574 results about "Cell wall" patented technology

The invention relates to a curcumin-loaded MMP response type melittin nano-pellicle vesicle as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations. The preparation method comprises the following steps: firstly, preparing curcumin entrapped lipidosome; then carrying out culture amplification on engineering bacteria carrying melittin recombinant plasmids, extracting cell membranes after removing cell walls, and carrying out ultrasonic treatment and membrane extrusion to obtain melittin cell membrane nano-vesicles; fusing the curcumin lipidosome and the cell membrane nano-vesicles in an ultrasonic extrusion mode to obtain fused vesicles; and finally, carrying out surface calcium phosphate mineralization treatment on the fused vesicles to obtain the curcumin-loaded MMP response type melittin nano pellicle vesicles. The nano mycofilm vesicle prepared by the invention can be used for preparing antitumor drugs, and the biocompatibility and in-vivo stability of nanoparticles are improved by introducing a calcium phosphate coating; through combined delivery of melittin and curcumin, the anti-tumor effect is enhanced, so that the growth and proliferation of tumor cells are inhibited.

The present disclosure relates to compositions and methods comprising a yeast cell wall extract and a clay material admixed to a proteinaceous and / or cell wall extract(s) from bacterial or fungal fermentation from amino acid production. In another embodiment, the present compositions comprise a yeast cell wall extract, a clay material, and an algal material admixed to a proteinaceous and / or cell wall extract(s) from bacterial or fungal fermentation from amino acid production. This disclosure also relates to methods of utilizing the same to sequester and / or adsorb mycotoxins internal or external to an animal, thereby reducing and / or preventing the toxic and harmful effects of mycotoxin contamination and / or infection in animals. Specifically, application of the present methods and compositions are found in the dietary administration and / or co-administration of the present compositions to animals in animal feed and / or animal production systems, as well as in therapeutic, prophylactic, and research applications.

The invention discloses a co-fermentation method based on sphingomonas and a rhodiola rosea extracting solution and application of a product, and belongs to the technical field of microorganisms. By utilizing a special rich enzyme system of sphingomonas and combining with an early-stage composite enzymolysis process and synergistic effect, the wall breaking efficiency of rhodiola rosea cell walls and the conversion degree of active components of rhodiola rosea are greatly improved, the bottlenecks of low efficiency and low bioavailability of a traditional rhodiola rosea extraction method are successfully overcome, and the rhodiola rosea extraction method is suitable for industrial production. The functional components such as salidroside and flavone can be fully released and converted. Secondly, different from non-symbiotic bacteria (such as lactic acid bacteria and saccharomycetes) which are commonly used for fermentation in the prior art, a potential symbiotic bacterium of sphingomonas XJL-TP07 from a special environment is innovatively selected and has better metabolic suitability with the rhodiola rosea component, the fermentation process is milder and safer (no irritant is produced), and the rhodiola rosea fermentation method is suitable for large-scale production. And a synergistic interaction effect can be generated through a unique biotransformation path.

The invention relates to the technical field of deep processing of vegetable fat, in particular to a preparation method and application of squalene-containing macadamia nut oil. Through the synergistic effect of directional enzymolysis conversion and low-temperature step-by-step extraction and the combined effect of the three enzymes (cellulase destroys cell walls, lipase releases grease and beta-glucosidase removes inhibitors, and the three enzymes form a wall breaking-oil releasing-synergistic synergistic reaction) by utilizing a compound enzyme which is fixedly matched and proportioned. The compound enzyme can be used for synchronously treating cellulose, lipid and glycoside substances, so that the release efficiency of squalene is improved; and step-by-step extraction is utilized to reduce extraction impurities, so that the content of squalene in the macadamia nut oil can be increased, and the extraction and enrichment of squalene in the macadamia nut meat can be realized.

The invention provides a vibration noise detection device and method of a wall breaking machine, and mainly relates to the field of vibration noise detection. A vibration noise detection device of a wall breaking machine comprises a positioning mechanism arranged in a detection chamber and a plurality of noise detection mechanisms, coaxial placement and height adjustment of the wall breaking machine are achieved through clamping and positioning of the positioning mechanism, and the wall breaking machine can be located at the detection height on different workbenches. At least four noise detection mechanisms are arranged, each noise detection mechanism comprises a supporting arm, a supporting cavity is formed in the inner side of the front end of each supporting arm, a telescopic arm is arranged on each supporting cavity in a sliding mode, and a sound level meter and a distance sensor are arranged at the front end of each telescopic arm. The invention has the following beneficial effects: the invention creatively provides a structure and a method for respectively detecting noise by a plurality of workbenches, so that the noise intensity of the wall breaking machine in the daily use process can be more reasonably represented.

