275 results about "Taxus species" patented technology

A method for quickly reproducing Mandiya yew is based on the tissue culture principle plus the cuttage technique includes such steps as taking part of the organs of said yew, treating it by the invented "tonic G-P clone liquid", planting in sand bed, supplementing said "tonic G.P clone liquid" while management, and growing root in 20 days. Its advantages are high speed and survival rate and low cost.

The invention relates to the taxus media culture technology, which belongs to the sticking culture technology. The taxus media culture technology comprises branch selection, nutrient solution soaking and soil sticking culture. The branch selection technology obtains the easiest surviving sticking branch selection standard of the taxus media, the selected and stuck branch soaking nutrient solution recipe and the environment conditions of the sticking soil substrate are very suitable for the survival of the taxus media. When the technology of the invention is used for propagation, the survival rate higher than 90 percent can be reached.

The invention relates to the technical field of tree planting, in particular to an interplanting technique of Taxus yunnanensis and walnuts. The invention aims at providing a stereoscopic ecological cultivation method of the Taxus yunnanensis and the walnuts. Through the reasonable interplanting of the Taxus yunnanensis and the walnuts, in combination with a precise field management technique, walnut trees create a more suitable growth environment for the Taxus yunnanensis and the goals of improving the survival rate and the growth speed of the Taxus yunnanensis are achieved; and by changing the branch shapes of the walnut threes at proper time and regulating the illuminating conditions, the growth of the Taxus yunnanensis is prompted at an early stage and the content of taxol in the Taxus yunnanensis is improved at a harvesting stage; and at the same time, the Taxus yunnanensis can take a reverse supplementing effect to the walnuts through the insect propelling characteristic of the Taxus yunnanensis, the influences caused by injurious insects to the walnuts are reduced and the quality of the walnuts is improved. The Taxus yunnanensis and the walnuts supplement each other and the produced economic benefits per unit land are greatly improved.

The invention provides a method for breeding and cultivating Taxus chinensis var mairei, and aims at improving the survival rate of Taxus chinensis var mairei bred through asexual cuttage. According to the method, Taxus chinensis seedlings are asexually bred through cuttage, and then large seedlings of Taxus chinensis and sapling grove are obtained through transplanting and planting to enlarge the cultivated area of Taxus chinensis var mairei. By adopting the specific cultivation management measures of Taxus chinensis var mairei, the technology system for breeding and transplanting Taxus chinensis var mairei is established, and an approach for the artificial reproduction of Taxus chinensis var mairei is hewed out, so that large-scale industrial production of Taxus chinensis var mairei becomes possible, thereby satisfying the market demand for Taxus chinensis var mairei, and providing a good test system for the protection of endangered wild plant resources.

Methods for obtaining paclitaxel from plants containing paclitaxel are disclosed. Plant material is first obtained from plants containing paclitaxel. Paclitaxel is then extracted from the plant material. Subsequently, paclitaxel is separated from the paclitaxel extract using a series of column chromatography separation steps to obtain at least one fraction containing paclitaxel. The paclitaxel in a fraction that is obtained from the final chromatography step is crystallized. Using these methods, high purity paclitaxel may be efficiently obtained from plants containing paclitaxel.

The invention discloses a method for propagating Taxus madia nursery stocks. The method comprises the steps of (1) preparing a sterile fine-sand seed bed and setting up a shading shed; (2) mixing turf with perlite in the proportion of 6:4 to prepare matrix nutrition cups; (3) matrix nutrition cups are evenly spaced and placed on the seed bed and sterilized fully; (4) selecting branches of Taxus madia plants of three to four year olds in spring or in autumn for cuttage in nutrition cups; and (5) keeping the temperature of the seed bed to be within a range of 18-30 DEG C and the humidity to be 65-70%, spraying foliage fertilizer for the first time after 9-10 days, spraying the foliage fertilizer once every 7-10 days during the fast-growing period of nursery stocks, cutting roots by air or breaking roots through movement of nutrition cups, stopping spraying the foliage fertilizer when most of roots grow, and then hardening seedlings till the next spring to conduct large field transplantation. According to the method, propagated Taxus madia nursery stock roots are balanced, neat and robust, the rate of survival exceed 96%, the seedling rate exceed 95%, the survival rate of transplanting can achieve 98%, and industrialized production can be achieved.

