101 results about "Cell bodies" patented technology

The invention provides a wide-temperature-zone in-situ pool body suitable for an XAFS transmission mode. The wide-temperature-zone in-situ pool body comprises a bottom plate, vertical plates are symmetrically and fixedly connected to the top face of the bottom plate, an auxiliary detection assembly is installed between the two vertical plates, an opening and closing assembly is installed on one sides of the two vertical plates, and one end of the opening and closing assembly is connected with the auxiliary detection assembly; the auxiliary detection assembly comprises a protection box, the protection box is fixedly connected between the two sets of vertical plates, round holes are formed in the two sides of the protection box respectively, an air inlet connector is fixedly connected to the bottom face of the protection box, and an exhaust connector is fixedly connected to one side of the protection box; inner cavities of the air inlet connector and the air outlet connector are communicated with an inner cavity of the protection box, and the two ends of the top face of the protection box are fixedly connected with a water inlet connector and a water drainage connector respectively. Through mutual cooperation of the auxiliary detection assembly, the opening and closing assembly and the feeding and discharging assembly, automatic feeding and discharging can be achieved, and fed materials can be detected.

The invention belongs to the technical field of porous medium combustors, and particularly relates to a model selection construction method of a periodic cell body structure of a porous medium combustor, which comprises the steps of constructing a heat transfer device, constructing a cell body unit to be selected, installing the cell body unit to be selected, measuring data parameters, analyzing results and the like. From a plurality of perspectives of convective heat transfer coefficient, area excellent factor, effective heat conductivity coefficient, longitudinal diffusion coefficient and the like, the performance of the porous medium burner can be influenced by using different periodic cell body structures under the condition of the same porosity; the invention further provides a model selection construction method for the periodic cell body structure when the porosity is the same, a theoretical basis is provided for model selection of the periodic cell body structure of the porous medium burner, and several periodic cell body structures recommended to be constructed and used under common porosity conditions are given.

The invention relates to a method and a system for simultaneously acquiring complete form and omics molecular information of a single neuron. The method comprises the following steps: acquiring fluorescently-labeled samples of cell bodies, dendrites and axons of the neuron; performing cyclic operation of scanning imaging and cutting on the section of the sample until the scanning imaging of the whole sample is completed, and collecting a section containing a fluorescence-labeled neuron cell body obtained by cutting; the method comprises the following steps: reconstructing a sample section three-dimensional image obtained based on scanning imaging operation to obtain a complete form of neurons, extracting fluorescently-labeled neuronal cell bodies from collected slices, and obtaining omics molecular information of the neurons through omics analysis. According to the method, the fine and complete form and omics molecular information of the same neuron can be effectively integrated, and the problem that the two kinds of key information cannot be obtained at the same time at the single neuron precision level is successfully solved.

The invention relates to the technical field of biology, in particular to a human cholangiocarcinoma drug-resistant cell line HuCCT1-GEM-2 and application thereof.The human cholangiocarcinoma drug-resistant cell line HuCCT1-GEM-2 is preserved in the China Center for Type Culture Collection on May 11, 2023, the preservation number is CCTCC NO: C2023103, the IC50 value of the human cholangiocarcinoma drug-resistant cell line HuCCT1-GEM-2 to gemcitabine is 314.5 mu M, the IC50 value of a parent cell HUCCT1 to gemcitabine is 12.7 mu M, the drug-resistant index is up to 24.8, and the human cholangiocarcinoma drug-resistant cell line HuCCT1-GEM-2 can be used as a drug-resistant cell line. The cell body is obviously enlarged and rounded or is more irregular in shape, the specific volume of the cell cytoplasm is larger, binuclear or multinuclear appears in the cell, and the drug-resistant cell presents obvious bulk aggregation growth; after 24 hours, the growth rate of the HuCCT-1-GEM-2 cell is obviously slower than that of a parent cell, and the HuCCT-1-GEM-2 cell can be used for researching the drug resistance mechanism of bile duct cancer.

