43 results about "Melanocyte" patented technology

The peptides of the present invention exhibit skin whitening activity by inhibiting melanin synthesis in melanocytes and inhibiting melanin body migration. Therefore, the peptide of the present invention can be used as an active ingredient in a medicament for treating or preventing hyperpigmentation diseases caused by excessive deposition of melanin bodies, or as an active ingredient in a cosmetic for skin whitening.

The present invention relates to a composition comprising plant extracts. The composition according to the present invention has a skin whitening effect by reducing the total amount of melanin and tyrosinase activity in melanocytes of the skin, promotes skin regeneration and increases skin elasticity or reduces skin wrinkles by promoting collagen synthesis and inhibiting collagenase activity in fibroblasts of the skin, has an anti-inflammatory effect or a skin soothing effect by inhibiting NO generation, increases the amount of moisture in the skin and has a moisturizing effect by promoting generation of hyaluronic acid in fibroblasts, and has an antioxidant effect by scavenging free radicals. In addition, since the composition of the present invention exhibits a broad antibacterial effect against various bacteria, the composition may be used as a cosmetic composition, a pharmaceutical composition, a skin external preparation, or a food composition.

The invention discloses application of an ATP (adenosine triphosphate) citrate lyase (ACLY) inhibitor in preparation of a medicine for treating leucoderma, and belongs to the technical field of preparation of medicines for treating leucoderma. Through targeted inhibition of ACLY activity or specific knockout of ACLY in CD8 + T cells, abnormal activation of ACLY and secretion of killing effector molecules (Granzyme B, IFN-gamma, Perforin and the like) are inhibited, and infiltration of the CD8 + T cells to skin lesion parts is reduced, so that melanocytes are protected from immune injury, and the progress of vitiligo is blocked. The application is based on the abnormally high expression of ACLY in CD8 + T cells of vitiligo patients and the close association with disease activity, and a brand new targeted treatment thought and a medicine research and development direction are provided for vitiligo.

The present application relates to azapeptide compounds of formula (I) derived from melanocyte-stimulating hormone release-inhibiting factor-1. A method to obtain compounds of formula (I) is also described herein. The compounds of formula (I) have been shown to be suitable for the treatment of dopamine-related disorders of the central nervous system. These compounds showed enhanced potency as positive allosteric modulators of the dopamine D2 receptors in comparison with the melanocyte-stimulating hormone release- inhibiting factor-1 and, in general, do not exhibit meaningful cytotoxicity.

A composition for promoting tyrosinase activity, which includes a peptide consisting of the amino acid sequence of General Formula 1 below: K—Y—R1-R2-R3-R4-R5-R6-R7-R8 (General Formula 1) is disclosed. In General Formula 1, R1 is arginine (R), lysine (K), or glutamine (Q); R2 is arginine (R) or glutamine (Q); R3, R4, and R5 are each arginine (R) or lysine (K); R6 is asparagine (N) or serine (S); and R7 and R8 are each lysine (K) or tyrosine (Y). The composition may promote melanogenesis in melanocytes to prevent, improve or treat hypomelanosis.

This invention discloses a skin-whitening composition that reduces aging melanocytes and its application, relating to the cosmetics field. The invention provides a composition that, by reducing aging melanocytes and decreasing SASP secretion, reduces melanin production and inhibits tyrosinase activity while maintaining healthy melanocytes, thus maintaining skin homeostasis while whitening the skin. This composition can be widely used in various skincare products to effectively achieve long-lasting whitening effects.

