75 results about "Fruit development" patented technology

The invention relates to the technical field of farmland fertilization management, and discloses an intelligent fertilization management method and system based on machine learning. The method comprises the steps that a soil parameter set and an environment parameter set of a target farmland are collected, soil parameters comprise soil humidity, nitrogen phosphorus and potassium content and pH value, and environment parameters comprise illumination intensity, temperature and rainfall; constructing a soil nutrient dynamic change model according to historical data, and predicting a soil nutrient consumption trend in a future preset period; generating an initial fertilization scheme based on the nutrient consumption trend and the crop growth stage characteristics; monitoring the growth state of crops in real time by using a multi-mode sensor, and obtaining a leaf surface color index, a stem height and a fruit development progress to form a growth state data set; and inputting the growth state data set and the initial fertilization scheme into a fertilization decision model, and comparing growth state deviation to adjust the nutrient distribution ratio to generate an optimized fertilization scheme.

The invention relates to the technical field of navel orange tree cultivation and physiological regulation and control, and discloses an ethylene regulation and control-based low-temperature dry-water prevention and control method for late-maturing navel oranges before harvest. According to the method, the dry-water rate of the navel oranges in a complex environment is reduced through multi-dimensional accurate regulation and control, and the ethylene inhibitor spraying time is flexibly adjusted according to the climate of a planting field and the fruit development process; the ethylene synthesis can be intervened at the optimal opportunity; the method comprises the following steps: preparing a solution according to a formula, scientifically grouping navel orange trees with consistent growth vigor, accurately spraying inhibitors with two concentrations, ensuring that the agent uniformly acts on fruits and leaves, dynamically adjusting the spraying frequency according to rainfall conditions, avoiding agent loss, and after spraying, performing fertilization and watering in combination with a fruit tree phenological period fertilizer requirement rule and soil moisture content. A good growth environment is created for navel orange trees, the control effect is verified and the scheme is optimized through data detection and record analysis of the system after fruits are picked, and the low water rate of the navel oranges under different severe conditions can be reduced by combining the steps, so that the fruit quality and yield are guaranteed.

The invention discloses application of a ZaSPL7 gene in regulation and control of hairy bodies and parthenocarpy of plants, and belongs to the technical field of gene engineering. The gene ZaSPL7 for regulating and controlling organ development of the green prickleyash is separated from cDNA of the green prickleyash and is overexpressed in a plant, the number and density of epidermal hair of the transgenic plant are obviously increased, meanwhile, the number of fruit seeds is obviously reduced, even part of plants have seedless fruit phenotypes, and the unisexual setting rate is increased. The results show that the green prickleyash SPL7 gene may coordinate epidermal hair and fruit development by integrating development stage signals, and has important application value in regulation and control of epidermal appendages (such as thorns and hair) formation and apomixis, and the mechanism provides a new idea for simultaneous improvement of plant stress resistance (by reducing the thorns) and fruit commodity (by seedless). Important theoretical basis and technical support are provided for breeding of the green prickleyash, and the method has a wide application prospect.

The invention relates to the field of agricultural yield prediction, and particularly discloses a crop yield prediction method and system based on artificial intelligence, and the method comprises the steps: S1, recognizing crop types through the spectral features of a remote sensing image and field sampling data, and dividing corresponding key growth stages; s2, collecting multi-source feature data for each key growth stage, wherein the multi-source feature data comprises remote sensing image data, an extracted normalized difference vegetation index NDVI, an enhanced vegetation index EVI and a topographic factor; acquiring hourly temperature, humidity, precipitation and illumination intensity of each sub-region through a distributed sensor according to micro-meteorological data; obtaining plant height, leaf area index and fruit development parameters through unmanned aerial vehicle three-dimensional imaging according to crop phenotype data; by adopting the technical scheme of the invention, the terrain, microclimate and crop dynamic growth characteristics can be accurately fused, and the problem of non-uniform sample distribution is solved, so that the accuracy and regional adaptability of yield prediction are improved.

