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1289 results about "Cell wall" patented technology

A cell wall is a structural layer surrounding some types of cells, just outside the cell membrane. It can be tough, flexible, and sometimes rigid. It provides the cell with both structural support and protection, and also acts as a filtering mechanism. Cell walls are present in most prokaryotes (except mollicute bacteria), in algae, fungi and eukaryotes including plants but are absent in animals. A major function is to act as pressure vessels, preventing over-expansion of the cell when water enters.

Pickleball paddle

A pickleball paddle includes a handle, and a paddle head coupled with the handle. The paddle head includes a first outer faceplate, a second outer faceplate, and a core arranged between the first outer faceplate and the second outer faceplate. The core includes a honeycomb structure having multiple cells, with each of the multiple cells defined by honeycomb cell walls, and each of the honeycomb cell walls having a height. A majority of the multiple cells of the honeycomb structure are filled with a polymer, the polymer does not extend past the height of the honeycomb cell walls, and the polymer does not expand when exposed to heat.
Owner:SPARTUS LLC

Regenerative media filtration

A regenerative filter that includes a filter housing having inlet and outlet zones; a fluid path provided between the inlet and outlet zones; a plurality of filter elements each having an outer surface filter media applied thereto and functioning to filter particulate or contaminants from the fluid path; and a tube sheet that is supported across the filter housing, that is disposed just before the outlet zone and that provides the support for the plurality of filter elements. The plurality of filter elements are disposed in an array and includes bridging members or elements that connect between adjacent filter elements, and that forms with the filter elements, a closed interstitial space between adjacent filter elements for liquid flow; a nanoscale barrier on interior surfaces, including that of the regenerative media itself, providing a mechanism to disrupt the cell wall of microscopic viruses and organisms.
Owner:BLUE SKIES GLOBAL LLC

Regenerative media filtration

A regenerative filter that includes a filter housing having inlet and outlet zones; a fluid path provided between the inlet and outlet zones; a plurality of filter elements each having an outer surface filter media applied thereto and functioning to filter particulate or contaminants from the fluid path; and a tube sheet that is supported across the filter housing, that is disposed just before the outlet zone and that provides the support for the plurality of filter elements. The plurality of filter elements are disposed in an array and includes bridging members or elements that connect between adjacent filter elements, and that forms with the filter elements, a closed interstitial space between adjacent filter elements for liquid flow; a nanoscale barrier on interior surfaces, including that of the regenerative media itself, providing a mechanism to disrupt the cell wall of microscopic viruses and organisms.
Owner:BLUE SKIES GLOBAL LLC

Method for extracting astaxanthin by using biological enzyme

The invention discloses a method for extracting astaxanthin by using a biological enzyme, which comprises the following steps of: decomposing cell walls of haematococcus pluvialis at a certain temperature by using the biological enzyme, releasing astaxanthin from the haematococcus pluvialis, dissolving out the astaxanthin by using a certain solvent, and separating and purifying to obtain powdery astaxanthin. According to the method disclosed by the invention, wall breaking is carried out by combining ultrasound and biological enzyme, and a mixed solvent of ethanol and Tween 80 is adopted, so that the extraction rate of astaxanthin reaches 98.1%, the wall breaking time is shortened, and the efficiency is improved.
Owner:ERFA BIOTECHNOLOGY (JIAXING) CO LTD

Algicidal bacterium and application thereof

The invention discloses an algicidal bacterium and application thereof, the algicidal bacterium is named as Bacillus sp.L1.3, and is preserved in China Center for Type Culture Collection on March 31, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 2025629. The nucleotide sequence of the 16S rRNA gene of the strain is as shown in SEQ ID NO: 1. The invention also provides application of the algicidal bacteria in treatment of water bloom of freshwater cyanobacteria, and the freshwater cyanobacteria is microcystis aeruginosa. Fermentation liquor of the strain L1.3 has a strong inhibition effect on growth of microcystis aeruginosa, secretions of the strain L1.3 can destroy cell walls, cell membranes, thylakoid and other structures of algae cells, inhibit photosynthesis of algae and cause irreversible damage to the algae, and the strain L1.3 has wide-range temperature stability and can be used for preparing the microcystis aeruginosa strain L1.3. The bacterial strain is suitable for being prepared into an algicidal bacterial agent to be applied to the field of biological control of freshwater cyanobacterial bloom.
Owner:FUYANG NORMAL UNIVERSITY