The invention discloses a natural spray for promoting blackening of white hair and growth of hair and a preparation method of the natural spray. The spray is prepared by compounding natural plant extracts of polygonum multiflorum, cacumen biotae, black sesame, fructus viticis, angelica sinensis, ginger, ligusticum wallichii, liquorice and the like, and clove or / and cinnamon extract is creatively adopted as a natural preservative flavor enhancer. Through a multi-target synergistic system of nourishing, blood activating and infiltration promoting and activating, anti-inflammation and stability maintaining, hair follicle melanocytes can be nourished from the source, white hair can be naturally turned into black, dormant hair follicles can be effectively activated, and hair growth is promoted. Preferably, ginseng and lucid ganoderma extracts can also be added to enhance the nourishing and stability maintaining effects. The invention aims to overcome the double defects in the prior art, and provides a multifunctional spray which is 100% purely natural and does not contain any chemically synthesized dye, oxidant, hormone, chemical preservative and harmful additive. The invention aims to realize natural blackening of white hair by gradually recovering melanin synthesis of hair follicles through exogenous nutrition supplement, and effectively promote hair growth and prevent hair loss by stimulating scalp microcirculation, resisting inflammation, relieving and directly activating hair papilla cells. The invention finally provides a daily care product which integrates blackening, hair growth and maintenance, is convenient to use (washing-free after spraying) and does not need to be cleaned. All the components are natural, safe and non-irritant, and the shampoo adopts a washing-free spray design, is convenient to use and is suitable for all hair qualities.

The peptides of the invention have a sequence with at least 85% of identity with a sequence SEQ ID NO: 1 or 2. These peptides regulate melanocyte activity in terms of proliferation and production of melanin. Therefore, they are useful in the treatment of melanomas but also of cosmetic cutaneous hyper or hypopigmentation.

The present disclosure relates to biaryl aminoguanidine derivatives of Formula II or a pharmaceutically acceptable salt thereof and processes for their preparation. The present disclosure also relates to the use of such biaryl aminoguanidine derivatives for the treatment of diseases involving the melanocortin receptor or related systems, such as proopiomelanocortin-related diseases.

It has been discovered that differential methylation in DNA can be used to distinguish between malignant and non-malignant skin lesions. Seven genes, 3 that are hypermethylated in melanoma, 1 that is hypermethylated in melanocytes and remains methylated in melanoma, and 3 that are hypermethylated in all tissues are provided as biomarkers. The panel demonstrates a 93% sensitivity, 97% specificity and an AUC of 95% in distinguishing between melanoma and benign lesions. Thus provided herein are melanoma biomarkers and methods of detecting them and using them to diagnose and guide treatment of subjects with the same, and monitor melanoma tumor dynamics during and after treatment.

The invention relates to the technical field of cosmetics, in particular to a mark fading and repairing composition as well as a preparation method and application thereof. The invention relates to a mark fading and repairing composition which comprises the following components in percentage by weight: 0.1%-5% of dragon's blood extract, 0.01%-0.5% of keratinase and 1%-5% of nicotinamide, the preparation method comprises the following steps: preparing the eutectic solvent; performing ultrasonic-assisted extraction; separation and post-treatment; the invention has the following beneficial effects: the resina draconis extract can inhibit continuous pigmentation caused by acne inflammation residue, promote healing of skin minimal wounds and reduce dermis layer injury of acne mark parts; keratinase specifically hydrolyzes stratum corneum aged keratin, cutin metabolism of acne mark parts is accelerated, and accumulation of pigments in stratum corneum is avoided; nicotinamide inhibits melanocytes from transferring melanin to keratinocytes; through the synergistic effect of the three components, a closed-loop printing fading mechanism of anti-inflammatory repair-keratin metabolism-pigment inhibition is formed, the printing fading effect is more comprehensive and more efficient, and skin repair and barrier protection can be both considered.

The invention discloses a composition with a whitening effect. The composition is prepared from the following components in parts by mass: 47 to 51 parts of grape seed oil, 47 to 51 parts of pomegranate seed oil, 0.5 to 0.8 part of 4-butylresorcinol, 0.2 to 0.6 part of VC-IP (Vitamin C-IP) and 0.1 to 10 parts of isorhamnetin-3-O-neohesperidin. The invention also discloses a preparation method of the composition. The invention also discloses an essence oil containing the composition. The invention also discloses a face cream containing the composition. The invention also discloses application of the composition in preparation of cosmetics for skin whitening, spot fading and skin yellowing reduction. The composition provided by the invention establishes a multi-target synergistic pathway: 1, a blackness removal pathway for reducing the activation of oxidative stress such as ultraviolet rays on melanocytes; and 2, a jaundice removal pathway: inhibiting lipid peroxidation and promoting lipofuscin removal.

