47 results about "Sweet Cherries" patented technology

They are prepared by a high energy method (e.g. by sonication) from: an oily solution of edible oils (e.g. high-oleic sunflower oil) as solvent, with melatonin (e.g. 100 μM) and edible fat-soluble antioxidant agents (e.g. 100 μM α-tocopherol), and an aqueous solution of edible water-soluble stabilizing agents (e.g. dextrin 1% w / v). These formulations, applied externally (e.g. by spraying) are useful for preharvest application and / or postharvest preservation of fruits or vegetables, particularly of stone fruits, and specifically of sweet cherries. In two varieties of sweet cherries and two varieties of plums, preservations with the formulations are significantly better than those reached with aqueous solutions of melatonin, or with a commercial formulation, or in the absence of treatment.

This invention discloses a tea tree oil-thyme oil-chitosan composite film, its preparation method, and its application, belonging to the field of food preservation film technology. The preparation method of the tea tree oil-thyme oil-chitosan composite film includes the following steps: dissolving polyvinyl alcohol in water to obtain a polyvinyl alcohol solution; adding glacial acetic acid, chitosan, plasticizer, and emulsifier to the polyvinyl alcohol and mixing evenly; then adding tea tree oil and thyme oil, homogenizing and mixing, and degassing to obtain a film-forming liquid; and drying the film-forming liquid to form a film to obtain the tea tree oil-thyme oil-chitosan composite film. The tea tree oil-thyme oil-chitosan composite film prepared by this invention has excellent antibacterial effects and improves the preservation time of sweet cherries.

The invention discloses a PavSWEET9b gene for regulating and controlling the sugar content of sweet cherry fruits and application of the PavSWEET9b gene, and belongs to the technical field of gene engineering. The nucleotide sequence of the PavSWEET9b gene for regulating and controlling the sugar content of the sweet cherry fruits is shown as SEQ ID NO.1. The research finds that the PavSWEET9b protein is positioned on a cell membrane, and the soluble sugar content of the sweet cherry fruits is correspondingly reduced or increased after the gene is silenced and overexpressed, so that the PavSWEET9b gene can regulate and control the soluble sugar accumulation of the sweet cherry fruits; according to the method, the content of sugar in the sweet cherry fruits can be increased, so that the fruit quality is improved, the taste of the sweet cherry fruits is improved, the requirements of consumers are better met, and a new idea is provided for development of new sweet cherry varieties.

The invention discloses a molecular marker for identifying the cold demand of sweet cherries and application of the molecular marker. The nucleotide sequence of the molecular marker is shown as SEQ ID NO: 1; the molecular marker corresponds to large fragment deletion sites at 1, 589 and 063bp of a sweet cherry chromosome 2, 655bp deletion polymorphism begins to exist at the 623th nucleotide, and the polymorphism site is associated with the cold demand of sweet cherries; the nucleotide sequence corresponding to the 655bp deletion is shown as SEQ ID NO.4. The molecular marker disclosed by the invention can be used as a molecular identity card of a low-cold-demand sweet cherry variety, a reliable and scientific basis is provided for registration and authentication of a new variety and intellectual property protection, and variety confusion and infringement are effectively prevented; meanwhile, seedling quality control is assisted, it is ensured that the planted variety is consistent with the target cold demand type, the sweet cherry industry is further promoted to develop towards the standardization and normalization direction, and the industrial economic value is guaranteed.

The invention discloses a tissue culture rooting medium and application thereof in sweet cherry genetic transformation, and relates to the technical field of sweet cherry tissue culture. The sweet cherry tissue culture rooting culture medium is moss soaked in a rooting culture solution, the rooting culture solution comprises 4.43 g / L of an MS culture medium, 30 g / L of cane sugar, 0.5 mg / L of IBA and 0.2 mg / L of NAA, and the pH is 5.8. When the rooting culture medium is used for a sweet cherry genetic transformation experiment, tissue culture seedlings are not easy to pollute, the rooting rate and the positive seedling obtaining rate are remarkably improved, the seedling death rate in the culture process is remarkably reduced, the operation is simplified, the cost is saved, and the sweet cherry genetic transformation efficiency is greatly improved.

