11 results about "Systemic immunity" patented technology

The invention discloses a mannose modified liposome gel microsphere oral delivery system based on cations and application of the mannose modified liposome gel microsphere oral delivery system. The oral delivery system comprises a core layer and a wrapping layer, the core layer is mannose modified cationic liposome loaded with a mucous membrane adjuvant retinoic acid and an antigen, and the wrapping layer is sodium alginate gel vulcanized and modified by cysteine. The mannose modified cationic liposome is prepared by coupling mannose on the surface of the cationic liposome, and the cysteine modified sodium alginate gel is used for protecting antigens in the core layer from being eroded by gastric juice, enhancing the adhesiveness of a delivery system in the intestinal tract and prolonging the retention time of the delivery system in the intestinal tract; in addition, the R-MLip can be expanded and released under the environment that the pH of the intestinal tract is increased. The inside of the gel microsphere of the oral delivery system shows a regular net-shaped structure and has abundant holes and channels, and the oral delivery system shows a good prospect in the aspect of enhancing mucous membrane and systemic immunity and possibly becomes a potential substitute of a traditional attenuated live vaccine in the future.

The present application relates to the technical field of veterinary biological products, in particular to application of oridonin in preparation of poultry vaccine adjuvant. The poultry vaccine adjuvant is composed of oridonin, solvent and buffer. Experiments prove that after mixing the poultry vaccine adjuvant with poultry inactivated vaccine (such as Newcastle disease vaccine) for immunization, the humoral immune level of poultry can be significantly improved (HI titer of 21d after the second immunization is increased by more than 1.5 log2), and the secretion level of specific IgY (systemic immunity) and IgA (mucosal immunity) in serum can be extremely significantly up-regulated, and the high level of antibody is maintained for more than 4 weeks longer than that of the conventional vaccine group. Compared with commercial adjuvant ISA 201, the present application has better immune enhancement activity, high safety, no adverse reaction at injection site, and wide clinical application prospect.

The invention discloses a compound preparation for treating osteoarthritis and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine preparations. Comprising the following raw materials in parts by weight: 15-19 parts of wine-processed radix achyranthis bidentatae, 13-15 parts of salted eucommia ulmoides, 11-13 parts of angelica sinensis, 9-11 parts of bran-fried rhizoma atractylodis, 11-13 parts of semen coicis, 5-7 parts of poria cocos, 4-6 parts of wine-processed flos carthami, 4-6 parts of vinegar-processed rhizoma corydalis, 4-6 parts of radix paeoniae alba, 8-10 parts of sand-scalded rhizoma drynariae and 4-6 parts of honey-fried liquorice. According to the compound preparation for treating osteoarthritis and the preparation method, arthralgia and stiffness can be rapidly relieved, long-acting repair and adjustment can be achieved, intestinal flora and systemic immunity are synergistically adjusted through targeted colon, and the pathological process of osteoarthritis is blocked from multiple ways. Clinical verifications show that the curative effect of the traditional Chinese medicine composition is obviously superior to that of conventional preparations and first-line western medicines, the safety is better, and an innovative treatment choice which takes effect quickly, takes effect for a long time and treats both symptoms and root causes is provided for patients.

The application specifically discloses a kind of nanovaccine for thrombosis prevention and its preparation method and application, it is related to thrombotic disease, especially related to the nanovaccine for treating thrombotic disease.The nanovaccine provided by the application has good immunogenicity without macromolecular heterologous protein carrier such as KLH;The process simplicity and repeatability are improved by reducing complex chemical coupling and purification steps;The dependence on traditional high-stimulating immune adjuvant is reduced, and the biological safety is improved;The stability of antigen in gastrointestinal environment is significantly improved;Active targeting delivery of M cells and dendritic cells is realized;Dendritic cell maturation and antigen presentation are promoted, and mucosal immunity and systemic immunity level are improved;The advantages such as effectively inhibiting thrombosis are realized, and a new type of oral antithrombotic vaccine system with high compliance suitable for long-term thrombosis prevention is constructed.

The invention discloses an application of cell-penetrating peptide modified virus nanoparticles in preparation of a product for cryoablation treatment. The cell-penetrating peptide modified virus nanoparticles have autoimmunogenicity and ice crystal nucleation effect, have high biological safety, can be used for preparing cryoablation treatment products, especially in cryoablation cancer therapy, can improve the killing effect on tumors and improve the whole-body immune effect, and can be used for preparing cryoablation treatment products. The tumor recurrence and metastasis are effectively delayed.

The present invention concerns isolated human breast milk exosomes, and compositions thereof, and uses of said exosomes and compositions for promoting systemic immunity and treating and / or preventing systemic inflammation.

