67 results about "Immune barrier" patented technology

The invention belongs to the technical field of biological medicines, and particularly relates to a plant lactobacillus YYY01 strain and application thereof in anti-inflammation and production of gamma-aminobutyric acid. The preservation number of the plant lactobacillus YYY01 provided by the invention is GDMCC (China General Microbiological Culture Collection Center) NO: 65147. The plant lactobacillus YYY01 has relatively good biological safety, relatively good survival in a gastrointestinal tract environment and certain intestinal tract adhesion capacity; the plant lactobacillus YYY01 can produce gamma-aminobutyric acid at high yield, and has the effects of resisting inflammation, regulating intestinal flora and enhancing immune barrier. Good application prospects are shown in the aspects of improving the yield of gamma-aminobutyric acid and preparing products containing gamma-aminobutyric acid.

The invention relates to the technical field of microorganisms, in particular to a bifidobacterium animalis subsp. Lactis and application thereof, and particularly provides a bifidobacterium animalis subsp. Lactis BX-BC08 which is beneficial for restoring the microecological balance of the intestinal tract and enhancing the immune barrier function of the intestinal tract. Experiments prove that the bifidobacterium animalis subsp. Lactis BX-BC08 disclosed by the invention can regulate an immune system and relieve excessive immune response, can reduce generation of inflammatory factors and relieve skin inflammation symptoms for autoimmune related diseases such as atopic dermatitis, is relatively good in tolerance to a human body, and greatly reduces adverse reaction of the human body.

The invention belongs to the technical field of biological medicine, and particularly relates to application of garlic polysaccharide in preparation of a product for repairing intestinal injury caused by constipation. An embodiment verifies that the garlic polysaccharide can effectively repair intestinal physical, chemical and immune barrier injuries caused by constipation, and by up-regulating expression of tight junction proteins (such as ZO-1 and Occludin) and reducing expression of Claudin-1, the garlic polysaccharide can enhance tight junction between intestinal epithelial cells and reduce intestinal permeability; besides, the garlic polysaccharide can promote secretion of intestinal mucoprotein and enhance the protection function of the intestinal mucous layer by up-regulating expression of MUC2 and MUC4 genes. The invention discloses a multi-aspect action mechanism of the garlic polysaccharide in repairing intestinal injury caused by constipation, and the garlic polysaccharide is expected to become a novel intestinal repairing agent for treating constipation and related complications thereof.

The present invention belongs to the field of biomedicine technology, and specifically relates to a strain of Lactobacillus plantarum YYY01 and its application in anti-inflammatory and production of γ-aminobutyric acid. The Lactobacillus plantarum YYY01 provided by the present invention is deposited with GDMCC NO: 65147. The Lactobacillus plantarum YYY01 has good biosafety, survives well in the gastrointestinal environment and has a certain intestinal adhesion ability; Lactobacillus plantarum YYY01 can produce high γ-aminobutyric acid, has anti-inflammatory, intestinal flora regulation and immune barrier enhancement effects. It shows good application prospects in increasing the yield of γ-aminobutyric acid and preparing products containing γ-aminobutyric acid.

The invention belongs to the technical field of biological medicines, and particularly relates to application of extracellular vesicle-like particles derived from divaricate saposhnikovia roots in preparation of medicines for preventing and treating intestinal diseases. Research on the physiological efficacy of the extracellular vesicle-like particles from divaricate saposhnikovia root shows that the extracellular vesicle-like particles can relieve colon shortening and colon tissue damage caused by dextran sodium sulfate, relieve intestinal inflammation and promote repair of intestinal barriers including mechanical barriers, chemical barriers and immune barriers. The polypeptide has potential for preparing the medicine for treating the intestinal diseases, and a new strategy is provided for preventing or treating the intestinal diseases.

