904 results about "Cell wall" patented technology

The invention discloses a method for extracting astaxanthin by using a biological enzyme, which comprises the following steps of: decomposing cell walls of haematococcus pluvialis at a certain temperature by using the biological enzyme, releasing astaxanthin from the haematococcus pluvialis, dissolving out the astaxanthin by using a certain solvent, and separating and purifying to obtain powdery astaxanthin. According to the method disclosed by the invention, wall breaking is carried out by combining ultrasound and biological enzyme, and a mixed solvent of ethanol and Tween 80 is adopted, so that the extraction rate of astaxanthin reaches 98.1%, the wall breaking time is shortened, and the efficiency is improved.

The invention relates to a curcumin-loaded MMP response type melittin nano-pellicle vesicle as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations. The preparation method comprises the following steps: firstly, preparing curcumin entrapped lipidosome; then carrying out culture amplification on engineering bacteria carrying melittin recombinant plasmids, extracting cell membranes after removing cell walls, and carrying out ultrasonic treatment and membrane extrusion to obtain melittin cell membrane nano-vesicles; fusing the curcumin lipidosome and the cell membrane nano-vesicles in an ultrasonic extrusion mode to obtain fused vesicles; and finally, carrying out surface calcium phosphate mineralization treatment on the fused vesicles to obtain the curcumin-loaded MMP response type melittin nano pellicle vesicles. The nano mycofilm vesicle prepared by the invention can be used for preparing antitumor drugs, and the biocompatibility and in-vivo stability of nanoparticles are improved by introducing a calcium phosphate coating; through combined delivery of melittin and curcumin, the anti-tumor effect is enhanced, so that the growth and proliferation of tumor cells are inhibited.

This invention relates to the field of water treatment technology, specifically to a reverse osmosis membrane cleaning agent composed of a non-oxidizing bactericide and an enzyme, and its application. Its main purpose is to remove biofouling from reverse osmosis membranes and restore membrane flux. This patent discloses a novel reverse osmosis membrane cleaning agent formulation. Considering the different pH requirements of the cleaning agent in actual production, different types of bioactive hydrolytic enzymes are selected to disrupt the structure of bacterial cell walls and their extracellular polymers. A reducing agent and a non-oxidizing bactericide are combined to enhance the cleaning effect of the reverse osmosis membrane. For different cleaning purposes, non-oxidizing bactericides suitable for acidic and alkaline cleaning are formulated. Sodium benzoate and benzalkonium chloride show good application prospects in the removal of reverse osmosis membrane fouling.

The invention discloses a cooking method of a pressure cooker, which comprises the following steps: a boosting cooking stage: starting a heating device to enable the cooking pressure of the pressure cooker to reach a first preset cooking condition; in the variable-pressure wall breaking stage, a heating device is turned off, a cooling device is turned on to enable the cooking pressure of the pressure cooker to be rapidly reduced from the first preset cooking condition to a second preset cooking condition, so that cell walls of cooking food materials are broken under the action of pressure difference formed in a short time, and the second preset cooking condition at least comprises that the cooking pressure is larger than normal pressure. When the cooking pressure is rapidly reduced from the first preset cooking condition to a second preset cooking condition, the rapid pressure reduction rate is at least 1.8 times of the natural pressure reduction rate. Through the method, food materials which are not easy to cook and have thick cell walls can be cooked in a short time, meanwhile, nutrient substances are fully dissolved out, and the cooking taste is improved.

The present disclosure relates to compositions and methods comprising a yeast cell wall extract and a clay material admixed to a proteinaceous and / or cell wall extract(s) from bacterial or fungal fermentation from amino acid production. In another embodiment, the present compositions comprise a yeast cell wall extract, a clay material, and an algal material admixed to a proteinaceous and / or cell wall extract(s) from bacterial or fungal fermentation from amino acid production. This disclosure also relates to methods of utilizing the same to sequester and / or adsorb mycotoxins internal or external to an animal, thereby reducing and / or preventing the toxic and harmful effects of mycotoxin contamination and / or infection in animals. Specifically, application of the present methods and compositions are found in the dietary administration and / or co-administration of the present compositions to animals in animal feed and / or animal production systems, as well as in therapeutic, prophylactic, and research applications.

