The invention belongs to the technical field of analysis and detection, and discloses a preparation method of an Eu-MOF based
ratiometric fluorescence sensor and application of the Eu-MOF based
ratiometric fluorescence sensor in portable detection of
natamycin. The preparation method comprises the following steps: (1) dispersing 2, 5-dihydroxyterephthalic acid,
pyromellitic acid and ZrCl4 in deionized water, then adding
acetic acid, heating to react for a period of time, and then cooling to obtain a suspension; (2) transferring the obtained suspension into another reaction container, adding deionized water, continuously reacting, cooling after the reaction is completed, sequentially centrifuging with water, DMF (
Dimethyl Formamide) and
ethanol, and finally performing
vacuum drying overnight to obtain an off-white
solid UiO-66 (OH) 2 / (COOH) 2; and (3) dispersing EuCl3. 6H2O and the obtained UiO-66 (OH) 2 / (COOH) 2 in deionized water, carrying out heating reaction, cooling after the reaction is completed, centrifuging the obtained product, washing with water and
ethanol, and finally, carrying out
vacuum drying on the generated precipitate overnight to obtain an off-white
solid Eu-coated UiO-66 (OH) 2 / (COOH) 2. The obtained Eu (at) UiO-66 (OH) 2 / (COOH) 2 has the advantages of high sensitivity,
high selectivity, ratio
fluorescence response and the like on NAT.