The invention relates to the technical field of agriculture, and particularly discloses a cherry tomato whole growth period fertilization method, which comprises the following steps: 10-15 days before field planting, deeply ploughing soil for 30-35 cm, exposing for 3-5 days, adjusting the pH value of the soil to 6.0-7.0, and adjusting the EC value to be less than 1.2 mS / cm; 600-800 kg / mu of carbon enzyme microbial fertilizer and 50-60 kg / mu of compound fertilizer are applied to the upper layer; 150-200 kg / mu of carbon energy soybean organic fertilizer and 100-120 kg / mu of silicon-calcium-magnesium fertilizer are applied to 0-20 cm of the middle layer; applying cattle and sheep manure of which the total amount is greater than or equal to 2000 kg / mu and fermented soybean meal of which the amount is 200-250 kg / mu to 20-40 cm of After fertilization, 300 times of EM bacteria liquid is immediately sprayed; according to the method, layered fertilization is combined with the microbial agent to activate soil activity, a long-acting nutrition library is constructed in a base fertilizer stage, and drip irrigation and foliage topdressing schemes at key nodes in a growth period are matched, so that the absorption efficiency of the cherry tomatoes on nitrogen, phosphorus, potassium and medium trace elements is remarkably improved; through fulvic acid, brassinolide and other substances, crop physiological status is rapidly adjusted, stress of high temperature, cloudy and rainy days and the like is effectively resisted, and fruit development continuity is guaranteed.

The invention discloses a high-yield cultivation method for muskmelons in sand land in South China, which belongs to the technical field of agricultural cultivation and comprises the steps of preparation before cultivation, seedling raising, transplanting, field management and harvesting. The variety is a heat-resistant and moisture-resistant type with good resistance, and is matched with a steel pipe type multi-span greenhouse and an insect-proof net to create a suitable growth environment. The planting crop rotation of three crops in one year makes full use of resources. Water and fertilizer integrated irrigation is adopted, and water and fertilizer are accurately supplied. Fine management is conducted in the seedling raising link, and robust melon seedlings are cultivated. Details are emphasized during transplanting, and the survival rate is guaranteed. Field management is carried out according to the growth rule of muskmelons, precise regulation and control are carried out in all stages such as vine stretching, vine binding and branch pruning, and diseases and pests are specifically prevented and controlled. And timely harvesting after 35-44 days of maturation. The cultivation method has the advantages that the cultivation method adapts to the special environment in South China, diseases and insect pests are reduced, water and fertilizer are accurately supplied, plant growth and fruit development are promoted, the land resource utilization rate is increased, the yield and quality of the thick-skin muskmelons can be effectively improved, and good benefits are brought to farmer households.

The invention provides a syzygium samarangense potted plant with fruits and a cultivation method and application thereof. The cultivation method comprises the step of carrying out half-cutting treatment on syzygium samarangense fruiting mother branches with flower buds, wherein the half-cutting treatment is combined operation of beveling and longitudinal cutting. According to the cultivation method provided by the invention, the technical problems that branches with flowers and fruits are difficult to root and easy to fall off are effectively solved through the synergistic effect of half-cut wound induction and rooting agent medium mediated slow-release root promotion and preferably in combination with microenvironment regulation and control of an interface type rooting matrix bag; and a complete technical scheme is provided for large-scale and efficient production of the syzygium samarangense bonsai with fruits. The potted syzygium samarangense with fruits cultivated by the method has robust and developed root systems and good fruit development, has both ornamental and picking functions, and has high ornamental value and economic value.

The application relates to a rice key growth period monitoring method based on time sequence SAR and accumulated temperature, wherein the method comprises the following steps: acquiring year time sequence VH curves and year time sequence VV curves corresponding to time sequence SAR of rice in a target area, performing filtering and smoothing processing on the year time sequence VH curves to obtain a smooth curve corresponding to the year time sequence VH curves, and determining a rice sowing period according to the smooth curve; extracting accumulated temperature information from the rice sowing period to a rice booting to heading date of the target area, and constructing multi-scale information of the year time sequence VV curves; extracting tillering period information and fruit development and maturation period information in the key growth period of the rice according to the multi-scale information, and extracting booting to heading period information in the key growth period of the rice in the target area based on the multi-scale information and the accumulated temperature information. Therefore, the problems that crop growth period information is not fully utilized and climate factors are not considered in the existing rice monitoring method are solved.

