1596 results about "Cell wall" patented technology

A pickleball paddle includes a handle, and a paddle head coupled with the handle. The paddle head includes a first outer faceplate, a second outer faceplate, and a core arranged between the first outer faceplate and the second outer faceplate. The core includes a honeycomb structure having multiple cells, with each of the multiple cells defined by honeycomb cell walls, and each of the honeycomb cell walls having a height. A majority of the multiple cells of the honeycomb structure are filled with a polymer, the polymer does not extend past the height of the honeycomb cell walls, and the polymer does not expand when exposed to heat.

A regenerative filter that includes a filter housing having inlet and outlet zones; a fluid path provided between the inlet and outlet zones; a plurality of filter elements each having an outer surface filter media applied thereto and functioning to filter particulate or contaminants from the fluid path; and a tube sheet that is supported across the filter housing, that is disposed just before the outlet zone and that provides the support for the plurality of filter elements. The plurality of filter elements are disposed in an array and includes bridging members or elements that connect between adjacent filter elements, and that forms with the filter elements, a closed interstitial space between adjacent filter elements for liquid flow; a nanoscale barrier on interior surfaces, including that of the regenerative media itself, providing a mechanism to disrupt the cell wall of microscopic viruses and organisms.

A regenerative filter that includes a filter housing having inlet and outlet zones; a fluid path provided between the inlet and outlet zones; a plurality of filter elements each having an outer surface filter media applied thereto and functioning to filter particulate or contaminants from the fluid path; and a tube sheet that is supported across the filter housing, that is disposed just before the outlet zone and that provides the support for the plurality of filter elements. The plurality of filter elements are disposed in an array and includes bridging members or elements that connect between adjacent filter elements, and that forms with the filter elements, a closed interstitial space between adjacent filter elements for liquid flow; a nanoscale barrier on interior surfaces, including that of the regenerative media itself, providing a mechanism to disrupt the cell wall of microscopic viruses and organisms.

The invention discloses a genetically engineered algae capable of resisting aquatic pathogenic bacteria as well as a construction method and application thereof, and is characterized in that the genetically engineered algae is phaeodactylum tricornutum into which cell wall energizing plasmids pPha-T1-J48054-eGFP-Linker-Q4 or pPha-T1-J48054-eGFP-Linker-Q6 are introduced, and the preparation method of the genetically engineered algae comprises the following steps: taking genome DNA of the phaeodactylum tricornutum as a template; the method comprises the following steps: constructing a directional expression plasmid pPha-T1-J48054-eGFP by amplifying a Phatr3J48054 coding sequence, inserting Q4 and Q6 coding sequences into the downstream of the eGFP of a directional expression vector, constructing a cell wall energizing plasmid, electrically transforming the cell wall energizing plasmid into phaeodactylum tricornutum, coating an f / 2 plate culture medium with transformed phaeodactylum tricornutum cells for culturing, screening positive algae strains, and screening the positive algae strains. The feed additive has the advantage of inhibiting or killing aquatic pathogenic bacteria.

The invention discloses a method for extracting astaxanthin by using a biological enzyme, which comprises the following steps of: decomposing cell walls of haematococcus pluvialis at a certain temperature by using the biological enzyme, releasing astaxanthin from the haematococcus pluvialis, dissolving out the astaxanthin by using a certain solvent, and separating and purifying to obtain powdery astaxanthin. According to the method disclosed by the invention, wall breaking is carried out by combining ultrasound and biological enzyme, and a mixed solvent of ethanol and Tween 80 is adopted, so that the extraction rate of astaxanthin reaches 98.1%, the wall breaking time is shortened, and the efficiency is improved.

