77 results about "Cell bodies" patented technology

The present invention discloses an in-situ infrared electrochemical cell for analysis and detection, which is used to be placed inside an infrared spectrometer and connected to the infrared spectrometer for use. The in-situ infrared electrochemical cell includes a cell body for containing electrolyte and a base for fixing the cell body; the cell body includes a left cell body, a right cell body, and a connection portion connecting the left cell body and the right cell body; a counter electrode is provided on the top of the cell body, and a reference electrode is provided on the top of the right cell body; the base is located directly below the right cell body, and a receiving space with an upward opening is provided inside the cell body, which can be used to partially accommodate the reaction chamber of the right cell body, and a working electrode is provided on the side of the base. Through the above-mentioned method, an in-situ infrared electrochemical cell is provided that can achieve electrolyte convection balance, low electrolyte consumption, high detection sensitivity, and simple and quick assembly and cleaning of the electrochemical cell. The in-situ infrared electrochemical cell is used for analysis and detection to achieve ultra-sensitive quantitative detection of metal ions.

A neurite growth stimulant is a small molecule ENPP1 inhibitor. Small molecule ENPP1 inhibitors are capable of treating central nervous system (CNS) injury and promoting axonal regeneration. The ENPP1 inhibitor in the carrier can be administered at damaged nerves and their cell bodies to promote the regeneration of axons.

Methods of treating epilepsy are provided. Accordingly, there is provided a method of treating epilepsy in a subject in need thereof, the method comprising: (a) administering into a thalamus nucleus of the subject a therapeutically effective amount of a polynucleotide encoding a bistable type II opsin attached to a heterologous ER export signal and / or membrane trafficking signal which enables trafficking of said bistable type II opsin to axonal presynaptic terminals; and (b) exposing a neural region of said subject to light in a wavelength that activates said bistable type II opsin, wherein said neural region comprises a cell body and / or an axon of said thalamus nucleus.

Provided are a universal cell system, a construction method therefor, and use thereof. Universal mesenchymal stem cells are constructed by means of the combination of electroporation and CRISPRoff gene editing, and the cells can survive stably in a pulmonary inflammatory pathological environment to provide sustained treatment, significantly improve multiple indicators of acute pneumonia, solve immune rejection, and provide new cell therapies and ideas for acute pneumonia. CRISPRoff is introduced into universal CTL cells by means of electroporation technology, targeting a specific region and inhibiting the expression of HLA-I in T cells; thus, T cells with low immunogenicity are constructed, and the cells can avoid allogeneic immune rejection. The universal cell system combines the advantages of both transient transfection and CRISPRoff gene editing, has great significance in the field of cell therapy, provides a more comprehensive and effective solution for the treatment of various diseases, and has a wide application prospect.

The application discloses an engineered umbilical cord stem cell and application thereof in preparation of anti-aging drugs and medical beauty products. The application constructs an engineered cell system with high anti-aging function by designing a fusion polypeptide TAT-SIRT1-SOD (SEQ ID NO: 1) and an anti-p16INK4a monoclonal antibody (mAb-p16) to cooperatively modify the umbilical cord stem cell, and combining a 3D dynamic culture technology. The fusion polypeptide is mediated to cross the membrane by a TAT cell-penetrating peptide, activates SIRT1 and removes mitochondrial ROS; the mAb-p16 is specifically combined with p16INK4a on the surface of a senescent cell, and removes the senescent cell through an ADCC effect. Experiments show that the engineered umbilical cord stem cell can increase the removal rate of the senescent cell by 86.1%, increase the SOD activity by 300%, significantly inhibit the secretion of an inflammatory factor IL-6, and increase the skin collagen density by 148% and reduce the wrinkle depth by 67% in an animal model. The application provides a novel and efficient technical scheme for anti-aging drugs and medical beauty products.

The invention relates to the technical field of dendritic cell in-vitro induction methods, and provides a dendritic cell body and an in-vitro induction method and application thereof. Thymic stromal lymphopoietin; the invention relates to a recombinant human FLT3L-Fc fusion protein. And maturing stimulation combination. On the premise of avoiding complex use of equipment, the bottlenecks of DC yield, subgroup purity and functionality are broken through.

