502 results about "Virulence" patented technology

The invention discloses a capture probe group for detecting pathogenic microorganisms and application of the capture probe group. The nucleic acid sequence of the capture probe group comprises a sequence as shown in SEQ ID NO. 1 to SEQ ID NO. 101. The capture probe group can cover and detect 119 types of bacteria, 32 types of fungi, 41 types of DNA viruses and 71 types of RNA viruses and covers common infectious disease pathogens, the kit containing the capture probe can cover and detect 88 types of drug-resistant and virulence genes, the detection performance of pathogenic microorganisms is greatly improved, and the sensitivity and specificity are improved.

The invention relates to the technical field of biology, in particular to a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention discloses a verticillium dahliae virulence gene VdPHO23like. The verticillium dahliae virulence gene VdPHO23like comprises a polynucleotide sequence for coding an amino acid sequence of SEQ ID NO: 3. According to the invention, expression of the gene is inhibited in a targeted manner through an RNA interference mediated gene silencing technology so as to weaken virulence of pathogenic bacteria, and an efficient, specific and environment-friendly comprehensive prevention and control strategy is provided for verticillium wilt of crops such as cotton.

The invention discloses a biological hazard big data analysis and monitoring early warning system, and relates to the technical field of public health safety, and an analysis subsystem in the system comprises a core logic module comprising a quality control unit, an error correction unit, an assembly unit and a box separation unit; the unit analysis module comprises a pathogen analysis unit, a resistance gene unit, a virulence evaluation unit and an evolution development unit; the flora integrated analysis module comprises a traceability analysis unit, a mutation characteristic unit, a propagation evolution unit and a transformation management and control unit; the early warning subsystem comprises a risk assessment and early warning system design unit, a dynamic research and propagation analysis unit, an assessment model construction unit, a pathogen evolution and function research unit, a toxicity and propagation risk comprehensive prediction unit, a pathogen risk monitoring network unit and an unknown pathogen and potential risk identification unit. According to the method, the biological hazard data can be comprehensively, efficiently and accurately analyzed.

The invention relates to the technical field of public health safety, and discloses a quantitative evaluation method and system for pathogen transmission risk, and the evaluation method comprises the following steps: step 1, obtaining virulence genes, reference virulence genes, environmental parameters, transmission factors and host risk factor parameters of pathogen samples; 2, performing variation detection on the virulence gene by adopting various variation detection algorithms to obtain all variation sites, and processing all variation sites to obtain effective variation sites; 3, constructing a virulence index model, inputting the variation distance into the virulence index model, and calculating the virulence index of the pathogenic bacteria sample; and 4, constructing a propagation risk assessment model, inputting the virulence index, the environmental factor, the propagation coefficient and the host susceptibility risk index into the propagation risk assessment model, and outputting a risk value by the propagation risk assessment model. According to the method, accurate prediction of the pathogen transmission risk can be realized.

The invention relates to the technical field of high-throughput targeted sequencing, in particular to a primer group for detecting important drug-resistant genes and virulence genes of multi-drug-resistant pathogenic bacteria and application of the primer group. Specifically, the kit comprises detection primers for multiple drug-resistant bacteria such as third-generation cephalosporin drug-resistant enterobacteriaceae bacteria, carbapenem drug-resistant enterobacteriaceae bacteria, carbapenem drug-resistant acinetobacter baumannii, rifampicin drug-resistant mycobacterium tuberculosis and the like. The method has the technical advantages of rapidness, high efficiency, strong targeting property, strong specificity and the like, is suitable for epidemic and difference analysis of various important drug-resistant genes and virulence genes carried by metagenome samples of various environments or disease materials and the like, evaluates the risk of related environments on the basis, and can further guide prevention and clinical treatment medication.