The present invention discloses AFLP molecular markers, detection primers, and applications for sweet cherry fruit firmness. Using BSA-seq, the present invention preliminarily locates the target gene segment and performs fine mapping, determining that the key gene controlling sweet cherry fruit firmness is located within the 473Kb interval on chromosome 6. Combining gene expression analysis and gene cloning, the present invention identifies the gene PavSCPL controlling sweet cherry fruit firmness, and that a 5.2Kb transposon insertion allele is present in the coding region of the PavSCPL gene for firm-fleshed sweet cherries. This 5.2Kb transposon insertion allele inactivates the PavSCPL gene. Based on this, the present invention develops an AFLP molecular marker for sweet cherry fruit firmness. The AFLP molecular marker achieves 100% accuracy in detecting sweet cherry fruit firmness, demonstrating promising applications in assisting the genetic improvement of the firm flesh trait in sweet cherries and in the selection of storability-oriented sweet cherry germplasm.

The invention relates to a sweet cherry fruit hardness related molecular marker Marker 2916 and application thereof. According to the application, a hard-fruit sweet cherry variety 'Yanhongzhu' is taken as a female parent, and a soft-fruit sweet cherry variety 'Shangnan 2808' is taken as a male parent, so that a genetic population with separated hardness and softness is constructed. By means of QTL positioning, the fruit hardness major QTL is positioned on an LG4 chromosome for three consecutive years, the QTL is named as qFF4.1, a molecular marker Marker2916 is found near the qFF4.1, and when the genotype is A / A, the sweet cherry fruit is a soft fruit; when the genotype is C / C, the sweet cherry fruits are hard fruits. The development of the KASP marker reduces the detection cost of the SNP, and by identifying and screening fruit hardness genotypes of filial generations of different sweet cherry varieties, the breeding efficiency can be obviously improved, and the breeding cost can be saved.

The present application relates to cherry molecular marker assisted germplasm identification and breeding technical field, specifically to a kind of InDel marker for identifying Chinese cherry and European sweet cherry germplasm and its application, suitable for identifying cherry resources of Chinese cherry and European sweet cherry two species and interspecific hybrid thereof.The present application designs specific PCR primer using InDel sequence information of cherry genome Cp2_35932912 site, extracts Chinese cherry and European sweet cherry genomic DNA, carries out PCR amplification and polyacrylamide gel electrophoresis, reads electrophoresis band, identifies Chinese cherry, European sweet cherry and interspecific hybrid thereof according to band position and number, develops InDel molecular marker, which can be used for Chinese cherry and European sweet cherry germplasm identification, and also can be used for early identification of interspecific hybridization offspring hybrid authenticity, and the result is accurate and reliable.

The application provides application of a PavERF110 gene in regulation of ripening direction of sweet cherry fruits, and belongs to the technical field of molecular breeding.The nucleotide sequence of the PavERF110 gene is shown as SEQ ID NO.1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO.2.The application also provides an overexpression vector and a VIGS gene silencing vector constructed from the gene and a construction method of the vectors, and recombinant microorganisms containing the vectors.It is found that overexpression of the PavERF110 gene can accelerate fruit color change and increase expression levels of mature-related genes by injecting and infecting engineering bacteria containing relevant vectors at the equator of fruits 25 days after flowering of the sweet cherry.The application provides new technical support and theoretical basis for improvement of fruit quality and regulation of ripening of the sweet cherry, helps to meet market demand, and deepens understanding of the transcriptional regulation mechanism of fruit ripening.

1. Name of the designed product: daily chemical packaging bottle (clothes softener - sweet cherry blossom). 2. Use of the designed product: the designed product is used as a packaging bottle for softener, also known as clothes care agent. 3. Design points of the designed product: the combination of shape and pattern. 4. Picture or photo best indicating the design points: front view.

