15 results about "Leydig cell" patented technology

The invention belongs to the technical field of biological medicines, and particularly discloses application of roxburgh rose glycoside in preparation of a medicine for improving dyszoospermia. Pharmacodynamic verification is carried out by constructing various animal models with dyszoospermia, and it is found that the roxburgh rose glycoside can significantly improve reproductive function indexes, for example, the roxburgh rose glycoside can significantly increase the density of testicular interstitial cells, the thickness of spermatogenic epithelium and the density of sperms in epididymis lumens. As a main active component of a medicinal and edible plant roxburgh rose, the roxburgh rose glycoside has good biological safety and human body tolerance. The new application of the roxburgh rose glycoside in the field of reproductive medicine is disclosed for the first time, a novel candidate medicine is provided for treating dyszoospermia, and meanwhile, an application system of the roxburgh rose glycoside in functional health care products and healthy food additives is constructed.

The application belongs to the field of regenerative medicine and nano drug delivery system, and particularly relates to a drug-loaded exosome for improving the testosterone secretion capacity of testicular interstitial cells and a preparation method and application thereof. The application links a targeting FATE1 polypeptide to the surface of MSC-derived exosomes by a chemical coupling method, and loads a small molecule compound in the exosomes by electroporation, so as to obtain modified MSC-derived exosomes. The obtained exosomes can obtain the targeting capacity in testicular interstitial cells, and the natural repair capacity of MSC-derived exosomes and the release of small molecule drugs promote the repair of damaged cells, improve the testosterone secretion capacity of testicular interstitial cells, and relieve male erectile dysfunction caused by diseases or aging.

The invention discloses a testis Leydig cell senescence marker and application thereof, the senescence marker is CCN5 protein, the senescence condition of the Leydig cell can be specifically reflected by detecting the CCN5 level of testis tissue, the senescence marker can be used as an index for evaluating Leydig cell senescence and testis senescence, and meanwhile, the senescence marker is an effective supplement for a diagnostic scheme of late onset hypogonadism (LOH). Furthermore, the invention also provides application of a preparation for inhibiting CCN5 protein expression in preparation of a product for improving testis Leydig cell senescence or treating diseases caused by testis Leydig cell senescence, the testis Leydig cell senescence can be improved and the LOH symptom can be treated by reducing CCN5 in a targeted manner, and treatment can be performed aiming at the core phenotypes of'lipid droplet loss' and'fibrosis' of testis Leydig cell senescence. The method is a treatment scheme different from exogenous testosterone supplement, stem cell injection, Senlytics drugs and exosome therapy.

The invention discloses an application of UNC 926 hydrochloride in improving male testosterone. According to the application disclosed by the invention, UNC 926 hydrochloride micromolecules can increase the secretion amount of testosterone by stimulating testicular interstitial cells, so that a brand new thought is provided for clinically improving the development of low-testosterone treatment medicines for males.

The invention belongs to the technical field of biology, and discloses a porcine testicular mesenchymal stem cell immortalized cell line establishment method and application thereof, and the method comprises the following steps: 1) separating porcine testicular mesenchymal stem cells; (2) enriching the swine testis mesenchymal stem cells; and (3) immortalizing the pig testicular mesenchymal stem cells: inoculating the primary pig testicular mesenchymal stem cells, and transferring SV40 large T antigen into the cells by utilizing lentivirus, so that the pig testicular mesenchymal stem cells are immortalized. The pig testicular mesenchymal stem cell immortalized cell line obtained by the invention is uniform in quality, can be stably passaged for more than 60 generations, is cultured for more than 5 months, and can secrete testosterone under the stimulation of LH (luteinizing hormone). Therefore, the immortalized cell line of the porcine testis mesenchymal stem cells is successfully established, abundant cell resources can be provided for future fundamental research and disease treatment, and a foundation is laid for researching the characteristics of the mesenchymal cell lineage.

The invention discloses application of cinnamyl aldehyde, and cinnamyl aldehyde can regulate synthesis and secretion of androgen testosterone and can be applied to related products for regulating synthesis and secretion of androgen testosterone. In addition, tests prove that cinnamyl aldehyde increases synthesis and secretion of testicular interstitial cell androgen by improving activity of a steroid hormone synthesis nuclear receptor promoter and up-regulating expression of steroid hormone synthetase.

