28results about How to "Great potential" patented technology

The application belongs to the technical field of biological medicine, and particularly relates to a kind of lipid nanoparticles and engineered immune cells. The application first provides an engineered immune cell, the engineered immune cell includes immune cell, lipid nanoparticle and exogenous drug;The exogenous drug is loaded in the lipid nanoparticle;When the immune cell is contacted with the lipid nanoparticle, the immune cell and the lipid nanoparticle endocytosis occur to obtain the engineered immune cell. In another aspect, the application also provides a kind of lipid nanoparticles that can improve the encapsulation efficiency of mRNA and the delivery efficiency of immune cells.

The application discloses a quaternary solid waste-based soil stabilizer (GSCP) for a base layer, which is composed of slag (GGBFS), steel slag (SSA), calcium carbide slag (CS) and phosphogypsum (PG) and has a weight ratio of GGBFS:SSA:CS:PG=56:24:12:8. The stabilizer forms C-S-H colloid and ettringite (AFt) through alkaline activation and sulfate activation, does not need to add cement or chemical activator, is used for stabilizing silty clay and steel slag mixed soil, and significantly improves the unconfined compressive strength, splitting tensile strength, water stability and freeze-thaw resistance. Compared with a traditional cement-based stabilizer, the application reduces carbon emission by 82% and cost by 31.25%, provides a low-carbon and economical solution for road engineering, and is suitable for the base layer and the subbase layer of a secondary road or above.

The application discloses a method for reprogramming Müller glial cells (MG) to induce cell proliferation and neuron regeneration, and belongs to the technical field of biological medicine. The application constructs an FGF2 / Ascl1 / RA combined reprogramming system. The system induces MG to re-enter the cell cycle under the condition of no neurotoxic injury through FGF2 combined with AAV-Ascl1, and promotes the multi-round proliferation of adult and middle-aged and old MG by using FGF2 / MAPK / PI3K-Akt signals, and further combines with RA to induce MG to maintain a regeneration state for a long time and promote neurogenesis. The reprogramming system established by the application successfully solves a plurality of core technical problems in the field of mammalian retina regeneration. This breakthrough not only provides a brand-new strategy for the regenerative medicine intervention of retina degenerative diseases, but also shows great potential and value of the application from basic research to clinical transformation application, so that the application has a good application prospect.

The application discloses a conjugated polymer of chlorobenzene, o-dichlorobenzene or m-dichlorobenzene and EDOT as well as a preparation method and application thereof, and belongs to the field of polymer electrochromic materials. By introducing chlorine atoms as intermediates in different active sites of benzene and EDOT as a strong electron-donating group, the obtained conjugated polymer has the characteristics of a wider redox peak, good optical contrast, fast response time, high coloring efficiency, good stability and the like.

The application relates to a hydrogen production system. The hydrogen production system comprises an electrolysis cell module and a steam heat pump module. The steam heat pump module comprises a heat pump assembly, a water storage tank and a flash tank. The heat pump assembly comprises a condenser. An outlet of the water storage tank is connected to a cold side inlet of the condenser. A cold side outlet of the condenser is connected to an inlet of the flash tank. An outlet of the flash tank is connected to an inlet of a fuel gas preheater. The steam heat pump module of the application only needs a small amount of electric energy to generate a large amount of water vapor. In the case of the same water vapor yield, the power consumption of the steam heat pump module is only about half of the power consumption of the water vapor generator, achieving a more energy-saving effect. In the case of the same power, the water vapor yield of the steam heat pump module is much larger than the water vapor yield of the steam generator, so that the hydrogen production system of the application can meet the demand of a high-power hydrogen production system.