The invention discloses an application of phloretin in preparation of a medicine or a feed additive for treating or preventing swine envelope virus infection, the medicine is phloretin or oral liquid, granules, tablets and powder prepared from phloretin as an effective component and a solvent or an auxiliary material, and the use dosage is 2.5-10mg / kg of the body weight of a pig; or the phloretin is directly added into the feed, and the addition amount of the phloretin is 0.01-0.03% of the total weight of the pig feed. The phloretin or the composition containing the phloretin is used as a medicine or a feed additive, is high in safety and free of toxic and side effects, and has the effects of remarkably inhibiting replication of envelope viruses such as porcine epidemic diarrhea virus, porcine reproductive and respiratory syndrome virus and African swine fever virus and reducing the toxicity of the porcine envelope viruses; the defects of immune failure possibly caused by vaccination, virus variation caused by use of antiviral drugs and the like are overcome, the morbidity and mortality of the piglets infected with the swine envelope virus are effectively reduced, and the effect of preventing and treating or preventing the swine envelope virus infection diseases is achieved.

The invention discloses a lytic bacteriophage vBVpaSR40Z and application of the lytic bacteriophage vBVpaSR40Z to prevention and control of vibrio parahaemolyticus in an aquaculture environment. The preservation number of the vibrio parahaemolyticus phage vBVpaSR40Z is as follows: GDMCC No: 67440-B1, and the vibrio parahaemolyticus phage vBVpaSR40Z is The R40Z can be used for cracking multiple strains of vibrio parahaemolyticus and vibrio alginolyticus, has the capability of adapting to an aquaculture environment, and keeps relatively high activity within the range of 4-25 DEG C and pH of 4-10; no virulence gene or drug-resistant gene is detected through genome analysis, and phylogenetic and whole genome comparison shows that the similarity between R40Z and a related phage is lower than a species level threshold value, so that R40Z is a new phage species; the R40Z can obviously inhibit the growth of a host under various infection complex numbers. The R40Z has the advantages of strong splitting capacity, good environmental stability, high genome safety, unique evolutionary characteristics and the like, and is an aquatic vibriosis biological prevention and control bacteriophage with application potential.

The invention provides a lactobacillus pentosus and a preparation method and application of bacteriocin of the lactobacillus pentosus. The name of the strain is lactobacillus pentosus YCL31, and the strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on June 25, 2025, the preservation address is the 5th floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC No: 66589. The bacteriocin separated and purified from the metabolite of the bacterium can destroy the morphological structure of the vibrio parahemolyticus and inhibit the toxicity of the vibrio parahemolyticus, can also achieve the effect of remarkably inactivating the vibrio parahemolyticus in a metapenaeus ensis system, can effectively delay the spoilage of the metapenaeus ensis, and has a good application prospect as a bacteriostatic agent or preservative. Therefore, development and application of the novel bacteriocin have important reference value in related fields.

A bacteriophage for specifically lysing high-virulence capsular Klebsiella pneumoniae, pertaining to the field of microorganisms. The deposit number of the Klebsiella pneumoniae bacteriophage vB_kpnP_D39 is CCTCC NO: M 2024690. In the bacteriophage liquid formulation prepared on the basis of the bacteriophage, the working titer of the bacteriophage is greater than 1 × 109 PFU / mL. The bacteriophage has high specificity and can kill all high-virulence multidrug-resistant Klebsiella pneumoniae of K1, K2, and K57 capsular serotypes, with an adsorption efficiency of 99.60%. The bacteriophage exhibits a biofilm clearance rate of 47.4%-63.2% and a capsule clearance rate of 42.1%-60.6% against the high-virulence Klebsiella pneumoniae. The bacteriophage is expected to become a safe, non-toxic agent for the prevention, control, and treatment of high-virulence multidrug-resistant Klebsiella pneumoniae infections, or an antibiotic adjuvant.

The invention discloses a method and system for detecting respiratory tract pathogens, pathogen drug-resistant genes and pathogen virulence genes, and relates to the technical field of gene detection.The method comprises the steps that a respiratory tract sample of a patient is collected, DNA and RNA are extracted, and total nucleic acid is obtained; carrying out first-round PCR amplification on the total nucleic acid, purifying a first-round PCR product, carrying out second-round PCR amplification, and sorting the product to obtain a library building product; performing high-throughput sequencing on the library building product to obtain sequencing data, and detecting pathogens, drug-resistant genes and virulence genes; according to the method and system for detecting the respiratory tract pathogens, the pathogen drug-resistant genes and the pathogen virulence genes, by optimizing library preparation and direct library building of nucleic acid, the process is simplified, and efficiency and accuracy are improved; the balance reagent design guarantees library quality; the kit adapts to pathogen detection requirements in Yunnan and the like, provides efficient diagnosis, shortens detection time, improves treatment effect, regularly updates pathogen information, and keeps technical frontier.

