495 results about "Basement membrane" patented technology

The application of matrix metalloproteinase (MMP) inhibitors to the skin inhibits the degradation of proteins found in the skin including collagen, elastin, and other basement membrane and extracellular matrix protein. MMP inhibitors may be used in both cosmetic compositions and pharmaceutical compositions for application to skin. MMP inhibitors are formulated with a cosmetically suitable vehicle or pharmaceutically acceptable excipient for application to the skin as creams, lotions, ointments, solutions, face masks, etc. As cosmetics, the inventive MMP inhibitor compositions are applied to the skin to prevent or reduce the appearance of wrinkles, pigmentation changes, loss of elasticity, or other effects associated with aging or sun damage. As pharmaceuticals, the inventive MMP inhibitor compositions may also be applied to the skin to treat or prevent a skin disease (e.g., proliferative disease, inflammatory disease).

Described herein are tissue grafts derived from the placenta. The grafts are composed of at least one layer of amnion tissue where the epithelium layer has been substantially removed in order to expose the basement layer to host cells. By removing the epithelium layer, cells from the host can more readily interact with the cell-adhesion bio-active factors located onto top and within of the basement membrane. Also described herein are methods for making and using the tissue grafts. The laminin structure of amnion tissue is nearly identical to that of native human tissue such as, for example, oral mucosa tissue. This includes high level of laminin-5, a cell adhesion bio-active factor show to bind gingival epithelia-cells, found throughout upper portions of the basement membrane.

The invention provides a self-assembling peptide comprising (a) a first amino acid domain that mediates self-assembly, wherein the domain comprises alternating hydrophobic and hydrophilic amino acids that are complementary and structurally compatible and self-assemble into a macroscopic structure when present in unmodified form; and (b) a second amino acid domain that does not self-assemble in isolated form. In certain embodiments of the invention the second amino acid domain comprises a biologically active peptide motif, e.g., a peptide motif found in a naturally occurring protein, or a target site for an interaction with a biomolecule. In certain embodiments of the invention the naturally occurring protein is a component of the extracellular matrix, e.g., a component of the basement membrane. The invention further provides scaffolds comprising the self-assembling peptides and methods of using the scaffolds including for cell culture, tissue engineering, and tissue repair.

Described herein are tissue grafts derived from the placenta. The grafts are composed of at least one layer of amnion tissue where the epithelium layer has been substantially removed in order to expose the basement layer to host cells. By removing the epithelium layer, cells from the host can more readily interact with the cell-adhesion bio-active factors located onto top and within of the basement membrane. Also described herein are methods for making and using the tissue grafts. The laminin structure of amnion tissue is nearly identical to that of native human tissue such as, for example, oral mucosa tissue. This includes high level of laminin-5, a cell adhesion bio-active factor show to bind gingival epithelia-cells, found throughout upper portions of the basement membrane.

Matrix metalloproteinases (MMPs) are a group of enzymes that have been implicated in the pathological destruction of connective tissue and basement membranes. These zinc containing endopeptidases consist of several subsets of enzymes including collagenases, stromelysins and gelatinases. TNF-alpha converting enzyme (TACE), a pro-inflammatory cytokine, catalyzes the formation of TNF-alpha from membrane bound TNF-alpha precursor protein. It is expected that small molecule inhibitors of MMPs and TACE therefore have the potential for treating a variety of disease states. The present invention provides low molecular weight, non-peptide inhibitors of matrix metalloproteinases (MMPs) and TNF-alpha converting enzyme (TACE) for the treatment of arthritis, tumor metastasis, tissue ulceration, abnormal wound healing, periodontal disease, bone disease, diabetes (insulin resistance) and HIV infection having the formulawherein R2 and R3 form a heterocyclic ring and A is S, S(O), or S(O)2, and R1 and R4 are defined herein.

