88results about How to "Increase the cultivation area" patented technology

The invention belongs to the field of plant detoxification tissue culture rapid propagation technology, and mainly relates to a strawberry detoxification tissue culture method under LED condition, which comprises the steps of: selection of culture conditions, preparation of a culture medium, selection and sterilization of an explant, induction culture, propagation culture, strong seedling culture, rooting culture and transplant. The strawberry detoxification tissue culture method has the advantages that an LED is used as a tissue culture light source, the effects of strawberry induction, propagation, strong seedling and rooting culture and the tissue culture seedling quality are not different from those of a fluorescent lamp, or the culturing effect is better than that of the fluorescent lamp; the survival rate of the tissue culture seedlings is higher than 90%, the culture area is enlarged by 40% compared with the area when the fluorescent lamp is used, and the electricity is saved by 30-50%; and the cost is reduced, and the efficiency of strawberry detoxification tissue culture factory production can be improved.

The invention discloses a potted plant substrate bag and belongs to the technical field of plant container culture and substrate culture. In order to solve various troubles and the pollution problem occurring during potting, pot change, soil utilization, watering and management of household potted plants at present, the invention provides a household material which is clean, easy, fast and happy for performing the container culture. The technical scheme disclosed by the invention is that the potted plant substrate bag is manufactured by wrapping a potted plant substrate with permeable and breathable cloth, and is characterized in that the substrate is completely wrapped by the cloth; the substrate bag is put in a potted plant container to perform the container culture; the substrate bag is soft and is deformable; the planting side of the substrate bag can be molded, and even can be made into a three-dimensional modeling and a cartoon modeling. Three-dimensional culture can be performed on the three-dimensional modeling; and a waterproof film also can cover on the planting side of the substrate bag. The potted plant substrate bag has functions of various functional mulching films. The invention also provides a culture and application method for the potted plant substrate bag.

The invention provides a method for breeding and cultivating Taxus chinensis var mairei, and aims at improving the survival rate of Taxus chinensis var mairei bred through asexual cuttage. According to the method, Taxus chinensis seedlings are asexually bred through cuttage, and then large seedlings of Taxus chinensis and sapling grove are obtained through transplanting and planting to enlarge the cultivated area of Taxus chinensis var mairei. By adopting the specific cultivation management measures of Taxus chinensis var mairei, the technology system for breeding and transplanting Taxus chinensis var mairei is established, and an approach for the artificial reproduction of Taxus chinensis var mairei is hewed out, so that large-scale industrial production of Taxus chinensis var mairei becomes possible, thereby satisfying the market demand for Taxus chinensis var mairei, and providing a good test system for the protection of endangered wild plant resources.

The invention discloses a nutrient solution for grafting of red kiwi and a use method of the nutrient solution. The nutrient solution comprises an I type nutrient solution and an II type nutrient solution, wherein the I type nutrient solution contains the following constituents: 1,320mg ammonium nitrate, 1,150mg nitrate, 350mg calcium chloride, 240mg magnesium sulfate, 125mg potassium dihydrogen phosphate, 32.5mg sodium ethylene diamine tetracetate, 20.3mg ferrous sulfate, 4.3mg boric acid, 0.35mg potassium iodide, 15.7mg manganese sulfate, 0.02mg copper sulfate, and 7,200mg saccharum or agar that are dissolved in every 1L water, and the II type nutrient solution contains the following constituents: 0.35mg naphthylacetic acid and 0.35mg indolebutyric acid that are dissolved in every 1L water. The nutrient solution for the grafting of the red kiwi can prolong the effective to-be-grafted time of sprouting to be grafted, increase the survival ratio and utilization ratio of the grafting and facilitate the production and popularization of kiwi planting.

The invention provides a large scale production technology for a dental pulp stem cell. The large scale production technology comprises following steps of (1) separating and extracting the dental pulp stem cell; (2) amplifying the dental pulp stem cell through two dimension cultivation; (3) detecting the dental pulp stem cell; (4) cultivating the dental pulp stem cell on a large scale; (5) freeze preserving the dental pulp stem cell; and (6) freeze preserving the dental pulp stem cell for a long term. The invention provides an in vitro large-scale cultivating and amplifying method for a dental pulp stem cell, establishes a long-term, standard and systematized freeze preserving system for a high quality high activity dental pulp stem cell, so that the method can provide a high enough quality stem cell resource for clinic and scientific researches, and has low cost and a wide application prospect.

