50 results about "Obstructive azoospermia" patented technology

The invention belongs to the fields of gene engineering and reproductive medicine, and discloses a single nucleotide polymorphism (SNP) marker related with clinically cryptogenic non-obstructive azoospermia aided diagnosis and application of the SNP marker. The marker is a combination of rs1014331, rs10765875, rs10889203, rs11066453, rs11167809, rs12097821, rs1990264, rs3000811, rs3130048, rs391129, rs4716515, rs658239, rs6589102, rs7042232, rs7099208, rs7144703, rs7194 and rs985421. The marker can be used for preparing a clinically cryptogenic non-obstructive azoospermia aided diagnosis kit.

The invention which belongs to the medical field of genetic engineering and reproduction discloses a seminal plasma miRNA marker associated with human non-obstructive azoospermia and an application thereof. The maker is selected from several of hsa-miR-141, hsa-miR-193a-5p, hsa-miR-590-5p and hsa-miR-7-1*. The maker which has specificities and sensitivities to the non-obstructive azoospermia can be used for the preparation of a reagent for non-obstructive azoospermia diagnosis or monitoring, so invasive diagnosis is avoided, repeated detection is realized, and the dynamic monitoring of the obstacle degree of sperm generation can be easily achieved.

The invention discloses a separation and culture method and application of human testis mesenchymal stem cells. The separation method comprises the steps that an obstructive azoospermia patient testis specimen which is processed through tissue biopsy separation is digested with type IV collagenase, digested cell suspension is filtered to remove tissue clumps, and human testis cells are obtained; the human testis cells are marked with a CD51 antibody, separation is performed with a flow cytometer, the cells expressing the CD51 are collected, and then the human testis mesenchymal stem cells with the positive CD51 are separated. The invention further provides the human testis mesenchymal stem cells which are obtained through the separation method and have the positive CD51, the culture method of the stem cells and application of the stem cells in preparation of medicine for treating related diseases caused by low testosterone level.

The invention relates to a reagent kit for detecting obstructive and non-obstructive azoospermia based on an Eppin antibody. The reagent kit comprises bovine serum albumin, a phosphate buffer solution, a TBST eluant, a rabbit anti-human Eppin antibody, a goat anti-rabbit IgG antibody, a chromogenic agent, a microfilter and a pyroxylin membrane arranged in the microfilter; in testing, seminal plasma which is to be tested is diluted through the phosphate buffer solution, is adsorbed and fixed on the pyroxylin membrane of the microfilter and is closed through the bovine serum albumin; and the pyroxylin membrane closed by the albumen is subjected to first washing through the TBST eluant, is incubated through the rabbit anti-human Eppin antibody, is subjected to secondary washing through TBST, is incubated through the goat anti-rabbit IgG antibody and is subjected to chromogenic discrimination through the chromogenic agent. The reagent kit has the advantages that the reagent kit has no wound, can alleviate the pain of a patient receiving seminal duct radiography and testicle biopsy, can save mass inspection expense needed by the prior seminal duct radiography and relevant inspection, has high sensitivity, can reach the molecular level, is simple, feasible and rapid and has important application prospect and practical value.

The invention discloses seminal plasma exosome tsRNA markers related to non-obstructive azoospermia diagnosis and application of the seminal plasma exosome tsRNA markers. The markers are tRF-Pro-AGG-003 and tRF-Val-AAC-010, the sequence of tRF-Pro-AGG-003 is SEQ ID NO.1, and the sequence of tRF-Val-AAC-010 is SEQ ID NO.2. The markers have specificity and sensitivity on non-obstructive azoospermiaand can be used for preparing a diagnostic kit for non-obstructive azoospermia. The two seminal plasma exosome markers tRF-Pro-AGG-003 and tRF-Val-AAC-010 are differentially expressed in seminal plasma of patients suffering from non-obstructive azoospermia and obstructive azoospermia, non-obstructive azoospermia can be well diagnosed, an azoospermia sperm taking result can be predicted, and the effect of the markers is superior to the indexes such as the common plasma follicle stimulating hormone level and the testicular volume.