The invention discloses a cell wall associated kinase gene VvWAKL8 for regulating and controlling texture formation of grape fruits and application thereof, the cell wall associated kinase gene VvWAKL8 is cloned from the grape fruits for the first time, the nucleotide sequence of the cell wall associated kinase gene VvWAKL8 is as shown in SEQ ID NO.1, and the coded amino acid sequence is as shown in SEQ ID NO.2. Research finds that the VvWAKL8 is specifically and highly expressed in the hard-pulp grape variety, and subcellular localization analysis shows that the protein is localized in a cell membrane; instantaneous overexpression of VvWAKL8 in grape fruits can significantly improve fruit hardness and regulate pectin metabolism related gene expression, and verifies the core function of VvWAKL8 in fruit texture formation. The research reveals that the VvWAKL8 is a key gene for regulating and controlling the grape fruit texture for the first time, a brand new gene resource is provided for molecular marker-assisted breeding of a new storage-resistant grape variety, and an important foundation is laid for deeply analyzing a cell wall signal to regulate and control a molecular mechanism of fruit development.

The present application belongs to the technical field of plant cultivation, and particularly relates to a biological fertilizer for relieving fruit drop of Taiwan long fruit mulberry and application. The biological fertilizer comprises animal manure, soybean meal powder, humic acid, peat soil, coconut bran, bran, fermentation liquor of a compound microbial agent, seaweed extract powder, amino acid chelated iron and borax; wherein the compound microbial agent comprises Bacillus amyloliquefaciens and Pseudomonas aeruginosa. The above biological fertilizer is balanced in nutrients, is beneficial to enhancing the resistance of the mulberry to adversity and reducing fruit drop caused by stress; in addition, the Bacillus amyloliquefaciens and the Pseudomonas aeruginosa are used synergistically, and the two jointly build a healthy rhizosphere micro-ecology, thereby guaranteeing nutrient supply for fruit development from the root source. Therefore, the technical scheme of the present application can not only significantly reduce the fruit drop rate, but also improve the single fruit weight, soluble solid content and anthocyanin content of mulberry, and improve the flavor and color of the fruit.

This invention discloses a method for cultivating long-branch Buddha's Hand fruit by cutting branches. The method uses a special variety called "Sunshine" and cultivates high-quality long-branch Buddha's Hand fruit by controlling the density of fruiting branches, spraying a 1500-fold dilution of 3% naphthaleneacetic acid during the bud stage to induce finger-shaped splitting, spraying a gibberellin-chlorpyrifos-potassium dihydrogen phosphate compound solution during fruit development to synergistically thicken the fruit stalk, adjusting the branch direction in summer to keep the fingertips pointing upwards, and combining this with midday shading. This method results in a 26.7% increase in fruit stalk diameter, a 6.4% increase in the number of fruits with open fingers, a 100.2% increase in the number of long-branch fruits, a 20.0% reduction in the number of fruits with fruit surface burns, and an 8.8% reduction in the number of fruits that fall off after harvest. The number of fresh-cut long-branch marketable fruits increases by 100.2%, and the long-branch marketable fruit rate reaches over 70%. Overcoming the bottlenecks of traditional cultivation such as weak fruiting ability of long branches, thin and weak fruit stalks that are easy to fall off, and easy damage to the fruit surface, the cultivated long-branch Buddha's Hand fruit has the advantages of being resistant to fruit drop, having a smooth fruit surface, having a well-developed fruit shape, and having suitable branches. It can be directly used for cut flower scenarios such as vase decoration and bouquet matching, creating a new category of Buddha's Hand cut fruit and significantly enhancing its ornamental and economic value.

