18 results about "Basement membrane" patented technology

Apparatuses, systems and methods are provided for treating pulmonary tissues via delivery of energy, generally characterized by high voltage pulses, to target tissue using a pulmonary tissue modification system (e.g., an energy delivery catheter system). Example pulmonary tissues include, without limitation, the epithelium (the goblet cells, ciliated pseudostratified columnar epithelial cells, and basal cells), lamina propria, submucosa, submucosal glands, basement membrane, smooth muscle, cartilage, nerves, pathogens resident near or within the tissue, or a combination of any of these. The system may be used to treat a variety of pulmonary diseases or disorders such as or associated with COPD (e.g., chronic bronchitis, emphysema), asthma, interstitial pulmonary fibrosis, cystic fibrosis, bronchiectasis, primary ciliary dyskinesia (PCD), acute bronchitis and / or other pulmonary diseases or disorders.

The invention relates to the field of biological materials, in particular to a preparation method of a bioprosthetic valve, the bioprosthetic valve is obtained by wrapping a cross-linked extracellular matrix material with a non-cross-linked biological basement membrane to form a sandwich structure, and the preparation method specifically comprises the following steps: respectively preparing the non-cross-linked biological basement membrane and the cross-linked extracellular matrix material; and the collagen derivative mixed solution with the area concentration of 8-50 [mu] L / cm < 2 > and the activator mixed solution with the area concentration of 1-10 [mu] L / cm < 2 > are combined and laminated. The advantages of good regeneration and repair performance and carrying of rich bioactive factors of the basement membrane are utilized, growth and adhesion of endothelial cells on the surface of the material are promoted, an endothelial structure similar to natural blood vessels is formed, the composite bioprosthetic valve has good anti-calcification performance, adhesion of platelets and blood cells is reduced, and the bioprosthetic valve has good anti-calcification performance. The recovery and reconstruction of the valve function are accelerated; moreover, erythrocyte rupture and dissolution are not easily caused, the damage degree to erythrocytes is low, and the blood coagulation risk is low.

The present application relates to the field of biomaterials, and particularly relates to a biomimetic biological valve. The biomimetic biological valve is obtained by wrapping a non-crosslinked biological basement membrane around a crosslinked extracellular matrix material to form a sandwich structure. The basement membrane cooperates with the crosslinked extracellular matrix material, fully plays the excellent regenerative repair performance and the characteristics of being rich in bioactive factors of the basement membrane, effectively promotes the growth and adhesion of endothelial cells on the surface of the material, and constructs a tissue structure similar to the natural valve leaflet. The biomimetic biological valve exhibits excellent anti-calcification performance and excellent dynamic mechanical stability and compliance, significantly inhibits the abnormal adhesion of platelets and blood cells, greatly improves the recovery and reconstruction efficiency of the valve function, and can maintain the structural integrity in repeated contraction-expansion cycles, providing an ideal solution for long-term stable application in a physiological environment.

This invention discloses an oral ablation detection method and system, belonging to the field of oral detection technology, including: S1, oral tissue sampling; S2, motion compensation; S3, image reconstruction; S4, lesion analysis; S5, ablation planning; S6, ablation execution. This invention utilizes phase-amplitude joint displacement estimation, extracting depth-direction micro-displacement using phase difference and lateral displacement using amplitude centroid shift. This effectively compensates for non-rigid tissue peristalsis caused by swallowing and tongue movements without relying on external markers or high-frequency frame rates, ensuring that the subsequently reconstructed three-dimensional image and ablation target area localization are not distorted due to motion, thus improving the targeting consistency between detection and treatment. Texture analysis at different scales is applied along the oral mucosal epithelium, basement membrane zone, and lamina propria, and depth-adaptive weights are assigned based on the pathological sensitivity differences of each layer, highlighting the probabilistic response of early cancerous areas.

This invention discloses an engineered extracellular vesicle and its application in the preparation of products for treating autoimmune diseases. The engineered extracellular vesicle is either an IDO1-overexpressing engineered extracellular vesicle (MSC-IDO1-EVs) or a CSF1-overexpressing engineered extracellular vesicle (MSC-CSF1-EVs). The engineered extracellular vesicles provided by this invention exhibit significant immunosuppressive effects, reducing the secretion of inflammatory cytokines. In animal models, they effectively reduce proteinuria levels, significantly reduce blood dsDNA / ANA levels, effectively alleviate kidney damage caused by autoimmune diseases such as renal immune complex deposition, diffuse thickening of the glomerular basement membrane, and mesangial structural disorder, and reduce overall inflammation levels in mice. The therapeutic effect is significantly superior to that of unmodified MSC-EVs. The engineered extracellular vesicles provided by this invention significantly improve the therapeutic effect on autoimmune diseases.