The invention discloses a tomato fruit cracking key regulation gene identification method, through the method, 41 key genes for regulating tomato cracking are determined and obtained for the first time, and the genes mainly participate in plant hormone synthesis, cell wall metabolism, wax and cuticle metabolism, absorption and transportation of mineral elements and water, and some transcription factors. Two regulation networks are established and comprise an ethylene-cell wall metabolic pathway and a transcription factor-water channel-mineral element. The research combines high-throughput sequencing and molecular biological techniques, enriches the cracking mechanism of tomato fruits on transcription and translation levels, and thus, has important scientific significance and application prospects.

The invention discloses an ulva lactuca composite wall-breaking enzymolysis fermentation product as well as a preparation method and application thereof, and relates to the field of cosmetics. The preparation method comprises the following steps: soaking ulva powder, and performing physical cell wall breaking treatment; mixed enzymes are added for an enzymolysis reaction, wherein the mixed enzymes comprise lipase, cell wall breaking enzyme, enzyme and protease; adding the enzymolysis product into a culture medium, and inoculating bacterial liquid for fermentation culture to obtain fermentation liquid. According to the application, active ingredients such as macromolecular polysaccharide and protein in the ulva lactuca are extracted and decomposed by adopting physical cell wall breaking, composite enzymolysis and bifidus yeast fermentation technologies, and the prepared fermentation product is rich in various composite micromolecular active ingredients such as oligosaccharide, polypeptide, amino acid, polyphenols and flavonoids, has high free radical scavenging capacity, and can be used for preparing the ulva lactuca beverage. The anti-wrinkle and anti-oxidation composition has good anti-wrinkle and anti-oxidation effects, is beneficial to skin absorption when being applied to cosmetics and has high absorption efficiency, and the prepared cosmetics have excellent anti-wrinkle, anti-oxidation, soothing and repairing effects.

The invention discloses a step-by-step targeting placenta trophoblast micro-nano robot as well as a preparation method and application thereof. The micro-nano robot comprises a yeast cell wall of a bag-shaped structure, bio-enzyme unevenly distributed on the surface of the yeast cell wall and targeted placenta trophoblast drug-loaded nanoparticles located in the yeast cell wall. The surfaces of the targeted placenta trophoblast drug-loaded nanoparticles are connected with placenta-like chondroitin sulfate A binding polypeptides. Inflammation placenta tissue can be targeted through macrophages, the medicine is accurately delivered to placenta trophoblastic cells through the medicine-carrying nano-particles connected with the targeting peptide, step-by-step accurate targeting is achieved, the medicine does not penetrate through the placenta barrier to reach the body of a fetus, and high biological safety is achieved for pregnancy administration.

The invention discloses an algicidal bacterium and application thereof, the algicidal bacterium is named as Bacillus sp.L1.3, and is preserved in China Center for Type Culture Collection on March 31, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 2025629. The nucleotide sequence of the 16S rRNA gene of the strain is as shown in SEQ ID NO: 1. The invention also provides application of the algicidal bacteria in treatment of water bloom of freshwater cyanobacteria, and the freshwater cyanobacteria is microcystis aeruginosa. Fermentation liquor of the strain L1.3 has a strong inhibition effect on growth of microcystis aeruginosa, secretions of the strain L1.3 can destroy cell walls, cell membranes, thylakoid and other structures of algae cells, inhibit photosynthesis of algae and cause irreversible damage to the algae, and the strain L1.3 has wide-range temperature stability and can be used for preparing the microcystis aeruginosa strain L1.3. The bacterial strain is suitable for being prepared into an algicidal bacterial agent to be applied to the field of biological control of freshwater cyanobacterial bloom.

