49 results about "Type IV collagen" patented technology

The invention provides a micromolecular recombinant XVII type collagen as well as a preparation method and application thereof, and relates to the technical field of protein engineering. The novel recombinant human XVII type collagen is obtained by optimizing and screening a plurality of spiral region sequences such as a collagen structural domain, a C-terminal region and a middle region of the human XVII type collagen. The amino acid composition of the recombinant XVII type collagen is consistent with that of a natural collagen alpha1 chain, immunological rejection is not generated when the recombinant XVII type collagen is applied to a human body, the recombinant XVII type collagen can be widely applied to surgical medical treatment and medical beauty industries, the molecular weight of the collagen is small, efficient secretion soluble expression in eukaryotic host cells such as pichia pastoris can be achieved, and the recombinant XVII type collagen has a good application prospect. And industrial large-scale production is carried out. According to the present invention, the verification results show that the micromolecular recombinant XVII type collagen further has basement membrane anti-aging activity, wherein the basement membrane anti-aging activity specifically comprises the improvement of the expression levels of the IV type collagen, the VII type collagen and the XVII type collagen;

The invention provides recombinant IV type collagen and application thereof, and relates to the technical field of protein engineering. In the invention, an amino acid residue repetitive unit with good biological activity and stability in the human-derived IV-type collagen is found, and the amino acid residue repetitive unit is used as a core for repeating and constructing the recombinant IV-type collagen, so that the novel human-derived recombinant IV-type collagen with biological activity is obtained. The human-derived recombinant IV-type collagen provided by the invention is a small-molecular-weight protein, is easy to absorb by a human body, is good in safety, can remarkably promote secretion of collagen, also has the effects of promoting cell adhesion and cell migration, and has a good application prospect in the aspects of basement membrane repair and remodeling. A new functional component is provided for products for repairing and tightening the skin, resisting aging, resisting wrinkles, preserving moisture and the like.

The invention discloses an IV-type collagen detection kit and a preparation method thereof, and relates to the technical field of in vitro diagnos.The kit comprises a reagent R1 and a reagent R2, and the volume ratio of the reagent R1 to the reagent R2 is 3: 1; through an elaborately designed reagent formula, especially the accurate proportion of all components in the reagent R1, such as ammonium chloride, sodium azide and Brij-35, the detection sensitivity and accuracy are improved, the stability and repeatability of the kit are also ensured, and particularly, the natural plant extract astragalus polysaccharide and the protease K inhibitor are introduced into the reagent R1 as key components, so that the detection sensitivity and accuracy are improved, and the detection sensitivity and repeatability are improved. According to the present invention, the unique preparation method and the activity promotion effect on the IV-type collagen hydrolase significantly enhance the biological activity of the kit, such that the detection result is reliable, and the two latex particles coated with the paired mouse monoclonal antibodies adopted in the reagent R2 have high specificity and affinity, such that the kit can be used for the detection of the IV-type collagen hydrolase. And the detection accuracy and sensitivity are further improved.

Provided herein is a method of biosynthesizing human body structural material type IV collagen. Provided herein is a recombinant collagen including the sequence represented by SEQ ID NO: 1, etc. The recombinant collagen of the present application exhibits excellent cell-adhesion activity. The method of the present application utilizes genetic engineering techniques to produce the recombinant collagen, thereby overcoming the drawbacks of the prior art.

The invention discloses a recombinant IV-type collagen as well as a preparation method and application thereof. The recombinant IV-type collagen is prepared by repeating a sequence as shown in SEQ ID NO.1 for n times (1lt; the recombinant IV-type collagen can effectively promote skin basilar membrane cells to express IV, VII and XVII-type collagen, can repair and reinforce skin barriers, so that the compactness and elasticity of skin are improved, basilar membrane repair is facilitated, the recombinant IV-type collagen can be effectively used for preparing anti-aging products, and the anti-aging effect is good. Wide application prospects are realized in the fields of medical instruments, beauty and skin care and the like. The invention also discloses a method for expressing the recombinant IV type collagen by recombinant escherichia coli, and the method provided by the invention has the advantages of high protein yield, high purity and high yield, and lays a foundation for industrial large-scale production.