The invention belongs to the field of biosensing and microbiological detection, and relates to a staphylococcus aureus rapid counting method based on fluorescence confinement discretization. According to the method, oat bran is used as a natural carbon source, lysozyme active fragments are used as targeting ligands, targeting carbon quantum dots are synthesized through a one-step hydrothermal method, the targeting carbon quantum dots are modified by fluorescent dye SRB to obtain luminescence enhanced carbon quantum dots, and the luminescence enhanced carbon quantum dots can be specifically combined with cell walls of staphylococcus aureus, so that the staphylococcus aureus has stable fluorescence signals. And then, by taking the marked thalli as a core layer and a high-molecular polymer as a shell layer, preparing a core-shell nanofiber membrane by adopting a coaxial electrostatic spinning technology to realize spatial confinement and monomer discretization of the thalli, so that a group fluorescence signal is converted into a single-thalli distinguishable signal, and rapid and sensitive quantitative detection of the staphylococcus aureus is realized. The method is simple, convenient, high in sensitivity, suitable for rapid visualization and quantitative detection of staphylococcus aureus in foods such as dairy products, meat products and beverages, and wide in application prospect.

The invention relates to an extraction process of total flavonoids of gold-edge roses, and aims to solve the technical problems of low extraction rate, easiness in pyrolysis inactivation of components and poor stability in the existing extraction technology. The invention provides an enzymatic wall breaking-in-situ inclusion synergistic extraction scheme, which is technically characterized in that in a pure water system, the directional degradation effect of pectinase on a cell wall pectin matrix is utilized, and in-situ encapsulation protection of beta-cyclodextrin on dissolved active molecules is matched; enzymolysis and clathration processes are synchronously executed under a mild temperature condition, so that instantaneous steady-state locking is carried out on heat-sensitive flavone while physical wall breaking is realized. According to the process, the yield of total flavonoids is remarkably increased, oxidative degradation in the extraction process is effectively inhibited, and the obtained product is good in water solubility and physical and chemical stability. No organic solvent is needed in the whole process, conditions are mild, and a new path is provided for efficient extraction of active ingredients of the gold-edge roses.

The utility model discloses microalgae cell wall breaking equipment, and relates to the technical field of microalgae cell wall breaking equipment. The device comprises a treatment tank, a vertical rotating shaft is rotationally arranged at the middle end of the treatment tank, a plurality of stirring pieces distributed at equal intervals are fixedly arranged at the end, located in the treatment tank, of the rotating shaft, two symmetrically-arranged arc-shaped plates are arranged in the treatment tank, and a plurality of leakage grooves distributed at equal intervals are formed in the arc-shaped plates. Two long rods which are symmetrically arranged are fixedly arranged at the middle end of the arc-shaped plate; through linkage of the transfer plate, the rotating block, the swing plate, the long plate and other parts, the arc-shaped plate can push mixed raw materials at the inner edge of the treatment tank to be in continuous contact with the stirring piece, the over-static state of the conventional stirring mode is broken through, the raw materials are mixed more dynamically, the problem of long mixing time caused by insufficient mixing is avoided, and the mixing efficiency is improved. Therefore, the mixing efficiency is improved, and the problem that the treatment efficiency is affected by the mixing mode in the existing chemical wall breaking process is solved.

This invention discloses a honeycomb-structured, micro-pressure-enhanced, and efficacious plant-based soft film powder substrate and its preparation method, belonging to the field of cosmetic technology. The soft film powder substrate, by weight, comprises 15-30 parts sodium alginate, 5-12 parts calcium sulfate, 3-8 parts Tremella fuciformis polysaccharide, 20-40 parts fine-particle-size rice starch, 10-25 parts coarse-particle-size rice flour, 2-6 parts frozen-cell-broken Ophiopogon japonicus extract, and 2-6 parts frozen-cell-broken Polygonatum odoratum extract. This invention constructs a honeycomb porous framework using gradient particle size powders, optimizes the alginate-calcium ion intelligent cross-linking system, and prepares highly active plant extracts using ultra-low temperature freezing and cell wall disruption. It controllably constructs a honeycomb gel network, achieving excellent micro-pressure-enhanced penetration and moisturizing effects, while also possessing excellent thermal stability, cold storage stability, and light stability. This solves the technical problems of existing soft film powders, such as poor film-forming structure, weak penetration-enhancing effect, insufficient stability, and low utilization rate of active ingredients. The formula is mild and safe.