The invention relates to a curcumin-loaded MMP response type melittin nano-pellicle vesicle as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations. The preparation method comprises the following steps: firstly, preparing curcumin entrapped lipidosome; then carrying out culture amplification on engineering bacteria carrying melittin recombinant plasmids, extracting cell membranes after removing cell walls, and carrying out ultrasonic treatment and membrane extrusion to obtain melittin cell membrane nano-vesicles; fusing the curcumin lipidosome and the cell membrane nano-vesicles in an ultrasonic extrusion mode to obtain fused vesicles; and finally, carrying out surface calcium phosphate mineralization treatment on the fused vesicles to obtain the curcumin-loaded MMP response type melittin nano pellicle vesicles. The nano mycofilm vesicle prepared by the invention can be used for preparing antitumor drugs, and the biocompatibility and in-vivo stability of nanoparticles are improved by introducing a calcium phosphate coating; through combined delivery of melittin and curcumin, the anti-tumor effect is enhanced, so that the growth and proliferation of tumor cells are inhibited.

The invention relates to the technical field of astragalus polysaccharide, in particular to an extraction method of astragalus polysaccharide. An extraction method of astragalus polysaccharide comprises the following steps: primary water extraction, secondary water extraction, vacuum concentration, primary alcohol precipitation, secondary alcohol precipitation and purification, specifically, the primary water extraction comprises the following steps: soaking astragalus with a weakly alkaline solution, heating until boiling, extracting, and filtering to separate primary precipitate and primary extract; the weakly alkaline solution is used in the primary water extraction step, so that cell walls are damaged, the solubility of the polysaccharide is improved, the release of the polysaccharide is promoted, and the extraction rate of the astragalus polysaccharide is remarkably improved.

The invention relates to the technical field of mechanical metamaterials, in particular to a negative Poisson's ratio cell element with an embedded symmetrical broken subcell structure, which comprises a concave hexagonal main body, the concave hexagonal main body comprises two horizontal cell walls and inclined cell walls arranged at two ends of the two horizontal cell walls, and the central points of the two inclined cell walls are bent towards one side close to the central points of the horizontal cell walls; the number of the bent wall rods is two, and the two bent wall rods are symmetrically arranged in the inwards-concave hexagonal body. The inherent contradiction that the stretching performance must be sacrificed when the rigidity of a traditional concave honeycomb structure is improved is successfully solved, and the negative Poisson's ratio effect and the energy absorption capacity of the structure can be further improved while the structural rigidity is enhanced.

The invention relates to the technical field of plant polysaccharide extraction, in particular to a process for synergistically extracting rape pollen polysaccharide by a compound enzyme, which comprises the following steps: crushing rape pollen to 200-300 meshes by pulse airflow, performing microwave-ultrasonic degreasing by using a 85% ethanol solution, mixing with an acetic acid-sodium acetate buffer solution with the pH value of 4.8, and performing ultrasonic extraction to obtain a crude extract; the method comprises the following steps: adding a compound enzyme system which is immobilized by Fe3O4 (at) SiO2 nanoparticles by cellulase, pectinase, protease and beta-glucosidase according to a mass ratio of 2: 1: 1: 0.5, carrying out enzymolysis for 4 hours in a 50 DEG C constant-temperature water bath and a 15V / cm pulsed electric field, carrying out enzyme deactivation on enzymatic hydrolysate, carrying out gradient ethanol precipitation, carrying out ultrafiltration deproteinization, and carrying out vacuum pulsation freeze drying to obtain the polysaccharide. According to the method disclosed by the invention, the cell wall breakage rate and the enzymolysis efficiency are improved through pulsed airflow crushing, magnetic nano immobilized enzyme and pulsed electric field assistance, the polysaccharide yield reaches 7.32%, the purity is 91%, the beta-glucosidic bond proportion and the antioxidant activity are remarkably improved, the enzyme can be recycled and reused, the drying time is shortened, and efficient and low-consumption extraction is realized.