An object of the present invention is to provide a method for producing an ADM suitable as a scaffold for a cultured skin, that is, a separation and decellularization method that enables various types of extracellular matrixes such as a basement membrane to be preserved, allows the epidermal layer to be peeled off easily, and does not cause any damage to the dermal matrix. The present invention relates to a skin separation and decellularization method comprising a step of freeze thawing harvested skin and then separating the skin into epidermis and dermis by a treatment with hypertonic saline, and a step of washing the separated dermis.

The present invention relates to a dermal substitute comprising the biodegradable polymer such as collagen and the biomaterial such as amnion, the preparation method and the use thereof. Specifically, the present invention provides with an amnion-collagen sponge complex structure prepared by attaching, inserting or incorporating an amnion obtained from placenta to / in collagen. Inventive dermal substitute can be applied to surgery and wound requiring skin graft, for example, severe burns such as second-degree burn, without rejection by immune system. Further, inventive dermal substitute with amnion instead of silicone membrane has several advantages, such as better biocompatibility, anti-inflammatory activity and promoting activity of wound healing and commercial utilization as basement membrane. Also, inventive complex structure can be used as the basic matrix of bio-artificial skin for culturing cells and the biodegradable basic matrix for preparing artificial organs.

The invention discloses a crosslinking hyper branched polymer composite nanofiltration membrane as well as the preparation method thereof. The crosslinking hyper branched polymer composite nanofiltration membrane is prepared by taking an ultrafiltration membrane as a basement membrane and crosslinking hyper branched polymer as a selecting layer through hyper branched polymer and the interfacial polymerization of polybasic acid, polybasic acyl chloride, polybasic anhydride and polybasic amine; and the interfacial polymerization takes the mixed solution of water and ethanol as the water phase and n-hexane, n-heptane or n-octane as the organic phase. As the hyper branched polymer has the spheroidal structure, a plurality of nano-voids exist in the interior of the molecule, so as to enable the selecting layer of the crosslinking hyper branched polymer composite nanofiltration membrane to be looser, and leads the nanofiltration membrane to maintain high flux and retention rate under the lower operating pressure. The nanofiltration membrane can be used in the fields of medicament, foodstuff, environmental protection, etc. The composite nanofiltration membrane is applicable to the separation and the condensation of high valence ions, low valence ions, neutral particles, drugs, food additives, etc.

The invention discloses a lithium-ion battery separator subjected to composite coating treatment and a preparation method thereof. The lithium-ion battery separator comprises a basement membrane, a polymer coating coated at one side of the basement membrane, and an inorganic particle coating coated at the other side of the basement membrane; the polymer coating is composed of an aramid coating coated on the basement membrane and a polyvinylidene fluoride coating coated on the aramid coating, and the thickness of the polymer coating is 0.3 to 5 [mu] m; and the inorganic particle coating is composed of inorganic particles of different particle sizes and a binding agent, and the thickness of the inorganic particle coating is s 0.5 to 5 [mu] m. The lithium-ion battery separator provided by the invention has the characteristics of being excellent in mechanical property and heat resistant property, having good wettability and solution preserving property to electrolyte, and excellent in cycle performance and high in safety performance.

The invention relates to the field of lithium ion battery diaphragm preparation and relates to an inorganic coating high-temperature resisting type lithium ion battery diaphragm with a special structure and the preparation method thereof. The lithium ion battery diaphragm comprises a porous flexible basement membrane and coatings coated on the two sides of the basement membrane, and composites of inorganic particles and organic microsphere are contained in the coatings. The inorganic particles and the organic microspheres are mixed into the solvent according to a certain proportion, homogeneous membrane coating slurry can be obtained, the slurry is coated on the two surfaces of the flexible and organic basement membrane, and then, under a certain temperature, the composite diaphragm with favorable performance is obtained through the pressing of a hot roller. In the inorganic coating lithium ion battery diaphragm provided by the invention, bonding agent is not directly added into the membrane coating slurry, damages to the microstructure of the diaphragm caused by the bonding agent can be avoided, the high-temperature resisting performance and the electrolyte infiltrating performance of the diaphragm are greatly improved, and the composite lithium ion battery diaphragm prepared by the invention has the advantages of superior comprehensive performance, easiness in big-scale and large-dimension production and the like.