Curcumin-loaded MMP response type melittin nano-pellicle vesicle as well as preparation method and application of curcumin-loaded MMP response type melittin nano-pellicle vesicle

PendingCN121868251ABacteriaAntibody mimetics/scaffoldsCalcium phosphate coatingCell membrane
The invention relates to a curcumin-loaded MMP response type melittin nano-pellicle vesicle as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations. The preparation method comprises the following steps: firstly, preparing curcumin entrapped lipidosome; then carrying out culture amplification on engineering bacteria carrying melittin recombinant plasmids, extracting cell membranes after removing cell walls, and carrying out ultrasonic treatment and membrane extrusion to obtain melittin cell membrane nano-vesicles; fusing the curcumin lipidosome and the cell membrane nano-vesicles in an ultrasonic extrusion mode to obtain fused vesicles; and finally, carrying out surface calcium phosphate mineralization treatment on the fused vesicles to obtain the curcumin-loaded MMP response type melittin nano pellicle vesicles. The nano mycofilm vesicle prepared by the invention can be used for preparing antitumor drugs, and the biocompatibility and in-vivo stability of nanoparticles are improved by introducing a calcium phosphate coating; through combined delivery of melittin and curcumin, the anti-tumor effect is enhanced, so that the growth and proliferation of tumor cells are inhibited.
Owner:DALIAN UNIV OF TECH

Negative Poisson's ratio cell with embedded symmetrical broken subcell structure

The invention relates to the technical field of mechanical metamaterials, in particular to a negative Poisson's ratio cell element with an embedded symmetrical broken subcell structure, which comprises a concave hexagonal main body, the concave hexagonal main body comprises two horizontal cell walls and inclined cell walls arranged at two ends of the two horizontal cell walls, and the central points of the two inclined cell walls are bent towards one side close to the central points of the horizontal cell walls; the number of the bent wall rods is two, and the two bent wall rods are symmetrically arranged in the inwards-concave hexagonal body. The inherent contradiction that the stretching performance must be sacrificed when the rigidity of a traditional concave honeycomb structure is improved is successfully solved, and the negative Poisson's ratio effect and the energy absorption capacity of the structure can be further improved while the structural rigidity is enhanced.
Owner:NANJING TECH UNIV

Process for synergistically extracting rape pollen polysaccharide through compound enzyme

The invention relates to the technical field of plant polysaccharide extraction, in particular to a process for synergistically extracting rape pollen polysaccharide by a compound enzyme, which comprises the following steps: crushing rape pollen to 200-300 meshes by pulse airflow, performing microwave-ultrasonic degreasing by using a 85% ethanol solution, mixing with an acetic acid-sodium acetate buffer solution with the pH value of 4.8, and performing ultrasonic extraction to obtain a crude extract; the method comprises the following steps: adding a compound enzyme system which is immobilized by Fe3O4 (at) SiO2 nanoparticles by cellulase, pectinase, protease and beta-glucosidase according to a mass ratio of 2: 1: 1: 0.5, carrying out enzymolysis for 4 hours in a 50 DEG C constant-temperature water bath and a 15V / cm pulsed electric field, carrying out enzyme deactivation on enzymatic hydrolysate, carrying out gradient ethanol precipitation, carrying out ultrafiltration deproteinization, and carrying out vacuum pulsation freeze drying to obtain the polysaccharide. According to the method disclosed by the invention, the cell wall breakage rate and the enzymolysis efficiency are improved through pulsed airflow crushing, magnetic nano immobilized enzyme and pulsed electric field assistance, the polysaccharide yield reaches 7.32%, the purity is 91%, the beta-glucosidic bond proportion and the antioxidant activity are remarkably improved, the enzyme can be recycled and reused, the drying time is shortened, and efficient and low-consumption extraction is realized.
Owner:JINGMEN FARMAX AGRI TECH CO LTD

A reverse osmosis membrane cleaning agent composed of a non-oxidizing bactericide and an enzyme and its application.