The invention discloses a rapid typing detection kit for ejaculated sperm cells. The kit comprises a PBS solution, a calcium-free HBSS buffer solution, a fixing solution, a staining reagent and a quality control product. The PBS solution and the calcium-free HBSS buffer solution both comprise one or more of bovine serum albumin, fetal bovine serum, glycerol serum, protein and glycerol; the staining reagent comprises a cell permeabilizing agent and a fluorescently labeled antibody. The invention also provides a rapid typing detection method for ejaculated sperm cells by adopting the kit mentioned above. The rapid typing detection kit for ejaculated sperm cells and the rapid typing detection method for ejaculated sperm cells can be used for quickly determining the types and the quantity of spermatogenic cells in the ejaculated sperm cells, further determining whether the ejaculated sperm cells are obstructive or non-obstructive azoospermia, solve the problem that clinical evaluation aiming at the current semen cytology in China lacks a quick and accurate detection reagent in the current clinic, provide guidance for a clinician to select a treatment scheme, and practically solve the problem of a patient.

The invention discloses novel application of 12-HEPE or pharmaceutically acceptable fatty acid thereof, and comprises the application of the 12-HEPE or the pharmaceutically acceptable fatty acid thereof in preparation of a medicine for treating NOA. The 12-HEPE or the pharmaceutically acceptable fatty acid thereof can play a therapeutic role by promoting proliferation and differentiation of the endogenous spermatogonium of a mouse damaged by busulfan, and experiments prove that recovery of the thickness and the integrity of the spermatogenic epithelium of the testis and the recovery of the sperm concentration of the tail of epididymiscan be observed through intragastric administration of the 12-HEPE into the NOA mouse induced by the busulfan, meanwhile, proliferation and differentiation of spermatogonium are promoted, expression of the paracrine factors of cells is supported, so that the 12-HEPE can be used as a new target for alleviating the spermatogenesis dysfunction of the testis of the NOA mouse, and a new method is provided for treating the non-obstructive azoospermia.

The invention belongs to the field of analytical chemistry and clinical medicine, and discloses application of mercury and cadmium mixed exposure detection in auxiliary diagnosis of non-obstructive azoospermia. An exposed biomarker related to the non-obstructive azoospermia is mercury and cadmium mixed exposure. The concentration of mercury and cadmium in whole blood is detected by ICP-MS detection, the application can be used for auxiliary diagnosis of the non-obstructive azoospermia, a few amount of blood is used, and the application is simple, rapid and accurate to operate and has relatively good clinical promotion value.

The invention discloses a non-obstructive azoospermia auxiliary diagnosis gene detection kit, relates to the field of azoospermia diagnosis, and solves the problem of difficult clinical diagnosis of male infertility. The non-obstructive azoospermia auxiliary diagnosis gene detection kit comprises a specific PCR primer, a PCR buffer solution, dNTPs and DNA polymerase, wherein the specific PCR primer is a group of specific primers for detecting the 69826819 mutation site in the non-obstructive azoospermia TEX11 gene. A specific primer SEQ ID NO: 1 and a specific primer SEQ ID NO: 2 are designed,the specific primers have higher specificity, the non-obstructive azoospermia auxiliary diagnosis gene detection kit consisting of the specific primers is adopted to detect the 69826819 mutation sitein the TEX11 gene of a male patient, and the detection rate of the gene mutation is 100%.

The invention discloses an application of a serum miRNA combination as molecular marker for assessing non-obstructive azoospermia. The serum miRNA combination includes hsa-miR-1263, hsa-miR-221-5p, hsa-miR-483-3p, hsa-miR-4275 and hsa-miR-194-3p. Through the miRNA combination, a reagent kit for assessing the non-obstructive azoospermia is prepared. The serum miRNA for assessing the non-obstructiveazoospermia is obtained, further a detection reagent kit containing primers used for quantitative RQ-PCR detection of the serum miRNA combination is obtained, detection is performed through the detection primers and the detection reagent kit, the operation is simple, the accuracy is high, and the serum miRNA combination is worth of extensive popularization.

The invention discloses a preparation method and application of a chemiluminiscence detection kit for quantitatively detecting TEX101 concentration, belongs to the field of in-vitro diagnostic reagents, and provides a TEX101 chemiluminiscence detection kit by researching TEX101 concentration difference results of obstructive azoospermia OA and non-obstructive azoospermia NOA. The kit can rapidly and quantitatively detect the concentration of TEX101 in seminal fluid, the sensitivity of identifying obstructive azoospermia OA and non-obstructive azoospermia NOA is 73%, the specificity is 100%, and the kit has a good prediction value for assisted reproduction results.