The invention provides an electrochemical in-situ reaction device for time-resolved XAFS (X-ray fluorescence spectroscopy) testing, which relates to the field of spectral analysis testing and comprises a reaction tank body, a liquid layer thickness adjusting mechanism and a visualization mechanism, the end face sealing mechanism is arranged on the side, away from the first through hole, of the second through hole and is provided with a sample mounting position; an optical window hermetically extending into the reaction tank body is formed at one end of the visualization mechanism, and the liquid layer thickness adjusting mechanism adjusts the distance between the optical window and the second through hole; when the end face sealing mechanism is hermetically connected to the reaction tank body, the sample mounting position faces the interior of the tank body. According to the invention, the distance between the optical window and the second through hole is precisely adjusted through the liquid layer thickness adjusting mechanism to precisely adjust the thickness of the liquid layer, so that the application of the millisecond-level time-resolved XAFS technology in the electrochemical liquid phase reaction direction is realized, and a high-precision in-situ reaction device is provided.

The application relates to a method and system for simultaneously obtaining complete morphology and omics molecular information of a single neuron, which comprises the following steps: obtaining a sample in which the cell body, dendrite and axon of a neuron are labeled by fluorescence; performing a cycle operation of scanning imaging and cutting on the section of the sample until the scanning imaging of the whole sample is completed, and collecting the cut section containing the fluorescence-labeled neuron cell body; based on the three-dimensional image of the sample section obtained by the scanning imaging operation, reconstructing the complete morphology of the neuron, and extracting the fluorescence-labeled neuron cell body from the collected section to obtain the omics molecular information of the neuron by omics analysis. The application can effectively integrate the fine complete morphology and omics molecular information of the same neuron, and successfully solves the problem that the two key information cannot be simultaneously obtained at the single neuron precision level.

A method of manipulating and / or investigating cellular bodies (9) is provided. The method comprises the steps of: providing a sample holder (3) comprising a holding space (5) for holding a fluid medium (11); providing a sample (7) comprising one or more cellular bodies (9) in a fluid medium (11) in the holding space (5); generating an acoustic wave in the holding space exerting a force (F) on the sample (7) in the holding space (5). The method further comprises providing the holding space (5) with a functionalised wall surface portion (17) to be contacted by the sample (7) and the sample (7) is in contact with the functionalised wall surface portion (17) during at least part of the step of application of the acoustic wave. A system and a sample holder (3) are also provided.

A vapor cell comprises a cell body and a cell window, which is anodically connected to the cell body. The cell window and the cell body define a vapor cavity designed to contain a vapor comprising a variety of alkali atoms.

This invention discloses a compact high-temperature, high-pressure transmission XAFS in-situ cell, comprising a cell body and a cover that are sealed together. A light-transmitting window assembly is installed on the cell body, allowing X-rays to pass through and irradiate the sample to be tested, which is installed inside the cell body. The cell body and cover are also connected to and equipped with components such as a ball valve, a gas connection, a temperature probe, test electrodes, and a heating element. The heating element is used to regulate the sample temperature, and a water-cooling structure located in the side wall of the cell body is used to regulate the cell body temperature. This invention integrates a ball valve control structure on the same cell body. When the ball valve is closed, a high-pressure atmosphere can be established and maintained inside the cell body to achieve high-temperature, high-pressure transmission XAFS experiments; when the ball valve remains open, the inside of the cell body is in a normal-pressure gas flow state, achieving high-temperature, normal-pressure transmission XAFS experiments. One device can meet the testing requirements of two different operating conditions, significantly reducing the user's equipment investment costs.

The present application relates to the technical field of kits, in particular to a kind of NK cell in vitro expansion kit, including NK cell kit body, NK cell kit body is equipped with separating plate in the middle, the two sides of separating plate are reagent bottle storage cavity and cell culture cavity respectively, the top of reagent bottle storage cavity is equipped with first isolation plate, and the top of cell culture cavity is equipped with second isolation plate.The present application is equipped with reagent bottle storage cavity and cell culture cavity in NK cell kit body, which can facilitate the culture of cells, and facilitate the storage of reagent bottles;By being equipped with jacking block in reagent bottle storage cavity, the reagent bottle in storage groove can be jacked up and ejected from the storage groove by the upward movement of jacking block driven by the downward pressure of pressure rod, so that the reagent bottle can be easily taken, and the operability is strong;By being equipped with magnetic baffle in the symmetry in universal ball, after the dropper is pulled out of the through hole, the magnetic baffle can be automatically closed under the action of magnetic force.