A "Jiuxiang" strawberry stem tip rapid breeding method by tissue culture and virus removal is disclosed. The rapid breeding method includes: explant disinfection, stem tip tissue virus removal and culture, multiplication culture, rooting culture, seedling hardening and transplanting. A stem tip tissue culture method is adopted to breed "Jiuxiang" strawberry virus-free seedlings, thus ensuring that all the excellent characteristics of parents are stably inherited, and therefore influences of outside conditions can be prevented, and the rapid breeding method can be performed at all seasons, thus saving the seedling culture land, and reducing the production cost. A large quantity of good test-tube plantlets can be produced in a short period of time. Large-scale production can be performed to provide "Jiuxiang" strawberry seedlings with a large quantity and good quality for the market so as to meet market needs. The "Jiuxiang" strawberry virus-free seedlings prepared by the rapid breeding method are high in growth vigor, disease-resistant and increased in big-fruit yield. The yield can be increased by 30-50%.

The stereo sunny plantation may be set on water surface, ground, roof, etc. and has one sun following turntable, on which there are several rows of culturing shelves with horizontal culturing grooves holding nutritive liquid and floating culture medium for plant to grow. On the culturing shelves, there may be trays or hung pots for various varieties of plant to grow. On the turntable, which may be as small as one square meter and as large as one hectare, there is solar energy greenhouse. The present invention may increase the culture area and raise the culture efficiency for several times.

The invention provides a method for breeding and cultivating taxus mairei with a seed, and the method comprises the following steps that a taxus mairei seedling is obtained by breeding and cultivating the sexual seed; the taxus mairei seedling is transplanted and planted, and a large taxus mairei seedling and a young tree are obtained; and the cultivation area of the taxus mairei is expanded. Special cultivation management measures for the taxus mairei are adopted to establish a taxus mairei breeding and cultivation technical system, a manual way to breed the taxus mairei is opened up, so that the large-scale industrial production of the taxus mairei becomes possible, the market demand for the taxus mairei is met, and a good test system is provided for protecting endangered species of wild plant resources.

The invention provides a scale production process of adipose-derived stem cells. The production process comprises the following steps: (1) separating and extracting adipose-derived stem cells; (2) culturing and amplifying the adipose-derived stem cells; (3) detecting the adipose-derived stem cells; (4) culturing the adipose-derived stem cells on a large scale; (5) refrigerating the adipose-derived stem cells for preserving; (6) preserving the adipose-derived stem cells for a long time in a refrigerating manner. An in-vitro scale culturing and amplifying method for adipose-derived stem cells is provided, and a long-term, standard and systemic refrigeration preserving system is established for high-quality and high-activity adipose-derived stem cells, so that sufficient high-quality adipose-derived stem cell resources are provided for clinical treatment and scientific research. The process is low in cost and wide in application prospect.

The invention relates to the field of biotechnology, in particular to a virus seed for producing vaccines for preventing human rabies by utilizing human diploid cells (KMB17) and a preparation method thereof. In the invention, a rabies fixed virus CTN-1V5 strain is continuously subcultured in the human diploid cells (KMB17), and a terminal dilution method is used for screening viruses with highertiter, thereby obtaining a rabies virus strain which is suitable for the human diploid cells (KMB17) and has good immunogenicity and heredity stability, and culturing a rabies vaccine virus seed (CTN-DK strain) capable of efficiently reproducing in the human diploid cells (KMB17). By using the virus seed for producing a human diploid cell (KMB17) rabies vaccine, the risk caused by residual heterogonous DNA (deoxyribonucleic acid) in the vaccine which is currently used at home can be effectively avoided, and the safety and practicability of the rabies vaccine in China are further improved, thus the invention has great social and economic benefits.

A road soil slope willow post protection technique comprises a slope protection and a bridge protection. The invention is characterized in that the slope protection comprises seed selection, ground furnish and mechanical posting; the bridge protection comprises seed selection, ground furnishing, drawing line and positioning, digging concaves, and planting the seed. The invention can be used to protect two side slopes of road.