The invention belongs to the technical field of saccharomyces cerevisiae beta-glucan, and relates to a preparation method of saccharomyces cerevisiae-sourced yeast beta-glucan. The invention relates to a preparation method of saccharomyces cerevisiae-sourced yeast beta-glucan, which comprises the following steps: uniformly mixing a yeast raw material with a first solvent, and carrying out high-pressure homogenization to obtain a homogenized feed liquid; adding food-grade alkali into the homogenized feed liquid to carry out extraction reaction, centrifuging the obtained reaction extracting solution, and separating a heavy phase to obtain a heavy-phase insoluble cell wall extract; and continuously adding a second solvent for resuspension, adding a compound enzyme preparation, adjusting the pH value of the reaction system by using an acidic reagent, carrying out an enzymatic hydrolysis reaction, centrifuging after the reaction is finished, recovering a heavy phase, and drying to obtain the insoluble yeast beta-glucan dry powder. According to the preparation method of the yeast glucan, provided by the invention, the conventional preparation process flow is shortened, the environmental protection treatment pressure is greatly relieved, the production cost is effectively reduced, and the prepared yeast glucan is higher in purity and has a better industrial application prospect.

The invention relates to the technical field of scalp care, in particular to a hair-blacking composition for promoting melanin secretion and a preparation method of the hair-blacking composition. Comprising the following components in parts by weight: water, ethanol, a ginseng root extract, a polygonum multiflorum extract, a cacumen biotae extract, a licorice root extract, an eclipta prostrata extract, a ganoderma atrum extract, a rhizoma bletillae root extract, a mulberry leaf extract, radix sophorae flavescentis powder, a glossy privet fruit extract, a raspberry fruit extract and a black sesame extract. According to the technical scheme, the ganoderma atrum extract is prepared through enzymolysis-ultrasonic combined modification, a cell wall structure is decomposed through compound enzyme, then cell remains are further broken through the ultrasonic cavitation effect, and the release rate of active peptide and polysaccharide is increased; the hair-blacking composition can promote the generation of hair melanin so as to blacken hair, improve the hair quality, enable the hair to become tough and elastic, and enable the hair to gradually become black, smooth and firm after being smeared on the scalp for a long time.

The invention discloses a vertical differential pressure sterilizer and a sterilization method thereof, and belongs to the technical field of medical instrument sterilization equipment. The invention aims to solve the problems of long sterilization period and possible damage to effective components of materials in the existing sterilization technology. According to the technical scheme, the vertical differential pressure sterilizer comprises a vertical three-layer barrel (an inner barrel, a steam interlayer and a heat preservation layer), a vacuum pump set, a straight-through steam control valve set, a jacket steam control valve set and a jacket cooling control valve set. The inner cylinder is firstly vacuumized, then the steam interlayer and the straight steam are used for heating materials together, and the steps of vacuumizing and steam filling are repeated for multiple times, so that cell wall structures of microorganisms are damaged under the alternate action of vacuum and pressure, and rapid sterilization is realized at a relatively low temperature. And after sterilization, cooling water is injected into the steam interlayer to realize rapid cooling. The device has the advantages of being compact in structure, rapid in sterilization, energy-saving, environment-friendly and capable of effectively protecting material components.

The present invention provides an aqueous polyhydroxyalkanoate coating, a preparation method therefor, and a use thereof. The preparation method for the aqueous polyhydroxyalkanoate coating comprises: subjecting a polyhydroxyalkanoate fermentation solution to cell wall breaking and extraction, to obtain an aqueous polyhydroxyalkanoate dispersion, and then mixing the aqueous polyhydroxyalkanoate dispersion with an additive to obtain the aqueous polyhydroxyalkanoate coating, a dispersant and talcum powder being added during the cell disruption process. By means of using talcum powder to replace a portion of a dispersant as a protective additive for PHA particles, the present invention can prevent the aggregation of PHA particles at high purity, and the resulting aqueous PHA coating exhibits good coating water resistance.