This application belongs to the fields of microbiology and cell biology, and provides a method for directly preparing germination-promoting fungal protoplasts from orchid protocorms, comprising: (1) physical homogenization; (2) filtration; (3) removal of plant contents and residual cell walls; (4) purification of mycelial clusters; and (5) release and purification of fungal protoplasts. Through the rational design of homogenization and purification steps, this application achieves high-yield, high-purity, and high-vitality preparation of germination-promoting fungal protoplasts, providing excellent materials for orchid gene variety breeding and genetic engineering operations.

The invention provides array hole porous graphite as well as a preparation method and application thereof, and relates to the technical field of porous graphite materials. The preparation method of the array hole porous graphite comprises the following steps: by taking biomass with an array hole structure as a raw material, removing lignin in the raw material by using a chemical reagent, and further strengthening an orientation structure of a cell wall by using a hot-pressing method, so as to obtain an orientation enhanced precursor; and further carbonizing to obtain the porous carbon skeleton. The preparation method comprises the following steps: preparing a carbon skeleton, introducing an element with a catalytic graphitization function into the carbon skeleton through impregnation, carrying out graphitization treatment under a high temperature condition to promote the graphitization degree of cellulose in a thermal conversion process, and promoting the removal of catalytic components at a high temperature by utilizing developed array holes of porous carbon, so that the catalytic performance of cellulose is improved. The array hole porous graphite with high thermal conductivity and high crystallinity is prepared. The problems that existing biological matrix array hole porous graphite is poor in crystallinity and low in heat conductivity are solved.

The invention relates to the technical field of traditional Chinese medicine extraction and concentration, and discloses an efficient and energy-saving traditional Chinese medicine micro powder extraction and concentration system. Firstly, plant cell walls are fully destroyed through a traditional Chinese medicine micro-powder wall breaking technology, and complete and quantitative conveying of micro-powder materials is ensured in combination with an air pressure balance residue removing system which is arranged in a storage tank and composed of an air disc, an air blowing pipe and an air blowing plug. And secondly, direct contact type hot air heating and stirring are adopted in the extraction tank, and a two-stage dust suppression structure of spraying and wet dust removal is arranged, so that efficient extraction and dust control are realized. And finally, the system recycles the residual components of the decoction dregs through a secondary extraction process, and adopts a membrane filtration and membrane concentration technology to replace the traditional heating concentration, so that the filtration and concentration of the decoction are completed at normal temperature. The method effectively solves the problems of low extraction rate, high energy consumption, easy damage of thermosensitive components and dust pollution in the traditional process, and realizes high efficiency, energy conservation and high quality of production of the traditional Chinese medicine extraction preparation.

The invention provides a food-grade method for extracting an edible nanocrystallization thin-wall cell wall from bamboo wood and application of the food-grade method, and the food-grade method comprises the following steps: carrying out thermal stripping treatment on a bamboo wood raw material through food-grade absolute ethyl alcohol, and then carrying out oxidation bleaching treatment by using a mixture aqueous solution of food-grade hydrogen peroxide and glacial acetic acid, and finally performing nanocrystallization refining, thereby obtaining the required edible nanocrystallized thin-walled cell wall, and the edible nanocrystallized thin-walled cell wall can be used as a dysphagia adjuvant or a fluid food thickening agent. The edible nanocrystallization thin-wall cell wall provided by the invention can be used for reducing the glycemic index (GI) of fluid food, increasing the viscosity and enhancing the stability of the fluid food, so that the fluid food can stably have a relatively high viscosity characteristic under different pH and temperature conditions, and the texture and taste of the fluid food can be effectively improved.