This document relates to methods and materials involved in identifying and / or treating mammals having membranous nephropathy (e.g., membranous nephropathy with an elevated level of a neural epidermal growth factor (EGF)-like 1 (NELL-1) polypeptide in the glomerular basement membrane (GBM)). For example, methods and materials for administering one or more immunosuppressive agents (e.g., corticosteroids, cyclosporine, or a B-cell reduction or depletion agent such as Rituximab) to treat a mammal (e.g., a human) having membranous nephropathy are provided.

The application relates to the technical field of traditional Chinese medicines, and discloses application of a traditional Chinese medicine composition in preparation of a medicine for preventing or treating diabetic microangiopathy, wherein the traditional Chinese medicine composition can improve blood perfusion and blood oxygen content of the foot skin of a diseased rat, reduce whole blood viscosity, has a certain protective effect on eyeball retina injury of a diabetic vasculopathy rat, inhibits infiltration of inflammatory cells in kidney tissue of a diabetic microangiopathy rat, improves glomerular basement membrane morphology, improves edema of proximal tubular epithelial cells, has a certain protective effect on foot skin injury of a diabetic microangiopathy rat, and reduces ET-1 level and Cys-C level in serum.

The invention discloses application of hydrogel in preparation of an anti-infection and anti-scar repair external preparation for treating and chemotherapy skin wounds, and belongs to the field of biomedical materials. The hydrogel comprises sodium alginate and a copper ion solution, the mass fraction of the copper ion solution is 0.01%-0.05%, and 0.1%-1% of sodium alginate is added into every 100 mL of the copper ion solution according to the mass fraction; the external preparation realizes skin wound infection resistance and scar inhibition after chemotherapy by regulating and controlling the expression of collagen and basement membrane protein in wound tissues. According to the application, bacterial contamination can be effectively inhibited, up-regulation of collagen III and Laminin beta1 is promoted, abnormal hyperplasia of collagen I and collagen VII is inhibited, a soft and regular reticular collagen structure is formed on a wound surface, and HE dyeing shows that dermal fibers are uniform and ordered in direction, and scar tissues are obviously reduced.

This invention discloses a method for constructing a mouse model of COL4A5-K229X point mutation X-linked Alport syndrome. Cas9 mRNA, gRNA, and a donor vector were designed and prepared targeting the c.685A>T point mutation in exon 12 of the mouse COL4A5 gene. These components were mixed and microinjected into C57BL / 6J mouse zygotes to obtain F0 generation mice. Genotypes were identified by PCR amplification and Sanger sequencing, and positive mice were screened. Positive F0 generation mice were mated with wild-type mice to breed F1 and subsequent generations. The model phenotype was further verified by qPCR, biochemical analysis, light microscopy, transmission electron microscopy, and immunofluorescence. The model constructed by this invention exhibits hematuria, proteinuria, azotemia, podocyte loss, and irregular thickening and stratification of the glomerular basement membrane, consistent with the phenotype of human XLAS patients, providing an animal model tool for elucidating the pathogenesis and developing treatment strategies.

This document relates to methods and materials involved in identifying and / or treating mammals having membranous nephropathy (e.g., membranous nephropathy with an elevated level of a Semaphorin 3B polypeptide in the glomerular basement membrane (GBM)). For example, methods and materials for administering one or more immunosuppressive agents (e.g., corticosteroids, cyclosporine, or a B-cell reduction or depletion agent such as Rituximab) to treat a mammal (e.g., a human) having membranous nephropathy are provided.

The invention belongs to the technical field of biological materials, and particularly relates to a functional sebaceous gland-skin integrated model based on biological 3D printing and a construction method and application of the functional sebaceous gland-skin integrated model. The double-nozzle collaborative printing technology is adopted, a complete sebaceous gland-catheter-epidermis secretion path is successfully constructed, directional transportation of lipid secretion is achieved, and seamless communication between the catheter and the epidermis is achieved. Meanwhile, specific bio-ink is selected in the printing process, the mechanical stability of the model can be remarkably improved, and the collapse rate within 7 days after printing can be controlled to be 5% or below. The model precisely simulates natural anatomical characteristics in structure, the mechanical property is close to that of a natural catheter basement membrane, the problems that a secretion path is blocked, a catheter is prone to collapse and the like generally existing in a traditional model are effectively solved, and more reliable model support is provided for related research and application.