This invention discloses a method for extracting PN / PDRN from Lactobacillus bulgaricus and its application in soothing and anti-inflammatory effects. Belonging to the field of biochemistry, this method solves the problems of unstable PN / PDRN sources and susceptibility to contamination in existing technologies. In this invention, the preparation relies on alkaline conditions and lysozyme to initially dissolve the cell wall of Lactobacillus bulgaricus, and then sodium dodecyl sulfate is used to disrupt the bacterial cell membrane, causing it to lyse. The conditions are mild, and PVP is added to protect the PN structure. The final product prepared by this invention effectively improves the purity of the obtained PN / PDRN.

The invention relates to the technical field of biological materials, in particular to a pH response collagen microneedle patch loaded with antibacterial nanoparticles as well as a preparation method and application of the pH response collagen microneedle patch loaded with the antibacterial nanoparticles, and the microneedle patch is prepared by loading the antibacterial nanoparticles, aldehyde modified F127 and recombinant three-type collagen COL3 on a polymer matrix. When the microneedle patch is applied to a chronic wound of a diabetic patient, the microneedle penetrates through the skin in a minimally invasive mode and directly reaches a wound focus area. Under the irradiation of 808nm near-infrared light, the mesoporous polydopamine absorbs light energy, so that the loaded lyase LysAB2 is quickly released, bacterial cell walls are specifically recognized and cracked, and bacteria are efficiently killed; meanwhile, the collagen hydrogel constructs an appropriate microenvironment at the wound, cell adhesion, proliferation and migration are promoted, and the wound healing process is accelerated. Compared with a traditional diabetes mellitus chronic bacterial infection wound treatment means, the microneedle patch provided by the invention provides an efficient and safe new strategy for treating the chronic bacterial infection wound of a diabetes mellitus patient.

The invention discloses an efficient extraction and compounding method of ergothioneine, and relates to the technical field of ergothioneine preparation, the efficient extraction and compounding method comprises the steps of raw material pretreatment, extraction, purification, compounding and embedding, raw material pretreatment adopts an enzymolysis and chelation synergistic technology, extraction adopts a pH response type aqueous two-phase system and is combined with ultrasonic microwave synergistic enhancement, and the compounding and embedding are carried out. A nanofiltration membrane separation and vacuum freeze drying combined process is adopted for purification, a targeted compounding system is adopted for compounding, and a composite wall material microcapsule embedding technology is adopted for embedding. According to the method, the cell wall structure of the raw material is destroyed and the oxidation of active ingredients is inhibited through an enzymolysis and chelation synergistic pretreatment technology, the ergothioneine distribution behavior is regulated and controlled and the mass transfer process is accelerated by matching with a pH response type aqueous two-phase system and ultrasonic microwave synergistic enhanced extraction, and then a nanofiltration membrane separation and vacuum freeze drying combined purification process is combined; impurities are effectively removed, the activity of components is reserved, and the problems of low extraction rate and insufficient purity of a traditional method are solved.

The invention relates to the technical field of polyester fibers, in particular to a preparation method of antibacterial modified polyester fibers, the prepared antibacterial modified polyester fibers have excellent antibacterial performance, a multi-layer antibacterial system is formed by introducing a nano-zinc oxide and Schiff base compound antibacterial agent into the polyester fibers, and the antibacterial performance of the polyester fibers is improved. Nano zinc oxide and copper / zinc oxide particles have broad-spectrum antibacterial property and can destroy cell walls and cell membranes of bacteria so as to achieve the antibacterial effect, and Schiff base as an organic antibacterial agent can be combined with receptors on the cell membranes of the bacteria to enhance the antibacterial effect; chitosan and cinnamyl aldehyde are adopted as raw materials for preparation of the Schiff base, and the two substances have good biocompatibility and degradability, so that the Schiff base compound antibacterial agent is safer and more environmentally friendly in the fiber; the nano zinc oxide, the copper / zinc oxide particles, the chitosan, the cinnamyl aldehyde and the like in the raw materials are all environment-friendly materials and are good in degradability, and environmental pollution and ecological damage can be reduced.

