343 results about "Male infertility" patented technology

The present invention discloses methods for isolating, proliferating and differentiating germ-line stem cell in vitro. More specifically, the present invention discloses a method for the in vitro isolation and proliferation of mammalian germ-line stem cells, characterized in culturing cells isolated from mammalian testis in an embryonic stem cell culture medium, and for the in vitro differentiation, characterized in encapsulating mammalian germ-line stem cells and Sertoli cells with calcium alginate and co-culturing with peritubular cells. Also, the present invention discloses germ-line stem cells obtained by above methods, a composition for the treatment of male infertility, comprising the germ-line stem cells, and a method for the treatment of male infertility by the use of the germ-line stem cells.

The invention relates to a special clinical nutritional formula for male sterility. The special clinical nutritional formula is prepared from 15-40 parts of protein, 2-8 parts of fat, 40-55 parts of carbohydrates, 5.8-8 parts of dietary fibers, 0.6-1.2 parts of major elements, 0.01-0.04 part of minor elements, 0.005-0.02 part of fat-soluble vitamins, 0.008-0.2 part of water-soluble vitamins, 0.05-1 part of dietary essence, 0.1-2 parts of medicine-food homologous ingredient, 0.1-3 parts of natural plant compound and 0.005-5 parts of new resource food, wherein the medicine-food homologous ingredient is at least one selected from poria cocos, red raspberries, lycium barbarum, dioscorea opposita, wild jujube seeds, oysters and radix glycyrrhizae; all the ingredients are prepared into powder in advance, and the fat and the vitamins are prepared into powder by adopting a microcapsule coating technology. Through screening and proportioning the ingredients, the nutritional formula provided by the invention provides a more reasonable and more comprehensive nutritional support for male sterility sufferers so as to improve potential and occurred malnutrition state; meanwhile, kidney tonifying, spleen invigorating and liver soothing ingredients are added, so that the body condition of the male sterility sufferers is improved, the sperm production capability is promoted, and the sperm viability is improved.

The invention discloses a method for diagnosing male infertility, and discloses a biological marker indicating male infertility, a method for indentifying and detecting the biological marker indicating male infertility, and a kit for diagnosing or detecting male infertility. The method combined with seminal fluid routine inspection helps to realize the accurate diagnosis on male infertility.

The present invention relates to a novel method of treating male infertility such as, for example, idiopathic asthenospermia. In one aspect the present invention relates to the treatment or prevention of male infertility through the administration of a composition containing ubiquinone, ubiquinol or both, and optionally, a member chosen from the group consisting of a reducing agent, a solubilizer, an emulsifier, a surfactant, a triglyceride, an oil, a fat, a lipid, a phospholipid, a carbohydrate, an antioxidant, a carnitine, creatine, an herbal extract, a vitamin, a coenzyme, a mineral, an electrolyte, a pharmaceutical, a water, a carrier, an excipient, and any combination thereof.

Cooling apparatus (and method) for reducing the risk of, and / or alleviating, male infertility, particularly in a heated environment, includes: a pouch to enclose the scrotal area of the male user; a thermoelectric cooling device to be worn by the male user at a distance from the pouch; and a fluid pump also to be worn by the male user for pumping a cooling fluid from the cold side of the thermoelectric cooling device to the pouch, and a cooling fluid over the hot side of the thermoelectric cooling device to dissipate the heat generated therein to the atmosphere. The thermoelectric cooling device and pump are incorporated in a waistband to be worn around the waist of the male user.

The invention discloses a coated carrier and the application thereof in the method for detecting maturity of sperms and separating mature sperms noninvasively; the coated carrier is a carrier coated by hyaluronic acid, or hyalurate or hyaluronic acid derivatives; the detecting method is as follows: preparing specimens; loading the specimens to the coated carrier and covering with microscopic glass; reacting for 5-60 minutes at room temperature; observing by a microscope and counting; and calculating the combination ratio of hyaluronic acid of active sperms. The separating method is as follows: preparing specimens; loading the specimens to the coated carrier, reacting for 5-120 minutes at the temperature of 18-37 DEG C; and collecting the combined sperms. The coated carrier has the advantages of precise and reliable results, simple and fast operation, low cost and good safety performance, and can be used for estimating sperms quality, sperms maturity and analysis of the causes of sterility disease of mails, and is applicable to assisting reproduction or reproduction research center to safely collect mature and active sperms, so that the goal of improving embryo quality greatly is achieved.