The invention belongs to the technical field of microbiology and agricultural application thereof, and particularly relates to bacillus aerosus, a microbial agent and application of the microbial agent. According to the invention, a strain with good antagonism on tobacco black shank and fusarium root rot is separated and screened from tomato roots, and is identified as aerobacillus, and chitinase, cellulase and protease are secreted to dissolve the cell wall structure of pathogenic bacteria hyphae and inhibit the growth of pathogenic bacteria, so that the pathogenic bacteria lose the infection ability, and the pathogenic bacteria are effectively prevented from being infected. The biocontrol microbial inoculant has the antibacterial effect on more than 10 plant pathogenic fungi, the antibacterial rate is greater than 80%, especially the antibacterial rate on phytophthora nicotianae and fusarium oxysporum is greater than 90%, and pot experiments and field experiments prove that the biocontrol microbial inoculant has the control effect on tobacco black shank and fusarium root rot obviously superior to those of commercially available biocontrol microbial inoculants and chemical agents; meanwhile, the tobacco seed germination can be promoted, the growth and development of field tobacco plants are promoted, the subculture stability is better, and the application prospect is wide.

The invention belongs to the technical field of biological medicine, and particularly relates to red ginseng fermentation broth and a preparation method and application thereof.The preparation method of the red ginseng fermentation broth comprises the following steps that red ginseng powder is dissolved in water, and a red ginseng fermentation medium is obtained by breaking cell walls in red ginseng; mixing the seed solution of the plant lactobacillus and the seed solution of the fermentation mucus lactobacillus to obtain a compound strain seed solution; inoculating the compound strain seed solution into a red ginseng fermentation culture medium for fermentation culture to obtain a fermentation solution; centrifuging the fermentation liquor, collecting supernate, extruding and filtering the supernate, and collecting filtrate, so as to obtain the red ginseng fermentation liquor. According to the invention, compound strain fermentation is adopted, and a mechanical extrusion mode is combined to increase the content of total saponins of ginseng. According to the method disclosed by the invention, the concentration of the total saponins of ginseng is increased from 2.19 mg / mL to 7.53 mg / mL.

The invention discloses a tissue culture method of a double-petal hemerocallis fulva scapes, which comprises the following steps: taking a double-petal hemerocallis fulva scapes, taking a growing point as a center, transversely cutting into long sections which are not less than 5cm, washing and disinfecting; putting the disinfected long segments into enzymatic hydrolysate for cell wall enzymolysis, taking out the long segments after enzymolysis is completed, and washing the long segments with sterile water; transversely cutting the long section subjected to enzymolysis into small sections of 1-2cm by taking the growing point as the center; and carrying out tissue culture by taking the small sections as explants. The method applies the double-petal hemerocallis fulva variety, and has the advantages that materials can be taken at any time in the flowering phase, the tissue culture efficiency is high, and the quality is good.

This invention relates to the field of water treatment technology, specifically to a reverse osmosis membrane cleaning agent composed of a non-oxidizing bactericide and an enzyme, and its application. Its main purpose is to remove biofouling from reverse osmosis membranes and restore membrane flux. This patent discloses a novel reverse osmosis membrane cleaning agent formulation. Considering the different pH requirements of the cleaning agent in actual production, different types of bioactive hydrolytic enzymes are selected to disrupt the structure of bacterial cell walls and their extracellular polymers. A reducing agent and a non-oxidizing bactericide are combined to enhance the cleaning effect of the reverse osmosis membrane. For different cleaning purposes, non-oxidizing bactericides suitable for acidic and alkaline cleaning are formulated. Sodium benzoate and benzalkonium chloride show good application prospects in the removal of reverse osmosis membrane fouling.

The invention discloses a sludge treatment method and system, and relates to the field of solid waste treatment, and the method specifically comprises the following operation steps: S1, multi-frequency resonance pretreatment: destroying a sludge colloid structure through stepped mechanical vibration: collapsing an extracellular polymer network at a low frequency, breaking down microbial cell walls at an intermediate frequency to release intracellular water, pathogenic bacteria are inactivated through a high-frequency cavitation effect; s2, performing electrochemical in-situ flocculation; s3, gradient pressure dehydration; s4, low-temperature mineralization stabilization; and S5, vacuum condensation and recovery. According to the sludge treatment method and system, through stepped low-frequency, medium-frequency and high-frequency mechanical resonance, the colloid structure of the sludge can be directionally damaged, the fluidity of the sludge is remarkably improved, chemical conditioners are remarkably reduced, agent residues and secondary pollution are avoided, pathogens are physically inactivated through the high-frequency cavitation effect, and the treatment effect of the sludge is improved. Micro-nano oxygen bubbles are synchronously generated to strengthen free radical oxidation, and a sterile environment is created for subsequent mineralization.