This invention belongs to the field of fungal detection technology, specifically relating to a micro-platform for rapid electrolysis and highly sensitive electrochemical nucleic acid detection of Cryptococcus neoformans and its application. The micro-platform includes an integrated microfluidic chip, an external power supply, a sample introduction system, and / or an imaging system. The integrated microfluidic chip includes an electrolysis region, an electrochemical detection region, and an electrode array region. The electrolysis region includes multiple vertically arranged counter electrodes arranged in pairs, and multiple horizontally arranged hollow channels in a serpentine pattern, bonded to the electrode pairs above them. The connection point between every two electroporated channels is the electroporation lysis point. The electrochemical detection region consists of a working electrode, a reference electrode, and a counter electrode. The working electrode is modified with rGO / AuNPs nanomaterials and molecular probes.

The invention provides a 3, 5-dihydroxy-4 '-pentylbiphenyl-betaine eutectic as well as a preparation method and application thereof, and belongs to the technical field of pesticide preparation. The invention provides a 3, 5-dihydroxy-4 '-pentylbiphenyl-betaine eutectic which is formed by combining 3, 5-dihydroxy-4'-pentylbiphenyl and betaine through an intermolecular hydrogen bond, and a preparation method of the 3, 5-dihydroxy-4 '-pentylbiphenyl-betaine eutectic. According to the 3, 5-dihydroxyl-4 '-pentylbiphenyl-betaine eutectic and the preparation method thereof, the eutectic is formed by BP and BET, the solubility and the dissolution rate of BP are improved, the application amount of pesticide molecules is reduced, and the 3, 5-dihydroxyl-4'-pentylbiphenyl-betaine eutectic is applied to various plant pathogenic fungi. Results of the embodiment show that the co-crystals formed by BP and BET are combined through hydrogen bonds in a molar ratio of 1: 1, the dissolution effect of the co-crystals is superior to that of the medicine, and the dissolution effect is not affected by the pH environment. Compared with drugs with the same concentration and the drug eutecticum, the drug eutecticum shows the antibacterial activity advantage superior to that of original drug molecules.

The invention relates to the technical field of gene engineering detection, and particularly discloses a gene detection system for promoting andrographolide synthesis based on a biosensor, a key gene ApDof29 for regulating and controlling andrographolide synthesis is determined through whole genome analysis, gene expression profile research and co-expression network analysis, the core regulation and control effect of ApDof29 is verified, and the gene detection system for promoting andrographolide synthesis based on the biosensor is used for promoting andrographolide synthesis. The invention also develops a real-time dynamic monitoring system, and realizes non-invasive monitoring of andrographolide content accumulation, biosynthesis rate and metabolic intermediate concentration change by utilizing the fusion of a specific response element and a reporter gene. Through comprehensive calculation processing of an accumulation abnormal coefficient, a synthesis rate abnormal coefficient and a concentration change abnormal coefficient, the regulation and control effect of ApDof29 is dynamically evaluated, and a scientific basis is provided for subsequent gene modification.

The application provides a preparation of gramicidin and an antibacterial hydrogel preparation of the gramicidin, and discloses medical uses of the gramicidin, and provides a recombinant gramicidin, heterologous expression of the gramicidin is realized by constructing a Pichia pastoris recombinant strain, and the antibacterial activity of the gramicidin is retained, and the method has the advantages of simplicity and rapidness. Meanwhile, the hydrogel preparation of the gramicidin provided by the application shows good potential for treating wound infection of a diabetic mouse, and has the performance of rapid adhesion and hemostasis, lays a foundation for development of a new antibacterial drug, and provides a new direction for treatment of chronic inflammatory wounds and open bleeding.

The application provides a pantoea vagans E-102, a microbial inoculant and application thereof, and belongs to the technical field of microorganisms. Pantoea vagans The Latin name of the pantoea vagans E-102 is CCTCC NO:M 20261185. The pantoea vagans E-102 has excellent antagonistic activity on various pathogenic fungi. The plate confrontation test shows that the strain can significantly inhibit the growth of verticillium albo-atrum, fusarium graminearum, pyricularia oryzae, botrytis cinerea and sclerotinia sclerotiorum; the volatile substances also have strong bacteriostatic effect on pathogenic fungal hyphae. The indoor potting test proves that the strain has significant prevention effect on cotton verticillium wilt; the 90-day outdoor potting test further proves that the strain has stable and long-acting prevention and treatment capacity on cotton verticillium wilt. In conclusion, the pantoea vagans E-102 has good application prospect in the field of biological control of fungal diseases of cotton and other crops.