1. Name of the product in this design: Core remover. 2. Purpose of this design: For pitting cherries, sweet cherries, dates, and tangerines. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The present invention relates to a method for cultivating sweet cherries in southern China, and more particularly to a two-year, three-season off-season cultivation method for sweet cherries in southern China. The method comprises selecting a high-temperature-resistant sweet cherry variety suitable for cultivation in southern China, and utilizing an intelligent artificial environment control unit to regulate the temperature, humidity, and light intensity within a cultivation greenhouse, thereby forming a two-year, three-season cultivation cycle. The specific steps comprise: controlling the temperature within the greenhouse at 2-4°C during the dormant period of each cultivation cycle, without light, to ensure that the tree enters dormancy; gradually increasing the temperature to 10-20°C during the budding period; maintaining the greenhouse temperature at 12-25°C during the flowering period; maintaining the greenhouse temperature at 15-25°C during the young fruiting period by using refrigeration equipment and supplementary lighting, and ensuring 15-18 hours of light per day; and controlling the greenhouse temperature at 18-28°C during the ripening period. The present invention can significantly improve the yield and quality of sweet cherries, adapt to the needs of off-season cultivation in southern China, and has broad application prospects.

This utility model discloses a branch traction structure for sweet cherry planting, relating to the field of cherry tree planting technology. It includes a fixed base, a rotating seat mounted on the upper surface of the fixed base, a telescopic cylinder rotatably mounted on the inner surface of the rotating seat, and an extension rod slidably mounted on the upper surface of the telescopic cylinder. Compared with existing ordinary cherry tree branch pulling devices, this branch traction structure for sweet cherry planting, through the setting of a tension spring, can buffer the tension on the branch when the branch is fixed using a traction frame, especially in strong winds, by stretching the tension spring, thus preventing damage to the branch due to excessive pulling force. The threaded groove allows control of the rotating pressure sleeve to move along the threaded groove, adjusting the tension threshold of the tension spring, ensuring that the branch is stretched and decompressed only after receiving a certain amount of tension, preventing slight tension from causing the branch to sway and affecting the traction effect.

A new and distinct cultivar of Prunus rootstock tree named ‘RVL-4’ is disclosed, characterized by high precocity and mildew resistance. ‘RVL-4’ is suitable for use as a rootstock upon which commercial fruit bearing sweet cherry varieties may be budded or grafted. The new variety is a Prunus vulgaris x maacki x lannesiana, typically produced for rootstock.

The present invention discloses a temperature and humidity intelligent two-way regulation facility and equipment for sweet cherries, including a frame body. An organism is arranged inside the frame body. Rotating shafts are installed on the top wall and the bottom wall inside the frame body. A frame door is fixedly installed between the ends of the two rotating shafts close to each other. A limiting component is arranged on the surface of the frame body, and the limiting component is used to limit the frame door. The limiting component includes a mounting seat and a limiting plate. A plug is fixedly installed on one side wall of the frame door, and a mounting opening for the plug to insert is formed on one side wall of the frame body. The mounting seat is fixedly installed on the top wall of the mounting opening, and a magnetic strip is fixedly installed on the bottom wall of the mounting opening. The limiting plate is made of magnetic material. Through the cooperation between the limiting component and the magnetic strip, the present invention can quickly open and close the frame door, avoiding the cumbersome steps of using screw and nut fixing parts, and facilitating the later maintenance and repair of the device main body inside the frame body by the staff.

The invention relates to a molecular marker Marker 2937 related to the fruit hardness of sweet cherries and application of the molecular marker Marker 2937. The amplification result of the three KSASP primers designed by the invention is that the 16080735bp position of the chromosome 4 of the sweet cherry genome is an SNP site, the genotype of the SNP site is defined as T / T in a hard fruit material, and the genotype of the SNP site is defined as A / A in a soft fruit material. The amplification effect can obviously and effectively distinguish the genotype of the SNP site in the sweet cherry fruit, the nucleotide mutation site can be rapidly and accurately detected, and an accurate, rapid and effective method is provided for breeding of a hard sweet cherry variety. The development of the KASP marker can improve the identification and screening of fruit hardness genotypes of filial generations of different sweet cherry varieties, a phenotype identification method is not adopted, the breeding efficiency can be obviously improved, and the breeding cost is saved.