The invention discloses a preparation method of bionic vesicles simultaneously targeting skin keratinocytes and fibroblasts and anti-aging application of the bionic vesicles, and belongs to the technical field of pharmaceutical preparations and cosmetics. According to the invention, the surface of liposome is modified with melanin cell membrane protein by using a microfluidic technology, and the membrane characteristics of melanin cells are integrated to a vesicle carrier through a biomimetic membrane structure, so that the effect of simultaneously targeting keratinocytes and fibroblasts is realized. The melanin cell membrane protein-derived bionic vesicles prepared by the invention can effectively target skin keratinocytes and fibroblasts, and the specific uptake of the vesicles by the cells is enhanced. After fisetin is loaded, the accumulation of fisetin in skin keratinocytes and fibroblasts can be remarkably improved, so that senescent cells are more effectively removed. Therefore, the bionic vesicles simultaneously targeting the skin keratinocytes and the fibroblasts, which are constructed by the invention, have great application prospects in the field of skin aging treatment.

The invention provides new application of an ATOX1 gene and ATOX1 protein, and belongs to the technical field of treatment of vitiligo diseases. The ATOX1 gene serving as a key gene of copper metabolism is disclosed for the first time, and melanin synthesis is promoted by activating the melanocyte copper metabolism process, so that the effect of resisting leucoderma is achieved. The ATOX1 gene and / or the ATOX1 protein can be used as a key biomarker in the vitiligo morbidity process, and a new target and a new molecular basis are provided for vitiligo research.

The present invention relates to a multimodal non-invasive in vivo system and related method for the characterization of the tissue microenvironment. The system comprises: a multispectral imaging (MSI) camera for superficial spectral screening; a multispectral optoacoustic tomography (MSOT) module for dynamic vascular and perfusion analysis; a Mueller Matrix Polarimetry (MMP) module for extracellular matrix anisotropy assessment; a Raman spectroscopy module for molecular fingerprinting of fibroblast-associated proteins; an optical coherence tomography (OCT) module for morphological and stratigraphic evaluation; an optional Elastic Scattering Spectroscopy (ESS) module for subcellular scattering biomarkers; and a diachronic analysis module for longitudinal monitoring. Data are integrated by an artificial intelligence processing unit to generate quantitative biomarkers of vascularization, extracellular matrix features, immune activity (Langerhans cells) and fibroblast phenotype, distinguishing physiological myofibroblasts from CAF. The invention enables non-nvasive, biopsy-free evaluation of scars, melanocytic lesions, tumor stroma, wound healing and surgical site monitoring.

The application belongs to the technical field of biomedicine, and specifically discloses a gene marker of melanoma and application thereof. The gene marker is a PDP1 gene, and the application proves the correlation between the PDP1 gene and melanoma. The expression amount of the PDP1 gene in melanoma cells is significantly higher than that in normal melanocytes. The PDP1 expression is positively correlated with the pathological stage and the lymph node metastasis condition N value. The PDP1 gene influences the cell proliferation, cell apoptosis, cell migration and cell invasion of melanocytes. Therefore, the PDP1 gene can be used as a potential target for diagnosing and treating melanoma. By detecting the mRNA or protein expression level of the PDP1 gene, melanoma can be detected, and the application has good application value.

The present application relates to the use of a substance that regulates BCL11A gene and / or its encoded product in the preparation of a drug for reversing skin photoaging, wherein the skin photoaging includes skin cell aging caused by sunlight, light, etc., and the skin cells include keratinocytes, dermal fibroblasts, melanocytes. The regulation is to up-regulate the expression of BCL11A gene and / or promote the production of its encoded product. Typical clinical manifestations of the skin photoaging include wrinkles, dry skin, loose skin, dilated capillaries, uneven skin pigmentation, and skin itching. The substance that regulates BCL11A gene and / or its encoded product includes a substance that regulates BCL11A gene DNA molecules and a substance that regulates the expression of BCL11A translation product protein molecules. The reversal of skin photoaging is manifested in reducing the proportion of cells positive for aging-related beta-galactosidase staining in skin cells and reducing the expression of aging-related protein p16 and DNA damage protein gammaH2AX.