The invention discloses preparation and application of aquatic plant extracellular vesicles with an intestinal barrier regulating function, and belongs to the field of bioengineering technology and health food. The method mainly comprises the following steps: efficiently extracting aquatic plant extracellular vesicles by utilizing a tangential phase flow technology, and researching the functional activity of the aquatic plant extracellular vesicles. The obtained extracellular vesicles have good intestinal stability and biocompatibility, and also have significant antioxidant activity and anti-inflammatory effect. In a carbon tetrachloride induced chronic hepatic fibrosis model, the extracellular vesicles can reduce LPS (Lipopolysaccharide) generated by excessive reproduction of intestinal microorganisms from entering the liver by regulating intestinal tight junction protein, so that the liver inflammation is relieved. In addition, the extracellular vesicles can also regulate intestinal flora and immune barriers, and inflammatory response caused by chronic hepatic fibrosis is relieved through the intestinal liver axis. The extracellular vesicles prepared by the method have wide application prospects and economic benefits in anti-inflammatory drugs, nano-drug carriers and functional skin care products.

The invention provides a composition for improving a barrier function and application thereof, and relates to the technical field of cell injury nursing. Gamma-aminobutyric acid (GABA) and cordyceps militaris fermentation filtrate (FCM) are used for treating barrier damage for the first time. Specifically, the combination of the gamma-aminobutyric acid and the cordyceps militaris fermentation filtrate can significantly improve damage of an epithelial mechanical barrier and an immune barrier caused by external stimulation, so that the epithelial barrier function can be prevented and improved from multiple dimensions.

The invention discloses a plant fermentation composition for enhancing scalp immune barrier and melanocyte activity and a preparation method and application of the plant fermentation composition, and belongs to the technical field of microbial fermentation and skin immunization. Performing fermentation by utilizing Wickerhamomyces anomalus and saccharomyces cerevisiae; the mass ratio of the Wickerhamomyces anomalus to the saccharomyces cerevisiae is (3-10): 1; the weight ratio of the radix polygoni multiflori to the radix asparagi to the black tea is (10-30): (1-10): 1. The invention provides a cosmetic raw material for promoting melanogenesis by enhancing innate immune response of melanocytes by utilizing a microbial fermentation technology, and solves the technical problem that black hair becomes white. The cosmetic provided by the invention can improve the health of hair and scalp, has a good hair blackening effect, and effectively prevents the hair from becoming grey and white.

The invention provides application of lactobacillus rhamnosus GOLDGUT-M520 or a composition of the lactobacillus rhamnosus GOLDGUT-M520 in preparation of a product for enhancing the immunity of an organism. The lactobacillus rhamnosus GOLDGUT-M520 has the function of enhancing the immunity in both a viable form and an inactivated form of the lactobacillus rhamnosus GOLDGUT-M520. The lactobacillus rhamnosus GOLDGUT-M520 has a good antibacterial capability on escherichia coli and staphylococcus aureus which are pathogenic to intestinal tracts, and is beneficial to enhancing a chemical barrier in an innate immune barrier; the strain can improve the phagocytic activity of macrophages and enhance the innate immune response; the strain can improve the T cell proportion, the B cell proportion and the immune globulin level in mesenteric lymph nodes of immunocompromised mice, and can enhance adaptive immune response. The lactobacillus rhamnosus GOLDGUT-M520 can comprehensively play a role in enhancing immunity, and meanwhile, the inherent immune barrier, the inherent immune response and the adaptive immune response are enhanced.

The invention provides a composition for promoting differentiation of intestinal immune cells as well as a preparation method and application of the composition. According to the composition, 1, 3-dioleic acid-2-palmitic acid triglyceride and breast milk oligosaccharide are used in a combined manner, so that monocytes can be effectively promoted to differentiate into intestinal macrophages, and inflammation is reduced to a certain extent. After the monocytes are differentiated into the intestinal macrophages, the intestinal immune homeostasis and intestinal microbiota inflammatory response can be regulated, so that the mucosal immune homeostasis and intestinal epithelial cell barrier function are maintained. Therefore, the composition provided by the invention can improve the intestinal immune barrier by promoting the monocytes to differentiate into the intestinal macrophages, and provides a new thought for improving the early intestinal barrier of infants through nutritional supplement.