The present invention relates to methods and compositions designed for the treatment, management, or prevention of cancer, particularly, metastatic cancer. In one embodiment, the methods of the invention comprise the administration of an effective amount of one or more antibodies that bind to EphA4 and agonize EphA4. In another embodiment, the methods of the invention comprise the administration of an effective amount of one or more antibodies that bind to EphA4 and inhibit cancer cell colony formation in soft agar or tubular network formation in three-dimensional basement membrane or extracellular matrix preparation. In another embodiment, the methods of the invention comprise the administration of an effective amount of one or more antibodies that preferentially binds to an EphA4 epitope that is exposed on cancer cells but not non-cancer cells. In another embodiment, the methods of the invention comprise the administration of an effective amount of one or more antibodies that bind to EphA4 with a very low Koff to reduce EphA4 expression and, thereby, inhibit tumor cell growth and / or metastasis. The invention also provides pharmaceutical compositions comprising one or more EphA4 antibodies of the invention either alone or in combination with one or more other agents useful for cancer therapy.

The invention discloses a preparation method of a hydrophilic separation membrane. The separation membrane comprises a basement membrane support layer and a hydrophilic separation membrane layer. According to the method, by membrane surface chemical reaction, a hydrophilic substance is introduced into hydroxylated-treated basement membrane support layer surface to form the hydrophilic separation membrane layer. The separation membrane has a good hydrophilic property and high pure water flux. The preparation method of the hydrophilic separation membrane is a simple, effective and practical membrane preparation method. The separation membrane can be used in water treatment, food, pharmaceuticals, petrochemical industry and other fields.

The invention relates to a semi-active control magnetorheological hydraulic mount for an automotive powertrain, which belongs to a semi-active control element used for vibration control of an engine and is an engine mount filled with magnetorheological fluid, wherein the viscosity of the fluid changes along with the change of the strength of a magnetic field, and the semi-active control magnetorheological hydraulic mount mainly comprises a metal framework, a rubber main spring, a coil holder, an electromagnetic coil, a magnetic conductive channel body, an inertia channel assembly, a rubber basement membrane, a bottom framework and the like, and can control the strength of applied current according to the working condition of the engine by utilizing the viscosity of the magnetorheological fluid and the following performance of the strength of the magnetic field, further change the viscosity of the fluid, lead the vibration of the engine to be well attenuated in a wider frequency band and further actively isolate the transfer of vibration noise of the engine into an automobile in the wider frequency band. A vibration damper for the magnetorheological hydraulic mount for the engine can effectively isolate the vibration of the engine, reduce the noise in the automobile and improve the NVH properties of the whole automobile, thereby having great market potential and broad prospects.

A method for surgical repair of damaged or diseased head and neck tissues is described. In one aspect of the invention tissue graft constructs comprising vertebrate submucosa or vertebrate basement membrane materials are used to repair and promote growth of endogenous vocal cord tissue.

The present invention provides a dried amnion which is dried with maintaining tissues of a raw amnion and can be easily stored for a prolonged period of time. An amnion which is dried with maintaining cells and tissues of a raw amnion can be produced by repeating a pressure-reducing operation and a pressure-recovery operation several times, the pressure-reducing operation comprising continuously heating a raw amnion placed in a treatment vessel by a far-infrared heater provided in the treatment vessel and reducing the pressure of the inside of the treatment vessel, and the pressure-recovery operation comprising recovering the reduced pressure of the inside of the treatment vessel with heating the amnion by microwaves irradiated from a microwave heating apparatus provided outside of the treatment vessel, and the amnion is characterized by retaining basement membranes and connective tissues which are constituents of the raw amnion.