The invention discloses trichoderma asperellum TR35 and application thereof in inhibition of mulberry bacterial wilt and prevention and treatment of mulberry bacterial wilt, the trichoderma asperellum TR35 is identified as Trichoderma asperellum, the trichoderma asperellum TR35 is preserved in Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC No.64886, and the preservation time is July 18, 2024. The trichoderma asperellum TR35 obtained through separation and screening has good inhibition and antagonism effects on mulberry ralstonia solanacearum, and can be used for preventing and treating mulberry bacterial wilt. The inhibition rate of the trichoderma asperellum TR35 to the mulberry ralstonia solanacearum reaches up to 98.25%, and the active fermentation liquor of the trichoderma asperellum TR35 not only can significantly inhibit the growth of the ralstonia solanacearum and reduce the forming ability of a biological membrane, but also can destroy the integrity and continuity of the biological membrane of the ralstonia solanacearum. In addition, the bacterial zone of the ralstonia solanacearum can be reduced, the athletic ability is reduced, and the expression of virulence-related genes in the ralstonia solanacearum is remarkably inhibited.

The present invention is in the field of agricultural pathogen control. Methods and compositions are described for controlling plant diseases caused by pathogens, including fungi and oomycete phytopathogens. Chemicals were screened for activity as autophagy inhibitors. The screening method used a bioluminescence resonance energy transfer (BRET)-based high-throughput screening strategy to identify compounds that inhibit fungal ATG4 cysteine protease-mediated cleavage of ATG8 for autophagosome formation. Ebselen and ebselen analogs were identified as inhibitors of fungal pathogens ATG4-mediated ATG8 processing. Identified chemicals inhibit spore germination, hyphal development, and appressorium formation. The autophagy inhibitors significantly reduced the virulence of pathogens in their corresponding plant hosts. The invention also provides for compositions comprising the same, methods of making the same, and methods of controlling plant disease.

The invention relates to the technical field of gene engineering, and particularly discloses streptococcus agalactiae WC1535 delta cps delta hylB as well as a construction method and application thereof. The streptococcus agalactiae WC1535 [delta] cps [delta] hylB is preserved in Guangdong Microbial Culture Collection Center on February 28, 2025, the preservation number is GDMCC NO.65955, and the address of the preservation unit is the 5th building, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. Compared with a traditional single-fragment deletion method, the toxicity of the deletion strain is not prone to being enhanced, and compared with a traditional injection immune vaccine, the gene deletion strain is used for preparing the tilapia oral live vaccine, the immune operation is simpler, and more labor cost is saved.

The invention discloses a construction method of a nocardia seriolae mycolic acid deficient strain, which belongs to the technical field of aquaculture prevention and control, and comprises the following steps: strain and material preparation, culture and genome extraction, upstream and downstream fragment cloning to construct deletion plasmids, competent cell preparation, electrotransformation and screening, and biological characteristic and toxicity analysis. The nocardia seriola seriola mycolic acid defect type strain is constructed and compared with a wild strain and a back-filling strain for research, the effect of the mycolic acid in the aspects of growth, form, toxicity and the like of thalli can be defined, the relationship between the mycolic acid and the pathogenicity of the thalli can be deeply known, and an important clue is provided for revealing the pathogenic mechanism of the nocardia seriola seriola.

The present invention relates to recombinant virulence attenuated Gram-negative bacterial strains and its use in a method of treating cancer in a subject.