The invention discloses a separation and purification method for improving protein purity and emulsifying property of fusarium venetii based on a cell wall simplification engineering strategy, and belongs to the technical field of food processing and protein separation and purification. The method for separating and purifying the Fusarium venanii protein comprises the following steps: dispersing Fusarium venanii fermented hyphae in water, and adjusting the pH value to 10-12 to obtain a Fusarium venanii solution; performing high-pressure homogenization, collecting supernate, adjusting the pH value to 4.4-4.5, performing centrifugation, and taking protein precipitate; redissolving the protein precipitate in water, adding chitinase for reaction, carrying out acid precipitation again after the reaction is finished, centrifuging, and taking the protein precipitate; and redissolving the protein precipitate in water, adjusting the pH value, dialyzing and drying to obtain the fusarium venanii protein. The fusarium protein obtained by the method is high in purity, has the characteristics of higher solubility, high interfacial activity, emulsifying property and the like, and is beneficial to food industrial application of the fusarium protein.

The invention discloses methanogen lyase homologous with a PeiR lyase sequence and application of the methanogen lyase, and aims to solve the problem that PeiR lyase protein of a methanogen cell wall peptide bond can participate in a biological process of hydrolyzing archaea cell walls, effectively kill methanogen and reduce methane production, and protein homologous with the PeiR lyase protein has potential methane reduction potential. According to the invention, a series of biological information software is utilized to identify protein sequences coded by methanogens virus from rumen microbiome sequencing data, and sequence homology between the proteins and PeiR protein is further analyzed to obtain a series of methanogens lyases. In order to realize the expression of the lyase, multiple segments of primers are designed to synthesize a target sequence, and the target sequence is successfully expressed in a prokaryotic expression system. Through the detection of crude enzyme liquid, the effectiveness of the lyase in the aspect of reducing the methane yield is verified in an in-vitro gas production test.

The invention relates to a nickel-free hole sealing agent and a preparation method thereof, and belongs to the technical field of high polymer materials. The nickel-free hole sealing agent comprises the following components in parts by weight: 3-10 parts of cobalt acetate, 1-11 parts of a corrosion-resistant modifier, 1-6 parts of ethylenediamine tetraacetic acid, 3-7 parts of sodium silicate and 5-15 parts of deionized water, an intermediate product containing bis-imidazoline in the corrosion-resistant modifier is tightly adsorbed on the metal surface by virtue of electrostatic attraction and a large pi bond to form a tough protective film, so that a water-phase corrosive medium is effectively isolated, the corrosion speed is remarkably reduced, and meanwhile, the corrosion-resistant modifier has good heat-resistant stability and still has a corrosion inhibition effect in a high-temperature environment; the glycine enables the nickel-free hole sealing agent to better fill tiny holes in the metal surface, so that the surface is flat and smooth, and uniform distribution and permeation of the hole sealing agent are promoted; quaternary ammonium salt destroys cell walls and cell membranes of microorganisms and inhibits growth and reproduction of the microorganisms; the nickel-free hole sealing agent prepared by the invention not only has a good antistatic effect, but also has excellent high-temperature-resistant and antibacterial effects.