The invention discloses a preparation method of a nano silver / poly dopamine composite membrane. The preparation method comprises following steps: phase inversion is adopted so as to prepare a polyether sulfone (PES) ultrafiltration basement membrane; the PES ultrafiltration basement membrane is immersed in a dopamine solution, and an ultrathin active layer is formed via deposition of poly dopamine onto the surface of the PES ultrafiltration basement membrane; in-suit introduction of nano silver onto the membrane surface is realized via silver nitrate postprocessing, and the nano silver / poly dopamine composite membrane is prepared. Advantages of the preparation method are that: compared with the preparation method of conventional nano silver composite membranes, poly dopamine is taken as a multifunctional unit, and construction of a separation layer, mineralization, and immobilization of nano silver are realized. The preparation method is green, simple, convenient, and controllable; the separation layer is obtained via water phase construction, and no organic solvent is needed; in-suit synthesis of nano silver is realized, and no chemical reductant or photo-initiation is needed. The nano silver / poly dopamine composite membrane possesses relatively high water flux, and excellent dye separation performance and antibacterial properties.

The invention discloses a PDMS / PVDF composite membrane for separating organic vapor which pertains to the technical field of vapor permeation membrane separation and a preparation method thereof. The steps to prepare the composite membrane are that: polyvinylidene fluoride is firstly dissolved in a solvent to prepare a basement membrane and the basement membrane is dried, treated by a surfactant and reserved. Silicone rubber is dissolved in normal heptane to be prepared into a uniform membrane casting solution; then the filtered and defoamed membrane casting solution is coated on the treated basement membrane and PDMS / PVDF composite membrane is obtained after being cured at different temperatures. The PDMS separation layer prepared by the invention is uniformly distributed, compact and sound, and is closely combined with the PVDF. The obtained PDMS / PVDF composite membrane by preparation is applied to separating dimethylmethane / N2 system, the total flux reaches 615*10<6>cm<3>(STP) / (cm<2>s cmHg) and the separation factor is larger than 20.

Therapeutic compounds and methods for inhibiting amyloid deposition in a subject, whatever its clinical setting, are described. Amyloid deposition is inhibited by the administration to a subject of an effective amount of a therapeutic compound comprising an anionic group and a carrier molecule, or a pharmaceutically acceptable salt thereof, such that an interaction between an amnyloidogenic protein and a basement membrane constituent is inhibited. Preferred anionic groups are sulfonates and sulfates. Preferred carrier molecules include carbohydrates, polymers, peptides, peptide derivatives, aliphatic groups, alicyclic groups, heterocyclic groups, aromatic groups and combinations thereof

Therapeutic compounds and methods for inhibiting amyloid deposition in a subject, whatever its clinical setting, are described. Amyloid deposition is inhibited by the administration to a subject of an effective amount of a therapeutic compound comprising an anionic group and a carrier molecule, or a pharmaceutically acceptable salt thereof, such that an interaction between an amyloidogenic protein and a basement membrane constituent is inhibited. Preferred anionic groups are sulfonates and sulfates. Preferred carrier molecules include carbohydrates, polymers, peptides, peptide derivatives, aliphatic groups, alicyclic groups, heterocyclic groups, aromatic groups and combinations thereof.

Heparinase enzymes can be used as a medical treatment to reduce localized inflammatory responses. Treatment of activated endothelium with heparinase inhibits leukocyte rolling, adhesion and extravasation. Most of the heparin and heparan sulfate on endothelial cell surfaces and in basement membranes is degraded by exposure to heparinase. In addition, immobilized chemokines, which are attached to heparin / heparan sulfate on activated endothelium are solubilized by heparinase digestion. Heparinase can be infused into the vascular system to inhibit accumulation of leukocytes in inflamed tissue and decrease damage resulting from localized inflammations. Targeting of heparinase to activated endothelium can be accomplished through localized administration and / or use of genetically engineered heparinase containing endothelium ligand-binding domains.