This invention relates to the field of oil extraction technology, specifically a method for subcritical low-temperature extraction of leek seed oil, comprising the following steps: (1) raw material pretreatment; (2) compound enzymatic hydrolysis cell wall breaking treatment; (3) subcritical low-temperature extraction; (4) integrated gradient low-temperature separation and solvent recovery purification. This invention, through the synergistic combination of raw material low-temperature pretreatment, compound enzymatic hydrolysis directional cell wall breaking, subcritical dynamic countercurrent extraction, and gradient low-temperature separation purification, optimizes the entire process from raw material structure modification, enhanced extraction mass transfer, to efficient product separation, systematically improving the efficiency and quality of leek seed oil preparation. In the raw material stage, hot air low-temperature drying and liquid nitrogen-assisted pulverization are used, which can reduce the moisture content of the raw material and refine the particle size without damaging the intracellular contents, reducing oil oxidation in subsequent processing, while simultaneously improving the enzymatic hydrolysis of the cell wall, laying the foundation for efficient extraction.

The application discloses a pretreatment device and method for kitchen organic slurry, and belongs to the technical field of organic garbage treatment. The pretreatment device comprises a homogenate reactor, a high-pressure pyrolysis reactor and a flash evaporation reactor which are sequentially connected. Different temperature treatments are arranged in the homogenate reactor, the high-pressure pyrolysis reactor and the flash evaporation reactor through the pretreatment device, so that the cell walls of organic matter in the kitchen organic slurry are broken, the biodegradability of the slurry is improved, and subsequent anaerobic fermentation treatment is facilitated.

This invention discloses methanogenic lyases with the same protein family annotation as PeiR lyases and their applications. These enzymes are obtained through homology screening based on the known characteristics of PeiR lyases targeting peptide bonds in the methanogenic cell wall, using the Pfam protein family database annotation. PeiR lyase proteins participate in the biological process of hydrolyzing archaea cell walls, effectively killing methanogens and reducing methane production. Proteins homologous to this protein have potential methane-reducing potential. This invention integrates rumen microbial metagenomic sequencing data, uses a series of bioinformatics software to screen methanogenic viral proteins, and combines Pfam functional domain annotation for homology analysis, ultimately identifying a series of lyases. These lyases were successfully expressed in a prokaryotic expression system, and in vitro gas production experiments also showed that the crude enzyme solution significantly reduced methane production.

The application belongs to the technical field of natural substance separation and extraction, and particularly relates to a kind of Haematococcus pluvialis multi-product cascade separation method based on eutectic solvent sequential extraction and enzyme membrane reactor coupling, comprising the following steps: (1) cell wall weakening pretreatment step; (2) water-soluble component extraction step; (3) pigment component extraction step; (4) astaxanthin extraction step; (5) enzyme conversion step. Through the synergistic effect of eutectic solvent sequential extraction and enzyme membrane reactor, efficient, green and continuous cascade separation of water-soluble components, pigments, astaxanthin and xylo-oligosaccharides in Haematococcus pluvialis is realized, and product purity and resource utilization are improved.

The application discloses a method for producing yellow fermentation acid slow-release organic fertilizer by using tylosin bacterial residue, and the method comprises the following steps: crushing the tylosin bacterial residue into powder by using a cell wall breaking machine; adding gamma-manganese dioxide and lignite in sequence, stirring and mixing uniformly, and fully contacting and reacting; irradiating and treating the obtained mixed system by using a high-energy electron beam to obtain yellow fermentation acid organic fertilizer; and compounding the obtained product with diatomite, and granulating after cooling to obtain the yellow fermentation acid slow-release organic fertilizer. The harmless treatment and resource utilization of the tylosin bacterial residue are realized, the product meets the actual production and application requirements, the product has good fertilizer efficiency, the residual antibiotics are removed synchronously, and the product has the dual functions of fertilizer efficiency and antibiotic removal.

In alternative embodiments, the invention provides vaccines, pharmaceutical compounds and formulations for diagnosing, preventing, treating or ameliorating Group A Streptococcus (GAS), Group C Streptococcus (GCS), or Group A Streptococcus (GGS), infections, or other pathogenic Streptococcus infections. In alternative embodiments, the invention provides compositions such as diagnostic tests, assays, immunoassays and test strips, and methods, for detecting or diagnosing the presence of a Streptococcal infection, e.g., Group A Streptococcus (GAS), Group C Streptococcus (GCS), or Group A Streptococcus (GGS), infections, or other pathogenic Streptococcus infections.

The efficacy and indication of donanemab do not depend on the Fc region and are subject to transit across cell walls. They can be expanded by using their scFvs conjugated with N-methyl lobe of transferrin protein connected with an environment-sensitive cleavable linker to prevent exocytosis of the scFv yielding high exposure inside body cells such as in the brain, eye, and cancer cells that overexpress transferrin receptors.