The invention provides an electrolytic bath system. The electrolytic bath system comprises an electrolytic bath unit and a gas supply assembly. Each electrolytic cell unit comprises a cell body and a polar plate and a diaphragm which are arranged in the cell body, the diaphragm is used for dividing the cell body into an anode chamber and a cathode chamber, the positive electrode side of the polar plate is located in the anode chamber, the negative electrode side of the polar plate is located in the cathode chamber, and the electrolytic cell units are sequentially connected in series. And the gas supply assembly communicates with the lower portions of the anode chambers of all the electrolytic cell units, the gas supply assembly is used for supplying diluent gas into all the anode chambers when the amount of oxygen generated in the anode chambers is lower than a first preset threshold value, and the diluent gas is used for reducing the hydrogen content of gas in the anode chambers. According to the electrolytic bath system, the diluent gas can be supplied into the anode chamber through the gas supply assembly, so that the hydrogen content of the gas in the anode chamber is reduced, and the purpose of safe operation in a low-load state is achieved.

The present invention discloses an in-situ infrared electrochemical cell for analysis and detection, which is used to be placed inside an infrared spectrometer and connected to the infrared spectrometer for use. The in-situ infrared electrochemical cell includes a cell body for containing electrolyte and a base for fixing the cell body; the cell body includes a left cell body, a right cell body, and a connection portion connecting the left cell body and the right cell body; a counter electrode is provided on the top of the cell body, and a reference electrode is provided on the top of the right cell body; the base is located directly below the right cell body, and a receiving space with an upward opening is provided inside the cell body, which can be used to partially accommodate the reaction chamber of the right cell body, and a working electrode is provided on the side of the base. Through the above-mentioned method, an in-situ infrared electrochemical cell is provided that can achieve electrolyte convection balance, low electrolyte consumption, high detection sensitivity, and simple and quick assembly and cleaning of the electrochemical cell. The in-situ infrared electrochemical cell is used for analysis and detection to achieve ultra-sensitive quantitative detection of metal ions.

A neurite growth stimulant is a small molecule ENPP1 inhibitor. Small molecule ENPP1 inhibitors are capable of treating central nervous system (CNS) injury and promoting axonal regeneration. The ENPP1 inhibitor in the carrier can be administered at damaged nerves and their cell bodies to promote the regeneration of axons.

Methods of treating epilepsy are provided. Accordingly, there is provided a method of treating epilepsy in a subject in need thereof, the method comprising: (a) administering into a thalamus nucleus of the subject a therapeutically effective amount of a polynucleotide encoding a bistable type II opsin attached to a heterologous ER export signal and / or membrane trafficking signal which enables trafficking of said bistable type II opsin to axonal presynaptic terminals; and (b) exposing a neural region of said subject to light in a wavelength that activates said bistable type II opsin, wherein said neural region comprises a cell body and / or an axon of said thalamus nucleus.

Provided are a universal cell system, a construction method therefor, and use thereof. Universal mesenchymal stem cells are constructed by means of the combination of electroporation and CRISPRoff gene editing, and the cells can survive stably in a pulmonary inflammatory pathological environment to provide sustained treatment, significantly improve multiple indicators of acute pneumonia, solve immune rejection, and provide new cell therapies and ideas for acute pneumonia. CRISPRoff is introduced into universal CTL cells by means of electroporation technology, targeting a specific region and inhibiting the expression of HLA-I in T cells; thus, T cells with low immunogenicity are constructed, and the cells can avoid allogeneic immune rejection. The universal cell system combines the advantages of both transient transfection and CRISPRoff gene editing, has great significance in the field of cell therapy, provides a more comprehensive and effective solution for the treatment of various diseases, and has a wide application prospect.