The invention discloses talaromyces pinophilus and application thereof. Belongs to the technical field of biological control. According to the invention, a strain HY25 capable of efficiently antagonizing seven kinds of tobacco fusarium is separated from rhizosphere of a diseased plant by a tissue isolation method and a plate confrontation method. The talaromyces pinophilus is identified as talaromyces pinophilus through morphological and molecular biological tree establishment. A further research shows that the strain HY25 has various growth-promoting and disease-preventing capacities of dissolving potassium, dissolving phosphorus, fixing nitrogen and the like. Pot experiment results show that the control effect of fermentation liquor of the strain HY25 on tobacco fusarium root rot is 75%, and the strain HY25 has a remarkable growth promoting effect on indexes such as plant height, root length, fresh weight and leaf surface area of tobacco. In conclusion, the strain HY25 has good biological prevention and control potential on the tobacco fusarium root rot, has a growth promoting effect, and enriches biocontrol strain resources of the tobacco fusarium root rot.

The application discloses an in-situ vacuum preparation system and a preparation method of a memristor, and belongs to the technical field of electronic device manufacturing. The in-situ vacuum preparation system comprises a glove box, and a vacuum collecting and connecting pipeline is connected to one side of the glove box; the vacuum collecting and connecting pipeline can be used for carrying out the following processes respectively: insulating material thermal evaporation, metal material thermal evaporation, reactive ion etching, ion beam etching, electron beam evaporation and magnetron sputtering; the insulating material evaporation and the electron beam evaporation both adopt an electron beam evaporation device; the metal material thermal evaporation and the magnetron sputtering both adopt a magnetron sputtering device; the reactive ion etching adopts a reactive ion etching device; and the ion beam etching adopts an ion beam etching device. Material deposition and electrode manufacturing in the preparation process of the memristor are carried out in the in-situ vacuum preparation system, material pollution and interface defects are avoided, the performance of the memristor is significantly improved, and the preparation process is simplified.

This invention discloses an iron-containing diatomaceous earth shell material, its preparation method, applications, and a method for preparing a tannic acid-diatomaceous earth shell composite material containing this material. The iron-containing diatomaceous earth shell material contains at least 0.4% iron by weight and at least 88% silicon, retaining the highly ordered nanoscale porous structure of natural diatomaceous earth shells. The preparation method involves culturing diatoms in G11 medium supplemented with sodium silicate nonahydrate and ferrous sulfate heptahydrate, followed by extraction via hydrogen peroxide oxidation, acid washing, and high-temperature calcination. This invention further utilizes the reaction of this iron-containing diatomaceous earth shell with tannic acid in a buffer system at pH 7.0-8.0, achieving efficient loading of tannic acid through the coordination of iron ions with tannic acid and physical adsorption. This invention features a simple process, low cost, and environmental friendliness. The prepared composite material significantly improves the chemical stability and bioavailability of tannic acid, showing broad application prospects in biomaterials fields such as drug delivery, antibacterial applications, and tissue engineering.