The utility model relates to a filtering assembly, which belongs to the technical field of air filtering equipment, and comprises at least two cell bodies, first buckles and second buckles, the two ends of the top of each cell body are provided with first clamping holes, and the two ends of the bottom of each cell body are provided with second clamping holes. The first buckle is clamped between the two opposite first clamping holes of the two adjacent cell bodies in a matched mode, the second buckle is clamped between the two opposite second clamping holes of the two adjacent cell bodies in a matched mode, and by the adoption of the structure, the multiple cell bodies are connected in a buckled mode through the first buckle and the second buckle; the buckle is arranged in the filtering section of the air filter, the buckle is limited by the inner wall of the filtering section, the buckle is prevented from being released, the connection is more reliable, the cell body can be quickly disassembled by opening the buckle when maintenance and cleaning are needed, and the working efficiency is improved.

The application discloses a multi-cell horizontal distance-variable module, relates to a cell distance-variable conveying technology, and comprises a bearing base and a conveying base arranged in front of the bearing base, and a track conveying module is arranged above the conveying base and used for driving a cell body to move and convey; the top surface of the bearing base is provided with a transverse slider, and a transverse sliding frame is slidably arranged in the transverse slider; the longitudinal slider is slidably arranged in the longitudinal sliding frame, and a distance-variable mechanism is arranged at the front of the upper end of the longitudinal sliding frame; and the distance-variable mechanism comprises a clamping side frame. The multi-cell horizontal distance-variable module is driven by the transverse slider and the longitudinal slider to move, meanwhile, a plurality of distance-variable side frames are adapted to cell bodies of different specifications to adjust the distance, and a supporting mechanism above the conveying track module is used to assist the cell body to move, so that the cell body is prevented from falling and affecting the processing efficiency during distance-variable conveying operation.

The invention discloses engineered umbilical cord stem cells and application thereof in preparation of anti-aging drugs and medical beauty products. A fusion polypeptide TAT-SIRT1-SOD (SEQ ID NO: 1) and an anti-p16INK4a monoclonal antibody (mAb-p16) are designed to synergistically modify umbilical cord stem cells, and a 3D dynamic culture technology is combined to construct an engineered cell system with an efficient anti-aging function. The fusion polypeptide mediates transmembrane through TAT cell-penetrating peptide, activates SIRT1 and removes mitochondrial ROS; the mAb-p16 is specifically combined with p16INK4a on the surface of the senescent cell, and the senescent cell is eliminated through an ADCC effect. Experiments show that the engineered umbilical cord stem cell can increase the senescent cell clearance rate by 86.1%, increase the SOD activity by 300%, significantly inhibit secretion of inflammatory factors IL-6, increase the skin collagen density by 148% and reduce the wrinkle depth by 67% in an animal model. The invention provides a novel and efficient technical scheme for anti-aging medicines and medical beauty products.

Compositions comprising a condensed mesenchymal cell body and a hydrogel are provided. The compositions may further include drugs or growth factors. The condensed mesenchymal cell body may include a connective tissue cell, or even a progenitor cell capable of producing connective tissue extracellular matrices such collagen and glycosaminoglycan. Also provided are methods of treating connective tissue defects, cartilage injury, and cartilage degradation.

The invention relates to a cell housing, in particular cylindrical or prismatic, for an energy storage cell, comprising: (i) a cell body with an opening, and wherein a first coupling device is arranged on the cell body in the region of the opening; (ii) a cover with a second coupling device, wherein the cover is designed to close the opening of the cell body; (iii) wherein the first coupling device and the second coupling device are designed to bring about a positive and detachable connection by means of mutual engagement, by means of which the cell body can be closed.

1. The name of the design product: cell culture vessel (with pipeline). 2. The use of the design product: the design product is used for cell in vitro culture. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: design 1 perspective view 1. 5. Design 1 is designated as the basic design.