The invention relates to a cuttage breeding method of taxus chinensis in northeast, comprising the following steps: selecting a one-year-old branch as a branch for cuttage; concealing the branch at ashade place, and carrying the branch to a destination after the branch is packaged by a plastic bag; retaining 1-2 leaves on the upper end of each nursery stock stalk when shearing a seedling, shearing the parts longer than 3cm of the retained leaves, and also shearing leaves inserted into the stalk positioned in the middle of a seedling bed; disinfecting a land before the cuttage so as to reducerhizoctonia solani kuhn, and carrying out the cuttage from the middle ten days of October to the first ten days of November or from February to March; using a light shielding net of 80 percent to shield light without directly shining with the sun during the daily management of the seedling; controlling the soil water content at 35-45 percent; and paying attention to the water drainage of the seedling in rainy days and intertillage weed control in ordinary days, and preventing and curing diseases of the seedling in time by chemical agents. By adopting the cuttage breeding method, the taxus chinensis in northeast has high survival rate, fast breeding cultivation and high disease resistance.

The invention pertains to agricultural planting technological field, in particular to a stereoscopic ecological cultivation method of taxus Media and cinnamomum camphora. The method comprises planting, field management, harvest and so on. Seedlings of the cinnamomum camphora with a height of 1.5-2m are chosen. Seedlings of the cinnamomum camphora and the taxus Media are planted at a south-north direction in a determinant. 3 rows of taxus are interplanted among each 2 rows of the cinnamomum camphora. The row spacing and planting distance of the taxus is 60cm-80cm, the row spacing between the taxus and the cinnamomum camphora is 1.0-1.2m and the planting distance of the cinnamomum camphora is 1.5-1.8m. The taxus Media is planted during the period from March to April, and the cinnamomum camphora can be planted at the same time, so as to form obvious difference in height with the seedlings of the taxus Media, thereby forming the stereoscopic planting mode. The cinnamomum camphora is transplanted or put into a plantlet market after 3 years of interplant, the whole trunk of the taxus or some of the leaves are harvested and used for extracting paclitaxel medicine. The invention can promote the survival rate and growth speed of the taxus Media so as to reduce the planting cost, thereby having better economic value.

The invention discloses a method for cuttage of taxus chinensis, which comprises the following steps of: cutting out a cuttage branch, and soaking the cuttage branch in chemical slurry to disinfect; carrying out cuttage on the cuttage branch which is soaked and disinfected onto a seedling bed to implement non-fertilizer breeding until epidermis on a cutting wound of the cuttage branch grows and heals; spraying a nutrient solution onto the seedling bed to implement fertilizer breeding, so as to promote growth of roots and leaves from the branch; and after 3-6 months, implementing normal nursery management or transplantation. According to the method for cuttage of the taxus chinensis, the chemical slurry which is used for soaking the cuttage branch is prepared by mixing yellow soil, a plant peel promoting agent, a rooting agent, a sterilizing agent and water; the chemical slurry coated on the cutting wound part of the cuttage branch can effectively prevent the water and the nutrition in the branch from evaporating or losing, and can disinfect the cutting wound part of the branch and promote healing and corrosion prevention of fresh skin of the wound, and therefore, the survival rate of the cuttage branch is further improved.

The invention discloses a taxus chinensis var mairei artificial propagation technology, and belongs to the scientific and technological field of rare plant protection. The taxus chinensis var mairei artificial propagation technology aims to solve the problems that taxus chinensis var mairei is complicated in growth characteristic, low in growth speed under natural conditions and poor in regeneration capacity. Through countless tests for many years, taxus chinensis var mairei artificial propagation key indicators are captured, the survival rate, the propagation speed and the growth speed of taxus chinensis var mairei seedlings are improved, and the taxus chinensis var mairei artificial propagation technology is made to reach the domestic advanced level, wherein a hard-branch mass production type cutting technology fills up a provincial blank.