The invention relates to an early-bearing and high-yield Camellia oleifera cultivation method and belongs to the technical field of economic forest planting. The method comprises steps as follows: (1) establishment of forest land: selection of nursery stocks, land preparation, application of base fertilizer and planting; (2) management of a young forest: application of fertilizer, tending and weeding as well as tree shape pruning; (3) disease and pest control: disease control and pest control; (4) transplantation. With the adoption of the Camellia oleifera cultivation method, 60 kg of camellia oil is averagely produced per mu in the fifth year after planting, compared with the traditional yield of 10-15 kg of the camellia oil per mu, the yield is increased by 3-5 times, and the method is an important early high-yield and high-efficiency technology for Camellia oleifera. A Camellia oleifera high-yield forest has an area of 23 mu, 61.25 kg of the camellia oil is averagely produced per mu in 2015, and 1408.75 kg of the camellia oil is produced totally.

The invention relates to the technical field of plant cultivation and specifically relates to a strawberry cultivation pattern. The strawberry cultivation pattern comprises shed frame construction, variety selection, planting management and planting post-management, wherein planting management comprises disinfection of a greenhouse, disinfection of a matrix, planting processing, and management in a planting date. The strawberry cultivation pattern has the advantages that the scientific controllable management is implemented, the space is efficiently utilized, water and a fertilizer are saved, meanwhile, the yield of a strawberry is high, and the quality of strawberry is good.

The invention relates to a camellia oleifera high-yield cultivation technical method and belongs to the technical field of camellia oleifera cultivation and planting. The method includes the following steps of plantation land selection, soil preparation, afforestation, cultivation management and disease and insect pest prevention. According to the camellia oleifera cultivation method, 80 kg of camellia oleifera is produced per mu in the fourth year after planting. Compared with the mode that 10-15 kg of camellia oleifera is produced per mu in traditional forest, the yield is increased by 4-6 times, and the method is an important camellia oleifera high-yield efficient technology. The area of camellia oleifera high-yield plantation is 12 mu, the average yield per mu of camellia oleifera is 80.25 kg in 2015, and the total camellia oleifera yield is 963 kg.

The invention discloses a method using the tissue culture seedlings of red-pulp apples to produce anthocyanin. The method includes the steps of firstly, acquiring sterile materials; secondly, performing primary culture to obtain the tissue culture seedlings of the red-pulp apples; thirdly, performing subculture to obtain a large amount of tissue culture seedlings of the red-pulp apples; fourthly, culturing strong seedlings; fifthly, extracting the anthocyanin and preparing crude products. Experiments prove that the anthocyanin content of the tissue culture seedlings, cultured by the method, of the red-pulp apples can reach up to 359.86U.g<-1>FW, is 7.8 times of that of conventional sucrose media under room temperature and is respectively 1.8 times, 2.8 times, 2.9 times and 3.6 times of those of the pericarp 12 weeks after anthesis, new leaves, pulp 12 weeks after anthesis and flowers of the fruiting 5-year-old red-pulp apple trees. The method has the advantages that the method is not limited by time and raw material shortage, the industrial production of the anthocyanin is achieved by using the tissue culture method, and a new approach is provided for the production of the anthocyanin.

The invention discloses a three-dimensional pipeline intelligent control soilless culture system, which comprises a liquid storage pipe (1) sealed by a sealing cover, a liquid return pipe (5), a main pipeline bracket (13), a main liquid inlet pipe (17), a culture pipe (6) with planting holes, liquid level adjusting water outlet seal heads (22), an upper liquid outlet pipe (16), a lower liquid inlet pipe (15), a circulating water pump (10), an intelligent automatic controller (18), a standby liquid charging cup (26), a first concentrate preparation cup (3), a second concentrate preparation cup (3), a water inlet joint electromagnetic valve (7), a water level detector (19), a main pipeline bracket connecting port (25), a liquid supply pipe (12) and the like, wherein the liquid level adjusting water outlet seal heads (22) are arranged at the two ends of the culture pipe (6); and the standby liquid charging cup (26) is arranged on the top of the liquid storage pipe (1). The three-dimensional pipeline intelligent control soilless culture system has the advantages of rational structure, flexibility and convenience in assembly and disassembly, high automation degree, water saving, power saving, low use cost, full nutrient absorption by vegetables, high growth speed, small floor area and large culture area, and is suitable for being used in families, hotels, exhibition halls and the like.