To provide cell bodies to be used for medical treatment.SOLUTION: Cell bodies 9 to be used for medical treatment are prepared in medical treatment by a method including the steps of: preparing a sample holder including a holding space for holding a fluid medium 11; preparing a sample 7 including the one or more cell bodies in fluid medium in the holding space; preparing a functionalized wall surface portion 17 to be in contact with the sample in the holding space; bringing the sample into contact with the functionalized wall surface portion; pushing the one or more cell bodies of the sample in the holding space in a direction separating away from the functionalized wall surface portion; and removing at least some of the cell bodies of the sample from the holding space after the at least some of the cell bodies are separated away from the functionalized surface portion, where the removed cell bodies are used to be administered to a subject or another subject for medical treatment.SELECTED DRAWING: Figure 2A

The application discloses an engineered umbilical cord stem cell and application thereof in preparation of anti-aging drugs and medical beauty products. The application constructs an engineered cell system with high anti-aging function by designing a fusion polypeptide TAT-SIRT1-SOD (SEQ ID NO: 1) and an anti-p16INK4a monoclonal antibody (mAb-p16) to cooperatively modify the umbilical cord stem cell, and combining a 3D dynamic culture technology. The fusion polypeptide is mediated to cross the membrane by a TAT cell-penetrating peptide, activates SIRT1 and removes mitochondrial ROS; the mAb-p16 is specifically combined with p16INK4a on the surface of a senescent cell, and removes the senescent cell through an ADCC effect. Experiments show that the engineered umbilical cord stem cell can increase the removal rate of the senescent cell by 86.1%, increase the SOD activity by 300%, significantly inhibit the secretion of an inflammatory factor IL-6, and increase the skin collagen density by 148% and reduce the wrinkle depth by 67% in an animal model. The application provides a novel and efficient technical scheme for anti-aging drugs and medical beauty products.

The invention relates to the technical field of dendritic cell in-vitro induction methods, and provides a dendritic cell body and an in-vitro induction method and application thereof. Thymic stromal lymphopoietin; the invention relates to a recombinant human FLT3L-Fc fusion protein. And maturing stimulation combination. On the premise of avoiding complex use of equipment, the bottlenecks of DC yield, subgroup purity and functionality are broken through.

The invention provides an electrochemical in-situ reaction device for time-resolved XAFS (X-ray fluorescence spectroscopy) testing, which relates to the field of spectral analysis testing and comprises a reaction tank body, a liquid layer thickness adjusting mechanism and a visualization mechanism, the end face sealing mechanism is arranged on the side, away from the first through hole, of the second through hole and is provided with a sample mounting position; an optical window hermetically extending into the reaction tank body is formed at one end of the visualization mechanism, and the liquid layer thickness adjusting mechanism adjusts the distance between the optical window and the second through hole; when the end face sealing mechanism is hermetically connected to the reaction tank body, the sample mounting position faces the interior of the tank body. According to the invention, the distance between the optical window and the second through hole is precisely adjusted through the liquid layer thickness adjusting mechanism to precisely adjust the thickness of the liquid layer, so that the application of the millisecond-level time-resolved XAFS technology in the electrochemical liquid phase reaction direction is realized, and a high-precision in-situ reaction device is provided.

The application relates to a method and system for simultaneously obtaining complete morphology and omics molecular information of a single neuron, which comprises the following steps: obtaining a sample in which the cell body, dendrite and axon of a neuron are labeled by fluorescence; performing a cycle operation of scanning imaging and cutting on the section of the sample until the scanning imaging of the whole sample is completed, and collecting the cut section containing the fluorescence-labeled neuron cell body; based on the three-dimensional image of the sample section obtained by the scanning imaging operation, reconstructing the complete morphology of the neuron, and extracting the fluorescence-labeled neuron cell body from the collected section to obtain the omics molecular information of the neuron by omics analysis. The application can effectively integrate the fine complete morphology and omics molecular information of the same neuron, and successfully solves the problem that the two key information cannot be simultaneously obtained at the single neuron precision level.