The invention discloses a medicament for treating male infertility, which is prepared from astragalus root, Siberian solomonseal rhizome, epimeddium, cnidium fruit, plantain seeds, ginseng, raspberry, deer horn gum, asparagus root, poria cocos, leek seeds, broomrape, anemarrhena rhizome and eucommia bark through steps of disintegrating, grilling, extracting concrete, and the medicament can be prepared into powders, granules, tablets or capsules.

The invention relates to a corning pill which comprises plantain seed, fringed pink, polygonum aviculare, soapstone, earthworm, seaweed, laminaria, pangolin, red flower, peach kernel, honeysuckle, cordate houttuynia, gardenia, scutellaria baicalensis, agilawood, fructus amomi, cowherb seed, elecampane, mangnolia officinalis and fructus aurantii. The corning pill has the major functions of treatingmale genitourinary system diseases including male infertility, prostatitis, orchitis, seminal vesiculitis, male and female urethritis, cystitis and the like.

The invention provides a kit for male azoospermia diagnosis and a use method. The kit contains biological marker detection reagents for measuring different expression levels of plasma miR-15b, and azoospermia is prompted by excessive expression of the miR-15b. Tiny RNA (ribonucleic acid) genes miR-15b playing an important role in the sperm generation process are discovered, the miR-15b is differently expressed in male infertility patients, and significant expression change of the miR-15b can be detected in the plasma. Based on the principle of quantitative PCR (polymerase chain reaction), and the kit is fine in result repeatability, objective, accurate and easily popularized in different detection mechanisms, hospital laboratories, male laboratories and the like.

The invention provides a method for detecting neuraminidase influencing sperm functions. The method is a novel laboratory detecting method for assessing quality and functions of a sperm and can provide detection and clinical consultations for male infertility patients without clear clinical reasons for infertility. The method is characterized in that expressions of neuraminidase in the sperm are visually quantized through single sperm fluorescence strength analysis or are visually observed by means of fluorescence imaging under a microscope, and if the neuraminidase is highly expressed in the sperm, capacitation of the sperm is achieved through external capacitation liquid, and neuraminidase activity is detected in a fluorescence method.

The invention relates to a traditional Chinese medicine composition used for treating male sterility, a preparation method and a quality control method thereof. The traditional medicine composition contains crude drugs such as fruits of Chinese wolfberry, dodder (fried), raspberry, fructus schizandrae (steamed), plantago seeds (fried with salt) and the like. The preparation method comprises an ultrafine grinding technology and the like. The quality control method of the medicine composition comprises a thin-layer identification method for fruits of Chinese wolfberry, dodder, raspberry, fructus schizandrae and plantago seeds as well as a method for measuring the content of schizandrin B in the plantago seeds. The medicine composition has obvious therapeutic effect in treating renal deficiency and lumbago, post-micturition dribble, spermatorrhea, premature ejaculation, impotence and sterility.

Cooling apparatus (and method) for reducing the risk of, and / or alleviating, male infertility, particularly in a heated environment, includes: a pouch to enclose the scrotal area of the male user; a thermoelectric cooling device to be worn by the male user at a distance from the pouch; and a fluid pump also to be worn by the male user for pumping a cooling fluid from the cold side of the thermoelectric cooling device to the pouch, and a cooling fluid over the hot side of the thermoelectric cooling device to dissipate the heat generated therein to the atmosphere. The thermoelectric cooling device and pump are incorporated in a waistband to be worn around the waist of the male user.

The invention provides a method for diagnosing male infertility in unknown aetiology and relates to an analytical test of a body fluid sample. In certain aspects, a biomarker for indicating male infertility in unknown aetiology is included; a method for identifying and detecting the biomarker is included; a kit for diagnosing or detecting male infertility in unknown aetiology is also included. According to the diagnosing method, the simple, accurate and non-invasive diagnosis of male infertility in unknown aetiology can be realized.