A preparation method of a high-performance PVDF composite ultrafiltration membrane with the surface loaded with a TiO2 thin membrane is provided. The invention is used for solving the problems of complex process, high membrane preparing cost, and difficult industrialized production existing in a conventional method for improving membrane hydrophilicity so as to improve membrane flux, anti-fouling performance and the like. The preparation method comprises the steps: 1, preparing a PVDF polymer membrane casting solution, and preparing an ultrafiltration basement membrane by an immersion-precipitation method; 2, preparing an auxiliary agent solution; 3, soaking the basement membrane in the auxiliary agent solution, taking out and swashing with water, and soaking in deionized water; 4, preparing a titania precursor solution; 5, immersing the basement membrane in the titania precursor solution, hydrolyzing a precursor, taking out and swashing with water, and thereby obtaining the high-performance ultrafiltration membrane with the surface firmly loaded with the TiO2 thin membrane. The method of the invention has the advantages of simple operation and low cost; and the obtained ultrafiltration membrane has large permeation flux, high rejection rate, strong hydrophilicity and outstanding anti-fouling performance. The preparation method is used for the membrane processing field.

The invention provides a reflection holographic thin membrane and a preparation method thereof. The reflection holography membrane comprises a basement membrane, a buffer layer which is coated at one side of the basement membrane, a photopolymer coating which is coated at the other side of the buffer layer and formed by the holographic polymer paints, and a protective membrane which is covered on the surface of the photopolymer; wherein, hologram images or the interference fringes of double discoloration pictures are recorded on the photopolymer coating; the photopolymer coating comprises film-forming agents, a nano-composite pre-polymer, a monomer, photo initiators, chain transfer agents and photo sensitizers; the nano-composite pre-polymer is a product which is prepared by alkenyl unsaturated monomer with an unsaturated group at the terminal position and nano-particles. The invention has high sensitivity, high reflection efficiency, high refractivity modulation value, long storage life and little influence of the environment on the hologram images; the recorded images are treated by photo-curing and heat-enhancement and can reach reflected images and double discoloration images with the reflection efficiency of more than 99.9 precent, so mass production is available.

The present invention relates generally to a tissue preparation including tissue cells and extracts thereof useful for promoting or facilitating the growth, development and differentiation of cells and tissues. More particularly, the present invention provides muscle-derived material comprising intact or extracted extracellular matrix and / or cells as well as cytokines, growth factors and other components. The muscle preparations of the present invention resemble basement membrane and are derived from cellular-based material. The muscle preparation may be used in vitro or in vivo as inter alia, a cellular scaffold in various tissue engineering applications and in other cell culture systems for nurturing and enriching a range of cell types including, but not limited to, precursor and stem cells such as pre-adipogenic cells. The muscle preparation is also useful as a base for creams, such as in the cosmetic and topical therapeutic industries and as a matrix or additive in the food industry.

The invention relates to an optical polyester film with multilayer structure and preparation method thereof. The film comprises a basement membrane, at least one side of the basement membrane is coated with a ground coat, the basement membrane comprises a sandwich layer and at least one surface layer, wherein the sandwich layer contains nano additive, and the surface layer contains nano additive and micron additive; the ground coat is an organic polymer adhesive layer, the surface hardness of the ground coat is more than or equal to 1H. The film obtained by the invention has low haze value, excellent optical property, favourable surface adhesiveness, heat resisting property, surface smooth property, anti-sticking property and processability, and can be applicable to the display device fields of LCD, CRT, PDP, EL and the like.