The invention provides a method for improving the VIGS research efficiency of solanaceous vegetables and application of the method, and belongs to the technical field of biology. Specifically, a UP gene interference fragment and a target gene interference fragment are connected in series, double-gene silencing is utilized, UP is used as a reporter gene, and whether the target gene is silenced or not is judged according to orientation changes of flowers and fruits of a plant before and after UP gene silencing, so that gene silencing judgment is more visual and efficient; the method can greatly improve the screening efficiency of functional genes, has important significance in accelerating screening and functional identification of solanaceous vegetable genes, and particularly has wide application prospects in exploration and functional identification of control genes related to flower and fruit development, fruit ripening and metabolism.

The invention relates to the technical field of plant cultivation and nutrition, and discloses a treatment method for enhancing coloring and sugar increasing of citrus fruits based on 5-aminolevulinic acid. And phosphite serving as a carbon immobilization catalyst and polyhydric alcohol serving as a product transfer accelerant are creatively compounded. The preparation method comprises the key step of accurately regulating and controlling the pH value of the system so as to ensure the stability and efficient absorption of the preparation. According to the application method, foliage spraying is carried out in specific time windows of the young fruit stage and the expansion stage of citrus, so that the effect of the preparation is matched with the key physiological rhythm of fruit development. Through the synergistic effect of the composition and the method, the photosynthetic whole chain of light harvesting-carbon sequestration-transportation is systematically optimized, the soluble solid content and the sucrose content of the citrus fruits can be remarkably increased, the color turning of the peel is effectively promoted, and the method is safe, stable and outstanding in effect.

The present application relates to the field of molecular biology, in particular to a kind of molecular marker related to the ascorbic acid content of tomato fruit development and its application, the present application is by screening the expression of ascorbic acid related gene-eGWAS data, identify a significant correlation with the ascorbic acid of tomato fruit structural variant (SV). The SV is located at the downstream 397bp of the rate-limiting enzyme GDP-L-galactose phosphorylase gene (GGP2, Solyc06g073320) of ascorbic acid synthesis pathway, it is a 25bp insertion / deletion at 42845487 of tomato chromosome 6. The present application further verifies the existence of the SV in resequencing tomato germplasm and develops molecular marker, and the results show that the molecular marker can be applied to the parent selection of high ascorbic acid tomato fruit selection.

The application will AtGRF4 The gene is stably integrated into the cucumber genome, realizes generation inheritance, and discovers that the overexpression AtGRF4 The gene can directly activate the key path of fruit development, significantly promote fruit cell division and expansion, and directly realize fruit growth. Compared with the traditional regulation mode, the present application has important practical significance and application value for developing high-efficiency and stable cucumber fruit growth technology. The present application does not need complex equipment, is simple to operate, is suitable for existing facility cultivation system, and simultaneously AtGRF4 The function is conservative, and the stable genetic transformation technology can be expanded to other melon and fruit crops, expand the application range, give consideration to high yield and operation convenience, and meet the needs of large-scale production.

This invention discloses a method and system for predicting goji berry yield based on deep learning. The method constructs a time-series sample set consisting of synchronously acquired images, environmental data, timestamps, and spatial locations; it uses a model that fuses image and environmental features to identify fruit bounding boxes, developmental stages, and confidence levels; it constructs fruit development trajectories through time-series tracking and divides crop windows based on the first flowering time; it introduces stage weights and confidence levels for joint correction in crop yield calculation; it dynamically corrects for time overlap between crop windows by calculating the overlap ratio, and finally accumulates and fuses the results to obtain the predicted total yield. This invention achieves high-precision prediction of goji berry crop yield and total yield, solving the problems of confusion, duplication, or omission in statistics in multi-crop scenarios, and providing a reliable basis for precise goji berry harvesting and agronomic management.

The present invention discloses a transcription factor for regulating tomato growth, a research method, and a method for preparing a tomato mutant plant. The gene accession number of the transcription factor for regulating tomato growth is Solyc01g106030, its protein sequence is shown in SEQ ID NO.1, and its encoding DNA sequence is shown in SEQ ID NO.2. The transcription factor has a regulatory effect on tomato growth, specifically for regulating tomato fruit development, plant height, and / or the number of lateral branches. Therefore, by changing the expression specificity of the transcription factor or mutating the transcription factor, the downstream gene regulated by the transcription factor is changed, thereby regulating tomato fruit development, plant height, and / or the number of lateral branches.

