190 results about "Melanocyte" patented technology

The invention provides application of UBR5 in preparation of drugs for treating pigmented skin diseases, and further the pigmented skin diseases comprise various types of pigmented nevus (mainly congenital pigmented nevus and megalous with the risk of malignant transformation). Various pigmented spots (mainly chloasma, freckles, Taitian nevus, post-inflammation hyperpigmentation and the like) and hypopigmentation / depigmentation spots (mainly leucoderma, post-inflammation hypopigmentation and the like). Experimental results show that UBR5 is highly expressed in disease tissues such as pigmented nevus. The UBR5 expression level and the melanogenesis ability are in significant positive correlation, targeted knockout / knockdown of UBR5 in melanocytes can significantly inhibit the expression level of melanogenesis related genes such as MITF, TRP1, TRP2, TYR and other proteins, the melanin content of melanocytes and tyrosinase activity, vice versa, and the melanogenesis ability of melanocytes after overexpression of UBR5 is significantly increased.

Provided are a whitening and freckle-removing composition, a gel, a preparation method therefor, and a use thereof, relating to the technical field of skin-care products. The whitening and freckle-removing composition comprises tranexamic acid, nicotinamide, 4-butylresorcinol, licorice extract, 3-o-ethyl ascorbic acid, and glutathione. For the whitening composition, by inhibiting the activity of tyrosinase, blocking the transfer of melanin from melanocytes to keratinocytes, and inhibiting the formation and accumulation of melanin by means of multiple mechanisms including oxidation resistance, and by means of the synergistic action of all components, cosmetic benefits of whitening and freckle-removing are achieved. The whitening and freckle-removing gel has a simple preparation method, has a mild and non-sensitizing formula, is safe and non-irritating, and is suitable for large-scale production and application.

The invention provides a whitening composition, a whitening product, a preparation method and application. The invention provides application of the milk exosome and nicotinamide in improving the autophagy ability of tranexamic acid activated melanocytes. The whitening composition provided by the invention is prepared from the following components in parts by weight: 1 to 50 parts of milk exosome, 5 to 40 parts of tranexamic acid and 0.5 to 30 parts of nicotinamide. The provided whitening composition is an active composition capable of activating the autophagy function of melanocytes of a human body, melanin synthesis in the melanocytes can be reduced by inducing generation of autophagy-related protein and activating an autophagy system, dullness of the skin is improved, the skin color is brightened through an internal channel, and the product is free of irritation and high in safety.

The invention provides a tyrosinase inhibitor compound as shown in a formula (I), a composition as well as a preparation method and application of the tyrosinase inhibitor compound. The compound not only has excellent tyrosinase inhibitory activity, but also has good physicochemical properties, can meet the use requirements of people at an extremely low concentration, and has no toxicity to melanocytes or skin.

The present disclosure relates to novel peptide antagonists that inhibit binding of melanocyte stimulating hormone to the melanocortin 1 receptor. The peptide antagonists of the disclosure are useful in cosmetic compositions that prevent or reduce the appearance of skin discoloration caused by pigmentation. The disclosure further relates to cosmetic compositions comprising a peptide antagonist of the disclosure, and methods for their use for preventing or reducing the appearance of skin discoloration in a subject in need thereof.

The present disclosure relates to novel peptide antagonists that inhibit binding of melanocyte stimulating hormone to the melanocortin 1 receptor. The peptide antagonists of the disclosure are useful in cosmetic compositions that prevent or reduce the appearance of skin discoloration caused by pigmentation. The disclosure further relates to cosmetic compositions comprising a peptide antagonist of the disclosure, and methods for their use for preventing or reducing the appearance of skin discoloration in a subject in need thereof.