The present application relates to the technical field of genetic engineering, and particularly relates to application of miR-27b-3p in preparation of products for regulating cell proliferation and testosterone synthesis. The present application constructs Inhibitor and Mimics sequences with miR-27b-3p gene as a target, and for the first time proposes that overexpression of miR-27b-3p gene can significantly inhibit proliferation and testosterone synthesis of goat testicular interstitial cells, and inhibition of miR-27b-3p gene can significantly promote proliferation and testosterone synthesis of goat testicular interstitial cells. The present application has important significance for improving development of goat testis, and provides technical support for research and development of products for regulating proliferation and testosterone synthesis of goat testicular interstitial cells with miR-27b-3p gene as a target.

The present application provides a complex for protecting a cell and / or tissue graft. The complex comprises a cell mixture and a cell / tissue transplant. The cell mixture is used to protect the cell / tissue graft. The transplantation body is at least one of hepatocytes, islet cells, hematopoietic stem cells, adrenal medullary cells, testicular interstitial cells, follicle cells, gland pituitary cells, thyroid tissue blocks, parathyroid gland tissue blocks and thymus tissue blocks. The cell mixture comprises at least one of mesenchymal stem cells, primary hepatocytes, primary fibroblasts, dendritic cells, primary hepatocytes and fibroblasts. The complex not only can solve the problems of oxygen deficit and lack of nutritional factors in the transplantation process of the transplantation body, but also can reduce the immunological rejection reaction of a receptor to an allosome or xenograft after the transplantation of the transplantation body. The invention further provides a preparation method and application of the complex. By utilizing the product, cells or tissues can be transplanted into the spleen, the abdominal cavity or the position below the greater omentum, so that the application range of the transplant is expanded.

The invention relates to application of ginsenoside Rg4 as a unique active ingredient in preparation of a medicine for improving male delayed gonad hypofunction, belonging to the field of biological medicine. According to the invention, the action mechanism of the Rg4 for improving LOH is defined for the first time: the Rg4 can specifically up-regulate the mRNA expression quantity of StAR and StARD7 in a testis tissue, and promote the transport of cholesterol to the inner mitochondrial membrane of a testis Leydig cell from the transcriptional level; the Rg4 improves the mRNA expression quantity of P450scc, 3 beta-HSD in testicular tissues by promoting the efficient transport of cholesterol, enhances the conversion of cholesterol to pregnenolone and the catalytic synthesis of subsequent steroids, and improves the endogenous testosterone synthesis capability; the Rg4 can recover the negative feedback regulation function of an HPG axis, regulate secretion of pituitary LH and FSH to physiological steady state, and avoid abnormal fluctuation of hormone level. The invention fills the technical blank of Rg4 in the field of LOH gene regulation and control.

The present application relates to the technical field of genetic engineering, and particularly relates to application of PPARG in preparation of products for regulating cell proliferation and testosterone synthesis.The present application constructs a super-expression plasmid pcDNA3.1(+)‑PPARG of PPARG gene, simultaneously synthesizes an interference sequence si‑PPARG, and for the first time proposes that overexpression of the PPARG gene can significantly promote proliferation of goat testicular interstitial cells and testosterone synthesis, and inhibition of the PPARG gene can significantly inhibit proliferation of goat testicular interstitial cells and testosterone synthesis.The present application has important significance for improving development of goat testis, and provides technical support for research and development of products for regulating goat testicular interstitial cell proliferation and testosterone synthesis by taking the PPARG gene as a target.

