The invention discloses an adult
sertoli cell-
pancreas islet compound and a preparation method for the same, which solve the problem of influence on
pancreas islet transplantation effect due to the transplant dysfunction because the
pancreas islet separated and purified presently generates hypoxic-ischemic and immunological rejection reactions after being transplanted and then loses the function. The preparation method comprises the following steps of: 1, separating and purifying adult pancreas islet by adopting a semi-automatic filling and continuous
density gradient methods; 2, separating and purifying adult
sertoli cells by adopting a two-step
digestion method; and 3, constructing the pancreas islet-
sertoli cell compound by adopting a specific co-culture method. The adult
sertoli cell-pancreas islet compound and the preparation method for the same disclosed by the invention have the
advantage that pancreas
islet cells and
sertoli cells are coupled into a compound, the compound is used as a transplant, and the
sertoli cells are attached to the surface of the pancreas islet, so as to improve the
nutritional status of the pancreas islet, inhibit rejection reaction, and increase the
survival rate of the pancreas islet; the operation is simple and convenient; and the success rate is high.