A method of manipulating and / or investigating cellular bodies (9) is provided. The method comprises the steps of: providing a sample holder (3) comprising a holding space (5) for holding a fluid medium (11); providing a sample (7) comprising one or more cellular bodies (9) in a fluid medium (11) in the holding space (5); generating an acoustic wave in the holding space exerting a force (F) on the sample (7) in the holding space (5). The method further comprises providing the holding space (5) with a functionalised wall surface portion (17) to be contacted by the sample (7) and the sample (7) is in contact with the functionalised wall surface portion (17) during at least part of the step of application of the acoustic wave. A system and a sample holder (3) are also provided.

The invention relates to the technical field of biology, in particular to an in-vivo editing and metabolism reprogramming nano drug-loaded material for targeting macrophages as well as a preparation method and application of the in-vivo editing and metabolism reprogramming nano drug-loaded material. On the basis of a metal organic framework (MOF) material, CAR mRNA molecules targeting DLL3 are loaded in the MOF material, a macrophage-derived cell membrane is coated outside the MOF material, and the nano drug-loading material with the macrophage targeting capability is constructed. The material has the effects of specifically swallowing and killing tumor cells, improves the immunosuppression effect of a tumor microenvironment by utilizing the immune activation effects of succinic acid and manganese, and synergistically promotes the killing effect on small cell lung cancer. The macrophage in-vivo editing technology designed by the invention is a novel safe, efficient, simple and convenient tumor immunotherapy technology, overcomes the defect that CAR-T cannot enter the interior of a solid tumor, and solves the problem of tedious cell in-vitro amplification and gene editing procedures in the conventional CAR immunotherapy process; and a new strategy is provided for immunotherapy of small cell lung cancer with limited treatment means.

A vapor cell comprises a cell body and a cell window, which is anodically connected to the cell body. The cell window and the cell body define a vapor cavity designed to contain a vapor comprising a variety of alkali atoms.

This invention discloses a compact high-temperature, high-pressure transmission XAFS in-situ cell, comprising a cell body and a cover that are sealed together. A light-transmitting window assembly is installed on the cell body, allowing X-rays to pass through and irradiate the sample to be tested, which is installed inside the cell body. The cell body and cover are also connected to and equipped with components such as a ball valve, a gas connection, a temperature probe, test electrodes, and a heating element. The heating element is used to regulate the sample temperature, and a water-cooling structure located in the side wall of the cell body is used to regulate the cell body temperature. This invention integrates a ball valve control structure on the same cell body. When the ball valve is closed, a high-pressure atmosphere can be established and maintained inside the cell body to achieve high-temperature, high-pressure transmission XAFS experiments; when the ball valve remains open, the inside of the cell body is in a normal-pressure gas flow state, achieving high-temperature, normal-pressure transmission XAFS experiments. One device can meet the testing requirements of two different operating conditions, significantly reducing the user's equipment investment costs.

The present application relates to the technical field of kits, in particular to a kind of NK cell in vitro expansion kit, including NK cell kit body, NK cell kit body is equipped with separating plate in the middle, the two sides of separating plate are reagent bottle storage cavity and cell culture cavity respectively, the top of reagent bottle storage cavity is equipped with first isolation plate, and the top of cell culture cavity is equipped with second isolation plate.The present application is equipped with reagent bottle storage cavity and cell culture cavity in NK cell kit body, which can facilitate the culture of cells, and facilitate the storage of reagent bottles;By being equipped with jacking block in reagent bottle storage cavity, the reagent bottle in storage groove can be jacked up and ejected from the storage groove by the upward movement of jacking block driven by the downward pressure of pressure rod, so that the reagent bottle can be easily taken, and the operability is strong;By being equipped with magnetic baffle in the symmetry in universal ball, after the dropper is pulled out of the through hole, the magnetic baffle can be automatically closed under the action of magnetic force.