The invention discloses a CCL8 protein serving as a biomarker of non-obstructive azoospermia and application of the CCL8 protein. The biomarker comprises a CCL8 protein. The invention finds that the concentration of the CCL8 protein in the seminal fluid of the NOA patient is obviously lower than that of a normal male for the first time, and proposes that the CCL8 protein can be used as a potential early warning molecule for evaluating the spermatogenic dysfunction of the NOA patient, so that expansion and diagnosis of NOA indexes are facilitated, the diagnosis range is expanded, and the CCL8 protein has important significance in the field of NOA diagnosis.

The invention discloses a biomarker related to non-obstructive azoospermia and an application of the biomarker. The biomarker comprises an SHISA5 protein. According to the present invention, the concentration of the Wnt receptor inhibition protein SHISA5 in the NOA patient semen is significantly lower than the concentration of the Wnt receptor inhibition protein SHISA5 in the normal male semen for the first time, and the SHISA5 protein can be adopted as the potential early warning molecule for evaluating the spermatogenic dysfunction of the NOA patient, such that the NOA diagnosis index can be easily expanded so as to expand the diagnosis range, and the important significance is provided for the NOA diagnosis field.

The invention discloses plasma exosome miRNA markers related to diagnosis of primary non-obstructive azoospermia and application thereof. The markers are hsa-miR-513c-5p and hsa-miR-202-5p, the sequence of the hsa-miR-513c-5p is shown as SEQ ID NO.1, and the sequence of the hsa-miR-202-5p is shown as SEQ ID NO.2. The markers have specificity and sensitivity to the non-obstructive azoospermia, andcan be used to prepare a diagnostic kit for the non-obstructive azoospermia. The two plasma exosome markers hsa-miR-513c-5p and hsa-miR-202-5p disclosed by the invention are differentially expressed in the plasma of patients with non-obstructive azoospermia and obstructive azoospermia, so that the non-obstructive azoospermia can be diagnosed better, the sperm taking result of azoospermia can be predicted, and the effect is superior to common indexes such as plasma follicle-stimulating hormone level and testis volume.

The invention belongs to the fields of gene engineering and reproductive medicine, and discloses a low-frequency SNV (single nucleotide variant) marker related to assisted diagnosis of NOA (non-obstructive azoospermia) with unknown clinical reasons and application thereof. The marker is a combination of 32 low-frequency SNVs, including rs2294228, rs2180233, rs41360247, rs895497, rs6806528, rs3130785, rs117524265, rs9369640, rs34902660, rs2298090, rs13194053, rs11965377, rs3893259, rs9261588, rs1264429, rs2523480, rs2524276, rs2255221 and the like. The marker can be used for preparing an assisted diagnosis kit for non-obstructive azoospermia with unknown clinical reasons.

The invention provides application of an RBBP7 gene/protein as a drug target in preparation of a product for diagnosing and treating male infertility diseases. Experiments show that the RBBP7 mutant gene/protein causes male infertility, spermatogenesis disorder, spermatogenesis arrest and no sperm. The RBBP7 mutant gene/protein and one pathogenic mutation site of the RBBP7 mutant gene/protein can be used as a target gene for diagnosing male infertility. Meanwhile, the expression level of the RBBP7 of the RBBP7 gene/protein is remarkably reduced in non-obstructive azoospermia patients, and male infertility can be prevented and/or treated by improving the activity and/or expression quantity of the RBBP7 protein.

Application of seminal plasma extracellular vesicle SLC5A12 protein belongs to the field of protein detection. In the application, protein carried by sEV of NS, NOA and OA groups is quantitatively analyzed through adopting a TMT labeling technology, inter-group differential protein is identified, the biomarker SLC5A12 is successfully verified through a PRM technology, and the protein can be used for diagnosing and distinguishing non-obstructive azoospermia and obstructive azoospermia.

The invention discloses pathogenic mutation of genetic gametogenesis disorder and a detection reagent thereof. The invention relates to a mutant Pof1b gene, the mutant Pof1b gene is located in a chromosome X and is deletion mutation c.312_316delp.S104fs, the gene number of the wild type Pof1b gene in an Ensemble database is ENSG00000124429, the physical position of the deletion mutation Pof1b geneis a third exon, five bases TACAT are deleted at chrX-84622737-84622742, and following sequences cause frameshift mutation. The mutant Pof1b gene or the mutant POF1B protein is used as a detection target to be applied to preparation of an auxiliary diagnosis reagent or detection equipment for male non-obstructive azoospermia or female hereditary primary ovarian insufficiency.