The ZIF-8 / algae powder-based composite aerogel, preparation method and application thereof relate to the field of environmental engineering and material science technology. A novel chitosan / ZIF-8 / algae powder (ZIF-8 / CP / CS) composite adsorbent is synthesized for efficient adsorption of uranium ions in seawater. The results show that the adsorbent has good adsorption performance for uranium in the pH range of 4.0-8.0, and the adsorption rate is all above 89%. Mechanism analysis shows that the hydroxyl, amino, phosphoric acid groups on the surface of ZIF-8 / CP / CS and the C=N bond in ZIF-8 play a key role in the adsorption of uranium, and the quaternary phosphonium salt improves the competitiveness of the adsorbent for uranium. The competitive adsorption experiment shows that compared with the original ZIF-8 and ZIF-8 / C / CS without immobilized ionic liquid, the uranium vanadium ratio of ZIF-8 / CP / CS is increased by 20 times and 6 times respectively. The real uranium tailing pond wastewater is treated by adsorption, and the uranium content of the wastewater is reduced to 40 μg / L, so the adsorbent has good potential for extracting uranium from seawater and good application prospect for recovering uranium from uranium tailing wastewater.

This invention discloses a method and its application for enhancing the antibacterial activity of *Propionibacterium f. reudenreichii* subsp. *shermanii* metabolites by adding carrageenan to a semi-solid culture medium. The method uses *Propionibacterium f. reudenreichii* subsp. *shermanii* NBRC No. 12426 as the experimental strain and *Pichia anomala* SICC 2.894 as the indicator strain. It discloses a method for enhancing the antibacterial activity of *Propionibacterium f. reudenreichii* subsp. *shermanii* metabolites against the indicator strain *Pichia anomala* by adding carrageenan to the culture medium. Based on the culture conditions and method of *Propionibacterium f. reudenreichii* subsp. *shermanii* in this invention, the optimal amount of carrageenan added is selected as 0.6%, which significantly enhances the antibacterial activity of the *Propionibacterium f. reudenreichii* subsp. *shermanii* metabolites.

The utility model provides an integrated micro plastic wastewater treatment and its resource utilization device, including coagulation tank, dewatering machine, ball mill, calcinator, premixing pool and fluidized bed, coagulation tank, dewatering machine, ball mill, calcinator are connected in proper order, coagulation tank, premixing pool, fluidized bed are connected in proper order, the utility model discloses through coagulation tank to the effective enrichment of micro plastic, subsequently transforms into heterogeneous fenton catalyst, fills in fluidized bed, is used for the purification removal of organic matter wastewater, the utility model discloses device reasonable in design, and simple in use process, not only can satisfy the efficient treatment of micro plastic wastewater, realizes the resource utilization of micro plastic waste simultaneously, has higher economic value and application prospect, and is suitable for industrialization large -scale production.

The invention discloses an mRNA (messenger ribonucleic acid) delivery system based on a nucleic acid nanostructure as well as a preparation method and application of the mRNA delivery system. The mRNA delivery system comprises an mRNA scaffold chain, an RNA staple chain, a lipid-nucleic acid coupling chain and an auxiliary lipid; the mRNA scaffold chain comprises a nucleic acid sequence for coding target protein mRNA; the mRNA scaffold chain and the RNA staple chain are self-assembled through complementary base pairing to form a nucleic acid nanostructure; the lipid-nucleic acid coupling chain comprises a lipid molecule and a nucleic acid chain coupled with the lipid molecule. According to the invention, mRNA molecules of coding functional protein are used as scaffold chains to be accurately integrated into the nucleic acid nanostructure, so that the purpose of protecting and delivering mRNA is achieved, and the effect of accurately regulating and controlling the expression of target protein is achieved.

This invention discloses a detection method for ultrasensitive fluorescence counting immunoassay using bioorthogonal reaction-immobilized bacteria, comprising the following steps: (1) transforming recombinant plasmids into *E. coli*, and inducing transcription into the T promoter using IPTG to induce *E. coli* to express green fluorescent protein; (2) dissociating Cu-MOF nanoparticles with ascorbic acid, allowing the azide groups of *E. coli* modified with azidoform to bind to the alkyne groups on alkyne-modified bovine serum albumin, thus immobilizing *E. coli* on a well plate; (3) amplifying the signal through the self-replication of luminescent *E. coli*, converting the concentration of the biomarker into fluorescent bright spots of fluorescent *E. coli*, thereby achieving quantitative detection. This invention has strong detection specificity, good stability and accuracy, and a detection limit lower than the clinical gold standard ELISA, allowing for visual detection of biomarker concentrations.