The present invention provides a group of mouse spermatogenesis genes, which is an assembly of 89 genes in total, wherein the respective genes transcribe mRNAs from which cDNAs having the respective base sequences of SEQ ID NOs:1 to 89 are synthesized; an Scot-t gene mutation and a protamine-2 gene mutation which are human homologues of genes belonging to the group of mouse genes and associated with human male infertility. The invention also provides a group of mouse spermatogenesis genes and various molecular biological materials relating thereto; various molecular biological materials relating to male infertility-associated gene mutations; and various test methods and diagnostic methods using the same.

The present invention relates to a cistanche health-care tea and its processing method. Its raw material composition includes (by wt%) 30-36% of cistanche stem, 8-10% of curculigo root, 10-15% of polygonatum root, 8-10% of epimedium, 8-10% of lyceum berry and 30-36% of tea. Its processing method includes the following steps: selecting raw materials, cleaning, pulverizing, stirring, granulating and drying. It can be extensively used for curing the diseases of impotence due to insufficiency of the kidney-yang, female sterility, intolerance of cold, lumbago, male infertility due to insufficiency of essence and blood and constipation, etc.

The present invention related to a functional briefs for men is characterized in natural separation and storage of penis and scrotum without any measures or restrictions when wearing the functional briefs for men, and no escape of penis and scrotum from the containing pocket without regard to the shrinkage or the expansion of the penis and the scrotum, or exercise or daily activities. The characteristics explained hereinbefore are advantageous in having a fresh sense of touch in the briefs and in preventing the uncomfortable feelings of the scrotum and male infertility due to sweat and increasing temperature.

A method for preparation pharmaceutical compounds in large quantities consists of copper (I) halide, preferably the chloride complex of nicotinic acid characterized by elemental analysis, spectroscopy and crystallographic methods, used for treatment of muscular dystrophy, myopathy, myasthenia gravis, parkinsonism, Chronic Fatigue Syndrome, Male Infertility and post stroke muscle weakness.Chlorobis (nicotinic acid) copper (I) monohydrate complex, its composition [CuCl (nicotinic acid)2]. H2O, has been prepared and its physical and chemical properties as well as its crystal structure have been investigated for the first time.

The invention discloses a receipt of gypenoside lipidosome consisting of sterol. The receipt comprises gypenoside, a phosphatide material and the sterol. The invention further discloses a preparation method of the gypenoside lipidosome consisting of the sterol. A preparation form of the lipidosome can be a freeze-drying injection, an injection, a transdermal preparation, aerosol, oral liquid, a solution, an eye-drops preparation, a tablet or a capsule. The gypenoside lipidosome has important treatment effects for hyperlipidemia and cardiovascular and cerebrovascular diseases, and furthermore, has positive treatment and prevention effects for tumor, hepatitis, gastritis, gastric and duodenal ulcer, gastroptosis, stomatitis, diabetes mellitus, astriction, haemorrhoids, asthma, cephalagra, acne, stain, gall-stone, numbness of limbs, pachulosis, male infertility, recession of sexual functions, fatness, white hair and baldness. Furthermore, the physical strength of a human body can be enhanced, the immunity of the organism is improved, the state of fatigue can be relieved, the sleep quality can be obviously improved, the appetite can be whetted, and the senescence can be delayed.

The invention discloses a method for breeding Medicago sativa L. male sterile line. The method is characterized by comprising the following steps of: performing interspecific distant hybridization on wild Medicago falcata L. from Xilin Gol grassland of Inner Mongolia which is used as female parents purple alfalfa from Jungar grassland of Inner Mongolia which is used as male parents; obtaining male sterile line Ms-4 from the offsprings; back crossing four male parents with the male sterile line Ms-4; performing cuttage propagation on the male sterile plants in each back cross offspring, and testing the combining ability of the agronomic traits for further selecting two excellent male sterile lines of the Medicago sativa L. with high combining ability and high volume of production from the male sterile plants, wherein the two excellent male sterile lines are named Ms-4-1 and Ms-4-2. The sterile lines obtained by the method have high combining ability, excellent fertility, high male infertility rate up to 100% and stable heredity.