The invention relates to the technical field of preparation of bath foam capable of relieving infantile eczema, and particularly discloses bath foam capable of relieving infantile eczema and a preparation method thereof.The preparation method comprises the steps that kochia scoparia fruits, schizonepeta, folium artemisiae argyi, radix sophorae flavescentis, pepper fruits, cnidium monnieri fruits, radix saposhnikoviae, polygonum aviculare and gleditsia sinensis seeds are freeze-dried and then smashed, and pretreatment powder is obtained; performing grouped extraction on the pretreated powder to obtain an extracting solution; mixing the extracting solutions step by step, and sequentially performing low-temperature aging, gradient alcohol precipitation and sterilization to obtain the bath foam capable of relieving infantile eczema. According to the method, cell walls of the raw materials are broken through low-temperature freezing by adopting a method of firstly freezing and breaking walls and then grouping extraction, and the yield of active ingredients is remarkably improved by combining the temperature gradient and solvent concentration gradient design during grouping extraction, so that the treatment effect of the traditional Chinese medicine composition on eczema is improved.

The invention relates to the technical field of hydrogel, in particular to a preparation process of antibacterial hydrophilic hydrogel and application of the antibacterial hydrophilic hydrogel in organoid tissue engineering. The preparation process comprises the following steps: S1, preparing a base material; the base material is prepared from the following raw materials in parts by weight: 300 to 400 parts of deionized water, 60 to 80 parts of acryloyloxyethyl trimethyl ammonium chloride, 40 to 60 parts of polyoxyethylene, 20 to 30 parts of antibacterial agent, 6 to 10 parts of methyl methacrylate and 1 to 3 parts of photoinitiator 1173. According to the preparation method, the contact area between the silver nanoparticles and bacteria is increased, cell membranes are damaged, silver ions are released, chitosan and beta-sodium glycerophosphate form a temperature-sensitive gel precursor after being dissolved, and due to the fact that chitosan carries positive charges, the bacteria can be adsorbed and cell walls can be damaged through the electrostatic interaction; the chitosan and the silver nanoparticles form a slow-release carrier, so that the release time of silver ions is prolonged, and the antibacterial property of the antibacterial hydrophilic hydrogel is further improved.

The invention discloses a co-fermentation method based on sphingomonas and a rhodiola rosea extracting solution and application of a product, and belongs to the technical field of microorganisms. By utilizing a special rich enzyme system of sphingomonas and combining with an early-stage composite enzymolysis process and synergistic effect, the wall breaking efficiency of rhodiola rosea cell walls and the conversion degree of active components of rhodiola rosea are greatly improved, the bottlenecks of low efficiency and low bioavailability of a traditional rhodiola rosea extraction method are successfully overcome, and the rhodiola rosea extraction method is suitable for industrial production. The functional components such as salidroside and flavone can be fully released and converted. Secondly, different from non-symbiotic bacteria (such as lactic acid bacteria and saccharomycetes) which are commonly used for fermentation in the prior art, a potential symbiotic bacterium of sphingomonas XJL-TP07 from a special environment is innovatively selected and has better metabolic suitability with the rhodiola rosea component, the fermentation process is milder and safer (no irritant is produced), and the rhodiola rosea fermentation method is suitable for large-scale production. And a synergistic interaction effect can be generated through a unique biotransformation path.

The invention belongs to the technical field of enzymolysis and fermentation of lignocellulose, and particularly relates to a method for promoting enzymolysis and fermentation of straw cellulose by using fresh straw wall protein, which comprises the following steps: firstly, extracting straw cell wall protein and culturing engineering bacteria to obtain expansive-like protein; the method comprises the following steps: mixing the fresh straw cell wall protein and the expansion-like protein with steam explosion straw, carrying out pretreatment under proper pH and concentration, carrying out cellulose enzymolysis and synchronous saccharification fermentation, and finally converting into ethanol. The method effectively improves the straw conversion efficiency, is suitable for bioethanol production, combines the fresh straw cell wall protein and the expansion-like protein from microorganisms, and improves the bioethanol yield. The expansive-like protein and the fresh straw cell wall protein have a remarkable synergistic effect, the expansive-like protein can destroy a cellulose crystal structure in a non-hydrolytic manner and increase accessibility of cellulose, and the fresh straw cell wall protein provides multiple enzyme activities to jointly promote the enzymolysis process.