The invention belongs to the technical field of skin care products, and relates to full-layer anti-aging recombinant collagen filling powder as well as a preparation method and application thereof. The recombinant collagen filling powder is prepared from recombinant I type collagen, recombinant III type collagen, recombinant IV type collagen, recombinant VI type collagen, recombinant XVII type collagen, superoxide dismutase, a bifidus yeast lysate, trehalose, silica, silica dimethyl silylate and a polydimethylsiloxane / vinyl polydimethylsiloxane cross-linked polymer, and the recombinant collagen filling powder is prepared from the following raw materials: recombinant I type collagen, recombinant III type collagen, recombinant IV type collagen, recombinant VI type collagen, recombinant XVII type collagen, superoxide dismutase, bifidus yeast lysate, trehalose, silica, silica dimethyl silylate and a polydimethylsiloxane / vinyl polydimethylsiloxane cross-linked polymer. The skin care product is prepared from glycerin, xanthan gum, sodium hyaluronate, dimeticone, phenoxyethanol and ethylhexylglycerin. The recombinant collagen filling powder provided by the invention is convenient to use, can obviously improve the skin moisturizing capacity, improve the compactness and repair wrinkles, has a good anti-aging effect and a good repair effect, and has important practical significance and market value.

The application discloses a Mycoplasma bovum adhesion protein FtsZ and application thereof. The nucleic acid sequence of the Mycoplasma bovum adhesion protein FtsZ is shown as SEQ ID NO. 1. The application also discloses a recombinant plasmid pET-30a-ftsZ and an E. coli containing the recombinant plasmid pET-30a-ftsZ. ftsZ The recombinant protein rFtsZ has the advantages of being capable of specifically combining with EBL cell membrane protein, being capable of combining with extracellular matrix components (fibronectin, fibronectin, laminin and type IV collagen), having the direct adhesion host cell effect, having good antigenicity, being capable of producing high-level antibodies, being capable of providing good immune protection effect, and providing a new target and thought for elucidating the pathogenic mechanism of the Mycoplasma bovum and developing a new vaccine and medicine.

This invention provides the application of type IV collagen and its encoding gene in the prognostic assessment and treatment of nasopharyngeal carcinoma (NPC). Research has shown that high expression of type IV collagen and its encoding gene COL4A1 is significantly associated with lower T-cell infiltration in patients with recurrent NPC; compared to NPC patients with low expression of type IV collagen, those with high expression have worse LRRFS and OS; and the expression level of the type IV collagen encoding gene COL4A1 is significantly associated with worse PFS in NPC patients. Therefore, type IV collagen and its encoding gene COL4A1 can serve as specific prognostic biomarkers for assessing the prognosis of NPC patients. Furthermore, inhibiting the expression of type IV collagen can effectively inhibit the growth of NPC; therefore, agents that inhibit type IV collagen expression can be used as drugs to inhibit immune escape in NPC and for the treatment of NPC.

The present invention discloses a type IV collagen detection kit and a preparation method thereof, relating to the technical field of in vitro diagnosis. The kit comprises a reagent R1 and a reagent R2, wherein the volume ratio of the reagent R1 to the reagent R2 is 3:1. The present invention improves the sensitivity and accuracy of detection while ensuring the stability and repeatability of the kit through a carefully designed reagent formula, in particular the precise proportion of the components in the reagent R1, such as ammonium chloride, sodium azide, and Brij‑35. In particular, the reagent R1 introduces a natural plant extract, astragalus polysaccharide, and a proteinase K inhibitor as key components. The unique preparation method and the activity-promoting effect on type IV collagen hydrolase significantly enhance the biological activity of the kit, making the detection results more reliable. In addition, the latex particles coated with two paired mouse monoclonal antibodies used in the reagent R2 have high specificity and affinity, further improving the accuracy and sensitivity of detection.

The invention provides a protein. The protein comprises an amino acid sequence as shown in SEQ ID NO: 3, 4 or 6. In some embodiments, the protein is a FHTFF 1.5 forbidden factor. The protein disclosed by the invention can inhibit angiogenesis by inhibiting proliferation and migration of vascular endothelial cells, and the recombinant protein can also resist telangiectasis below the skin and can also resist blood supply of tumor cells. The invention also provides coding nucleic acid of the protein, a carrier, a cell containing the protein and application of the protein as an innovative biological material in the large health fields of medical instruments, biological medicines, facial beautification, aging resistance and the like.