The application splits the molecular structures of phenylpyrimidine and benzamide, and discloses aniline pyrimidine compounds by means of computer-aided drug design. Research results show that the aniline pyrimidine compounds have PLK1 inhibitory activity and have obvious inhibitory effect on cancer cells. The application also provides a preparation method of the aniline pyrimidine compounds. The method is scientific and reasonable, simple and easy to operate, has few reaction steps, high yield and is suitable for industrial production.

The present application relates to a kind of drug delivery system based on nucleic acid chemical modification and its preparation method and application.The drug delivery system includes nucleic acid aptamer coupling drug and antisense nucleic acid coupling drug, and the nucleic acid aptamer coupling drug and antisense nucleic acid coupling drug are co-assembled to form nanoparticle;The nucleic acid aptamer coupling drug includes nucleic acid aptamer and the chemotherapeutic drug coupled therewith;The antisense nucleic acid coupling drug includes antisense nucleic acid and the photosensitizer coupled therewith.The present application can reduce the up-regulation of the expression level of anti-apoptotic gene caused by chemotherapy and photodynamic therapy in tumor site by assembling tumor-targeting nucleic acid aptamer, chemotherapeutic drug, photosensitizer and antisense nucleic acid into drug delivery system, realizes the combination of chemotherapy, photodynamic therapy and gene therapy, efficiently inhibits tumor growth, and preparation method is simple and easy to operate, with universality, large drug loading, high production efficiency, it is expected to be applied in the treatment of various diseases, and promote the popularization and use of related products.

According to the fugu rubripes seedling raising and cultivating method, through the synergistic effect of screening and constructing disease-resistant germplasm and developing functional feed, the dual technical problems of unstable disease resistance and antibiotic dependence in traditional cultivation are solved, and synchronous improvement of the cultivation benefits and ecological safety of fugu rubripes is achieved. The molecular marker related to the vibrio anguillarum infection resistance of the takifugu rubripes obtained by screening is used for screening vibrio harveyi resistant parents, so that the genetic breeding speed of the takifugu rubripes is increased, the harm of bacterial diseases to the culture of the takifugu rubripes is reduced, and the breeding cost of the takifugu rubripes is reduced. On the other hand, the invention provides an antibacterial peptide feed which can be used for breeding the fugu rubripes, and the antibacterial peptide feed has good potential as an antibiotic substitute, so that a new method is provided for healthy breeding of the fugu rubripes.

This invention discloses the preparation of a nitrogen-containing phosphate resin monomer and its application as an indicator of iron ions. The structure of the compound is shown in the figure below. Using a phosphorus-containing heptanobromoborone compound from the paper "Preparation of a Phosphorus-Containing Heptanobromoborone Compound and Its Application in Fluorescent Ink" as a raw material, the compound undergoes a hydrolysis reaction under the action of trimethylbromosilane to generate a nitrogen-containing phosphate resin monomer. The synthetic route is simple, the post-processing is convenient, and the product yield is high. This compound exhibits high fluorescence quantum yield in both organic and aqueous phases. Ultimately, this compound is used for the indication of iron ions in aqueous phases. 3+ It exhibits selective detection with high sensitivity.

The application provides an epsilon phase gallium oxide based material and a crystal student growth method and use thereof, the crystal student growth method comprising epitaxial growth of the epsilon phase gallium oxide based material on a substrate with a polar surface, so that the epsilon phase gallium oxide based material has the same polarization direction as the polar surface. The application selects a substrate with a specific surface polarity and controls the growth of high-quality crystals through substrate and template engineering, thereby guiding controllable polarity growth by using the specific substrate as a template. Thus, active regulation of the polarization direction of the epsilon phase gallium oxide based material can be supported, the bottleneck of single polarization characteristics of the material system is broken, a key material foundation for developing a new generation of high-performance functional devices based on polarization engineering is laid, and the application has important scientific and application values.

