Marker 2916 and application thereof. According to the application, a hard-fruit sweet cherry variety 'Yanhongzhu' is taken as a female parent, and a soft-fruit sweet cherry variety 'Shangnan 2808' is taken as a male parent, so that a genetic population with separated hardness and softness is constructed. By means of QTL positioning, the fruit hardness major QTL is positioned on an LG4 chromosome for three consecutive years, the QTL is named as qFF4.1, a molecular marker Marker2916 is found near the qFF4.1, and when the genotype is A / A, the sweet cherry fruit is a soft fruit; when the genotype is C / C, the sweet cherry fruits are hard fruits. The development of the KASP marker reduces the detection cost of the SNP, and by identifying and screening fruit hardness genotypes of filial generations of different sweet cherry varieties, the breeding efficiency can be obviously improved, and the breeding cost can be saved.

The present application relates to cherry molecular marker assisted germplasm identification and breeding technical field, specifically to a kind of InDel marker for identifying Chinese cherry and European sweet cherry germplasm and its application, suitable for identifying cherry resources of Chinese cherry and European sweet cherry two species and interspecific hybrid thereof.The present application designs specific PCR primer using InDel sequence information of cherry genome Cp2_35932912 site, extracts Chinese cherry and European sweet cherry genomic DNA, carries out PCR amplification and polyacrylamide gel electrophoresis, reads electrophoresis band, identifies Chinese cherry, European sweet cherry and interspecific hybrid thereof according to band position and number, develops InDel molecular marker, which can be used for Chinese cherry and European sweet cherry germplasm identification, and also can be used for early identification of interspecific hybridization offspring hybrid authenticity, and the result is accurate and reliable.

The application provides application of a PavERF110 gene in regulation of ripening direction of sweet cherry fruits, and belongs to the technical field of molecular breeding.The nucleotide sequence of the PavERF110 gene is shown as SEQ ID NO.1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO.2.The application also provides an overexpression vector and a VIGS gene silencing vector constructed from the gene and a construction method of the vectors, and recombinant microorganisms containing the vectors.It is found that overexpression of the PavERF110 gene can accelerate fruit color change and increase expression levels of mature-related genes by injecting and infecting engineering bacteria containing relevant vectors at the equator of fruits 25 days after flowering of the sweet cherry.The application provides new technical support and theoretical basis for improvement of fruit quality and regulation of ripening of the sweet cherry, helps to meet market demand, and deepens understanding of the transcriptional regulation mechanism of fruit ripening.

1. Name of the designed product: daily chemical packaging bottle (clothes softener - sweet cherry blossom). 2. Use of the designed product: the designed product is used as a packaging bottle for softener, also known as clothes care agent. 3. Design points of the designed product: the combination of shape and pattern. 4. Picture or photo best indicating the design points: front view.

1. Name of the product in this design: Core remover. 2. Purpose of this design: For pitting cherries, sweet cherries, dates, and tangerines. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The present invention relates to a method for cultivating sweet cherries in southern China, and more particularly to a two-year, three-season off-season cultivation method for sweet cherries in southern China. The method comprises selecting a high-temperature-resistant sweet cherry variety suitable for cultivation in southern China, and utilizing an intelligent artificial environment control unit to regulate the temperature, humidity, and light intensity within a cultivation greenhouse, thereby forming a two-year, three-season cultivation cycle. The specific steps comprise: controlling the temperature within the greenhouse at 2-4°C during the dormant period of each cultivation cycle, without light, to ensure that the tree enters dormancy; gradually increasing the temperature to 10-20°C during the budding period; maintaining the greenhouse temperature at 12-25°C during the flowering period; maintaining the greenhouse temperature at 15-25°C during the young fruiting period by using refrigeration equipment and supplementary lighting, and ensuring 15-18 hours of light per day; and controlling the greenhouse temperature at 18-28°C during the ripening period. The present invention can significantly improve the yield and quality of sweet cherries, adapt to the needs of off-season cultivation in southern China, and has broad application prospects.