This invention belongs to the field of plant genetic engineering technology, specifically relating to the application of the PoPILS1a gene in regulating plant seed development. The coding sequence of the PoPILS1a gene is shown in SEQ ID NO:1. This invention is the first to discover that PoPILS1a is specifically expressed in peony seed tissues, and the expression level varies significantly at different developmental stages. The PoPILS1a protein is located in the endoplasmic reticulum. In Arabidopsis thaliana positive lines with overexpression of PILS1a, the attachment point between pods is significantly shortened, and the number of pods distributed within a limited inflorescence length is greater. Simultaneously, the length and weight of pods at corresponding positions show significant differences compared to the control, clarifying that PILS1a can promote fruit development and has good practical application value.

The invention discloses application of a VcBR6OX1 gene in seedless fruit formation, and belongs to the technical field of molecular biology. According to the invention, the BR6OX1 gene in blueberry is transferred into tomato through a genetic transformation technology, and phenotype identification and physiological and biochemical index determination on transgenic tomato prove that overexpression of VcBR6OX1 can promote formation of seedless fruits. Through genetic transformation and functional analysis, the action mechanism of BR6OX1 in fruit development is disclosed. Meanwhile, transcriptome analysis is carried out on transgenic tomatoes and wild tomatoes, downstream genes responding to BR6OX1 are found, a theoretical basis is provided for development of seedless fruits, and a foundation is laid for application of the genes in seedless fruit breeding.

The application relates to the technical field of farmland fertilization management, and discloses an intelligent fertilization management method and system based on machine learning. The method collects a soil parameter set and an environmental parameter set of a target farmland, the soil parameters include soil humidity, nitrogen, phosphorus and potassium contents and pH values, and the environmental parameters include illumination intensity, temperature and rainfall; a soil nutrient dynamic change model is constructed according to historical data, and soil nutrient consumption trends in a future preset period are predicted; an initial fertilization scheme is generated based on the nutrient consumption trends and crop growth stage characteristics; a multi-modal sensor is used to monitor a crop growth state in real time, growth state data sets are obtained by acquiring leaf color indexes, stem heights and fruit development progress, and the growth state data sets and the initial fertilization scheme are input into a fertilization decision model; and through comparison of growth state deviations, nutrient proportions are adjusted to generate an optimized fertilization scheme.

The invention discloses a method for cultivating heterosis litchi resources by using male sterile litchis, relates to the technical field of fruit tree cultivation and breeding, and aims to solve the problems of unstable flowering phase overlapping degree of domestic litchi varieties and 'Kom', low pollination efficiency, low fruit retention rate and the like. The invention provides a method for cultivating heterosis litchi resources by using male sterile litchis, which comprises the following steps: selecting 'Kom' as a female parent, respectively grafting the 'Kom' and an extremely early male parent 'Sanyuhong' to litchi seedling stocks, unifying the growth environment and synchronizing the flowering phase. Secondly, compound fertilizer is applied and leaflets are removed when the spica is extracted, so that the quality of the spica is optimized, and the pollination efficiency is improved. Then, closed girdling is conducted on the bases of the fruit bearing branches to protect fruits, nutrients are regulated, fruit dropping is reduced, and it is ensured that the fruits grow to be mature. And finally, collecting mature fruits, sowing to obtain F1-generation plants, performing heterosis evaluation, and screening early-maturing and high-quality new varieties.

This invention discloses a method for recommending germplasm resources and hybrid combinations for new peach variety breeding, belonging to the field of agricultural bio-breeding technology. The method involves collecting phenotypic observation data and genotypic detection data of peach germplasm resources; determining phenotypic characteristics including fruit development duration and dynamic parameters of sugar and acid accumulation, and generating a phenotypic dataset through genetic association analysis; generating a preliminary genetic similarity matrix through genetic distance calculation and kinship analysis, and obtaining a standardized genetic similarity matrix by correcting phenotypic expression stability data; inputting the dataset into a genetic breeding digital model, adjusting the germplasm unit arrangement and genetic effect parameters to update the model; acquiring cultivation environment data, determining phenotypic segregation patterns under different breeding cycles, analyzing the correlation between phenotypic segregation patterns and cultivation environment data, and generating hybrid combination recommendation results. This invention can provide systematic guidance for parent selection in peach breeding work, improving breeding efficiency.