The invention relates to pearlescentlaser paper and a preparation process thereof. The pearlescent laser paper comprises a finishing coat, a laser layer, a pearlescent transfer layer, an aluminizing or dielectric coating layer, a transfer glue layer, base paper and a back coating. The preparation process comprises the following steps of firstly, coating pearlescent pigment on a Bopp basement membrane with the laser layer to form the pearlescent transfer layer; secondly, performing surface tension enhancing treatment on the pearlescent transfer layer; thirdly, performing aluminizing or dielectric coating on the pearlescent transfer layer to form the aluminizing or dielectric coating layer; fourthly, coating transfer glue on the aluminizing or dielectric coating layer to form the transfer glue layer; fifthly, performing back coating on the back side of the base paper to form the back coating layer, and compounding the front side of the base paper with the transfer glue layer; sixthly, stripping off the Bopp basement membrane; seventhly, performing finishing coating on the laser layer to form the finishing coating layer. The paper disclosed by the invention has a stronger laser diffraction effect and a colorful twinkling effect of the pearlescent pigment, so that the application grade is improved; the Bopp basement membrane in the preparation process can be repeatedly used, so that the production cost of products is greatly reduced.

The invention relates to a white covering film and a manufacturing method thereof. The white covering film comprises a release basement membrane, a white coating coated on the release basement membrane and an adhesive layer coated on the white coating. The white coating is a composition using flexible saturated polyester as a matrix and comprises the following components in part by weight: 50 to 70 parts of flexible saturated polyester, 30 to 50 parts of hard saturated polyester, 1 to 20 parts of blocked isocyanate curing agent, 15 to 150 parts of pigment, 15 to 120 parts of inorganic filler, 0.01 to 0.30 parts of fluorescent brightening agent, 0.5 to 2.5 parts of antifoaming agent and a right amount of solvent. The white covering film can be used for a flexible printed circuit and has high yellowing resistance, high covering power, excellent folding resistance and high glossiness. When the white covering film is compressed with a substrate, the excellent adhesion between the white covering film and the substrate is guaranteed.

The invention discloses a composite membrane and a preparation method, aims at improving the bonding firmness between a functional layer and a basement membrane and the pressure resistance of the composite membrane and belongs to the field of the preparation of blended high polymer materials and polymeric membranes. According to the method, casting membrane solution containing amino and carboxyl is prepared through an in-situ hybrid polymerization method; the basement membrane is prepared by adopting an immersion phase inversion method to enable the surface of the basement membrane to contain carboxyl and amino radicals, and the basement membrane is bonded with the functional layer through chemical bonds and hydrogen bond, so that the bonding firmness between the functional layer and the basement membrane is improved, and the pressure resistance of the composite membrane is increased.

A tissue graft composition comprising liver basement membrane and a method of preparation of this tissue graft composition are described. The graft composition can be implanted to replace or induce the repair of damaged or diseased tissues.

The invention discloses a making method of hydrophilic nanometer filter membrane, which is characterized by the following: the basement membrane is an optically active polymer ultrafilter membrane, which introduces at least two strong polarity hydrophilic monomers in the photosensitizer or non-photosensitizer condition through irradiation copolymerization grafting method; the nanometer filter membrane displays good hydrophilicity, which possesses high interception rate and effusion flux for II-valence ion.

The invention discloses a composite reverse osmosis membrane with a high salt removing rate and controllable flux and a method for preparing the composite reverse osmosis membrane.The method includes coating water-phase solution layers on a basement membrane with porous polysulfone support layers; coating oil-phase solution layers on the basement membrane; carrying out interfacial polymerization reaction on two phases to obtain polyamide ultrathin functional separation layers; cleaning and drying the basement membrane in follow-up procedures to obtain the composite reverse osmosis membrane.The composite reverse osmosis membrane and the method have the advantages that the composite reverse osmosis membrane produced by the aid of the method is detected by the aid of 1500ppm NaCl solution under the conditions of the pH (potential of hydrogen) of 7.5-8, the temperature of 25 DEG C and the test pressures of 150psi, the salt removing rate is 99.30%-99.75%, and the water flux is 33gfd-50gfd.