The application provides a tyrosinase-based transdermal delivery system for treating vitiligo, which is a core-shell structure, the core is tyrosinase, and the shell is a polymer shell layer. The application coats a polymer protective layer on the surface of the tyrosinase through in-situ polymerization reaction on the double bond of the tyrosinase, can effectively protect the activity of the tyrosinase and promote the transdermal delivery of the tyrosinase, and can control the targeted delivery of the tyrosinase nanogel to melanocytes in the deep part of the skin and hair follicle by adjusting the polymerization conditions of the polymer shell, so as to promote the in-situ synthesis of melanin. The transdermal delivery system of the application can also load active small molecules with anti-inflammatory function in the center of the tyrosinase, and the small molecules are released after being targeted and delivered to the inflammation area of vitiligo along with the tyrosinase nanogel, so as to relieve inflammation and protect melanocytes from damage.

The invention discloses preparation and application of an umbilical cord extract capable of blacking hair by regulating melanocytes of hair follicles to generate melanin particles. The preparation method of the umbilical cord extract comprises the following steps: washing an animal umbilical cord, cutting the animal umbilical cord into pieces, digesting the animal umbilical cord with pancreatin, adding a serum-free mesenchymal stem cell culture medium, blowing and beating to disperse tissues, filtering, performing low-energy red light irradiation on the filtered tissue fluid, incubating for 6-12 hours, taking out the tissue fluid, performing freeze thawing treatment, performing ultrasonic treatment, filtering, diluting the filtrate with sterile water, and performing freeze drying to obtain the umbilical cord extract. The umbilical cord extract is obtained. The umbilical cord extract disclosed by the invention has a relatively good regulating effect on melanocyte activity and melanin generation capability, can increase the content of melanin particles in hair, and can be used as a cosmetic, a raw material or a medicine with a hair-blacking effect.

The invention discloses preparation and application of a whitening and repairing composition containing temperature-sensitive oxidized resveratrol hydrogel, and relates to the technical field of cosmetics. The composition comprises the following components in parts by weight: 1-5 parts of temperature-sensitive oxidized resveratrol hydrogel, 4-6 parts of a peucedanum praeruptorum dunn extract, 1-3 parts of a willowleaf swallowwort rhizome extract and 0.1-0.5 part of a tephrosia rubescens seed extract. The combination of the peucedanum praeruptorum dunn extract, the cynanchum wilfordii extract, the tephros glauca seed extract and the oxyresveratrol finds that the composition has a synergistic interaction effect, and the composition formed by the components through specific compatibility has the effects of whitening, repairing and improving skin elasticity. The oxidized resveratrol is wrapped by the temperature-sensitive hydrogel, so that the oxidized resveratrol is kept in a gel state at an epidermal cuticle layer, and after the oxidized resveratrol permeates into a skin basal layer, the oxidized resveratrol can be released at the skin basal layer, acts on melanocytes more effectively, inhibits tyrosinase and further affects generation of melanin.

The invention provides an application of alpha-melanocyte stimulant in treating asthma by inhibiting type II innate lymphocytes. An IL-7R-STAT pathway in activated lung ILC2 is inhibited through an alpha-MSH-MC5R axis, it is found that the neuroendocrine hormone alpha-MSH can target ILC2 to limit lung inflammation, and screening of new drugs for treating lung inflammation is facilitated.

A composition capable of preventing or improving skin aging by controlling a senescence biomarker of melanocytes is disclosed. The composition includes a thymol ester-based compound as an effective ingredient, whereby when applied to a subject in which skin cells are flattened or enlarged, or β-galactosidase of melanocytes is activated, it can exhibit the effect of preventing or improving skin aging.