The application discloses an active oxygen response type bacteriostatic and repair promoting hydrogel and a preparation method and application thereof, the hydrogel is formed by disulfide bond cross-linked hydrogel in response to active oxygen free radicals in the vagina of vaginitis patients through thiol modified hyaluronic acid (HASH), and can enhance in-vivo retention by thiol and vaginal tissue interaction. Cationic surfactant such as didecyldimethylammonium chloride (DDAC) as the bacteriostatic component of the hydrogel, efficiently killing pathogenic bacteria while having good biocompatibility, and is not prone to cause bacterial drug resistance. As a natural immune regulator, beta-glucan regulates the vaginal microenvironment, promotes the polarization of macrophages into anti-inflammatory M2 type and promotes cell migration, etc., to promote the repair of the vaginal immune barrier, regulate the vaginal flora, and reduce the probability of vaginitis recurrence. The HASH hydrogel provides an efficient, convenient and highly transformative potential strategy to provide more choices for the treatment of vaginitis.

The invention provides an active therapeutic coating formed on a mucous membrane interface in situ and a preparation method and application thereof, and belongs to the technical field of biological medicine. The invention provides a coating composition for treating mucosal inflammatory diseases. The coating composition comprises probiotics, trivalent bismuth and ethyl gallate. The coating composition can be prepared into active therapeutic coatings (LSTC) formed on different mucous membrane surfaces in situ, and the LSTC has the effect of synergistically regulating a physicochemical barrier, a microbial barrier and an immune barrier. The LSTC disclosed by the invention has a relatively good treatment effect on mucosal inflammation related diseases.

The present invention discloses a plant fermentation composition for enhancing the scalp immune barrier and the vitality of melanocytes, and its preparation method and application, belonging to the technical fields of microbial fermentation and skin immunity. The preparation method of the plant fermentation composition comprises the following steps: using the water extracts of Polygonum multiflorum Thunb., Asparagus cochinchinensis (Lour.) Merr. and black tea as substrates, and fermenting with Wickerhamomyces anomalus and Saccharomyces cerevisiae; the mass ratio of Wickerhamomyces anomalus to Saccharomyces cerevisiae is 3-10:1; the weight ratio of Polygonum multiflorum Thunb., Asparagus cochinchinensis (Lour.) Merr. and black tea is 10-30:1-10:1. The present invention utilizes microbial fermentation technology to provide a cosmetic raw material that promotes melanogenesis by enhancing the innate immune response of melanocytes, and solves the technical problem of black hair turning white. Applying the cosmetics of the present invention can improve the health of hair and scalp, and has a good effect of blackening hair, effectively preventing hair from turning grayish-white.

The present application relates to the field of food technology, and specifically discloses a composition for reducing gastroesophageal reflux in individuals, a preparation method and application thereof. The composition for reducing gastroesophageal reflux in individuals comprises beta-defensin 1, lysozyme and lactoperoxidase. The present application constructs a multi-target synergistic intervention mechanism combining physical protection, secretion regulation and tissue repair through the compounding of four core components, which can comprehensively block the occurrence and development of gastroesophageal reflux. Lactadherin constructs a physical barrier to reduce the risk of reflux dynamics from the source; MLCT precisely regulates gastric acid secretion to reduce chemical stimulation; HMO complex IgG strengthens the mucosal immune barrier and promotes damage repair. The above composition is applied to products for reducing gastroesophageal reflux in individuals, and multi-target synergistic effect is achieved, realizing a full-chain closed-loop intervention from prevention, relief to repair, and significantly improving the clinical improvement effect.

The invention belongs to the technical field of foods, and particularly relates to a high-biological-activity muscle-building formula food for middle-aged and elderly people and a preparation method thereof. According to the high-biological-activity muscle-building formula food for the middle-aged and elderly people, the prepared mulberry leaf extract is added, the raw material raw milk is subjected to multi-stage membrane filtration to enrich beta-lactoglobulin, other auxiliary materials are adopted, muscle attenuation can be delayed, blood sugar can be remarkably reduced, sufficient nutrition support can be provided for the middle-aged and elderly people, and the high-biological-activity muscle-building formula food is suitable for the middle-aged and elderly people. The content of beta-lactoglobulin in the formula is not less than 3%, so that muscle health can be effectively improved, the immune barrier is improved, and functional decline is delayed.