This invention relates to the field of water treatment technology, specifically to a reverse osmosis membrane cleaning agent composed of a non-oxidizing bactericide and an enzyme, and its application. Its main purpose is to remove biofouling from reverse osmosis membranes and restore membrane flux. This patent discloses a novel reverse osmosis membrane cleaning agent formulation. Considering the different pH requirements of the cleaning agent in actual production, different types of bioactive hydrolytic enzymes are selected to disrupt the structure of bacterial cell walls and their extracellular polymers. A reducing agent and a non-oxidizing bactericide are combined to enhance the cleaning effect of the reverse osmosis membrane. For different cleaning purposes, non-oxidizing bactericides suitable for acidic and alkaline cleaning are formulated. Sodium benzoate and benzalkonium chloride show good application prospects in the removal of reverse osmosis membrane fouling.
Owner:ZHEJIANG UNIV

Sludge treatment method and system

The invention discloses a sludge treatment method and system, and relates to the field of solid waste treatment, and the method specifically comprises the following operation steps: S1, multi-frequency resonance pretreatment: destroying a sludge colloid structure through stepped mechanical vibration: collapsing an extracellular polymer network at a low frequency, breaking down microbial cell walls at an intermediate frequency to release intracellular water, pathogenic bacteria are inactivated through a high-frequency cavitation effect; s2, performing electrochemical in-situ flocculation; s3, gradient pressure dehydration; s4, low-temperature mineralization stabilization; and S5, vacuum condensation and recovery. According to the sludge treatment method and system, through stepped low-frequency, medium-frequency and high-frequency mechanical resonance, the colloid structure of the sludge can be directionally damaged, the fluidity of the sludge is remarkably improved, chemical conditioners are remarkably reduced, agent residues and secondary pollution are avoided, pathogens are physically inactivated through the high-frequency cavitation effect, and the treatment effect of the sludge is improved. Micro-nano oxygen bubbles are synchronously generated to strengthen free radical oxidation, and a sterile environment is created for subsequent mineralization.
Owner:广东沁桦智能环境技术有限公司

Methanogen lyase homologous with PeiR lyase sequence and application of methanogen lyase

The invention discloses methanogen lyase homologous with a PeiR lyase sequence and application of the methanogen lyase, and aims to solve the problem that PeiR lyase protein of a methanogen cell wall peptide bond can participate in a biological process of hydrolyzing archaea cell walls, effectively kill methanogen and reduce methane production, and protein homologous with the PeiR lyase protein has potential methane reduction potential. According to the invention, a series of biological information software is utilized to identify protein sequences coded by methanogens virus from rumen microbiome sequencing data, and sequence homology between the proteins and PeiR protein is further analyzed to obtain a series of methanogens lyases. In order to realize the expression of the lyase, multiple segments of primers are designed to synthesize a target sequence, and the target sequence is successfully expressed in a prokaryotic expression system. Through the detection of crude enzyme liquid, the effectiveness of the lyase in the aspect of reducing the methane yield is verified in an in-vitro gas production test.
Owner:ZHEJIANG UNIV