The invention relates to a composition with whitening and skin tendering effects, essence lotion and a preparation method and application of the essence lotion, in the composition with the whitening and skin tendering effects, the weight ratio of achillea millefolium extract to hydroxyethyl piperazine ethanesulfonic acid to ormosia henryi bark extract is (0.1-1): (0.5-8): (0.05-0.5). The essence lotion is prepared from the following components in percentage by weight: 0.75 to 6 percent of composition with skin whitening and tendering effects, 3 to 8 percent of emollient, 0.5 to 2 percent of emulsifier, 0.01 to 1 percent of filler, 0.5 to 2 percent of thickener, 0.8 to 1.5 percent of preservative, 0.02 to 0.2 percent of daily essence, 0.1 to 10 percent of skin conditioner, 0.1 to 10 percent of humectant and the balance of deionized water. The main components have a synergistic effect, can effectively regulate and control the information network effect of endothelin and reduce the differentiation, proliferation and the like of melanocytes in the first step of melanin generation by inhibiting the activity of tyrosinase, can promote cell autophagy and effectively metabolize melanin and lipofuscin generated in the skin, and achieves the effects of whitening, whitening, whitening and the like. The skin tendering effect is realized.

The invention discloses a plant fermentation composition for enhancing scalp immune barrier and melanocyte activity and a preparation method and application of the plant fermentation composition, and belongs to the technical field of microbial fermentation and skin immunization. Performing fermentation by utilizing Wickerhamomyces anomalus and saccharomyces cerevisiae; the mass ratio of the Wickerhamomyces anomalus to the saccharomyces cerevisiae is (3-10): 1; the weight ratio of the radix polygoni multiflori to the radix asparagi to the black tea is (10-30): (1-10): 1. The invention provides a cosmetic raw material for promoting melanogenesis by enhancing innate immune response of melanocytes by utilizing a microbial fermentation technology, and solves the technical problem that black hair becomes white. The cosmetic provided by the invention can improve the health of hair and scalp, has a good hair blackening effect, and effectively prevents the hair from becoming grey and white.

The invention provides a medicinal and edible composition for treating vitiligo as well as a preparation and application thereof, and the medicinal and edible composition comprises the following components in parts by weight: 1-8 parts of peach kernel, 1-8 parts of galangal, 1-8 parts of angelica dahurica and 1-8 parts of black sesame. According to the invention, the peach kernel, the galangal, the radix angelicae and the black sesame which are homologous in medicine and food are combined, so that the effect of treating leucoderma is exerted. Animal experiments show that the effective rate of the peach kernel, galangal, angelica dahurica and black sesame medicine composition for treating vitiligo can reach 70%, the content of tyrosinase in serum of vitiligo model guinea pigs can be increased, synthesis of melanin is accelerated, meanwhile, the content of alpha-melanin cell stimulant in serum of the vitiligo model guinea pigs can be increased, and the curative effect is good. The generation of skin melanin is promoted, and melanin cells are protected.

The invention discloses a melanocyte dissolution and separation device and a use method. The melanocyte dissolution and separation device comprises a case and a lifting assembly, a central shaft is rotationally connected in the case, a placement groove is formed in the upper end of the central shaft, supporting seats which are uniformly distributed are arranged at the upper end of the outer arc surface of the central shaft, rotating shafts are rotationally connected to the ends, away from the central shaft, of the multiple supporting seats, test tube seats are arranged outside the multiple rotating shafts, and adjustable tube seat assemblies are movably clamped to the bottoms of the test tube seats; according to the melanocyte dissolution and separation device and the use method, the test tube seat is provided with the adjustable base, so that test tubes with different lengths can be conveniently placed, the test tubes can be automatically clamped after being inserted, the angle of the test tube group is adjustable, the use flexibility is good, and the test tube group is convenient to use and high in practicability. Supernate in a plurality of test tubes can be discharged synchronously, and the use convenience is good.

A smartphone-based hemoglobin (Hgb) assessment application quantitatively analyzes pallor in patient-sourced photos using image analysis algorithms to enable a noninvasive, accurate quantitative smartphone app for detecting anemia. A user takes a photo of his / her fingernail beds using the app and receives an accurate displayed Hgb level. Since fingernails do not contain melanocytes, the primary source of color of these anatomical features is blood Hgb. At the same time, quality control software minimizes the impact of common fingernail irregularities (e.g. leukonychia and camera flash reflection) on Hgb level measurement. Metadata recorded upon capturing the image is leveraged for determining a users' Hgb level thereby eliminating the need for external equipment. A personalized calibration of image data with measured Hgb levels improves the accuracy of the application.