Disclosed is a culture composition containing micro and macro-ingredients, vitamins and other supplements such as phytagel, sucrose, picloram. The medium is useful in the formation and development of callus lines of Taxus sp., and resulting in production of taxol in high amounts.

The invention relates to a method for extracting paclitaxel from taxus chinensis branches and leaves, and the method comprises the following steps of: (1) soaking 1000 kg of pulverized taxus chinensis branches and leaves by using 5 m<3> of methanol, and filtering; (2) decoloring filter liquor; (3) decompressing and concentrating a decolored solution, and extracting; (4) degreasing and dewaxing extract liquor, crystallizing, depositing, filtering, and drying to obtain a primary product; (5) removing impurities contained in the primary product in a silicagel column, carrying out gradient elution, collecting paclitaxel-containing elution flows, and decompressing and concentrating to obtain a crude product; (6) stirring the crude product into silica gel, packing, carrying out gradient elution, collecting the paclitaxel-containing elution flows, and decompressing and concentrating to obtain a semi-finished product; (7) dissolving the semi-finished product, adding petroleum ether, standing, filtering, and drying to obtain a semi-finished product with the content more than or equal to 30%; (8) removing the impurities contained in the semi-finished product obtained from the step (7) in a silicagel column, carrying out gradient elution, collecting the paclitaxel-containing elution flows, and decompressing and concentrating to obtain a semi-finished product with the content more than or equal to 95%; and (9) dissolving the semi-finished product obtained from the step (8), filtering, adding normal hexane, standing, carrying out suction filtration; and drying to obtain a finished product with the content more than or equal to 99%.

The invention relates to a cutting and breeding method for taxus chinensis, and belongs to the technical field of plant breeding. The cutting and breeding method comprises the following steps: scion wood selection, scion wood treatment, scion wood cutting, management after cutting, seedling transplantation, and management after transplantation. The adopted cutting and breeding method has the advantages that the cutting and breeding method with high survival rate is provided for taxus chinensis breeding in the south, the planting range of taxus chinensis is enlarged, and the difficult problem of taxus chinensis resource shortage can be favorably solved.

The invention belongs to the technical field of cultivation of taxus mairei, and in particular relates to a method of growing seedlings of taxus mairei. The method comprises the steps of: harvesting and storing seeds, selecting and treating a garden, sowing, exposing grass and shading, managing seedling, carrying out pest control, outplanting and grading nursery stock. According to the method of growing seedlings of taxus mairei provided by the invention, by adopting seeds subjected to sand storage, humidification and natural temperature stratification, the integrated germination rate is more than 95%. Through reasonably designing a process for exposing grass and shading, managing seedling, and carrying out pest control, the cost for growing the seedlings of the taxus mairei is reduced, and the integrated survival rate is increased.

The invention relates to an extraction method of taxol from branches and leaves of artificially cultivated yew. A plurality of methods, such as planting of stocky yew, cultivating of tissue and semi-synthesis and total synthesis of taxol, have been developed home and abroad so as to obtain taxol raw material; however, the tissue cultivating method has low yield and high broth price; and the total synthesis has extremely low yield, worse therapeutic effect than natural-source taxol, and a high cost; thereby being difficult for commercial application. The invention uses branches and leaves of artificially cultivated taxus media, Yunnan taxus, southern taxus or northeastern taxus as raw materials; the raw materials are marinated by carbinol and extracted by petroleum oil and propyl acetate in sequence; and normal-pressure normal-phase silica gel column chromatography or reverse-phase high-pressure liquid chromatography is used to obtain high-purity taxol with a purity being higher than 99.6%. The preparation method has simple process, low cost, recyclable solvent and eluant; therefore, the method is applicable to industrial production and protects plant resource.

