Double-layer assembly type cytomechanics loading carrier device

Abstract

The invention relates to a carrier device for conducting tensile stress loading on cells, and the device is composed of an upper layer clamping clamp, an intermediate layer clamping clamp, a lower layer clamping clamp, multiple screws, an upper layer silicone rubber membrane and a lower layer silicone rubber membrane. The area of a cell culture basement membrane is increased through the clamping clamps and the screws, meanwhile, double layer design is adopted, and the effective culture area of each stressed cell is greatly increased. By means of the double-layer assembly type cytomechanics loading carrier device, effective mechanical loading can be conducted on the four layers of cells (the front side and the reverse side of the basement membrane) at the same time, and the requirement of the high throughput test for the number of cells is satisfied. The clamps, the screws and the basement membrane are all made of transparent materials, and the biocompatibility is excellent. Real-time microscopical cell morphology observation can be achieved. The whole device is simple and fast in assembling process and cell inoculation process, after being assembled, the device is fitted to all types of existing mechanical loading equipment with axial tension as the design principle as a whole, and loading and unloading are both convenient and efficient. The whole carrier device can be sterilized once again and used repeatedly, the experimental process is simplified, and experiment efficiency is greatly improved.

Description

technical field [0001] The present invention relates to a carrier device for carrying out stretch stress loading on cells, and in particular relates to the need to apply mechanical stimulation to a large number of cells, so as to solve the problem that the effective mechanical loading area of ​​the carrier is small and the number of cells harvested is small in the related cell biomechanical research. The problem of not being able to meet the requirements of high-throughput detection. Background technique [0002] With the rapid development of cell biology, biomechanics and biomaterials, by simulating the biomechanical environment in vivo, it is possible to observe mechanical stimulation on cell proliferation, differentiation and cell physiological functions, and the influence of mechanical microenvironment on cells is becoming more and more serious. More and more attention has been paid to it, and a variety of mechanical loading methods have appeared, such as four-point be...

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Problems solved by technology

Like the four-point bending principle, this design also cannot guarantee the uniformity and homogeneity of mechanical loading

Patent CN1932511A designed a uniaxial sinusoidal tensile mechanical loading device, which can achieve mechanical stimulation of cells by axially stretching the elastic membrane. Although it can meet the loading needs of large strains, it can only be used for one cell at a time. The culture unit is loaded, which affects its scope of application and research efficiency

[0003] The above-mentioned existing patents also have the following shortcomings and deficiencies: 1. The area of ​​the loaded culture plate or the stretched basement membrane is small, which seriously affects the harvested number of target cells and cannot achieve high-throughput detection, such as real-time quantitative PCR and gene detection. chip analysis, etc.

2. The cell loading unit cannot be independent, which is not conducive to loading and unloading and real-time observation of cells under a microscope

3. The process of installing the basement membrane or cell carrier device to the mechanical loading equipment is also relatively complicated, and there are also inconvenient sterilization of the basement membrane and cell inoculation

The above shortcomings seriously limit the use and promotion of elastic basement membrane in the field of cell biomechanics research

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