Nickel-free hole sealing agent and preparation method thereof

The invention relates to a nickel-free hole sealing agent and a preparation method thereof, and belongs to the technical field of high polymer materials. The nickel-free hole sealing agent comprises the following components in parts by weight: 3-10 parts of cobalt acetate, 1-11 parts of a corrosion-resistant modifier, 1-6 parts of ethylenediamine tetraacetic acid, 3-7 parts of sodium silicate and 5-15 parts of deionized water, an intermediate product containing bis-imidazoline in the corrosion-resistant modifier is tightly adsorbed on the metal surface by virtue of electrostatic attraction and a large pi bond to form a tough protective film, so that a water-phase corrosive medium is effectively isolated, the corrosion speed is remarkably reduced, and meanwhile, the corrosion-resistant modifier has good heat-resistant stability and still has a corrosion inhibition effect in a high-temperature environment; the glycine enables the nickel-free hole sealing agent to better fill tiny holes in the metal surface, so that the surface is flat and smooth, and uniform distribution and permeation of the hole sealing agent are promoted; quaternary ammonium salt destroys cell walls and cell membranes of microorganisms and inhibits growth and reproduction of the microorganisms; the nickel-free hole sealing agent prepared by the invention not only has a good antistatic effect, but also has excellent high-temperature-resistant and antibacterial effects.
Owner:DONGGUAN WEIYUAN TECH CO LTD

Cooking method of pressure cooker

The invention discloses a cooking method of a pressure cooker, which comprises the following steps: a boosting cooking stage: starting a heating device to enable the cooking pressure of the pressure cooker to reach a first preset cooking condition; in the variable-pressure wall breaking stage, a heating device is turned off, a cooling device is turned on to enable the cooking pressure of the pressure cooker to be rapidly reduced from the first preset cooking condition to a second preset cooking condition, so that cell walls of cooking food materials are broken under the action of pressure difference formed in a short time, and the second preset cooking condition at least comprises that the cooking pressure is larger than normal pressure. When the cooking pressure is rapidly reduced from the first preset cooking condition to a second preset cooking condition, the rapid pressure reduction rate is at least 1.8 times of the natural pressure reduction rate. Through the method, food materials which are not easy to cook and have thick cell walls can be cooked in a short time, meanwhile, nutrient substances are fully dissolved out, and the cooking taste is improved.
Owner:JOYOUNG CO LTD

Compositions and methods to improve sequestration of mycotoxins and their use in detoxification of animal feed and animal production systems

PendingUS20260053874A1AntimycoticsProtozoa material medical ingredientsCell wallMycotoxin contamination
The present disclosure relates to compositions and methods comprising a yeast cell wall extract and a clay material admixed to a proteinaceous and / or cell wall extract(s) from bacterial or fungal fermentation from amino acid production. In another embodiment, the present compositions comprise a yeast cell wall extract, a clay material, and an algal material admixed to a proteinaceous and / or cell wall extract(s) from bacterial or fungal fermentation from amino acid production. This disclosure also relates to methods of utilizing the same to sequester and / or adsorb mycotoxins internal or external to an animal, thereby reducing and / or preventing the toxic and harmful effects of mycotoxin contamination and / or infection in animals. Specifically, application of the present methods and compositions are found in the dietary administration and / or co-administration of the present compositions to animals in animal feed and / or animal production systems, as well as in therapeutic, prophylactic, and research applications.
Owner:ALLTECH CO LTD

Preparation method of saccharomyces cerevisiae-sourced yeast beta-glucan

The invention belongs to the technical field of saccharomyces cerevisiae beta-glucan, and relates to a preparation method of saccharomyces cerevisiae-sourced yeast beta-glucan. The invention relates to a preparation method of saccharomyces cerevisiae-sourced yeast beta-glucan, which comprises the following steps: uniformly mixing a yeast raw material with a first solvent, and carrying out high-pressure homogenization to obtain a homogenized feed liquid; adding food-grade alkali into the homogenized feed liquid to carry out extraction reaction, centrifuging the obtained reaction extracting solution, and separating a heavy phase to obtain a heavy-phase insoluble cell wall extract; and continuously adding a second solvent for resuspension, adding a compound enzyme preparation, adjusting the pH value of the reaction system by using an acidic reagent, carrying out an enzymatic hydrolysis reaction, centrifuging after the reaction is finished, recovering a heavy phase, and drying to obtain the insoluble yeast beta-glucan dry powder. According to the preparation method of the yeast glucan, provided by the invention, the conventional preparation process flow is shortened, the environmental protection treatment pressure is greatly relieved, the production cost is effectively reduced, and the prepared yeast glucan is higher in purity and has a better industrial application prospect.
Owner:NANJING BIOTOGETHER