This invention discloses VvENO2 The application of genes in regulating plant fruit development belongs to the field of plant genetic engineering technology. Previous research in this invention found that hydrogen peroxide (H2O2) treatment significantly promotes grape fruit ripening. Transcriptome analysis of H2O2-treated grapes revealed… VvENO2 Gene expression levels decreased. To further clarify... VvENO2 The role of the gene in plant fruit development was investigated through subcellular localization experiments and promoter activity analysis, demonstrating its nucleus location and that its promoter can activate the GUS reporter protein. Furthermore, treatment with ABA and H2O2 enhanced its activity. In addition, transgenic Arabidopsis and transgenic grape were constructed, and phenotypic observations confirmed... VvENO2 Genes negatively regulate the ripening of plant fruits. This invention provides a new regulatory site for breeding early-maturing plant varieties.

The invention discloses a method for promoting effective coloring of Nouronia queen grapes. According to the method, a five-dimensional superposition regulation and control system with girdling induction, shading and bag removal, water and nutrient control, exogenous hormone supplement, spectral energy enhancement and canopy microclimate optimization is constructed, grape fruit development and pigment metabolism processes are accurately intervened in stages, and anthocyanin synthesis and accumulation are systematically promoted. According to the method, the fruit coloring rate and the coloring uniformity are improved, the effective coloring window period is prolonged, and the commodity fruit rate and the fruit sugar-acid ratio are remarkably increased. Field tests prove that the fruit coloring rate can be increased to 90% or above, the commodity fruit rate can be increased to 80% or above, and the output value per mu is remarkably increased. The invention provides a systematic solution for solving the technical problems of poor coloring, unstable quality and the like of the Naironia queen grapes, and has good popularization and application values.

The invention discloses an FvePREs transcription factor and application thereof in promoting strawberry fruit expansion, compared with a traditional expansion agent, the scheme has the advantages that the safety is improved, and the existing expansion agent may cause malformed fruits and hollow fruits and has chemical residue risk. The FvePREs transcription factor regulates and controls fruit development through a molecular level, exogenous hormones are not needed, and chemical pollution and potential food safety hazards are avoided; the scheme is more accurate in action mechanism, the existing biological agent (such as a formula containing alginic acid and brassinolide) needs to depend on external application, and the effect is influenced by environmental factors. The gene technology directly acts on a plant endogenous regulation and control system, and the synergistic effect of fruit expansion and quality optimization is achieved by activating or inhibiting specific gene expression; the scheme has sustainability, the overexpression FvePREs transcription factor can change the genetic characteristics of strawberries, so that the strawberries can keep a good expansion effect in the long-term planting process, dependence on external chemical substances is reduced, and sustainable agricultural development is facilitated.

The invention discloses bradyrhizobium yuanmingense ASHBWH-23-9 with functions of promoting growth and inhibiting aspergillus flavus and application of the bradyrhizobium yuanmingense ASHBWH-23-9, and the preservation number of the bradyrhizobium yuanmingense ASHBWH-23-9 is CCTCC (China Center for Type Culture Collection) M 20253053. The innovation of the invention lies in that the dual functions of generating cyclopentanone antibacterial volatile matters and efficiently symbiotically fixing nitrogen are integrated in the bradyrhizobium in the circular and clear garden for the first time. The molecular mechanism is that the generated volatile substance can accurately down-regulate the expression of a toxin synthesis key gene and a spore development core gene in aspergillus flavus. Compared with an existing microbial agent with a single function, nodulation and nitrogen fixation promotion and aspergillus flavus inhibition at the source can be synchronously realized at the rhizosphere of the peanuts through one-time inoculation, and particularly, aiming at the characteristics of overground flowering and underground fruiting of the peanuts, the microbial agent continuously plays a role in bacteriostasis and antipoison in soil in the pod development period.

