The invention discloses a three-dimensional pipeline intelligent control soilless culture system, which comprises a liquid storage pipe (1) sealed by a sealing cover, a liquid return pipe (5), a main pipeline bracket (13), a main liquid inlet pipe (17), a culture pipe (6) with planting holes, liquid level adjusting water outlet seal heads (22), an upper liquid outlet pipe (16), a lower liquid inlet pipe (15), a circulating water pump (10), an intelligent automatic controller (18), a standby liquid charging cup (26), a first concentrate preparation cup (3), a second concentrate preparation cup (3), a water inlet joint electromagnetic valve (7), a water level detector (19), a main pipeline bracket connecting port (25), a liquid supply pipe (12) and the like, wherein the liquid level adjusting water outlet seal heads (22) are arranged at the two ends of the culture pipe (6); and the standby liquid charging cup (26) is arranged on the top of the liquid storage pipe (1). The three-dimensional pipeline intelligent control soilless culture system has the advantages of rational structure, flexibility and convenience in assembly and disassembly, high automation degree, water saving, power saving, low use cost, full nutrient absorption by vegetables, high growth speed, small floor area and large culture area, and is suitable for being used in families, hotels, exhibition halls and the like.