The antibody disclosed by the invention can specifically target high-virulence klebsiella pneumoniae of different serotypes, and specifically targets klebsiella pneumoniae of K1 and K2 serotypes. The antibody disclosed by the invention can be combined with high-toxicity klebsiella pneumoniae extracellular capsular polysaccharide with high affinity, has very strong neutralizing activity on klebsiella pneumoniae, and can mediate complement and mature neutrophil to neutralize and kill bacteria in an in-vitro killing experiment, so that the bacterial density is reduced, and the killing effect is improved. The traditional Chinese medicine composition can effectively prevent and treat high-toxicity klebsiella pneumoniae clinical infection and effectively improve pulmonary infection, provides a way for solving the problems of klebsiella pneumoniae infection and multiple drug resistance, has remarkable significance in preventing, diagnosing and treating klebsiella pneumoniae infection, and has important value in development of a new generation of klebsiella pneumoniae medicine.

The present invention belongs to the field of biotechnology, and specifically relates to the use of the CD630_27900 gene in reducing the tolerance of Clostridium difficile. Knocking out the CD630_27900 gene can reduce the tolerance of Clostridium difficile to acidic environments and antibiotics and reduce its cytotoxicity; the sequence of the CD630_27900 gene is shown in SEQ ID NO.1; the antibiotics include ampicillin, metronidazole, amoxicillin, vancomycin, norfloxacin, cefoxitin, clindamycin and kanamycin. The research results of the present invention suggest that the CD630_27900 gene can be used as a target for inhibiting the drug resistance and reducing the toxicity of Clostridium difficile, providing a new idea for the treatment of Clostridium difficile infection (CDI).

The invention belongs to the technical field of biological control, and relates to a preparation method of prodenia litura nuclear polyhedrosis virus concentrate, which comprises the following steps: cultivating and hatching prodenia litura eggs at constant temperature, culturing the prodenia litura eggs in a dark place into 4-day-old larvae, performing virus infection and proliferation on the 4-day-old larvae, collecting and screening dead insects with strong toxicity and early death as virus semi-finished products, and refrigerating and storing the virus semi-finished products. And finally, preparing the virus semi-finished product into mother powder to obtain the prodenia litura nuclear polyhedrosis virus mother drug. The method comprises the following steps: extracting a parent drug sample to obtain a virus polyhedrosis, extracting a DNA comparison gene sequence of the virus polyhedrosis, prodenia litura nuclear polyhedrosis virus which is identified as prodenia litura nuclear polyhedrosis virus when the similarity between the virus polyhedrosis and Spodoptera litura Nucleoplydrovirus reaches 80% or above, and measuring various performance indexes, so that the prodenia litura nuclear polyhedrosis virus can be identified as prodenia litura nuclear polyhedrosis virus. The prodenia litura nuclear polyhedrosis virus mother drug prepared by the preparation method is used for preventing and treating lepidoptera pests such as prodenia litura, has important significance, has good development and application prospects, and has application value in preparation of insecticides and prevention and treatment of flat thorn moths.

Disclosed are antibodies to anti-hepatitis B surface antigen (HBsAg) (especially humanized antibodies), nucleic acid molecules encoding same, methods for preparing same, and pharmaceutical compositions containing same. The antibodies have higher affinity for HBsAg at neutral pH than at acidic pH, thereby significantly enhancing the virus clearance efficiency and prolonging the virus inhibition time. The antibodies and the pharmaceutical compositions can be used for preventing and / or treating HBV infections or diseases related to HBV infections (e.g., hepatitis B), for neutralizing the virulence of HBV in a subject (e.g., a human), for reducing the serum level of HBV DNA and / or HBsAg in the body of the subject, or for activating the humoral immune response of the subject (e.g., a chronic HBV infected or chronic hepatitis B patient) against HBV.