The application provides application of PavMYB1 gene in regulation of anthocyanin synthesis of sweet cherry. The nucleotide sequence of the PavMYB1 gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. The application provides an overexpression vector and a VIGS gene silencing vector constructed by related genes and a construction method thereof, and a recombinant microorganism containing the vectors. The application proves through experiments that overexpression of PavMYB1 can increase pigment accumulation and expression of key anthocyanin synthesis genes of fruits, provides technical support and theoretical basis for improvement of fruit quality of sweet cherry, and helps to reveal the regulation mechanism of anthocyanin synthesis of sweet cherry.

The utility model provides a simple cultivation facility for preventing malformed fruits of sweet cherries, which belongs to the technical field of fruit tree cultivation, and comprises three main beam frames, the main beam frames are triangular, cross beams are fixedly connected among the upper ends of the three main beam frames, the lower surfaces of the main beam frames are fixedly connected with supporting rods, and the supporting rods are fixedly connected with the main beam frames. A connecting structure is arranged between every two adjacent main beam frames, four folding assemblies are arranged on the upper side faces of the main beam frames, a power structure is arranged on the upper surface of each cross beam, each connecting structure comprises a main connecting column and an auxiliary connecting column, and the main connecting columns and the auxiliary connecting columns are fixedly connected between the adjacent main beam frames. The folding structure and the power structure are arranged, when sunlight illumination is too strong, the steel rope can be released through the transmission machine, the main folding plate, the secondary folding plate and the tail folding plate can be lowered to the position above the main beam frame, then the sunlight is shielded to a certain degree, and influences on cherries are avoided.

The present invention relates to the technical field of protected agriculture. The present invention provides a method for ultra-high density cultivation of sweet cherry with root restriction in substrate bags, comprising the following steps: (1) In the first year after seedling planting, pinch the lateral bud shoots; in winter, carry out pruning; (2) In the second year, carry out bud thinning after the dormancy of the apical shoots begins to germinate; pinch the lateral branches; in winter, carry out pruning; (3) In the third year, carry out bud thinning after the dormancy of the apical shoots begins to germinate; pinch the lateral branches; in winter, carry out pruning. The cultivation method of the present invention improves the tree shape to obtain an ultra-thin spindle tree shape; restricts the roots through breathable substrate bags, effectively controls the vegetative growth of the tree body, promotes flower bud differentiation and fruit development, improves the yield and quality of fruits, and then uses the integrated water and fertilizer technology to reduce fertilizer waste and environmental pollution, realizing green production.

The application provides application of PavDREB1 gene in regulation of aging direction of sweet cherry fruits, and belongs to the technical field of molecular breeding.The nucleotide sequence of the PavDREB1 gene is shown as SEQ ID NO.1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO.2.The application also provides an overexpression vector and a VIGS gene silencing gene vector constructed from the gene and a construction method of the vector, and recombinant microorganisms containing the vector.The application finds that overexpression of the PavDREB1 gene can accelerate fruit aging and increase the expression level of aging-related genes by injecting the engineering bacteria containing the related vector into the equator of the fruits 25 days after flowering of the sweet cherry, and silencing the gene can slow down fruit aging and the expression of related genes.The application provides a new molecular assisted breeding strategy and theoretical basis for postharvest preservation of sweet cherry fruits.