The invention discloses a micro-ecological fermented feed suitable for piglets and a preparation method of the micro-ecological fermented feed, and belongs to the technical field of functional feeds. The preparation method comprises the following steps: 1) preparing functional hydrolysate rich in arginine and branched chain amino acid from plant raw materials through composite enzymolysis; (2) inoculating a functional bacterial agent consisting of lactobacillus plantarum with high arginase activity, nitrate reductase positive bacillus subtilis and clostridium butyricum; (3) accurately controlling the fermentation process by taking the molar ratio of arginine to citrulline being 0.8-1.5 as a fermentation end point, directionally enriching nitric oxide precursors such as citrulline in the feed, and constructing an endogenous NO slow-release system; the product can safely and efficiently activate NO metabolism of intestinal tracts of piglets, synergistically improve intestinal barrier, flora balance, digestive function and whole body immunity, significantly increase daily gain, reduce diarrhea rate, replace zinc oxide and antibiotics, and realize green and healthy breeding.

The invention discloses a composition, which comprises engineering bacteria for expressing TLS induced protein and mesenchymal stem cells, and can effectively induce TLS formation, inhibit tumor growth, improve systemic immunity, inhibit distant metastasis and prevent tumor recurrence in tumor tissues, thereby providing a new technical path for enhancing the universality and accessibility of immunotherapy. And an innovative platform is provided for the research on a combined treatment scheme of tumor immunotherapy.

The present invention relates to a biopharmaceutical material and application of co-expressed porcine interleukin 15, 21 and 23, and relates to the field of biotechnology. The present invention provides a fusion protein comprising porcine interleukin 15, porcine interleukin 21 and porcine interleukin 23, consisting of an amino acid sequence as shown in SEQ ID NO: 1. A nucleic acid molecule encoding the fusion protein. A biopharmaceutical material comprising an expression cassette, a recombinant vector, a transfected cell, a recombinant microorganism, a culture product of the transgenic cell line or a fermentation product of the recombinant microorganism having the nucleic acid molecule. The present invention obtains a fusion protein by genetic recombination and fusing porcine interleukin 15, 21 and 23 genes, connecting them to a eukaryotic expression platform, and finding that the fusedly expressed IL-15, 21 and 23 have good biological effects of significantly enhancing the mucosal immune barrier function of animals, enhancing their systemic immunity level and anti-bacterial infection ability, and promoting the growth and development of animals.

The invention discloses a preparation method and application of oral microspheres containing chlorella and ginseng polysaccharide, and belongs to the technical field of biological medicine. The oral microspheres are prepared in a water-in-oil manner by utilizing a microfluidic technology, the microspheres are loaded with chlorella and ginseng polysaccharide, and a thermal response gel network is formed by taking methylacryloylated gelatin as a carrier. The prepared microspheres are stable in the stomach environment, can be gelated and gradually degraded under the induction of temperature in the intestinal tract after being orally taken, and slowly release active ingredients, so that the intestinal targeted delivery efficiency of the microspheres is effectively improved. The oral microspheres have multiple functions of regulating intestinal flora, repairing intestinal barriers, reducing intestinal permeability and regulating system immunity, and can remarkably improve intestinal and joint pathological manifestations related to rheumatoid arthritis. Furthermore, the joint cavity injection glucocorticoid is combined, so that the synergistic treatment of intestinal tracts and joints can be realized, and the anti-inflammatory and tissue protection effects are enhanced. The system is good in safety and stable in preparation process, and has wide application potential in rheumatoid arthritis and other chronic inflammatory diseases.

The invention discloses lactobacillus reuteri for promoting host beta defensin and immune globulin secretion and relieving vaginal mucosa damage through oral administration and external application and a metagen thereof, and belongs to the technical field of microorganisms. The lactobacillus reuteri is separated from vaginal secretion of healthy women, has the effects of improving vaginal mucosa injury and inhibiting vagina inflammation, and is specifically reflected in that secretion of a vaginal epithelial cell host defense peptide HBD2 is promoted; the secretion of a vaginal epithelial cell host polymeric immunoglobulin receptor pIgR is promoted; the secretion of animal vaginal tissue host defense peptide mBD3 is improved; the production of secretory immunoglobulin sIgA is promoted; the generation of animal serum immune globulin IgG is promoted, so that the systemic immunity is improved; the pathological characterization of the vagina of the mouse is improved. Therefore, the lactobacillus reuteri has a huge application prospect in products for enhancing the anti-infection barrier of the host and improving the local immunity and systemic immunity of the host.