The invention belongs to the technical field of construction of cell models, and particularly relates to a method for constructing a hyperuricemia testicular interstitial cell model. Comprising the following steps: (1) selecting an 8-week-old male C57BL / 6 mouse, dissecting, collecting testis, removing testis albuginea, cutting into fragments, digesting by utilizing collagenase IV, filtering, centrifuging, discarding supernatant, diluting cell suspension, adding luteinizing hormone receptor coupled fluorescein to mark testis interstitial cells, and sorting by utilizing flow cytometry to obtain testis interstitial cell suspension; (2) counting by using a blood cell counting plate, diluting the testicular interstitial cells, inoculating the testicular interstitial cells into a 24-pore plate, and culturing the testicular interstitial cells in a cell culture box; and (3) after 24-48 hours, taking out the 24-well plate, discarding cell supernate, cleaning with PBS, adding a uric acid solution, and continuously culturing to obtain the hyperuricemia testicular interstitial cell model. The obtained interstitial cell model can be used for exploring various function change mechanisms of the testicular interstitial cells in a hyperuricemia environment, and is simple to operate, effective, high in repeatability and short in time consumption.

The invention discloses a preparation method and application of a testis repair factor preparation, relates to the field of biological preparations, and aims to solve the problem that existing mesenchymal stem cells have a poor treatment effect on testis aging. The method comprises the following steps: 1, taking P1-generation umbilical cord mesenchymal stem cells, and culturing by adopting a special culture medium; 2, culturing the culture solution supernatant obtained after the culture in the step 1 in a Graviite microgravity simulation control system, then transferring the culture solution supernatant into an ultrafiltration tube, and centrifuging for ultrafiltration concentration to obtain a cell culture supernatant concentrated solution; and 3, adding normal saline into the cell culture supernatant concentrate obtained in the step 2 to prepare the testis repair factor preparation. The repair factor preparation prepared by the method has a good protection effect on testis intermediate cells and supporting cells, and can recover testosterone secretion and improve testis hypofunction by improving testis oxidative stress and immunoregulation.

This invention relates to the fields of environmental toxicology and organoid technology, and particularly to a method for assessing the toxicity of environmental microplastic exposure based on testicular organoids. The method includes: processing waste testicular tissue into a single-cell suspension, the single-cell suspension comprising supporting cells, germ cells, and interstitial cells; seeding the single-cell suspension onto a biomimetic hydrogel scaffold for culture to obtain mature testicular organoids, which are then cultured in a target microplastic exposure solution to establish a microplastic exposure model; determining the raw values ​​of toxicity assessment indicators corresponding to the microplastic exposure model, including: morphological score data, grayscale values ​​of cell marker expression, DNA damage indicators, testosterone secretion levels, and cytotoxicity indicators; calculating a comprehensive toxicity score based on the raw values ​​of each toxicity assessment indicator, and assessing the reproductive toxicity risk level of the target microplastic exposure based on the comprehensive toxicity score. This application can improve the accuracy of assessing the reproductive toxicity risk level of microplastic exposure.

This invention provides the application of lncRNA TCONS_00131158 in regulating testosterone synthesis and assessing reproductive potential in goats, belonging to the field of animal molecular breeding technology. This invention solves the problem of effectively assessing male reproductive potential in goats and the lack of relevant molecular markers. Through histological and transcriptomic analysis of the testes of Qianbei Ma goats at different developmental stages, the long non-coding RNA TCONS_00131158, closely related to the critical window period of testicular development, was identified, and it was confirmed that it regulates testosterone synthesis and interstitial cell function through the miR-30c-3p / SERPINH1 axis. The beneficial effects of this invention are that it provides a molecular marker that can be used for early screening of high-quality breeding rams, and provides a new technical target and intervention strategy for regulating testosterone synthesis and promoting male reproductive function, which has important application prospects in livestock production.

The invention relates to the technical field of biological medicine, in particular to application of combination of beta-nicotinamide mononucleotide (NMN) and apigenin (APG) in preparation of a product for delaying senescence of testicular interstitial cells. Compared with single treatment, the combined use of NMN and apigenin can significantly improve the NAD < + > level of aged mice, promote the expression of testosterone synthesis related genes and proteins, promote the synthesis of testosterone in testicular interstitial cells, enhance the cell proliferation activity, and improve the mitochondrial function and antioxidant defense system, thereby delaying the aging of the testicular interstitial cells. The discovery of the invention not only contributes to understanding the regulation mechanism of testosterone synthesis in the aging process, but also provides technical support for developing a new anti-aging strategy.