Method for synergistically preparing recycled aggregate from urban updated garden waste and building waste

The invention discloses a method for synergistically preparing recycled aggregate from urban renewal garden waste and building waste, and relates to the field of treatment of garden waste and building waste. The method comprises the following steps: carrying out molecular-level deconstruction pretreatment on garden wastes, destroying cell walls by adopting dual-frequency ultrasound combined with biological enzymolysis, and treating the building wastes by combining high-pressure water jet with abrasive impact; performing nano-scale composite granulation, adding a nano-composite binder, and introducing CO2 to form a gel transition layer; by means of plasma-assisted sintering, particle diffusion is accelerated by means of radio-frequency plasma and an electric field; chemical vapor deposition is adopted, and a TiN-TiO2 gradient functional coating is formed; real-time monitoring and regulation are performed through a multi-mode sensor network and an intelligent algorithm, and process parameters are adjusted according to a product performance prediction result. The recycled aggregate disclosed by the invention is high in strength, good in heat preservation and strong in freezing resistance; negative carbon emission reduction is achieved in the production process, an intelligent system guarantees stable product quality, environmental protection and economic benefits are achieved, and urban update and solid waste treatment green transformation are promoted.
Owner:安徽格林生态环境科技有限公司

Siberian elm root extract as well as extraction method and application thereof

According to the elm root extract and the extraction method and application thereof, ultrasonic-assisted staged enzymolysis is adopted, elm roots are decomposed through the special activity of various enzymes, cells are broken, and the material transfer barrier between cell walls and cells is reduced, so that the purpose of extracting effective active ingredients of polyphenol and polysaccharide in the elm roots to the maximum extent is achieved. According to the invention, the effective active ingredients in the elm root extract are increased in a targeted manner, and it is proved that the elm root extract has an anti-inflammatory effect and also has an anti-skin aging cosmetic application, especially a tightening effect, and has a good application prospect in cosmetic compositions. The composition can be applied to personal care products with anti-aging and firming effects.
Owner:MAKEUP (GUANGZHOU) BIOCHEMICAL TECH CO LTD

Method for extracting PN / PDRN from lactobacillus bulgaricus and application of PN / PDRN in relieving and anti-inflammatory aspects

The invention discloses a method for extracting PN / PDRN from lactobacillus bulgaricus and application of the PN / PDRN in relieving and anti-inflammatory aspects, belongs to the technical field of biochemistry, and solves the problems that in the prior art, the acquisition source of PN / PDRN is unstable, and pollution easily exists. According to the preparation method, cell walls of lactobacillus bulgaricus are preliminarily dissolved through alkaline conditions and lysozyme in the preparation process, cell membranes of bacteria are destroyed through lauryl sodium sulfate to be split, the conditions are mild, meanwhile, PVP is added to protect a PN structure, and the purity of the PN / PDRN is effectively improved through a final product prepared through the preparation method.
Owner:SHAANXI MICROBIOLOGICAL TECH CO LTD

Methanogen lyase homologous with high-dimensional characteristics of PeiW lyase and application of methanogen lyase

The invention discloses methanogen lyase homologous with high-dimensional characteristics of PeiW lyase and application of the methanogen lyase, and aims to solve the problem that PeiW lyase protein of methanogen cell wall peptide bonds can participate in the biological process of hydrolyzing archaea cell walls, effectively kill methanogen and reduce methane production, and protein homologous with the protein has potential methane reduction potential. According to the method, a series of bioinformatics software is utilized, a protein sequence coded by methanogens virus is identified from a large amount of rumen microorganism virus group sequencing data, the high-dimensional homology of a deep learning model between the protein sequence and PeiW is analyzed, and two methanogens lyases similar to the high-dimensional characteristics obtained by the PeiW model are obtained. In order to realize the expression of the lyase, multiple segments of primers are designed to synthesize a target sequence, and the target sequence is successfully expressed in a prokaryotic expression system. Through detection of a crude enzyme solution, the effectiveness of the lyase in the aspect of reducing the yield of methane is verified in an in-vitro gas production test.
Owner:ZHEJIANG UNIV