The invention discloses a method for regulating the body color of ornamental fish, and belongs to the technical field of aquaculture. According to the method disclosed by the invention, the aquarium fishes in the fry stage and the juvenile stage are soaked with thyroid hormone, so that the excessive thyroid hormone can obviously reduce the number of haematochrome cells and the size of pigment particles of the aquarium fishes in different stages, and reduce the content of carotenoid; when the synthesis of thyroid hormone is inhibited, gene expression of melanocytes and related synthetic pathways can be promoted, and meanwhile, the carotenoid level is increased. The inhibition effect of thyroid hormone on scatophagus argus haematochrome cell development and carotenoid accumulation is determined for the first time, the knowledge blank in the field is filled, and important guidance information is provided for body color regulation and genetic improvement of aquarium fish and other aquatic animals in the future.

The invention relates to the technical field of hair treatment medicines, and particularly discloses an anti-alopecia hair-blacking liquid for nourishing white hair and blacking hair and a preparation method of the anti-alopecia hair-blacking liquid. The hair loss preventing and blackening liquid for white hair and black hair comprises the following raw materials in parts by mass: 5-10 parts of kaempferol, 1-5 parts of aromatic folic acid, 1-2 parts of lecithin, 1-2 parts of a surfactant, 40-60 parts of vegetable oil, 1-3 parts of blue copper peptide, 1-2 parts of dopamine hydrochloride, 0.01-0.1 part of 1-ethyl-(3-dimethylaminopropyl) carbodiimide and 1-2 parts of a traditional Chinese medicine extract. Hydrophilic and lipophilic active components are loaded at the same time, repair and development of melanocytes are effectively promoted, connection of hair papilla and blood vessels can be enhanced, the hair growing effect is remarkable, irritation is small, hair follicles can be deeply nourished, dependence cannot be generated, and the composition is suitable for large-scale application and popularization.

The present invention relates to compounds, compositions and methods for modulating skin pigmentation and treating or preventing UV-induced skin injury, erythema, skin aging, sunburn and hyperpigmentation in a subject. The compounds, compositions, and methods of the invention generally relate to malassezia derived compounds, including malassezia and indirubin, and / or chemical analogs thereof. Other applications of the compounds and compositions disclosed herein include, but are not limited to, ameliorating hyperpigmentation caused by hyperpigmentation conditions, inducing apoptosis of melanocytes, inducing apoptosis of melanocytes, and inducing apoptosis of melanocytes. And modulate aromatic hydrocarbon receptor (AhR) activity, melanogenesis, melanin production, melanosome biosynthesis, melanosome transfer, melanocyte activity, and melanin concentration.

The peptides of the present invention exhibit skin whitening activity by inhibiting melanin synthesis in melanocytes and inhibiting melanin body migration. Therefore, the peptide of the present invention can be used as an active ingredient in a medicament for treating or preventing hyperpigmentation diseases caused by excessive deposition of melanin bodies, or as an active ingredient in a cosmetic for skin whitening.

The invention discloses a preparation method of drug-loaded membrane protein bionic vesicles based on a microfluidic technology and application of the drug-loaded membrane protein bionic vesicles to targeted melanocyte delivery drugs, and belongs to the technical field of pharmaceutical preparations. The method is easy and convenient to operate, assembly of the phospholipid bilayer and insertion of the membrane protein are accurately controlled through the laminar focusing technology, and the stable and efficient drug-loaded membrane protein bionic vesicle is formed. The drug-loaded membrane protein bionic vesicles can effectively target melanocytes, promote the uptake of the vesicles, significantly improve the accumulation of drugs in the melanocytes, and enhance the treatment effect on melanocyte related diseases (such as hyperpigmentation, melanoma, vitiligo and the like). Meanwhile, the drug-loaded bionic vesicles constructed by the invention have relatively low keratinocyte uptake rate, drug uptake of non-targeted tissues is effectively reduced, and side effects of drugs are reduced, so that the drug-loaded membrane protein bionic vesicle system constructed by the invention has great prospects in the aspect of treating melanocyte related diseases.