The invention discloses a cooking method of a pressure cooker, which comprises the following steps: a boosting cooking stage: starting a heating device to enable the cooking pressure of the pressure cooker to reach a first preset cooking condition; in the variable-pressure wall breaking stage, a heating device is turned off, a cooling device is turned on to enable the cooking pressure of the pressure cooker to be rapidly reduced from the first preset cooking condition to a second preset cooking condition, so that cell walls of cooking food materials are broken under the action of pressure difference formed in a short time, and the second preset cooking condition at least comprises that the cooking pressure is larger than normal pressure. When the cooking pressure is rapidly reduced from the first preset cooking condition to a second preset cooking condition, the rapid pressure reduction rate is at least 1.8 times of the natural pressure reduction rate. Through the method, food materials which are not easy to cook and have thick cell walls can be cooked in a short time, meanwhile, nutrient substances are fully dissolved out, and the cooking taste is improved.

The present disclosure relates to compositions and methods comprising a yeast cell wall extract and a clay material admixed to a proteinaceous and / or cell wall extract(s) from bacterial or fungal fermentation from amino acid production. In another embodiment, the present compositions comprise a yeast cell wall extract, a clay material, and an algal material admixed to a proteinaceous and / or cell wall extract(s) from bacterial or fungal fermentation from amino acid production. This disclosure also relates to methods of utilizing the same to sequester and / or adsorb mycotoxins internal or external to an animal, thereby reducing and / or preventing the toxic and harmful effects of mycotoxin contamination and / or infection in animals. Specifically, application of the present methods and compositions are found in the dietary administration and / or co-administration of the present compositions to animals in animal feed and / or animal production systems, as well as in therapeutic, prophylactic, and research applications.

The invention belongs to the technical field of saccharomyces cerevisiae beta-glucan, and relates to a preparation method of saccharomyces cerevisiae-sourced yeast beta-glucan. The invention relates to a preparation method of saccharomyces cerevisiae-sourced yeast beta-glucan, which comprises the following steps: uniformly mixing a yeast raw material with a first solvent, and carrying out high-pressure homogenization to obtain a homogenized feed liquid; adding food-grade alkali into the homogenized feed liquid to carry out extraction reaction, centrifuging the obtained reaction extracting solution, and separating a heavy phase to obtain a heavy-phase insoluble cell wall extract; and continuously adding a second solvent for resuspension, adding a compound enzyme preparation, adjusting the pH value of the reaction system by using an acidic reagent, carrying out an enzymatic hydrolysis reaction, centrifuging after the reaction is finished, recovering a heavy phase, and drying to obtain the insoluble yeast beta-glucan dry powder. According to the preparation method of the yeast glucan, provided by the invention, the conventional preparation process flow is shortened, the environmental protection treatment pressure is greatly relieved, the production cost is effectively reduced, and the prepared yeast glucan is higher in purity and has a better industrial application prospect.

The invention discloses a method for synergistically preparing recycled aggregate from urban renewal garden waste and building waste, and relates to the field of treatment of garden waste and building waste. The method comprises the following steps: carrying out molecular-level deconstruction pretreatment on garden wastes, destroying cell walls by adopting dual-frequency ultrasound combined with biological enzymolysis, and treating the building wastes by combining high-pressure water jet with abrasive impact; performing nano-scale composite granulation, adding a nano-composite binder, and introducing CO2 to form a gel transition layer; by means of plasma-assisted sintering, particle diffusion is accelerated by means of radio-frequency plasma and an electric field; chemical vapor deposition is adopted, and a TiN-TiO2 gradient functional coating is formed; real-time monitoring and regulation are performed through a multi-mode sensor network and an intelligent algorithm, and process parameters are adjusted according to a product performance prediction result. The recycled aggregate disclosed by the invention is high in strength, good in heat preservation and strong in freezing resistance; negative carbon emission reduction is achieved in the production process, an intelligent system guarantees stable product quality, environmental protection and economic benefits are achieved, and urban update and solid waste treatment green transformation are promoted.