The invention relates to an apple quality improving type synergistic water-soluble fertilizer and a production method thereof.The apple quality improving type synergistic water-soluble fertilizer is prepared from 30-40 parts of calcium nitrate tetrahydrate, 15-25 parts of magnesium nitrate hexahydrate, 30-40 parts of beet molasses, 0.4-1 part of sodium octaborate, 0.4-1 part of zinc nitrate hexahydrate, 0.1-0.3 part of vitamin B, 1-2 parts of sodium glutamate and 2-3 parts of yeast extract. According to the present invention, the formula has characteristics of scientific and reasonable ratio, comprehensive and balanced nutrition, reasonable supply of calcium, boron and other elements, apple fruit appearance improving, physiological disease reducing, and commodity value increasing; various nutrient elements are cooperated with bioactive substances, so that accumulation of nutritional ingredients of the fruits can be promoted, the sugar-acid ratio is increased, and the taste is improved; the synergistic effect of various raw materials can enhance stress resistance of apple trees, reduce stress loss and guarantee normal growth and fruit development.

The invention discloses application of a hippophae rhamnoides Hgfw3.2 gene in regulation and control of fruit size, and belongs to the technical field of biological genetic engineering. The nucleotide sequence of the Hgfw3.2 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the protein coded by the Hgfw3.2 gene is as shown in SEQ ID NO. 2. According to the invention, the key gene Hgfw3.2 for regulating and controlling the fruit size is cloned and identified from sea-buckthorn in Jiangcumin for the first time, and the positive regulation and control function of the gene in fruit development is defined. Through overexpression of the Hgfw3.2 gene in a tomato system, the weight, the longitudinal diameter and the transverse diameter of a fruit are obviously increased, the volume of the fruit is obviously increased, and the promotion effect of the gene on the size of the fruit is proved. Compared with the prior art, the invention provides a new gene resource from an extreme environment adaptive plant sea-buckthorn, enriches a gene pool for regulating and controlling the fruit size, and provides a new molecular target for improving the fruit size.

The application discloses a kind of micro-fertilizer application methods suitable for oat grass green production in Huanghuai region, and relates to the technical field of livestock planting, first select good land, then oat grass seed is soaked with micro-fertilizer and seed dressing fungicide, both provide nutrition for seed growth, and can eliminate viruses on the surface of seed, fundamentally eliminate disease hazards, then spray manganese sulfate, ferrous sulfate, zinc sulfate mixed micro-fertilizer on leaf surface when height is 15 cm, reach the combination of nutrition and health care, guarantee stem green and disease-free spot.In flowering period, spray ammonium molybdate and boric acid mixed micro-fertilizer on leaf surface, prevent disease on fruit, conducive to fruit development, ensure the health of fruit.The application method is simple and easy to operate, efficient and environmentally friendly, and has strong operability, easy to popularize in production, and conducive to oat grass green and high-quality production.

The invention discloses an application of a tomato SlMNSI1 gene in regulation and control of fruit quality. The nucleotide sequence of the gene is shown as SEQ: NO.1. The invention also discloses an application of the tomato SlMNSI1 gene in regulation and control of fruit quality. The SlMNSI1 gene knockout stable genetic material is obtained through a CRISPR / Cas9 gene editing technology, and the influence of the SlMNSI1 gene knockout stable genetic material on the tomato fruit quality is researched. Results show that the deletion of the SlMNSI1 gene can lead to advanced maturation of tomato fruits, significant increase of ethylene release rate and soluble solid content, reduction of fruit size, but significant increase of contents of fructose, glucose, sucrose and various aldehydes and alcohols, thereby significantly improving fruit flavor. The SlMNSI1 gene plays an important role in regulation and control of fruit development and flavor substance accumulation, and a new gene resource and an application approach are provided for creating a new variety of early-maturing, small-fruit-type and high-flavor tomatoes.