Hair-blacking composition for promoting melanin secretion and preparation method thereof

The invention relates to the technical field of scalp care, in particular to a hair-blacking composition for promoting melanin secretion and a preparation method of the hair-blacking composition. Comprising the following components in parts by weight: water, ethanol, a ginseng root extract, a polygonum multiflorum extract, a cacumen biotae extract, a licorice root extract, an eclipta prostrata extract, a ganoderma atrum extract, a rhizoma bletillae root extract, a mulberry leaf extract, radix sophorae flavescentis powder, a glossy privet fruit extract, a raspberry fruit extract and a black sesame extract. According to the technical scheme, the ganoderma atrum extract is prepared through enzymolysis-ultrasonic combined modification, a cell wall structure is decomposed through compound enzyme, then cell remains are further broken through the ultrasonic cavitation effect, and the release rate of active peptide and polysaccharide is increased; the hair-blacking composition can promote the generation of hair melanin so as to blacken hair, improve the hair quality, enable the hair to become tough and elastic, and enable the hair to gradually become black, smooth and firm after being smeared on the scalp for a long time.
Owner:SHENZHEN XINYUANYUAN PLANT TECH DEV CO LTD

Star-shaped negative Poisson's ratio honeycomb structure with double stress platforms

A star-shaped negative Poisson's ratio honeycomb structure with double stress platforms comprises a plurality of star-shaped cell elements which are periodically arranged in the same plane in the transverse direction and the longitudinal direction, and each star-shaped cell element comprises a quadrangular star-shaped structure, a circular structure and two double-arrow-shaped structures; the quadrangular star-shaped structure is formed by connecting eight inclined cell walls end to end, so that a closed hollow structure which is symmetrical in the up-and-down and left-and-right directions is formed; the circular structure is embedded in the quadrangular star-shaped structure and intersects with four concave points of the quadrangular star-shaped structure; the two double-arrow-shaped structures are located on the left side and the right side of the quadrangular star-shaped structure respectively, each double-arrow-shaped structure comprises two long cell walls and two short cell walls, and the intersection point of the two long cell walls coincides with the inward concave point of the quadrangular star-shaped structure. Under the action of longitudinal compression, the double-stress-platform characteristic is shown, the good negative Poisson's ratio effect is achieved, the structural bearing capacity and the energy absorption efficiency can be improved, and the double-stress-platform metamaterial belongs to the technical field of mechanical metamaterials.
Owner:SOUTH CHINA UNIV OF TECH

Methanogen lyase having same protein family annotation as PeiR lyase and use thereof

The invention discloses methanogen lyase with the same protein family annotation as PeiR lyase and application of the methanogen lyase, and the methanogen lyase is obtained through homologous screening of Pfam protein family database annotation based on known PeiR lyase characteristics aiming at methanogen cell wall peptide bonds. The PeiR lyase protein can participate in the biological process of hydrolyzing archaea cell walls, methanogens are effectively killed, methane production is reduced, and the protein homologous with the protein has potential methane reduction potential. According to the invention, by integrating metagenome sequencing data of rumen microorganisms, screening methanogenic bacterium virus protein by using series bioinformatics software, and combining Pfam functional domain annotation to carry out homologous relationship analysis, a series of lyase is finally identified and is successfully expressed in a prokaryotic expression system, and meanwhile, an in-vitro gas production experiment result also shows that the methanogenic bacterium virus protein can be successfully expressed in a prokaryotic expression system. The crude enzyme liquid can significantly reduce the methane generation amount.
Owner:ZHEJIANG UNIV

Methanogen lyase homologous with high-dimensional characteristics of PeiR lyase and application of methanogen lyase