The invention discloses a method for analyzing the effect of active pharmaceutical ingredients between T cells and melanocytes in vitiligo. The method comprises the following steps: obtaining transcriptome data; performing differential gene analysis on the transcriptome data to obtain an analysis result; identifying a first gene associated with a disease; determining a second gene by using a machine learning algorithm; drawing a spatial distribution mode of a second gene in the leucoderma skin; carrying out molecular docking on the HSPA1A protein receptor and a small molecule drug ligand, and simulating and predicting a bioactive component for regulating a second gene in the drug; and evaluating the regulation effect of the bioactive components of the drug on the second gene expression pattern, endoplasmic reticulum stress and CD8 + T lymphocyte infiltration by using the animal model, and analyzing the effect of the active components of the drug between T cells and melanocytes. The method provided by the invention can be used for evaluating the effect of the active pharmaceutical ingredient in vitiligo treatment, and particularly determining the effect of the active pharmaceutical ingredient between T cells and melanin cells in vitiligo.

Provided are a tyrosinase inhibitor compound as shown in formula (I) or formula (II), a composition, a preparation method therefor, and a use thereof. The compound not only has excellent tyrosinase inhibition activity, but also has good physicochemical properties, can be used for a required population at very low concentrations, and has no melanocyte or skin toxicity.

The application belongs to the technical field of cell induction differentiation culture and cell culture medium preparation, and particularly relates to a cell culture medium and application thereof in inducing olfactory mucosa mesenchymal stromal cells to differentiate into melanocytes. The culture medium has clear components, and the components used are mature commercial components, which are convenient and easy to obtain. The olfactory mucosa mesenchymal stromal cells are induced to become melanocytes expressing tyrosinase and containing melanin granules, which has the advantages of simple induction method and autologous induction avoiding ethical problems. The olfactory mucosa mesenchymal stromal cells are separated from nasal mucosa tissue, the nasal mucosa tissue is convenient to obtain, the nasal cavity part has rich blood supply, the wound is small and heals rapidly, and the human body is not continuously damaged. The prepared melanocytes can be used as seed cells for treating vitiligo disease, and have wide prospects.

The invention discloses an induction method for directionally differentiating hESC-melanocytes based on a neural crest lineage, and belongs to the technical field of cell engineering and cytobiology. According to the induction method, a complex differentiation process of embryonic stem cells-ectoderm-neural ectoderm-neural crest cells is abandoned, and the neural crest cells are directly obtained from embryonic stem cells, so that the differentiation process is simplified; the differentiation efficiency and controllability of the neural crest cells are remarkably improved, the obtained melanocytes have higher tyrosinase activity and melanin synthesis capacity, and a new way is provided for treating leucoderma by adopting a melanocyte transplantation method.

An object of the present disclosure is to provide a toxicity test system that does not use an animal and can determine the toxicity of rhododenol having a relatively weak white spot formation ability.SOLUTION: It has been found that a melanocyte state in which cytotoxicity is easily detected can be created by adding a melanization-inducing factor to a medium. By utilizing this finding, a culture toxicity test system containing melanocytes capable of detecting the toxicity of rhododenol was established.SELECTED DRAWING: Figure 1

The invention relates to application of Ro5-4864 or pharmaceutically acceptable salt in preparation of a medicine for treating and / or preventing melanocyte degeneration diseases caused by inflammatory immune response induced by oxidative stress. The melanocyte degeneration diseases caused by inflammatory immune response induced by oxidative stress comprise vitiligo, leukoplakia and pityriasis albicans. The Ro5-4864 and the pharmaceutically acceptable salt of the Ro5-4864, disclosed by the invention, inhibit continuous loss of melanocytes from oxidative stress, are strong in targeting effect and relatively small in side effect, and have remarkable advantages and progress compared with existing medicines.