1. Name of the product in this design: High-speed blender. 2. Purpose of this design: It is used for cell wall breaking, food processing, and cooking of ingredients such as crushing, breaking, heating, and boiling. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model. 5. In the 3D diagram, the part labeled A is transparent.

The application discloses a sludge directional hydroxyl radical wall-breaking drying and deodorization method and system, and belongs to the technical field of sludge harmless and resourceful treatment. The application generates hydroxyl radicals in-situ in a sludge system by nano-catalytic coupling with a weak electric field, and makes the hydroxyl radicals preferentially act on microbial cell walls and malodorous substances by precisely controlling the concentration of the hydroxyl radicals, the reaction time and the pH of the system, so that cell wall breaking, water releasing, dehydration drying and source deodorization are realized. Meanwhile, the deep oxidation and mineralization of macromolecular organic matter are inhibited, and the organic carbon source and nutrient components are maximally reserved. The moisture content of the treated sludge is significantly reduced, the sludge is odorless, the organic matter retention rate is greater than or equal to 85%, and the sludge can be directly connected with anaerobic digestion, aerobic fermentation composting or land improvement utilization. The application has the characteristics of fast treatment speed, low energy consumption, no secondary pollution and strong resource connection, solves the problems of long cycle of traditional processes, malodorous emission and organic matter loss caused by excessive oxidation, and is suitable for efficient pretreatment of high-organic sludge such as municipal sludge and food sludge.

The present invention relates to the use of a therapeutic agent based on cell wall fragments of a virulent strain of Mycobacterium tuberculosis-complex for the preparation of a drug for the treatment of patients with active tuberculosis.

The invention provides a conversion method of rare ginsenoside Rg5. According to the method, through a vacuum permeation technology, gas in cell gaps of ginseng roots is removed in a vacuum environment, a permeation barrier of cell walls is weakened, a micro-channel is formed, and then a mixed solution of methanol and n-butyl alcohol is injected for primary extraction; after the normal pressure is recovered, the mixed solution can be driven by differential pressure to quickly fill a micro-channel and be better dissolved with the ginseng roots, a more efficient extraction environment is created, extraction is carried out under the low-temperature condition of 30-50 DEG C, thermal degradation of PPD can be effectively avoided, meanwhile, dissolution of impurities such as polysaccharide and protein is reduced, and the adverse effect of the impurities on subsequent conversion is reduced; finally, cellulose nanocrystals and a hydrochloric acid solution are added, on the basis that extraction is further promoted, protopanoxadiol saponin can be efficiently and directionally converted into rare ginsenoside Rg5, meanwhile, generation of an isomeride Rk1 of the rare ginsenoside Rg5 is reduced, and then the yield and purity of the Rg5 are remarkably improved.

The invention discloses a walnut kernel skin active component enrichment method based on differential pressure blasting puffing and gradient biotransformation, and relates to the technical field of agricultural product deep processing and plant active component extraction, and the method comprises the following steps: S1, carrying out differential pressure blasting puffing treatment on dried walnut kernel skin, maintaining the pressure of the material at 0.8-1.5 MPa for 10-60 seconds, and then instantaneously releasing the pressure to normal pressure within 0.05-0.2 seconds, the porosity is increased, cell walls are broken, and the specific surface area is increased; s2, mixing the puffed walnut kernel skin and water according to a solid-liquid mass ratio of 1: 10-1: 30 to form slurry, adding a compound enzyme system containing cellulase, hemicellulase, pectinase, tannase and / or protease, and performing enzymolysis for 1-4 hours under the conditions that the temperature is 45-55 DEG C and the pH is 4.5-6.0, so that cell wall components are partially hydrolyzed and bound phenols are released; and S3, sequentially carrying out first-stage fermentation and second-stage fermentation on the materials subjected to enzymolysis.