The invention discloses a virus-free tissue culture and rapid propagation method of Olea europaea. The method comprises the following steps: 1, removing seed shells: removing seed shells through a sanding technology; 2, making explants: immersing the shelled seeds in a sucrose solution for 3-5h, taking out the immersed seeds, drying the immersed seeds in a dark place, and disinfecting the dried seeds; 3, carrying out inductive culture: inoculating the explants to an inductive culture medium to form callus tissues, transferring the callus tissues to a differentiation culture medium, and inducing clumpy buds to form rootless tissue seedlings; 4, carrying out propagation culture: inoculating the rootless tissue seedlings to a propagation medium, and carrying out subculture; 5, carrying out rooting culture: inoculating the rootless tissue culture seedlings to a rooting culture medium, and carrying out rooting culture; 6, carrying out seedling strengthening culture: carrying out seedling strengthening culture on the rooted tissue culture seedlings in a seedling strengthening culture medium for 10d; and 7, transplanting: tansplanting the seedlings after the virus-free tissue culture seedlings grow to 3-5cm, and culturing the transplanted seedlings to form young plants. The method is a new method for growing Olea europaea seedlings, accelerates the seedling growing speed, and meets scale production requirements.

The invention relates to the technical field of boletus aereus culture and particularly relates to a boletus aereus culture medium, a preparation method therefor and an application of the boletus aereus culture medium. The boletus aereus culture medium provided by the invention is prepared from wood shavings, straw, red soil, cereal grains, rice bran, gypsum powder, a minor-element additive and ayeast extract, wherein the wood shavings is one or a mixture of at least two of Chinese chestnut wood, Cyclobalanopsis glauca wood, alder and Betula luminifera wood. The limitation that materials of existing boletus aereus culture media are restricted to growth cycle, regions or culture use is broken through, the region of culture is enlarged, and meanwhile, the raw materials are wide in source, large in quantity and low in cost; and the preparation method is simple, and the production cycle is short. By adopting the boletus aereus culture medium provided by the invention, the growth speed ofmycelia of boletus aereus can be accelerated, the boletus aereus culture time is shortened, meanwhile, the boletus aereus yield, average boletus aereus weight and boletus aereus quality are improved,thus, the productivity is greatly increased, the production cost is reduced, and the boletus aereus culture medium has remarkable economic benefit.

The invention relates to a culture method of photosynthetic microorganisms. According to the method, photosynthetic microorganisms are inoculated into a thin-layer liquid medium to be cultured, wherein thickness of the culture solution is not more than 10 cm; any homogenization operation is not carried out on the culture solution within the culture period; and a light source is provided for irradiation of the culture solution. A photobioreactor for culturing the photosynthetic microorganisms according to the above method is also included. The photobioreactor comprises at least a board body, which extends along the horizontal direction. The board body has opposite upper and lower surfaces, and the periphery is provided with a cofferdam part which extends upwards from the upper surface of the corresponding board body. The upper surface of each board body and the inner surface of the corresponding cofferdam part form a space for containing the culture solution for culture of the target photosynthetic microorganisms. The minimum vertical height of the cofferdam part is not more than 10 cm. The invention also relates to a photobioreactor containing multilayer of the board bodies which are stacked at certain interval in the vertical direction.

The invention relates to a method for high-yield cultivation of walnut and belongs to the technical field of walnut cultivation. The method comprises the steps of 1, planted land selection; 2, soil preparation; 3, afforestation; 4, culture management; 5, insect disease control. According to the method, the average yield of walnut oil per mu is 100 kg at the fourth year after field planting, yield is increased by 4-6 times compared with traditional forests whose yield per mu is 20-35 kg, and the method is a key technique for high-yield and efficient cultivation of walnut. By the adoption of the method, the area of a walnut high-yield forest is 15 mu, the average yield of walnut oil per mu is 80.25 kg in 2015, and total walnut oil yield is 1500 kg.