The invention relates to a method for extracting genome DNA of plants rich in secondary metabolites and a buffer solution, and belongs to the technical field of molecular biology and botany. The method solves the technical problems of low DNA extraction efficiency, poor purity, easy degradation and the like when a traditional DNA extraction method is used for treating plant tissues rich in secondary metabolites such as alkaloid, polyphenol and the like. Comprising the following steps: adding a complexing agent such as polyethylene glycol or polyvinylpyrrolidone when grinding plant tissues in a liquid nitrogen environment; splitting by using a cell wall splitting buffer solution containing dithiothreitol and a nonionic surfactant; carrying out DNA release and extraction at 60-70 DEG C by adopting a CTAB (Cetyltrimethyl Ammonium Bromide) extraction buffer solution containing polyethylene glycol and papain; and then purifying and precipitating to obtain high-purity genome DNA (Deoxyribose Nucleic Acid). The method can effectively remove alkaloid, protein and other impurities, significantly improves the DNA yield and purity, and is suitable for genome sequencing, genetic resource protection, medicinal plant molecular identification and the like of plants with high secondary metabolites such as Stephania kwangsiensis and the like.

A honeycomb core, formed from a plurality of polygonal cells arranged in an array. Each polygonal cell has lateral cell walls, the lateral cell walls of each polygonal cell forming a polygonal ring, the lateral cell walls being composed of a sheet material, the sheet material being a symmetric three-layer or five-layer thermoplastic co-extruded sheet material having an inner central or core layer and outer layers. The symmetric three-layer or five-layer thermoplastic co-extruded sheet material provides improved mechanical properties compared to a simple sheet material.

The invention relates to the technical field of polysaccharide extraction, and discloses a method for extracting high-purity pectin through cooperation of pulse microwave and low temperature, which comprises the following steps: applying a microsecond-level microwave pulse sequence corresponding to inherent acoustic resonant frequency of raw material particles, and monitoring net absorption power and acoustic signals on line, so as to adaptively control frequency locking and pulse form in a closed-loop manner; according to the invention, the electromagnetic energy of microwaves is converted into instantaneous mechanical work, and efficient structural damage to the plant cell walls from inside to outside is realized through a resonant coupling effect on the premise of maintaining the low temperature of the system; the online self-adaptive closed-loop control mechanism ensures the whole-process optimization of the energy coupling efficiency and the process stability, so that the inherent contradiction that the product quality and the extraction efficiency are difficult to consider at the same time in the traditional extraction technology is solved.

The application provides a method for synchronously separating active substances of Haematococcus pluvialis, and relates to the technical field of natural product extraction, and comprises an extraction phase with a mass ratio of 20%-50%, and the balance is water; the extraction phase is a quaternary phosphonium ionic liquid or a eutectic solvent; the quaternary phosphonium ionic liquid comprises tetrabutylphosphonium trifluoroacetate or tributyl octyl phosphonium bromide; and the eutectic solvent comprises a quaternary phosphonium ionic liquid and a fatty acid with a molar ratio of 1:1-5:3. The extraction phase has weak acidity, the ability to form van der Waals forces and hydrogen bond interactions, the Haematococcus pluvialis cell wall breaking effect and high solubility. The extraction phase can form liquid-liquid phase separation, can realize the synchronous extraction of Haematococcus pluvialis cell wall breaking, astaxanthin, carbohydrates and proteins, and has no organic solvent, is simple to operate, has short extraction time and high extraction efficiency. The method solves the technical problem that the extraction agent in the prior art cannot effectively separate multiple active substances of Haematococcus pluvialis.

The invention relates to the technical field of deep processing of vegetable fat, in particular to a preparation method and application of squalene-containing macadamia nut oil. Through the synergistic effect of directional enzymolysis conversion and low-temperature step-by-step extraction and the combined effect of the three enzymes (cellulase destroys cell walls, lipase releases grease and beta-glucosidase removes inhibitors, and the three enzymes form a wall breaking-oil releasing-synergistic synergistic reaction) by utilizing a compound enzyme which is fixedly matched and proportioned. The compound enzyme can be used for synchronously treating cellulose, lipid and glycoside substances, so that the release efficiency of squalene is improved; and step-by-step extraction is utilized to reduce extraction impurities, so that the content of squalene in the macadamia nut oil can be increased, and the extraction and enrichment of squalene in the macadamia nut meat can be realized.

