The invention discloses a
yeast beta-
glucan preparation method based on
high pressure-microjet homogenization treatment, and belongs to the technical field of
zymosan extraction. The method comprises the following steps: carrying out
high pressure-microjet homogenization pretreatment on active dry
yeast, then carrying out autolysis treatment, then extracting beta-
glucan by an acid-alkali method, and finally carrying out
freeze drying to obtain a high-purity product. The core of the method is that the
yeast cell walls are subjected to physical wall breaking by adopting a
high pressure-microjet homogenization technology, and the extraction rate and the purity of the beta-
glucan are remarkably improved by combining with an optimized acid-alkali treatment process. Compared with a traditional enzymolysis method, the method has the advantages that damage of exogenous
enzyme to the beta-glucan structure is avoided, the purity of the obtained product reaches 84% or above, the content of
protein impurities is lower than 0.2%, a new way is provided for efficient utilization of yeast
cell wall resources, and the method is suitable for the fields of food,
medicine and health care products.