The utility model relates to a filtering assembly, which belongs to the technical field of air filtering equipment, and comprises at least two cell bodies, first buckles and second buckles, the two ends of the top of each cell body are provided with first clamping holes, and the two ends of the bottom of each cell body are provided with second clamping holes. The first buckle is clamped between the two opposite first clamping holes of the two adjacent cell bodies in a matched mode, the second buckle is clamped between the two opposite second clamping holes of the two adjacent cell bodies in a matched mode, and by the adoption of the structure, the multiple cell bodies are connected in a buckled mode through the first buckle and the second buckle; the buckle is arranged in the filtering section of the air filter, the buckle is limited by the inner wall of the filtering section, the buckle is prevented from being released, the connection is more reliable, the cell body can be quickly disassembled by opening the buckle when maintenance and cleaning are needed, and the working efficiency is improved.

The utility model relates to the technical field of water quality monitoring, in particular to a circulation measuring cell with a visual window, which comprises a cell body and a cell cover which are detachably connected, the cell cover is provided with an electrode extending into the cell body, the cell body is provided with the visual window, the visual window is provided with a transparent material structural member for sealing, the visual window is provided with a shielding cover, and the shielding cover is provided with a transparent material. The shielding cover is a structural part made of light-proof materials, the shielding cover is detachably connected with the visual window, and the shielding cover can shield the visual window. The visual window consisting of the visual window and the transparent material structural member is arranged on the circulation measuring cell, so that the water flow state, the electrode integrity and the invasion liquid level in the circulation measuring cell can be visually observed through the visual window, and a visual, accurate and timely monitoring result is provided for workers; meanwhile, the visual window is shielded through the shielding cover, and the situation that the normal proceeding of the water treatment process is affected by the light transmission of the visual window is avoided.

The application discloses a multi-cell horizontal distance-variable module, relates to a cell distance-variable conveying technology, and comprises a bearing base and a conveying base arranged in front of the bearing base, and a track conveying module is arranged above the conveying base and used for driving a cell body to move and convey; the top surface of the bearing base is provided with a transverse slider, and a transverse sliding frame is slidably arranged in the transverse slider; the longitudinal slider is slidably arranged in the longitudinal sliding frame, and a distance-variable mechanism is arranged at the front of the upper end of the longitudinal sliding frame; and the distance-variable mechanism comprises a clamping side frame. The multi-cell horizontal distance-variable module is driven by the transverse slider and the longitudinal slider to move, meanwhile, a plurality of distance-variable side frames are adapted to cell bodies of different specifications to adjust the distance, and a supporting mechanism above the conveying track module is used to assist the cell body to move, so that the cell body is prevented from falling and affecting the processing efficiency during distance-variable conveying operation.

The invention discloses engineered umbilical cord stem cells and application thereof in preparation of anti-aging drugs and medical beauty products. A fusion polypeptide TAT-SIRT1-SOD (SEQ ID NO: 1) and an anti-p16INK4a monoclonal antibody (mAb-p16) are designed to synergistically modify umbilical cord stem cells, and a 3D dynamic culture technology is combined to construct an engineered cell system with an efficient anti-aging function. The fusion polypeptide mediates transmembrane through TAT cell-penetrating peptide, activates SIRT1 and removes mitochondrial ROS; the mAb-p16 is specifically combined with p16INK4a on the surface of the senescent cell, and the senescent cell is eliminated through an ADCC effect. Experiments show that the engineered umbilical cord stem cell can increase the senescent cell clearance rate by 86.1%, increase the SOD activity by 300%, significantly inhibit secretion of inflammatory factors IL-6, increase the skin collagen density by 148% and reduce the wrinkle depth by 67% in an animal model. The invention provides a novel and efficient technical scheme for anti-aging medicines and medical beauty products.

Compositions comprising a condensed mesenchymal cell body and a hydrogel are provided. The compositions may further include drugs or growth factors. The condensed mesenchymal cell body may include a connective tissue cell, or even a progenitor cell capable of producing connective tissue extracellular matrices such collagen and glycosaminoglycan. Also provided are methods of treating connective tissue defects, cartilage injury, and cartilage degradation.