The invention provides a vibration noise detection device and method of a wall breaking machine, and mainly relates to the field of vibration noise detection. A vibration noise detection device of a wall breaking machine comprises a positioning mechanism arranged in a detection chamber and a plurality of noise detection mechanisms, coaxial placement and height adjustment of the wall breaking machine are achieved through clamping and positioning of the positioning mechanism, and the wall breaking machine can be located at the detection height on different workbenches. At least four noise detection mechanisms are arranged, each noise detection mechanism comprises a supporting arm, a supporting cavity is formed in the inner side of the front end of each supporting arm, a telescopic arm is arranged on each supporting cavity in a sliding mode, and a sound level meter and a distance sensor are arranged at the front end of each telescopic arm. The invention has the following beneficial effects: the invention creatively provides a structure and a method for respectively detecting noise by a plurality of workbenches, so that the noise intensity of the wall breaking machine in the daily use process can be more reasonably represented.

The invention belongs to the field of biosensing and microbiological detection, and relates to a staphylococcus aureus rapid counting method based on fluorescence confinement discretization. According to the method, oat bran is used as a natural carbon source, lysozyme active fragments are used as targeting ligands, targeting carbon quantum dots are synthesized through a one-step hydrothermal method, the targeting carbon quantum dots are modified by fluorescent dye SRB to obtain luminescence enhanced carbon quantum dots, and the luminescence enhanced carbon quantum dots can be specifically combined with cell walls of staphylococcus aureus, so that the staphylococcus aureus has stable fluorescence signals. And then, by taking the marked thalli as a core layer and a high-molecular polymer as a shell layer, preparing a core-shell nanofiber membrane by adopting a coaxial electrostatic spinning technology to realize spatial confinement and monomer discretization of the thalli, so that a group fluorescence signal is converted into a single-thalli distinguishable signal, and rapid and sensitive quantitative detection of the staphylococcus aureus is realized. The method is simple, convenient, high in sensitivity, suitable for rapid visualization and quantitative detection of staphylococcus aureus in foods such as dairy products, meat products and beverages, and wide in application prospect.

The invention relates to an extraction process of total flavonoids of gold-edge roses, and aims to solve the technical problems of low extraction rate, easiness in pyrolysis inactivation of components and poor stability in the existing extraction technology. The invention provides an enzymatic wall breaking-in-situ inclusion synergistic extraction scheme, which is technically characterized in that in a pure water system, the directional degradation effect of pectinase on a cell wall pectin matrix is utilized, and in-situ encapsulation protection of beta-cyclodextrin on dissolved active molecules is matched; enzymolysis and clathration processes are synchronously executed under a mild temperature condition, so that instantaneous steady-state locking is carried out on heat-sensitive flavone while physical wall breaking is realized. According to the process, the yield of total flavonoids is remarkably increased, oxidative degradation in the extraction process is effectively inhibited, and the obtained product is good in water solubility and physical and chemical stability. No organic solvent is needed in the whole process, conditions are mild, and a new path is provided for efficient extraction of active ingredients of the gold-edge roses.

The utility model discloses microalgae cell wall breaking equipment, and relates to the technical field of microalgae cell wall breaking equipment. The device comprises a treatment tank, a vertical rotating shaft is rotationally arranged at the middle end of the treatment tank, a plurality of stirring pieces distributed at equal intervals are fixedly arranged at the end, located in the treatment tank, of the rotating shaft, two symmetrically-arranged arc-shaped plates are arranged in the treatment tank, and a plurality of leakage grooves distributed at equal intervals are formed in the arc-shaped plates. Two long rods which are symmetrically arranged are fixedly arranged at the middle end of the arc-shaped plate; through linkage of the transfer plate, the rotating block, the swing plate, the long plate and other parts, the arc-shaped plate can push mixed raw materials at the inner edge of the treatment tank to be in continuous contact with the stirring piece, the over-static state of the conventional stirring mode is broken through, the raw materials are mixed more dynamically, the problem of long mixing time caused by insufficient mixing is avoided, and the mixing efficiency is improved. Therefore, the mixing efficiency is improved, and the problem that the treatment efficiency is affected by the mixing mode in the existing chemical wall breaking process is solved.