The invention relates to the field of cultivation of European sweet cherries, and discloses an auxiliary cultivation frame for European sweet cherries and a tree-shaped cultivation method of the auxiliary cultivation frame for European sweet cherries. The steel frame shaped like the Chinese character'feng 'comprises a center vertical steel pipe, and a first steel pipe, a second steel pipe and a third steel pipe which are parallel to the ground are fixedly connected to the center vertical steel pipe in the height direction. And a first layer of steel wires, a second layer of steel wires, a third layer of steel wires and a fourth layer of steel wires are fixedly connected among the adjacent central upright steel pipes, the first steel pipes, the second steel pipes and the third steel pipes from bottom to top. Compared with the prior art, the method has the advantages that datamation and standardization operation can be achieved in the whole process, the problem that fruit tree management is difficult to standard is truly solved, the consistency of the sweet cherry fruit quality is completed on the basis of standardization, and high commodity value is achieved.

This utility model discloses a branch traction structure for sweet cherry planting, relating to the field of cherry tree planting technology. It includes a fixed base, a rotating seat mounted on the upper surface of the fixed base, a telescopic cylinder rotatably mounted on the inner surface of the rotating seat, and an extension rod slidably mounted on the upper surface of the telescopic cylinder. Compared with existing ordinary cherry tree branch pulling devices, this branch traction structure for sweet cherry planting, through the setting of a tension spring, can buffer the tension on the branch when the branch is fixed using a traction frame, especially in strong winds, by stretching the tension spring, thus preventing damage to the branch due to excessive pulling force. The threaded groove allows control of the rotating pressure sleeve to move along the threaded groove, adjusting the tension threshold of the tension spring, ensuring that the branch is stretched and decompressed only after receiving a certain amount of tension, preventing slight tension from causing the branch to sway and affecting the traction effect.

A new and distinct cultivar of Prunus rootstock tree named ‘RVL-4’ is disclosed, characterized by high precocity and mildew resistance. ‘RVL-4’ is suitable for use as a rootstock upon which commercial fruit bearing sweet cherry varieties may be budded or grafted. The new variety is a Prunus vulgaris x maacki x lannesiana, typically produced for rootstock.

The present invention discloses a temperature and humidity intelligent two-way regulation facility and equipment for sweet cherries, including a frame body. An organism is arranged inside the frame body. Rotating shafts are installed on the top wall and the bottom wall inside the frame body. A frame door is fixedly installed between the ends of the two rotating shafts close to each other. A limiting component is arranged on the surface of the frame body, and the limiting component is used to limit the frame door. The limiting component includes a mounting seat and a limiting plate. A plug is fixedly installed on one side wall of the frame door, and a mounting opening for the plug to insert is formed on one side wall of the frame body. The mounting seat is fixedly installed on the top wall of the mounting opening, and a magnetic strip is fixedly installed on the bottom wall of the mounting opening. The limiting plate is made of magnetic material. Through the cooperation between the limiting component and the magnetic strip, the present invention can quickly open and close the frame door, avoiding the cumbersome steps of using screw and nut fixing parts, and facilitating the later maintenance and repair of the device main body inside the frame body by the staff.

The invention relates to the technical field of molecular marker-assisted germplasm resource identification and breeding of cherries, in particular to an InDel marker for identifying germplasm of Chinese cherries and European sweet cherries and application of the InDel marker, which are suitable for identifying two species of the Chinese cherries and the European sweet cherries and interspecific hybrid cherry resources of the two species of the Chinese cherries and the European sweet cherries. The InDel molecular marker is developed by designing a specific PCR (Polymerase Chain Reaction) primer by utilizing InDel sequence information of a cherry genome Cp235932912 site, extracting genomic DNA (Deoxyribose Nucleic Acid) of Chinese cherries and European sweet cherries, performing PCR amplification and polyacrylamide gel electrophoresis, reading electrophoretic bands, identifying the Chinese cherries, the European sweet cherries and interspecific hybrids thereof according to the positions and the number of the bands, and identifying the Chinese cherries, the European sweet cherries and the interspecific hybrids of the Chinese cherries, the European sweet cherries and the European sweet cherries according to the InDel molecular marker. The molecular marker can be used for germplasm identification of Chinese cherries and European sweet cherries, and can also be used for early identification of hybrid authenticity of interspecific filial generations of the Chinese cherries and the European sweet cherries, and results are accurate and reliable.