The invention discloses a synbiotics-like feed prepared from vegetable waste polymerized vegetable cakes and a method, and relates to the field of agricultural circular economy, cellulose in vegetable waste cell walls is effectively decomposed under mild conditions through a specific catalytic decomposition enzyme combination, and part of cellulose is converted into carbon dioxide and water, so that the utilization rate of the cellulose is improved. By combining physical heat energy conduction, liquid in cell vesicles is heated to generate micro air pressure to break through residual cell walls, so that recessive water in cells is successfully converted into surface dominant water, the energy consumption in the dehydration process is greatly reduced, and the loss of dry basis substances in the resource utilization process is effectively reduced; the vegetable cake product obtained through final polymerization is rich in various nutrient substances such as crude fibers and / or dietary fibers, proteins, vitamin C and carbohydrates, the recycling rate of the vegetable waste is larger than or equal to 95%, the recycling efficiency and the additional value are remarkably improved, meanwhile, the difficulty of subsequent harmless treatment is reduced from the source, and the economic input and the treatment cost are reduced.

The invention discloses a PPE composite foam material as well as a preparation method and application thereof, and belongs to the field of high polymer materials. The PPE composite foam material comprises a foam bead inner core and a conductive shell layer, electromagnetic shielding filler of the foam bead inner core is mainly distributed in a foam hole wall area, and a first electromagnetic isolation network structure in the foam bead inner core is formed; the conductive shell layer is arranged between the inner cores of the foam beads to construct a second electromagnetic isolation network structure; meanwhile, the conductive paths in the core-shell are connected and synergistically improve the conductivity and the electromagnetic shielding performance, under the synergistic effect of the core-shell structure, even if the content of the electromagnetic shielding filler in the inner core of the foam bead is low, the PPE composite foam material can obtain excellent conductivity and electromagnetic shielding performance, foaming is facilitated, and the foaming ratio is increased.

The invention discloses a cell wall associated kinase gene VvWAKL8 for regulating and controlling texture formation of grape fruits and application thereof, the cell wall associated kinase gene VvWAKL8 is cloned from the grape fruits for the first time, the nucleotide sequence of the cell wall associated kinase gene VvWAKL8 is as shown in SEQ ID NO.1, and the coded amino acid sequence is as shown in SEQ ID NO.2. Research finds that the VvWAKL8 is specifically and highly expressed in the hard-pulp grape variety, and subcellular localization analysis shows that the protein is localized in a cell membrane; instantaneous overexpression of VvWAKL8 in grape fruits can significantly improve fruit hardness and regulate pectin metabolism related gene expression, and verifies the core function of VvWAKL8 in fruit texture formation. The research reveals that the VvWAKL8 is a key gene for regulating and controlling the grape fruit texture for the first time, a brand new gene resource is provided for molecular marker-assisted breeding of a new storage-resistant grape variety, and an important foundation is laid for deeply analyzing a cell wall signal to regulate and control a molecular mechanism of fruit development.

The application discloses a kind of fruit hydraulic conductivity and biomechanical parameter measurement method and device;The measurement method includes the following steps: step 1: sampling;Step 2: measure initial fruit water potential Ψ f0 With pressure chamber;Step 3: do fruit water absorption experiment;Step 4: measure fruit pressure-volume curve (P-V curve);Step 5: measure fruit stalk + calyx hydraulic resistance and fruit stalk hydraulic resistance, respectively Rc And Rs;Step 6: data processing.The fruit hydraulic conductivity and biomechanical parameter measurement method and device, the hydraulic conductivity affecting fruit water transport and the biomechanical parameters such as volume elastic modulus, cell wall coefficient of extension, critical point swelling pressure and other influencing factors affecting cell elastoplastic deformation are conveniently obtained, which is convenient for studying fruit swelling growth, and helps to deeply understand the physiological mechanism of fruit cell swelling and its response law to changes in different habitat elements.

The use of an LtPCL6 protein and a gene encoding same in regulating pathogenicity, mycelial growth, and stress resistance of Lasiodiplodia theobromae. The gene encoding LtPCL6 protein is derived from Lasiodiplodia theobromae, and a knockout mutant is obtained by constructing a knockout fragment of a Lasiodiplodia theobromae LtPCL6 gene, and introducing same into a Lasiodiplodia theobromae protoplast. Experiments indicate that the LtPCL6 gene has an effect in the growth, cell wall and cell membrane integrity, and stress resistance of Lasiodiplodia theobromae, and significantly reduces the pathogenicity of the Lasiodiplodia theobromae with regard to a host. Therefore, the LtPCL6, serving as a fungicide target and a key protein of a pathogenic mechanism of diseases, can be used for developing agents for diseases caused by Lasiodiplodia theobromae infection and breeding new resistant varieties, and has broad application prospects in the control of plant pathogenic fungal diseases.