The invention relates to a method for rapidly preparing vernonia anthelmintica dealicin from vernonia anthelmintica dealicin, which comprises the following steps: carrying out ultrasonic extraction by using ethanol, enriching by using macroporous resin to obtain a vernonia anthelmintica dealicin extract, and carrying out extraction and pilot plant test preparative liquid chromatography separation to obtain a high-purity vernonia anthelmintica dealicin compound. The results of subsequent researches show that the vernonia anthelmintica dealicin can significantly inhibit the secretion of the chemotactic factor, and the inhibition rate of 1mol / L of vernonia anthelmintica dealicin on the release level of the chemotactic factor CXCL10 reaches 75.83%. The vernonia anthelmintica macrobrachin reduces the recruitment of self-reactive T cells to the skin by blocking a CXCL10-mediated inflammation signal channel, so that melanocytes are protected, and pigment regeneration is promoted. Therefore, the vernonia anthelmintica macrobrachin inhibits the secretion of the chemotactic factor CXCL10 in a targeted manner, can be used as a potential effective monomeric compound in the development of vitiligo medicines for research, and is expected to become a new strategy for treating vitiligo.

This invention discloses the use of ONO-2952 or a pharmaceutically acceptable salt thereof in the preparation of drugs / cosmetics for treating and / or preventing hyperpigmentation disorders, belonging to the field of pharmaceutical technology. ONO-2952 of this invention inhibits melanin synthesis induced by endogenous factors α-MSH, ACTH, and UVB radiation, thereby inhibiting melanin synthesis by suppressing the expression of key melanin synthesis proteins MITF and Tyrosinase in melanocytes. Therefore, ONO-2952 or a pharmaceutically acceptable salt thereof can be used in the preparation of drugs / cosmetics for treating and / or preventing hyperpigmentation disorders.

The present invention relates to a composition comprising plant extracts. The composition according to the present invention has a skin whitening effect by reducing the total amount of melanin and tyrosinase activity in melanocytes of the skin, promotes skin regeneration and increases skin elasticity or reduces skin wrinkles by promoting collagen synthesis and inhibiting collagenase activity in fibroblasts of the skin, has an anti-inflammatory effect or a skin soothing effect by inhibiting NO generation, increases the amount of moisture in the skin and has a moisturizing effect by promoting generation of hyaluronic acid in fibroblasts, and has an antioxidant effect by scavenging free radicals. In addition, since the composition of the present invention exhibits a broad antibacterial effect against various bacteria, the composition may be used as a cosmetic composition, a pharmaceutical composition, a skin external preparation, or a food composition.

The present disclosure discloses an engineered liposome with cell membrane proteins to reduce melanosome transport and a preparation method thereof, and belongs to the technical field of cosmetics and biomedicine. The present disclosure provides the engineered liposome with cell membrane proteins to reduce melanosome transport and the preparation method thereof, which is easy to operate, requires no large-scale equipment, has few additives, and a preparation process is simple and environmentally friendly. The biomimetic liposome can significantly inhibit melanin transport. The fluorescence intensity of melanosomes in keratinocytes is found to decrease by 3.5-fold in a co-culture test of melanocytes and the keratinocytes, indicating that this biomimetic liposome is very effective in inhibiting accumulation of melanin in skin keratinocytes. These findings provide an effective strategy for reducing melanosome transfer to treat hyperpigmentation, while also introducing an alternate approach for regulating cellular communication of extracellular vesicles and organelles.