The invention provides a large-scale production process of uterine membrane stem cells. The process comprises the following steps: (1) performing separation extraction and primary culture on the uterine membrane stem cells; (2) performing culture amplification on the uterine membrane stem cells; (3) performing large-scale culture on the uterine membrane stem cells; (4) detecting the uterine membrane stem cells; (5) storing the uterine membrane stem cells in a freezing manner; and (6) storing the uterine membrane stem cells in the freezing manner for a long time and performing recovery detection at regular time. According to the process, the uterine membrane stem cells are obtained from the menstrual blood of a female at a child-bearing period, so that a large quantity of high-quality uterine membrane stem cells rich in functional activity can be obtained in short time by utilizing an in-vitro culture amplification method; and the uterine membrane stem cells are stored in the freezing manner for a long time under the environment of -196 DEG C after being detected to be qualified, thereby providing a large quantity of uterine membrane stem cell resources for clinical and scientific researches in the future. Thus, the great social value and significance is achieved.

The invention relates to a carrier device for conducting tensile stress loading on cells, and the device is composed of an upper layer clamping clamp, an intermediate layer clamping clamp, a lower layer clamping clamp, multiple screws, an upper layer silicone rubber membrane and a lower layer silicone rubber membrane. The area of a cell culture basement membrane is increased through the clamping clamps and the screws, meanwhile, double layer design is adopted, and the effective culture area of each stressed cell is greatly increased. By means of the double-layer assembly type cytomechanics loading carrier device, effective mechanical loading can be conducted on the four layers of cells (the front side and the reverse side of the basement membrane) at the same time, and the requirement of the high throughput test for the number of cells is satisfied. The clamps, the screws and the basement membrane are all made of transparent materials, and the biocompatibility is excellent. Real-time microscopical cell morphology observation can be achieved. The whole device is simple and fast in assembling process and cell inoculation process, after being assembled, the device is fitted to all types of existing mechanical loading equipment with axial tension as the design principle as a whole, and loading and unloading are both convenient and efficient. The whole carrier device can be sterilized once again and used repeatedly, the experimental process is simplified, and experiment efficiency is greatly improved.

The invention discloses a system for producing and preparing exosome and a method for preparing the exosome, and particularly relates to the field of biological medicine. The method comprises two parts of cell culture and exosome extraction. The system comprises a first storage tank, a second storage tank, a hollow fiber cell culture device, a tangential flow filtering device, a first peristaltic pump and a second peristaltic pump. The system can be applied to industrial large-scale production and preparation of the exosome. According to the system, a large number of hollow fiber tubes in the hollow fiber device are utilized, so that the cell culture area is increased; cells can be promoted to secrete a large amount of exosome through a culture medium conveyed by the hollow fiber tubes, and the produced exosome solution is purified through the tangential flow filtering device; the system is simple to operate, high in recovery rate, free of intermediate pollution, high in purity and high in concentration. By means of the system, the integrated preparation process from cell culture to exosome production can be realized, and the system is very suitable for industrial large-scale production and preparation of clinical-grade exosome.

The invention discloses a nutrient solution for grafting of red kiwi and a use method of the nutrient solution. The nutrient solution comprises an I type nutrient solution and an II type nutrient solution, wherein the I type nutrient solution contains the following constituents: 1,320mg ammonium nitrate, 1,150mg nitrate, 350mg calcium chloride, 240mg magnesium sulfate, 125mg potassium dihydrogen phosphate, 32.5mg sodium ethylene diamine tetracetate, 20.3mg ferrous sulfate, 4.3mg boric acid, 0.35mg potassium iodide, 15.7mg manganese sulfate, 0.02mg copper sulfate, and 7,200mg saccharum or agar that are dissolved in every 1L water, and the II type nutrient solution contains the following constituents: 0.35mg naphthylacetic acid and 0.35mg indolebutyric acid that are dissolved in every 1L water. The nutrient solution for the grafting of the red kiwi can prolong the effective to-be-grafted time of sprouting to be grafted, increase the survival ratio and utilization ratio of the grafting and facilitate the production and popularization of kiwi planting.

The invention discloses a modular biological incubator, which comprises a box-shaped housing which is formed by superposing several layers of units. The top surface of the box-shaped housing is a cover plate having two feed inlets. Each layer of units is composed of a base plate and a sidewall. A charging channel is arranged at the position corresponding to the two feed inlets in the box-shaped housing. The charging channel passes through the base plate of each layer of units. Outer walls of all the layers of units are provided with at least two fixing lugs containing screw holes. Positions of the fixing lugs at all the layers of units are corresponding to each other. Fastening screws pass through the screw holes. The modular biological incubator provided by the invention has a multilayer structure. Each layer of units can be individually replaced, and the number of the layers of units can be increased and decreased according to needs. The modular biological incubator is convenient and flexible to use and can be used to greatly increase microbial cultivation area and raise output.