The invention discloses methanogen lyase homologous with high-dimensional characteristics of PeiR lyase and application of the methanogen lyase and aims to solve the problems that PeiR lyase protein of methanogen cell wall peptide bonds can participate in the biological process of hydrolyzing archaea cell walls, methanogen is effectively killed, methane production is reduced, and protein homologous with the protein has potential methane reduction potential. According to the invention, a series of biological information software is utilized to identify protein sequences coded by methanogen viruses from a large amount of rumen microbiome sequencing data, and high-dimensional homology between the proteins and PeiR proteins based on a deep learning model TM-Vec is further analyzed, so that a series of methanogen lyases are obtained. In order to realize the expression of the lyase, multiple segments of primers are designed to synthesize a target sequence, and the target sequence is successfully expressed in a prokaryotic expression system. Through the detection of crude enzyme liquid, the effectiveness of the lyase in the aspect of reducing the methane yield is verified in an in-vitro gas production test.
Owner:ZHEJIANG UNIV

A method for extracting astragalus polysaccharide

This application relates to the technical field of astragalus polysaccharides, and specifically relates to a method for extracting astragalus polysaccharides. A method for extracting astragalus polysaccharides includes the following steps: primary water extraction, secondary water extraction, vacuum concentration, primary alcohol precipitation, secondary alcohol precipitation, and purification. Among them, in the primary water extraction: soak astragalus with a weak alkaline solution, then heat to boiling and carry out extraction, and filter to separate the primary precipitate and the primary extract. By using a weak alkaline solution in the primary water extraction step, this application destroys cell walls, increases the solubility of polysaccharides, and promotes the release of polysaccharides, significantly improving the extraction rate of astragalus polysaccharides.
Owner:NINGBO DACHANG PHARMA

Wheat stem rot regulatory gene Tatrx-m, encoding protein thereof, recombinant vector and application of wheat stem rot regulatory gene Tatrx-m

The invention relates to a wheat stem rot regulatory gene Tatrx-m, and an encoding protein, a recombinant vector and an application of the wheat stem rot regulatory gene Tatrx-m. The genomic sequence of the gene is SEQ ID NO.1, the CDS sequence of the gene is SEQ ID NO.2, and thioredoxin shown as SEQ ID NO.3 is encoded. The invention further provides a specific primer pair used for gene identification, a silent vector (Tatrx-m-VIGS) and an overexpression vector (LGY-OE3-Tatrx-m) are constructed, and the function of the primer pair is verified through a virus-induced gene silencing (VIGS) technology and agrobacterium-mediated transformation of wheat. Experiments show that silence of Tatrx-m results in significant reduction of wheat stem rot resistance, and overexpression of the gene can improve resistance. Based on hypha quantification, DAB staining, H2O2 content and cell death analysis, Tatrx-m is revealed to enhance wheat resistance by regulating active oxygen removal and cell wall strengthening pathways. The gene TaTrx-m has the positive regulation effect in wheat stem rot for the first time, can be applied to disease-resistant molecular marker development, gene editing and transgenic breeding, provides core genetic resources for disease-resistant variety breeding, and has theoretical and application values.
Owner:HENAN AGRICULTURAL UNIVERSITY

Vertical differential pressure sterilizer and sterilization method

The invention discloses a vertical differential pressure sterilizer and a sterilization method thereof, and belongs to the technical field of medical instrument sterilization equipment. The invention aims to solve the problems of long sterilization period and possible damage to effective components of materials in the existing sterilization technology. According to the technical scheme, the vertical differential pressure sterilizer comprises a vertical three-layer barrel (an inner barrel, a steam interlayer and a heat preservation layer), a vacuum pump set, a straight-through steam control valve set, a jacket steam control valve set and a jacket cooling control valve set. The inner cylinder is firstly vacuumized, then the steam interlayer and the straight steam are used for heating materials together, and the steps of vacuumizing and steam filling are repeated for multiple times, so that cell wall structures of microorganisms are damaged under the alternate action of vacuum and pressure, and rapid sterilization is realized at a relatively low temperature. And after sterilization, cooling water is injected into the steam interlayer to realize rapid cooling. The device has the advantages of being compact in structure, rapid in sterilization, energy-saving, environment-friendly and capable of effectively protecting material components.
Owner:JIANGXI HEBO KANGHUA PHARM EQUIP CO LTD