The invention relates to a moisturizing and wrinkle fading composition and application thereof. The moisturizing and wrinkle fading composition is prepared from the following components: beta-glucan, dipalmitoyl hydroxyproline, butyrospermum parkii fruit fat unsaponifiable matter, sodium hyaluronate and soluble collagen. The beta-glucan, the dipalmitoyl hydroxyproline, the butyrospermum parkii fruit fat unsaponifiable matter, the sodium hyaluronate and the soluble collagen are creatively combined, and the beta-glucan can activate the skin immune barrier and create a stable environment for repairing; sodium hyaluronate and butyrospermum parkii butter unsaponifiable substances can maintain skin moisture and reduce dry wrinkles; the dipalmitoyl hydroxyproline can stimulate collagen regeneration, and the soluble collagen provides a repairing raw material at the same time, so that the effects of long-acting moisturizing and deep wrinkle fading are achieved. The five functional components cooperate with one another and have a synergistic effect, the moisturizing and wrinkle fading effects are jointly achieved, and the problems that lips are dry and wrinkles are likely to be generated can be better solved.

The present application belongs to the technical field of drug delivery, and relates to a sequential multi-stage targeted delivery system of a probiotic and prebiotic combination for repairing intestinal barriers. The delivery system is a microcapsule MP wrapped with NPs; the NPs are solid lipid nanoparticles formed by wrapping a probiotic with hyaluronic acid coupled small molecule acids as a film material; and the shell material of the microcapsule MP is a prebiotic. By wrapping the probiotic with hyaluronic acid coupled small molecule acids as a film material to form NPs, and then wrapping the NPs with a prebiotic wall material to form a microcapsule, the sequential multi-stage targeting and zonal release of different drugs can be achieved; the repair of the barrier will not have a reverse effect; the intestinal epithelial cell proliferation is promoted to repair the mechanical barrier; the intestinal flora abundance is improved to repair the microbial barrier; the mucus secretion is promoted to repair the chemical barrier; and the immune homeostasis is regulated to repair the immune barrier.

The invention provides a natural plant feed additive for improving immunity and promoting growth as well as a preparation method and application thereof, and relates to the field of feed additives, the natural plant feed additive is prepared from fourteen plant raw materials including gynostemma pentaphyllum, scandent hop, gentiana macrophylla, glossy privet fruit, flatstem milkvetch seed, sargentgloryvine stem, sea-buckthorn, astragalus membranaceus, radish seed, Chinese violet, humifuse euphorbia herb, ampelopsis japonica, paulownia flower and platycodon grandiflorum. The additive has multi-dimensional biological activity of promoting growth, enhancing immunity, resisting oxidation and protecting organs, can improve the growth performance of livestock and poultry by improving the average daily gain and reducing the feed-gain ratio to optimize the feed conversion rate, and can construct a humoral immune barrier by reducing the secretion of proinflammatory factors and increasing the level of anti-inflammatory factors to improve the immunity of livestock and poultry. According to the present invention, with the application of the composition, the protein and lipid metabolism disorder can be improved, the activity of ALT, AST and ALP can be reduced so as to protect the liver and the kidney and maintain the steady state of the body, the overall health level of livestock and poultry can be improved without the dependence on chemical agents, and the reliable technical support can be provided for promoting the development of the green, safe and efficient livestock and poultry breeding mode.

The invention discloses a modular combined vaccine soluble microneedle patch based on a mechanical interlocking tenon-and-mortise structure as well as a preparation method and application of the modular combined vaccine soluble microneedle patch. According to the combined and spliced micro-needle vaccine patch designed by the invention, synchronous delivery of various vaccines is realized by virtue of a micro-needle technology through fragmented loading and mechanical interlocking'mortise and tenon 'structural design, mutual interference among different vaccines is reduced at the same time, efficient protection can be provided for groups needing multi-vaccine protection by virtue of one-time inoculation, the number of inoculants is reduced, and the immune procedure is simplified; vaccine hesitation is reduced, and inoculation rate is increased; meanwhile, the biosoluble material of the microneedle structure can quickly release antigens in the skin to stimulate immune cells to aggregate so as to enhance the immune effect; the micron-sized needle array (lt; 500 m) acts on the superficial layer of the skin, is painless and can be designed into a self-adhesive patch, so that the inoculation compliance is improved; single-dose inoculation can also reduce medical cost, is suitable for resource-deficient regions, improves accessibility and burdenability, and accelerates establishment of a population immune barrier.