The invention relates to a method for culturing rumex dentatus in sea water. The method comprises the following steps: selecting seeds having full and healthy grains without wizening or worm damage; soaking the seeds with warm water, soaking with a naphthylacetic acid solution, dressing the soaked seeds with a matrix having 80-100 meshes; establishing a seedbed from mudflat sludge, thoroughly watering 1-1.5 days before sowing, uniformly spreading dressed seeds onto the seedbed, and growing seedlings; selecting seedlings with excellent growth and no disease or insect pest after 35-40 days of sowing, planting on the mudflat sludge substrate, supplying sea water having the concentration of 0.5-0.7 percent, and irrigating with sea water having the concentration of 1.8-2.5 percent; and harvesting in the late December. The method is convenient and easily available, and is favorable to expanding the rumex dentatus mudflat culture area by adopting sea water irrigation; and the rumex dentatus has high survival rate and high content of rheum emodin.

The invention discloses a culture device for adherent cells, a 2.5D beehive type culture system and method. The culture device comprises a culture jar, a TC bracket and TC culture plates, wherein the TC bracket is arranged in the culture jar and is provided with a plurality of fixing parts which are arranged at different heights, and the fixing parts are used for mounting a plurality of TC culture plates in a layered manner; and the two surfaces of each TC culture plate are cell culture surfaces, and the TC culture plates are detachably arranged on the fixing parts with different heights respectively to form multiple layers of cell culture surfaces. The 2.5D beehive type culture system comprises the culture device and a mixture of an adherent cell culture solution and a macromolecular solute. According to the method, the mixture is injected into the 2.5D beehive type culture system for culture. The culture device provided by the invention is favorable for increasing the culture area of unit volume; the provided 2.5D beehive type culture system can simulate a 3D culture environment; and the method provided by the invention can be used for promoting cells to secrete more extracellular matrix proteins.

The invention provides a planting device of centella asiatica and cultivation methods thereof, which relates to the field of Chinese herbal medicine cultivation technology. The planting device comprises a base groove, a first planting groove and a second planting groove. The base groove comprises a planting base surface; the first planting groove and the second planting groove are arranged on the planting base surface. A groove plate of the first planting groove and a groove bottom plate of the second planting groove are arranged opposite to each other. One side of the first planting groove which is located away from the base groove and the side of the second planting groove which is remote from the base groove are close and spaced apart from each other, to form an exposed opening for exposing a planting base surface.

The invention relates to the field of biotechnology, in particular to a virus seed for producing vaccines for preventing human rabies by utilizing human diploid cells (KMB17) and a preparation method thereof. In the invention, a rabies fixed virus CTN-1V5 strain is continuously subcultured in the human diploid cells (KMB17), and a terminal dilution method is used for screening viruses with highertiter, thereby obtaining a rabies virus strain which is suitable for the human diploid cells (KMB17) and has good immunogenicity and heredity stability, and culturing a rabies vaccine virus seed (CTN-DK strain) capable of efficiently reproducing in the human diploid cells (KMB17). By using the virus seed for producing a human diploid cell (KMB17) rabies vaccine, the risk caused by residual heterogonous DNA (deoxyribonucleic acid) in the vaccine which is currently used at home can be effectively avoided, and the safety and practicability of the rabies vaccine in China are further improved, thus the invention has great social and economic benefits.

The invention provides applications of fructus momordicae residue and the root of sprouted beans in production of a mushroom culture material. According to the present invention, 45-55 parts by weight of fructus momordicae fruit residue and 25-35 parts by weight of the root of sprouted beans are adopted as the carbon source of a culture material, and 16-20 parts by weight of bran, 1-2 parts by weight of sucrose, 0.1-0.5 part by weight of urea and 0.3-0.6 part by weight of calcium superphosphate are supplemented to prepare the culture material; and with the culture material, the problems of less source and high price of the carbon source main material for the mushroom culture are well solved.