7 results about "Systemic immunity" patented technology

The invention discloses a mannose modified liposome gel microsphere oral delivery system based on cations and application of the mannose modified liposome gel microsphere oral delivery system. The oral delivery system comprises a core layer and a wrapping layer, the core layer is mannose modified cationic liposome loaded with a mucous membrane adjuvant retinoic acid and an antigen, and the wrapping layer is sodium alginate gel vulcanized and modified by cysteine. The mannose modified cationic liposome is prepared by coupling mannose on the surface of the cationic liposome, and the cysteine modified sodium alginate gel is used for protecting antigens in the core layer from being eroded by gastric juice, enhancing the adhesiveness of a delivery system in the intestinal tract and prolonging the retention time of the delivery system in the intestinal tract; in addition, the R-MLip can be expanded and released under the environment that the pH of the intestinal tract is increased. The inside of the gel microsphere of the oral delivery system shows a regular net-shaped structure and has abundant holes and channels, and the oral delivery system shows a good prospect in the aspect of enhancing mucous membrane and systemic immunity and possibly becomes a potential substitute of a traditional attenuated live vaccine in the future.

The present application relates to the technical field of veterinary biological products, in particular to application of oridonin in preparation of poultry vaccine adjuvant. The poultry vaccine adjuvant is composed of oridonin, solvent and buffer. Experiments prove that after mixing the poultry vaccine adjuvant with poultry inactivated vaccine (such as Newcastle disease vaccine) for immunization, the humoral immune level of poultry can be significantly improved (HI titer of 21d after the second immunization is increased by more than 1.5 log2), and the secretion level of specific IgY (systemic immunity) and IgA (mucosal immunity) in serum can be extremely significantly up-regulated, and the high level of antibody is maintained for more than 4 weeks longer than that of the conventional vaccine group. Compared with commercial adjuvant ISA 201, the present application has better immune enhancement activity, high safety, no adverse reaction at injection site, and wide clinical application prospect.

The invention discloses a compound preparation for treating osteoarthritis and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine preparations. Comprising the following raw materials in parts by weight: 15-19 parts of wine-processed radix achyranthis bidentatae, 13-15 parts of salted eucommia ulmoides, 11-13 parts of angelica sinensis, 9-11 parts of bran-fried rhizoma atractylodis, 11-13 parts of semen coicis, 5-7 parts of poria cocos, 4-6 parts of wine-processed flos carthami, 4-6 parts of vinegar-processed rhizoma corydalis, 4-6 parts of radix paeoniae alba, 8-10 parts of sand-scalded rhizoma drynariae and 4-6 parts of honey-fried liquorice. According to the compound preparation for treating osteoarthritis and the preparation method, arthralgia and stiffness can be rapidly relieved, long-acting repair and adjustment can be achieved, intestinal flora and systemic immunity are synergistically adjusted through targeted colon, and the pathological process of osteoarthritis is blocked from multiple ways. Clinical verifications show that the curative effect of the traditional Chinese medicine composition is obviously superior to that of conventional preparations and first-line western medicines, the safety is better, and an innovative treatment choice which takes effect quickly, takes effect for a long time and treats both symptoms and root causes is provided for patients.

The application specifically discloses a kind of nanovaccine for thrombosis prevention and its preparation method and application, it is related to thrombotic disease, especially related to the nanovaccine for treating thrombotic disease.The nanovaccine provided by the application has good immunogenicity without macromolecular heterologous protein carrier such as KLH;The process simplicity and repeatability are improved by reducing complex chemical coupling and purification steps;The dependence on traditional high-stimulating immune adjuvant is reduced, and the biological safety is improved;The stability of antigen in gastrointestinal environment is significantly improved;Active targeting delivery of M cells and dendritic cells is realized;Dendritic cell maturation and antigen presentation are promoted, and mucosal immunity and systemic immunity level are improved;The advantages such as effectively inhibiting thrombosis are realized, and a new type of oral antithrombotic vaccine system with high compliance suitable for long-term thrombosis prevention is constructed.

The invention discloses a micro-ecological fermented feed suitable for piglets and a preparation method of the micro-ecological fermented feed, and belongs to the technical field of functional feeds. The preparation method comprises the following steps: 1) preparing functional hydrolysate rich in arginine and branched chain amino acid from plant raw materials through composite enzymolysis; (2) inoculating a functional bacterial agent consisting of lactobacillus plantarum with high arginase activity, nitrate reductase positive bacillus subtilis and clostridium butyricum; (3) accurately controlling the fermentation process by taking the molar ratio of arginine to citrulline being 0.8-1.5 as a fermentation end point, directionally enriching nitric oxide precursors such as citrulline in the feed, and constructing an endogenous NO slow-release system; the product can safely and efficiently activate NO metabolism of intestinal tracts of piglets, synergistically improve intestinal barrier, flora balance, digestive function and whole body immunity, significantly increase daily gain, reduce diarrhea rate, replace zinc oxide and antibiotics, and realize green and healthy breeding.

The invention discloses a composition, which comprises engineering bacteria for expressing TLS induced protein and mesenchymal stem cells, and can effectively induce TLS formation, inhibit tumor growth, improve systemic immunity, inhibit distant metastasis and prevent tumor recurrence in tumor tissues, thereby providing a new technical path for enhancing the universality and accessibility of immunotherapy. And an innovative platform is provided for the research on a combined treatment scheme of tumor immunotherapy.

The invention discloses a preparation method and application of oral microspheres containing chlorella and ginseng polysaccharide, and belongs to the technical field of biological medicine. The oral microspheres are prepared in a water-in-oil manner by utilizing a microfluidic technology, the microspheres are loaded with chlorella and ginseng polysaccharide, and a thermal response gel network is formed by taking methylacryloylated gelatin as a carrier. The prepared microspheres are stable in the stomach environment, can be gelated and gradually degraded under the induction of temperature in the intestinal tract after being orally taken, and slowly release active ingredients, so that the intestinal targeted delivery efficiency of the microspheres is effectively improved. The oral microspheres have multiple functions of regulating intestinal flora, repairing intestinal barriers, reducing intestinal permeability and regulating system immunity, and can remarkably improve intestinal and joint pathological manifestations related to rheumatoid arthritis. Furthermore, the joint cavity injection glucocorticoid is combined, so that the synergistic treatment of intestinal tracts and joints can be realized, and the anti-inflammatory and tissue protection effects are enhanced. The system is good in safety and stable in preparation process, and has wide application potential in rheumatoid arthritis and other chronic inflammatory diseases.