The present invention provides a recombinant type IV collagen composition for repairing basement membrane and soothing and reducing redness. The inventors found that the recombinant type IV collagen described in the specification of this patent inhibits the phosphorylation of VE-cadherin protein by mediating the PKCα / PYK2 / Src signaling pathway, protects the tight junctions between endothelial cells, prevents the leakage of blood components, and reduces vascular permeability; it can also improve the dissociation of the VE-cadherin complex caused by oxidative stress and maintain the homeostasis of the endothelial barrier; therefore, it has good effects of repairing the basement membrane barrier and soothing and reducing redness. The inventors also first discovered that the repair peptide (GAAGLPGPK) has the effects of repairing the basement membrane barrier, soothing and reducing redness, and the combination of a certain proportion of the recombinant type IV collagen and the repair peptide shows synergistic effects in repairing the basement barrier and soothing and reducing redness.

Immunoassay methods for detecting HNE-generated fragments of the α3 or α4 chain of type IV collagen in a patient sample and their use for detecting and / or monitoring inflammatory bowel disease (IBD) or a particular level of severity of the disease in a patient are disclosed. Also disclosed are monoclonal antibodies and assay kits for use in the immunoassay methods.

The application relates to a preparation method and application of royal jelly acid double-wrapped bodies, and belongs to the technical field of cosmetics. The royal jelly acid double-wrapped body prepared by the application is obtained by co-wrapping royal jelly acid by phospholipid lipids and hydroxypropyl-beta-cyclodextrin, and the double-wrapping system of the hydroxypropyl-beta-cyclodextrin and the liposome improves the loading capacity of the royal jelly acid, has good stability, can significantly improve the solubility of the royal jelly acid, and is convenient for application in cosmetics; compared with pure royal jelly acid, the royal jelly acid double-wrapped body prepared by the application has more excellent skin permeability and more excellent effect of improving the content of type IV collagen of skin, and can provide mechanical support for skin and maintain the young state of skin.

Disclosed herein are medical products, including an implantable device coated with an extracellular matrix comprising Type IV collagen and laminin, wherein the implantable device comprises at least one member selected from the group consisting of sensors, meshes and cannulas, and the extracellular matrix contains no more than 0.024 mg / ml total concentration of glucose, amino acids and salts having a molecular weight of 2000 daltons or less. Corresponding systems and method also are disclosed.

The invention relates to the technical field of cosmetics, in particular to russula vinosa extract and a preparation method and application thereof.The preparation method comprises the steps that dried russula vinosa is smashed, production water is added, heating and stirring are conducted, filtering is conducted, filtrate is reserved, production water is supplemented into filter residues, heating and stirring are continued, filtering is conducted, filtrate is reserved, production water is supplemented into the filter residues again, and the russula vinosa extract is obtained; continuously heating, stirring and extracting, filtering, and retaining filtrate; combining the three filtrates, and carrying out solid-liquid separation to obtain a clear and transparent russula vinosa extracting solution; and concentrating and drying the russula vinosa extracting solution to obtain the russula vinosa extract. The method is simple in process and high in extraction efficiency, meanwhile, the content of type I collagen, type IV collagen and elastin can be remarkably increased on the cellular level by the russula vinosa extract with the concentration of 2 mg / mL, the density of collagenous fibers and elastic fibers can be remarkably increased on human skin explant tissue, and the russula vinosa extract has good anti-wrinkle and firming effects and is suitable for being used as a skin care product. A new raw material choice is provided for preparing the anti-wrinkle and firming cosmetics.

The application relates to the cosmetic technical field, in particular to a high-efficiency anti-aging composition and a skin care product containing the same. The high-efficiency anti-aging composition is prepared from the following raw materials in parts by mass: 12-15 parts of oil-based concentrated active ingredients, 25-35 parts of base collagen liquid, 15-18 parts of ikar, 12-16 parts of TWEEN 20, and 22-25 parts of isosorbide dimethyl ether. The oil-based concentrated active ingredients include safflower seed oil, acmella oleracea flower / leaf / stem extract, Astragalus membranaceus (Fisch.) Bunge root extract, ubiquinone and vitamin E. The base collagen liquid includes type XVII collagen, type VII collagen and type IV collagen. The composition can effectively reduce skin wrinkles, improve skin tightness and reduce skin sagging in a long term, and prevent skin aging, and realizes long-term anti-aging from epidermis to dermis.