The application provides a scophthalmus maximus intestinal tissue cell line, which has a preservation number of CCTCC NO: C2022231. The scophthalmus maximus intestinal tissue cell line provided by the application can be continuously subcultured, has been subcultured to 60 generations and remains stable, can provide a large number of stable scophthalmus maximus intestinal cells, and is used for researches on immune function genes and epithelial adhesion function genes. The provided cell line has excellent traits, is a fibroblast, the cells are fusiform or irregular triangular, the cell bodies extend several irregular protrusions outward, and the cells are closely arranged. The cell line can be subcultured once every 3-4 days, has high resuscitation efficiency, and can be used for plasmid transfection, siRNA interference and various molecular biology experiments.

This invention discloses a GPU-based neuronal skeleton extraction method based on topology-simplified trunk growth, relating to the fields of neuroinformatics and 3D biomedical image processing. The method includes: threshold filtering and denoising of the input volume data, and retaining the largest connected component based on 3D connected component labeling to remove isolated noise; given the cell body center coordinates, determining the cell body region through spherical expansion and removing it from the foreground to highlight neural protrusions such as dendrites and axons; after obtaining the coarse skeleton, using GPU to perform topology simplification, and constructing a path scoring function combining path length and average normalized brightness, iteratively selecting and retaining the trunk path with the best score, enhancing trunk continuity and suppressing pseudo-branches caused by noise and breaks. This invention can achieve robust skeleton extraction in 3D data with noise interference and uneven brightness, and is suitable for applications such as neuronal morphology reconstruction and quantitative analysis.

This invention discloses a carbon emission prediction method, system, medium, and device based on a dendritic neuron model, belonging to the field of carbon emission prediction technology. The method includes: collecting multi-source heterogeneous carbon emission data from a target region; sequentially performing data cleaning, data standardization, and data feature selection on the multi-source heterogeneous carbon emission data to obtain multi-dimensional features for the time period to be predicted; inputting the multi-dimensional features of the time period to be predicted and the hidden state of the previous time period into a pre-trained improved dendritic neuron model to obtain the final predicted value of carbon emissions; the improved dendritic neuron model includes a synaptic layer, a dendritic layer, a membrane layer, and an improved cell body layer. This invention can effectively improve the feature analysis accuracy of multi-source heterogeneous data, thereby more accurately predicting carbon emissions.

1. The name of the design product: cell culture vessel. 2. The use of the design product: the design product is used for cell culture in vitro. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: design 1 perspective view 1. 5. Design 1 is designated as the basic design.

The present invention relates to the field of electroplating technology, specifically a nickel-plating electrolytic cell, comprising: a cell body, the cell body being fixedly installed horizontally on the ground; a protective device for preventing harmful gases from being released into the air is provided above the cell body, the protective device comprising: a slide rail, two slide rails being laterally provided on the upper side of the cell body, a cover being provided on the two slide rails, a fixed plate being fixedly installed on both sides of the two cover shells, each fixed plate being fixedly installed with two sliders, eight sliders being slidably installed on the slide rails, and two cylinders being vertically and symmetrically installed on the top of the two cover shells. By adding a cover shell and a partition, the present invention reduces the time that the cell mouth of the electrolytic cell is exposed to the air while ensuring that electroplating can be effectively completed, thereby greatly reducing the release of harmful gases volatilized by the heated electrolyte into the air, reducing the harm of harmful gases to the human body, and effectively protecting the life and health of workers.

The utility model relates to a multi-module combination migration test pool, which comprises a migration pool body, a bottom plate and a cover plate, the migration pool body is arranged above the bottom plate and is detachably connected with the bottom plate, the cover plate is arranged above the migration pool body, a partition plate is arranged in the migration pool body and divides an inner cavity of the migration pool body into a plurality of test modules, and the test modules are connected with the bottom plate. A circle of pool body clamping groove is formed in the bottom plate, the size of the pool body clamping groove is matched with that of the migration pool body, the bottom of the migration pool body can be conveniently inserted into the pool body clamping groove to be clamped, a partition plate clamping groove is formed in the pool body clamping groove and matched with the partition plate, and the bottom of the partition plate is clamped in the partition plate clamping groove; the cover plate is provided with a subarea matched with the test module, and the cover plate is detachably connected with the bottom plate. According to the utility model, a migration test can be carried out on a plurality of samples simultaneously, and the test efficiency is improved.