The invention provides a taxus chinensis propagating method. The method is characterized by comprising the following steps that 1, taxus chinensis branches are selected; 2, bactericide is used for treating the branches; 3, a plant growth regulator is used for treating the branches; 4, water culture is adopted for inducing the branches to take root; 5, a nutrient solution is added for curing. By the adoption of the method for propagating taxus chinensis, restriction of cultivated land resources can be gotten rid of, and the propagation area is increased; meanwhile, the influence on the survival rate from external environment is reduced to the maximum, time for callus forming and rooting is shortened, the stem cutting survival rate is remarkably increased, the restriction that due to poor environmental factors such as temperature, humidity, soil and water quality, the stem cutting survival rate is influenced by an original propagation means is broken through, the current situation that taxus chinensis genetic resources are scanty can be relieved, and great economic value can be created.

The invention relates to a Taxus media cultivation method which comprises the following steps: (1) preparing before planting, (2) planting tree seedlings and (3) field management. The step (1) includes the following steps: selecting a cultivation field, and selecting cultivation seedlings. The step (2) includes the following steps: in March or April, planting the cultivation seedlings into the cultivation field with the row spacing and line spacing respectively of 55-65 cm, enabling soil moisture content in the cultivation field to be 30%-40%, and ensuring survival of cultivation seedlings to form small trees. The step (3) includes the following steps: weeding; conducting one-time topdressing to all small trees in April or May each year from the next year when planting is completed, concretely, topdressing bio-organic fertilizer 90-100 g, and topdressing compound fertilizer 50-60 g; spraying plant growth regulators to all small trees in April or May each year from the next year when planting is completed; and collecting new branches and leaves of the small threes in October or November each year from the next year when planting is completed, wherein the new branches and leaves are grown in the current year. By means of the Taxus media cultivation method, the cultivation seedlings can reliably survive, fast grow, and stably accumulate valid biomass.

The invention discloses a grafting and cultivation method of Taxus chinensis, and belongs to the technical field of traditional Chinese medicine material planting. The method includes preparation before grafting, grafting operation and management after grafting. The preparation before grafting, comprises the steps that grafting time is June to July, a scion is collected 15-30 d before grafting, the whole scion is sealed with paraffin and is put into wet sand in a cool place for preservation. The rafting operation comprises the steps of a, treatment of cutting scion, wherein the grafting opening is dipped in a root growing foliar fertilizer for standby application; b, grafting, wherein stopping cutting at the grafting part, one side of a rootstock section is cut into a concave triangular rootstock groove using a grafting knife, the scion is taken out, the other end of the rootstock section is cut into a convex triangle corresponding the concave triangle rootstock groove, the scion is embedded into the rootstock groove, both sides of the rootstock groove are made to be adjoined, and a callus anti-corrosion film is smeared. The management after grafting comprises the steps of a, unbinding and removing burgeons; b, applying the fertilizer and moisture management; c, grafted seedling pest control. According to the grafting and cultivation method of Taxus chinensis, grafting is conducted in June to July, the method is suitable for grafting of a big rootstock, and has the advantages of being fast in shooting, early in formation of crowns, smooth in grafting healing part and high in grafting survival rate.

The invention relates to a Chinese yew seedling cultivation technology which comprises the steps of: retting seeds, washing flesh and peeling off, removing wax coat; disinfecting; drying in the sun; storing in a big stone mortar with holes for 12 months; when the seeds crack and expose white parts, dipping the seeds in 0.05% potassium permanganate liquid for disinfecting; washing with clean water; drying in the air and then seeding after the beginning of winter and before the beginning of spring; after seedlings come out, applying 800 multiple thiophanate to stems, leaf backs and leaf surfaces of the seedlings; sun shading; outplanting when the seedlings are 20-30 cm in height, and have ground diameter of 0.3cm and 3-5 lateral branches. The Chinese yew seedling cultivation technology has the advantages of high seedling yield, strong seedling, fast growth and early fruiting; in addition, space among Chinese yew seedlings can be used to plant soybeans to increase grain income, moreover, roots of soybeans can increase biological nitrogen and improve soil for the rooting of Chinese yew, and the soybean seedlings can shade the Chinese yew seedlings for resisting high temperature, thereby improving the survival rate of the Chinese yew seedlings. The invention combines agriculture and forestry, develops ecological agriculture, and achieves both agriculture benefits and forestry benefits.