The invention relates to a molecular marker Marker 2937 related to the fruit hardness of sweet cherries and application of the molecular marker Marker 2937. The amplification result of the three KSASP primers designed by the invention is that the 16080735bp position of the chromosome 4 of the sweet cherry genome is an SNP site, the genotype of the SNP site is defined as T / T in a hard fruit material, and the genotype of the SNP site is defined as A / A in a soft fruit material. The amplification effect can obviously and effectively distinguish the genotype of the SNP site in the sweet cherry fruit, the nucleotide mutation site can be rapidly and accurately detected, and an accurate, rapid and effective method is provided for breeding of a hard sweet cherry variety. The development of the KASP marker can improve the identification and screening of fruit hardness genotypes of filial generations of different sweet cherry varieties, a phenotype identification method is not adopted, the breeding efficiency can be obviously improved, and the breeding cost is saved.

The application provides application of PavMYB1 gene in regulation of anthocyanin synthesis of sweet cherry. The nucleotide sequence of the PavMYB1 gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. The application provides an overexpression vector and a VIGS gene silencing vector constructed by related genes and a construction method thereof, and a recombinant microorganism containing the vectors. The application proves through experiments that overexpression of PavMYB1 can increase pigment accumulation and expression of key anthocyanin synthesis genes of fruits, provides technical support and theoretical basis for improvement of fruit quality of sweet cherry, and helps to reveal the regulation mechanism of anthocyanin synthesis of sweet cherry.

The utility model provides a simple cultivation facility for preventing malformed fruits of sweet cherries, which belongs to the technical field of fruit tree cultivation, and comprises three main beam frames, the main beam frames are triangular, cross beams are fixedly connected among the upper ends of the three main beam frames, the lower surfaces of the main beam frames are fixedly connected with supporting rods, and the supporting rods are fixedly connected with the main beam frames. A connecting structure is arranged between every two adjacent main beam frames, four folding assemblies are arranged on the upper side faces of the main beam frames, a power structure is arranged on the upper surface of each cross beam, each connecting structure comprises a main connecting column and an auxiliary connecting column, and the main connecting columns and the auxiliary connecting columns are fixedly connected between the adjacent main beam frames. The folding structure and the power structure are arranged, when sunlight illumination is too strong, the steel rope can be released through the transmission machine, the main folding plate, the secondary folding plate and the tail folding plate can be lowered to the position above the main beam frame, then the sunlight is shielded to a certain degree, and influences on cherries are avoided.

The present invention relates to the technical field of protected agriculture. The present invention provides a method for ultra-high density cultivation of sweet cherry with root restriction in substrate bags, comprising the following steps: (1) In the first year after seedling planting, pinch the lateral bud shoots; in winter, carry out pruning; (2) In the second year, carry out bud thinning after the dormancy of the apical shoots begins to germinate; pinch the lateral branches; in winter, carry out pruning; (3) In the third year, carry out bud thinning after the dormancy of the apical shoots begins to germinate; pinch the lateral branches; in winter, carry out pruning. The cultivation method of the present invention improves the tree shape to obtain an ultra-thin spindle tree shape; restricts the roots through breathable substrate bags, effectively controls the vegetative growth of the tree body, promotes flower bud differentiation and fruit development, improves the yield and quality of fruits, and then uses the integrated water and fertilizer technology to reduce fertilizer waste and environmental pollution, realizing green production.