The invention provides application of a bacillus subtilis strain Rhs-L31 in prevention and treatment of pine wood nematode disease, and relates to an agricultural environment microbial technology. In particular relates to a bacillus friaricola strain Rhs-L31 which is separated from rhizosphere soil of Chinese pines and application of the bacillus friaricola strain Rhs-L31 in prevention and treatment of pine wood nematode diseases. The strain is preserved in the China Center for Type Culture Collection in Wuhan University on December 25, 2024, and the preservation number is CCTCC (China Center for Type Culture Collection) 20242847. The in-vitro poisoning activity of the strain Rhs-L31 on pine wood nematodes can reach 100% within 24 hours; a fermentation filtrate and a zymophyte suspension of the strain Rhs-L31 have a remarkable inhibition effect on the propagation of the pine wood nematodes; the prevention and treatment effect of a zymophyte suspension of the strain Rhs-L31 on the pine wood nematode disease of the Chinese pine potted seedlings can reach 50%-72.5%; the bacillus subtilis strain Rhs-L31 disclosed by the invention has a good application prospect in the field of pollution-free prevention and treatment of pine wood nematode diseases.

The invention relates to the technical field of viruses, in particular to a construction method and application of an African swine fever virus strain. According to the invention, the three virulence genes MGF110-9L, EP402R and I73R of the African swine fever virus are taken as targets for the first time to construct a virus strain with deletion of the three virulence genes, and the virus strain is found to be capable of effectively reducing the virus toxicity but retaining better immunogenicity, can improve the immunity of an organism to the African swine fever virus, and has a relatively great application prospect.

The invention discloses a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention relates to the technical field of biology, and provides a method for preventing and treating cotton verticillium wilt based on RNAi (RNA interference) as well as related components and application thereof. Specifically, the invention discloses application of the verticillium wilt prevention and treatment by a method for inhibiting or silencing the expression of verticillium dahliae pathogenic gene VdHDAC (coding protein of the verticillium dahliae pathogenic gene VdHDAC is shown as SEQ ID NO: 3), the core of the verticillium wihliae pathogenic gene VdHDAC is to provide dsRNA molecules capable of targeting the gene, and the positive-sense strand of the dsRNA molecules is preferably selected from SEQ ID NO: 5, SEQ ID NO: 8, SEQ ID NO: 9 or SEQ ID NO: 11. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

The invention belongs to the technical field of medicines, and provides a method for screening a mutant strain and a high-virulence Seneca virus A mutant strain. The method for promoting Senecavirus A mutation provided by the invention comprises the following steps: inserting an RNAi target sequence between 3Dpol and a 3'untranslated region of an SVA starting strain; the target sequence of the RNAi contains a complementary sequence of an endogenous sequence of the miRNA. According to the research, mutagenesis is carried out on SVA by constructing a high RNAi pressure system, and a method for screening the mutant strain SVA is carried out by simulating an antiviral RNAi mechanism in a host, so that a natural evolution process of the virus is better met, a large number of stably inherited mutant strains can be obtained, and an efficient research and development means is provided for vaccine research and development aiming at the virus.

The present disclosure relates generally to conjugated plasmids comprising a multiple entry exclusion system derived from a plasmid carrying a gene conferring undesired traits of interest, such as pathogenic traits, such as antimicrobial drug resistance (AMR), virulence determinants, metal resistance, or other undesired traits, such as, for example, multiple entry exclusion systems derived from plasmids carrying a gene conferring undesired traits of interest. And the use of those probiotic plasmids for protecting bacterial populations (e.g., present in the gut, present in the environment, etc.) from obtaining plasmids imparting the undesirable traits and thereby preventing the propagation of the undesirable traits (e.g., AMR, virulence, metal resistance, etc.) within microbiota.

The invention discloses a salmonella bacteriophage cocktail and application thereof, and belongs to the technical field of bioengineering, the salmonella bacteriophage cocktail is characterized by comprising a salmonella bacteriophage vBSenMP9, a salmonella bacteriophage vBSalSPS87 and a salmonella bacteriophage vBSalSPMY153, and the salmonella bacteriophage vBSalSPMY153 is prepared from salmonella bacteriophage vBSenMP9, salmonella bacteriophage The salmonella bacteriophage vBSenMP9 is preserved in the China Center for Type Culture Collection, and the preservation number of the salmonella bacteriophage vBSenMP9 is CCTCC M 20231900; the salmonella bacteriophage vBSalSPS87 is preserved in the China Center for Type Culture Collection, and the preservation number of the salmonella bacteriophage vBSalSPS87 is CCTCC M 20242948; the salmonella bacteriophage vBSalSPMY153 is preserved in the China Center for Type Culture Collection, and the preservation number of the salmonella bacteriophage vBSalSPMY153 is CCTCC M 20242949. The salmonella bacteriophage cocktail has a wide splitting spectrum for salmonella, and genes related to lyogen, drug resistance and toxicity are not found in each salmonella bacteriophage genome, so that the salmonella bacteriophage cocktail is safe when being applied to research and development of medicines and sterilization products.