The invention discloses a whitening composition as well as a preparation method and application thereof. The whitening composition is prepared from the following components: a tea leaf extract, a lavender flower extract and water, wherein the tea leaf extract, the lavender flower extract and the water are added into the whitening composition. The whitening composition can interact and synergistically improve the autophagy ability of cells, synergistically improve the phagocytosis of melanin in keratinocytes and the removal ability of skin metabolic wastes, can synergistically inhibit the generation of melanin and synergistically improve the expression of miR-137 genes in melanocytes, has a relatively high development prospect of cosmetics with whitening effects, and has a wide application prospect. Moreover, the composition is mild in property, easy to absorb and non-irritant, and can be widely applied to skin care cosmetics.

The invention discloses application of an ATP (adenosine triphosphate) citrate lyase (ACLY) inhibitor in preparation of a medicine for treating leucoderma, and belongs to the technical field of preparation of medicines for treating leucoderma. Through targeted inhibition of ACLY activity or specific knockout of ACLY in CD8 + T cells, abnormal activation of ACLY and secretion of killing effector molecules (Granzyme B, IFN-gamma, Perforin and the like) are inhibited, and infiltration of the CD8 + T cells to skin lesion parts is reduced, so that melanocytes are protected from immune injury, and the progress of vitiligo is blocked. The application is based on the abnormally high expression of ACLY in CD8 + T cells of vitiligo patients and the close association with disease activity, and a brand new targeted treatment thought and a medicine research and development direction are provided for vitiligo.

The present application relates to azapeptide compounds of formula (I) derived from melanocyte-stimulating hormone release-inhibiting factor-1. A method to obtain compounds of formula (I) is also described herein. The compounds of formula (I) have been shown to be suitable for the treatment of dopamine-related disorders of the central nervous system. These compounds showed enhanced potency as positive allosteric modulators of the dopamine D2 receptors in comparison with the melanocyte-stimulating hormone release- inhibiting factor-1 and, in general, do not exhibit meaningful cytotoxicity.

The invention relates to hair-blacking essence and a preparation method thereof. Screening a symbiotic bacterium metabolism extract with small molecule peptide metabolism regulation capability; by combining low-concentration azelaic acid and zinc lactate components, scalp grease oxidation and abnormal proliferation of propionibacterium are inhibited; the expression of IL-10 anti-inflammatory factors is induced, the expression of TNF-alpha and IL-6 is reduced, and the hair follicle microinflammation state is reduced; a Wnt / beta-catenin pathway activator is used for inducing the melanin stem cells to be in a static state; the FGF-2 and the EGF are combined in a micro-dose manner to promote the amplification into precursor cells; mITF regulation peptides and a small amount of trace copper ions are supplemented to induce directional differentiation into mature melanocytes; a microcapsule carrier system with a three-layer structure; the penetration efficiency is improved; antioxidative peptide rich in reduced glutathione, NAD and a promoting factor are used, so that melanocytes are protected from oxidative damage; a coenzyme Q10 type mitochondrial homeostasis regulator is introduced, so that the energy metabolism is improved, the cell aging is delayed, the targeting property is strong, and the effect is accurate.

The invention provides siRNA lipid nanoparticles for targeted therapy of chloasma as well as a preparation method and application of the siRNA lipid nanoparticles, and belongs to the technical field of biological medicines. The invention provides LNP-siRNAs for treating chloasma. The LNP-siRNAs for treating chloasma comprise siRNAs of key molecules for targeted inhibition of melanin formation. According to the invention, an effective siRNA sequence is screened out and verified in cells, then siRNAs with an obvious inhibition effect are packaged into LNP-siRNAs, the packaging quality of the LNP-siRNAs is evaluated, and the LNP-siRNAs are applied to cells and animals to evaluate the inhibition effect of the LNP-siRNAs on melanin. The packaged LNP-siRNAs are used for melanocytes and animal skin, can effectively inhibit the formation of melanin, and can be used for screening and preparing potential medicines for clinically treating chloasma.