The invention relates to a method for detecting 305 kinds of semen positioning protein of human testicle and epididymis expression related to human procreation. More specifically, the invention provides an important protein group related to sterility caused by human semen maturation and also provides a qualitative and quantitative method for detecting human semen positioning protein on semen as well as in seminal plasma, thereby providing a new way for definitely diagnosing the causes of male sterility.

The invention discloses an epididymal protease inhibitor protein monoclonal antibody and application thereof, which belong to the field of biological engineering. The monoclonal antibody is secreted by hybridoma preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of C200849. The monoclonal antibody can be combined to the epididymal protease inhibitor protein Eppin protein of specific expression in a testis and epididymis tissue by specificity. The monoclonal antibody can be used for preparing male infertility diagnostic reagents, male contraceptives and anti-fertility study biological products.

The invention belongs to the fields of analytical chemistry and clinical medicine, and discloses a urine fatty acid metabolite marker related to idiopathic male infertility, as well as a detection method and application thereof. The marker is urine fatty acid metabolites, namely, tetradecanedioic acid and / or hexadecanedioic acid, and is detected by adopting a UPLC-Q exactive MS method; the marker can be used for auxiliary diagnosis and monitoring of the idiopathic male infertility, has high sensitivity and specificity, and has a clinical popularization value.

The invention belongs to the technical field of vegetable molecular breeding, and particularly relates to an SNP molecular marker related to infertility of capsicum and application thereof. The SNP molecular marker related to the infertility of the capsicum is SNP2, SNP3, SNP4, SNP7 or SNP8, the nucleotide sequence of an SNP site is shown in SEQ ID NO.1-5, and the molecular marker is co-separatedor closely linked to pepper fertility phenotype. The invention also provides a KASP primer for identifying the above SNP molecular marker; the primer can be used for rapidly identifying the pepper fertility phenotype, and can greatly accelerate the transfer process of a novel capsicum male infertility line. The SNP molecular marker related to the infertility of the capsicum developed belongs to acosegregation SNP marker of a nuclear infertility gene, and the plant fertility can be determined in the seedling stage of a pepper, thereby reducing the seed production cost. The marker can also accelerate the transfer process of the novel capsicum male infertility line.

The invention discloses an application of a traditional Chinese medicine composition in preparing a medicament for treating male infertility. The traditional Chinese medicine composition improves a microenvironment for generating sperms by promoting the blood circulation and removing meridian obstructions so as to achieve the purpose of effectively treating the male infertility.

The invention discloses a method for efficiently separating mouse spermatogonial stem cells. The method includes the following steps that firstly, mouse testis are collected, and a testicular cell suspension is prepared through a two-step enzyme digestion method; secondly, the testicular cell suspension is inoculated in a porous plate according to a certain density, and a spermatogonial stem cell culture solution is added for in-vitro culture; thirdly, the culture solution is removed, testis cells of different times are cultured through tryptic digestion, and spermatogonial stem cells are sorted from the testis cells through the magnetic activated cell sorting; fourthly, the sorted spermatogonial stem cells are inoculated to an STO trophoblast, and a spermatogonial stem cell culture solution is added for primary culture and subculture of the spermatogonial stem cells. A mouse spermatogonial stem cell system is built through the method including the steps of separation, culture, re-separation and re-culture. The method can achieve the aim of efficiently enriching the spermatogonial stem cells in vitro, and is economical and easy to implement, so that a direction is provided for protection of rare animal varieties and treatment of male infertility.