The invention relates to a method for regulating and controlling the storage quality of sweet cherry fruits through cooperation of a low-voltage electrostatic field and melatonin under plasma pretreatment, and belongs to the technical field of fruit preservation. The regulation and control method comprises the following steps: S1, pretreatment: performing plasma treatment on sweet cherries; s2, melatonin treatment: dissolving melatonin powder in ultrapure water to prepare a fresh-keeping solution, then putting the pretreated sweet cherry fruits in the fresh-keeping solution for soaking treatment, and then draining on a sterile filter screen; and S3, low-voltage electrostatic field treatment: putting the sweet cherry fruits treated by melatonin into a low-voltage electrostatic field, and carrying out low-voltage electrostatic field treatment. On the basis of low-temperature normal-pressure plasma (CAP) pretreatment creatively, a low-voltage electrostatic field (LVEF) and exogenous melatonin (MT) are cooperatively applied, and the regulation and control effect of the composite treatment on the fruit storage quality is systematically evaluated.

The invention provides a facility delayed cultivation method for sweet cherries, and belongs to the technical field of fruit tree cultivation. The facility delayed cultivation method for the sweet cherries provided by the invention comprises the following steps: cultivating middle and late maturing sweet cherry varieties in a sunlight greenhouse; nutrients are increased and hormone control is performed from early October to leaf falling every year; covering the greenhouse for heat preservation in late October; compared with cherry trees cultivated in the open field, the method delays leaf falling for 5-10 days and then promotes nutrient backflow; after leaves fall, the dormancy period is delayed through temperature control; performing temperature and humidity control, hormone treatment and frozen soil layer control in a dormancy period to prolong the dormancy period; performing temperature and humidity control from germination to the initial stage of flowering, and performing pollination while performing temperature and humidity control in the flowering stage; performing temperature and humidity control and hormone treatment from flower falling to a hard core stage, gradually adapting to the outdoor environment after entering the hard core stage, removing the greenhouse film, and entering an open field management stage. By delaying the dormancy starting time and prolonging the dormancy period, delayed ripening of the sweet cherries is achieved, early frost damage can be effectively avoided, and the economic benefits of an orchard are improved.

The present application relates to the technical field of fruit tree planting, and particularly relates to a method for evaluating the high-yield property of sweet cherry. The present application provides a method for evaluating the high-yield property of sweet cherry, which comprises the following steps: grafting the sweet cherry scion to be evaluated onto a rootstock, waiting for the scion to develop to the flower separation period, counting the proportion of the number of flower buds containing 2-4 flowers in the total number of flower buds, and judging whether the sweet cherry to be evaluated is high-yield type according to the proportion value. The method provided by the present application takes the proportion of each flower bud containing 2-4 flowers in all flower buds in the sweet cherry new germplasm of the same tree age grafted onto different rootstocks (arborization rootstock, dwarfing rootstock) as the evaluation index, and rapidly identifies the high-yield property of the sweet cherry new germplasm. The method simplifies the identification process and greatly shortens the identification period.

The present invention provides a method for quickly growing seedlings from sweet cherry seeds, which relates to the field of growing seedlings from sweet cherry seeds. The method includes separating the pulp from the fruit pit of the sweet cherry fruit, putting the fruit pit into a fine mesh bag, washing it, soaking it in a disinfectant solution, rinsing it, and drying the fruit pit after draining the water; putting the stored fruit pit into a container, soaking it, floating out the floating seeds, and reserving the fruit pit at the bottom for later use; knocking the reserved fruit pit to break the pit shell, taking out the kernel, and using the kernel as the sweet cherry seed; after rinsing the sweet cherry seed, putting it into an exogenous hormone solution, and standing it still in the dark at room temperature, disinfecting the treated sweet cherry seed, placing the disinfected sweet cherry seed on a culture dish lined with wet filter paper, and putting the culture dish into an artificial climate chamber; when 90% of the sweet cherry seeds turn green, sowing them in a nutrient bowl filled with vermiculite, watering, and spraying a nutrient solution on the emerged seedlings; hardening off the seedlings, and when the seedlings grow to 10 cm in height, transplanting them to a greenhouse. The present invention can effectively and quickly break the dormancy of the seeds and enable the sweet cherry seeds to germinate quickly.