The application provides application of PavDREB1 gene in regulation of aging direction of sweet cherry fruits, and belongs to the technical field of molecular breeding.The nucleotide sequence of the PavDREB1 gene is shown as SEQ ID NO.1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO.2.The application also provides an overexpression vector and a VIGS gene silencing gene vector constructed from the gene and a construction method of the vector, and recombinant microorganisms containing the vector.The application finds that overexpression of the PavDREB1 gene can accelerate fruit aging and increase the expression level of aging-related genes by injecting the engineering bacteria containing the related vector into the equator of the fruits 25 days after flowering of the sweet cherry, and silencing the gene can slow down fruit aging and the expression of related genes.The application provides a new molecular assisted breeding strategy and theoretical basis for postharvest preservation of sweet cherry fruits.

The invention discloses a rain sheltering device for preventing fruit cracking of sweet cherries, and belongs to the technical field of rain sheltering devices.The rain sheltering device comprises two base plates which are arranged in a mirror symmetry mode, the tops of the base plates are slidably connected with a plurality of moving frames which are distributed in a linear array mode, and folding and unfolding assemblies are arranged at the bottoms of the multiple moving frames. According to the device, by arranging the stabilizing assembly, a worker drives a supporting barbed nail to move towards one side and go deep into the ground through a rotating shaft, a rotating shaft, a rotating rod, a sleeve, a first bevel gear, a second bevel gear, a movable lead screw and a fixed sliding rod, so that the moving stroke of the supporting barbed nail is changed, and the supporting barbed nail is obliquely inserted into the ground in an inclined mode; and inclined support is provided for the movable frame and the base plate, so that the stability of the device is improved, the anti-impact capability and the anti-toppling capability of the device facing wind and rain are improved, the service life of the device is prolonged, and the influence of rainwater on cherries is reduced.

The invention relates to a method for regulating and controlling the storage quality of sweet cherry fruits through cooperation of a low-voltage electrostatic field and melatonin under plasma pretreatment, and belongs to the technical field of fruit preservation. The regulation and control method comprises the following steps: S1, pretreatment: performing plasma treatment on sweet cherries; s2, melatonin treatment: dissolving melatonin powder in ultrapure water to prepare a fresh-keeping solution, then putting the pretreated sweet cherry fruits in the fresh-keeping solution for soaking treatment, and then draining on a sterile filter screen; and S3, low-voltage electrostatic field treatment: putting the sweet cherry fruits treated by melatonin into a low-voltage electrostatic field, and carrying out low-voltage electrostatic field treatment. On the basis of low-temperature normal-pressure plasma (CAP) pretreatment creatively, a low-voltage electrostatic field (LVEF) and exogenous melatonin (MT) are cooperatively applied, and the regulation and control effect of the composite treatment on the fruit storage quality is systematically evaluated.

The invention provides a facility delayed cultivation method for sweet cherries, and belongs to the technical field of fruit tree cultivation. The facility delayed cultivation method for the sweet cherries provided by the invention comprises the following steps: cultivating middle and late maturing sweet cherry varieties in a sunlight greenhouse; nutrients are increased and hormone control is performed from early October to leaf falling every year; covering the greenhouse for heat preservation in late October; compared with cherry trees cultivated in the open field, the method delays leaf falling for 5-10 days and then promotes nutrient backflow; after leaves fall, the dormancy period is delayed through temperature control; performing temperature and humidity control, hormone treatment and frozen soil layer control in a dormancy period to prolong the dormancy period; performing temperature and humidity control from germination to the initial stage of flowering, and performing pollination while performing temperature and humidity control in the flowering stage; performing temperature and humidity control and hormone treatment from flower falling to a hard core stage, gradually adapting to the outdoor environment after entering the hard core stage, removing the greenhouse film, and entering an open field management stage. By delaying the dormancy starting time and prolonging the dormancy period, delayed ripening of the sweet cherries is achieved, early frost damage can be effectively avoided, and the economic benefits of an orchard are improved.