This invention belongs to the field of renal pathology testing technology and discloses a method for detecting type IV collagen expression in renal biopsy tissue, comprising the following steps: dewaxing and hydrating paraffin sections of renal biopsy tissue to obtain processed tissue sections; adding the processed tissue sections to a repair solution, heating to boiling, and then cooling to room temperature to obtain repaired tissue sections; adding protease to the repaired tissue sections for enzymatic digestion to obtain enzymatically digested tissue sections; adding a primary antibody to the enzymatically digested tissue sections for preliminary incubation, followed by adding a fluorescein-labeled secondary antibody against the primary antibody for further incubation. This method for detecting type IV collagen expression in renal biopsy tissue is applicable to cases where renal biopsy tissue lacks glomeruli or has too few glomeruli to detect type IV collagen expression. It has high specificity and can provide a new detection method for Alport syndrome in cases where renal biopsy tissue lacks glomeruli.

The present invention provides a hair follicle primordium that can easily and quickly form a hair shaft-like structure in vitro. The method for producing the hair follicle primordium comprises the following steps: seeding epithelial cells and mesenchymal cells; maintaining the epithelial cells and mesenchymal cells in a culture medium containing (a) laminin and entactin, and / or (b) type IV collagen; and co-culturing the epithelial cells and mesenchymal cells in the culture medium.

The present invention provides a recombinant type IV collagen protein and its preparation method and application, which relate to the field of biochemical engineering technology. The preparation method of recombinant type IV collagen protein comprises: obtaining codons through the amino acids of recombinant type IV collagen protein, and then obtaining the target gene, synthesizing it into an expression vector, and obtaining a plasmid; transferring the plasmid into an expression host, screening positive transformants, and obtaining an expression strain; culturing the expression strain, expressing the target protein, and obtaining crude recombinant type IV collagen protein. High-purity recombinant type IV collagen protein is prepared by genetic engineering technology. Recombinant type IV collagen protein can significantly downregulate inflammatory factors and reduce inflammation; it can also reduce heat damage by upregulating heat shock proteins; after heat damage, recombinant type IV collagen protein can significantly downregulate metallomatrix proteases in heat-damaged cells, and can also significantly promote the expression of type I and type III collagen genes, improve skin elasticity and moisture content, and delay skin aging.

The present invention provides an in situ gene transfection system based on a hydrogenated TiO2 nanotube composite coating, as well as its construction method and application. The in situ gene transfection system uses hydrogenated TiO2 nanotubes as a substrate and utilizes a layer-by-layer self-assembly technique to prepare a dopamine / graphene oxide / type IV collagen composite coating. This system can enhance the surface bioactivity of biomedical metal materials while also serving as an in situ gene transfection system for substrate-mediated local viral delivery, improving gene transfection efficiency and treatment safety, and enabling precise regulation of the treatment site. The in situ gene transfection system provides a new strategy for enhancing peri-implant epithelial sealing and preventing peri-implantitis.

The present application relates to a kind of compositions for improving skin color and its application, belong to the field of cosmetics.The present application provides a kind of composition, the composition includes recombinant humanization type IV collagen and lavender exosome, the amino acid sequence of the recombinant humanization type IV collagen is as shown in SEQ ID NO.1, both compound use, can increase the expression of heat shock protein, improve cell viability under heat stress, inhibit the expression of endothelin and the production of glycation product, to improve the effect of skin skin color, and both have synergistic effect.The composition of the present application reduces the amount of recombinant humanization type IV collagen under the premise of achieving similar effects by using lavender exosome, significantly reduces the application cost of recombinant humanization type IV collagen.

The invention belongs to the technical field of oral care, and discloses application of beta-hydroxybutyric acid and an oral care product. The invention provides a novel application of beta-hydroxybutyric acid. The beta-hydroxybutyric acid is found to be capable of effectively promoting the expression of I-type collagen, III-type collagen, IV-type collagen and VII-type collagen; therefore, the oral mucosa healing can be effectively promoted, the oral mucosa barrier is repaired and strengthened, and the oral mucosa repair and gingival repair are promoted; and the oral care product with any one of the effects of promoting oral mucosa healing, repairing and strengthening oral mucosa barrier, promoting oral mucosa repair and repairing gingiva is formed.