The present invention provides a sweet cherry glycosyltransferase PavUGT48, its encoding gene, and applications. The amino acid sequence of the sweet cherry glycosyltransferase PavUGT48 is shown in SEQ ID No. 1, and the nucleotide sequence of the gene encoding the sweet cherry glycosyltransferase PavUGT48 is shown in SEQ ID No. 2. The sweet cherry glycosyltransferase PavUGT48 provided by the present invention can be used to catalyze cyanidin to produce cyanidin 3-O-glucoside and catalyze prunin to produce amygdalin, thereby improving the quality of sweet cherry fruit. It can be used to cultivate new high-quality sweet cherry varieties and industrially produce cyanidin 3-O-glucoside and amygdalin.

The invention belongs to the field of breeding, and particularly relates to an efficient breeding method for sweet cherries. According to the method, the cherry trees are protected through the isolation shed, the self-fruitful variety is selected, and efficient breeding of the sweet cherries is achieved.

The utility model discloses a retractable greenhouse for sweet cherry planting, and relates to the technical field of retractable greenhouses, the retractable greenhouse comprises a retractable bottom rod, the top of the retractable bottom rod is fixedly connected with a ground cone, the top of the retractable bottom rod is inserted with a bidirectional connecting pipe, the top of the bidirectional connecting pipe is inserted with a three-way connecting pipe, and the top of the three-way connecting pipe is connected with the ground cone. A bent ceiling is inserted into the top of the three-way connecting pipe, one side of the bent ceiling is in threaded connection with a fixing assembly, one side of the fixing assembly is fixedly connected with a motor box, the interior of the motor box is fixedly connected with a motor body, and the output end of the motor body is in spline connection with a transmission rod. Through the arrangement of the transmission assembly and the connecting rod, the effect of storing and covering a greenhouse film is achieved in the using process, the greenhouse film can be shrunk or covered through the transmission assembly, workers are prevented from manually covering and storing the greenhouse film, the labor intensity is relieved, manpower and time cost is saved, and the working efficiency is improved. And the working efficiency is greatly improved.

The present invention discloses the use of the PavKLUH gene and its transcription factor PavRAV2 in regulating the fruit size of sweet cherries. Through experiments such as overexpressing PavKLUH ectopically in Arabidopsis thaliana and transiently silencing the expression of the PavKLUH gene in sweet cherry fruits, it is found that overexpressing or overexpressing the PavKLUH gene in sweet cherries can make the cherry fruits larger. The present invention further uses yeast one-hybrid screening library to obtain the binding protein PavRAV2 upstream of the PavKLUH promoter, and then uses EMSA and dual luciferase experiments to confirm that PavRAV2 can directly bind to the PavKLUH promoter and inhibit the expression of the PavKLUH gene. Combining with the experiment of transiently silencing the PavRAV2 gene in sweet cherry fruits, it is determined that PavRAV2 negatively regulates the expression of the PavKLUH gene, and inhibiting or silencing the PavRAV2 gene in sweet cherries can make the sweet cherry fruits significantly larger.

The invention relates to the technical field of fruit tree planting, in particular to a semi-root-limited cultivation method and system for sweet cherries in coastal saline-alkali soil facilities. A salt discharging system of non-woven fabric, a gravel guiding and discharging layer, a perforated PVC drainage pipe, an underground water storage pond and a floating ball pump is built in the greenhouse in the row direction; a root control container with the height of 0.5-0.6 m and the diameter phi of 1.0-1.2 m is semi-buried on the gravel layer, surface soil, a culture medium, fresh water sand and organic fertilizer are filled according to the ratio of 4: 2: 3: 1, double-pipeline drip irrigation and four constant-pressure drippers are arranged, and a closed loop of'salt control input and salt discharge output 'is formed. By matching with AM bacteria and silicon-based foliar nutrition, salt reversion can be inhibited, cooling and ventilation can be realized, lateral capillary roots can be promoted, the fruit setting rate, the yield of a single plant and the fruit uniformity can be improved, and the method is suitable for being popularized in southern warm and wet coastal saline-alkali soil.