This invention belongs to the field of cosmetic technology, specifically relating to a composition with water-locking and brightening functions and its targeted microcarrier delivery system. The water-locking and brightening composition provided by this invention mainly consists of niacinamide, tranexamic acid, oat β-glucan, disodium EDTA, and sodium hyaluronate. This composition can achieve a synergistic effect of water locking and brightening, effectively improving dullness, dryness, and uneven skin tone. Simultaneously, this invention also provides a targeted microcarrier delivery system, which is prepared by encapsulating the above composition in an internal aqueous phase using liposome technology, and simultaneously covalently attaching a targeting ligand to the surface of the liposomes. The targeted microcarrier delivery system obtained by this invention can actively target melanocytes and keratinocytes, achieving precise delivery of brightening and water-locking ingredients, significantly improving skin tone and addressing the root causes of dullness and uneven skin tone.

The present application belongs to the field of cell and tissue culture, and particularly relates to a culture medium for promoting proliferation of primary human epidermal melanocytes, a preparation method and application thereof. The present application innovatively uses epidermal concentrate factor to promote proliferation of melanocytes. The epidermal concentrate factor is obtained by collecting and concentrating the culture medium of keratinocytes. By simulating the in-vivo microenvironment, the epidermal concentrate factor prepared by the present application can effectively activate the proliferation signal pathway of melanocytes. The concentrate factor contains various growth factors and cytokines secreted by keratinocytes, which can synergistically enhance the division ability of melanocytes. Compared with the traditional culture method, after adding the epidermal concentrate factor, the population doubling time of primary melanocytes is significantly shortened, and the stable pigment production function is maintained.

Use of a carboxymethylcysteine compound or a hair care composition comprising a carboxymethylcysteine compound for increasing melanin production and / or for increasing cellular metabolic activity of melanocytes.

The purpose of the present invention is to develop cosmetics based on a novel mechanism of whitening action. It is known that melanocytes express olfactory receptors, and melanogenesis is suppressed when an aroma component capable of stimulating the expressed olfactory receptors is applied to the melanocytes. According to these cognizations, a cosmetic composition for whitening that acts via the activation of olfactory receptors is provided.

The application discloses a composition with whitening effect, which comprises the following components in mass fraction: 47-51 parts of grape seed oil, 47-51 parts of pomegranate seed oil, 0.5-0.8 parts of 4-butyl resorcinol, 0.2-0.6 parts of VC-IP and 0.1-10 parts of isorhamnetin-3-O-neohesperidoside. The application further discloses a preparation method of the composition. The application further discloses an essence oil containing the composition, and a cream containing the composition. The application further discloses application of the composition in preparation of cosmetics for skin whitening, freckle lightening and skin yellowing reduction. The composition establishes multiple target point synergistic pathways: one is a black removal pathway, which reduces activation of melanocytes under oxidative stress such as ultraviolet rays; and the other is a yellow removal pathway, which inhibits lipid peroxidation and promotes lipofuscin clearance.

The application discloses a kind of even skin tone whitening composition and its preparation method and application, and the even skin tone whitening composition includes: zephyranthes grandiflora extract, north American pinus strobus bark extract, sea freesia extract, synergistic effect exists between composition components, whole-path whitening, improve the efficacy of active substance.The composition inhibits tyrosinase activity, inhibits melanosome transfer to keratinocyte, inhibits melanin release to keratinocyte in this path, and also decomposes melanin into amino acid that can be used by human body by melanocyte autophagy to even skin tone, whitening skin.

The present application belongs to the technical field of cosmetics, and particularly relates to a recombinant humanized collagen composition, a preparation method and application. The present application first reports a recombinant humanized collagen composition, which is based on the independently developed recombinant humanized collagen, and through compounding chitosan, ectoine and other components, the application scene of the recombinant type IV collagen is effectively expanded; and the composition itself can reduce the ability of melanocytes to synthesize melanin, has the ability to scavenge free radicals, and can also reduce the amount of preservatives in the composition, effectively maintain the stability of the recombinant type IV collagen. The recombinant humanized collagen composition can be used for daily skin care, and can also help the recovery after medical and aesthetic surgery, and therefore has good practical application value.