The invention relates to the technical field of molecular biological detection, and particularly provides a reagent for respiratory tract infection pathogen identification and drug resistance and virulence gene detection. Each primer in the plurality of primers comprises a first sequence as shown in SEQ ID NO: 1-165 and an optional second sequence for distinguishing sample sources. Target spots detected by the super-multiplex primer combination comprehensively cover pathogen directories (totally 353 types) of latest respiratory tract infection diagnosis guidelines at home and abroad, low-cost, high-sensitivity, high-specificity and high-flux detection is achieved, and clinical medication can be effectively and comprehensively guided.

The invention relates to the technical field of animal virology, in particular to a target sequence for detecting the virulence of a largemouth bass frog iridovirus and application of the target sequence. The invention provides a target sequence for detecting the virulence of a largemouth bass frog iridovirus. The nucleotide sequence of the target sequence is as shown in SEQ ID NO: 1; the invention also provides a primer pair for detecting the virulence of the largemouth bass frog iridovirus. The detection method provided by the invention can efficiently and accurately distinguish the virulent strain and the virulent strain of the largemouth bass frog iridovirus, the detection time can be completed within 1.5 hours, a powerful support is provided for accurate judgment of the virulence of the largemouth bass frog iridovirus, and an effective means is also provided for screening the virulent strain and the virulent strain of the largemouth bass frog iridovirus.

The invention discloses a bacillus thuringiensis CMS-BT17, which is classified and named as bacillus thuringiensis, is preserved in the China General Microbiological Culture Collection Center (CGMCC), has the preservation number of CGMCC 35313, and is preserved on July 21, 2025. The bacillus thuringiensis strain CMS-BT17 directly separated and screened from a zizania aquatica soil sample in Haikou City of Hainan carries four insecticidal protein coding genes of Cry8Ca, Cry8Da, Spp1Aa and Vpb4Ca, not only is high in insecticidal toxicity and good in insecticidal effect, but also has a certain effect of inhibiting tomato fungal diseases, can be widely applied to prevention and control of agricultural diseases and pests, and has a wide application prospect. The sustainable development of green agricultural production is promoted.

The invention provides a novel bovine nodular skin disease virus LSDV-125 strain and application thereof in vaccine preparation, and belongs to the technical field of virology. The problem that a novel vaccine strain which is clear in genetic background, stably weakened in virulence and capable of inducing broad-spectrum immune response is urgently needed is solved. The invention relates to a sheep pox virus strain, in particular to a sheep pox virus LSDV-125 which is named sheep pox virus LSDV-HLJ-2023, has the preservation number of CCTCC (China Center for Type Culture Collection) NO: V202537, and is preserved in China Center for Type Culture Collection on May 13, 2025. The method is mainly used for developing efficient and safe attenuated live vaccines.

The invention relates to the field of biological control, and discloses screening of entomogenous fungi with high pathogenicity to prodenia litura and compound application of the entomogenous fungi and emamectin benzoate. The Penicillium Pomelis strain with high toxicity to prodenia litura is collected from Xinjiang Checkoean cotton land. The invention further provides an efficient, pollution-free and residue-free insecticide combined with emamectin benzoate and Penicillium bauhiniae, which has a synergistic interaction effect and is prepared by combining emamectin benzoate and Penicillium bauhiniae. Research on the compounding effect of emamectin benzoate and Penicillium Polish shows that after compounding, the insecticide can show an obvious synergistic effect, the prevention and treatment effect is improved, the use dosage of a single chemical pesticide can be obviously reduced, the environmental pollution is reduced, and the problems that the existing pesticide is easy to generate resistance, the pesticide effect is not obvious and the like are effectively solved.