The application relates to the technical field of brain science image processing, and discloses a brain tissue neuron morphology three-dimensional reconstruction and connection group analysis method and system. The method comprises the following steps: feature point extraction and matching and optical flow registration are performed on continuous ultrathin section electron microscope images to obtain three-dimensional image body data; a three-dimensional deep convolutional neural network is used for semantic segmentation of cell bodies, dendrites, axons and dendritic spines, and secondary registration is driven through confidence feedback; synapses are detected and classified into excitatory or inhibitory types; topological perception skeletonization reconstruction is performed, and segmentation correction is constrained through topological anomaly feedback; and a synapse connection matrix is constructed, and graph theory analysis is performed.

A detection cell for a chromatography system, the detection cell including: a cell body including a counter electrode in the form of a cell body or wire; a working electrode block including a working electrode; a gasket separating the working electrode block from the cell body, the gasket extending between an inlet and an outlet of the detection cell and defining a sample flow path in fluid contact with the cell body, the counter electrode, and the working electrode; and a reference electrode system in fluid contact with the outlet and including a platinum auxiliary electrode operably connected to a positive pole of a power source and a platinum reference electrode operably connected to a negative pole of the power source.

The invention belongs to the technical field of optical measurement and atomic precision devices, and discloses a caesium reference cell for an optical cavity ring-down system and the optical cavity ring-down system, the caesium reference cell comprises a cell body, the cell body comprises an inner cell body and an outer cell body, the inner cell body is used for containing caesium elements and generating caesium steam, and the outer cell body surrounds the inner cell body to provide a heat preservation environment; the temperature control assembly comprises a heating unit and a refrigerating unit, the heating unit is used for increasing the temperature of the inner pool body, and the refrigerating unit is used for forming a cold end on the inner pool body; the optical windows are arranged on the two opposite sides of the pool body and used for allowing a target light beam to penetrate through the cesium steam; and the control unit is used for determining the absolute cesium particle number concentration in the inner tank body according to the temperature of the inner tank body and the light beam penetrating through the cesium steam.

The invention relates to the field of biological hospitals, and discloses a tumor follicular helper T cell strain for vascular immune mother cell T cell lymphoma as well as a construction method and application of the tumor follicular helper T cell strain. The invention discloses an AITL tumor TFH cell strain, the name of which is human tumor follicular helper T cell AITL-CH1. The strain is preserved in CCTCC; the preservation date is 2025 to 08 to 12; the preservation number is CCTCC (China Center For Type Culture Collection) NO: C2025233 The construction method comprises the steps of isolated culture of primary cells, in-vitro induction culture of TFH cells and isolated passage. The tumor TFH cell strain of the AITL patient is successfully established, the cell strain has stable TFH characteristic phenotype and good multiplication capacity, the limitation of short in-vitro culture period of primary cells is effectively overcome, and a foundation is laid for in-depth study on AITL epigenetic modification and molecular pathogenic mechanism. A reliable and stable cell model is provided for clinical immunotherapy and targeted drug research and development.

The utility model discloses a water electrolysis hydrogen production electrolytic bath with an elastic support body, which comprises a bath body, a diaphragm is arranged on the inner wall of the bath body, a first electrode net and a first pole plate are respectively arranged on one side of the diaphragm, and the first electrode net and the first pole plate are respectively arranged on the inner wall of the bath body. And a second electrode net and a second polar plate are arranged on one side, far away from the first electrode net, of the diaphragm. The utility model relates to the technical field of water electrolysis hydrogen production, in particular to a water electrolysis hydrogen production electrolytic bath with an elastic support body, which realizes that a first vertical plate and a second vertical plate are propped against two polar plates and two electrode nets through the matching among a transverse column, the first vertical plate, the second vertical plate and a concave hole, and solves the problem that when the existing elastic support body is used, the first vertical plate and the second vertical plate are not tightly pressed against the two polar plates and the two electrode nets. Due to the fact that the contact area between the spring and the polar plate and the contact area between the spring and the electrode net are small, the polar plate and the electrode net can be infirmly contacted, and the polar plate and the electrode net cannot abut against each other.

Compositions comprising a condensed mesenchymal cell body and a hydrogel are provided. The compositions may further include drugs or growth factors. The condensed mesenchymal cell body may include a connective tissue cell, or even a progenitor cell capable of producing connective tissue extracellular matrices such collagen and glycosaminoglycan. Also provided are methods of treating connective tissue defects, cartilage injury, and cartilage degradation.