The invention provides a kit for evaluating semen quality and a use method thereof. The kit comprises a cell lysis buffer containing no enzyme activity inhibitor, a detecting buffer solution 1, a protease inhibitor cocktail, a reagent A solution, a reagent B solution, bovine serum albumin, a fluorescent reagent, a sialidase standard stock solution and a detecting buffer solution 2. The defecting buffer solution 1 is a phosphate buffer solution or BWW culture solution. The reagent A solution is prepared from 1% of BCA disodium salt, 2% of anhydrous sodium carbonate, 0.16% of sodium tartrate, 0.4% of sodium hydroxide and 0.95% of sodium bicarbonate, the raw materials are mixed, and the pH value is adjusted to be 11.2 to 11.3. The reagent B solution is prepared from 0.4% of copper sulfate. The detecting buffering solution 2 is a phosphate buffer solution containing 0.05 mol / l sodium acetate and 0.25 microgram / microlitre bovine serum albumin. The activity of sialidase in semen is detected, and diagnostic bases and clinical consulting are provided for male infertility patients with unclear clinical reasons.

The invention provides a compound for use in treating or preventing a disease or condition selected from cystic fibrosis, chronic obstructive pulmonary disease (COPD), asthma, mild pulmonary disease, bronchitis, bronchiectasis, idiopathic bronchiectasis, allergic bronchopulmonary aspergillosis, sinusitis, rhinosinusitis, CFTR-related metabolic syndrome (CRMS), pancreatitis, idiopathic chronic pancreatitis and Sjörgren's syndrome, or for use in preventing male infertility caused by congenital absence of the vas deferens, in a patient by modulating CFTR activity, which compound is 9,10-dimethoxy-2-(2,4,6-trimethylphenylimino)-3-(N-carbamoyl-2-aminoethyl)-3,4,6,7-tetrahydro-2H-pyrimido[6,1-a]isoquinolin-4-one or a pharmaceutically acceptable acid addition salt thereof. The invention also provides a composition comprising (i) 9,10-dimethoxy-2-(2,4,6-trimethylphenylimino)-3-(N-carbamoyl-2-aminoethyl)-3,4,6,7-tetrahydro-2H-pyrimido[6,1-a]isoquinolin-4-one or a pharmaceutically acceptable acid addition salt thereof and (ii) a leukotriene receptor antagonist. The invention also provides a composition comprising (i) 9,10-dimethoxy-2-(2,4,6-trimethylphenylimino)-3-(N-carbamoyl-2-aminoethyl)-3,4,6,7-tetrahydro-2H-pyrimido[6,1-a]isoquinolin-4-one or a pharmaceutically acceptable acid addition salt thereof and (ii) a CFTR potentiator or a CFTR corrector.

The invention relates to a new method for analysis of a male sperm survival rate. A semen sample is stained by a gentian violet solution at a certain concentration, a staining result is observed under a microscope, survival sperms and dead sperms are determined through colored difference, and the sperm survival rate is calculated; after a same sample is stained by an eosin Y standard staining method recommended by the WHO, the sperm survival rate is calculated; a statistical difference between the two sperm survival rates obtained by the two methods is tested by statistics, a result shows that the gentian violet normal saline solution staining method can be used for distinguishing the dead sperms and the survival sperms, almost all of the sperms are colored after being stained by the gentian violet normal saline, sperm motility is decreased, so as to help to count and carry out subsequent analysis under the microscope. The result proves that the method by using the gentian violet solution to stain the semen sample can easily distinguish the survival sperms and the dead sperms, can further analyze the sperm survival rate, can also assist in the analysis of the sperm motility, and contributes to identification and analysis of male sterility.

The invention discloses a kit and a PCR amplification method for detection of one pathogenic mutation site of a male infertility Dmc1 gene. The method is characterized in that a modified mutation-detection primer resistant to 3'-5' exonuclease enzyme digestion is used in a PCR reaction in which a high-fidelity DNA polymerase participates, so that the specificity of PCR is improved; and the existing condition of a gel electrophoresis product band can be directly observed to judge the genotype of the Dmc1 gene corresponding mutation site. The detection kit has low requirements on instruments and equipment, is simple in operation, is economic, has no need of sequencing, and can be operated in common molecular biology laboratories.

The invention belongs to the field of analytical chemistry and clinical medicine and discloses an idiopathic male infertility-related medium-chain fatty acid marker in serum and a detection method and application thereof. The marker is capric acid and / or lauric acid, and an UPLC-Q exactive MS method is adopted for detection. The marker can be used for auxiliary diagnosis and monitoring of idiopathic male infertility, has high sensitivity and specificity, and has clinic promotion value.