The invention belongs to the technical field of biological medicine, and particularly relates to a preparation method of a probiotic hydrogel material and application of the probiotic hydrogel material in treatment of acute radioactive intestinal injury. In order to solve the clinical key problem of radioactive intestinal injury, a novel biological material TG-CCS-coated DY802 of thiolated synchronously modified chitosan hydrogel TG-CCS loaded with'localized high-response 'lactobacillus salivarius DY802 is constructed. The synthesis process of the material is synergistic and time-saving, has excellent biological safety and tumor inhibition experiment performance, and can comprehensively repair four barriers of the intestinal tract: ROS is removed, and tight junction protein is protected to repair the physical barrier; forming a hydrogel protective layer to reinforce the chemical barrier; the flora diversity is improved, and the prebiotic effect is exerted to repair the biological barrier; the sphingomyelin metabolism and ceramide abundance are regulated and controlled, the abnormal infiltration of NK cells is inhibited, and the immune barrier is remodeled. Therefore, the probiotic hydrogel material disclosed by the invention is clear in treatment mechanism and remarkable in clinical transformation potential and application value.

The present invention relates to a chimeric antigen receptor containing an antigen-binding site that specifically binds to HLA-G, an immune barrier, an immune cell into which the receptor has been introduced, and uses thereof. The immune cell can efficiently remove cancer cells expressing the immune barrier, and is therefore expected to activate endogenous immune cells that have been suppressed by the immune barrier, and therefore can be usefully used as an immunotherapy method for various cancer diseases associated with HLA-G.

The invention discloses a multi-target anticancer composition based on space-time controlled release and synergistic effect and a preparation method thereof, the composition is composed of three oral granules with synergistic functions: immune basic granules used for establishing an immune barrier; the targeted controlled-release particles are used for targeted release of the CD47 inhibitor and the flora prodrug in the intestinal tract; the space-time programming particle is used for carrying out in-vivo epigenetic editing under light control. Through dosage form design and a multi-level controlled release technology, time sequence and space precise treatment under oral administration is achieved, the medicine has the advantages of being high in synergy, high in targeting performance, good in safety and good in patient compliance, and a brand new solution is provided for radical treatment of cancers.

The present invention relates to a fusion protein, nucleic acid molecule, biological material, application and product co-expressing porcine EGF, Ghrelin and IGF-1, belonging to the field of biotechnology, including porcine EGF, Ghrelin and IGF-1. The fusion protein is as follows: (a1) consisting of the amino acid sequence shown in SEQ ID NO:1; (a2) obtained by connecting a tag to the N-terminus and / or C-terminus of the amino acid sequence; (a3) having an identity of 80-99% or more with (a1) or (a2); (a4) substituting, deleting and / or adding one or several amino acid residues to (a1) or (a2). The present invention constructs a fusion gene of porcine EGF, Ghrelin and IGF-1, integrates it into an expression system to obtain a fusion protein, and explores the immunobiomodulatory effect of the fusion protein in vitro, the anti-infection activity in mice and the immunomodulation in pigs and other biological effects; it is found that the fusion protein can significantly enhance the immune barrier function of the animal digestive tract mucosa, improve its systemic immune level and antibacterial infection ability, and has good biological effects in promoting animal growth and development.

The invention relates to the technical field of biological medicines, in particular to nicotinamide-ceramide compounds and application thereof in skin care products. The nicotinamide-ceramide compound disclosed by the invention has better effects in the aspects of resisting aging, and / or moisturizing, and / or enhancing skin immune barrier, and / or relieving skin, and / or resisting allergy, and / or whitening, and / or inhibiting bacteria, and can be used for preparing cosmetics, health care products or medicines with corresponding effects or indications.

The invention provides a 10-hydroxydecanoic acid derivative as well as a preparation method, cosmetics and application thereof, and relates to the technical field of biological medicines. The structure of the 10-hydroxydecanoic acid derivative is shown as a formula I, R is selected from substituted or unsubstituted C10-C18 alkyl, and a substituent group is selected from at least one of hydroxyl and unsaturated bonds. The invention provides a ceramide compound with a new structure, which can resist allergy, relieve itching, resist inflammation and improve skin immune barrier, and has a remarkable effect in hair care.