The present application belongs to the technical field of electrocatalytic materials, and particularly relates to a nano-flower-shaped three-phase electrocatalyst as well as a preparation method and application thereof. Coated cobalt nanoparticles are obtained by coating cobalt nanoparticles on carbon nanotubes grown in situ on a self-supporting substrate to obtain a CoFC substrate; nano-flower Bi2O3 with Bi2MoO6 nanoparticles is formed in situ on the CoFC substrate by electrochemical deposition to obtain a nano-flower-shaped three-phase electrocatalyst. The nano-flower-shaped three-phase electrocatalyst prepared by the present application exhibits excellent OER and HER bifunctional electrocatalytic activity in an alkaline medium. A double-electrode electrolytic cell assembled using the nano-flower-shaped three-phase electrocatalyst can reach a current density of 10 mA / cm 2 in a 1M potassium hydroxide electrolyte at only 1.50 V, and can be stably operated for more than 45 hours, exhibiting excellent overall water splitting performance and excellent stability.

The present application relates to an inhibitor compound, and aims to provide an acetohydroxy acid synthase inhibitor and a preparation method and application as a herbicide thereof. The structural formula of the compound is shown as formula (I), and the compound specifically inhibits the activity of acetohydroxy acid synthase or its mutant W574L in plants. The present application further provides a method for applying the compound as an agricultural chemical herbicide. The compound has good enzyme inhibition activity on wild-type acetohydroxy acid synthase and / or its mutant W574L, and thus can be used as an agricultural chemical herbicide for the prevention and treatment of various weeds in the agricultural field; can be used as a pre-emergence and post-emergence herbicide, and the prevention and treatment objects include various field weeds, and has a special effect on the prevention and treatment of the malignant weed Euphorbia lathyris L.; has the characteristics of low dosage, excellent herbicidal activity, wide weed spectrum, good transmission, wide application window period, safety to rice and the like.

The application provides a preparation method of a negative electrode material, a prepared negative electrode material and application thereof, and the preparation method comprises the following steps: (1) obtaining material A by treating an organic carbon source in the presence of an oxygen-containing atmosphere; (2) obtaining material B by activating the material A; (3) mixing the material B with a solution containing a transition metal salt, and then obtaining material C by drying; and (4) obtaining the negative electrode material by growing a heterogeneous carbon layer on the material C. The application also provides a negative electrode material and a sodium ion battery obtained by using the above preparation method. The preparation process of the negative electrode material uses an organic carbon source as a raw material, the pore size distribution of the organic carbon source is accurately shaped by controlling an activation process, and the porous carbon structure is coated with a composite carbon structure in the presence of a transition metal salt, so that the overall electrochemical performance of the negative electrode material is improved.

The embodiment of the invention relates to a seismic imaging profile resolution improving method and device, and the method comprises the steps: collecting and analyzing a plurality of seismic imaging profiles, and constructing a plurality of seismic velocity models; obtaining a first target seismic imaging profile based on the seismic convolution model and the plurality of seismic velocity models; by randomly setting different migration imaging parameters, carrying out migration imaging on the plurality of seismic velocity models by using a reverse time migration imaging method to obtain a second target seismic imaging profile; expanding a seismic imaging profile with diversity characteristics by using adaptive synthesis sampling, and adding seismic noise to the seismic imaging profile; designing an initial generative adversarial network in combination with the generative adversarial network, and training to obtain an initial resolution improvement model; and carrying out transfer learning on the initial resolution improvement model to obtain a target resolution improvement model. Therefore, the signal-to-noise ratio and the resolution ratio of the offset profile can be improved, the calculation efficiency is high, and no parameter dependence exists.