This invention discloses a honeycomb-structured, micro-pressure-enhanced, and efficacious plant-based soft film powder substrate and its preparation method, belonging to the field of cosmetic technology. The soft film powder substrate, by weight, comprises 15-30 parts sodium alginate, 5-12 parts calcium sulfate, 3-8 parts Tremella fuciformis polysaccharide, 20-40 parts fine-particle-size rice starch, 10-25 parts coarse-particle-size rice flour, 2-6 parts frozen-cell-broken Ophiopogon japonicus extract, and 2-6 parts frozen-cell-broken Polygonatum odoratum extract. This invention constructs a honeycomb porous framework using gradient particle size powders, optimizes the alginate-calcium ion intelligent cross-linking system, and prepares highly active plant extracts using ultra-low temperature freezing and cell wall disruption. It controllably constructs a honeycomb gel network, achieving excellent micro-pressure-enhanced penetration and moisturizing effects, while also possessing excellent thermal stability, cold storage stability, and light stability. This solves the technical problems of existing soft film powders, such as poor film-forming structure, weak penetration-enhancing effect, insufficient stability, and low utilization rate of active ingredients. The formula is mild and safe.

This application belongs to the fields of microbiology and cell biology, and provides a method for directly preparing germination-promoting fungal protoplasts from orchid protocorms, comprising: (1) physical homogenization; (2) filtration; (3) removal of plant contents and residual cell walls; (4) purification of mycelial clusters; and (5) release and purification of fungal protoplasts. Through the rational design of homogenization and purification steps, this application achieves high-yield, high-purity, and high-vitality preparation of germination-promoting fungal protoplasts, providing excellent materials for orchid gene variety breeding and genetic engineering operations.

The invention provides array hole porous graphite as well as a preparation method and application thereof, and relates to the technical field of porous graphite materials. The preparation method of the array hole porous graphite comprises the following steps: by taking biomass with an array hole structure as a raw material, removing lignin in the raw material by using a chemical reagent, and further strengthening an orientation structure of a cell wall by using a hot-pressing method, so as to obtain an orientation enhanced precursor; and further carbonizing to obtain the porous carbon skeleton. The preparation method comprises the following steps: preparing a carbon skeleton, introducing an element with a catalytic graphitization function into the carbon skeleton through impregnation, carrying out graphitization treatment under a high temperature condition to promote the graphitization degree of cellulose in a thermal conversion process, and promoting the removal of catalytic components at a high temperature by utilizing developed array holes of porous carbon, so that the catalytic performance of cellulose is improved. The array hole porous graphite with high thermal conductivity and high crystallinity is prepared. The problems that existing biological matrix array hole porous graphite is poor in crystallinity and low in heat conductivity are solved.

The invention relates to the technical field of plant culture, in particular to a culture medium for increasing the accumulation amount of secondary metabolites of a transgenic engineering material and application of the culture medium. The invention provides a culture medium for increasing the accumulation amount of plant secondary metabolites. The culture medium comprises a basic culture medium, sucrose and inositol, the concentration of sucrose in the culture medium is 5-60g / L; the concentration of inositol in the culture medium is 100 to 600mg / L; the pH value of the culture medium is 5.7 to 5.9; the basic culture medium comprises an MS liquid culture medium. The culture medium provided by the invention can improve the cell wall thickness of a culture and increase the biomass of the culture, so that the storage capacity of the culture on secondary metabolites (such as anthocyanin) is improved, the purpose of increasing the accumulation amount of the secondary metabolites is achieved, and the culture medium can be used for plant cell culture and other aspects. Compared with the traditional culture medium, the culture medium provided by the invention has the advantages of basically no difference in cost and no difference in preparation method.