Disclosed herein are methods for utilizing and forming suspension cultures of pluripotent stem cells (PSCs), and differentiated cells, spheroids, and organoids derived from PSCs (e.g., with industrial efficiency and / or scalability), as well as compositions comprising them. These methods can be carried out in suspension culture without the use of a basement membrane matrix during the maintenance and expansion of PSCs, and during the differentiation of PSCs into differentiated cells and organoids, such as endoderm (DE), hindgut spheroids (HGS), and intestinal organoids (IO). In some embodiments, the methods may be xenofree and may be carried out in accordance with Good Manufacturing Practices (GMP). Also disclosed herein are methods for controlling the polarity of epithelial cells in IOs, such that the apical layer is oriented outside the organoid, or alternatively inside the organoid. The use of methods and compositions for transplantation and therapeutic purposes is also disclosed.

Disclosed are compositions and methods of making basement membrane constructs having interior or luminal volumes. The interior or luminal volumes may be in the form of vascular networks for liquid (e.g., blood) or gas perfusion. The interior spaces may also contain cells, such as epithelial cells. Also disclosed are tissues and organs, and methods of making thereof, comprising basement membrane constructs.

The invention relates to the field of biological materials, in particular to a bionic biological valve. The bionic bioprosthetic valve is obtained by wrapping a cross-linked extracellular matrix material with a non-cross-linked biological basement membrane to form a sandwich structure. The basilar membrane and the cross-linked extracellular matrix material are synergistic, the excellent regeneration and repair performance and the characteristic of being rich in bioactive factors of the basilar membrane are fully exerted, growth and adhesion of endothelial cells on the surface of the material are effectively promoted, and a tissue structure imitating a natural valve leaflet is constructed. The biomimetic bioprosthetic valve shows excellent anti-calcification performance and excellent dynamic mechanical stability and compliance, significantly inhibits abnormal adhesion of platelets and blood cells, greatly improves the recovery and reconstruction efficiency of valve functions, can maintain structural integrity in repeated contraction-expansion cycles, and has good application prospects. And an ideal solution is provided for long-term stable application of the compound in a physiological environment.

The invention belongs to the technical field of bioengineering, and particularly relates to recombinant human IV-type collagen as well as a preparation method and application thereof. According to the application, a segment from natural IV type collagen is selected, expression in escherichia coli is improved by optimizing an auxiliary element, and meanwhile, it is verified that the recombinant human IV type collagen can remarkably promote proliferation of human skin fibroblasts, promote senescent human primary dermal fibroblasts to generate energy and recover cell viability, so that the recombinant human IV type collagen can be used for improving the human skin fibroblasts. The method has good application potential in preparation of cosmetics and skin care products in the aspects of substrate membrane stability maintaining, repairing and the like.

The invention belongs to culture of tumor organoids, and particularly relates to a preparation method of tumor organoids, which comprises the following steps: digesting a lung tumor sample to obtain an epithelium starting material, performing three-dimensional culture on a basement membrane matrix to form primary organoids, and performing early cryopreservation in a main library; homologous organs are homogenized and subpackaged into test groups, and after adaptive culture is carried out under a combination of multiple culture media, chemotherapy pressure is applied, and elution recovery is carried out; a target culture medium combination for screening the drug-resistant subgroups is determined according to survival and regrowth and in combination with a normal sample removal result of morphology; according to the method, the proportion of tumor components is increased, the drug-resistant survival subgroups are enriched, and the preparation consistency and repeatability are improved.

The application of F5-peptide and / or P-gp inhibitor in the preparation of drugs crossing blood-testis barrier and oral targeted nanoparticles and drugs, for the first time, F5-peptide is used to destroy the physical barrier of BTB, and Tariquidar is used to inhibit the biochemical barrier of BTB, so that the BTB is instantaneously opened and closed, the basal ES protein and the tight junction protein are no longer closely connected with the BTB near the basement membrane, and the P-gp transporter cannot pump the exogenous drug Adjudin out of the BTB, so that the Adjudin is successfully transported to the spermatogenic epithelium, and the FAF5T-LAN oral targeted nano-preparation carrying active Adjudin molecules is constructed, and is expected to become a high-compliance male contraceptive, and has important social significance and economic value.

The invention relates to the technical field of tissue / organ bionic manufacturing materials, in particular to an in-vitro bionic skin model and a preparation method thereof. The preparation method of the in-vitro bionic skin model comprises the following steps that 1, a microfiber material is deposited on an electrostatic spinning nanofiber membrane through 3D printing, and a bionic basement membrane of a fluctuating structure is formed; 2, sequentially soaking the bionic basement membrane with the periodically fluctuating microstructure in a first solution and a second solution to prepare a polydopamine coating and a biological functional coating, so as to obtain a bionic basement membrane with a double-layer structure; and step 3, inoculating the epidermal cells to the surface of the bionic basement membrane with a double-layer structure, and sequentially putting the bionic basement membrane into an immersion culture medium and a differential culture medium for immersion culture and gas-liquid interface culture to obtain the in-vitro bionic skin model.