Disclosed is a method of and apparatus for manufacture of a natural antimicrobial, in which a first mixed solution is created by mixing Taxus extract, Thymus extract and ethanol; a second mixed solution is created by mixing oleic acid with the first mixed solution; a third mixed solution is created by mixing phytoncide with the second mixed solution; and a fourth mixed solution is created by mixing polysorbate with the third mixed solution, and the natural antimicrobial is completed by mixing ethanol, emulsification and water with the fourth mixed solution.

The invention relates to a method for simultaneously extracting taxol and 10-DABIII (10-deacetyl baccatine III) from branches and leaves of Taxus media, which separately employs ultrasonic extraction, solvent lixiviation and Soxhlet extraction and comprises the following steps: concentrating an extracted methanol solution to be dry while depressurizing, extracting with n-hexane to remove pigment and other fat-soluble impurities, extracting with dichloromethane to obtain a final extraction liquid of taxol and 10-DABIII, and detecting the contents by HPLC (high-performance liquid chromatography). The method provided by the invention fully takes advantages of the different extraction methods to simultaneously extract taxol and 10-DABIII, improves the utilization rate of resources, and has the characteristics of simplicity, rapid extraction and high efficiency.

The invention relates to a method for using specific resin and silicon gel column chromatography purification via negative pressure cavitation water extraction to high-efficiency produce 10-deacetylbaccatin III and 7-xylose-10-deacetylpaclitaxel, which uses taxus branch leaves as raw material, dries and breaks to process negative pressure cavitation water extraction, filters and uses AB-8 resin to process dynamic absorption on filter liquor to concentrate 10-deacetylbaccatin III and 7-xylose-10-deacetylpaclitaxel and process further purification via middle pressure silicon gel column chromatography, to obtain the 10-deacetylbaccatin III of at least 60% purity and 7-xylose-10-deacetylpaclitaxel of at least 67% purity. The invention can use renewable taxus branch leaves to protect ecological resource and effectively extract effective component and biological semi synthesis precursor materials, with simple operation, support for industrial application and important industrial production value.

The invention relates to the technical field of interplanting, in particular to an interplanting method for photinia fraseri and Chinese yew. Through interplanting the photinia fraseri and Chinese yew, the beauty of the Chinese yew in autumn and winter and the beauty of the photinia fraseri in spring, summer and autumn compensate for each other, a planting base locates at an ornamental stage throughout the year, and the ornamental value of the planting base is improved; through reasonably interplanting the photinia fraseri and Chinese yew, the Chinese yew and photinia fraseri act upon each other, and then the plant diseases and insect pests on the Chinese yew and photinia fraseri in the planting process are lowered; by means of the interplanting mode, the Chinese yew planting base is used in a large area, and then the vacant area of the Chinese yew base is lowered; the photinia fraseri plant is produced in the Chinese yew planting process, and then the Chinese yew planting profit is improved.

The invention relates to a cultivation and collection method for improving taxol content in taxus media branches and leaves, belonging to the technical field of medicinal plant cultivation. One-year-old or more than one-year-old taxus medias are cultivated in optimized soil with pH of 6.0 to 6.9 under sunny natural daylight; proper nitrogenous fertilizer, phosphate fertilizer and potash fertilizer are applied around summer begins and autumn begins each year, and newly growing branches and leaves in the current year are picked around winter begins, are dried in the shade or dried at the low temperature of 45 DEG C. According to the method provided by the invention, taxol content in taxus media branches and leaves can be improved by about 10 to 17%, and the quantity of branches of each taxus media is improved, thus effectively relieving shortage of medicine resource of taxus media, lowering the cost of medicine taxus media, and producing great economic benefit and social benefit.