7 results about "Leydig cell" patented technology

The application belongs to the field of regenerative medicine and nano drug delivery system, and particularly relates to a drug-loaded exosome for improving the testosterone secretion capacity of testicular interstitial cells and a preparation method and application thereof. The application links a targeting FATE1 polypeptide to the surface of MSC-derived exosomes by a chemical coupling method, and loads a small molecule compound in the exosomes by electroporation, so as to obtain modified MSC-derived exosomes. The obtained exosomes can obtain the targeting capacity in testicular interstitial cells, and the natural repair capacity of MSC-derived exosomes and the release of small molecule drugs promote the repair of damaged cells, improve the testosterone secretion capacity of testicular interstitial cells, and relieve male erectile dysfunction caused by diseases or aging.

The present application relates to the technical field of genetic engineering, and particularly relates to application of miR-27b-3p in preparation of products for regulating cell proliferation and testosterone synthesis. The present application constructs Inhibitor and Mimics sequences with miR-27b-3p gene as a target, and for the first time proposes that overexpression of miR-27b-3p gene can significantly inhibit proliferation and testosterone synthesis of goat testicular interstitial cells, and inhibition of miR-27b-3p gene can significantly promote proliferation and testosterone synthesis of goat testicular interstitial cells. The present application has important significance for improving development of goat testis, and provides technical support for research and development of products for regulating proliferation and testosterone synthesis of goat testicular interstitial cells with miR-27b-3p gene as a target.

The invention relates to application of ginsenoside Rg4 as a unique active ingredient in preparation of a medicine for improving male delayed gonad hypofunction, belonging to the field of biological medicine. According to the invention, the action mechanism of the Rg4 for improving LOH is defined for the first time: the Rg4 can specifically up-regulate the mRNA expression quantity of StAR and StARD7 in a testis tissue, and promote the transport of cholesterol to the inner mitochondrial membrane of a testis Leydig cell from the transcriptional level; the Rg4 improves the mRNA expression quantity of P450scc, 3 beta-HSD in testicular tissues by promoting the efficient transport of cholesterol, enhances the conversion of cholesterol to pregnenolone and the catalytic synthesis of subsequent steroids, and improves the endogenous testosterone synthesis capability; the Rg4 can recover the negative feedback regulation function of an HPG axis, regulate secretion of pituitary LH and FSH to physiological steady state, and avoid abnormal fluctuation of hormone level. The invention fills the technical blank of Rg4 in the field of LOH gene regulation and control.

The present application relates to the technical field of genetic engineering, and particularly relates to application of PPARG in preparation of products for regulating cell proliferation and testosterone synthesis.The present application constructs a super-expression plasmid pcDNA3.1(+)‑PPARG of PPARG gene, simultaneously synthesizes an interference sequence si‑PPARG, and for the first time proposes that overexpression of the PPARG gene can significantly promote proliferation of goat testicular interstitial cells and testosterone synthesis, and inhibition of the PPARG gene can significantly inhibit proliferation of goat testicular interstitial cells and testosterone synthesis.The present application has important significance for improving development of goat testis, and provides technical support for research and development of products for regulating goat testicular interstitial cell proliferation and testosterone synthesis by taking the PPARG gene as a target.

The invention belongs to the technical field of construction of cell models, and particularly relates to a method for constructing a hyperuricemia testicular interstitial cell model. Comprising the following steps: (1) selecting an 8-week-old male C57BL / 6 mouse, dissecting, collecting testis, removing testis albuginea, cutting into fragments, digesting by utilizing collagenase IV, filtering, centrifuging, discarding supernatant, diluting cell suspension, adding luteinizing hormone receptor coupled fluorescein to mark testis interstitial cells, and sorting by utilizing flow cytometry to obtain testis interstitial cell suspension; (2) counting by using a blood cell counting plate, diluting the testicular interstitial cells, inoculating the testicular interstitial cells into a 24-pore plate, and culturing the testicular interstitial cells in a cell culture box; and (3) after 24-48 hours, taking out the 24-well plate, discarding cell supernate, cleaning with PBS, adding a uric acid solution, and continuously culturing to obtain the hyperuricemia testicular interstitial cell model. The obtained interstitial cell model can be used for exploring various function change mechanisms of the testicular interstitial cells in a hyperuricemia environment, and is simple to operate, effective, high in repeatability and short in time consumption.

This invention relates to the fields of environmental toxicology and organoid technology, and particularly to a method for assessing the toxicity of environmental microplastic exposure based on testicular organoids. The method includes: processing waste testicular tissue into a single-cell suspension, the single-cell suspension comprising supporting cells, germ cells, and interstitial cells; seeding the single-cell suspension onto a biomimetic hydrogel scaffold for culture to obtain mature testicular organoids, which are then cultured in a target microplastic exposure solution to establish a microplastic exposure model; determining the raw values ​​of toxicity assessment indicators corresponding to the microplastic exposure model, including: morphological score data, grayscale values ​​of cell marker expression, DNA damage indicators, testosterone secretion levels, and cytotoxicity indicators; calculating a comprehensive toxicity score based on the raw values ​​of each toxicity assessment indicator, and assessing the reproductive toxicity risk level of the target microplastic exposure based on the comprehensive toxicity score. This application can improve the accuracy of assessing the reproductive toxicity risk level of microplastic exposure.

This invention provides the application of lncRNA TCONS_00131158 in regulating testosterone synthesis and assessing reproductive potential in goats, belonging to the field of animal molecular breeding technology. This invention solves the problem of effectively assessing male reproductive potential in goats and the lack of relevant molecular markers. Through histological and transcriptomic analysis of the testes of Qianbei Ma goats at different developmental stages, the long non-coding RNA TCONS_00131158, closely related to the critical window period of testicular development, was identified, and it was confirmed that it regulates testosterone synthesis and interstitial cell function through the miR-30c-3p / SERPINH1 axis. The beneficial effects of this invention are that it provides a molecular marker that can be used for early screening of high-quality breeding rams, and provides a new technical target and intervention strategy for regulating testosterone synthesis and promoting male reproductive function, which has important application prospects in livestock production.