7 results about "Ormosia" patented technology

The invention discloses a high-pressure propagation method for a branch of ormosia boluensis. The high-pressure propagation method comprises the following steps of: selecting an ormosia boluensis branch; belongs to the technical field of endangered plant asexual breeding. In order to solve the technical problems of extremely low survival rate (only 1-2 plants) and the like of the existing grafting, cuttage and other methods, a branch high-pressure propagation technology is successfully applied to the ormosia boularis for the first time, and nutrient soil is adopted, and a rooting solution and carbendazim are matched to serve as a rooting substrate; the girdling branches are wrapped with a high-pressure box, strict humidity control is carried out, and regular maintenance is carried out to induce rooting; during separation transplanting, the high-pressure soil wrapping ball is completely reserved to avoid damage to the root system; trimming and original habitat shading maintenance are assisted after transplanting. According to the method, the survival rate of the seedlings is successfully increased to 87.5%, vegetative propagation individuals of the boleo beans can be rapidly and efficiently obtained, and reliable technical support is provided for solving natural renewal obstacles of the boleo beans and achieving population rejuvenation and near depreciation of the boleo beans.

This invention discloses a grafting method for small-leaved red bean branches. It uses small-leaved red bean (… Ormosia microphylla ) as the scion, with red bean tree ( Ormosia hosiei This invention uses a grafting method that successfully expands the population size of *Ormosia henryi*, promoting its regeneration and rejuvenation. This invention is of great significance for the artificial assistance in de-emerging endangered plants.

The application relates to the technical field of seed seedling raising, and provides a seedling raising method suitable for seeds of the genus Ormosia and application thereof, the seedling raising method comprises the following steps: (1) in early March, the seeds of the genus Ormosia are put into hot water with a temperature of 60-80 DEG C, the water is naturally cooled, the seeds are taken out after being absorbed, and the seeds are sprayed with water to keep moist; (2) step (1) is repeated once a day, and the process is continued for 5-7 days, so that the seeds are obtained after preliminary treatment; (3) the seeds after preliminary treatment are soaked in a suspension seed coating agent for 10-18 hours, so that the seeds after soaking are obtained; (4) the seeds after soaking are inoculated into a substrate to accelerate germination for 30-50 days. The seedling raising method can improve the germination rate and germination uniformity of the seeds of the genus Ormosia, and simultaneously improve the quality of seedlings, increase the height and the number of lateral roots of the seedlings. The seedling raising method is low in cost, wide in applicability, easy to popularize, and can provide reliable technical support for seedling raising of the seeds of the genus Ormosia of the legume family.

The invention discloses a tissue culture method of ormosia arborescens, and relates to the technical field of tissue culture of ormosia arborescens, and the specific tissue culture process is as follows: selecting tender seedling stem segments with buds of ormosia arborescens as explants, disinfecting and sterilizing with ethanol and mercuric chloride, and performing induction culture, multiplication culture and rooting culture to obtain rooted seedlings; rooting seedlings are transplanted into a matrix for continuous growth after seedling hardening, so that the problems of low multiplication coefficient and rooting difficulty in the tissue culture process of the ormosia arborescens are solved.

The invention relates to the technical field of seed seedling raising, and provides a seedling raising method suitable for ormosia seeds and application thereof.The seedling raising method comprises the following steps that 1, the ormosia seeds are put into hot water at the temperature of 60-80 DEG C in the first ten days of March, the water is naturally cooled, the ormosia seeds are fished out after imbibition, and water is sprayed to the seeds to keep the seeds wet; (2) repeating the step (1) once every day for 5-7 days to obtain primarily treated seeds; (3) soaking the primarily treated seeds in a suspended seed coating agent for 10-18 hours to obtain soaked seeds; and (4) inoculating the soaked seeds into a substrate, and accelerating germination for 30-50 days. According to the seedling raising method, the germination rate and germination uniformity of the ormosia seeds can be improved, meanwhile, the bud seedling quality is improved, and the bud seedling height and the lateral root number are increased. The seedling raising method is low in cost, wide in applicability and easy to popularize, and reliable technical support can be provided for seedling raising of seeds of rare and valuable leguminous ormosia trees.

The application provides a method for simultaneously determining five active ingredients in plants of the genus Ormosia, and relates to the field of component identification technology.The method provided by the application comprises the following steps: respectively gradient diluting genistein solution, rutin solution, resveratrol solution, luteolin solution and kaempferol solution, establishing a standard curve of concentration and peak area after liquid chromatography analysis; gradient diluting a mixed solution containing genistein, rutin, resveratrol, luteolin and kaempferol, establishing a corresponding relationship between active ingredients and retention time after liquid chromatography analysis; obtaining the peak area of active ingredients in the test solution from the plants of the genus Ormosia based on the corresponding relationship after liquid chromatography analysis of the test solution, and calculating the content based on the peak area and the standard curve. The application can quickly explore the content difference of active ingredients in the plants of the genus Ormosia by establishing a liquid chromatography analysis method, and can provide theoretical guidance for the collection of the plants of the genus Ormosia and the control of planting environment.

The invention belongs to the technical field of plant tissue culture, and particularly relates to a construction method of a sainfoin callus induction regeneration system, which comprises the following steps: preparing sterile seedlings of sainfoin; cotyledons of the sterile seedlings are taken, wounds are formed at the tops of the cotyledons to prepare explants, and the explants are induced to form calluses; inoculating the callus to a differential medium for induced differentiation to form buds; performing regeneration culture on the bud points to obtain regenerated seedlings; and inoculating the regenerated seedlings on a rooting culture medium for culture and rooting induction. According to the method, the sainfoin cotyledons are adopted as the explants, and compared with hypocotyls and other tissues which are complex in structure and high in lignification degree, the cotyledons are easier to conduct wound induction and hormone response, and dedifferentiation and starting of the regeneration process are promoted. Due to the selection of the explant, the method disclosed by the invention has obvious advantages in the aspects of simplicity and convenience in operation, controllability and adaptability.