Sludge treatment device based on flash evaporation

The utility model relates to a sludge treatment device based on flash evaporation, which comprises a primary flash evaporation system and any one of a hydrolysis tank and a secondary flash evaporation system. The sludge is subjected to resourceful treatment in a flash evaporation and pyrohydrolysis and / or two-stage flash evaporation mode, cell walls in the sludge can be fully and effectively broken under the low-temperature condition through the flash evaporation technology, moisture and solute in cells are released, the dehydration performance of the sludge is improved, and the dehydration efficiency of the sludge is improved. The wall-broken sludge is conveyed to the hydrolysis tank for hydrolysis and / or the second flash evaporation tank for flash evaporation and drying, and the wall-broken sludge is more easily hydrolyzed and / or dried. According to the sludge drying device, the problems of high energy consumption, large heat loss and long treatment time in the sludge drying process are solved, and the productivity is greatly improved.
Owner:NANJING TECH UNIV

Special silicon-containing compound fertilizer for lodging resistance and disease prevention of rice and preparation method of special silicon-containing compound fertilizer

The invention relates to the technical field of compound fertilizers, and discloses a silicon-containing compound fertilizer special for lodging resistance and disease prevention of rice and a preparation method thereof.The silicon-containing compound fertilizer is prepared from 30-35 parts of organic compound matrix, 20-26 parts of straw liquefied matter, 5-10 parts of water-soluble silicon fertilizer, 4-8 parts of humic acid, 1-1.5 parts of microbial agent, 0.5-0.8 part of polyglutamic acid, 5-10 parts of compound coating liquid and 0.2-0.5 part of microelement chelate, the composted duck manure, the peat and the coco coir are used as main raw materials, the air permeability of the peat is combined with nutrients of the duck manure, soil hardening can be improved, soil fertility can be improved, the water-soluble silicon fertilizer is matched with the organic compound matrix, absorption of silicon elements by rice is promoted, a silicified cell layer is formed on the stem epidermis, then the cell wall thickness is increased, and the rice yield is increased. The breaking strength of the stalks is improved.
Owner:HUBEI EZHONG ECOLOGICAL ENG CO LTD

Aqueous polyhydroxyalkanoate coating, preparation method therefor and use thereof

The present invention provides an aqueous polyhydroxyalkanoate coating, a preparation method therefor, and a use thereof. The preparation method for the aqueous polyhydroxyalkanoate coating comprises: subjecting a polyhydroxyalkanoate fermentation solution to cell wall breaking and extraction, to obtain an aqueous polyhydroxyalkanoate dispersion, and then mixing the aqueous polyhydroxyalkanoate dispersion with an additive to obtain the aqueous polyhydroxyalkanoate coating, a dispersant and talcum powder being added during the cell disruption process. By means of using talcum powder to replace a portion of a dispersant as a protective additive for PHA particles, the present invention can prevent the aggregation of PHA particles at high purity, and the resulting aqueous PHA coating exhibits good coating water resistance.
Owner:BEIJING PHABUILDER BIOTECHNOLOGY CO LTD

Preparation method of yeast beta-glucan

PendingCN120309757ABiotechnologyFreeze-drying
The invention discloses a yeast beta-glucan preparation method based on high pressure-microjet homogenization treatment, and belongs to the technical field of zymosan extraction. The method comprises the following steps: carrying out high pressure-microjet homogenization pretreatment on active dry yeast, then carrying out autolysis treatment, then extracting beta-glucan by an acid-alkali method, and finally carrying out freeze drying to obtain a high-purity product. The core of the method is that the yeast cell walls are subjected to physical wall breaking by adopting a high pressure-microjet homogenization technology, and the extraction rate and the purity of the beta-glucan are remarkably improved by combining with an optimized acid-alkali treatment process. Compared with a traditional enzymolysis method, the method has the advantages that damage of exogenous enzyme to the beta-glucan structure is avoided, the purity of the obtained product reaches 84% or above, the content of protein impurities is lower than 0.2%, a new way is provided for efficient utilization of yeast cell wall resources, and the method is suitable for the fields of food, medicine and health care products.
Owner:HUAZHONG AGRI UNIV