A method of making a vegetable- and / or fruit-containing snack food is described. The snack food includes a rigid starch matrix that contains potato starch and a plurality of individual pieces of vegetable and / or fruit randomly distributed throughout the matrix. At least 50% by number of the pieces have a minimum dimension of at least 0.75 mm and a maximum dimension of up to 7 mm. The matrix includes a first cellular structure of first cellular pores having a first pore size distribution and the pieces include a second cellular structure of second cellular pores having a second pore size distribution. At least some of the second cellular pores are defined by polysaccharide cell walls of the respective vegetable and / or fruit. The first pore size distribution has a larger number-average pore size and a wider distribution than the second pore size distribution.

According to the elm root extract and the extraction method and application thereof, ultrasonic-assisted staged enzymolysis is adopted, elm roots are decomposed through the special activity of various enzymes, cells are broken, and the material transfer barrier between cell walls and cells is reduced, so that the purpose of extracting effective active ingredients of polyphenol and polysaccharide in the elm roots to the maximum extent is achieved. According to the invention, the effective active ingredients in the elm root extract are increased in a targeted manner, and it is proved that the elm root extract has an anti-inflammatory effect and also has an anti-skin aging cosmetic application, especially a tightening effect, and has a good application prospect in cosmetic compositions. The composition can be applied to personal care products with anti-aging and firming effects.

The invention relates to the technical field of new materials, and discloses an antibacterial chitosan modified polypropylene fiber, isothiazolinone is used as a heterocyclic compound, the antibacterial mechanism of the antibacterial chitosan modified polypropylene fiber is mainly non-oxidized antibacterial, and a hydrogen bond is formed by a heterocyclic ring and a basic group of DNA in bacteria, so that the DNA structure is destroyed, and the antibacterial and bacteriostatic effects are achieved; a cationic quaternary ammonium salt antibacterial group is introduced to a chitosan molecular chain, cell membranes and cell walls of bacteria can be destroyed through the gravitation effect of an electric field, so that the antibacterial effect is achieved, and under the synergistic effect of the two antibacterial functional groups, the sterilization and bacteriostasis effects on most bacteria on the surface of the fabric can be achieved; meanwhile, due to the introduction of chitosan, hydrophilic groups such as residual hydroxyl groups and amino groups on the chitosan can improve the dyeing performance of the polypropylene and play a role in assisting dyeing of a finishing agent, and a fabric made of the antibacterial polypropylene fiber can have a more long-acting and lasting antibacterial performance.

The invention discloses a method for extracting PN / PDRN from lactobacillus bulgaricus and application of the PN / PDRN in relieving and anti-inflammatory aspects, belongs to the technical field of biochemistry, and solves the problems that in the prior art, the acquisition source of PN / PDRN is unstable, and pollution easily exists. According to the preparation method, cell walls of lactobacillus bulgaricus are preliminarily dissolved through alkaline conditions and lysozyme in the preparation process, cell membranes of bacteria are destroyed through lauryl sodium sulfate to be split, the conditions are mild, meanwhile, PVP is added to protect a PN structure, and the purity of the PN / PDRN is effectively improved through a final product prepared through the preparation method.

The invention discloses methanogen lyase homologous with high-dimensional characteristics of PeiW lyase and application of the methanogen lyase, and aims to solve the problem that PeiW lyase protein of methanogen cell wall peptide bonds can participate in the biological process of hydrolyzing archaea cell walls, effectively kill methanogen and reduce methane production, and protein homologous with the protein has potential methane reduction potential. According to the method, a series of bioinformatics software is utilized, a protein sequence coded by methanogens virus is identified from a large amount of rumen microorganism virus group sequencing data, the high-dimensional homology of a deep learning model between the protein sequence and PeiW is analyzed, and two methanogens lyases similar to the high-dimensional characteristics obtained by the PeiW model are obtained. In order to realize the expression of the lyase, multiple segments of primers are designed to synthesize a target sequence, and the target sequence is successfully expressed in a prokaryotic expression system. Through detection of a crude enzyme solution, the effectiveness of the lyase in the aspect of reducing the yield of methane is verified in an in-vitro gas production test.