The invention belongs to the technical field of medicines, and particularly relates to a mitochondria-rich extracellular vesicle derived from periodontal cells and application of the extracellular vesicle in ischemic brain injury. The extraction method of the extracellular vesicles comprises the following steps: (1) chopping a cerebral cortex part, adding type II collagenase and DNaseI for digestion, and then adding a gradient separation medium for separation to obtain pericytes; (2) spreading the periodontal cells on a culture dish coated with IV-type collagen, and culturing in a periodontal cell culture medium; and (3) when the confluence degree of the cells reaches 80%-90%, replacing the cell culture medium with a fresh culture medium without exosome FBS, reoxygenating the cells for 24 hours after OGD treatment, collecting culture supernatant, and centrifuging the culture supernatant to obtain the extracellular vesicles. It is found for the first time that the PC-EVs obtained after OGD stress can protect the brain from ischemic stroke injury, and the PC-EVs have important reference significance for researching related drugs for treating ischemic brain injury.

The present invention relates to a method for manufacturing a biosynthetic human structural material. The present invention provides a recombinant type IV humanized collagen in which the amino acid sequence contains n (n is an integer of 1 or more) repeating sequences, and the repeating sequence is the sequence of the functional region of human native type IV collagen. The present invention provides the amino acid sequence of a recombinant humanized type IV collagen whose amino acid sequence is derived from native human type IV collagen, does not cause a harmful immune reaction even when administered to the human body, can be accurately expressed in vitro, and has succeeded in biosynthesis in vitro. The recombinant type IV humanized collagen prepared in the present invention has a higher cell adhesion effect than commercially available human-derived collagen. Further, the manufacturing method provided by the present invention is simple, and recombinant type IV humanized collagen can be manufactured at low cost and in high yield.

The application provides a cyclic hexapeptide compound, a preparation method and application thereof. The cyclic hexapeptide compound has the structure of formula (I), has higher stability and bioavailability, can significantly promote the secretion of collagen, especially type I collagen, type III collagen and type IV collagen, has a tightening effect, and can be applied to cosmetics to maintain the structure and elasticity of skin and promote skin health.

Disclosed herein is a split intein system including a first nucleic acid molecule encoding a fusion protein including an N-extein of an extein pair and an N-intein of an intein pair, where the N-extein includes a first signal sequence N-terminal to an N-terminal portion of a type IV collagen alpha chain. Also disclosed is a second nucleic acid molecule encoding a fusion protein including a second signal sequence, a C-intein of the intein pair, and a C-extein of the extein pair, where the C-extein includes a C-terminal portion of the type IV collagen alpha chain. Further disclosed are AAV vectors including the nucleic acid molecules, and methods of using the AAV vectors or nucleic acid molecules to treat a subject with Alport syndrome.

The invention discloses a preparation method and application of recombinant humanized IV-type collagen, particularly discloses an optimized nucleotide sequence for coding M6 protein, and discovers that a carrier containing a plurality of optimized nucleotide sequences and a carrier containing CPB and KEN2 nucleotide sequences are co-transformed into cells through a large amount of screening, so that the recombinant humanized IV-type collagen is obtained. The expression quantity of the M6 protein can be obviously improved, and the obtained M6 has very good cell proliferation and migration activity.

The invention discloses a recombinant IV type collagen capable of being used for adjuvant therapy of xerodermia. The amino acid sequence of the recombinant IV type collagen is as shown in SEQ ID No. 1. The novel recombinant IV type collagen provided by the invention has the advantages that the yield is high; in addition, synthesis of lipid-related enzymes is adjusted, synthesis and secretion of lipid in sebaceous gland cells are adjusted, completeness of skin barriers is maintained, keratinocyte gaps are filled, and hydrophobic barriers are formed; the biological activity or the effect related to moisturizing and skin dryness relieving such as moisture loss prevention is obviously superior to that of commercialized human IV type collagen or other truncated proteins of the human IV type collagen alpha1 chain.

An assay measuring protease mediated degradation of type IV collagen and its biomarker potential for identifying cancer patients with a T-cell permissive tumor microenvironment is described.

Described herein is an assay for measuring protease-mediated degradation of type IV collagen and its biomarker potential for identifying cancer patients with a T-cell permissive tumor microenvironment.