The invention belongs to the technical field of cosmetics, and particularly relates to a repairing composition containing camellia nitidissima extract microcapsules and application of the repairing composition. The repairing composition containing the camellia nitidissima extract microcapsules comprises the camellia nitidissima extract microcapsules, hydrolyzed royal jelly protein, chamomile extract, European foxtail millet leaf extract and magnolia sieboldii extract which have a synergistic effect, and the aim of barrier repairing can be achieved from multiple aspects of a physical barrier, an immune barrier, a microbial barrier and the like. The stability, the safety and the comprehensive effect of the composition are improved. According to the camellia nitidissima extract microcapsule disclosed by the invention, the camellia nitidissima extract is wrapped by using the inulin as a wall material, so that the thermal stability can be enhanced, water absorption and moisture retention of skin can be effectively promoted, the problems of skin dryness, tightness and the like can be relieved, harmful bacteria can be inhibited, and the growth of beneficial bacteria can be promoted. The composition provided by the invention also has the advantages of being simple and convenient in preparation method, suitable for industrial production and the like, can be applied to preparation of repair cosmetics, and has better market application value.

The invention belongs to the field of cosmetics, and particularly relates to a synergistic repair composition for sensitive skin multi-barrier damage and application of the synergistic repair composition. The invention relates to a synergistic repair composition aiming at damaged multiple barriers of sensitive skin. The synergistic repair composition comprises the following components in parts by weight: 10-25 parts of a physical barrier functional component, 3-10 parts of an immune barrier functional component, 3-10 parts of a microbial barrier functional component and 56-84 parts of a solvent. According to the technology, starting from a skin barrier damage mechanism, through screening and scientific matching of the functional components, the synergistic interaction effect of repairing the damaged skin barrier is generated among the components while the self effects of the functional components are fully exerted.

The invention discloses a composite peptide premix feed capable of improving immunity of livestock and poultry aquatic products and a preparation method thereof, and relates to the technical field of feeds. In order to reduce the use amount of antibiotics in the livestock breeding process, a composite peptide component is added into the premix feed, and the use amount of the antibiotics is reduced by adding bovine lactoferrin peptide in the premix feed. Macrophages and natural killer cells are activated, immune globulin secretion is promoted, and the mucosal immune barrier function is enhanced, so that the autoimmunity of livestock is improved, and the disease resistance of the livestock is improved; and in order to avoid the defect that the feed is easy to mildew to generate mildew toxins, mildew-proof modified attapulgite clay is also prepared and added, so that the mildew speed after the premixed feed and the feed are mixed and watered is effectively slowed down, and the diarrhea rate of livestock is reduced.

The application discloses a Bifidobacterium longum subsp. longum strain for regulating intestinal homeostasis and relieving intractable constipation and an application thereof, and belongs to the field of microorganisms. The strain CCFM1319 provided by the application can shorten the time of the first black stool of intractable constipation mice, significantly enhance the colon peristalsis ability of the intractable constipation mice, increase the expression level of the characteristic marker of enteric glial cells in the colon tissue, repair the intestinal nerve damage caused by intractable constipation, reduce the expression level of inflammatory factors in the colon tissue of the intractable constipation mice, relieve the inflammation of the intestinal immune barrier, reduce the pathological damage of the colon tissue, increase the expression amount of the tight junction protein in the colon tissue, repair the damage of the intestinal mechanical barrier, avoid excessive water absorption, be beneficial to the excretion of feces, regulate the intestinal chemical barrier, improve the intestinal microecological imbalance, make the intestinal flora structure tend to be healthy, and repair the damage of the intestinal biological barrier, so as to play a role in comprehensively relieving intractable constipation.

The invention provides a sensitive skin barrier repairing composition as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. Comprising the following components in percentage by weight: 1.5-5% of a polysaccharide component, 0.0001-0.01% of a peptide component, 0.1-1% of a carotenoid component, 3-10% of an unsaturated fatty acid component, 1-3% of an algae extract component and the balance of a matrix component, totaling 100%. The sensitive skin barrier repairing composition prepared by the invention systematically repairs a physical barrier, a chemical barrier, an immune barrier and a micro-ecological barrier of skin by simultaneously acting on a plurality of biological targets related to sensitive skin, so that uncomfortable symptoms such as skin redness, pruritus, scorching hot and the like are efficiently relieved.