The invention relates to a cell housing, in particular cylindrical or prismatic, for an energy storage cell, comprising: (i) a cell body with an opening, and wherein a first coupling device is arranged on the cell body in the region of the opening; (ii) a cover with a second coupling device, wherein the cover is designed to close the opening of the cell body; (iii) wherein the first coupling device and the second coupling device are designed to bring about a positive and detachable connection by means of mutual engagement, by means of which the cell body can be closed.

1. The name of the design product: cell culture vessel (with pipeline). 2. The use of the design product: the design product is used for cell in vitro culture. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: design 1 perspective view 1. 5. Design 1 is designated as the basic design.

The application provides a scophthalmus maximus intestinal tissue cell line, which has a preservation number of CCTCC NO: C2022231. The scophthalmus maximus intestinal tissue cell line provided by the application can be continuously subcultured, has been subcultured to 60 generations and remains stable, can provide a large number of stable scophthalmus maximus intestinal cells, and is used for researches on immune function genes and epithelial adhesion function genes. The provided cell line has excellent traits, is a fibroblast, the cells are fusiform or irregular triangular, the cell bodies extend several irregular protrusions outward, and the cells are closely arranged. The cell line can be subcultured once every 3-4 days, has high resuscitation efficiency, and can be used for plasmid transfection, siRNA interference and various molecular biology experiments.

This invention discloses a GPU-based neuronal skeleton extraction method based on topology-simplified trunk growth, relating to the fields of neuroinformatics and 3D biomedical image processing. The method includes: threshold filtering and denoising of the input volume data, and retaining the largest connected component based on 3D connected component labeling to remove isolated noise; given the cell body center coordinates, determining the cell body region through spherical expansion and removing it from the foreground to highlight neural protrusions such as dendrites and axons; after obtaining the coarse skeleton, using GPU to perform topology simplification, and constructing a path scoring function combining path length and average normalized brightness, iteratively selecting and retaining the trunk path with the best score, enhancing trunk continuity and suppressing pseudo-branches caused by noise and breaks. This invention can achieve robust skeleton extraction in 3D data with noise interference and uneven brightness, and is suitable for applications such as neuronal morphology reconstruction and quantitative analysis.

The utility model discloses electrolytic hydrogen production equipment capable of alarming water leakage, which relates to the technical field of hydrogen production electrolytic cells and particularly comprises an electrolytic cell, a cell body in the middle of the electrolytic cell is arranged in a cylindrical protective shell, and a certain distance is reserved between the protective shell and the cell body; the two ends of the protective shell are connected with the supports at the two ends of the electrolytic cell respectively, the floating liquid level switch is installed at the bottom of the protective shell and connected with the alarm, water in the cell body can be concentrated at the bottom of the protective shell after leaking, and the floating liquid level switch is conducted along with rising of the liquid level to enable the alarm to give an alarm. When the water tank is used, water flowing out of the tank body flows into the horizontal water collecting area through the inclined plane, so that the water is quickly collected to lift the floating liquid level switch, and the alarm is turned on to give an alarm, so that water leakage of the tank body can be quickly found.

This invention discloses a carbon emission prediction method, system, medium, and device based on a dendritic neuron model, belonging to the field of carbon emission prediction technology. The method includes: collecting multi-source heterogeneous carbon emission data from a target region; sequentially performing data cleaning, data standardization, and data feature selection on the multi-source heterogeneous carbon emission data to obtain multi-dimensional features for the time period to be predicted; inputting the multi-dimensional features of the time period to be predicted and the hidden state of the previous time period into a pre-trained improved dendritic neuron model to obtain the final predicted value of carbon emissions; the improved dendritic neuron model includes a synaptic layer, a dendritic layer, a membrane layer, and an improved cell body layer. This invention can effectively improve the feature analysis accuracy of multi-source heterogeneous data, thereby more accurately predicting carbon emissions.